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Keygen Biotech rnasea
Rnasea, supplied by Keygen Biotech, used in various techniques. Bioz Stars score: 93/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rnasea/product/Keygen Biotech
Average 93 stars, based on 17 article reviews
Price from $9.99 to $1999.99
rnasea - by Bioz Stars, 2020-09
93/100 stars

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Related Articles

Flow Cytometry:

Article Title: Ellagic acid exerts antitumor effects via the PI3K signaling pathway in endometrial cancer
Article Snippet: .. The cells were treated with RNaseA at 37 ℃ for 30 minutes, stained with propidium iodide using a cell cycle detection kit (KeyGen Biotech, Nanjing, China) according to the manufacturer's instructions and then analyzed using a FACSCalibur flow cytometer (BD Biosciences) and BD Cell Quest software. .. For apoptosis analysis, after the cells were seeded, 20 μM EA was added and cultured with the cells for 48 h. Apoptosis was detected with Annex V-APC and propidium iodide (Annexin V-APC Apoptosis Detection Kit, KeyGEN) following the manufacturer's instructions.

Cell Cycle Assay:

Article Title: Oncogenic HSP60 regulates mitochondrial oxidative phosphorylation to support Erk1/2 activation during pancreatic cancer cell growth
Article Snippet: .. Cell cycle analysis Cells (1 × 106 ) were collected, washed twice with PBS, and fixed with 75% cold ethanol at 4 °C for 12 h. After two washes with PBS, cells were co-incubated with 50 μg/mL propidium iodide (Keygen Biotech) and 10 μg/mL RNaseA (Keygen Biotech) at 4 °C for 30 min in the dark. .. Cells were then analyzed for DNA content by FACS using a BD Accuri™ C6 (BD Biosciences) and the results of the cell cycle were analyzed by FlowJo (Tree Star, Ashland, OR, USA).

Cytometry:

Article Title: Ellagic acid exerts antitumor effects via the PI3K signaling pathway in endometrial cancer
Article Snippet: .. The cells were treated with RNaseA at 37 ℃ for 30 minutes, stained with propidium iodide using a cell cycle detection kit (KeyGen Biotech, Nanjing, China) according to the manufacturer's instructions and then analyzed using a FACSCalibur flow cytometer (BD Biosciences) and BD Cell Quest software. .. For apoptosis analysis, after the cells were seeded, 20 μM EA was added and cultured with the cells for 48 h. Apoptosis was detected with Annex V-APC and propidium iodide (Annexin V-APC Apoptosis Detection Kit, KeyGEN) following the manufacturer's instructions.

Incubation:

Article Title: LncRNA HOTAIRM1 Inhibits the Proliferation and Invasion of Lung Adenocarcinoma Cells via the miR-498/WWOX Axis
Article Snippet: .. Subsequently, fixed cells suspended in 50 μg/mL of propidium iodide (KeyGen BioTECH) and 100 μg/mL of RNaseA (KeyGen BioTECH), cells were incubated for 40 min at room temperature and in the absence of light. ..

Article Title: Low BCL9 expression inhibited ovarian epithelial malignant tumor progression by decreasing proliferation, migration, and increasing apoptosis to cancer cells
Article Snippet: .. 100 μl RNaseA (KeyGEN BioTECH, KGA511-KGA512) was added to the cells and incubated at 37 °C for 30 min. Then, propidium iodide (KeyGEN BioTECH, KGA511-KGA512) was added and incubated at 4 °C for 30 min in the dark. .. Using flow cytometry (BD; FACSAria, USA) to analysis the cell samples immediately.

Article Title: Gold-chrysophanol nanoparticles suppress human prostate cancer progression through inactivating AKT expression and inducing apoptosis and ROS generation in vitro and in vivo
Article Snippet: .. Chrysophanol nanoparticle (90 μ M) was applied on LNCap cells for 24 h. Then, all cells were harvested, washed with PBS, fixed and subsequently incubated with RNaseA (KeyGen Biotech). .. Next, the cells were stained with 50 μ g/ml propidium iodide (PI, Sigma-Aldrich) and the readings were obtained in flow cytometer (BD Biosciences, USA).

Article Title: Icaritin induces lytic cytotoxicity in extranodal NK/T-cell lymphoma
Article Snippet: .. Subsequently, cells were incubated with 100 μl RNaseA (KeyGEN, Nanjing, China) at 37°C for 30 min, and then with 400 μl propidium iodide (PI) at 4°C for 30 min in the dark. .. DNA content was analyzed on a FACSCalibur flow cytometer and data were analyzed using the Modfit LT 3.2 software (Verity Software House, ME, USA).

Staining:

Article Title: Ellagic acid exerts antitumor effects via the PI3K signaling pathway in endometrial cancer
Article Snippet: .. The cells were treated with RNaseA at 37 ℃ for 30 minutes, stained with propidium iodide using a cell cycle detection kit (KeyGen Biotech, Nanjing, China) according to the manufacturer's instructions and then analyzed using a FACSCalibur flow cytometer (BD Biosciences) and BD Cell Quest software. .. For apoptosis analysis, after the cells were seeded, 20 μM EA was added and cultured with the cells for 48 h. Apoptosis was detected with Annex V-APC and propidium iodide (Annexin V-APC Apoptosis Detection Kit, KeyGEN) following the manufacturer's instructions.

Article Title: Long non-coding RNA UBE2CP3 enhances HCC cell secretion of VEGFA and promotes angiogenesis by activating ERK1/2/HIF-1α/VEGFA signalling in hepatocellular carcinoma
Article Snippet: .. Subsequently, the cells were stained with propidium iodide supplemented with RNaseA (Keygen Biotech) for 30 min at 37°C. .. The DNA content of the labelled cells was analysed using FACS flow cytometry (BD Biosciences Inc., Franklin Lakes, NJ, USA).

Article Title: HBx-related long non-coding RNA DBH-AS1 promotes cell proliferation and survival by activating MAPK signaling in hepatocellular carcinoma
Article Snippet: .. After fixed in 70% ice-cold ethanol and kept overnight at 4°C, cells were stained with propidium iodide supplemented with RNaseA (Keygen Biotech) for 30 min at 37°C. .. The DNA content of labeled cells was acquired using FACS cytometry (BD Biosciences Inc., Franklin Lakes, NJ, USA).

Software:

Article Title: Ellagic acid exerts antitumor effects via the PI3K signaling pathway in endometrial cancer
Article Snippet: .. The cells were treated with RNaseA at 37 ℃ for 30 minutes, stained with propidium iodide using a cell cycle detection kit (KeyGen Biotech, Nanjing, China) according to the manufacturer's instructions and then analyzed using a FACSCalibur flow cytometer (BD Biosciences) and BD Cell Quest software. .. For apoptosis analysis, after the cells were seeded, 20 μM EA was added and cultured with the cells for 48 h. Apoptosis was detected with Annex V-APC and propidium iodide (Annexin V-APC Apoptosis Detection Kit, KeyGEN) following the manufacturer's instructions.

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    Keygen Biotech rnase a
    ATRi promotes cell cycle arrest. Notes: (A)  shNC- and shATR-transfected cells were exposed to 10 µM CDDP or 25 µM 5-FU for 48 hours, followed by treatment with RNase A+PI and cell cycle evaluation by FCM. ( B)  Statistics assay for cells in S phase.  * P
    Rnase A, supplied by Keygen Biotech, used in various techniques. Bioz Stars score: 93/100, based on 54 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rnase a/product/Keygen Biotech
    Average 93 stars, based on 54 article reviews
    Price from $9.99 to $1999.99
    rnase a - by Bioz Stars, 2020-09
    93/100 stars
      Buy from Supplier

    93
    Keygen Biotech rnase
    ATRi promotes cell cycle arrest. Notes: (A)  shNC- and shATR-transfected cells were exposed to 10 µM CDDP or 25 µM 5-FU for 48 hours, followed by treatment with RNase A+PI and cell cycle evaluation by FCM. ( B)  Statistics assay for cells in S phase.  * P
    Rnase, supplied by Keygen Biotech, used in various techniques. Bioz Stars score: 93/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rnase/product/Keygen Biotech
    Average 93 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    rnase - by Bioz Stars, 2020-09
    93/100 stars
      Buy from Supplier

    Image Search Results


    ATRi promotes cell cycle arrest. Notes: (A)  shNC- and shATR-transfected cells were exposed to 10 µM CDDP or 25 µM 5-FU for 48 hours, followed by treatment with RNase A+PI and cell cycle evaluation by FCM. ( B)  Statistics assay for cells in S phase.  * P

    Journal: Cancer Management and Research

    Article Title: ATR activated by EB virus facilitates chemotherapy resistance to cisplatin or 5-fluorouracil in human nasopharyngeal carcinoma

    doi: 10.2147/CMAR.S187099

    Figure Lengend Snippet: ATRi promotes cell cycle arrest. Notes: (A) shNC- and shATR-transfected cells were exposed to 10 µM CDDP or 25 µM 5-FU for 48 hours, followed by treatment with RNase A+PI and cell cycle evaluation by FCM. ( B) Statistics assay for cells in S phase. * P

    Article Snippet: The cells were then incubated with RNase A at 37°C for 30 minutes and stained with propidium iodide (PI) at 4°C for 30 minutes (cell cycle detection kit; KeyGEN, Jiangsu, China).

    Techniques: Transfection

    ATRi promotes cell cycle arrest. Notes: (A)  shNC- and shATR-transfected cells were exposed to 10 µM CDDP or 25 µM 5-FU for 48 hours, followed by treatment with RNase A+PI and cell cycle evaluation by FCM. ( B)  Statistics assay for cells in S phase.  * P

    Journal: Cancer Management and Research

    Article Title: ATR activated by EB virus facilitates chemotherapy resistance to cisplatin or 5-fluorouracil in human nasopharyngeal carcinoma

    doi: 10.2147/CMAR.S187099

    Figure Lengend Snippet: ATRi promotes cell cycle arrest. Notes: (A) shNC- and shATR-transfected cells were exposed to 10 µM CDDP or 25 µM 5-FU for 48 hours, followed by treatment with RNase A+PI and cell cycle evaluation by FCM. ( B) Statistics assay for cells in S phase. * P

    Article Snippet: The cells were then incubated with RNase A at 37°C for 30 minutes and stained with propidium iodide (PI) at 4°C for 30 minutes (cell cycle detection kit; KeyGEN, Jiangsu, China).

    Techniques: Transfection