rnase free dnase set  (Qiagen)


Bioz Verified Symbol Qiagen is a verified supplier
Bioz Manufacturer Symbol Qiagen manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Name:
    RNase-Free DNase Set
    Description:

    Catalog Number:
    79254
    Price:
    None
    Score:
    85
    Buy from Supplier


    Structured Review

    Qiagen rnase free dnase set
    Extracellular vesicle internalization and transfer of GFP mRNA ( A ) Super resolution 3D reconstruction of GFP+ mRPC following 24 h incubation with PKH26 labeled extracellular vesicles. Red vesicles are visibly localized near the cell surface and within cytoplasm. In the XZ axis, GFP (green), EVs (red) and nuclei (blue, DAPI). ( B ) same as ( A ) with GFP (FITC) channel removed to increase visibility of PKH26 (TRITC) labeled EVs. Each panel contains three cross-sectional views (xy, xz, and yz). Scale: 5 µm. ( C ) RT-PCR analysis of GFP mRNA transfer between GFP+ mRPCs and non-GFP hRPCs. Non-GFP hRPCs served as negative control; GFP+ mRPCs served as postive control. GAPDH served as the internal control gene. EVs were treated using an <t>RNase-Free</t> <t>DNase</t> Set to remove DNA comtamination before cDNA synthesis. ( D ) Intensities of RT-PCR images were measured with ImageJ software and normalized to GAPDH. Relative levels of hRPC GFP after transfer of EVs is significantly higher than negative control.

    https://www.bioz.com/result/rnase free dnase set/product/Qiagen
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rnase free dnase set - by Bioz Stars, 2019-10
    99/100 stars

    Related Products / Commonly Used Together

    rneasy mini

    Images

    1) Product Images from "Retinal progenitor cells release extracellular vesicles containing developmental transcription factors, microRNA and membrane proteins"

    Article Title: Retinal progenitor cells release extracellular vesicles containing developmental transcription factors, microRNA and membrane proteins

    Journal: Scientific Reports

    doi: 10.1038/s41598-018-20421-1

    Extracellular vesicle internalization and transfer of GFP mRNA ( A ) Super resolution 3D reconstruction of GFP+ mRPC following 24 h incubation with PKH26 labeled extracellular vesicles. Red vesicles are visibly localized near the cell surface and within cytoplasm. In the XZ axis, GFP (green), EVs (red) and nuclei (blue, DAPI). ( B ) same as ( A ) with GFP (FITC) channel removed to increase visibility of PKH26 (TRITC) labeled EVs. Each panel contains three cross-sectional views (xy, xz, and yz). Scale: 5 µm. ( C ) RT-PCR analysis of GFP mRNA transfer between GFP+ mRPCs and non-GFP hRPCs. Non-GFP hRPCs served as negative control; GFP+ mRPCs served as postive control. GAPDH served as the internal control gene. EVs were treated using an RNase-Free DNase Set to remove DNA comtamination before cDNA synthesis. ( D ) Intensities of RT-PCR images were measured with ImageJ software and normalized to GAPDH. Relative levels of hRPC GFP after transfer of EVs is significantly higher than negative control.
    Figure Legend Snippet: Extracellular vesicle internalization and transfer of GFP mRNA ( A ) Super resolution 3D reconstruction of GFP+ mRPC following 24 h incubation with PKH26 labeled extracellular vesicles. Red vesicles are visibly localized near the cell surface and within cytoplasm. In the XZ axis, GFP (green), EVs (red) and nuclei (blue, DAPI). ( B ) same as ( A ) with GFP (FITC) channel removed to increase visibility of PKH26 (TRITC) labeled EVs. Each panel contains three cross-sectional views (xy, xz, and yz). Scale: 5 µm. ( C ) RT-PCR analysis of GFP mRNA transfer between GFP+ mRPCs and non-GFP hRPCs. Non-GFP hRPCs served as negative control; GFP+ mRPCs served as postive control. GAPDH served as the internal control gene. EVs were treated using an RNase-Free DNase Set to remove DNA comtamination before cDNA synthesis. ( D ) Intensities of RT-PCR images were measured with ImageJ software and normalized to GAPDH. Relative levels of hRPC GFP after transfer of EVs is significantly higher than negative control.

    Techniques Used: Incubation, Labeling, Reverse Transcription Polymerase Chain Reaction, Negative Control, Software

    2) Product Images from "MicroRNAs Circulate in the Hemolymph of Drosophila and Accumulate Relative to Tissue microRNAs in an Age-Dependent Manner"

    Article Title: MicroRNAs Circulate in the Hemolymph of Drosophila and Accumulate Relative to Tissue microRNAs in an Age-Dependent Manner

    Journal: Genomics Insights

    doi: 10.4137/GEI.S38147

    Presence of stable miRNAs in Drosophila melanogaster hemolymph. ( A , B ) Clear HL droplets extruded from fly head and thorax. ( C – F ) Real-time qPCR amplification of selected HL miRNAs and mRNAs. Total RNA including small RNA was extracted from HL samples and analyzed with qPCR to measure the levels of miRNAs and mRNAs. The y -axis represents the relative fluorescence units (RFU) in a semi-log scale. The x -axis represents the cycle at which fluorescence was detected above an automatically determined threshold. ( C ) Amplification plots for miR-14, miR-8, and miR-184 measured in a representative HL sample. ( D ) Amplification plots for miR-14, let-7, bantam, and spiked-in synthetic C. elegans cel-miR-39 RNA in representative HL sample. ( E ) The amplification plots for tubulin, actin, and gapdh mRNAs determined by qPCR using total RNA from HL and S2 cells. Sample from S2 cells are used as a positive control for detecting Drosophila mRNAs by qPCR. The amplification curves of all three mRNAs are superimposed on one another, reflecting the presence of similar amounts of these mRNA in S2 cells. Amplification curves from HL samples show that fluorescent products appear after about 30 cycles, reflecting the significantly lower abundance of these mRNAs in HL relative to S2 cells. ( F ) The cycle threshold (Ct) fold-change of selected miRNA amplified in the absence or presence of RNase A and DNase I. Total RNA was extracted from HL samples spiked with 10 fmoles of cel-miR-39 RNA. The x -axis represents the ratio of raw Ct values from control samples divided by raw Ct values from samples incubated with RNase A and DNase I. The significantly higher magnitude of the Ct fold change of the spiked-in synthetic miRNA relative to those of the miRNA indicates that the HL-miRNA are present in nuclease-resistant, stable form.
    Figure Legend Snippet: Presence of stable miRNAs in Drosophila melanogaster hemolymph. ( A , B ) Clear HL droplets extruded from fly head and thorax. ( C – F ) Real-time qPCR amplification of selected HL miRNAs and mRNAs. Total RNA including small RNA was extracted from HL samples and analyzed with qPCR to measure the levels of miRNAs and mRNAs. The y -axis represents the relative fluorescence units (RFU) in a semi-log scale. The x -axis represents the cycle at which fluorescence was detected above an automatically determined threshold. ( C ) Amplification plots for miR-14, miR-8, and miR-184 measured in a representative HL sample. ( D ) Amplification plots for miR-14, let-7, bantam, and spiked-in synthetic C. elegans cel-miR-39 RNA in representative HL sample. ( E ) The amplification plots for tubulin, actin, and gapdh mRNAs determined by qPCR using total RNA from HL and S2 cells. Sample from S2 cells are used as a positive control for detecting Drosophila mRNAs by qPCR. The amplification curves of all three mRNAs are superimposed on one another, reflecting the presence of similar amounts of these mRNA in S2 cells. Amplification curves from HL samples show that fluorescent products appear after about 30 cycles, reflecting the significantly lower abundance of these mRNAs in HL relative to S2 cells. ( F ) The cycle threshold (Ct) fold-change of selected miRNA amplified in the absence or presence of RNase A and DNase I. Total RNA was extracted from HL samples spiked with 10 fmoles of cel-miR-39 RNA. The x -axis represents the ratio of raw Ct values from control samples divided by raw Ct values from samples incubated with RNase A and DNase I. The significantly higher magnitude of the Ct fold change of the spiked-in synthetic miRNA relative to those of the miRNA indicates that the HL-miRNA are present in nuclease-resistant, stable form.

    Techniques Used: Real-time Polymerase Chain Reaction, Amplification, Fluorescence, Positive Control, Incubation

    3) Product Images from "Transparent DNA/RNA Co-extraction Workflow Protocol Suitable for Inhibitor-Rich Environmental Samples That Focuses on Complete DNA Removal for Transcriptomic Analyses"

    Article Title: Transparent DNA/RNA Co-extraction Workflow Protocol Suitable for Inhibitor-Rich Environmental Samples That Focuses on Complete DNA Removal for Transcriptomic Analyses

    Journal: Frontiers in Microbiology

    doi: 10.3389/fmicb.2016.01588

    Suggested DNA/RNA co-extraction workflow for environmental samples, with stronger emphasis on thorough purification prior to all enzymatic steps (including DNase digestion). Optional steps are indicated by dotted arrows. Note that RNase digestion (between Extracts II and III) may be necessary for better results downstream, but may be omitted as a separate step (in the current study, RNase is present in the qPCR mix). (A) Pre-lysis inhibitor removal is only advisable if quick methods are used, or if mRNA is not the target molecule (lengthy inhibitor removal procedures compromise RNA integrity). (B) Various methods may be used, such as phenol/chloroform procedures or nucleic acid precipitation. (C) This purification step should target the removal of enzymatic-inhibitors (e.g., humic/fulvic acids and polyphenolics). (D) Purification of partially digested RNA extracts with residual genomic DNA aids in the removal of enduring inhibitors, prior to further digestion. (E) Stringent and well-documented quality control via rigorous and sensitive detection (preferably quantitative methods) is necessary to detect residual amplifiable gDNA prior to reverse transcription.
    Figure Legend Snippet: Suggested DNA/RNA co-extraction workflow for environmental samples, with stronger emphasis on thorough purification prior to all enzymatic steps (including DNase digestion). Optional steps are indicated by dotted arrows. Note that RNase digestion (between Extracts II and III) may be necessary for better results downstream, but may be omitted as a separate step (in the current study, RNase is present in the qPCR mix). (A) Pre-lysis inhibitor removal is only advisable if quick methods are used, or if mRNA is not the target molecule (lengthy inhibitor removal procedures compromise RNA integrity). (B) Various methods may be used, such as phenol/chloroform procedures or nucleic acid precipitation. (C) This purification step should target the removal of enzymatic-inhibitors (e.g., humic/fulvic acids and polyphenolics). (D) Purification of partially digested RNA extracts with residual genomic DNA aids in the removal of enduring inhibitors, prior to further digestion. (E) Stringent and well-documented quality control via rigorous and sensitive detection (preferably quantitative methods) is necessary to detect residual amplifiable gDNA prior to reverse transcription.

    Techniques Used: Environmental Sampling, Purification, Real-time Polymerase Chain Reaction, Lysis

    Related Articles

    Clone Assay:

    Article Title: Overexpression of StDREB2 Transcription Factor Enhances Drought Stress Tolerance in Cotton (Gossypium barbadense L.)
    Article Snippet: RNase-Free DNase Set (Qiagen) was then used to remove the contaminating DNA. .. RNase-Free DNase Set (Qiagen) was then used to remove the contaminating DNA.

    Centrifugation:

    Article Title: Campylobacter coli From Retail Liver and Meat Products Is More Aerotolerant Than Campylobacter jejuni
    Article Snippet: For total RNA isolation, 2 ml RNA protect bacteria reagent (Qiagen) was added to 1 ml of bacterial culture and vortexed for 5 s followed by incubation at room temperature for 5 min. Bacterial cell was harvested by centrifugation at 5,000 × g for 5 min at 4°C and cell pellet was resuspended in 700 μl of lysis buffer (RLT buffer, Qiagen) by vortexing for 10 s. Bacterial cell lysis was done by vortexing vigorously for 5 min in 2 ml safe lock tube containing ~30 mg acid washed glass beads (212–300 μm, Sigma, G1277). .. After washing and elution steps according to manufacturer's protocol, RNA samples were further subjected to DNA digestion in solution using RNase Free DNase set (Qiagen).

    Amplification:

    Article Title: Laser capture microdissection in Ectocarpus siliculosus: the pathway to cell-specific transcriptomics in brown algae
    Article Snippet: On column DNA digestion was carried out using Qiagen RNase-free DNase I set (#79254). .. On column DNA digestion was carried out using Qiagen RNase-free DNase I set (#79254).

    Article Title: Phenotype and Susceptibility to HIV-infection of CD4+ Th17 Cells in the Human Female Reproductive Tract
    Article Snippet: DNase digestion was done on-column with the RNase-Free DNase set (Qiagen). .. DNase digestion was done on-column with the RNase-Free DNase set (Qiagen).

    Article Title: A role for the Tgf- β/Bmp co-receptor Endoglin in the molecular oscillator that regulates the hair follicle cycle
    Article Snippet: For RNA extraction, E14 cell cultures coming from the treatments described above, or whole dorsal skin of at least three mice at each time point and genotype, RNeasy mini kit and RNase-Free DNase Set (both from Qiagen) were used. .. For RNA extraction, E14 cell cultures coming from the treatments described above, or whole dorsal skin of at least three mice at each time point and genotype, RNeasy mini kit and RNase-Free DNase Set (both from Qiagen) were used.

    Article Title: Overexpression of StDREB2 Transcription Factor Enhances Drought Stress Tolerance in Cotton (Gossypium barbadense L.)
    Article Snippet: RNase-Free DNase Set (Qiagen) was then used to remove the contaminating DNA. .. RNase-Free DNase Set (Qiagen) was then used to remove the contaminating DNA.

    Article Title: Hyperplasia of Interstitial Cells of Cajal in Sprouty Homolog 4 Deficient Mice
    Article Snippet: Genomic DNA was removed using the RNase-Free DNase set (Qiagen). .. Genomic DNA was removed using the RNase-Free DNase set (Qiagen).

    Synthesized:

    Article Title: Stem Cells Derived from Lipoma and Adipose Tissue—Similar Mesenchymal Phenotype but Different Differentiation Capacity Governed by Distinct Molecular Signature
    Article Snippet: DNase I RNase-free set (Qiagen) was used for on-column digestion of residual genomic DNA, according to the manufacturer’s instructions. .. Reverse transcription was performed in the PCR thermal cycler SureCycler8800 (Agilent Technologies, Santa Clara, CA, USA).

    Quantitative RT-PCR:

    Article Title: Down-regulation of AR splice variants through XPO1 suppression contributes to the inhibition of prostate cancer progression
    Article Snippet: Paragraph title: RNA isolation and mRNA real-time RT-qPCR ... Total RNAs from PCa cell lines treated with SINE were extracted and purified by using the RNeasy Mini Kit and RNase-free DNase Set (QIAGEN, Valencia, CA) following the protocol provided by the manufacturer.

    Article Title: A role for the Tgf- β/Bmp co-receptor Endoglin in the molecular oscillator that regulates the hair follicle cycle
    Article Snippet: For RNA extraction, E14 cell cultures coming from the treatments described above, or whole dorsal skin of at least three mice at each time point and genotype, RNeasy mini kit and RNase-Free DNase Set (both from Qiagen) were used. .. Skin tissue was homogenized using TriPureTM isolation Reagent (Roche), disaggregated and processed using scissors and a Polytron® homogenizer (PT 1200 E, Kinematica).

    Article Title: Effects of Obesity and Metabolic Syndrome on Steroidogenesis and Folliculogenesis in the Female Ossabaw Mini-Pig
    Article Snippet: Total RNA was extracted from cell aliquots of 100,000 cells using the RNeasy Mini Kit (Qiagen Inc) and an on-column DNase treatment (RNase-free DNase Set, Qiagen Inc). .. Total RNA was extracted from cell aliquots of 100,000 cells using the RNeasy Mini Kit (Qiagen Inc) and an on-column DNase treatment (RNase-free DNase Set, Qiagen Inc).

    SYBR Green Assay:

    Article Title: Down-regulation of AR splice variants through XPO1 suppression contributes to the inhibition of prostate cancer progression
    Article Snippet: Total RNAs from PCa cell lines treated with SINE were extracted and purified by using the RNeasy Mini Kit and RNase-free DNase Set (QIAGEN, Valencia, CA) following the protocol provided by the manufacturer. .. Total RNAs from PCa cell lines treated with SINE were extracted and purified by using the RNeasy Mini Kit and RNase-free DNase Set (QIAGEN, Valencia, CA) following the protocol provided by the manufacturer.

    Article Title: A role for the Tgf- β/Bmp co-receptor Endoglin in the molecular oscillator that regulates the hair follicle cycle
    Article Snippet: For RNA extraction, E14 cell cultures coming from the treatments described above, or whole dorsal skin of at least three mice at each time point and genotype, RNeasy mini kit and RNase-Free DNase Set (both from Qiagen) were used. .. Skin tissue was homogenized using TriPureTM isolation Reagent (Roche), disaggregated and processed using scissors and a Polytron® homogenizer (PT 1200 E, Kinematica).

    Article Title: Hyperplasia of Interstitial Cells of Cajal in Sprouty Homolog 4 Deficient Mice
    Article Snippet: Genomic DNA was removed using the RNase-Free DNase set (Qiagen). .. Genomic DNA was removed using the RNase-Free DNase set (Qiagen).

    Microarray:

    Article Title: A role for the Tgf- β/Bmp co-receptor Endoglin in the molecular oscillator that regulates the hair follicle cycle
    Article Snippet: For RNA extraction, E14 cell cultures coming from the treatments described above, or whole dorsal skin of at least three mice at each time point and genotype, RNeasy mini kit and RNase-Free DNase Set (both from Qiagen) were used. .. Specific primers, detailed in , were designed among different exons, thereby avoiding residual genomic DNA amplification, for semiquantitative or quantitative transcript detection.

    Incubation:

    Article Title: Campylobacter coli From Retail Liver and Meat Products Is More Aerotolerant Than Campylobacter jejuni
    Article Snippet: For total RNA isolation, 2 ml RNA protect bacteria reagent (Qiagen) was added to 1 ml of bacterial culture and vortexed for 5 s followed by incubation at room temperature for 5 min. Bacterial cell was harvested by centrifugation at 5,000 × g for 5 min at 4°C and cell pellet was resuspended in 700 μl of lysis buffer (RLT buffer, Qiagen) by vortexing for 10 s. Bacterial cell lysis was done by vortexing vigorously for 5 min in 2 ml safe lock tube containing ~30 mg acid washed glass beads (212–300 μm, Sigma, G1277). .. After washing and elution steps according to manufacturer's protocol, RNA samples were further subjected to DNA digestion in solution using RNase Free DNase set (Qiagen).

    Article Title: Time-resolved mapping of genetic interactions to model rewiring of signaling pathways
    Article Snippet: After 72 hr incubation (25°C, no CO2 adjustment), cells were washed once in 750 µl PBS (Gibco) and lysed in 350 µl RLT buffer shipped with the RNAeasy-mini Kit (Qiagen). .. An optional DNase digestion step was performed using the RNase-Free DNase Set (Qiagen).

    Expressing:

    Article Title: Repurposing Ponatinib as a Potent Agent against KIT Mutant Melanomas
    Article Snippet: Qualified total RNA was further purified by RNAClean XP Kit (Cat A63987, Beckman Coulter,Inc.Kraemer Boulevard Brea, CA,USA)and RNase-Free DNase Set (Cat#79254, QIAGEN, GmBH, Germany). .. Qualified total RNA was further purified by RNAClean XP Kit (Cat A63987, Beckman Coulter,Inc.Kraemer Boulevard Brea, CA,USA)and RNase-Free DNase Set (Cat#79254, QIAGEN, GmBH, Germany).

    Article Title: Stem Cells Derived from Lipoma and Adipose Tissue—Similar Mesenchymal Phenotype but Different Differentiation Capacity Governed by Distinct Molecular Signature
    Article Snippet: DNase I RNase-free set (Qiagen) was used for on-column digestion of residual genomic DNA, according to the manufacturer’s instructions. .. Reverse transcription was performed in the PCR thermal cycler SureCycler8800 (Agilent Technologies, Santa Clara, CA, USA).

    Article Title: Down-regulation of AR splice variants through XPO1 suppression contributes to the inhibition of prostate cancer progression
    Article Snippet: Total RNAs from PCa cell lines treated with SINE were extracted and purified by using the RNeasy Mini Kit and RNase-free DNase Set (QIAGEN, Valencia, CA) following the protocol provided by the manufacturer. .. Total RNAs from PCa cell lines treated with SINE were extracted and purified by using the RNeasy Mini Kit and RNase-free DNase Set (QIAGEN, Valencia, CA) following the protocol provided by the manufacturer.

    Article Title: A role for the Tgf- β/Bmp co-receptor Endoglin in the molecular oscillator that regulates the hair follicle cycle
    Article Snippet: Paragraph title: Gene expression procedures ... For RNA extraction, E14 cell cultures coming from the treatments described above, or whole dorsal skin of at least three mice at each time point and genotype, RNeasy mini kit and RNase-Free DNase Set (both from Qiagen) were used.

    Article Title: Analysis of ADP-glucose pyrophosphorylase expression during turion formation induced by abscisic acid in Spirodela polyrhiza (greater duckweed)
    Article Snippet: Considering that only daughter fronds shorter than 0.7 mm in length respond to ABA treatment and undergo turion formation after ABA treatment [ ], developing turions only with specific sizes were collected at their developmental stages after 0 (no ABA addition), 1, 2, 3, 5, 7 days of ABA treatment, respectively, for quantification of APL gene expression (Table ). .. The on-column DNase I was used to remove contaminating genomic DNA (Qiagen, 79254).

    Article Title: Effects of Obesity and Metabolic Syndrome on Steroidogenesis and Folliculogenesis in the Female Ossabaw Mini-Pig
    Article Snippet: Total RNA was extracted from cell aliquots of 100,000 cells using the RNeasy Mini Kit (Qiagen Inc) and an on-column DNase treatment (RNase-free DNase Set, Qiagen Inc). .. Total RNA was extracted from cell aliquots of 100,000 cells using the RNeasy Mini Kit (Qiagen Inc) and an on-column DNase treatment (RNase-free DNase Set, Qiagen Inc).

    RNA Sequencing Assay:

    Article Title: Repurposing Ponatinib as a Potent Agent against KIT Mutant Melanomas
    Article Snippet: Paragraph title: RNA-Sequencing and analysis ... Qualified total RNA was further purified by RNAClean XP Kit (Cat A63987, Beckman Coulter,Inc.Kraemer Boulevard Brea, CA,USA)and RNase-Free DNase Set (Cat#79254, QIAGEN, GmBH, Germany).

    Article Title: Laser capture microdissection in Ectocarpus siliculosus: the pathway to cell-specific transcriptomics in brown algae
    Article Snippet: On column DNA digestion was carried out using Qiagen RNase-free DNase I set (#79254). .. On column DNA digestion was carried out using Qiagen RNase-free DNase I set (#79254).

    Article Title: The transcriptome sequencing and functional analysis of eyestalk ganglions in Chinese mitten crab (Eriocheir sinensis) treated with different photoperiods
    Article Snippet: Paragraph title: RNA extracted, read alignment and RNA-seq analysis ... Qualified total RNA was further purified with a RNeasy micro kit (Cat#74004, QIAGEN, GmBH, Germany) and RNase-Free DNase Set (Cat#79254, QIAGEN, GmBH, Germany).

    Transformation Assay:

    Article Title: Overexpression of StDREB2 Transcription Factor Enhances Drought Stress Tolerance in Cotton (Gossypium barbadense L.)
    Article Snippet: Paragraph title: 2.2. Vector Construction and Cotton Transformation ... RNase-Free DNase Set (Qiagen) was then used to remove the contaminating DNA.

    Flow Cytometry:

    Article Title: Campylobacter coli From Retail Liver and Meat Products Is More Aerotolerant Than Campylobacter jejuni
    Article Snippet: Then, 700 μl of lysate was transferred to RNeasy spin column and centrifuged for 15 s at 13,800 × g. Flow through was discarded and remaining lysate solution was added and centrifuged in the same column. .. After washing and elution steps according to manufacturer's protocol, RNA samples were further subjected to DNA digestion in solution using RNase Free DNase set (Qiagen).

    Infection:

    Article Title: Intrinsic terminators in Mycoplasma hyopneumoniae transcription
    Article Snippet: M. hyopneumoniae strain 7448 isolated from an infected swine (Embrapa, Santa Catarina, Brazil) [ ] was grown in 25 ml of Friis broth [ ] at 37°C for 24 h with gentle agitation in a roller drum. .. The purification was performed according to the manufacturer’s instructions, with on-column DNaseI digestion using the RNase-Free DNase Set (Qiagen, Germany) and a second round of treatment with DNase I (Fermentas, USA).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Analysis of ADP-glucose pyrophosphorylase expression during turion formation induced by abscisic acid in Spirodela polyrhiza (greater duckweed)
    Article Snippet: The on-column DNase I was used to remove contaminating genomic DNA (Qiagen, 79254). .. The on-column DNase I was used to remove contaminating genomic DNA (Qiagen, 79254).

    Generated:

    Article Title: Analysis of ADP-glucose pyrophosphorylase expression during turion formation induced by abscisic acid in Spirodela polyrhiza (greater duckweed)
    Article Snippet: The on-column DNase I was used to remove contaminating genomic DNA (Qiagen, 79254). .. The on-column DNase I was used to remove contaminating genomic DNA (Qiagen, 79254).

    Article Title: Hyperplasia of Interstitial Cells of Cajal in Sprouty Homolog 4 Deficient Mice
    Article Snippet: Genomic DNA was removed using the RNase-Free DNase set (Qiagen). .. Genomic DNA was removed using the RNase-Free DNase set (Qiagen).

    Polymerase Chain Reaction:

    Article Title: Campylobacter coli From Retail Liver and Meat Products Is More Aerotolerant Than Campylobacter jejuni
    Article Snippet: After washing and elution steps according to manufacturer's protocol, RNA samples were further subjected to DNA digestion in solution using RNase Free DNase set (Qiagen). .. Quantity of total RNA was measured with NanoDrop™ 8,000 spectrophotometer.

    Article Title: Stem Cells Derived from Lipoma and Adipose Tissue—Similar Mesenchymal Phenotype but Different Differentiation Capacity Governed by Distinct Molecular Signature
    Article Snippet: DNase I RNase-free set (Qiagen) was used for on-column digestion of residual genomic DNA, according to the manufacturer’s instructions. .. DNase I RNase-free set (Qiagen) was used for on-column digestion of residual genomic DNA, according to the manufacturer’s instructions.

    Article Title: Laser capture microdissection in Ectocarpus siliculosus: the pathway to cell-specific transcriptomics in brown algae
    Article Snippet: On column DNA digestion was carried out using Qiagen RNase-free DNase I set (#79254). .. On column DNA digestion was carried out using Qiagen RNase-free DNase I set (#79254).

    Article Title: Time-resolved mapping of genetic interactions to model rewiring of signaling pathways
    Article Snippet: An optional DNase digestion step was performed using the RNase-Free DNase Set (Qiagen). .. A qPCR reaction was prepared using PrimaQuant 2x qPCR-Mastermix (Steinbrenner) by mixing 5 µl of sample (1:10 diluted cDNA) with 5 µl of Mastermix (including 0,3 µM of forward and reverse primer, ) on a 384-well qPCR plate (LightCycler 480 Multiwell Plate 384, white, Roche).

    Article Title: A role for the Tgf- β/Bmp co-receptor Endoglin in the molecular oscillator that regulates the hair follicle cycle
    Article Snippet: For RNA extraction, E14 cell cultures coming from the treatments described above, or whole dorsal skin of at least three mice at each time point and genotype, RNeasy mini kit and RNase-Free DNase Set (both from Qiagen) were used. .. Skin tissue was homogenized using TriPureTM isolation Reagent (Roche), disaggregated and processed using scissors and a Polytron® homogenizer (PT 1200 E, Kinematica).

    Nucleic Acid Electrophoresis:

    Article Title: Intrinsic terminators in Mycoplasma hyopneumoniae transcription
    Article Snippet: The purification was performed according to the manufacturer’s instructions, with on-column DNaseI digestion using the RNase-Free DNase Set (Qiagen, Germany) and a second round of treatment with DNase I (Fermentas, USA). .. DNA absence was monitored to below PCR-detectable levels.

    Gene Knockout:

    Article Title: Hyperplasia of Interstitial Cells of Cajal in Sprouty Homolog 4 Deficient Mice
    Article Snippet: A minimum of three different RNA samples from P10 WT, Kit K641E/K641E and Spry4 KO mice antrum were used. .. Genomic DNA was removed using the RNase-Free DNase set (Qiagen).

    Fluorescence:

    Article Title: Time-resolved mapping of genetic interactions to model rewiring of signaling pathways
    Article Snippet: An optional DNase digestion step was performed using the RNase-Free DNase Set (Qiagen). .. A qPCR reaction was prepared using PrimaQuant 2x qPCR-Mastermix (Steinbrenner) by mixing 5 µl of sample (1:10 diluted cDNA) with 5 µl of Mastermix (including 0,3 µM of forward and reverse primer, ) on a 384-well qPCR plate (LightCycler 480 Multiwell Plate 384, white, Roche).

    Article Title: Hyperplasia of Interstitial Cells of Cajal in Sprouty Homolog 4 Deficient Mice
    Article Snippet: Genomic DNA was removed using the RNase-Free DNase set (Qiagen). .. Genomic DNA was removed using the RNase-Free DNase set (Qiagen).

    Magnetic Beads:

    Article Title: The transcriptome sequencing and functional analysis of eyestalk ganglions in Chinese mitten crab (Eriocheir sinensis) treated with different photoperiods
    Article Snippet: Qualified total RNA was further purified with a RNeasy micro kit (Cat#74004, QIAGEN, GmBH, Germany) and RNase-Free DNase Set (Cat#79254, QIAGEN, GmBH, Germany). .. The RNA integrity numbers (RINs) of samples was required to be more than 7.0 when RNA-seq transcriptome libraries were prepared.

    Isolation:

    Article Title: Repurposing Ponatinib as a Potent Agent against KIT Mutant Melanomas
    Article Snippet: RNA was isolated from snap-frozen tumors from vehicle-, imatinib-, and ponatinib-treated mice. .. Qualified total RNA was further purified by RNAClean XP Kit (Cat A63987, Beckman Coulter,Inc.Kraemer Boulevard Brea, CA,USA)and RNase-Free DNase Set (Cat#79254, QIAGEN, GmBH, Germany).

    Article Title: Bioinformatics Analyses Determined the Distinct CNS and Peripheral Surrogate Biomarker Candidates Between Two Mouse Models for Progressive Multiple Sclerosis
    Article Snippet: Total RNA was extracted with an RNeasy Mini Kit (Qiagen, Germantown, MD) according to the manufacturer's instructions from brain and spleen homogenate. .. DNase treatment was performed during RNA isolation with an RNase-Free DNase Set (Qiagen). .. All samples were purified to an absorbance ratio (A260/A280) between 1.9 and 2.1 ( ).

    Article Title: Campylobacter coli From Retail Liver and Meat Products Is More Aerotolerant Than Campylobacter jejuni
    Article Snippet: Paragraph title: Isolation of Total RNA ... After washing and elution steps according to manufacturer's protocol, RNA samples were further subjected to DNA digestion in solution using RNase Free DNase set (Qiagen).

    Article Title: Stem Cells Derived from Lipoma and Adipose Tissue—Similar Mesenchymal Phenotype but Different Differentiation Capacity Governed by Distinct Molecular Signature
    Article Snippet: Paragraph title: 2.6. RNA Isolation and Reverse Transcription ... DNase I RNase-free set (Qiagen) was used for on-column digestion of residual genomic DNA, according to the manufacturer’s instructions.

    Article Title: Myc-like transcriptional factors in wheat: structural and functional organization of the subfamily I members
    Article Snippet: RNAs from coleoptile samples were extracted using the RNeasy Mini Kit (QIAGEN). .. All isolated RNAs were treated with the RNase-free DNase set (QIAGEN). .. Total RNA was converted to single-stranded cDNA in a 20-μL reaction from a template consisting of 0.5 μg of total RNA using the RevertAid First Strand cDNA Synthesis Kit (Thermo Fisher Scientific Inc.).

    Article Title: Down-regulation of AR splice variants through XPO1 suppression contributes to the inhibition of prostate cancer progression
    Article Snippet: Paragraph title: RNA isolation and mRNA real-time RT-qPCR ... Total RNAs from PCa cell lines treated with SINE were extracted and purified by using the RNeasy Mini Kit and RNase-free DNase Set (QIAGEN, Valencia, CA) following the protocol provided by the manufacturer.

    Article Title: Phenotype and Susceptibility to HIV-infection of CD4+ Th17 Cells in the Human Female Reproductive Tract
    Article Snippet: Paragraph title: RNA isolation and real time PCR ... DNase digestion was done on-column with the RNase-Free DNase set (Qiagen).

    Article Title: Definition of the ?W Regulon of Bacillus subtilis in the Absence of Stress
    Article Snippet: Precipitated RNA was washed with 70% ethanol and dissolved in 100 µl of RNase free water. .. The isolated RNA was DNase-treated using the RNase-Free DNase Set (Qiagen) and purified using the RNA Clean-Up and Concentration Micro Kit (Norgen). .. RNA concentrations were measured using a Nanodrop-1000 spectrophotometer and RNA quality was assessed with the Agilent 2100 Bioanalyzer according to the manufacturer's instructions.

    Article Title: Analysis of ADP-glucose pyrophosphorylase expression during turion formation induced by abscisic acid in Spirodela polyrhiza (greater duckweed)
    Article Snippet: Paragraph title: Genomic DNA and total RNA isolation ... The on-column DNase I was used to remove contaminating genomic DNA (Qiagen, 79254).

    Article Title: The Paracrine Feedback Loop Between Vitamin D3 (1,25(OH)2D3) and PTHrP in Prehypertrophic Chondrocytes
    Article Snippet: Paragraph title: RNA isolation and cDNA synthesis ... An additional DNA digestion step with DNase (RNAse-Free DNase Set, 79254, Qiagen) was included to ensure DNA removal.

    Article Title: Intrinsic terminators in Mycoplasma hyopneumoniae transcription
    Article Snippet: Paragraph title: Culture conditions and RNA isolation ... The purification was performed according to the manufacturer’s instructions, with on-column DNaseI digestion using the RNase-Free DNase Set (Qiagen, Germany) and a second round of treatment with DNase I (Fermentas, USA).

    Transfection:

    Article Title: Time-resolved mapping of genetic interactions to model rewiring of signaling pathways
    Article Snippet: To this end, as 5*105 cells / well were seeded in 630 µl ExpressFive (Gibco) culture medium and reverse transfected with 14 µg dsRNA. .. An optional DNase digestion step was performed using the RNase-Free DNase Set (Qiagen).

    Labeling:

    Article Title: Definition of the ?W Regulon of Bacillus subtilis in the Absence of Stress
    Article Snippet: The isolated RNA was DNase-treated using the RNase-Free DNase Set (Qiagen) and purified using the RNA Clean-Up and Concentration Micro Kit (Norgen). .. RNA concentrations were measured using a Nanodrop-1000 spectrophotometer and RNA quality was assessed with the Agilent 2100 Bioanalyzer according to the manufacturer's instructions.

    Mouse Assay:

    Article Title: Repurposing Ponatinib as a Potent Agent against KIT Mutant Melanomas
    Article Snippet: RNA was isolated from snap-frozen tumors from vehicle-, imatinib-, and ponatinib-treated mice. .. Qualified total RNA was further purified by RNAClean XP Kit (Cat A63987, Beckman Coulter,Inc.Kraemer Boulevard Brea, CA,USA)and RNase-Free DNase Set (Cat#79254, QIAGEN, GmBH, Germany).

    Article Title: Bioinformatics Analyses Determined the Distinct CNS and Peripheral Surrogate Biomarker Candidates Between Two Mouse Models for Progressive Multiple Sclerosis
    Article Snippet: Brains and spleens from three to six mice per group were homogenized individually in TRI-Reagent® (Molecular Research Center, Cincinnati, OH), using the Kinematica Polytron™ homogenizer (Kinematica, Bohemia, NY). .. DNase treatment was performed during RNA isolation with an RNase-Free DNase Set (Qiagen).

    Article Title: A role for the Tgf- β/Bmp co-receptor Endoglin in the molecular oscillator that regulates the hair follicle cycle
    Article Snippet: Proteins were resolved in a 7.5% SDS-PAGE system and transferred to PVDF membranes that were incubated with the indicated primary and secondary antibodies. .. For RNA extraction, E14 cell cultures coming from the treatments described above, or whole dorsal skin of at least three mice at each time point and genotype, RNeasy mini kit and RNase-Free DNase Set (both from Qiagen) were used. .. Skin tissue was homogenized using TriPureTM isolation Reagent (Roche), disaggregated and processed using scissors and a Polytron® homogenizer (PT 1200 E, Kinematica).

    Article Title: Hyperplasia of Interstitial Cells of Cajal in Sprouty Homolog 4 Deficient Mice
    Article Snippet: A minimum of three different RNA samples from P10 WT, Kit K641E/K641E and Spry4 KO mice antrum were used. .. Genomic DNA was removed using the RNase-Free DNase set (Qiagen).

    Sequencing:

    Article Title: Hyperplasia of Interstitial Cells of Cajal in Sprouty Homolog 4 Deficient Mice
    Article Snippet: Genomic DNA was removed using the RNase-Free DNase set (Qiagen). .. Genomic DNA was removed using the RNase-Free DNase set (Qiagen).

    Microscopy:

    Article Title: Laser capture microdissection in Ectocarpus siliculosus: the pathway to cell-specific transcriptomics in brown algae
    Article Snippet: Laser capture was performed using a Carl Zeiss PALM MicroBeam unit equipped with an AxioVert 200 microscope and a CryLas UV laser, and assisted by the PalmRobo 4.5 software. .. On column DNA digestion was carried out using Qiagen RNase-free DNase I set (#79254).

    Nuclease Assay:

    Article Title: Phenotype and Susceptibility to HIV-infection of CD4+ Th17 Cells in the Human Female Reproductive Tract
    Article Snippet: DNase digestion was done on-column with the RNase-Free DNase set (Qiagen). .. After RNA quantification, cDNA was generated with the iScript cDNA synthesis kit (Bio-Rad, Hercules, CA).

    Chloramphenicol Acetyltransferase Assay:

    Article Title: Repurposing Ponatinib as a Potent Agent against KIT Mutant Melanomas
    Article Snippet: Total RNA was extracted using RNeasy Mini Kit (Cat# 74106, Qiagen) following the manufacturer's instructions and checked for a RIN number to inspect RNA integrity by an Agilent Bioanalyzer 2100 (Agilent technologies, Santa Clara, CA, US). .. Qualified total RNA was further purified by RNAClean XP Kit (Cat A63987, Beckman Coulter,Inc.Kraemer Boulevard Brea, CA,USA)and RNase-Free DNase Set (Cat79254, QIAGEN, GmBH, Germany). .. The Illumina TruSeq RNA Sample Preparation Kit (Illumina) was used for library preparation.

    Article Title: The transcriptome sequencing and functional analysis of eyestalk ganglions in Chinese mitten crab (Eriocheir sinensis) treated with different photoperiods
    Article Snippet: Total RNA was extracted using RNAisoTM Plus (TaKaRa) according to manufacturer’s instructions. .. Qualified total RNA was further purified with a RNeasy micro kit (Cat74004, QIAGEN, GmBH, Germany) and RNase-Free DNase Set (Cat#79254, QIAGEN, GmBH, Germany). .. The RNA concentration and purity were determined using a Nanodrop2000, and RNA quantity was measured by denaturing formaldehyde agarose gel electrophoresis to examine integrity and measured with an Agilent Bioanalyzer 2100 (Agilent Technologies).

    Purification:

    Article Title: Repurposing Ponatinib as a Potent Agent against KIT Mutant Melanomas
    Article Snippet: Total RNA was extracted using RNeasy Mini Kit (Cat# 74106, Qiagen) following the manufacturer's instructions and checked for a RIN number to inspect RNA integrity by an Agilent Bioanalyzer 2100 (Agilent technologies, Santa Clara, CA, US). .. Qualified total RNA was further purified by RNAClean XP Kit (Cat A63987, Beckman Coulter,Inc.Kraemer Boulevard Brea, CA,USA)and RNase-Free DNase Set (Cat#79254, QIAGEN, GmBH, Germany). .. The Illumina TruSeq RNA Sample Preparation Kit (Illumina) was used for library preparation.

    Article Title: Stem Cells Derived from Lipoma and Adipose Tissue—Similar Mesenchymal Phenotype but Different Differentiation Capacity Governed by Distinct Molecular Signature
    Article Snippet: LDSCs and ADSCs at passage 2 (day 0 in differentiation assay), at day 21 in adipogenic differentiation assay and days 8 and 16 in osteogenic differentiation assay were placed in RNAlater® (Ambion, Life Technologies, Carlsbad, CA, USA) and stored at −80°C until RNA purification step. .. DNase I RNase-free set (Qiagen) was used for on-column digestion of residual genomic DNA, according to the manufacturer’s instructions.

    Article Title: Down-regulation of AR splice variants through XPO1 suppression contributes to the inhibition of prostate cancer progression
    Article Snippet: Anti-AR (N20) which recognizes both AR full length and AR splice variants (Santa Cruz, Santa Cruz, CA), anti-XPO1 (Santa Cruz), anti-FOXA1 (Novus Biologicals, Littleton, CO), anti-MED1 (Santa Cruz), anti-eIF4E (Cell Signaling, Danvers, MA), anti-Src (Cell Signaling), anti-p-Src(Tyr527) (Cell Signaling), anti-Lamin B (Invitrogen), and anti-β-actin (Sigma, St. Louis, MO) primary antibodies were used for Western Blot analysis. .. Total RNAs from PCa cell lines treated with SINE were extracted and purified by using the RNeasy Mini Kit and RNase-free DNase Set (QIAGEN, Valencia, CA) following the protocol provided by the manufacturer. .. Total RNAs from PCa tissues were isolated from formalin-fixed, paraffin-embedded tissue sections by using miRNeasy FFPE Kit (QIAGEN) following the protocol provided by the company.

    Article Title: Laser capture microdissection in Ectocarpus siliculosus: the pathway to cell-specific transcriptomics in brown algae
    Article Snippet: On column DNA digestion was carried out using Qiagen RNase-free DNase I set (#79254). .. On column DNA digestion was carried out using Qiagen RNase-free DNase I set (#79254).

    Article Title: Phenotype and Susceptibility to HIV-infection of CD4+ Th17 Cells in the Human Female Reproductive Tract
    Article Snippet: Total RNA was extracted from purified CD4+ T cells using the micro RNeasy kit (QIAGEN, Valencia, CA). .. DNase digestion was done on-column with the RNase-Free DNase set (Qiagen).

    Article Title: Time-resolved mapping of genetic interactions to model rewiring of signaling pathways
    Article Snippet: RNA was then purified from all samples according to manufacturer's standard instructions for spin column purification. .. An optional DNase digestion step was performed using the RNase-Free DNase Set (Qiagen).

    Article Title: The transcriptome sequencing and functional analysis of eyestalk ganglions in Chinese mitten crab (Eriocheir sinensis) treated with different photoperiods
    Article Snippet: Total RNA was extracted using RNAisoTM Plus (TaKaRa) according to manufacturer’s instructions. .. Qualified total RNA was further purified with a RNeasy micro kit (Cat#74004, QIAGEN, GmBH, Germany) and RNase-Free DNase Set (Cat#79254, QIAGEN, GmBH, Germany). .. The RNA concentration and purity were determined using a Nanodrop2000, and RNA quantity was measured by denaturing formaldehyde agarose gel electrophoresis to examine integrity and measured with an Agilent Bioanalyzer 2100 (Agilent Technologies).

    Article Title: Definition of the ?W Regulon of Bacillus subtilis in the Absence of Stress
    Article Snippet: Precipitated RNA was washed with 70% ethanol and dissolved in 100 µl of RNase free water. .. The isolated RNA was DNase-treated using the RNase-Free DNase Set (Qiagen) and purified using the RNA Clean-Up and Concentration Micro Kit (Norgen). .. RNA concentrations were measured using a Nanodrop-1000 spectrophotometer and RNA quality was assessed with the Agilent 2100 Bioanalyzer according to the manufacturer's instructions.

    Plasmid Preparation:

    Article Title: Overexpression of StDREB2 Transcription Factor Enhances Drought Stress Tolerance in Cotton (Gossypium barbadense L.)
    Article Snippet: Paragraph title: 2.2. Vector Construction and Cotton Transformation ... RNase-Free DNase Set (Qiagen) was then used to remove the contaminating DNA.

    Software:

    Article Title: Laser capture microdissection in Ectocarpus siliculosus: the pathway to cell-specific transcriptomics in brown algae
    Article Snippet: Laser capture was performed using a Carl Zeiss PALM MicroBeam unit equipped with an AxioVert 200 microscope and a CryLas UV laser, and assisted by the PalmRobo 4.5 software. .. On column DNA digestion was carried out using Qiagen RNase-free DNase I set (#79254).

    Real-time Polymerase Chain Reaction:

    Article Title: Stem Cells Derived from Lipoma and Adipose Tissue—Similar Mesenchymal Phenotype but Different Differentiation Capacity Governed by Distinct Molecular Signature
    Article Snippet: DNase I RNase-free set (Qiagen) was used for on-column digestion of residual genomic DNA, according to the manufacturer’s instructions. .. Reverse transcription was performed in the PCR thermal cycler SureCycler8800 (Agilent Technologies, Santa Clara, CA, USA).

    Article Title: Down-regulation of AR splice variants through XPO1 suppression contributes to the inhibition of prostate cancer progression
    Article Snippet: Total RNAs from PCa cell lines treated with SINE were extracted and purified by using the RNeasy Mini Kit and RNase-free DNase Set (QIAGEN, Valencia, CA) following the protocol provided by the manufacturer. .. The expression levels of AR, AR-v7, ARv567es, PSA, XPO1, FOXA1, MED1, Src, Vav3, Sam68, or UBE2C in selinexor or KPT-8602 treated or un-treated PCa cells and PCa tissues were analyzed by real-time RT-qPCR using High Capacity cDNA Reverse Transcription Kit and SYBR Green Master Mixture from Applied Biosystems (Waltham, MA).

    Article Title: Laser capture microdissection in Ectocarpus siliculosus: the pathway to cell-specific transcriptomics in brown algae
    Article Snippet: On column DNA digestion was carried out using Qiagen RNase-free DNase I set (#79254). .. On column DNA digestion was carried out using Qiagen RNase-free DNase I set (#79254).

    Article Title: Phenotype and Susceptibility to HIV-infection of CD4+ Th17 Cells in the Human Female Reproductive Tract
    Article Snippet: Paragraph title: RNA isolation and real time PCR ... DNase digestion was done on-column with the RNase-Free DNase set (Qiagen).

    Article Title: Time-resolved mapping of genetic interactions to model rewiring of signaling pathways
    Article Snippet: Paragraph title: qPCR analysis ... An optional DNase digestion step was performed using the RNase-Free DNase Set (Qiagen).

    Article Title: Hyperplasia of Interstitial Cells of Cajal in Sprouty Homolog 4 Deficient Mice
    Article Snippet: Paragraph title: Real time quantitative PCR (qPCR) ... Genomic DNA was removed using the RNase-Free DNase set (Qiagen).

    Article Title: Effects of Obesity and Metabolic Syndrome on Steroidogenesis and Folliculogenesis in the Female Ossabaw Mini-Pig
    Article Snippet: Paragraph title: Quantitative real time PCR ... Total RNA was extracted from cell aliquots of 100,000 cells using the RNeasy Mini Kit (Qiagen Inc) and an on-column DNase treatment (RNase-free DNase Set, Qiagen Inc).

    RNA Extraction:

    Article Title: Laser capture microdissection in Ectocarpus siliculosus: the pathway to cell-specific transcriptomics in brown algae
    Article Snippet: The PicoPure RNA extraction kit from Life Technologies (#KIT0204) was used to extract RNA. .. On column DNA digestion was carried out using Qiagen RNase-free DNase I set (#79254).

    Article Title: A role for the Tgf- β/Bmp co-receptor Endoglin in the molecular oscillator that regulates the hair follicle cycle
    Article Snippet: Proteins were resolved in a 7.5% SDS-PAGE system and transferred to PVDF membranes that were incubated with the indicated primary and secondary antibodies. .. For RNA extraction, E14 cell cultures coming from the treatments described above, or whole dorsal skin of at least three mice at each time point and genotype, RNeasy mini kit and RNase-Free DNase Set (both from Qiagen) were used. .. Skin tissue was homogenized using TriPureTM isolation Reagent (Roche), disaggregated and processed using scissors and a Polytron® homogenizer (PT 1200 E, Kinematica).

    Article Title: Duplicated Leptin Receptors in Two Species of Eel Bring New Insights into the Evolution of the Leptin System in Vertebrates
    Article Snippet: Paragraph title: RNA extraction ... A deoxyribonuclease I treatment, using the Qiagen RNase-free DNase Set, was applied during the procedure, according to the manufacturer instructions.

    Homogenization:

    Article Title: Duplicated Leptin Receptors in Two Species of Eel Bring New Insights into the Evolution of the Leptin System in Vertebrates
    Article Snippet: Extraction of total RNA was performed using mechanical homogenization (TissueLyser II, Qiagen, Hilden, Germany). .. A deoxyribonuclease I treatment, using the Qiagen RNase-free DNase Set, was applied during the procedure, according to the manufacturer instructions.

    Spectrophotometry:

    Article Title: Campylobacter coli From Retail Liver and Meat Products Is More Aerotolerant Than Campylobacter jejuni
    Article Snippet: After washing and elution steps according to manufacturer's protocol, RNA samples were further subjected to DNA digestion in solution using RNase Free DNase set (Qiagen). .. After washing and elution steps according to manufacturer's protocol, RNA samples were further subjected to DNA digestion in solution using RNase Free DNase set (Qiagen).

    Article Title: Laser capture microdissection in Ectocarpus siliculosus: the pathway to cell-specific transcriptomics in brown algae
    Article Snippet: On column DNA digestion was carried out using Qiagen RNase-free DNase I set (#79254). .. On column DNA digestion was carried out using Qiagen RNase-free DNase I set (#79254).

    Concentration Assay:

    Article Title: Definition of the ?W Regulon of Bacillus subtilis in the Absence of Stress
    Article Snippet: Precipitated RNA was washed with 70% ethanol and dissolved in 100 µl of RNase free water. .. The isolated RNA was DNase-treated using the RNase-Free DNase Set (Qiagen) and purified using the RNA Clean-Up and Concentration Micro Kit (Norgen). .. RNA concentrations were measured using a Nanodrop-1000 spectrophotometer and RNA quality was assessed with the Agilent 2100 Bioanalyzer according to the manufacturer's instructions.

    Construct:

    Article Title: Overexpression of StDREB2 Transcription Factor Enhances Drought Stress Tolerance in Cotton (Gossypium barbadense L.)
    Article Snippet: RNase-Free DNase Set (Qiagen) was then used to remove the contaminating DNA. .. Briefly, StDREB2 cDNA was inserted into a pMDC32 vector (Invitrogen).

    Differentiation Assay:

    Article Title: Stem Cells Derived from Lipoma and Adipose Tissue—Similar Mesenchymal Phenotype but Different Differentiation Capacity Governed by Distinct Molecular Signature
    Article Snippet: LDSCs and ADSCs at passage 2 (day 0 in differentiation assay), at day 21 in adipogenic differentiation assay and days 8 and 16 in osteogenic differentiation assay were placed in RNAlater® (Ambion, Life Technologies, Carlsbad, CA, USA) and stored at −80°C until RNA purification step. .. DNase I RNase-free set (Qiagen) was used for on-column digestion of residual genomic DNA, according to the manufacturer’s instructions.

    Lysis:

    Article Title: Campylobacter coli From Retail Liver and Meat Products Is More Aerotolerant Than Campylobacter jejuni
    Article Snippet: For total RNA isolation, 2 ml RNA protect bacteria reagent (Qiagen) was added to 1 ml of bacterial culture and vortexed for 5 s followed by incubation at room temperature for 5 min. Bacterial cell was harvested by centrifugation at 5,000 × g for 5 min at 4°C and cell pellet was resuspended in 700 μl of lysis buffer (RLT buffer, Qiagen) by vortexing for 10 s. Bacterial cell lysis was done by vortexing vigorously for 5 min in 2 ml safe lock tube containing ~30 mg acid washed glass beads (212–300 μm, Sigma, G1277). .. After washing and elution steps according to manufacturer's protocol, RNA samples were further subjected to DNA digestion in solution using RNase Free DNase set (Qiagen).

    Article Title: Definition of the ?W Regulon of Bacillus subtilis in the Absence of Stress
    Article Snippet: The frozen powder was resuspended in 4 mL prewarmed (50°C) lysis solution (4 M guanidine thiocyanate, 25 mM sodium acetate [pH 5.2], 0.5% N -laurylsarcosinate [wt/vol]) and immediately frozen in liquid nitrogen. .. The isolated RNA was DNase-treated using the RNase-Free DNase Set (Qiagen) and purified using the RNA Clean-Up and Concentration Micro Kit (Norgen).

    Article Title: Intrinsic terminators in Mycoplasma hyopneumoniae transcription
    Article Snippet: For cell lyses, 0.7 ml of RNeasy Lysis Buffer (RLT buffer) in the presence of 0.134 M of β-mercaptoethanol was used per cultivation flask. .. The purification was performed according to the manufacturer’s instructions, with on-column DNaseI digestion using the RNase-Free DNase Set (Qiagen, Germany) and a second round of treatment with DNase I (Fermentas, USA).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Qiagen rnase free dnase
    Enzymatic digestion of decellularized ECM scaffolds releases small RNA molecules. ( A ) Nucleic acid extracted from untreated UBM (no digest) and pepsin-, proteinase K–, or collagenase-treated UBM was exposed to <t>RNase</t> A, <t>DNase</t> I, or no-nuclease treatment (control). ( B ) Electropherogram depicting the small RNA pattern of nucleic acid in fluorescence units (FU) before (top panel) and after (bottom panel) DNase I treatment. ( C ) Electropherogram depicting small RNA pattern from the indicated samples in FU. ( D ) A subset of nucleic molecules in biologic scaffolds is protected from nuclease degradation.
    Rnase Free Dnase, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 355 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rnase free dnase/product/Qiagen
    Average 99 stars, based on 355 article reviews
    Price from $9.99 to $1999.99
    rnase free dnase - by Bioz Stars, 2019-10
    99/100 stars
      Buy from Supplier

    99
    Qiagen ie mater transgenic mice
    Effect of NTx with regard to development of anti-MATER and ANA in wild-type and IE-Mater <t>transgenic</t> mice. A, Anti-MATER antibodies: Based on immunoblotting assay, 77% of the thymectomized wild-type (n = 17) and 24% of the thymectomized IE-Mater transgenic
    Ie Mater Transgenic Mice, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ie mater transgenic mice/product/Qiagen
    Average 99 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    ie mater transgenic mice - by Bioz Stars, 2019-10
    99/100 stars
      Buy from Supplier

    Image Search Results


    Enzymatic digestion of decellularized ECM scaffolds releases small RNA molecules. ( A ) Nucleic acid extracted from untreated UBM (no digest) and pepsin-, proteinase K–, or collagenase-treated UBM was exposed to RNase A, DNase I, or no-nuclease treatment (control). ( B ) Electropherogram depicting the small RNA pattern of nucleic acid in fluorescence units (FU) before (top panel) and after (bottom panel) DNase I treatment. ( C ) Electropherogram depicting small RNA pattern from the indicated samples in FU. ( D ) A subset of nucleic molecules in biologic scaffolds is protected from nuclease degradation.

    Journal: Science Advances

    Article Title: Matrix-bound nanovesicles within ECM bioscaffolds

    doi: 10.1126/sciadv.1600502

    Figure Lengend Snippet: Enzymatic digestion of decellularized ECM scaffolds releases small RNA molecules. ( A ) Nucleic acid extracted from untreated UBM (no digest) and pepsin-, proteinase K–, or collagenase-treated UBM was exposed to RNase A, DNase I, or no-nuclease treatment (control). ( B ) Electropherogram depicting the small RNA pattern of nucleic acid in fluorescence units (FU) before (top panel) and after (bottom panel) DNase I treatment. ( C ) Electropherogram depicting small RNA pattern from the indicated samples in FU. ( D ) A subset of nucleic molecules in biologic scaffolds is protected from nuclease degradation.

    Article Snippet: RNase-free DNase was obtained from Qiagen.

    Techniques: Fluorescence

    Extracellular vesicle internalization and transfer of GFP mRNA ( A ) Super resolution 3D reconstruction of GFP+ mRPC following 24 h incubation with PKH26 labeled extracellular vesicles. Red vesicles are visibly localized near the cell surface and within cytoplasm. In the XZ axis, GFP (green), EVs (red) and nuclei (blue, DAPI). ( B ) same as ( A ) with GFP (FITC) channel removed to increase visibility of PKH26 (TRITC) labeled EVs. Each panel contains three cross-sectional views (xy, xz, and yz). Scale: 5 µm. ( C ) RT-PCR analysis of GFP mRNA transfer between GFP+ mRPCs and non-GFP hRPCs. Non-GFP hRPCs served as negative control; GFP+ mRPCs served as postive control. GAPDH served as the internal control gene. EVs were treated using an RNase-Free DNase Set to remove DNA comtamination before cDNA synthesis. ( D ) Intensities of RT-PCR images were measured with ImageJ software and normalized to GAPDH. Relative levels of hRPC GFP after transfer of EVs is significantly higher than negative control.

    Journal: Scientific Reports

    Article Title: Retinal progenitor cells release extracellular vesicles containing developmental transcription factors, microRNA and membrane proteins

    doi: 10.1038/s41598-018-20421-1

    Figure Lengend Snippet: Extracellular vesicle internalization and transfer of GFP mRNA ( A ) Super resolution 3D reconstruction of GFP+ mRPC following 24 h incubation with PKH26 labeled extracellular vesicles. Red vesicles are visibly localized near the cell surface and within cytoplasm. In the XZ axis, GFP (green), EVs (red) and nuclei (blue, DAPI). ( B ) same as ( A ) with GFP (FITC) channel removed to increase visibility of PKH26 (TRITC) labeled EVs. Each panel contains three cross-sectional views (xy, xz, and yz). Scale: 5 µm. ( C ) RT-PCR analysis of GFP mRNA transfer between GFP+ mRPCs and non-GFP hRPCs. Non-GFP hRPCs served as negative control; GFP+ mRPCs served as postive control. GAPDH served as the internal control gene. EVs were treated using an RNase-Free DNase Set to remove DNA comtamination before cDNA synthesis. ( D ) Intensities of RT-PCR images were measured with ImageJ software and normalized to GAPDH. Relative levels of hRPC GFP after transfer of EVs is significantly higher than negative control.

    Article Snippet: DNA contamination was removed using an RNase-Free DNase Set (Qiagen). cDNA was synthesized using equal amounts of RNA samples (800 ng), according to the AMV First Strand cDNA Synthesis Kit instructions (NEB Biolabs). β-actin and GAPDH were used as a housekeeping gene controls for mRNA analysis , .

    Techniques: Incubation, Labeling, Reverse Transcription Polymerase Chain Reaction, Negative Control, Software

    Aar modulates DNA-binding properties of H-NS. orf2223  and  orf3928  probes (Panel A) were incubated with different concentration of H-NS protein and analyzed by EMSA (Panel B). H-NS binding sites are indicated in red. In a second experiment, the samples were incubated with either H-NS, Aar or both proteins and analyzed by EMSA (Panel C). In parallel,  proV  was incubated with H-NS in either presence or absence of Aar. The presence of Aar abolished the H-NS-DNA interaction (panel D, white arrows, lanes 2, 3, 5, 6). A similar approach was used to evaluate the effect of using increasing concentrations of Aar (0.8–12.8 μM) (panel E, lanes 3 to 7). DNA probe and H-NS samples were incubated in either presence or absence of Aar and treated with DNase I. Incubation of samples with Aar (3.2 μM) disrupted DNA protection and increased DNase I effect on  proV  probe (panel F, black arrows, lanes 6, 7, 13, 14).

    Journal: PLoS Pathogens

    Article Title: The AraC Negative Regulator family modulates the activity of histone-like proteins in pathogenic bacteria

    doi: 10.1371/journal.ppat.1006545

    Figure Lengend Snippet: Aar modulates DNA-binding properties of H-NS. orf2223 and orf3928 probes (Panel A) were incubated with different concentration of H-NS protein and analyzed by EMSA (Panel B). H-NS binding sites are indicated in red. In a second experiment, the samples were incubated with either H-NS, Aar or both proteins and analyzed by EMSA (Panel C). In parallel, proV was incubated with H-NS in either presence or absence of Aar. The presence of Aar abolished the H-NS-DNA interaction (panel D, white arrows, lanes 2, 3, 5, 6). A similar approach was used to evaluate the effect of using increasing concentrations of Aar (0.8–12.8 μM) (panel E, lanes 3 to 7). DNA probe and H-NS samples were incubated in either presence or absence of Aar and treated with DNase I. Incubation of samples with Aar (3.2 μM) disrupted DNA protection and increased DNase I effect on proV probe (panel F, black arrows, lanes 6, 7, 13, 14).

    Article Snippet: For the DNase I protection assays, after protein-DNA complex formation, the samples were incubated with 25 ηg of DNase I (RNase-free DNase set) (Qiagen) for 30 min.

    Techniques: Binding Assay, Incubation, Concentration Assay

    Effect of NTx with regard to development of anti-MATER and ANA in wild-type and IE-Mater transgenic mice. A, Anti-MATER antibodies: Based on immunoblotting assay, 77% of the thymectomized wild-type (n = 17) and 24% of the thymectomized IE-Mater transgenic

    Journal:

    Article Title: Autoimmune Oophoritis with Multiple Molecular Targets Mitigated by Transgenic Expression of Mater

    doi: 10.1210/en.2011-0022

    Figure Lengend Snippet: Effect of NTx with regard to development of anti-MATER and ANA in wild-type and IE-Mater transgenic mice. A, Anti-MATER antibodies: Based on immunoblotting assay, 77% of the thymectomized wild-type (n = 17) and 24% of the thymectomized IE-Mater transgenic

    Article Snippet: RNA was extracted from thymus, spleen, and ovaries of wild-type (C57BL/6J) and IE-Mater transgenic mice (QIAGEN RNeasy Mini Kit with RNase-Free DNase Set) followed by reverse transcription (RT) (QIAGEN Omniscript RT Kit) of the purified mRNA with oligo dT18 primers.

    Techniques: Transgenic Assay, Mouse Assay

    Incidence of autoimmune oophoritis after NTx in wild-type and IE-Mater transgenic mice. A, Histopathology of autoimmune oophoritis after NTx. Representative sections of ovarian histology with hematoxylin and eosin staining are shown for sham-operated

    Journal:

    Article Title: Autoimmune Oophoritis with Multiple Molecular Targets Mitigated by Transgenic Expression of Mater

    doi: 10.1210/en.2011-0022

    Figure Lengend Snippet: Incidence of autoimmune oophoritis after NTx in wild-type and IE-Mater transgenic mice. A, Histopathology of autoimmune oophoritis after NTx. Representative sections of ovarian histology with hematoxylin and eosin staining are shown for sham-operated

    Article Snippet: RNA was extracted from thymus, spleen, and ovaries of wild-type (C57BL/6J) and IE-Mater transgenic mice (QIAGEN RNeasy Mini Kit with RNase-Free DNase Set) followed by reverse transcription (RT) (QIAGEN Omniscript RT Kit) of the purified mRNA with oligo dT18 primers.

    Techniques: Transgenic Assay, Mouse Assay, Histopathology, Staining

    Antioocyte autoantibodies in wild-type and IE-Mater transgenic mice. A, Incidence. Based on immunofluorescence on frozen ovarian sections (B) and confirmation with the immunoblotting assay, the sera from 82% of the wild-type (n = 17) and 28% of the IE-Mater

    Journal:

    Article Title: Autoimmune Oophoritis with Multiple Molecular Targets Mitigated by Transgenic Expression of Mater

    doi: 10.1210/en.2011-0022

    Figure Lengend Snippet: Antioocyte autoantibodies in wild-type and IE-Mater transgenic mice. A, Incidence. Based on immunofluorescence on frozen ovarian sections (B) and confirmation with the immunoblotting assay, the sera from 82% of the wild-type (n = 17) and 28% of the IE-Mater

    Article Snippet: RNA was extracted from thymus, spleen, and ovaries of wild-type (C57BL/6J) and IE-Mater transgenic mice (QIAGEN RNeasy Mini Kit with RNase-Free DNase Set) followed by reverse transcription (RT) (QIAGEN Omniscript RT Kit) of the purified mRNA with oligo dT18 primers.

    Techniques: Transgenic Assay, Mouse Assay, Immunofluorescence