rnase inhibitor  (Thermo Fisher)


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    Structured Review

    Thermo Fisher rnase inhibitor
    Rnase Inhibitor, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1533 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rnase inhibitor/product/Thermo Fisher
    Average 99 stars, based on 1533 article reviews
    Price from $9.99 to $1999.99
    rnase inhibitor - by Bioz Stars, 2020-05
    99/100 stars

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    Related Articles

    Luciferase:

    Article Title: Human Cytomegalovirus DNA Polymerase Subunit UL44 Antagonizes Antiviral Immune Responses by Suppressing IRF3- and NF-κB-Mediated Transcription
    Article Snippet: .. Lipofectamine 2000 (Invitrogen), Polybrene (Millipore), puromycin and RNase inhibitor (Thermo), the Dual-Specific Luciferase Assay kit (Promega), SYBR green (Bio-Rad), digitonin (Sigma), and mouse antibodies against Flag and β-actin (Sigma), hemagglutinin (HA) (Covance), phospho-NF-κB p65, phospho-Stat1 (Tyr 701) (9167), and phospho-IRF-3 (4947S) (Cell Signaling Technology), phospho-TBK1 (ab109272) and TBK1 (ab40676) (Abcam), IRF3 (sc-9082), and STAT1 (sc-346) (Santa Cruz Biotechnology) were purchased from their respective manufacturers. .. Antisera against UL44 and UL82 were generated by immunizing rabbits or mice with purified recombinant UL44 or UL82.

    Protease Inhibitor:

    Article Title: RNA Targets Ribogenesis Factor WDR43 to Chromatin for Transcription and Pluripotency Control.
    Article Snippet: .. Transcription regulation underlies stem cell function and development. .. Transcription regulation underlies stem cell function and development.

    Article Title: A long noncoding RNA sensitizes genotoxic treatment by attenuating ATM activation and homologous recombination repair in cancers
    Article Snippet: .. CLIP UV-irradiated cells were collected in lysis buffer (5 mM PIPES [pH 8.0], 85 mM KCl, 0.5% NP40 and 1% SDS, 10 mM EDTA, 50 mM Tris-HCl [pH 8.1]) and supplemented with Protease Inhibitor Cocktail and RNase inhibitor (Thermo Fisher). .. The cell lysates were precleaned with protein G sepharose beads and then incubated with the indicated antibodies or IgG control, rotating at 4°C overnight.

    Purification:

    Article Title: Divergent Effect of Tacalcitol (PRI-2191) on Th17 Cells in 4T1 Tumor Bearing Young and Old Ovariectomized Mice
    Article Snippet: .. Before reverse transcription, RNA from spleen and lymph nodes (from day 14 or 21 as an early phase of tumor progression and 28 as a late phase of tumor progression) was purified by DNAse digestion in the presence of RNase inhibitor (DNase I, RNase-free, Thermo Scientific, Waltham, Massachusetts, USA), using Veriti 9902 thermocycler (Thermo Scientific, Massachusetts, USA). .. In the next step, a reverse transcription reaction was performed using iScript cDNA Synthesis Kit (Bio-Rad Laboratories, Hercules, California, USA) and the following program: 5 min 25ºC, 30 min 42ºC, 5 min 85ºC and 4ºC. cDNA was stored at -20°C for further analysis.

    SYBR Green Assay:

    Article Title: Human Cytomegalovirus DNA Polymerase Subunit UL44 Antagonizes Antiviral Immune Responses by Suppressing IRF3- and NF-κB-Mediated Transcription
    Article Snippet: .. Lipofectamine 2000 (Invitrogen), Polybrene (Millipore), puromycin and RNase inhibitor (Thermo), the Dual-Specific Luciferase Assay kit (Promega), SYBR green (Bio-Rad), digitonin (Sigma), and mouse antibodies against Flag and β-actin (Sigma), hemagglutinin (HA) (Covance), phospho-NF-κB p65, phospho-Stat1 (Tyr 701) (9167), and phospho-IRF-3 (4947S) (Cell Signaling Technology), phospho-TBK1 (ab109272) and TBK1 (ab40676) (Abcam), IRF3 (sc-9082), and STAT1 (sc-346) (Santa Cruz Biotechnology) were purchased from their respective manufacturers. .. Antisera against UL44 and UL82 were generated by immunizing rabbits or mice with purified recombinant UL44 or UL82.

    Concentration Assay:

    Article Title: Carbodiimide reagents for the chemical probing of RNA structure in cells
    Article Snippet: .. A Cy3-labeled 17-mer RNA oligonucleotide (Cy3–5′-GGGGGUUCAAAUCCCUC-3′, including nine total G/Us; Dharmacon) at a final concentration of 100 nM with 1:1000 final volume of SUPERase In RNase inhibitor (Thermo Fisher) was mixed with corresponding concentrations of freshly dissolved CMC or EDC in borate reaction buffer (50 mM Na2 B4 O7 pH 8.0, 100 mM KCl, 5 mM MgCl2 ) to give a final total volume of 20 µL. .. The reaction was carried out in darkness at various time lengths and temperatures, then quenched with sodium acetate (pH 5.2) to a final concentration of 0.3 M. Aliquots of the reaction mix were then run on 20% urea-PAGE at 250 V for 90 min in darkness, and subsequently scanned for the Cy3 fluorophore.

    Lysis:

    Article Title: A long noncoding RNA sensitizes genotoxic treatment by attenuating ATM activation and homologous recombination repair in cancers
    Article Snippet: .. CLIP UV-irradiated cells were collected in lysis buffer (5 mM PIPES [pH 8.0], 85 mM KCl, 0.5% NP40 and 1% SDS, 10 mM EDTA, 50 mM Tris-HCl [pH 8.1]) and supplemented with Protease Inhibitor Cocktail and RNase inhibitor (Thermo Fisher). .. The cell lysates were precleaned with protein G sepharose beads and then incubated with the indicated antibodies or IgG control, rotating at 4°C overnight.

    Cross-linking Immunoprecipitation:

    Article Title: A long noncoding RNA sensitizes genotoxic treatment by attenuating ATM activation and homologous recombination repair in cancers
    Article Snippet: .. CLIP UV-irradiated cells were collected in lysis buffer (5 mM PIPES [pH 8.0], 85 mM KCl, 0.5% NP40 and 1% SDS, 10 mM EDTA, 50 mM Tris-HCl [pH 8.1]) and supplemented with Protease Inhibitor Cocktail and RNase inhibitor (Thermo Fisher). .. The cell lysates were precleaned with protein G sepharose beads and then incubated with the indicated antibodies or IgG control, rotating at 4°C overnight.

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  • 92
    Thermo Fisher gene exp rac1 mm01201653 mh
    QRTPCR-based validation of GM-CSF-induced regulation of the expression of <t>Rac1,</t> MMP9, Calpain2 and TNFα in vitro as well as in vivo . Gene regulation at 24 h following the start of GMCSF exposure in cultured sensory neurons is shown in (A) and in DRGs at different time points following intraplantar GMCSF injection is shown in (B) . * denotes P ≤ 0.05 as compared to vehicle group, One-Way ANOVA followed by Fisher’s LSD Post-hoc analysis, n = 3 mice per group. (C) Schematic representation of the protocol followed to investigate changes in GMCSF-mediated mechanical and thermal hypersensitivity upon inhibition of <t>Rac1</t> or MMP9 or Calpain 2 or TNFα in vivo .
    Gene Exp Rac1 Mm01201653 Mh, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gene exp rac1 mm01201653 mh/product/Thermo Fisher
    Average 92 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    gene exp rac1 mm01201653 mh - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

    94
    Thermo Fisher rac1
    Changes in the invasive phenotype of pancreatic cancer cells following GSK3β inhibition. (A) Phase-contrast microscopic findings (left panels), expression and subcellular localization of F-actin and Rac-1 (middle panels) and their merged images (right panels) in cancer cells along the wound edge (dashed line) were observed in the wound-healing assay in the presence of DMSO or AR-A014418 (AR). Arrows indicate lamellipodia. (B) Changes in the levels of active <t>(Rac1-GTP)</t> and total Rac1 examined by pull-down assay and Western blotting between the cancer cells treated with DMSO (DM) or 10 µM AR-A014418 (AR) for 24 hrs. (C) Changes in the secretion and mRNA expression of MMP-2 examined by gelatin zymography (left panel) and qRT-PCR (right panel) between PANC-1 cells treated with DMSO (DM) or AR-A014418 (AR) for 24 hrs. Values for the relative levels of mRNA expression are shown as means ± SDs of four separate experiments. * p
    Rac1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rac1/product/Thermo Fisher
    Average 94 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    rac1 - by Bioz Stars, 2020-05
    94/100 stars
      Buy from Supplier

    Image Search Results


    QRTPCR-based validation of GM-CSF-induced regulation of the expression of Rac1, MMP9, Calpain2 and TNFα in vitro as well as in vivo . Gene regulation at 24 h following the start of GMCSF exposure in cultured sensory neurons is shown in (A) and in DRGs at different time points following intraplantar GMCSF injection is shown in (B) . * denotes P ≤ 0.05 as compared to vehicle group, One-Way ANOVA followed by Fisher’s LSD Post-hoc analysis, n = 3 mice per group. (C) Schematic representation of the protocol followed to investigate changes in GMCSF-mediated mechanical and thermal hypersensitivity upon inhibition of Rac1 or MMP9 or Calpain 2 or TNFα in vivo .

    Journal: Molecular Pain

    Article Title: Transcriptional mechanisms underlying sensitization of peripheral sensory neurons by Granulocyte-/Granulocyte-macrophage colony stimulating factors

    doi: 10.1186/1744-8069-9-48

    Figure Lengend Snippet: QRTPCR-based validation of GM-CSF-induced regulation of the expression of Rac1, MMP9, Calpain2 and TNFα in vitro as well as in vivo . Gene regulation at 24 h following the start of GMCSF exposure in cultured sensory neurons is shown in (A) and in DRGs at different time points following intraplantar GMCSF injection is shown in (B) . * denotes P ≤ 0.05 as compared to vehicle group, One-Way ANOVA followed by Fisher’s LSD Post-hoc analysis, n = 3 mice per group. (C) Schematic representation of the protocol followed to investigate changes in GMCSF-mediated mechanical and thermal hypersensitivity upon inhibition of Rac1 or MMP9 or Calpain 2 or TNFα in vivo .

    Article Snippet: Taqman assays (Life Technologies, USA) were used for QRTPCR-based quantification of Rac1 (assay ID Mm01201653_mH), Calpain2 (assay ID Mm00486669_m1), MMP9 (assay ID Mm00600164_g1) and TNFα (assay ID Mm00443260_g1).

    Techniques: Expressing, In Vitro, In Vivo, Cell Culture, Injection, Mouse Assay, Inhibition

    In vivo validation of GM-CSF transcriptional targets in DRG neurons in the context of nociceptive sensitization evoked by long-term exposure to GMCSF. Changes in GMCSF-mediated mechanical hypersensitivity upon inhibition of Rac1 (A) , MMP9 (C) , Calpain-2 (E) or TNFα (G) as compared to corresponding vehicle-treated mice are shown. Response frequency to the von Frey filament at 0.16 g force is represented on the Y-axis. Changes in GM-CSF-mediated thermal hypersensitivity upon inhibition Rac1 (B) , MMP9 (D) , Calpain-2 (F) or TNFα (H) as compared to corresponding vehicle-treated mice are shown. Withdrawal latency in seconds to calibrated radiant heat is represented on the Y-axis. * denotes P ≤ 0.05 as compared to basal values, † denotes P ≤ 0.05 relative to corresponding vehicle treated group, One-Way ANOVA with repeated measures followed Fisher’s LSD Post-hoc analysis , n = 6 mice per group.

    Journal: Molecular Pain

    Article Title: Transcriptional mechanisms underlying sensitization of peripheral sensory neurons by Granulocyte-/Granulocyte-macrophage colony stimulating factors

    doi: 10.1186/1744-8069-9-48

    Figure Lengend Snippet: In vivo validation of GM-CSF transcriptional targets in DRG neurons in the context of nociceptive sensitization evoked by long-term exposure to GMCSF. Changes in GMCSF-mediated mechanical hypersensitivity upon inhibition of Rac1 (A) , MMP9 (C) , Calpain-2 (E) or TNFα (G) as compared to corresponding vehicle-treated mice are shown. Response frequency to the von Frey filament at 0.16 g force is represented on the Y-axis. Changes in GM-CSF-mediated thermal hypersensitivity upon inhibition Rac1 (B) , MMP9 (D) , Calpain-2 (F) or TNFα (H) as compared to corresponding vehicle-treated mice are shown. Withdrawal latency in seconds to calibrated radiant heat is represented on the Y-axis. * denotes P ≤ 0.05 as compared to basal values, † denotes P ≤ 0.05 relative to corresponding vehicle treated group, One-Way ANOVA with repeated measures followed Fisher’s LSD Post-hoc analysis , n = 6 mice per group.

    Article Snippet: Taqman assays (Life Technologies, USA) were used for QRTPCR-based quantification of Rac1 (assay ID Mm01201653_mH), Calpain2 (assay ID Mm00486669_m1), MMP9 (assay ID Mm00600164_g1) and TNFα (assay ID Mm00443260_g1).

    Techniques: In Vivo, Inhibition, Mouse Assay

    Changes in the invasive phenotype of pancreatic cancer cells following GSK3β inhibition. (A) Phase-contrast microscopic findings (left panels), expression and subcellular localization of F-actin and Rac-1 (middle panels) and their merged images (right panels) in cancer cells along the wound edge (dashed line) were observed in the wound-healing assay in the presence of DMSO or AR-A014418 (AR). Arrows indicate lamellipodia. (B) Changes in the levels of active (Rac1-GTP) and total Rac1 examined by pull-down assay and Western blotting between the cancer cells treated with DMSO (DM) or 10 µM AR-A014418 (AR) for 24 hrs. (C) Changes in the secretion and mRNA expression of MMP-2 examined by gelatin zymography (left panel) and qRT-PCR (right panel) between PANC-1 cells treated with DMSO (DM) or AR-A014418 (AR) for 24 hrs. Values for the relative levels of mRNA expression are shown as means ± SDs of four separate experiments. * p

    Journal: PLoS ONE

    Article Title: Aberrant Glycogen Synthase Kinase 3? Is Involved in Pancreatic Cancer Cell Invasion and Resistance to Therapy

    doi: 10.1371/journal.pone.0055289

    Figure Lengend Snippet: Changes in the invasive phenotype of pancreatic cancer cells following GSK3β inhibition. (A) Phase-contrast microscopic findings (left panels), expression and subcellular localization of F-actin and Rac-1 (middle panels) and their merged images (right panels) in cancer cells along the wound edge (dashed line) were observed in the wound-healing assay in the presence of DMSO or AR-A014418 (AR). Arrows indicate lamellipodia. (B) Changes in the levels of active (Rac1-GTP) and total Rac1 examined by pull-down assay and Western blotting between the cancer cells treated with DMSO (DM) or 10 µM AR-A014418 (AR) for 24 hrs. (C) Changes in the secretion and mRNA expression of MMP-2 examined by gelatin zymography (left panel) and qRT-PCR (right panel) between PANC-1 cells treated with DMSO (DM) or AR-A014418 (AR) for 24 hrs. Values for the relative levels of mRNA expression are shown as means ± SDs of four separate experiments. * p

    Article Snippet: Treatment with AR-A014418 decreased lamellipodia formation in cancer cells at the wound edge and resulted in diffuse cytoplasmic distribution of Rac1 and F-actin ( ).

    Techniques: Inhibition, Expressing, Wound Healing Assay, Pull Down Assay, Western Blot, Zymography, Quantitative RT-PCR