rnase free dnase set  (Qiagen)


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    Name:
    RNase-Free DNase Set
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    Catalog Number:
    79254
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    Score:
    85
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    Structured Review

    Qiagen rnase free dnase set
    Extracellular vesicle internalization and transfer of GFP mRNA ( A ) Super resolution 3D reconstruction of GFP+ mRPC following 24 h incubation with PKH26 labeled extracellular vesicles. Red vesicles are visibly localized near the cell surface and within cytoplasm. In the XZ axis, GFP (green), EVs (red) and nuclei (blue, DAPI). ( B ) same as ( A ) with GFP (FITC) channel removed to increase visibility of PKH26 (TRITC) labeled EVs. Each panel contains three cross-sectional views (xy, xz, and yz). Scale: 5 µm. ( C ) RT-PCR analysis of GFP mRNA transfer between GFP+ mRPCs and non-GFP hRPCs. Non-GFP hRPCs served as negative control; GFP+ mRPCs served as postive control. GAPDH served as the internal control gene. EVs were treated using an <t>RNase-Free</t> <t>DNase</t> Set to remove DNA comtamination before cDNA synthesis. ( D ) Intensities of RT-PCR images were measured with ImageJ software and normalized to GAPDH. Relative levels of hRPC GFP after transfer of EVs is significantly higher than negative control.

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    Related Products / Commonly Used Together

    rneasy mini

    Images

    1) Product Images from "Retinal progenitor cells release extracellular vesicles containing developmental transcription factors, microRNA and membrane proteins"

    Article Title: Retinal progenitor cells release extracellular vesicles containing developmental transcription factors, microRNA and membrane proteins

    Journal: Scientific Reports

    doi: 10.1038/s41598-018-20421-1

    Extracellular vesicle internalization and transfer of GFP mRNA ( A ) Super resolution 3D reconstruction of GFP+ mRPC following 24 h incubation with PKH26 labeled extracellular vesicles. Red vesicles are visibly localized near the cell surface and within cytoplasm. In the XZ axis, GFP (green), EVs (red) and nuclei (blue, DAPI). ( B ) same as ( A ) with GFP (FITC) channel removed to increase visibility of PKH26 (TRITC) labeled EVs. Each panel contains three cross-sectional views (xy, xz, and yz). Scale: 5 µm. ( C ) RT-PCR analysis of GFP mRNA transfer between GFP+ mRPCs and non-GFP hRPCs. Non-GFP hRPCs served as negative control; GFP+ mRPCs served as postive control. GAPDH served as the internal control gene. EVs were treated using an RNase-Free DNase Set to remove DNA comtamination before cDNA synthesis. ( D ) Intensities of RT-PCR images were measured with ImageJ software and normalized to GAPDH. Relative levels of hRPC GFP after transfer of EVs is significantly higher than negative control.
    Figure Legend Snippet: Extracellular vesicle internalization and transfer of GFP mRNA ( A ) Super resolution 3D reconstruction of GFP+ mRPC following 24 h incubation with PKH26 labeled extracellular vesicles. Red vesicles are visibly localized near the cell surface and within cytoplasm. In the XZ axis, GFP (green), EVs (red) and nuclei (blue, DAPI). ( B ) same as ( A ) with GFP (FITC) channel removed to increase visibility of PKH26 (TRITC) labeled EVs. Each panel contains three cross-sectional views (xy, xz, and yz). Scale: 5 µm. ( C ) RT-PCR analysis of GFP mRNA transfer between GFP+ mRPCs and non-GFP hRPCs. Non-GFP hRPCs served as negative control; GFP+ mRPCs served as postive control. GAPDH served as the internal control gene. EVs were treated using an RNase-Free DNase Set to remove DNA comtamination before cDNA synthesis. ( D ) Intensities of RT-PCR images were measured with ImageJ software and normalized to GAPDH. Relative levels of hRPC GFP after transfer of EVs is significantly higher than negative control.

    Techniques Used: Incubation, Labeling, Reverse Transcription Polymerase Chain Reaction, Negative Control, Software

    2) Product Images from "MicroRNAs Circulate in the Hemolymph of Drosophila and Accumulate Relative to Tissue microRNAs in an Age-Dependent Manner"

    Article Title: MicroRNAs Circulate in the Hemolymph of Drosophila and Accumulate Relative to Tissue microRNAs in an Age-Dependent Manner

    Journal: Genomics Insights

    doi: 10.4137/GEI.S38147

    Presence of stable miRNAs in Drosophila melanogaster hemolymph. ( A , B ) Clear HL droplets extruded from fly head and thorax. ( C – F ) Real-time qPCR amplification of selected HL miRNAs and mRNAs. Total RNA including small RNA was extracted from HL samples and analyzed with qPCR to measure the levels of miRNAs and mRNAs. The y -axis represents the relative fluorescence units (RFU) in a semi-log scale. The x -axis represents the cycle at which fluorescence was detected above an automatically determined threshold. ( C ) Amplification plots for miR-14, miR-8, and miR-184 measured in a representative HL sample. ( D ) Amplification plots for miR-14, let-7, bantam, and spiked-in synthetic C. elegans cel-miR-39 RNA in representative HL sample. ( E ) The amplification plots for tubulin, actin, and gapdh mRNAs determined by qPCR using total RNA from HL and S2 cells. Sample from S2 cells are used as a positive control for detecting Drosophila mRNAs by qPCR. The amplification curves of all three mRNAs are superimposed on one another, reflecting the presence of similar amounts of these mRNA in S2 cells. Amplification curves from HL samples show that fluorescent products appear after about 30 cycles, reflecting the significantly lower abundance of these mRNAs in HL relative to S2 cells. ( F ) The cycle threshold (Ct) fold-change of selected miRNA amplified in the absence or presence of RNase A and DNase I. Total RNA was extracted from HL samples spiked with 10 fmoles of cel-miR-39 RNA. The x -axis represents the ratio of raw Ct values from control samples divided by raw Ct values from samples incubated with RNase A and DNase I. The significantly higher magnitude of the Ct fold change of the spiked-in synthetic miRNA relative to those of the miRNA indicates that the HL-miRNA are present in nuclease-resistant, stable form.
    Figure Legend Snippet: Presence of stable miRNAs in Drosophila melanogaster hemolymph. ( A , B ) Clear HL droplets extruded from fly head and thorax. ( C – F ) Real-time qPCR amplification of selected HL miRNAs and mRNAs. Total RNA including small RNA was extracted from HL samples and analyzed with qPCR to measure the levels of miRNAs and mRNAs. The y -axis represents the relative fluorescence units (RFU) in a semi-log scale. The x -axis represents the cycle at which fluorescence was detected above an automatically determined threshold. ( C ) Amplification plots for miR-14, miR-8, and miR-184 measured in a representative HL sample. ( D ) Amplification plots for miR-14, let-7, bantam, and spiked-in synthetic C. elegans cel-miR-39 RNA in representative HL sample. ( E ) The amplification plots for tubulin, actin, and gapdh mRNAs determined by qPCR using total RNA from HL and S2 cells. Sample from S2 cells are used as a positive control for detecting Drosophila mRNAs by qPCR. The amplification curves of all three mRNAs are superimposed on one another, reflecting the presence of similar amounts of these mRNA in S2 cells. Amplification curves from HL samples show that fluorescent products appear after about 30 cycles, reflecting the significantly lower abundance of these mRNAs in HL relative to S2 cells. ( F ) The cycle threshold (Ct) fold-change of selected miRNA amplified in the absence or presence of RNase A and DNase I. Total RNA was extracted from HL samples spiked with 10 fmoles of cel-miR-39 RNA. The x -axis represents the ratio of raw Ct values from control samples divided by raw Ct values from samples incubated with RNase A and DNase I. The significantly higher magnitude of the Ct fold change of the spiked-in synthetic miRNA relative to those of the miRNA indicates that the HL-miRNA are present in nuclease-resistant, stable form.

    Techniques Used: Real-time Polymerase Chain Reaction, Amplification, Fluorescence, Positive Control, Incubation

    3) Product Images from "Transparent DNA/RNA Co-extraction Workflow Protocol Suitable for Inhibitor-Rich Environmental Samples That Focuses on Complete DNA Removal for Transcriptomic Analyses"

    Article Title: Transparent DNA/RNA Co-extraction Workflow Protocol Suitable for Inhibitor-Rich Environmental Samples That Focuses on Complete DNA Removal for Transcriptomic Analyses

    Journal: Frontiers in Microbiology

    doi: 10.3389/fmicb.2016.01588

    Suggested DNA/RNA co-extraction workflow for environmental samples, with stronger emphasis on thorough purification prior to all enzymatic steps (including DNase digestion). Optional steps are indicated by dotted arrows. Note that RNase digestion (between Extracts II and III) may be necessary for better results downstream, but may be omitted as a separate step (in the current study, RNase is present in the qPCR mix). (A) Pre-lysis inhibitor removal is only advisable if quick methods are used, or if mRNA is not the target molecule (lengthy inhibitor removal procedures compromise RNA integrity). (B) Various methods may be used, such as phenol/chloroform procedures or nucleic acid precipitation. (C) This purification step should target the removal of enzymatic-inhibitors (e.g., humic/fulvic acids and polyphenolics). (D) Purification of partially digested RNA extracts with residual genomic DNA aids in the removal of enduring inhibitors, prior to further digestion. (E) Stringent and well-documented quality control via rigorous and sensitive detection (preferably quantitative methods) is necessary to detect residual amplifiable gDNA prior to reverse transcription.
    Figure Legend Snippet: Suggested DNA/RNA co-extraction workflow for environmental samples, with stronger emphasis on thorough purification prior to all enzymatic steps (including DNase digestion). Optional steps are indicated by dotted arrows. Note that RNase digestion (between Extracts II and III) may be necessary for better results downstream, but may be omitted as a separate step (in the current study, RNase is present in the qPCR mix). (A) Pre-lysis inhibitor removal is only advisable if quick methods are used, or if mRNA is not the target molecule (lengthy inhibitor removal procedures compromise RNA integrity). (B) Various methods may be used, such as phenol/chloroform procedures or nucleic acid precipitation. (C) This purification step should target the removal of enzymatic-inhibitors (e.g., humic/fulvic acids and polyphenolics). (D) Purification of partially digested RNA extracts with residual genomic DNA aids in the removal of enduring inhibitors, prior to further digestion. (E) Stringent and well-documented quality control via rigorous and sensitive detection (preferably quantitative methods) is necessary to detect residual amplifiable gDNA prior to reverse transcription.

    Techniques Used: Environmental Sampling, Purification, Real-time Polymerase Chain Reaction, Lysis

    Related Articles

    Centrifugation:

    Article Title: Campylobacter coli From Retail Liver and Meat Products Is More Aerotolerant Than Campylobacter jejuni
    Article Snippet: For total RNA isolation, 2 ml RNA protect bacteria reagent (Qiagen) was added to 1 ml of bacterial culture and vortexed for 5 s followed by incubation at room temperature for 5 min. Bacterial cell was harvested by centrifugation at 5,000 × g for 5 min at 4°C and cell pellet was resuspended in 700 μl of lysis buffer (RLT buffer, Qiagen) by vortexing for 10 s. Bacterial cell lysis was done by vortexing vigorously for 5 min in 2 ml safe lock tube containing ~30 mg acid washed glass beads (212–300 μm, Sigma, G1277). .. After washing and elution steps according to manufacturer's protocol, RNA samples were further subjected to DNA digestion in solution using RNase Free DNase set (Qiagen).

    Amplification:

    Article Title: Hyperplasia of Interstitial Cells of Cajal in Sprouty Homolog 4 Deficient Mice
    Article Snippet: Genomic DNA was removed using the RNase-Free DNase set (Qiagen). .. Genomic DNA was removed using the RNase-Free DNase set (Qiagen).

    Article Title: Laser capture microdissection in Ectocarpus siliculosus: the pathway to cell-specific transcriptomics in brown algae
    Article Snippet: On column DNA digestion was carried out using Qiagen RNase-free DNase I set (#79254). .. On column DNA digestion was carried out using Qiagen RNase-free DNase I set (#79254).

    Article Title: Phenotype and Susceptibility to HIV-infection of CD4+ Th17 Cells in the Human Female Reproductive Tract
    Article Snippet: DNase digestion was done on-column with the RNase-Free DNase set (Qiagen). .. DNase digestion was done on-column with the RNase-Free DNase set (Qiagen).

    Article Title: Enhanced Arabidopsis pattern-triggered immunity by overexpression of cysteine-rich receptor-like kinases
    Article Snippet: Genomic DNA contaminations were removed using Qiagen RNase-Free DNase Set. .. The cDNA was then diluted 5-times before reverse transcription-PCR (RT-PCR) or quantitative RT-PCR (qRT-PCR) analyses.

    Synthesized:

    Article Title: Stem Cells Derived from Lipoma and Adipose Tissue—Similar Mesenchymal Phenotype but Different Differentiation Capacity Governed by Distinct Molecular Signature
    Article Snippet: DNase I RNase-free set (Qiagen) was used for on-column digestion of residual genomic DNA, according to the manufacturer’s instructions. .. Reverse transcription was performed in the PCR thermal cycler SureCycler8800 (Agilent Technologies, Santa Clara, CA, USA).

    Quantitative RT-PCR:

    Article Title: Effects of Obesity and Metabolic Syndrome on Steroidogenesis and Folliculogenesis in the Female Ossabaw Mini-Pig
    Article Snippet: Total RNA was extracted from cell aliquots of 100,000 cells using the RNeasy Mini Kit (Qiagen Inc) and an on-column DNase treatment (RNase-free DNase Set, Qiagen Inc). .. Total RNA was extracted from cell aliquots of 100,000 cells using the RNeasy Mini Kit (Qiagen Inc) and an on-column DNase treatment (RNase-free DNase Set, Qiagen Inc).

    Article Title: Down-regulation of AR splice variants through XPO1 suppression contributes to the inhibition of prostate cancer progression
    Article Snippet: Paragraph title: RNA isolation and mRNA real-time RT-qPCR ... Total RNAs from PCa cell lines treated with SINE were extracted and purified by using the RNeasy Mini Kit and RNase-free DNase Set (QIAGEN, Valencia, CA) following the protocol provided by the manufacturer.

    Article Title: Enhanced Arabidopsis pattern-triggered immunity by overexpression of cysteine-rich receptor-like kinases
    Article Snippet: Genomic DNA contaminations were removed using Qiagen RNase-Free DNase Set. .. Genomic DNA contaminations were removed using Qiagen RNase-Free DNase Set.

    SYBR Green Assay:

    Article Title: Hyperplasia of Interstitial Cells of Cajal in Sprouty Homolog 4 Deficient Mice
    Article Snippet: Genomic DNA was removed using the RNase-Free DNase set (Qiagen). .. Genomic DNA was removed using the RNase-Free DNase set (Qiagen).

    Article Title: Down-regulation of AR splice variants through XPO1 suppression contributes to the inhibition of prostate cancer progression
    Article Snippet: Total RNAs from PCa cell lines treated with SINE were extracted and purified by using the RNeasy Mini Kit and RNase-free DNase Set (QIAGEN, Valencia, CA) following the protocol provided by the manufacturer. .. Total RNAs from PCa cell lines treated with SINE were extracted and purified by using the RNeasy Mini Kit and RNase-free DNase Set (QIAGEN, Valencia, CA) following the protocol provided by the manufacturer.

    Random Hexamer Labeling:

    Article Title: Comparative Transcriptome and iTRAQ Proteome Analyses Reveal the Mechanisms of Diapause in Aphidius gifuensis Ashmead (Hymenoptera: Aphidiidae)
    Article Snippet: Qualified total RNA was further purified with the RNeasy micro kit (Qiagen, Hilden, Germany) and the RNase-Free DNase Set (Qiagen, Hilden, Germany). .. Total RNA was extracted and oligo (dT) magnetic beads and fragmentation buffer were used to generate short fragments of the isolated poly(A) mRNA.

    Expressing:

    Article Title: Analysis of ADP-glucose pyrophosphorylase expression during turion formation induced by abscisic acid in Spirodela polyrhiza (greater duckweed)
    Article Snippet: Considering that only daughter fronds shorter than 0.7 mm in length respond to ABA treatment and undergo turion formation after ABA treatment [ ], developing turions only with specific sizes were collected at their developmental stages after 0 (no ABA addition), 1, 2, 3, 5, 7 days of ABA treatment, respectively, for quantification of APL gene expression (Table ). .. The on-column DNase I was used to remove contaminating genomic DNA (Qiagen, 79254).

    Article Title: Effects of Obesity and Metabolic Syndrome on Steroidogenesis and Folliculogenesis in the Female Ossabaw Mini-Pig
    Article Snippet: Total RNA was extracted from cell aliquots of 100,000 cells using the RNeasy Mini Kit (Qiagen Inc) and an on-column DNase treatment (RNase-free DNase Set, Qiagen Inc). .. Total RNA was extracted from cell aliquots of 100,000 cells using the RNeasy Mini Kit (Qiagen Inc) and an on-column DNase treatment (RNase-free DNase Set, Qiagen Inc).

    Article Title: The Chromosomal Proteins JIL-1 and Z4/Putzig Regulate the Telomeric Chromatin in Drosophila melanogaster
    Article Snippet: RNase Free DNase Set (Qiagen) was used to remove genomic DNA contaminations as follows: one on column during the extraction accordingly to manufacturer's protocol, and two in solution for 2 hours at 37°C. .. RNA was cleaned by precipitation and its quality was assessed using NanoDrop spectrophotometry.

    Article Title: FGF21 increases water intake, urine output and blood pressure in rats
    Article Snippet: Paragraph title: Gene expression ... RNA was extracted from cells using the Qiagen RNeasy 96 kit (Qiagen 74181) including a DNaseI digestion step (Qiagen 79254).

    Article Title: Repurposing Ponatinib as a Potent Agent against KIT Mutant Melanomas
    Article Snippet: Qualified total RNA was further purified by RNAClean XP Kit (Cat A63987, Beckman Coulter,Inc.Kraemer Boulevard Brea, CA,USA)and RNase-Free DNase Set (Cat#79254, QIAGEN, GmBH, Germany). .. Qualified total RNA was further purified by RNAClean XP Kit (Cat A63987, Beckman Coulter,Inc.Kraemer Boulevard Brea, CA,USA)and RNase-Free DNase Set (Cat#79254, QIAGEN, GmBH, Germany).

    Article Title: Down-regulation of AR splice variants through XPO1 suppression contributes to the inhibition of prostate cancer progression
    Article Snippet: Total RNAs from PCa cell lines treated with SINE were extracted and purified by using the RNeasy Mini Kit and RNase-free DNase Set (QIAGEN, Valencia, CA) following the protocol provided by the manufacturer. .. Total RNAs from PCa cell lines treated with SINE were extracted and purified by using the RNeasy Mini Kit and RNase-free DNase Set (QIAGEN, Valencia, CA) following the protocol provided by the manufacturer.

    Article Title: Enhanced Arabidopsis pattern-triggered immunity by overexpression of cysteine-rich receptor-like kinases
    Article Snippet: Paragraph title: RNA extraction and gene expression analysis ... Genomic DNA contaminations were removed using Qiagen RNase-Free DNase Set.

    Article Title: Stem Cells Derived from Lipoma and Adipose Tissue—Similar Mesenchymal Phenotype but Different Differentiation Capacity Governed by Distinct Molecular Signature
    Article Snippet: DNase I RNase-free set (Qiagen) was used for on-column digestion of residual genomic DNA, according to the manufacturer’s instructions. .. Reverse transcription was performed in the PCR thermal cycler SureCycler8800 (Agilent Technologies, Santa Clara, CA, USA).

    Gene Knockout:

    Article Title: Hyperplasia of Interstitial Cells of Cajal in Sprouty Homolog 4 Deficient Mice
    Article Snippet: A minimum of three different RNA samples from P10 WT, Kit K641E/K641E and Spry4 KO mice antrum were used. .. Genomic DNA was removed using the RNase-Free DNase set (Qiagen).

    Flow Cytometry:

    Article Title: Campylobacter coli From Retail Liver and Meat Products Is More Aerotolerant Than Campylobacter jejuni
    Article Snippet: Then, 700 μl of lysate was transferred to RNeasy spin column and centrifuged for 15 s at 13,800 × g. Flow through was discarded and remaining lysate solution was added and centrifuged in the same column. .. After washing and elution steps according to manufacturer's protocol, RNA samples were further subjected to DNA digestion in solution using RNase Free DNase set (Qiagen).

    Infection:

    Article Title: Intrinsic terminators in Mycoplasma hyopneumoniae transcription
    Article Snippet: M. hyopneumoniae strain 7448 isolated from an infected swine (Embrapa, Santa Catarina, Brazil) [ ] was grown in 25 ml of Friis broth [ ] at 37°C for 24 h with gentle agitation in a roller drum. .. The purification was performed according to the manufacturer’s instructions, with on-column DNaseI digestion using the RNase-Free DNase Set (Qiagen, Germany) and a second round of treatment with DNase I (Fermentas, USA).

    Generated:

    Article Title: Analysis of ADP-glucose pyrophosphorylase expression during turion formation induced by abscisic acid in Spirodela polyrhiza (greater duckweed)
    Article Snippet: The on-column DNase I was used to remove contaminating genomic DNA (Qiagen, 79254). .. The on-column DNase I was used to remove contaminating genomic DNA (Qiagen, 79254).

    Article Title: Hyperplasia of Interstitial Cells of Cajal in Sprouty Homolog 4 Deficient Mice
    Article Snippet: Genomic DNA was removed using the RNase-Free DNase set (Qiagen). .. Genomic DNA was removed using the RNase-Free DNase set (Qiagen).

    Article Title: Comparative Transcriptome and iTRAQ Proteome Analyses Reveal the Mechanisms of Diapause in Aphidius gifuensis Ashmead (Hymenoptera: Aphidiidae)
    Article Snippet: Qualified total RNA was further purified with the RNeasy micro kit (Qiagen, Hilden, Germany) and the RNase-Free DNase Set (Qiagen, Hilden, Germany). .. Total RNA was extracted and oligo (dT) magnetic beads and fragmentation buffer were used to generate short fragments of the isolated poly(A) mRNA.

    other:

    Article Title: TLR5 decoy receptor as a novel anti-amyloid therapeutic for Alzheimer’s disease
    Article Snippet: Control samples had slightly lower RINs than AD samples due to limitations in availability of samples in this diagnostic category.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Analysis of ADP-glucose pyrophosphorylase expression during turion formation induced by abscisic acid in Spirodela polyrhiza (greater duckweed)
    Article Snippet: The on-column DNase I was used to remove contaminating genomic DNA (Qiagen, 79254). .. The on-column DNase I was used to remove contaminating genomic DNA (Qiagen, 79254).

    Article Title: Enhanced Arabidopsis pattern-triggered immunity by overexpression of cysteine-rich receptor-like kinases
    Article Snippet: Genomic DNA contaminations were removed using Qiagen RNase-Free DNase Set. .. Genomic DNA contaminations were removed using Qiagen RNase-Free DNase Set.

    Nucleic Acid Electrophoresis:

    Article Title: Intrinsic terminators in Mycoplasma hyopneumoniae transcription
    Article Snippet: The purification was performed according to the manufacturer’s instructions, with on-column DNaseI digestion using the RNase-Free DNase Set (Qiagen, Germany) and a second round of treatment with DNase I (Fermentas, USA). .. DNA absence was monitored to below PCR-detectable levels.

    RNA Sequencing Assay:

    Article Title: Repurposing Ponatinib as a Potent Agent against KIT Mutant Melanomas
    Article Snippet: Paragraph title: RNA-Sequencing and analysis ... Qualified total RNA was further purified by RNAClean XP Kit (Cat A63987, Beckman Coulter,Inc.Kraemer Boulevard Brea, CA,USA)and RNase-Free DNase Set (Cat#79254, QIAGEN, GmBH, Germany).

    Article Title: Laser capture microdissection in Ectocarpus siliculosus: the pathway to cell-specific transcriptomics in brown algae
    Article Snippet: On column DNA digestion was carried out using Qiagen RNase-free DNase I set (#79254). .. On column DNA digestion was carried out using Qiagen RNase-free DNase I set (#79254).

    Fluorescence:

    Article Title: Hyperplasia of Interstitial Cells of Cajal in Sprouty Homolog 4 Deficient Mice
    Article Snippet: Genomic DNA was removed using the RNase-Free DNase set (Qiagen). .. Genomic DNA was removed using the RNase-Free DNase set (Qiagen).

    Isolation:

    Article Title: Definition of the ?W Regulon of Bacillus subtilis in the Absence of Stress
    Article Snippet: Precipitated RNA was washed with 70% ethanol and dissolved in 100 µl of RNase free water. .. The isolated RNA was DNase-treated using the RNase-Free DNase Set (Qiagen) and purified using the RNA Clean-Up and Concentration Micro Kit (Norgen). .. RNA concentrations were measured using a Nanodrop-1000 spectrophotometer and RNA quality was assessed with the Agilent 2100 Bioanalyzer according to the manufacturer's instructions.

    Article Title: Analysis of ADP-glucose pyrophosphorylase expression during turion formation induced by abscisic acid in Spirodela polyrhiza (greater duckweed)
    Article Snippet: Paragraph title: Genomic DNA and total RNA isolation ... The on-column DNase I was used to remove contaminating genomic DNA (Qiagen, 79254).

    Article Title: The Paracrine Feedback Loop Between Vitamin D3 (1,25(OH)2D3) and PTHrP in Prehypertrophic Chondrocytes
    Article Snippet: Paragraph title: RNA isolation and cDNA synthesis ... An additional DNA digestion step with DNase (RNAse-Free DNase Set, 79254, Qiagen) was included to ensure DNA removal.

    Article Title: Intrinsic terminators in Mycoplasma hyopneumoniae transcription
    Article Snippet: Paragraph title: Culture conditions and RNA isolation ... The purification was performed according to the manufacturer’s instructions, with on-column DNaseI digestion using the RNase-Free DNase Set (Qiagen, Germany) and a second round of treatment with DNase I (Fermentas, USA).

    Article Title: The Chromosomal Proteins JIL-1 and Z4/Putzig Regulate the Telomeric Chromatin in Drosophila melanogaster
    Article Snippet: Total RNA was isolated from ten whole third instar larvae and extracted using RNeasy Mini Kit (Qiagen) according to manufacturer's protocol. .. RNase Free DNase Set (Qiagen) was used to remove genomic DNA contaminations as follows: one on column during the extraction accordingly to manufacturer's protocol, and two in solution for 2 hours at 37°C.

    Article Title: Repurposing Ponatinib as a Potent Agent against KIT Mutant Melanomas
    Article Snippet: RNA was isolated from snap-frozen tumors from vehicle-, imatinib-, and ponatinib-treated mice. .. Qualified total RNA was further purified by RNAClean XP Kit (Cat A63987, Beckman Coulter,Inc.Kraemer Boulevard Brea, CA,USA)and RNase-Free DNase Set (Cat#79254, QIAGEN, GmBH, Germany).

    Article Title: Bioinformatics Analyses Determined the Distinct CNS and Peripheral Surrogate Biomarker Candidates Between Two Mouse Models for Progressive Multiple Sclerosis
    Article Snippet: Total RNA was extracted with an RNeasy Mini Kit (Qiagen, Germantown, MD) according to the manufacturer's instructions from brain and spleen homogenate. .. DNase treatment was performed during RNA isolation with an RNase-Free DNase Set (Qiagen). .. All samples were purified to an absorbance ratio (A260/A280) between 1.9 and 2.1 ( ).

    Article Title: Campylobacter coli From Retail Liver and Meat Products Is More Aerotolerant Than Campylobacter jejuni
    Article Snippet: Paragraph title: Isolation of Total RNA ... After washing and elution steps according to manufacturer's protocol, RNA samples were further subjected to DNA digestion in solution using RNase Free DNase set (Qiagen).

    Article Title: Myc-like transcriptional factors in wheat: structural and functional organization of the subfamily I members
    Article Snippet: RNAs from coleoptile samples were extracted using the RNeasy Mini Kit (QIAGEN). .. All isolated RNAs were treated with the RNase-free DNase set (QIAGEN). .. Total RNA was converted to single-stranded cDNA in a 20-μL reaction from a template consisting of 0.5 μg of total RNA using the RevertAid First Strand cDNA Synthesis Kit (Thermo Fisher Scientific Inc.).

    Article Title: Down-regulation of AR splice variants through XPO1 suppression contributes to the inhibition of prostate cancer progression
    Article Snippet: Paragraph title: RNA isolation and mRNA real-time RT-qPCR ... Total RNAs from PCa cell lines treated with SINE were extracted and purified by using the RNeasy Mini Kit and RNase-free DNase Set (QIAGEN, Valencia, CA) following the protocol provided by the manufacturer.

    Article Title: Phenotype and Susceptibility to HIV-infection of CD4+ Th17 Cells in the Human Female Reproductive Tract
    Article Snippet: Paragraph title: RNA isolation and real time PCR ... DNase digestion was done on-column with the RNase-Free DNase set (Qiagen).

    Article Title: Stem Cells Derived from Lipoma and Adipose Tissue—Similar Mesenchymal Phenotype but Different Differentiation Capacity Governed by Distinct Molecular Signature
    Article Snippet: Paragraph title: 2.6. RNA Isolation and Reverse Transcription ... DNase I RNase-free set (Qiagen) was used for on-column digestion of residual genomic DNA, according to the manufacturer’s instructions.

    Polymerase Chain Reaction:

    Article Title: Comparative Transcriptome and iTRAQ Proteome Analyses Reveal the Mechanisms of Diapause in Aphidius gifuensis Ashmead (Hymenoptera: Aphidiidae)
    Article Snippet: Qualified total RNA was further purified with the RNeasy micro kit (Qiagen, Hilden, Germany) and the RNase-Free DNase Set (Qiagen, Hilden, Germany). .. Qualified total RNA was further purified with the RNeasy micro kit (Qiagen, Hilden, Germany) and the RNase-Free DNase Set (Qiagen, Hilden, Germany).

    Article Title: Campylobacter coli From Retail Liver and Meat Products Is More Aerotolerant Than Campylobacter jejuni
    Article Snippet: After washing and elution steps according to manufacturer's protocol, RNA samples were further subjected to DNA digestion in solution using RNase Free DNase set (Qiagen). .. Quantity of total RNA was measured with NanoDrop™ 8,000 spectrophotometer.

    Article Title: Laser capture microdissection in Ectocarpus siliculosus: the pathway to cell-specific transcriptomics in brown algae
    Article Snippet: On column DNA digestion was carried out using Qiagen RNase-free DNase I set (#79254). .. On column DNA digestion was carried out using Qiagen RNase-free DNase I set (#79254).

    Article Title: Enhanced Arabidopsis pattern-triggered immunity by overexpression of cysteine-rich receptor-like kinases
    Article Snippet: Genomic DNA contaminations were removed using Qiagen RNase-Free DNase Set. .. The cDNA was then diluted 5-times before reverse transcription-PCR (RT-PCR) or quantitative RT-PCR (qRT-PCR) analyses.

    Article Title: Stem Cells Derived from Lipoma and Adipose Tissue—Similar Mesenchymal Phenotype but Different Differentiation Capacity Governed by Distinct Molecular Signature
    Article Snippet: DNase I RNase-free set (Qiagen) was used for on-column digestion of residual genomic DNA, according to the manufacturer’s instructions. .. DNase I RNase-free set (Qiagen) was used for on-column digestion of residual genomic DNA, according to the manufacturer’s instructions.

    Microscopy:

    Article Title: Laser capture microdissection in Ectocarpus siliculosus: the pathway to cell-specific transcriptomics in brown algae
    Article Snippet: Laser capture was performed using a Carl Zeiss PALM MicroBeam unit equipped with an AxioVert 200 microscope and a CryLas UV laser, and assisted by the PalmRobo 4.5 software. .. On column DNA digestion was carried out using Qiagen RNase-free DNase I set (#79254).

    Purification:

    Article Title: Definition of the ?W Regulon of Bacillus subtilis in the Absence of Stress
    Article Snippet: Precipitated RNA was washed with 70% ethanol and dissolved in 100 µl of RNase free water. .. The isolated RNA was DNase-treated using the RNase-Free DNase Set (Qiagen) and purified using the RNA Clean-Up and Concentration Micro Kit (Norgen). .. RNA concentrations were measured using a Nanodrop-1000 spectrophotometer and RNA quality was assessed with the Agilent 2100 Bioanalyzer according to the manufacturer's instructions.

    Article Title: Comparative Transcriptome and iTRAQ Proteome Analyses Reveal the Mechanisms of Diapause in Aphidius gifuensis Ashmead (Hymenoptera: Aphidiidae)
    Article Snippet: An Agilent Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA, USA) and the RIN number were used to evaluate RNA integrity. .. Qualified total RNA was further purified with the RNeasy micro kit (Qiagen, Hilden, Germany) and the RNase-Free DNase Set (Qiagen, Hilden, Germany). .. Total RNA was extracted and oligo (dT) magnetic beads and fragmentation buffer were used to generate short fragments of the isolated poly(A) mRNA.

    Article Title: Repurposing Ponatinib as a Potent Agent against KIT Mutant Melanomas
    Article Snippet: Total RNA was extracted using RNeasy Mini Kit (Cat# 74106, Qiagen) following the manufacturer's instructions and checked for a RIN number to inspect RNA integrity by an Agilent Bioanalyzer 2100 (Agilent technologies, Santa Clara, CA, US). .. Qualified total RNA was further purified by RNAClean XP Kit (Cat A63987, Beckman Coulter,Inc.Kraemer Boulevard Brea, CA,USA)and RNase-Free DNase Set (Cat#79254, QIAGEN, GmBH, Germany). .. The Illumina TruSeq RNA Sample Preparation Kit (Illumina) was used for library preparation.

    Article Title: Down-regulation of AR splice variants through XPO1 suppression contributes to the inhibition of prostate cancer progression
    Article Snippet: Anti-AR (N20) which recognizes both AR full length and AR splice variants (Santa Cruz, Santa Cruz, CA), anti-XPO1 (Santa Cruz), anti-FOXA1 (Novus Biologicals, Littleton, CO), anti-MED1 (Santa Cruz), anti-eIF4E (Cell Signaling, Danvers, MA), anti-Src (Cell Signaling), anti-p-Src(Tyr527) (Cell Signaling), anti-Lamin B (Invitrogen), and anti-β-actin (Sigma, St. Louis, MO) primary antibodies were used for Western Blot analysis. .. Total RNAs from PCa cell lines treated with SINE were extracted and purified by using the RNeasy Mini Kit and RNase-free DNase Set (QIAGEN, Valencia, CA) following the protocol provided by the manufacturer. .. Total RNAs from PCa tissues were isolated from formalin-fixed, paraffin-embedded tissue sections by using miRNeasy FFPE Kit (QIAGEN) following the protocol provided by the company.

    Article Title: Laser capture microdissection in Ectocarpus siliculosus: the pathway to cell-specific transcriptomics in brown algae
    Article Snippet: On column DNA digestion was carried out using Qiagen RNase-free DNase I set (#79254). .. On column DNA digestion was carried out using Qiagen RNase-free DNase I set (#79254).

    Article Title: Phenotype and Susceptibility to HIV-infection of CD4+ Th17 Cells in the Human Female Reproductive Tract
    Article Snippet: Total RNA was extracted from purified CD4+ T cells using the micro RNeasy kit (QIAGEN, Valencia, CA). .. DNase digestion was done on-column with the RNase-Free DNase set (Qiagen).

    Article Title: Enhanced Arabidopsis pattern-triggered immunity by overexpression of cysteine-rich receptor-like kinases
    Article Snippet: Total RNA was extracted and purified using the MaestroZol reagent according to the manufacturer's instructions (Omics Biotechnology Co., Ltd) with the addition of PLUS reagent for polysaccharides and proteoglycans elimination. .. Genomic DNA contaminations were removed using Qiagen RNase-Free DNase Set.

    Article Title: Stem Cells Derived from Lipoma and Adipose Tissue—Similar Mesenchymal Phenotype but Different Differentiation Capacity Governed by Distinct Molecular Signature
    Article Snippet: LDSCs and ADSCs at passage 2 (day 0 in differentiation assay), at day 21 in adipogenic differentiation assay and days 8 and 16 in osteogenic differentiation assay were placed in RNAlater® (Ambion, Life Technologies, Carlsbad, CA, USA) and stored at −80°C until RNA purification step. .. DNase I RNase-free set (Qiagen) was used for on-column digestion of residual genomic DNA, according to the manufacturer’s instructions.

    Sequencing:

    Article Title: Hyperplasia of Interstitial Cells of Cajal in Sprouty Homolog 4 Deficient Mice
    Article Snippet: Genomic DNA was removed using the RNase-Free DNase set (Qiagen). .. Genomic DNA was removed using the RNase-Free DNase set (Qiagen).

    Article Title: Comparative Transcriptome and iTRAQ Proteome Analyses Reveal the Mechanisms of Diapause in Aphidius gifuensis Ashmead (Hymenoptera: Aphidiidae)
    Article Snippet: Paragraph title: RNA preparation, cDNA library construction, and illumina sequencing ... Qualified total RNA was further purified with the RNeasy micro kit (Qiagen, Hilden, Germany) and the RNase-Free DNase Set (Qiagen, Hilden, Germany).

    Labeling:

    Article Title: Definition of the ?W Regulon of Bacillus subtilis in the Absence of Stress
    Article Snippet: The isolated RNA was DNase-treated using the RNase-Free DNase Set (Qiagen) and purified using the RNA Clean-Up and Concentration Micro Kit (Norgen). .. RNA concentrations were measured using a Nanodrop-1000 spectrophotometer and RNA quality was assessed with the Agilent 2100 Bioanalyzer according to the manufacturer's instructions.

    Nuclease Assay:

    Article Title: Phenotype and Susceptibility to HIV-infection of CD4+ Th17 Cells in the Human Female Reproductive Tract
    Article Snippet: DNase digestion was done on-column with the RNase-Free DNase set (Qiagen). .. After RNA quantification, cDNA was generated with the iScript cDNA synthesis kit (Bio-Rad, Hercules, CA).

    cDNA Library Assay:

    Article Title: Comparative Transcriptome and iTRAQ Proteome Analyses Reveal the Mechanisms of Diapause in Aphidius gifuensis Ashmead (Hymenoptera: Aphidiidae)
    Article Snippet: Paragraph title: RNA preparation, cDNA library construction, and illumina sequencing ... Qualified total RNA was further purified with the RNeasy micro kit (Qiagen, Hilden, Germany) and the RNase-Free DNase Set (Qiagen, Hilden, Germany).

    Chloramphenicol Acetyltransferase Assay:

    Article Title: Repurposing Ponatinib as a Potent Agent against KIT Mutant Melanomas
    Article Snippet: Total RNA was extracted using RNeasy Mini Kit (Cat# 74106, Qiagen) following the manufacturer's instructions and checked for a RIN number to inspect RNA integrity by an Agilent Bioanalyzer 2100 (Agilent technologies, Santa Clara, CA, US). .. Qualified total RNA was further purified by RNAClean XP Kit (Cat A63987, Beckman Coulter,Inc.Kraemer Boulevard Brea, CA,USA)and RNase-Free DNase Set (Cat79254, QIAGEN, GmBH, Germany). .. The Illumina TruSeq RNA Sample Preparation Kit (Illumina) was used for library preparation.

    Mouse Assay:

    Article Title: Hyperplasia of Interstitial Cells of Cajal in Sprouty Homolog 4 Deficient Mice
    Article Snippet: A minimum of three different RNA samples from P10 WT, Kit K641E/K641E and Spry4 KO mice antrum were used. .. Genomic DNA was removed using the RNase-Free DNase set (Qiagen).

    Article Title: Repurposing Ponatinib as a Potent Agent against KIT Mutant Melanomas
    Article Snippet: RNA was isolated from snap-frozen tumors from vehicle-, imatinib-, and ponatinib-treated mice. .. Qualified total RNA was further purified by RNAClean XP Kit (Cat A63987, Beckman Coulter,Inc.Kraemer Boulevard Brea, CA,USA)and RNase-Free DNase Set (Cat#79254, QIAGEN, GmBH, Germany).

    Article Title: Bioinformatics Analyses Determined the Distinct CNS and Peripheral Surrogate Biomarker Candidates Between Two Mouse Models for Progressive Multiple Sclerosis
    Article Snippet: Brains and spleens from three to six mice per group were homogenized individually in TRI-Reagent® (Molecular Research Center, Cincinnati, OH), using the Kinematica Polytron™ homogenizer (Kinematica, Bohemia, NY). .. DNase treatment was performed during RNA isolation with an RNase-Free DNase Set (Qiagen).

    Software:

    Article Title: Laser capture microdissection in Ectocarpus siliculosus: the pathway to cell-specific transcriptomics in brown algae
    Article Snippet: Laser capture was performed using a Carl Zeiss PALM MicroBeam unit equipped with an AxioVert 200 microscope and a CryLas UV laser, and assisted by the PalmRobo 4.5 software. .. On column DNA digestion was carried out using Qiagen RNase-free DNase I set (#79254).

    Real-time Polymerase Chain Reaction:

    Article Title: Hyperplasia of Interstitial Cells of Cajal in Sprouty Homolog 4 Deficient Mice
    Article Snippet: Paragraph title: Real time quantitative PCR (qPCR) ... Genomic DNA was removed using the RNase-Free DNase set (Qiagen).

    Article Title: Effects of Obesity and Metabolic Syndrome on Steroidogenesis and Folliculogenesis in the Female Ossabaw Mini-Pig
    Article Snippet: Paragraph title: Quantitative real time PCR ... Total RNA was extracted from cell aliquots of 100,000 cells using the RNeasy Mini Kit (Qiagen Inc) and an on-column DNase treatment (RNase-free DNase Set, Qiagen Inc).

    Article Title: The Chromosomal Proteins JIL-1 and Z4/Putzig Regulate the Telomeric Chromatin in Drosophila melanogaster
    Article Snippet: RNase Free DNase Set (Qiagen) was used to remove genomic DNA contaminations as follows: one on column during the extraction accordingly to manufacturer's protocol, and two in solution for 2 hours at 37°C. .. RNase Free DNase Set (Qiagen) was used to remove genomic DNA contaminations as follows: one on column during the extraction accordingly to manufacturer's protocol, and two in solution for 2 hours at 37°C.

    Article Title: Down-regulation of AR splice variants through XPO1 suppression contributes to the inhibition of prostate cancer progression
    Article Snippet: Total RNAs from PCa cell lines treated with SINE were extracted and purified by using the RNeasy Mini Kit and RNase-free DNase Set (QIAGEN, Valencia, CA) following the protocol provided by the manufacturer. .. The expression levels of AR, AR-v7, ARv567es, PSA, XPO1, FOXA1, MED1, Src, Vav3, Sam68, or UBE2C in selinexor or KPT-8602 treated or un-treated PCa cells and PCa tissues were analyzed by real-time RT-qPCR using High Capacity cDNA Reverse Transcription Kit and SYBR Green Master Mixture from Applied Biosystems (Waltham, MA).

    Article Title: Laser capture microdissection in Ectocarpus siliculosus: the pathway to cell-specific transcriptomics in brown algae
    Article Snippet: On column DNA digestion was carried out using Qiagen RNase-free DNase I set (#79254). .. On column DNA digestion was carried out using Qiagen RNase-free DNase I set (#79254).

    Article Title: Phenotype and Susceptibility to HIV-infection of CD4+ Th17 Cells in the Human Female Reproductive Tract
    Article Snippet: Paragraph title: RNA isolation and real time PCR ... DNase digestion was done on-column with the RNase-Free DNase set (Qiagen).

    Article Title: Stem Cells Derived from Lipoma and Adipose Tissue—Similar Mesenchymal Phenotype but Different Differentiation Capacity Governed by Distinct Molecular Signature
    Article Snippet: DNase I RNase-free set (Qiagen) was used for on-column digestion of residual genomic DNA, according to the manufacturer’s instructions. .. Reverse transcription was performed in the PCR thermal cycler SureCycler8800 (Agilent Technologies, Santa Clara, CA, USA).

    RNA Extraction:

    Article Title: Duplicated Leptin Receptors in Two Species of Eel Bring New Insights into the Evolution of the Leptin System in Vertebrates
    Article Snippet: Paragraph title: RNA extraction ... A deoxyribonuclease I treatment, using the Qiagen RNase-free DNase Set, was applied during the procedure, according to the manufacturer instructions.

    Article Title: Comparative Transcriptome and iTRAQ Proteome Analyses Reveal the Mechanisms of Diapause in Aphidius gifuensis Ashmead (Hymenoptera: Aphidiidae)
    Article Snippet: Total RNA was extracted using RNAiso Plus Total RNA extraction reagent (TaKaRa Bio Inc., Kusatsu, Shiga, Japan) following the manufacturer's instructions. .. Qualified total RNA was further purified with the RNeasy micro kit (Qiagen, Hilden, Germany) and the RNase-Free DNase Set (Qiagen, Hilden, Germany).

    Article Title: The Chromosomal Proteins JIL-1 and Z4/Putzig Regulate the Telomeric Chromatin in Drosophila melanogaster
    Article Snippet: Paragraph title: RNA extraction and cDNA synthesis ... RNase Free DNase Set (Qiagen) was used to remove genomic DNA contaminations as follows: one on column during the extraction accordingly to manufacturer's protocol, and two in solution for 2 hours at 37°C.

    Article Title: Laser capture microdissection in Ectocarpus siliculosus: the pathway to cell-specific transcriptomics in brown algae
    Article Snippet: The PicoPure RNA extraction kit from Life Technologies (#KIT0204) was used to extract RNA. .. On column DNA digestion was carried out using Qiagen RNase-free DNase I set (#79254).

    Article Title: Enhanced Arabidopsis pattern-triggered immunity by overexpression of cysteine-rich receptor-like kinases
    Article Snippet: Paragraph title: RNA extraction and gene expression analysis ... Genomic DNA contaminations were removed using Qiagen RNase-Free DNase Set.

    Homogenization:

    Article Title: Duplicated Leptin Receptors in Two Species of Eel Bring New Insights into the Evolution of the Leptin System in Vertebrates
    Article Snippet: Extraction of total RNA was performed using mechanical homogenization (TissueLyser II, Qiagen, Hilden, Germany). .. A deoxyribonuclease I treatment, using the Qiagen RNase-free DNase Set, was applied during the procedure, according to the manufacturer instructions.

    Incubation:

    Article Title: Campylobacter coli From Retail Liver and Meat Products Is More Aerotolerant Than Campylobacter jejuni
    Article Snippet: For total RNA isolation, 2 ml RNA protect bacteria reagent (Qiagen) was added to 1 ml of bacterial culture and vortexed for 5 s followed by incubation at room temperature for 5 min. Bacterial cell was harvested by centrifugation at 5,000 × g for 5 min at 4°C and cell pellet was resuspended in 700 μl of lysis buffer (RLT buffer, Qiagen) by vortexing for 10 s. Bacterial cell lysis was done by vortexing vigorously for 5 min in 2 ml safe lock tube containing ~30 mg acid washed glass beads (212–300 μm, Sigma, G1277). .. After washing and elution steps according to manufacturer's protocol, RNA samples were further subjected to DNA digestion in solution using RNase Free DNase set (Qiagen).

    Spectrophotometry:

    Article Title: Campylobacter coli From Retail Liver and Meat Products Is More Aerotolerant Than Campylobacter jejuni
    Article Snippet: After washing and elution steps according to manufacturer's protocol, RNA samples were further subjected to DNA digestion in solution using RNase Free DNase set (Qiagen). .. After washing and elution steps according to manufacturer's protocol, RNA samples were further subjected to DNA digestion in solution using RNase Free DNase set (Qiagen).

    Article Title: Laser capture microdissection in Ectocarpus siliculosus: the pathway to cell-specific transcriptomics in brown algae
    Article Snippet: On column DNA digestion was carried out using Qiagen RNase-free DNase I set (#79254). .. On column DNA digestion was carried out using Qiagen RNase-free DNase I set (#79254).

    Concentration Assay:

    Article Title: Definition of the ?W Regulon of Bacillus subtilis in the Absence of Stress
    Article Snippet: Precipitated RNA was washed with 70% ethanol and dissolved in 100 µl of RNase free water. .. The isolated RNA was DNase-treated using the RNase-Free DNase Set (Qiagen) and purified using the RNA Clean-Up and Concentration Micro Kit (Norgen). .. RNA concentrations were measured using a Nanodrop-1000 spectrophotometer and RNA quality was assessed with the Agilent 2100 Bioanalyzer according to the manufacturer's instructions.

    Differentiation Assay:

    Article Title: Stem Cells Derived from Lipoma and Adipose Tissue—Similar Mesenchymal Phenotype but Different Differentiation Capacity Governed by Distinct Molecular Signature
    Article Snippet: LDSCs and ADSCs at passage 2 (day 0 in differentiation assay), at day 21 in adipogenic differentiation assay and days 8 and 16 in osteogenic differentiation assay were placed in RNAlater® (Ambion, Life Technologies, Carlsbad, CA, USA) and stored at −80°C until RNA purification step. .. DNase I RNase-free set (Qiagen) was used for on-column digestion of residual genomic DNA, according to the manufacturer’s instructions.

    Lysis:

    Article Title: Definition of the ?W Regulon of Bacillus subtilis in the Absence of Stress
    Article Snippet: The frozen powder was resuspended in 4 mL prewarmed (50°C) lysis solution (4 M guanidine thiocyanate, 25 mM sodium acetate [pH 5.2], 0.5% N -laurylsarcosinate [wt/vol]) and immediately frozen in liquid nitrogen. .. The isolated RNA was DNase-treated using the RNase-Free DNase Set (Qiagen) and purified using the RNA Clean-Up and Concentration Micro Kit (Norgen).

    Article Title: Intrinsic terminators in Mycoplasma hyopneumoniae transcription
    Article Snippet: For cell lyses, 0.7 ml of RNeasy Lysis Buffer (RLT buffer) in the presence of 0.134 M of β-mercaptoethanol was used per cultivation flask. .. The purification was performed according to the manufacturer’s instructions, with on-column DNaseI digestion using the RNase-Free DNase Set (Qiagen, Germany) and a second round of treatment with DNase I (Fermentas, USA).

    Article Title: Campylobacter coli From Retail Liver and Meat Products Is More Aerotolerant Than Campylobacter jejuni
    Article Snippet: For total RNA isolation, 2 ml RNA protect bacteria reagent (Qiagen) was added to 1 ml of bacterial culture and vortexed for 5 s followed by incubation at room temperature for 5 min. Bacterial cell was harvested by centrifugation at 5,000 × g for 5 min at 4°C and cell pellet was resuspended in 700 μl of lysis buffer (RLT buffer, Qiagen) by vortexing for 10 s. Bacterial cell lysis was done by vortexing vigorously for 5 min in 2 ml safe lock tube containing ~30 mg acid washed glass beads (212–300 μm, Sigma, G1277). .. After washing and elution steps according to manufacturer's protocol, RNA samples were further subjected to DNA digestion in solution using RNase Free DNase set (Qiagen).

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  • 99
    Qiagen rnase free dnase
    Enzymatic digestion of decellularized ECM scaffolds releases small RNA molecules. ( A ) Nucleic acid extracted from untreated UBM (no digest) and pepsin-, proteinase K–, or collagenase-treated UBM was exposed to <t>RNase</t> A, <t>DNase</t> I, or no-nuclease treatment (control). ( B ) Electropherogram depicting the small RNA pattern of nucleic acid in fluorescence units (FU) before (top panel) and after (bottom panel) DNase I treatment. ( C ) Electropherogram depicting small RNA pattern from the indicated samples in FU. ( D ) A subset of nucleic molecules in biologic scaffolds is protected from nuclease degradation.
    Rnase Free Dnase, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 355 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rnase free dnase/product/Qiagen
    Average 99 stars, based on 355 article reviews
    Price from $9.99 to $1999.99
    rnase free dnase - by Bioz Stars, 2019-12
    99/100 stars
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    99
    Qiagen ie mater transgenic mice
    Effect of NTx with regard to development of anti-MATER and ANA in wild-type and IE-Mater <t>transgenic</t> mice. A, Anti-MATER antibodies: Based on immunoblotting assay, 77% of the thymectomized wild-type (n = 17) and 24% of the thymectomized IE-Mater transgenic
    Ie Mater Transgenic Mice, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ie mater transgenic mice/product/Qiagen
    Average 99 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    ie mater transgenic mice - by Bioz Stars, 2019-12
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    Image Search Results


    Enzymatic digestion of decellularized ECM scaffolds releases small RNA molecules. ( A ) Nucleic acid extracted from untreated UBM (no digest) and pepsin-, proteinase K–, or collagenase-treated UBM was exposed to RNase A, DNase I, or no-nuclease treatment (control). ( B ) Electropherogram depicting the small RNA pattern of nucleic acid in fluorescence units (FU) before (top panel) and after (bottom panel) DNase I treatment. ( C ) Electropherogram depicting small RNA pattern from the indicated samples in FU. ( D ) A subset of nucleic molecules in biologic scaffolds is protected from nuclease degradation.

    Journal: Science Advances

    Article Title: Matrix-bound nanovesicles within ECM bioscaffolds

    doi: 10.1126/sciadv.1600502

    Figure Lengend Snippet: Enzymatic digestion of decellularized ECM scaffolds releases small RNA molecules. ( A ) Nucleic acid extracted from untreated UBM (no digest) and pepsin-, proteinase K–, or collagenase-treated UBM was exposed to RNase A, DNase I, or no-nuclease treatment (control). ( B ) Electropherogram depicting the small RNA pattern of nucleic acid in fluorescence units (FU) before (top panel) and after (bottom panel) DNase I treatment. ( C ) Electropherogram depicting small RNA pattern from the indicated samples in FU. ( D ) A subset of nucleic molecules in biologic scaffolds is protected from nuclease degradation.

    Article Snippet: RNase-free DNase was obtained from Qiagen.

    Techniques: Fluorescence

    Extracellular vesicle internalization and transfer of GFP mRNA ( A ) Super resolution 3D reconstruction of GFP+ mRPC following 24 h incubation with PKH26 labeled extracellular vesicles. Red vesicles are visibly localized near the cell surface and within cytoplasm. In the XZ axis, GFP (green), EVs (red) and nuclei (blue, DAPI). ( B ) same as ( A ) with GFP (FITC) channel removed to increase visibility of PKH26 (TRITC) labeled EVs. Each panel contains three cross-sectional views (xy, xz, and yz). Scale: 5 µm. ( C ) RT-PCR analysis of GFP mRNA transfer between GFP+ mRPCs and non-GFP hRPCs. Non-GFP hRPCs served as negative control; GFP+ mRPCs served as postive control. GAPDH served as the internal control gene. EVs were treated using an RNase-Free DNase Set to remove DNA comtamination before cDNA synthesis. ( D ) Intensities of RT-PCR images were measured with ImageJ software and normalized to GAPDH. Relative levels of hRPC GFP after transfer of EVs is significantly higher than negative control.

    Journal: Scientific Reports

    Article Title: Retinal progenitor cells release extracellular vesicles containing developmental transcription factors, microRNA and membrane proteins

    doi: 10.1038/s41598-018-20421-1

    Figure Lengend Snippet: Extracellular vesicle internalization and transfer of GFP mRNA ( A ) Super resolution 3D reconstruction of GFP+ mRPC following 24 h incubation with PKH26 labeled extracellular vesicles. Red vesicles are visibly localized near the cell surface and within cytoplasm. In the XZ axis, GFP (green), EVs (red) and nuclei (blue, DAPI). ( B ) same as ( A ) with GFP (FITC) channel removed to increase visibility of PKH26 (TRITC) labeled EVs. Each panel contains three cross-sectional views (xy, xz, and yz). Scale: 5 µm. ( C ) RT-PCR analysis of GFP mRNA transfer between GFP+ mRPCs and non-GFP hRPCs. Non-GFP hRPCs served as negative control; GFP+ mRPCs served as postive control. GAPDH served as the internal control gene. EVs were treated using an RNase-Free DNase Set to remove DNA comtamination before cDNA synthesis. ( D ) Intensities of RT-PCR images were measured with ImageJ software and normalized to GAPDH. Relative levels of hRPC GFP after transfer of EVs is significantly higher than negative control.

    Article Snippet: DNA contamination was removed using an RNase-Free DNase Set (Qiagen). cDNA was synthesized using equal amounts of RNA samples (800 ng), according to the AMV First Strand cDNA Synthesis Kit instructions (NEB Biolabs). β-actin and GAPDH were used as a housekeeping gene controls for mRNA analysis , .

    Techniques: Incubation, Labeling, Reverse Transcription Polymerase Chain Reaction, Negative Control, Software

    Aar modulates DNA-binding properties of H-NS. orf2223  and  orf3928  probes (Panel A) were incubated with different concentration of H-NS protein and analyzed by EMSA (Panel B). H-NS binding sites are indicated in red. In a second experiment, the samples were incubated with either H-NS, Aar or both proteins and analyzed by EMSA (Panel C). In parallel,  proV  was incubated with H-NS in either presence or absence of Aar. The presence of Aar abolished the H-NS-DNA interaction (panel D, white arrows, lanes 2, 3, 5, 6). A similar approach was used to evaluate the effect of using increasing concentrations of Aar (0.8–12.8 μM) (panel E, lanes 3 to 7). DNA probe and H-NS samples were incubated in either presence or absence of Aar and treated with DNase I. Incubation of samples with Aar (3.2 μM) disrupted DNA protection and increased DNase I effect on  proV  probe (panel F, black arrows, lanes 6, 7, 13, 14).

    Journal: PLoS Pathogens

    Article Title: The AraC Negative Regulator family modulates the activity of histone-like proteins in pathogenic bacteria

    doi: 10.1371/journal.ppat.1006545

    Figure Lengend Snippet: Aar modulates DNA-binding properties of H-NS. orf2223 and orf3928 probes (Panel A) were incubated with different concentration of H-NS protein and analyzed by EMSA (Panel B). H-NS binding sites are indicated in red. In a second experiment, the samples were incubated with either H-NS, Aar or both proteins and analyzed by EMSA (Panel C). In parallel, proV was incubated with H-NS in either presence or absence of Aar. The presence of Aar abolished the H-NS-DNA interaction (panel D, white arrows, lanes 2, 3, 5, 6). A similar approach was used to evaluate the effect of using increasing concentrations of Aar (0.8–12.8 μM) (panel E, lanes 3 to 7). DNA probe and H-NS samples were incubated in either presence or absence of Aar and treated with DNase I. Incubation of samples with Aar (3.2 μM) disrupted DNA protection and increased DNase I effect on proV probe (panel F, black arrows, lanes 6, 7, 13, 14).

    Article Snippet: For the DNase I protection assays, after protein-DNA complex formation, the samples were incubated with 25 ηg of DNase I (RNase-free DNase set) (Qiagen) for 30 min.

    Techniques: Binding Assay, Incubation, Concentration Assay

    Effect of NTx with regard to development of anti-MATER and ANA in wild-type and IE-Mater transgenic mice. A, Anti-MATER antibodies: Based on immunoblotting assay, 77% of the thymectomized wild-type (n = 17) and 24% of the thymectomized IE-Mater transgenic

    Journal:

    Article Title: Autoimmune Oophoritis with Multiple Molecular Targets Mitigated by Transgenic Expression of Mater

    doi: 10.1210/en.2011-0022

    Figure Lengend Snippet: Effect of NTx with regard to development of anti-MATER and ANA in wild-type and IE-Mater transgenic mice. A, Anti-MATER antibodies: Based on immunoblotting assay, 77% of the thymectomized wild-type (n = 17) and 24% of the thymectomized IE-Mater transgenic

    Article Snippet: RNA was extracted from thymus, spleen, and ovaries of wild-type (C57BL/6J) and IE-Mater transgenic mice (QIAGEN RNeasy Mini Kit with RNase-Free DNase Set) followed by reverse transcription (RT) (QIAGEN Omniscript RT Kit) of the purified mRNA with oligo dT18 primers.

    Techniques: Transgenic Assay, Mouse Assay

    Incidence of autoimmune oophoritis after NTx in wild-type and IE-Mater transgenic mice. A, Histopathology of autoimmune oophoritis after NTx. Representative sections of ovarian histology with hematoxylin and eosin staining are shown for sham-operated

    Journal:

    Article Title: Autoimmune Oophoritis with Multiple Molecular Targets Mitigated by Transgenic Expression of Mater

    doi: 10.1210/en.2011-0022

    Figure Lengend Snippet: Incidence of autoimmune oophoritis after NTx in wild-type and IE-Mater transgenic mice. A, Histopathology of autoimmune oophoritis after NTx. Representative sections of ovarian histology with hematoxylin and eosin staining are shown for sham-operated

    Article Snippet: RNA was extracted from thymus, spleen, and ovaries of wild-type (C57BL/6J) and IE-Mater transgenic mice (QIAGEN RNeasy Mini Kit with RNase-Free DNase Set) followed by reverse transcription (RT) (QIAGEN Omniscript RT Kit) of the purified mRNA with oligo dT18 primers.

    Techniques: Transgenic Assay, Mouse Assay, Histopathology, Staining

    Antioocyte autoantibodies in wild-type and IE-Mater transgenic mice. A, Incidence. Based on immunofluorescence on frozen ovarian sections (B) and confirmation with the immunoblotting assay, the sera from 82% of the wild-type (n = 17) and 28% of the IE-Mater

    Journal:

    Article Title: Autoimmune Oophoritis with Multiple Molecular Targets Mitigated by Transgenic Expression of Mater

    doi: 10.1210/en.2011-0022

    Figure Lengend Snippet: Antioocyte autoantibodies in wild-type and IE-Mater transgenic mice. A, Incidence. Based on immunofluorescence on frozen ovarian sections (B) and confirmation with the immunoblotting assay, the sera from 82% of the wild-type (n = 17) and 28% of the IE-Mater

    Article Snippet: RNA was extracted from thymus, spleen, and ovaries of wild-type (C57BL/6J) and IE-Mater transgenic mice (QIAGEN RNeasy Mini Kit with RNase-Free DNase Set) followed by reverse transcription (RT) (QIAGEN Omniscript RT Kit) of the purified mRNA with oligo dT18 primers.

    Techniques: Transgenic Assay, Mouse Assay, Immunofluorescence