Structured Review

Promega rnase free dnasei
Rnase Free Dnasei, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rnase free dnasei/product/Promega
Average 99 stars, based on 1 article reviews
Price from $9.99 to $1999.99
rnase free dnasei - by Bioz Stars, 2021-04
99/100 stars

Images

Related Articles

Recombinant:

Article Title: Characterization of the Ebola virus nucleoprotein-RNA complex
Article Snippet: The nucleic acid with mock treatment ran as a smear with bands around 500–1000 nt (Fig. ) and was unaffected by DNase I treatment (Fig. ), but was digested by RNase A treatment (Fig. ), demonstrating that the recombinant NP helix binds to non-viral RNAs, similar to recombinant paramyxo- and rhabdovirus NPs. .. To examine whether the NP-associated RNA in the NP helix is protected from RNase digestion, as occurs with paramyxoviruses , the recombinant NP–RNA complex was treated with a high concentration of RNase A (10 ng μl−1 ) in PBS at 37 °C for 30 min. After excess RNase inhibitor (160 U; Promega) was added, the RNA was phenol/chloroform extracted from the complex as described above. .. Unlike the recombinant NP–RNA complexes of paramyxoviruses , but as has been reported for some rhabdoviruses ( ; ), the NP-associated RNA was sensitive to RNase A and was digested (Fig. ).

Concentration Assay:

Article Title: Characterization of the Ebola virus nucleoprotein-RNA complex
Article Snippet: The nucleic acid with mock treatment ran as a smear with bands around 500–1000 nt (Fig. ) and was unaffected by DNase I treatment (Fig. ), but was digested by RNase A treatment (Fig. ), demonstrating that the recombinant NP helix binds to non-viral RNAs, similar to recombinant paramyxo- and rhabdovirus NPs. .. To examine whether the NP-associated RNA in the NP helix is protected from RNase digestion, as occurs with paramyxoviruses , the recombinant NP–RNA complex was treated with a high concentration of RNase A (10 ng μl−1 ) in PBS at 37 °C for 30 min. After excess RNase inhibitor (160 U; Promega) was added, the RNA was phenol/chloroform extracted from the complex as described above. .. Unlike the recombinant NP–RNA complexes of paramyxoviruses , but as has been reported for some rhabdoviruses ( ; ), the NP-associated RNA was sensitive to RNase A and was digested (Fig. ).

other:

Article Title: Construction of Corynebacterium glutamicum cells as containers encapsulating dsRNA overexpressed for agricultural pest control
Article Snippet: Total RNA (1.6 μg) extracted from dsRNA-producing cells was digested at 25 °C for 30 min in 15 μL reaction solution with 1000 U of RNase A.

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Promega dna dnasei
    Chromatin markers in the casein gene cluster region in different tissues and developmental stages. This illustration is a close-up of the CSN region from Figure 1 . ( A ) ChIP-seq reads for H3K4me2 in MEC isolated from virgin mammary glands at 12 weeks of age, staged at diestrus ( Virgin MG , black), in lactating mammary glands ( Lactating MG , blue) and in liver tissue ( Liver , red). A summary of peaks identified by MAC and SICER are at the top of each of the Virgin MG, Lactating MG, and Liver panels. In the evolutionary conserved region ( ECR ) panel, ECRs are shown in green; ECR1, 6 16 and 21 are indicated in pink. In the bottom panel, the locations of the casein genes (black) and non-casein genes (gray) are indicated by solid arrows denoting the direction of transcription. ( B ) Summary of markers of epigenetic regulation in the CSN region. ( H3Ac ) Summary of results of ChIP for H3-acetylation on lactating mammary gland ( Lact , blue) and liver tissue ( Lvr , red). Sites investigated are indicated by rectangles: filled rectangles, H3AC enriched; open rectangle, non-enriched (See figure S3 ). ( DHS ) Summary of <t>DNaseI</t> Hypersensitivity analysis in MEC isolated from from virgin mammary glands at 12 weeks of age, staged at diestrus ( Virg , black), in lactating mammary gland ( Lact , blue) and liver tissue ( Lvr , red). Sites investigated are indicated by rectangles: filled rectangle, DHS; open rectangle, non-DHS (see Fig. S4 ). ( DNAme ) Summary of results of <t>DNA</t> methylation analysis on lactating mammary gland ( Lact ) and liver tissue ( Lvr ) (see also Fig. 5). Levels of DNA methylation are based on Fig. 5A and Table S2 and are color coded as follows: 0–20% lightest blue, 21–40% light blue, 41–60% blue, 61–80% dark blue and 80–100% black; open rectangles: not analyzed. ( K9me2 ) Summary of ChIP-chip for H3K9me2, a histone modification associated with closed chromatin, on liver tissue from [62] ; red filled rectangle indicates enrichment of H3K9me2 in liver tissue.
    Dna Dnasei, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna dnasei/product/Promega
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dna dnasei - by Bioz Stars, 2021-04
    99/100 stars
      Buy from Supplier

    97
    Promega dnasei
    Chromatin markers in the casein gene cluster region in different tissues and developmental stages. This illustration is a close-up of the CSN region from Figure 1 . ( A ) ChIP-seq reads for H3K4me2 in MEC isolated from virgin mammary glands at 12 weeks of age, staged at diestrus ( Virgin MG , black), in lactating mammary glands ( Lactating MG , blue) and in liver tissue ( Liver , red). A summary of peaks identified by MAC and SICER are at the top of each of the Virgin MG, Lactating MG, and Liver panels. In the evolutionary conserved region ( ECR ) panel, ECRs are shown in green; ECR1, 6 16 and 21 are indicated in pink. In the bottom panel, the locations of the casein genes (black) and non-casein genes (gray) are indicated by solid arrows denoting the direction of transcription. ( B ) Summary of markers of epigenetic regulation in the CSN region. ( H3Ac ) Summary of results of ChIP for H3-acetylation on lactating mammary gland ( Lact , blue) and liver tissue ( Lvr , red). Sites investigated are indicated by rectangles: filled rectangles, H3AC enriched; open rectangle, non-enriched (See figure S3 ). ( DHS ) Summary of <t>DNaseI</t> Hypersensitivity analysis in MEC isolated from from virgin mammary glands at 12 weeks of age, staged at diestrus ( Virg , black), in lactating mammary gland ( Lact , blue) and liver tissue ( Lvr , red). Sites investigated are indicated by rectangles: filled rectangle, DHS; open rectangle, non-DHS (see Fig. S4 ). ( DNAme ) Summary of results of <t>DNA</t> methylation analysis on lactating mammary gland ( Lact ) and liver tissue ( Lvr ) (see also Fig. 5). Levels of DNA methylation are based on Fig. 5A and Table S2 and are color coded as follows: 0–20% lightest blue, 21–40% light blue, 41–60% blue, 61–80% dark blue and 80–100% black; open rectangles: not analyzed. ( K9me2 ) Summary of ChIP-chip for H3K9me2, a histone modification associated with closed chromatin, on liver tissue from [62] ; red filled rectangle indicates enrichment of H3K9me2 in liver tissue.
    Dnasei, supplied by Promega, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dnasei/product/Promega
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dnasei - by Bioz Stars, 2021-04
    97/100 stars
      Buy from Supplier

    Image Search Results


    Chromatin markers in the casein gene cluster region in different tissues and developmental stages. This illustration is a close-up of the CSN region from Figure 1 . ( A ) ChIP-seq reads for H3K4me2 in MEC isolated from virgin mammary glands at 12 weeks of age, staged at diestrus ( Virgin MG , black), in lactating mammary glands ( Lactating MG , blue) and in liver tissue ( Liver , red). A summary of peaks identified by MAC and SICER are at the top of each of the Virgin MG, Lactating MG, and Liver panels. In the evolutionary conserved region ( ECR ) panel, ECRs are shown in green; ECR1, 6 16 and 21 are indicated in pink. In the bottom panel, the locations of the casein genes (black) and non-casein genes (gray) are indicated by solid arrows denoting the direction of transcription. ( B ) Summary of markers of epigenetic regulation in the CSN region. ( H3Ac ) Summary of results of ChIP for H3-acetylation on lactating mammary gland ( Lact , blue) and liver tissue ( Lvr , red). Sites investigated are indicated by rectangles: filled rectangles, H3AC enriched; open rectangle, non-enriched (See figure S3 ). ( DHS ) Summary of DNaseI Hypersensitivity analysis in MEC isolated from from virgin mammary glands at 12 weeks of age, staged at diestrus ( Virg , black), in lactating mammary gland ( Lact , blue) and liver tissue ( Lvr , red). Sites investigated are indicated by rectangles: filled rectangle, DHS; open rectangle, non-DHS (see Fig. S4 ). ( DNAme ) Summary of results of DNA methylation analysis on lactating mammary gland ( Lact ) and liver tissue ( Lvr ) (see also Fig. 5). Levels of DNA methylation are based on Fig. 5A and Table S2 and are color coded as follows: 0–20% lightest blue, 21–40% light blue, 41–60% blue, 61–80% dark blue and 80–100% black; open rectangles: not analyzed. ( K9me2 ) Summary of ChIP-chip for H3K9me2, a histone modification associated with closed chromatin, on liver tissue from [62] ; red filled rectangle indicates enrichment of H3K9me2 in liver tissue.

    Journal: PLoS ONE

    Article Title: Epigenetic Modifications Unlock the Milk Protein Gene Loci during Mouse Mammary Gland Development and Differentiation

    doi: 10.1371/journal.pone.0053270

    Figure Lengend Snippet: Chromatin markers in the casein gene cluster region in different tissues and developmental stages. This illustration is a close-up of the CSN region from Figure 1 . ( A ) ChIP-seq reads for H3K4me2 in MEC isolated from virgin mammary glands at 12 weeks of age, staged at diestrus ( Virgin MG , black), in lactating mammary glands ( Lactating MG , blue) and in liver tissue ( Liver , red). A summary of peaks identified by MAC and SICER are at the top of each of the Virgin MG, Lactating MG, and Liver panels. In the evolutionary conserved region ( ECR ) panel, ECRs are shown in green; ECR1, 6 16 and 21 are indicated in pink. In the bottom panel, the locations of the casein genes (black) and non-casein genes (gray) are indicated by solid arrows denoting the direction of transcription. ( B ) Summary of markers of epigenetic regulation in the CSN region. ( H3Ac ) Summary of results of ChIP for H3-acetylation on lactating mammary gland ( Lact , blue) and liver tissue ( Lvr , red). Sites investigated are indicated by rectangles: filled rectangles, H3AC enriched; open rectangle, non-enriched (See figure S3 ). ( DHS ) Summary of DNaseI Hypersensitivity analysis in MEC isolated from from virgin mammary glands at 12 weeks of age, staged at diestrus ( Virg , black), in lactating mammary gland ( Lact , blue) and liver tissue ( Lvr , red). Sites investigated are indicated by rectangles: filled rectangle, DHS; open rectangle, non-DHS (see Fig. S4 ). ( DNAme ) Summary of results of DNA methylation analysis on lactating mammary gland ( Lact ) and liver tissue ( Lvr ) (see also Fig. 5). Levels of DNA methylation are based on Fig. 5A and Table S2 and are color coded as follows: 0–20% lightest blue, 21–40% light blue, 41–60% blue, 61–80% dark blue and 80–100% black; open rectangles: not analyzed. ( K9me2 ) Summary of ChIP-chip for H3K9me2, a histone modification associated with closed chromatin, on liver tissue from [62] ; red filled rectangle indicates enrichment of H3K9me2 in liver tissue.

    Article Snippet: Nuclei were then collected by centrifugation and resuspended in A+/NT (300 mM Sucrose, 60 mM KCl, 15 mM NaCl, 2 mM EDTA, 2 mM EGTA, 0.1%TritonX-100, 10 mM Tris-HCl pH 7.9, 0.15 mM Spermidine, 1 mM Spermine, 14 mM beta-Mercaptoethanol, 0.1 mM PMSF, 1 mM DTT), Nuclei were collected by centrifugation and digested in Nuclear Digestion Buffer buffer (NDB: Sucrose 300 mM, 60 mM KCl, 15 mM NaCL, 2 mM EGTA, 2 mM EDTA, 15 mM Tris pH 7.4, 5 mMMgCl2 , 0.1 mM PMSF, 0.5 mM DTT) with MgCl2 and CaCl2 added to 0.4 mM and 2 mM respectively at a standard DNA: DNaseI (RQ1 RNase-Free DNase, Promega) concentration for increasing time.

    Techniques: Chromatin Immunoprecipitation, Isolation, DNA Methylation Assay, Modification

    Analysis of host factor recruitment to HSV1 capsids. Partially tegumented viral capsids were generated from mature extracellular particles released from HSV1 infected cells by detergent lysis to remove the viral envelope in the presence of 0.1 M, 0.5 M or 1 M KCl to extract different amounts of tegument (light green), and purified through sucrose cushions. Untegumented nuclear B and C capsids (dark green) were isolated from the nuclei of HSV1 infected cells by gradient sedimentation. The 5 different capsid types were resuspended in BRB80 buffer using tip sonification, treated with DNase and RNase, repelleted, and then incubated with cytosolic extracts or purified MAPs (colored circles). Capsids were analyzed for bound host factors either after sedimentation through a sucrose cushion by immunoblot or directly by electron microscopy after immunolabeling and negative contrasting.

    Journal: PLoS Pathogens

    Article Title: Plus- and Minus-End Directed Microtubule Motors Bind Simultaneously to Herpes Simplex Virus Capsids Using Different Inner Tegument Structures

    doi: 10.1371/journal.ppat.1000991

    Figure Lengend Snippet: Analysis of host factor recruitment to HSV1 capsids. Partially tegumented viral capsids were generated from mature extracellular particles released from HSV1 infected cells by detergent lysis to remove the viral envelope in the presence of 0.1 M, 0.5 M or 1 M KCl to extract different amounts of tegument (light green), and purified through sucrose cushions. Untegumented nuclear B and C capsids (dark green) were isolated from the nuclei of HSV1 infected cells by gradient sedimentation. The 5 different capsid types were resuspended in BRB80 buffer using tip sonification, treated with DNase and RNase, repelleted, and then incubated with cytosolic extracts or purified MAPs (colored circles). Capsids were analyzed for bound host factors either after sedimentation through a sucrose cushion by immunoblot or directly by electron microscopy after immunolabeling and negative contrasting.

    Article Snippet: The pellets were resuspended in BRB80 buffer with 10 mM DTT, 1 mg/ml soybean trypsin inhibitor, protease inhibitors, 100 µg/ml RNase (Roth, Germany) and 0.1 U/µl DNase I (M6101, Promega, USA) and treated as described for viral capsids.

    Techniques: Generated, Infection, Lysis, Purification, Isolation, Sedimentation, Incubation, Electron Microscopy, Immunolabeling