rnase free proteinase k  (Millipore)


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  • 87

    Structured Review

    Millipore rnase free proteinase k
    Rnase Free Proteinase K, supplied by Millipore, used in various techniques. Bioz Stars score: 87/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rnase free proteinase k/product/Millipore
    Average 87 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rnase free proteinase k - by Bioz Stars, 2020-04
    87/100 stars

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    Related Articles

    Nucleic Acid Electrophoresis:

    Article Title: Stable Extracellular RNA Fragments of Mycobacterium tuberculosis Induce Early Apoptosis in Human Monocytes via a Caspase-8 Dependent Mechanism
    Article Snippet: To prepare highly purified RNA, the material eluted from DEAE-sepharose with 500–1000 mM NaCl was suspended in 0.1 ml of 10 mM ammonium bicarbonate, 1 mM MgCl2 to a final concentration of 0.4 mg/ml and digested overnight at 37°C with RNase-free DNaseI (Sigma-Aldrich), followed by RNase-free Proteinase K (Sigma-Aldrich) for 8 h at 37°C at a final concentrations of 0.04 mg/ml. .. Final purification was performed by preparative gel electrophoresis using a denaturing urea poly-acrylamide gel .

    Flow Cytometry:

    Article Title: Stable Extracellular RNA Fragments of Mycobacterium tuberculosis Induce Early Apoptosis in Human Monocytes via a Caspase-8 Dependent Mechanism
    Article Snippet: The flow-through fraction was collected, concentrated, and washed three times with 20 mM Tris (pH 8.1). .. To prepare highly purified RNA, the material eluted from DEAE-sepharose with 500–1000 mM NaCl was suspended in 0.1 ml of 10 mM ammonium bicarbonate, 1 mM MgCl2 to a final concentration of 0.4 mg/ml and digested overnight at 37°C with RNase-free DNaseI (Sigma-Aldrich), followed by RNase-free Proteinase K (Sigma-Aldrich) for 8 h at 37°C at a final concentrations of 0.04 mg/ml.

    Isolation:

    Article Title: Stable Extracellular RNA Fragments of Mycobacterium tuberculosis Induce Early Apoptosis in Human Monocytes via a Caspase-8 Dependent Mechanism
    Article Snippet: Paragraph title: Isolation of Extracellular RNA ... To prepare highly purified RNA, the material eluted from DEAE-sepharose with 500–1000 mM NaCl was suspended in 0.1 ml of 10 mM ammonium bicarbonate, 1 mM MgCl2 to a final concentration of 0.4 mg/ml and digested overnight at 37°C with RNase-free DNaseI (Sigma-Aldrich), followed by RNase-free Proteinase K (Sigma-Aldrich) for 8 h at 37°C at a final concentrations of 0.04 mg/ml.

    Laser Capture Microdissection:

    Article Title: Stable Extracellular RNA Fragments of Mycobacterium tuberculosis Induce Early Apoptosis in Human Monocytes via a Caspase-8 Dependent Mechanism
    Article Snippet: Lyophilized CF was suspended overnight at 4°C at 1 mg/ml protein concentration in ConcanavalinA (ConA) binding buffer , and passed over a ConA-Sephadex column (Sigma-Aldrich, St. Louis, MO) to remove mannosylated glycoproteins and lipoglycans (LM and LAM). .. To prepare highly purified RNA, the material eluted from DEAE-sepharose with 500–1000 mM NaCl was suspended in 0.1 ml of 10 mM ammonium bicarbonate, 1 mM MgCl2 to a final concentration of 0.4 mg/ml and digested overnight at 37°C with RNase-free DNaseI (Sigma-Aldrich), followed by RNase-free Proteinase K (Sigma-Aldrich) for 8 h at 37°C at a final concentrations of 0.04 mg/ml.

    Centrifugation:

    Article Title: Stable Extracellular RNA Fragments of Mycobacterium tuberculosis Induce Early Apoptosis in Human Monocytes via a Caspase-8 Dependent Mechanism
    Article Snippet: To prepare highly purified RNA, the material eluted from DEAE-sepharose with 500–1000 mM NaCl was suspended in 0.1 ml of 10 mM ammonium bicarbonate, 1 mM MgCl2 to a final concentration of 0.4 mg/ml and digested overnight at 37°C with RNase-free DNaseI (Sigma-Aldrich), followed by RNase-free Proteinase K (Sigma-Aldrich) for 8 h at 37°C at a final concentrations of 0.04 mg/ml. .. RNA was precipitated from the final aqueous phase with 0.1 vol of 3 M sodium acetate (pH 5.3) and 2.5 vol of ethanol, and collected by centrifugation (13,000× g, 30 min).

    Purification:

    Article Title: Stable Extracellular RNA Fragments of Mycobacterium tuberculosis Induce Early Apoptosis in Human Monocytes via a Caspase-8 Dependent Mechanism
    Article Snippet: .. To prepare highly purified RNA, the material eluted from DEAE-sepharose with 500–1000 mM NaCl was suspended in 0.1 ml of 10 mM ammonium bicarbonate, 1 mM MgCl2 to a final concentration of 0.4 mg/ml and digested overnight at 37°C with RNase-free DNaseI (Sigma-Aldrich), followed by RNase-free Proteinase K (Sigma-Aldrich) for 8 h at 37°C at a final concentrations of 0.04 mg/ml. ..

    Concentration Assay:

    Article Title: Stable Extracellular RNA Fragments of Mycobacterium tuberculosis Induce Early Apoptosis in Human Monocytes via a Caspase-8 Dependent Mechanism
    Article Snippet: .. To prepare highly purified RNA, the material eluted from DEAE-sepharose with 500–1000 mM NaCl was suspended in 0.1 ml of 10 mM ammonium bicarbonate, 1 mM MgCl2 to a final concentration of 0.4 mg/ml and digested overnight at 37°C with RNase-free DNaseI (Sigma-Aldrich), followed by RNase-free Proteinase K (Sigma-Aldrich) for 8 h at 37°C at a final concentrations of 0.04 mg/ml. ..

    Protein Concentration:

    Article Title: Stable Extracellular RNA Fragments of Mycobacterium tuberculosis Induce Early Apoptosis in Human Monocytes via a Caspase-8 Dependent Mechanism
    Article Snippet: Lyophilized CF was suspended overnight at 4°C at 1 mg/ml protein concentration in ConcanavalinA (ConA) binding buffer , and passed over a ConA-Sephadex column (Sigma-Aldrich, St. Louis, MO) to remove mannosylated glycoproteins and lipoglycans (LM and LAM). .. To prepare highly purified RNA, the material eluted from DEAE-sepharose with 500–1000 mM NaCl was suspended in 0.1 ml of 10 mM ammonium bicarbonate, 1 mM MgCl2 to a final concentration of 0.4 mg/ml and digested overnight at 37°C with RNase-free DNaseI (Sigma-Aldrich), followed by RNase-free Proteinase K (Sigma-Aldrich) for 8 h at 37°C at a final concentrations of 0.04 mg/ml.

    Binding Assay:

    Article Title: Stable Extracellular RNA Fragments of Mycobacterium tuberculosis Induce Early Apoptosis in Human Monocytes via a Caspase-8 Dependent Mechanism
    Article Snippet: Lyophilized CF was suspended overnight at 4°C at 1 mg/ml protein concentration in ConcanavalinA (ConA) binding buffer , and passed over a ConA-Sephadex column (Sigma-Aldrich, St. Louis, MO) to remove mannosylated glycoproteins and lipoglycans (LM and LAM). .. To prepare highly purified RNA, the material eluted from DEAE-sepharose with 500–1000 mM NaCl was suspended in 0.1 ml of 10 mM ammonium bicarbonate, 1 mM MgCl2 to a final concentration of 0.4 mg/ml and digested overnight at 37°C with RNase-free DNaseI (Sigma-Aldrich), followed by RNase-free Proteinase K (Sigma-Aldrich) for 8 h at 37°C at a final concentrations of 0.04 mg/ml.

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    Millipore water dnase rnase free
    mTOR siRNA infusion impaired rotarod learning. (A) Time spent by vehicle (distillated/deionized water <t>DNase/RNase</t> free) and siRNA-treated mice on the accelerating rotarod for each trial at days 1, 2, and 3. Rotarod learning was analyzed by pooling together the two first or the two last trials/training day of both groups. Data represent the average mean latency to fall expressed in seconds ± S.E.M, n = 3–4 vehicle-treated and siRNA-treated mice. ** P
    Water Dnase Rnase Free, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/water dnase rnase free/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    water dnase rnase free - by Bioz Stars, 2020-04
    99/100 stars
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    mTOR siRNA infusion impaired rotarod learning. (A) Time spent by vehicle (distillated/deionized water DNase/RNase free) and siRNA-treated mice on the accelerating rotarod for each trial at days 1, 2, and 3. Rotarod learning was analyzed by pooling together the two first or the two last trials/training day of both groups. Data represent the average mean latency to fall expressed in seconds ± S.E.M, n = 3–4 vehicle-treated and siRNA-treated mice. ** P

    Journal: Frontiers in Molecular Neuroscience

    Article Title: mTOR signaling contributes to motor skill learning in mice

    doi: 10.3389/fnmol.2014.00026

    Figure Lengend Snippet: mTOR siRNA infusion impaired rotarod learning. (A) Time spent by vehicle (distillated/deionized water DNase/RNase free) and siRNA-treated mice on the accelerating rotarod for each trial at days 1, 2, and 3. Rotarod learning was analyzed by pooling together the two first or the two last trials/training day of both groups. Data represent the average mean latency to fall expressed in seconds ± S.E.M, n = 3–4 vehicle-treated and siRNA-treated mice. ** P

    Article Snippet: Osmotic pumps were prefilled with either distillated/deionized water DNase/RNase free (EMD Millipore, 6 μl/day) or mTOR siRNA (1.44 nmol/day, diluted in 100% ddH2 O DNase/RNase free, Sigma-Aldrich, Oakville, ON, Canada) and mice were receiving a continuous intrastriatal infusion for 14 consecutive days.

    Techniques: Mouse Assay

    Illustration depicting the experimental design. All animals were subjected to the same handling treatment before the experiments. (A) For systemic treatment, mice were injected systemically with vehicle (DMSO) or rapamycin 15 min prior each rotarod training session. (B) For intrastriatal treatment, a bilateral guide cannula was implanted in the dorsal striatum of mice. After the surgery, seven days of recovery were given to the mice before starting the experiments. 15 min prior the first trial of each rotarod training days, injection of vehicle (DMSO) or rapamycin was delivered into the dorsal striatum of both hemispheres. (C) For intrastriatal siRNA infusion, a bilateral connector cannula was installed into the dorsal striatum. Two osmotic mini-pumps were surgically placed subcutaneously on each side of the scapulae and connected to the cannula via a plastic connector. Mice were receiving for 14 consecutive days a continuous intrastriatal infusion with either distillated/deionized water DNase/RNase free or mTOR siRNA. Mice were trained on the rotarod on the 12th, 13th, and 14th days of treatment.

    Journal: Frontiers in Molecular Neuroscience

    Article Title: mTOR signaling contributes to motor skill learning in mice

    doi: 10.3389/fnmol.2014.00026

    Figure Lengend Snippet: Illustration depicting the experimental design. All animals were subjected to the same handling treatment before the experiments. (A) For systemic treatment, mice were injected systemically with vehicle (DMSO) or rapamycin 15 min prior each rotarod training session. (B) For intrastriatal treatment, a bilateral guide cannula was implanted in the dorsal striatum of mice. After the surgery, seven days of recovery were given to the mice before starting the experiments. 15 min prior the first trial of each rotarod training days, injection of vehicle (DMSO) or rapamycin was delivered into the dorsal striatum of both hemispheres. (C) For intrastriatal siRNA infusion, a bilateral connector cannula was installed into the dorsal striatum. Two osmotic mini-pumps were surgically placed subcutaneously on each side of the scapulae and connected to the cannula via a plastic connector. Mice were receiving for 14 consecutive days a continuous intrastriatal infusion with either distillated/deionized water DNase/RNase free or mTOR siRNA. Mice were trained on the rotarod on the 12th, 13th, and 14th days of treatment.

    Article Snippet: Osmotic pumps were prefilled with either distillated/deionized water DNase/RNase free (EMD Millipore, 6 μl/day) or mTOR siRNA (1.44 nmol/day, diluted in 100% ddH2 O DNase/RNase free, Sigma-Aldrich, Oakville, ON, Canada) and mice were receiving a continuous intrastriatal infusion for 14 consecutive days.

    Techniques: Mouse Assay, Injection

    mTOR siRNA infusion inhibited striatal mTOR expression and decreased the levels of phosphorylated P70S6K and 4EBP1. (A) Levels of total striatal mTOR were evaluated by western blot after the infusion of vehicle (distillated/deionized water DNase/RNase free) or mTOR siRNA. The data, expressed relative to GAPDH, represent the mean of relative optical density of total mTOR (expressed as a percentage of control values) ± S.E.M, n = 3–4; triplicate experiments for each mouse/group. ** P

    Journal: Frontiers in Molecular Neuroscience

    Article Title: mTOR signaling contributes to motor skill learning in mice

    doi: 10.3389/fnmol.2014.00026

    Figure Lengend Snippet: mTOR siRNA infusion inhibited striatal mTOR expression and decreased the levels of phosphorylated P70S6K and 4EBP1. (A) Levels of total striatal mTOR were evaluated by western blot after the infusion of vehicle (distillated/deionized water DNase/RNase free) or mTOR siRNA. The data, expressed relative to GAPDH, represent the mean of relative optical density of total mTOR (expressed as a percentage of control values) ± S.E.M, n = 3–4; triplicate experiments for each mouse/group. ** P

    Article Snippet: Osmotic pumps were prefilled with either distillated/deionized water DNase/RNase free (EMD Millipore, 6 μl/day) or mTOR siRNA (1.44 nmol/day, diluted in 100% ddH2 O DNase/RNase free, Sigma-Aldrich, Oakville, ON, Canada) and mice were receiving a continuous intrastriatal infusion for 14 consecutive days.

    Techniques: Expressing, Western Blot