rnase free dnase i  (Roche)


Bioz Verified Symbol Roche is a verified supplier
Bioz Manufacturer Symbol Roche manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    Roche rnase free dnase i
    Rnase Free Dnase I, supplied by Roche, used in various techniques. Bioz Stars score: 94/100, based on 358 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rnase free dnase i/product/Roche
    Average 94 stars, based on 358 article reviews
    Price from $9.99 to $1999.99
    rnase free dnase i - by Bioz Stars, 2020-09
    94/100 stars

    Images

    Related Articles

    Incubation:

    Article Title: Regulation of ppk Expression and In Vivo Function of Ppk in Streptomyces lividans TK24
    Article Snippet: .. A sample of the extracted products, corresponding to 20 μg of protein, was incubated for 4 h at 37°C in the presence of 3 U/μl RNase-free DNase I from Boehringer, 1.25 μg/μl RNase A from Roche, 0.25 μg/μl pronase from Sigma, and 2.5 U/μl CIPA from Roche. ..

    Purification:

    Article Title: Diabetes-induced lncRNA Dnm3os regulates macrophage functions and inflammation via nuclear mechanisms
    Article Snippet: .. Biotinylated Dnm3o s sense and antisense RNAs were treated with RNase-free DNase I and purified on G-50 Sephadex Quick Spin columns (Cat No.11274015001, Roche). .. Biotinylation efficiency for sense and anti-sense strands were determined by Biotin Chromogenic detection Kit (Cat No. K0661, Thermo Scientific).

    Article Title: Functional Demarcation of Active and Silent Chromatin Domains in Human HOX Loci by Non-Coding RNAs
    Article Snippet: .. Biotinylated RNAs were treated with RNase-free DNase I and purified on G-50 Sephadex Quick Spin columns (Roche). .. 10 pmol biotinylated RNA was heated to 60 C for 10 min and slow cooled to 4 C. RNA was mixed with 100 μg of pre-cleared transcription and splicing-competent HeLa nuclear extract (Gozani et al., 1994) in RIP buffer supplemented with tRNA (0.1 μg/ul) and incubated at 4 C for 1 hour.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 85
    Roche ribonuclease free deoxyribonuclease dnase i
    Identification and functional analysis of an evolutionarily conserved GRE/MRE located at −5.3 kb in the mouse (−4.6 kb in the human) Klf9 gene. A, <t>DNAse</t> I protection assay with the hGR-DBD of the evolutionary conserved 179-bp fragment
    Ribonuclease Free Deoxyribonuclease Dnase I, supplied by Roche, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ribonuclease free deoxyribonuclease dnase i/product/Roche
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ribonuclease free deoxyribonuclease dnase i - by Bioz Stars, 2020-09
    85/100 stars
      Buy from Supplier

    94
    Roche dnase i
    DNase I-PCR Assay of the <t>DNase</t> I Sensitivity of a Transgenic Enhancer Sequence. (A) . (B) A diagram illustrating the design of a PCR primer that allows the transgenic L3 locus, but not the endogenous L3, to be specifically amplified. (C) DNase I-PCR shows differential DNase I sensitivity of chromatin in selected genomic regions. The DNase I sensitivity of the transgenic L3 locus was similar to that of the open chromatin control ACTIN7 .
    Dnase I, supplied by Roche, used in various techniques. Bioz Stars score: 94/100, based on 650 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dnase i/product/Roche
    Average 94 stars, based on 650 article reviews
    Price from $9.99 to $1999.99
    dnase i - by Bioz Stars, 2020-09
    94/100 stars
      Buy from Supplier

    Image Search Results


    Identification and functional analysis of an evolutionarily conserved GRE/MRE located at −5.3 kb in the mouse (−4.6 kb in the human) Klf9 gene. A, DNAse I protection assay with the hGR-DBD of the evolutionary conserved 179-bp fragment

    Journal: Endocrinology

    Article Title: Molecular Basis for Glucocorticoid Induction of the Kr?ppel-Like Factor 9 Gene in Hippocampal Neurons

    doi: 10.1210/en.2012-1303

    Figure Lengend Snippet: Identification and functional analysis of an evolutionarily conserved GRE/MRE located at −5.3 kb in the mouse (−4.6 kb in the human) Klf9 gene. A, DNAse I protection assay with the hGR-DBD of the evolutionary conserved 179-bp fragment

    Article Snippet: We treated 1 μg of total RNA with 20 U of ribonuclease-free deoxyribonuclease (DNAse) I (Roche, Indianapolis, IN) ( ) before cDNA synthesis with the High Capacity Reverse Transcription kit (Invitrogen) with or without the addition of RT.

    Techniques: Functional Assay

    DNase I-PCR Assay of the DNase I Sensitivity of a Transgenic Enhancer Sequence. (A) . (B) A diagram illustrating the design of a PCR primer that allows the transgenic L3 locus, but not the endogenous L3, to be specifically amplified. (C) DNase I-PCR shows differential DNase I sensitivity of chromatin in selected genomic regions. The DNase I sensitivity of the transgenic L3 locus was similar to that of the open chromatin control ACTIN7 .

    Journal: The Plant Cell

    Article Title: Genome-Wide Prediction and Validation of Intergenic Enhancers in Arabidopsis Using Open Chromatin Signatures [OPEN]

    doi: 10.1105/tpc.15.00537

    Figure Lengend Snippet: DNase I-PCR Assay of the DNase I Sensitivity of a Transgenic Enhancer Sequence. (A) . (B) A diagram illustrating the design of a PCR primer that allows the transgenic L3 locus, but not the endogenous L3, to be specifically amplified. (C) DNase I-PCR shows differential DNase I sensitivity of chromatin in selected genomic regions. The DNase I sensitivity of the transgenic L3 locus was similar to that of the open chromatin control ACTIN7 .

    Article Snippet: A DNase I (RNase-free; 10 U/μL; Roche Applied Science; catalog number 04716728001) dilution series was prepared by step-wise dilution using digestion buffer.

    Techniques: Polymerase Chain Reaction, Transgenic Assay, Sequencing, Amplification

    DNase I footprinting analysis of the −204/+70 region of the α3 promoter

    Journal:

    Article Title: The expression of the human neuronal ?3 Na+,K+-ATPase subunit gene is regulated by the activity of the Sp1 and NF-Y transcription factors

    doi: 10.1042/BJ20041294

    Figure Lengend Snippet: DNase I footprinting analysis of the −204/+70 region of the α3 promoter

    Article Snippet: DNase I (DNase I RNase-free; Hoffmann-La Roche, Basel, Switzerland) was diluted in 1× binding buffer, 100 mM KCl and 20 mM MgCl2 , and used at concentrations of 0.05–0.2 units/μg of DNA without extract and 1.5 units/μg of DNA in the presence of extract.

    Techniques: Footprinting

    Electrophoretic analysis of PlcR species. Lysates of B. anthracis SdT2 and SdT12 were probed with C-PlcR antiserum 1451. (A) Lysates made in the presence of 10 mM EDTA. (B) Lysates made in the presence of 10 mM magnesium acetate and then treated with DNase I for 30 min at 37°C. All the samples were made to contain 2% SDS and were heated for 5 min at 95°C before electrophoresis. Putative identities shown in the right margin were determined only on the basis of molecular mass. M, molecular mass markers.

    Journal: Infection and Immunity

    Article Title: A Spontaneous Translational Fusion of Bacillus cereus PlcR and PapR Activates Transcription of PlcR-Dependent Genes in Bacillus anthracis via Binding with a Specific Palindromic Sequence

    doi: 10.1128/IAI.72.10.5814-5823.2004

    Figure Lengend Snippet: Electrophoretic analysis of PlcR species. Lysates of B. anthracis SdT2 and SdT12 were probed with C-PlcR antiserum 1451. (A) Lysates made in the presence of 10 mM EDTA. (B) Lysates made in the presence of 10 mM magnesium acetate and then treated with DNase I for 30 min at 37°C. All the samples were made to contain 2% SDS and were heated for 5 min at 95°C before electrophoresis. Putative identities shown in the right margin were determined only on the basis of molecular mass. M, molecular mass markers.

    Article Snippet: The lysates from the second group were treated with DNase I (RNase free; Roche Diagnostics GmbH, Mannheim, Germany) for 30 min at 37°C (1 U of the DNase per μl of lysate) and then used for Western immunoblotting experiments.

    Techniques: Electrophoresis

    Stability of PlcR complexes. Whole-cell proteins from 12-h cultures of B. anthracis SdT2 and SdT12 were treated sequentially with DNase I and Triton X-100 or with DNase I and urea. Triton-containing samples were heated in SDS sample buffer, whereas urea-containing samples were loaded directly onto the gels. C-PlcR antiserum 1451 was used for the blotting analysis. M, molecular mass markers.

    Journal: Infection and Immunity

    Article Title: A Spontaneous Translational Fusion of Bacillus cereus PlcR and PapR Activates Transcription of PlcR-Dependent Genes in Bacillus anthracis via Binding with a Specific Palindromic Sequence

    doi: 10.1128/IAI.72.10.5814-5823.2004

    Figure Lengend Snippet: Stability of PlcR complexes. Whole-cell proteins from 12-h cultures of B. anthracis SdT2 and SdT12 were treated sequentially with DNase I and Triton X-100 or with DNase I and urea. Triton-containing samples were heated in SDS sample buffer, whereas urea-containing samples were loaded directly onto the gels. C-PlcR antiserum 1451 was used for the blotting analysis. M, molecular mass markers.

    Article Snippet: The lysates from the second group were treated with DNase I (RNase free; Roche Diagnostics GmbH, Mannheim, Germany) for 30 min at 37°C (1 U of the DNase per μl of lysate) and then used for Western immunoblotting experiments.

    Techniques: