rnase free dnase  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90

    Structured Review

    Thermo Fisher rnase free dnase
    Rnase Free Dnase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 350 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rnase free dnase/product/Thermo Fisher
    Average 90 stars, based on 350 article reviews
    Price from $9.99 to $1999.99
    rnase free dnase - by Bioz Stars, 2020-04
    90/100 stars

    Images

    Related Articles

    Centrifugation:

    Article Title: Evaluation of Lsa46 and Lsa77 Leptospiral Proteins for Their Immunoprotective Activities in Hamster Model of Leptospirosis
    Article Snippet: .. Leptospiral RNA Extraction and cDNA Generation Leptospiral cells at late-log phase contained in 30 mL EMJH medium were collected by centrifugation (8,000 x g for 15 min) in RNase-free tubes and resulting pellet was resuspended in 1 mL Trizol reagent (Invitrogen), vortexed, and incubated for 10 min at room temperature. .. Samples were centrifuged at 12,000 x g for 15 min at 4°C for phase separation; the aqueous phase was transferred to a new tube and 660 μ L of isopropanol was added for nucleic acid precipitation, performed at room temperature for 10 min. Then, tubes were centrifuged (12,000 x g for 10 min, 4°C) and the resulting pellet was washed with 1.35 mL of 75% ethanol, diluted in RNase-free water, and recovered by centrifugation at 7,500 x g for 5 min at 4°C.

    Article Title: An RNA-seq Protocol to Identify mRNA Expression Changes in Mouse Diaphyseal Bone: Applications in Mice with Bone Property Altering Lrp5 Mutations
    Article Snippet: The distal and proximal epiphyses were excised and the diaphyseal bone marrow was removed by centrifugation at > 15,000 x g for 1 min at room temperature. .. RNA was recovered from each tibia sample by pulverizing the bone inside 2ml RNase-free microtubes with metal beads and 1 ml TRIzol (Life Technologies, Grand Island, NY) using a benchtop tissue homogenizer (FastPrep24, MP Biomedicals, Solon, OH).

    Positive Control:

    Article Title: PCR-Independent Detection of Bacterial Species-Specific 16S rRNA at 10 fM by a Pore-Blockage Sensor
    Article Snippet: For positive control experiments, the universal PNA probe NH2 -(CH2 CH2 O)12 - CTG CCT CCC GTA GGA was used [ ]. .. PureLink RNA Mini Kit, PureLink DNase Set, RNase free water, RNase-free pipette tips, RNase-away reagent and RNase-free microfuge tubes were purchased from Invitrogen Life Technologies (Grand Island, NY, USA).

    Synthesized:

    Article Title: Potential Role of Mesenchymal Stem Cells in Alleviating Intestinal Ischemia/Reperfusion Impairment
    Article Snippet: .. Real-time PCR for determining proliferating cell nuclear antigen (PCNA) expression in intestinal mucosa 0.1g ileum mucosa of each group was collected in RNase-free tubes and total RNAs of intestinal mucosal tissue was extracted using Trizol reagents (Invitrogen Company, US). cDNA was synthesized using the prime ScriptTM 1st strand cDNA synthesis kit (Takara, Japan) according to the manufacture’s protocol. ..

    Article Title: Sleep Disruption Aggravates Focal Cerebral Ischemia in the Rat
    Article Snippet: Brain tissues collected in RNase-free tubes (see above) were subjected to RNA extraction by the Trizol method (Life Technologies, Rockville, MD, USA) for individual animals. .. Oligo(dT)15 primed the first-strand cDNA synthesis was synthesized by AMV reverse transcriptase (Promega).

    Quantitative RT-PCR:

    Article Title: Burn injury-induced IRS-1 degradation in mouse skeletal muscle
    Article Snippet: Paragraph title: Real-time RT-PCR analysis ... Frozen tissue samples (~50 mg) were cut into small pieces in RNase-free tubes, and homogenized with 5 strokes (5 seconds each) with an Omni TH-tissue homogenizer in sterilized eppendorf tubes (2 ml) containing TRI reagent solution (cat# AM9738, 1 ml), Applied Biosystem, Framingham, MA, USA).

    Electrophoresis:

    Article Title: Gender Differences of B Cell Signature in Healthy Subjects Underlie Disparities in Incidence and Course of SLE Related to Estrogen
    Article Snippet: RNA Isolation and Microarray The purified B cells were centrifuged in RNase-free tubes treated with Trizol reagent (Invitrogen, USA). .. RNA quality was determined by formaldehyde denaturation electrophoresis and only those samples with a 260 nm/280 nm optical density ratio (OD260/280) > 1.8 and a total RNA concentration > 1 mg/mL were submitted for hybridization to generate labeled targets.

    Microarray:

    Article Title: Gender Differences of B Cell Signature in Healthy Subjects Underlie Disparities in Incidence and Course of SLE Related to Estrogen
    Article Snippet: .. RNA Isolation and Microarray The purified B cells were centrifuged in RNase-free tubes treated with Trizol reagent (Invitrogen, USA). .. Total RNA was extracted by using the RNeasy kit according to the instructions of the manufacture (Qiagen, Valencia, CA) and then RNA concentration was assessed by Nanodrop 2000 spectrophotometry (Thermo).

    Incubation:

    Article Title: Potential Role of Mesenchymal Stem Cells in Alleviating Intestinal Ischemia/Reperfusion Impairment
    Article Snippet: Real-time PCR for determining proliferating cell nuclear antigen (PCNA) expression in intestinal mucosa 0.1g ileum mucosa of each group was collected in RNase-free tubes and total RNAs of intestinal mucosal tissue was extracted using Trizol reagents (Invitrogen Company, US). cDNA was synthesized using the prime ScriptTM 1st strand cDNA synthesis kit (Takara, Japan) according to the manufacture’s protocol. .. The supernatants were transferred to new RNase-free tubes and mixed with isopropanol at a ratio of 1:1, then centrifuged at 14,000 g (4°C) for 15 mins after incubation for 10 mins.

    Article Title: Evaluation of Lsa46 and Lsa77 Leptospiral Proteins for Their Immunoprotective Activities in Hamster Model of Leptospirosis
    Article Snippet: .. Leptospiral RNA Extraction and cDNA Generation Leptospiral cells at late-log phase contained in 30 mL EMJH medium were collected by centrifugation (8,000 x g for 15 min) in RNase-free tubes and resulting pellet was resuspended in 1 mL Trizol reagent (Invitrogen), vortexed, and incubated for 10 min at room temperature. .. Samples were centrifuged at 12,000 x g for 15 min at 4°C for phase separation; the aqueous phase was transferred to a new tube and 660 μ L of isopropanol was added for nucleic acid precipitation, performed at room temperature for 10 min. Then, tubes were centrifuged (12,000 x g for 10 min, 4°C) and the resulting pellet was washed with 1.35 mL of 75% ethanol, diluted in RNase-free water, and recovered by centrifugation at 7,500 x g for 5 min at 4°C.

    Article Title: Gene Expression Analysis of Human Islets in a Subject at Onset of Type 1 Diabetes
    Article Snippet: Islets were identified and cut under 10× magnification and collected in RNAse free microfuge tubes containing 50 mL of Arcturus® PicoPure® Extraction Buffer, (Applied Biosystems, Sweden). .. After laser capturing the tissue was incubated at 42°C for 30 minutes in the extraction buffer.

    Article Title: Maintaining RNA Integrity for Transcriptomic Profiling of Ex Vivo Cultured Limbal Epithelial Stem Cells after Fluorescence-Activated Cell Sorting (FACS)
    Article Snippet: After scraping off the endothelial side, the rings were incubated with 2.4 units/ml dispase II (Life Technologies) in sPBS at 37 °C for 1 h. Cells were then removed from the epithelial side, pooled briefly and pelleted at 300 g for 5 min, incubated with TrypLE Express (Life Technologies) at 37 °C for 8 min, and finally purified through a 70 μm cell-strainer (BD Biosciences, San Jose, CA). .. Single cell suspensions of 0.5 to 1.5 × 106 cells were aliquoted into 2.0 ml RNase-free microfuge tubes (Ambion, Naerum, Denmark) for subsequent immunofluorescence staining.

    Cell Culture:

    Article Title: Maintaining RNA Integrity for Transcriptomic Profiling of Ex Vivo Cultured Limbal Epithelial Stem Cells after Fluorescence-Activated Cell Sorting (FACS)
    Article Snippet: Paragraph title: Cell Culture ... Single cell suspensions of 0.5 to 1.5 × 106 cells were aliquoted into 2.0 ml RNase-free microfuge tubes (Ambion, Naerum, Denmark) for subsequent immunofluorescence staining.

    Expressing:

    Article Title: Potential Role of Mesenchymal Stem Cells in Alleviating Intestinal Ischemia/Reperfusion Impairment
    Article Snippet: .. Real-time PCR for determining proliferating cell nuclear antigen (PCNA) expression in intestinal mucosa 0.1g ileum mucosa of each group was collected in RNase-free tubes and total RNAs of intestinal mucosal tissue was extracted using Trizol reagents (Invitrogen Company, US). cDNA was synthesized using the prime ScriptTM 1st strand cDNA synthesis kit (Takara, Japan) according to the manufacture’s protocol. ..

    Article Title: The relationship between the expression levels of miR-135a and HOXA10 gene in the eutopic and ectopic endometrium
    Article Snippet: The tissues were stored in RNase free microtubes containing RNAlater™ Stabilization Solution (Thermo Fisher Scientific) at -80oC to maintain their RNA stability. .. RNA Isolation and quantitative real time-PCR In order to examine the expression rate of the HOXA10 gene and miR-135a in the tissues of patients and healthy subjects, the total RNA was first extracted from the tissues using Trizol Kit (Invitrogen, USA).

    Article Title: Sleep Disruption Aggravates Focal Cerebral Ischemia in the Rat
    Article Snippet: Paragraph title: Taqman Gene Expression Assay ... Brain tissues collected in RNase-free tubes (see above) were subjected to RNA extraction by the Trizol method (Life Technologies, Rockville, MD, USA) for individual animals.

    Article Title: MeCP2 interacts with chromosomal microRNAs in brain
    Article Snippet: Protein expression was visualized using enhanced chemiluminescent reagent kit (Sigma-Aldrich) and detected by exposing membranes to high performance chemiluminescent film for an optimized time. .. To avoid RNA degradation while performing RIP, RNase free tubes and RNase inhibitor RNaseOUT reagent (Invitrogen) were used.

    Hybridization:

    Article Title: Gender Differences of B Cell Signature in Healthy Subjects Underlie Disparities in Incidence and Course of SLE Related to Estrogen
    Article Snippet: RNA Isolation and Microarray The purified B cells were centrifuged in RNase-free tubes treated with Trizol reagent (Invitrogen, USA). .. RNA quality was determined by formaldehyde denaturation electrophoresis and only those samples with a 260 nm/280 nm optical density ratio (OD260/280) > 1.8 and a total RNA concentration > 1 mg/mL were submitted for hybridization to generate labeled targets.

    Countercurrent Chromatography:

    Article Title: PCR-Independent Detection of Bacterial Species-Specific 16S rRNA at 10 fM by a Pore-Blockage Sensor
    Article Snippet: For positive control experiments, the universal PNA probe NH2 -(CH2 CH2 O)12 - CTG CCT CCC GTA GGA was used [ ]. .. PureLink RNA Mini Kit, PureLink DNase Set, RNase free water, RNase-free pipette tips, RNase-away reagent and RNase-free microfuge tubes were purchased from Invitrogen Life Technologies (Grand Island, NY, USA).

    Immunoprecipitation:

    Article Title: MeCP2 interacts with chromosomal microRNAs in brain
    Article Snippet: Paragraph title: RNA immunoprecipitation and massive parallel sequencing (RIP-seq) ... To avoid RNA degradation while performing RIP, RNase free tubes and RNase inhibitor RNaseOUT reagent (Invitrogen) were used.

    Transferring:

    Article Title: PCR-Independent Detection of Bacterial Species-Specific 16S rRNA at 10 fM by a Pore-Blockage Sensor
    Article Snippet: .. PureLink RNA Mini Kit, PureLink DNase Set, RNase free water, RNase-free pipette tips, RNase-away reagent and RNase-free microfuge tubes were purchased from Invitrogen Life Technologies (Grand Island, NY, USA). ..

    Northern Blot:

    Article Title: The role of DNA methylation in human trophoblast differentiation
    Article Snippet: Use of first trimester placental tissue in this study was approved by the Northern Regional Ethics Committee (NTX/12/06/057/AM04). .. Live cell populations were sorted directly into 200 µL of sterile PBS in 2 mL RNAse-free Eppendorf tubes for DNA extraction (n = 4 placentae), or into 200 µL of RNA Later® (Cat no. AM7021, Ambion) for RNA (n = 5 placentae) or miRNA (n = 4 placentae) extraction.

    Infection:

    Article Title: The Mature Virion of Ectromelia Virus, a Pathogenic Poxvirus, Is Capable of Intrahepatic Spread and Can Serve as a Target for Delayed Therapy
    Article Snippet: .. D-LNs were collected from infected or naive mice and immediately placed in RNase-free tubes containing RNAlater (Ambion). .. RNA was extracted using an RNeasy kit (Qiagen), as described by the manufacturer, and the DNA was digested during the process with DNase (Qiagen).

    Sequencing:

    Article Title: MeCP2 interacts with chromosomal microRNAs in brain
    Article Snippet: Paragraph title: RNA immunoprecipitation and massive parallel sequencing (RIP-seq) ... To avoid RNA degradation while performing RIP, RNase free tubes and RNase inhibitor RNaseOUT reagent (Invitrogen) were used.

    Immunofluorescence:

    Article Title: Maintaining RNA Integrity for Transcriptomic Profiling of Ex Vivo Cultured Limbal Epithelial Stem Cells after Fluorescence-Activated Cell Sorting (FACS)
    Article Snippet: .. Single cell suspensions of 0.5 to 1.5 × 106 cells were aliquoted into 2.0 ml RNase-free microfuge tubes (Ambion, Naerum, Denmark) for subsequent immunofluorescence staining. .. The isolation and expansion process were repeated at 3 distinct time points.

    DNA Extraction:

    Article Title: The role of DNA methylation in human trophoblast differentiation
    Article Snippet: .. Live cell populations were sorted directly into 200 µL of sterile PBS in 2 mL RNAse-free Eppendorf tubes for DNA extraction (n = 4 placentae), or into 200 µL of RNA Later® (Cat no. AM7021, Ambion) for RNA (n = 5 placentae) or miRNA (n = 4 placentae) extraction. ..

    Isolation:

    Article Title: The relationship between the expression levels of miR-135a and HOXA10 gene in the eutopic and ectopic endometrium
    Article Snippet: The tissues were stored in RNase free microtubes containing RNAlater™ Stabilization Solution (Thermo Fisher Scientific) at -80oC to maintain their RNA stability. .. RNA Isolation and quantitative real time-PCR In order to examine the expression rate of the HOXA10 gene and miR-135a in the tissues of patients and healthy subjects, the total RNA was first extracted from the tissues using Trizol Kit (Invitrogen, USA).

    Article Title: The Mature Virion of Ectromelia Virus, a Pathogenic Poxvirus, Is Capable of Intrahepatic Spread and Can Serve as a Target for Delayed Therapy
    Article Snippet: Paragraph title: RNA isolation. ... D-LNs were collected from infected or naive mice and immediately placed in RNase-free tubes containing RNAlater (Ambion).

    Article Title: Galanin Transgenic Mice with Elevated Circulating Galanin Levels Alleviate Demyelination in a Cuprizone-Induced MS Mouse Model
    Article Snippet: .. Isolated tissues were quickly transferred into 2.0 ml RNase-free microtubes containing 0.6 ml of ice-cold TRIzol (Cat # 15596026, Invitrogen). .. Total RNA was extracted from each homogenized tissue sample (processed using Homogenizer Power Gen 125, Generator Flat 5×95, Fisher Scientific) using the phenol/chloroform extraction protocol provided by Invitrogen (TRIzol Reagent).

    Article Title: Sleep Disruption Aggravates Focal Cerebral Ischemia in the Rat
    Article Snippet: Brain tissues collected in RNase-free tubes (see above) were subjected to RNA extraction by the Trizol method (Life Technologies, Rockville, MD, USA) for individual animals. .. Group cDNA samples were synthesized by pooling together the RNA isolated from each animal.

    Article Title: The role of DNA methylation in human trophoblast differentiation
    Article Snippet: Paragraph title: Isolation of trophoblast populations ... Live cell populations were sorted directly into 200 µL of sterile PBS in 2 mL RNAse-free Eppendorf tubes for DNA extraction (n = 4 placentae), or into 200 µL of RNA Later® (Cat no. AM7021, Ambion) for RNA (n = 5 placentae) or miRNA (n = 4 placentae) extraction.

    Article Title: Burn injury-induced IRS-1 degradation in mouse skeletal muscle
    Article Snippet: Frozen tissue samples (~50 mg) were cut into small pieces in RNase-free tubes, and homogenized with 5 strokes (5 seconds each) with an Omni TH-tissue homogenizer in sterilized eppendorf tubes (2 ml) containing TRI reagent solution (cat# AM9738, 1 ml), Applied Biosystem, Framingham, MA, USA). .. The quality of the isolated RNA was assessed with agarose gel electrophoresis.

    Article Title: Gender Differences of B Cell Signature in Healthy Subjects Underlie Disparities in Incidence and Course of SLE Related to Estrogen
    Article Snippet: .. RNA Isolation and Microarray The purified B cells were centrifuged in RNase-free tubes treated with Trizol reagent (Invitrogen, USA). .. Total RNA was extracted by using the RNeasy kit according to the instructions of the manufacture (Qiagen, Valencia, CA) and then RNA concentration was assessed by Nanodrop 2000 spectrophotometry (Thermo).

    Article Title: Sexually Dimorphic MicroRNA Expression During Chicken Embryonic Gonadal Development 1
    Article Snippet: All RNA extractions were carried out using the mirVana miRNA Isolation Kit (Ambion), using the protocol for fractionation and enrichment for small RNAs. .. Gonad lysates from E5.5 and E6.5 embryos were pooled according to sex, in 2-ml RNase-free tubes (Ambion) prior to RNA extraction.

    Labeling:

    Article Title: Gender Differences of B Cell Signature in Healthy Subjects Underlie Disparities in Incidence and Course of SLE Related to Estrogen
    Article Snippet: RNA Isolation and Microarray The purified B cells were centrifuged in RNase-free tubes treated with Trizol reagent (Invitrogen, USA). .. RNA quality was determined by formaldehyde denaturation electrophoresis and only those samples with a 260 nm/280 nm optical density ratio (OD260/280) > 1.8 and a total RNA concentration > 1 mg/mL were submitted for hybridization to generate labeled targets.

    Mouse Assay:

    Article Title: The Mature Virion of Ectromelia Virus, a Pathogenic Poxvirus, Is Capable of Intrahepatic Spread and Can Serve as a Target for Delayed Therapy
    Article Snippet: .. D-LNs were collected from infected or naive mice and immediately placed in RNase-free tubes containing RNAlater (Ambion). .. RNA was extracted using an RNeasy kit (Qiagen), as described by the manufacturer, and the DNA was digested during the process with DNase (Qiagen).

    Article Title: Galanin Transgenic Mice with Elevated Circulating Galanin Levels Alleviate Demyelination in a Cuprizone-Induced MS Mouse Model
    Article Snippet: Mice were anesthetized by isoflurane inhalation and were quickly decapitated. .. Isolated tissues were quickly transferred into 2.0 ml RNase-free microtubes containing 0.6 ml of ice-cold TRIzol (Cat # 15596026, Invitrogen).

    Purification:

    Article Title: Galanin Transgenic Mice with Elevated Circulating Galanin Levels Alleviate Demyelination in a Cuprizone-Induced MS Mouse Model
    Article Snippet: Isolated tissues were quickly transferred into 2.0 ml RNase-free microtubes containing 0.6 ml of ice-cold TRIzol (Cat # 15596026, Invitrogen). .. Subsequently, the total RNA was purified using the RNeasy Mini Kit (Cat # 74004, QIAGEN) according to the manufacturer's protocol; DNA-decontamination treatment was included.

    Article Title: MeCP2 interacts with chromosomal microRNAs in brain
    Article Snippet: To avoid RNA degradation while performing RIP, RNase free tubes and RNase inhibitor RNaseOUT reagent (Invitrogen) were used. .. RNA was purified from immunopurified chromatin and sequenced with massive parallel sequencing for the discovery of small ncRNAs bound by MeCP2.

    Article Title: Gender Differences of B Cell Signature in Healthy Subjects Underlie Disparities in Incidence and Course of SLE Related to Estrogen
    Article Snippet: .. RNA Isolation and Microarray The purified B cells were centrifuged in RNase-free tubes treated with Trizol reagent (Invitrogen, USA). .. Total RNA was extracted by using the RNeasy kit according to the instructions of the manufacture (Qiagen, Valencia, CA) and then RNA concentration was assessed by Nanodrop 2000 spectrophotometry (Thermo).

    Article Title: An RNA-seq Protocol to Identify mRNA Expression Changes in Mouse Diaphyseal Bone: Applications in Mice with Bone Property Altering Lrp5 Mutations
    Article Snippet: RNA was recovered from each tibia sample by pulverizing the bone inside 2ml RNase-free microtubes with metal beads and 1 ml TRIzol (Life Technologies, Grand Island, NY) using a benchtop tissue homogenizer (FastPrep24, MP Biomedicals, Solon, OH). .. The RNA containing fraction (400 μl per sample) was then purified (Purelink RNA Mini Kit, Life Technologies, Grand Island, NY) and treated with DNase I (RNase-Free DNase Set, Qiagen, Valencia, CA) for 15 minutes.

    Article Title: Sexually Dimorphic MicroRNA Expression During Chicken Embryonic Gonadal Development 1
    Article Snippet: Paragraph title: MicroRNA Extraction and Purification ... Gonad lysates from E5.5 and E6.5 embryos were pooled according to sex, in 2-ml RNase-free tubes (Ambion) prior to RNA extraction.

    Article Title: Maintaining RNA Integrity for Transcriptomic Profiling of Ex Vivo Cultured Limbal Epithelial Stem Cells after Fluorescence-Activated Cell Sorting (FACS)
    Article Snippet: After scraping off the endothelial side, the rings were incubated with 2.4 units/ml dispase II (Life Technologies) in sPBS at 37 °C for 1 h. Cells were then removed from the epithelial side, pooled briefly and pelleted at 300 g for 5 min, incubated with TrypLE Express (Life Technologies) at 37 °C for 8 min, and finally purified through a 70 μm cell-strainer (BD Biosciences, San Jose, CA). .. Single cell suspensions of 0.5 to 1.5 × 106 cells were aliquoted into 2.0 ml RNase-free microfuge tubes (Ambion, Naerum, Denmark) for subsequent immunofluorescence staining.

    Chromatin Immunoprecipitation:

    Article Title: MeCP2 interacts with chromosomal microRNAs in brain
    Article Snippet: Chromatin immunoprecipitation was performed on MNase digested chromatin fractions S1 and S2 using anti-MeCP2 antibody. .. To avoid RNA degradation while performing RIP, RNase free tubes and RNase inhibitor RNaseOUT reagent (Invitrogen) were used.

    Software:

    Article Title: Gene Expression Analysis of Human Islets in a Subject at Onset of Type 1 Diabetes
    Article Snippet: For selection and extraction of specimen, laser microdissection was performed, essentially as described [ ], using a Leica LMD 6000, a Leica DFC300 FX camera and Leica Laser Microdissection v 7.4 software. .. Islets were identified and cut under 10× magnification and collected in RNAse free microfuge tubes containing 50 mL of Arcturus® PicoPure® Extraction Buffer, (Applied Biosystems, Sweden).

    Real-time Polymerase Chain Reaction:

    Article Title: Potential Role of Mesenchymal Stem Cells in Alleviating Intestinal Ischemia/Reperfusion Impairment
    Article Snippet: .. Real-time PCR for determining proliferating cell nuclear antigen (PCNA) expression in intestinal mucosa 0.1g ileum mucosa of each group was collected in RNase-free tubes and total RNAs of intestinal mucosal tissue was extracted using Trizol reagents (Invitrogen Company, US). cDNA was synthesized using the prime ScriptTM 1st strand cDNA synthesis kit (Takara, Japan) according to the manufacture’s protocol. ..

    Article Title: The relationship between the expression levels of miR-135a and HOXA10 gene in the eutopic and ectopic endometrium
    Article Snippet: The tissues were stored in RNase free microtubes containing RNAlater™ Stabilization Solution (Thermo Fisher Scientific) at -80oC to maintain their RNA stability. .. RNA Isolation and quantitative real time-PCR In order to examine the expression rate of the HOXA10 gene and miR-135a in the tissues of patients and healthy subjects, the total RNA was first extracted from the tissues using Trizol Kit (Invitrogen, USA).

    RNA Extraction:

    Article Title: Sleep Disruption Aggravates Focal Cerebral Ischemia in the Rat
    Article Snippet: .. Brain tissues collected in RNase-free tubes (see above) were subjected to RNA extraction by the Trizol method (Life Technologies, Rockville, MD, USA) for individual animals. .. The total RNA was treated with RQ1 DNase (Promega, Madison, WI, USA) to digest genomic DNA.

    Article Title: Burn injury-induced IRS-1 degradation in mouse skeletal muscle
    Article Snippet: Frozen tissue samples (~50 mg) were cut into small pieces in RNase-free tubes, and homogenized with 5 strokes (5 seconds each) with an Omni TH-tissue homogenizer in sterilized eppendorf tubes (2 ml) containing TRI reagent solution (cat# AM9738, 1 ml), Applied Biosystem, Framingham, MA, USA). .. Frozen tissue samples (~50 mg) were cut into small pieces in RNase-free tubes, and homogenized with 5 strokes (5 seconds each) with an Omni TH-tissue homogenizer in sterilized eppendorf tubes (2 ml) containing TRI reagent solution (cat# AM9738, 1 ml), Applied Biosystem, Framingham, MA, USA).

    Article Title: Evaluation of Lsa46 and Lsa77 Leptospiral Proteins for Their Immunoprotective Activities in Hamster Model of Leptospirosis
    Article Snippet: .. Leptospiral RNA Extraction and cDNA Generation Leptospiral cells at late-log phase contained in 30 mL EMJH medium were collected by centrifugation (8,000 x g for 15 min) in RNase-free tubes and resulting pellet was resuspended in 1 mL Trizol reagent (Invitrogen), vortexed, and incubated for 10 min at room temperature. .. Samples were centrifuged at 12,000 x g for 15 min at 4°C for phase separation; the aqueous phase was transferred to a new tube and 660 μ L of isopropanol was added for nucleic acid precipitation, performed at room temperature for 10 min. Then, tubes were centrifuged (12,000 x g for 10 min, 4°C) and the resulting pellet was washed with 1.35 mL of 75% ethanol, diluted in RNase-free water, and recovered by centrifugation at 7,500 x g for 5 min at 4°C.

    Article Title: Sexually Dimorphic MicroRNA Expression During Chicken Embryonic Gonadal Development 1
    Article Snippet: .. Gonad lysates from E5.5 and E6.5 embryos were pooled according to sex, in 2-ml RNase-free tubes (Ambion) prior to RNA extraction. .. Both small RNA (miRNA, < 200 nucleotides) and high-molecular weight fractions were collected for each sample and eluted in 100 μl of nuclease-free water (NF-H2 O) (Ambion) preheated to 95°C.

    Selection:

    Article Title: Gene Expression Analysis of Human Islets in a Subject at Onset of Type 1 Diabetes
    Article Snippet: For selection and extraction of specimen, laser microdissection was performed, essentially as described [ ], using a Leica LMD 6000, a Leica DFC300 FX camera and Leica Laser Microdissection v 7.4 software. .. Islets were identified and cut under 10× magnification and collected in RNAse free microfuge tubes containing 50 mL of Arcturus® PicoPure® Extraction Buffer, (Applied Biosystems, Sweden).

    Agarose Gel Electrophoresis:

    Article Title: Burn injury-induced IRS-1 degradation in mouse skeletal muscle
    Article Snippet: Frozen tissue samples (~50 mg) were cut into small pieces in RNase-free tubes, and homogenized with 5 strokes (5 seconds each) with an Omni TH-tissue homogenizer in sterilized eppendorf tubes (2 ml) containing TRI reagent solution (cat# AM9738, 1 ml), Applied Biosystem, Framingham, MA, USA). .. The quality of the isolated RNA was assessed with agarose gel electrophoresis.

    Laser Capture Microdissection:

    Article Title: Gene Expression Analysis of Human Islets in a Subject at Onset of Type 1 Diabetes
    Article Snippet: Paragraph title: Laser capture microdissection ... Islets were identified and cut under 10× magnification and collected in RNAse free microfuge tubes containing 50 mL of Arcturus® PicoPure® Extraction Buffer, (Applied Biosystems, Sweden).

    Spectrophotometry:

    Article Title: Gender Differences of B Cell Signature in Healthy Subjects Underlie Disparities in Incidence and Course of SLE Related to Estrogen
    Article Snippet: RNA Isolation and Microarray The purified B cells were centrifuged in RNase-free tubes treated with Trizol reagent (Invitrogen, USA). .. Total RNA was extracted by using the RNeasy kit according to the instructions of the manufacture (Qiagen, Valencia, CA) and then RNA concentration was assessed by Nanodrop 2000 spectrophotometry (Thermo).

    Article Title: Sexually Dimorphic MicroRNA Expression During Chicken Embryonic Gonadal Development 1
    Article Snippet: Gonad lysates from E5.5 and E6.5 embryos were pooled according to sex, in 2-ml RNase-free tubes (Ambion) prior to RNA extraction. .. RNA concentrations were determined using a NanoDrop 1000 spectrophotometer (Thermo Scientific).

    Concentration Assay:

    Article Title: Gender Differences of B Cell Signature in Healthy Subjects Underlie Disparities in Incidence and Course of SLE Related to Estrogen
    Article Snippet: RNA Isolation and Microarray The purified B cells were centrifuged in RNase-free tubes treated with Trizol reagent (Invitrogen, USA). .. Total RNA was extracted by using the RNeasy kit according to the instructions of the manufacture (Qiagen, Valencia, CA) and then RNA concentration was assessed by Nanodrop 2000 spectrophotometry (Thermo).

    Fractionation:

    Article Title: Sexually Dimorphic MicroRNA Expression During Chicken Embryonic Gonadal Development 1
    Article Snippet: All RNA extractions were carried out using the mirVana miRNA Isolation Kit (Ambion), using the protocol for fractionation and enrichment for small RNAs. .. Gonad lysates from E5.5 and E6.5 embryos were pooled according to sex, in 2-ml RNase-free tubes (Ambion) prior to RNA extraction.

    CTG Assay:

    Article Title: PCR-Independent Detection of Bacterial Species-Specific 16S rRNA at 10 fM by a Pore-Blockage Sensor
    Article Snippet: For positive control experiments, the universal PNA probe NH2 -(CH2 CH2 O)12 - CTG CCT CCC GTA GGA was used [ ]. .. PureLink RNA Mini Kit, PureLink DNase Set, RNase free water, RNase-free pipette tips, RNase-away reagent and RNase-free microfuge tubes were purchased from Invitrogen Life Technologies (Grand Island, NY, USA).

    Staining:

    Article Title: Gene Expression Analysis of Human Islets in a Subject at Onset of Type 1 Diabetes
    Article Snippet: Hematoxylin-Eosin (HE) staining and dehydration of the tissue sections were performed with Arcturus® Histogene® Frozen Section Staining Kit (Applied Biosystems, Sweden) according to the manufacturer’s instructions. .. Islets were identified and cut under 10× magnification and collected in RNAse free microfuge tubes containing 50 mL of Arcturus® PicoPure® Extraction Buffer, (Applied Biosystems, Sweden).

    Article Title: Maintaining RNA Integrity for Transcriptomic Profiling of Ex Vivo Cultured Limbal Epithelial Stem Cells after Fluorescence-Activated Cell Sorting (FACS)
    Article Snippet: .. Single cell suspensions of 0.5 to 1.5 × 106 cells were aliquoted into 2.0 ml RNase-free microfuge tubes (Ambion, Naerum, Denmark) for subsequent immunofluorescence staining. .. The isolation and expansion process were repeated at 3 distinct time points.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Thermo Fisher rnase free
    alTERC gene is transcribed to RNA in vivo in mouse organs and in vitro in mouse cell lines A. RNA was extracted from adult mouse brain, n = 3, cDNA was generated and subjected to PCR analysis using the set 1 primers for mTERC and set 2 primers for alTERC. Two bands of ~220 bp for mTERC and ~150bp for alTERC were observed. NTC- control, no cDNA. (A is a representative picture of 3 independent experiments). B. The PCR reaction described in A was carried out in the presence of radioactive nucleotide (dCTP [αp 32 ] and the reaction products were analysed by 14% polyacrylamide gel electrophoresis following autoradiography. Two bands of ~230 bp and ~210 bp were observed with set 1 primers (Fpr1) for mTERC and one band of ~150 bp with set primers 2 (Fpr2) for alTERC were detected. C. RNA was extracted from mouse NSC-34 motor neurons like cells followed by cDNA production in the presence or absence of <t>DNase</t> or <t>RNase</t> and subjected to PCR amplification as described in A using the set1 and set 2 primers. D. RNA was extracted from mouse organs (brain and spleen) or from mouse neuroblastoma cell line (N2a) and subjected to sqPCR analysis using the set 1 and 2 primers for TERC and alTERC and GAPDH primers as control. A Representative picture of 3 independent experiments. E. The results of experiments described in D were quantified by densitometric analysis with the EZQuant software, calculated relatively to GAPDH and the alTERC/TERC expression ratio was estimated. The data are means ± SD of 3 independent experiments.
    Rnase Free, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rnase free/product/Thermo Fisher
    Average 99 stars, based on 22 article reviews
    Price from $9.99 to $1999.99
    rnase free - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher rnase a
    RNase and DNase analysis of major satellite species. Total RNA from NIH/3T3 cells was treated with indicated enzymes for 30 min at 37°, phenol:chloroform extracted, ethanol precipitated and glyoxylated prior to electrophoresis, then blotted and probed for major satellite sequences, ethidium staining of ribosomal RNA is shown as a control. A: Samples treated in water without and with RNase A. B: Samples treated in NEBuffer 3 with indicated ribonucleases. C: Samples treated in RQ1 DNase buffer without and with DNase I. For quantification, error bars represent ±1 s.d., y-axes in arbitrary units, p values calculated using a one-way ANOVA (n = 4) for B and Student’s  t -test (n = 3) for C.
    Rnase A, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1229 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rnase a/product/Thermo Fisher
    Average 99 stars, based on 1229 article reviews
    Price from $9.99 to $1999.99
    rnase a - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    94
    Thermo Fisher p5rhh
    <t>p5RHH:</t> Nox4 siRNA nanocomplex transfection downregulates Nox4 mRNA and protein expression in VSMC from 4-month old wild-type mice. (A) p5RHH: Nox4 siRNA nanocomplex transfection efficiency compared to RNAiMAX Nox4 siRNA using quantitative RT-PCR (mean ±
    P5rhh, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p5rhh/product/Thermo Fisher
    Average 94 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    p5rhh - by Bioz Stars, 2020-04
    94/100 stars
      Buy from Supplier

    Image Search Results


    alTERC gene is transcribed to RNA in vivo in mouse organs and in vitro in mouse cell lines A. RNA was extracted from adult mouse brain, n = 3, cDNA was generated and subjected to PCR analysis using the set 1 primers for mTERC and set 2 primers for alTERC. Two bands of ~220 bp for mTERC and ~150bp for alTERC were observed. NTC- control, no cDNA. (A is a representative picture of 3 independent experiments). B. The PCR reaction described in A was carried out in the presence of radioactive nucleotide (dCTP [αp 32 ] and the reaction products were analysed by 14% polyacrylamide gel electrophoresis following autoradiography. Two bands of ~230 bp and ~210 bp were observed with set 1 primers (Fpr1) for mTERC and one band of ~150 bp with set primers 2 (Fpr2) for alTERC were detected. C. RNA was extracted from mouse NSC-34 motor neurons like cells followed by cDNA production in the presence or absence of DNase or RNase and subjected to PCR amplification as described in A using the set1 and set 2 primers. D. RNA was extracted from mouse organs (brain and spleen) or from mouse neuroblastoma cell line (N2a) and subjected to sqPCR analysis using the set 1 and 2 primers for TERC and alTERC and GAPDH primers as control. A Representative picture of 3 independent experiments. E. The results of experiments described in D were quantified by densitometric analysis with the EZQuant software, calculated relatively to GAPDH and the alTERC/TERC expression ratio was estimated. The data are means ± SD of 3 independent experiments.

    Journal: Oncotarget

    Article Title: Expression of functional alternative telomerase RNA component gene in mouse brain and in motor neurons cells protects from oxidative stress

    doi: 10.18632/oncotarget.13049

    Figure Lengend Snippet: alTERC gene is transcribed to RNA in vivo in mouse organs and in vitro in mouse cell lines A. RNA was extracted from adult mouse brain, n = 3, cDNA was generated and subjected to PCR analysis using the set 1 primers for mTERC and set 2 primers for alTERC. Two bands of ~220 bp for mTERC and ~150bp for alTERC were observed. NTC- control, no cDNA. (A is a representative picture of 3 independent experiments). B. The PCR reaction described in A was carried out in the presence of radioactive nucleotide (dCTP [αp 32 ] and the reaction products were analysed by 14% polyacrylamide gel electrophoresis following autoradiography. Two bands of ~230 bp and ~210 bp were observed with set 1 primers (Fpr1) for mTERC and one band of ~150 bp with set primers 2 (Fpr2) for alTERC were detected. C. RNA was extracted from mouse NSC-34 motor neurons like cells followed by cDNA production in the presence or absence of DNase or RNase and subjected to PCR amplification as described in A using the set1 and set 2 primers. D. RNA was extracted from mouse organs (brain and spleen) or from mouse neuroblastoma cell line (N2a) and subjected to sqPCR analysis using the set 1 and 2 primers for TERC and alTERC and GAPDH primers as control. A Representative picture of 3 independent experiments. E. The results of experiments described in D were quantified by densitometric analysis with the EZQuant software, calculated relatively to GAPDH and the alTERC/TERC expression ratio was estimated. The data are means ± SD of 3 independent experiments.

    Article Snippet: In both cases DNA residues were removed using the “DNase I, RNase-free” kit (Thermo Fisher Scientific Inc, Pittsburgh PA, USA).

    Techniques: In Vivo, In Vitro, Generated, Polymerase Chain Reaction, Polyacrylamide Gel Electrophoresis, Autoradiography, Amplification, Software, Expressing

    RNase and DNase analysis of major satellite species. Total RNA from NIH/3T3 cells was treated with indicated enzymes for 30 min at 37°, phenol:chloroform extracted, ethanol precipitated and glyoxylated prior to electrophoresis, then blotted and probed for major satellite sequences, ethidium staining of ribosomal RNA is shown as a control. A: Samples treated in water without and with RNase A. B: Samples treated in NEBuffer 3 with indicated ribonucleases. C: Samples treated in RQ1 DNase buffer without and with DNase I. For quantification, error bars represent ±1 s.d., y-axes in arbitrary units, p values calculated using a one-way ANOVA (n = 4) for B and Student’s  t -test (n = 3) for C.

    Journal: PLoS ONE

    Article Title: Unexpected DNA Loss Mediated by the DNA Binding Activity of Ribonuclease A

    doi: 10.1371/journal.pone.0115008

    Figure Lengend Snippet: RNase and DNase analysis of major satellite species. Total RNA from NIH/3T3 cells was treated with indicated enzymes for 30 min at 37°, phenol:chloroform extracted, ethanol precipitated and glyoxylated prior to electrophoresis, then blotted and probed for major satellite sequences, ethidium staining of ribosomal RNA is shown as a control. A: Samples treated in water without and with RNase A. B: Samples treated in NEBuffer 3 with indicated ribonucleases. C: Samples treated in RQ1 DNase buffer without and with DNase I. For quantification, error bars represent ±1 s.d., y-axes in arbitrary units, p values calculated using a one-way ANOVA (n = 4) for B and Student’s t -test (n = 3) for C.

    Article Snippet: RNase A (Roche 10109142001) was dissolved at 10 mg/ml in 10 mM sodium acetate pH 5.2, boiled for 15 min then neutralised by addition of Tris pH 7.4 to 0.1 M then diluted to a 1 mg/ml stock, 10 mg/ml DNase-free RNase (Thermo EN0531) was used as provided (0.1 µl per reaction).

    Techniques: Electrophoresis, Staining

    Inhibition of DNA removal by RNase A. A: 0.5 ng major satellite PCR product was mixed with 1 µg NIH/3T3 RNA (lanes 1, 2) or with 1 µg DNA molecular weight marker (lanes 3, 4), and treated without or with RNase A followed by phenol:chloroform extraction and analysis as in Fig. 1. B: Identical to A, except that samples were incubated with 20 µg proteinase K for 15 min at 37° after RNase A treatment and phenol:chloroform extraction was omitted. For quantification, error bars represent ±1 s.d., y-axes in arbitrary units, analysis by one-way ANOVA (n = 3), differences in B are not significant.

    Journal: PLoS ONE

    Article Title: Unexpected DNA Loss Mediated by the DNA Binding Activity of Ribonuclease A

    doi: 10.1371/journal.pone.0115008

    Figure Lengend Snippet: Inhibition of DNA removal by RNase A. A: 0.5 ng major satellite PCR product was mixed with 1 µg NIH/3T3 RNA (lanes 1, 2) or with 1 µg DNA molecular weight marker (lanes 3, 4), and treated without or with RNase A followed by phenol:chloroform extraction and analysis as in Fig. 1. B: Identical to A, except that samples were incubated with 20 µg proteinase K for 15 min at 37° after RNase A treatment and phenol:chloroform extraction was omitted. For quantification, error bars represent ±1 s.d., y-axes in arbitrary units, analysis by one-way ANOVA (n = 3), differences in B are not significant.

    Article Snippet: RNase A (Roche 10109142001) was dissolved at 10 mg/ml in 10 mM sodium acetate pH 5.2, boiled for 15 min then neutralised by addition of Tris pH 7.4 to 0.1 M then diluted to a 1 mg/ml stock, 10 mg/ml DNase-free RNase (Thermo EN0531) was used as provided (0.1 µl per reaction).

    Techniques: Inhibition, Polymerase Chain Reaction, Molecular Weight, Marker, Incubation

    RNase A activity on DNA. A, B: 0.5 ng major satellite PCR product was mixed with 1 µg NIH/3T3 total RNA and analysed as in Fig. 1. A: Treatment with RNase A from different manufacturers. B: Treatment with RNase A in different buffers. C: 50 ng DNA molecular weight marker was mixed with 1 µg NIH/3T3 total RNA and treated without or with RNase A, purified as in Fig. 1 and separated on a non-denaturing 1xTBE gel before blotting and probing for the molecular weight marker. D: 32 P-labelled 50 bp ladder mixed with 1 µg RNA was treated without or with RNase A for 30 min at 37°, directly separated on an 8% PAGE gel and exposed to a phosphorimaging screen. For quantification, error bars represent ±1 s.d., y-axes in arbitrary units, p values were calculated by one-way ANOVA (A, B) or Student’s t -test (C, D), n = 3 in all cases.

    Journal: PLoS ONE

    Article Title: Unexpected DNA Loss Mediated by the DNA Binding Activity of Ribonuclease A

    doi: 10.1371/journal.pone.0115008

    Figure Lengend Snippet: RNase A activity on DNA. A, B: 0.5 ng major satellite PCR product was mixed with 1 µg NIH/3T3 total RNA and analysed as in Fig. 1. A: Treatment with RNase A from different manufacturers. B: Treatment with RNase A in different buffers. C: 50 ng DNA molecular weight marker was mixed with 1 µg NIH/3T3 total RNA and treated without or with RNase A, purified as in Fig. 1 and separated on a non-denaturing 1xTBE gel before blotting and probing for the molecular weight marker. D: 32 P-labelled 50 bp ladder mixed with 1 µg RNA was treated without or with RNase A for 30 min at 37°, directly separated on an 8% PAGE gel and exposed to a phosphorimaging screen. For quantification, error bars represent ±1 s.d., y-axes in arbitrary units, p values were calculated by one-way ANOVA (A, B) or Student’s t -test (C, D), n = 3 in all cases.

    Article Snippet: RNase A (Roche 10109142001) was dissolved at 10 mg/ml in 10 mM sodium acetate pH 5.2, boiled for 15 min then neutralised by addition of Tris pH 7.4 to 0.1 M then diluted to a 1 mg/ml stock, 10 mg/ml DNase-free RNase (Thermo EN0531) was used as provided (0.1 µl per reaction).

    Techniques: Activity Assay, Polymerase Chain Reaction, Molecular Weight, Marker, Purification, Polyacrylamide Gel Electrophoresis

    Re-partitioning of RNase-treated DNA. A, B: 5 ng 32 P-labelled major satellite PCR product mixed with 1 µg of NIH/3T3 RNA was incubated without or with RNase A for 30 min at 37°. Reactions were then diluted to 100 µl with water and extracted with 100 µl phenol:chloroform. A: 10 µl of the aqueous phase was analysed by electrophoresis in a non-denaturing 1xTBE gel which was dried and exposed to a phosphorimaging screen. B: Radioactivity present in the aqueous and phenol phases quantitated using a Geiger counter. C: DNA loss after RNase A treatment with phenol:chloroform extraction at pH 7 or pH 8. D: Activity of RNase A in phenol:chloroform. 1 µl 1 mg/ml RNase A was added to 100 µl phenol:chloroform and vortexed. 1 µl 1 mg/ml NIH/3T3 RNA was added, vortexed and reactions incubated at 37° for 30 min, followed by extraction with 100 µl water, precipitation and gel electrophoresis. For quantification, error bars represent ±1 s.d, y-axes in arbitrary units for A, D or Bequerels in B, analysis by Student’s t -test, n = 3.

    Journal: PLoS ONE

    Article Title: Unexpected DNA Loss Mediated by the DNA Binding Activity of Ribonuclease A

    doi: 10.1371/journal.pone.0115008

    Figure Lengend Snippet: Re-partitioning of RNase-treated DNA. A, B: 5 ng 32 P-labelled major satellite PCR product mixed with 1 µg of NIH/3T3 RNA was incubated without or with RNase A for 30 min at 37°. Reactions were then diluted to 100 µl with water and extracted with 100 µl phenol:chloroform. A: 10 µl of the aqueous phase was analysed by electrophoresis in a non-denaturing 1xTBE gel which was dried and exposed to a phosphorimaging screen. B: Radioactivity present in the aqueous and phenol phases quantitated using a Geiger counter. C: DNA loss after RNase A treatment with phenol:chloroform extraction at pH 7 or pH 8. D: Activity of RNase A in phenol:chloroform. 1 µl 1 mg/ml RNase A was added to 100 µl phenol:chloroform and vortexed. 1 µl 1 mg/ml NIH/3T3 RNA was added, vortexed and reactions incubated at 37° for 30 min, followed by extraction with 100 µl water, precipitation and gel electrophoresis. For quantification, error bars represent ±1 s.d, y-axes in arbitrary units for A, D or Bequerels in B, analysis by Student’s t -test, n = 3.

    Article Snippet: RNase A (Roche 10109142001) was dissolved at 10 mg/ml in 10 mM sodium acetate pH 5.2, boiled for 15 min then neutralised by addition of Tris pH 7.4 to 0.1 M then diluted to a 1 mg/ml stock, 10 mg/ml DNase-free RNase (Thermo EN0531) was used as provided (0.1 µl per reaction).

    Techniques: Polymerase Chain Reaction, Incubation, Electrophoresis, Radioactivity, Activity Assay, Nucleic Acid Electrophoresis

    p5RHH: Nox4 siRNA nanocomplex transfection downregulates Nox4 mRNA and protein expression in VSMC from 4-month old wild-type mice. (A) p5RHH: Nox4 siRNA nanocomplex transfection efficiency compared to RNAiMAX Nox4 siRNA using quantitative RT-PCR (mean ±

    Journal: Journal of molecular and cellular cardiology

    Article Title: NADPH oxidase 4 regulates vascular inflammation in aging and atherosclerosis

    doi: 10.1016/j.yjmcc.2016.12.004

    Figure Lengend Snippet: p5RHH: Nox4 siRNA nanocomplex transfection downregulates Nox4 mRNA and protein expression in VSMC from 4-month old wild-type mice. (A) p5RHH: Nox4 siRNA nanocomplex transfection efficiency compared to RNAiMAX Nox4 siRNA using quantitative RT-PCR (mean ±

    Article Snippet: Peptide-siRNA nanocomplexes were prepared by dilution of p5RHH (10 mM stock in DNAse-, RNAse-, and protease-free water (Sigma-Al-drich)) and siRNA (100 µM stock in siRNA buffer (Thermo Scientific)) 1:200 in Opti-MEM medium, followed by incubation for 40 min at 37 °C.

    Techniques: Transfection, Expressing, Mouse Assay, Quantitative RT-PCR

    p5RHH: Nox4 siRNA nanocomplex transfection downregulates H 2 O 2 production and shows low cytotoxicity in VSMCs from 4-month old wild-type mice. (A) H 2 O 2 production assayed with Amplex Red in media after 24 h incubation of VSMCs with TGFβ1 (mean ±

    Journal: Journal of molecular and cellular cardiology

    Article Title: NADPH oxidase 4 regulates vascular inflammation in aging and atherosclerosis

    doi: 10.1016/j.yjmcc.2016.12.004

    Figure Lengend Snippet: p5RHH: Nox4 siRNA nanocomplex transfection downregulates H 2 O 2 production and shows low cytotoxicity in VSMCs from 4-month old wild-type mice. (A) H 2 O 2 production assayed with Amplex Red in media after 24 h incubation of VSMCs with TGFβ1 (mean ±

    Article Snippet: Peptide-siRNA nanocomplexes were prepared by dilution of p5RHH (10 mM stock in DNAse-, RNAse-, and protease-free water (Sigma-Al-drich)) and siRNA (100 µM stock in siRNA buffer (Thermo Scientific)) 1:200 in Opti-MEM medium, followed by incubation for 40 min at 37 °C.

    Techniques: Transfection, Mouse Assay, Incubation

    p5RHH: Nox4 siRNA nanocomplex transfection of VSMCs attenuates TGFβ1-stimulated expression of pro-inflammatory genes from 4-month old wild-type mice. Relative mRNA expression was assessed by quantitative RT-PCR (mean ± SEM, n = 4); (A)

    Journal: Journal of molecular and cellular cardiology

    Article Title: NADPH oxidase 4 regulates vascular inflammation in aging and atherosclerosis

    doi: 10.1016/j.yjmcc.2016.12.004

    Figure Lengend Snippet: p5RHH: Nox4 siRNA nanocomplex transfection of VSMCs attenuates TGFβ1-stimulated expression of pro-inflammatory genes from 4-month old wild-type mice. Relative mRNA expression was assessed by quantitative RT-PCR (mean ± SEM, n = 4); (A)

    Article Snippet: Peptide-siRNA nanocomplexes were prepared by dilution of p5RHH (10 mM stock in DNAse-, RNAse-, and protease-free water (Sigma-Al-drich)) and siRNA (100 µM stock in siRNA buffer (Thermo Scientific)) 1:200 in Opti-MEM medium, followed by incubation for 40 min at 37 °C.

    Techniques: Transfection, Expressing, Mouse Assay, Quantitative RT-PCR

    TAK1, AP1, and canonical NFκB pathways regulate Nox4 expression in VSMCs from 4-month old mice. Relative Nox4 mRNA expression was assessed by quantitative RT-PCR (mean ± SEM, n = 4) in cells transfected with p5RHH:siRNA nanoparticles;

    Journal: Journal of molecular and cellular cardiology

    Article Title: NADPH oxidase 4 regulates vascular inflammation in aging and atherosclerosis

    doi: 10.1016/j.yjmcc.2016.12.004

    Figure Lengend Snippet: TAK1, AP1, and canonical NFκB pathways regulate Nox4 expression in VSMCs from 4-month old mice. Relative Nox4 mRNA expression was assessed by quantitative RT-PCR (mean ± SEM, n = 4) in cells transfected with p5RHH:siRNA nanoparticles;

    Article Snippet: Peptide-siRNA nanocomplexes were prepared by dilution of p5RHH (10 mM stock in DNAse-, RNAse-, and protease-free water (Sigma-Al-drich)) and siRNA (100 µM stock in siRNA buffer (Thermo Scientific)) 1:200 in Opti-MEM medium, followed by incubation for 40 min at 37 °C.

    Techniques: Expressing, Mouse Assay, Quantitative RT-PCR, Transfection