rnase a stock  (Qiagen)

 
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    Name:
    QIAGEN Large Construct Kit
    Description:
    For purification of up to 50 μg BAC PAC and P1 DNA or up to 200 μg cosmid DNA free of genomic DNA Kit contents Qiagen Large Construct Kit 500mL Culture Volume BAC PAC P1 cosmid DNA Plasmid Type Anion exchange Technology Manual Processing 280 min Time Run 1 Sample Run 150g Yield For Purification of up to 50μg BAC PAC and P1 DNA or up to 200μg Cosmid DNA Free of genomic DNA Ideal for Subcloning Transfection Sequencing Optimized Gravity flow Procedure Includes 10 Qiagen tip 500 Reagents Buffers ATP Dependent Exonuclease Benefits Efficient removal of genomic DNA contamination Pure transfection grade large construct DNA Easy and simple proced
    Catalog Number:
    12462
    Price:
    433
    Category:
    QIAGEN Large Construct Kit
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    Structured Review

    Qiagen rnase a stock
    QIAGEN Large Construct Kit
    For purification of up to 50 μg BAC PAC and P1 DNA or up to 200 μg cosmid DNA free of genomic DNA Kit contents Qiagen Large Construct Kit 500mL Culture Volume BAC PAC P1 cosmid DNA Plasmid Type Anion exchange Technology Manual Processing 280 min Time Run 1 Sample Run 150g Yield For Purification of up to 50μg BAC PAC and P1 DNA or up to 200μg Cosmid DNA Free of genomic DNA Ideal for Subcloning Transfection Sequencing Optimized Gravity flow Procedure Includes 10 Qiagen tip 500 Reagents Buffers ATP Dependent Exonuclease Benefits Efficient removal of genomic DNA contamination Pure transfection grade large construct DNA Easy and simple proced
    https://www.bioz.com/result/rnase a stock/product/Qiagen
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rnase a stock - by Bioz Stars, 2020-08
    99/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Identification of Genes and Pathways Related to Phenol Degradation in Metagenomic Libraries from Petroleum Refinery Wastewater
    Article Snippet: .. After growth, these clones were pooled into a final volume of approximately 500 mL, which was used for fosmid extraction with the Large-Construction Kit (Qiagen, USA), according to the manufacturer's protocol. .. Finally, five µg of pooled fosmid DNA were used for the pyrosequencing technique, which was done according to the 454/Roche GS-FLX instruction protocols.

    Transfection:

    Article Title: Tissue-specific and neural activity-regulated expression of human BDNF gene in BAC transgenic mice
    Article Snippet: .. In order to validate the reporter activity, BAC DNA was purified using the Large Construct Purification Kit (Qiagen, USA) and transfected into COS-7 cells using DEAE-dextran mediated transfection system [ ]. .. Five days after transfection EGFP expression and distribution in COS-7 cells was visualized using fluorescence microscopy (Eclipse 80i upright microscope, Nikon). hEGFP-BDNF BAC DNA was purified for microinjection by alkaline lysis and linearized with PI-SceI enzyme (NEB, USA).

    BAC Assay:

    Article Title: Contiguous Genomic DNA Sequence Comprising the 19-kD Zein Gene Family from Maize 1
    Article Snippet: .. Large-scale BAC DNA preparations were conducted by using a Large Construction Kit (Qiagen, Inc., Valencia, CA). .. BAC DNAs were physically sheared using the HydroShear instrument (Genomic Instrumentation Services, Inc., San Carlos, CA) with varying speed codes.

    Article Title: Alcelaphine herpesvirus 1 genes A7 and A8 regulate viral spread and are essential for malignant catarrhal fever
    Article Snippet: .. BAC clone DNA was purified using the Large Construct DNA prep kit (Qiagen). .. Paired-ended sequence reads of 250 nt (WC11) or 150 nt (C500 BAC mutants and viruses derived therefrom) were generated using a MiSeq DNA sequencer running version 2 chemistry (Illumina) and assembled using methods described previously [ ] ( ).

    Article Title: A Herpes Simplex Virus Vector System for Expression of Complex Cellular cDNA Libraries ▿
    Article Snippet: .. The DNA was electroporated into GeneHogs bacteria (Invitrogen, Carlsbad, CA) at 2.0 kV, 200 Ω, and 25 μF in a 0.2-cm cuvette, and BAC DNA (DBAC) was purified from Cm-resistant bacteria using a large-construct DNA purification kit (Qiagen, Valencia, CA) with exonuclease digestion. .. The Gateway cassette from Gateway conversion plasmid A (Invitrogen) was modified for this work in the following manner.

    Article Title: Genomic duplication and translocation of reactivation transactivator and bZIP-homolog genes is a conserved event in alcelaphine herpesvirus 1
    Article Snippet: .. Next-generation sequencing BAC clone DNA was purified by using the Large Construct DNA prep kit (Qiagen). .. Viruses were generated from AlHV-1 strain C500 BAC clones in MacT-Cre cells, grown in BT cells, and purified from the supernatant by ultracentrifugation (100,000 × g ) through a 30% (w/v) sucrose cushion for 2 h at 4 °C.

    Article Title: Tissue-specific and neural activity-regulated expression of human BDNF gene in BAC transgenic mice
    Article Snippet: .. In order to validate the reporter activity, BAC DNA was purified using the Large Construct Purification Kit (Qiagen, USA) and transfected into COS-7 cells using DEAE-dextran mediated transfection system [ ]. .. Five days after transfection EGFP expression and distribution in COS-7 cells was visualized using fluorescence microscopy (Eclipse 80i upright microscope, Nikon). hEGFP-BDNF BAC DNA was purified for microinjection by alkaline lysis and linearized with PI-SceI enzyme (NEB, USA).

    Next-Generation Sequencing:

    Article Title: Genomic duplication and translocation of reactivation transactivator and bZIP-homolog genes is a conserved event in alcelaphine herpesvirus 1
    Article Snippet: .. Next-generation sequencing BAC clone DNA was purified by using the Large Construct DNA prep kit (Qiagen). .. Viruses were generated from AlHV-1 strain C500 BAC clones in MacT-Cre cells, grown in BT cells, and purified from the supernatant by ultracentrifugation (100,000 × g ) through a 30% (w/v) sucrose cushion for 2 h at 4 °C.

    Construct:

    Article Title: Alcelaphine herpesvirus 1 genes A7 and A8 regulate viral spread and are essential for malignant catarrhal fever
    Article Snippet: .. BAC clone DNA was purified using the Large Construct DNA prep kit (Qiagen). .. Paired-ended sequence reads of 250 nt (WC11) or 150 nt (C500 BAC mutants and viruses derived therefrom) were generated using a MiSeq DNA sequencer running version 2 chemistry (Illumina) and assembled using methods described previously [ ] ( ).

    Article Title: Genomic duplication and translocation of reactivation transactivator and bZIP-homolog genes is a conserved event in alcelaphine herpesvirus 1
    Article Snippet: .. Next-generation sequencing BAC clone DNA was purified by using the Large Construct DNA prep kit (Qiagen). .. Viruses were generated from AlHV-1 strain C500 BAC clones in MacT-Cre cells, grown in BT cells, and purified from the supernatant by ultracentrifugation (100,000 × g ) through a 30% (w/v) sucrose cushion for 2 h at 4 °C.

    Article Title: Tissue-specific and neural activity-regulated expression of human BDNF gene in BAC transgenic mice
    Article Snippet: .. In order to validate the reporter activity, BAC DNA was purified using the Large Construct Purification Kit (Qiagen, USA) and transfected into COS-7 cells using DEAE-dextran mediated transfection system [ ]. .. Five days after transfection EGFP expression and distribution in COS-7 cells was visualized using fluorescence microscopy (Eclipse 80i upright microscope, Nikon). hEGFP-BDNF BAC DNA was purified for microinjection by alkaline lysis and linearized with PI-SceI enzyme (NEB, USA).

    Purification:

    Article Title: Signal peptide mutations in RANK prevent downstream activation of NF-?B
    Article Snippet: .. The adenoviral DNA, containing the RANK cDNA, was purified using a large-construct DNA purification kit (Qiagen, Crawley, UK). .. Following PacI digestion to linearize the adenoviral DNA, recombinant adenoviruses then were produced by transfecting DNA into HEK293 cells by calcium phosphate precipitation.

    Article Title: Alcelaphine herpesvirus 1 genes A7 and A8 regulate viral spread and are essential for malignant catarrhal fever
    Article Snippet: .. BAC clone DNA was purified using the Large Construct DNA prep kit (Qiagen). .. Paired-ended sequence reads of 250 nt (WC11) or 150 nt (C500 BAC mutants and viruses derived therefrom) were generated using a MiSeq DNA sequencer running version 2 chemistry (Illumina) and assembled using methods described previously [ ] ( ).

    Article Title: A Herpes Simplex Virus Vector System for Expression of Complex Cellular cDNA Libraries ▿
    Article Snippet: .. The DNA was electroporated into GeneHogs bacteria (Invitrogen, Carlsbad, CA) at 2.0 kV, 200 Ω, and 25 μF in a 0.2-cm cuvette, and BAC DNA (DBAC) was purified from Cm-resistant bacteria using a large-construct DNA purification kit (Qiagen, Valencia, CA) with exonuclease digestion. .. The Gateway cassette from Gateway conversion plasmid A (Invitrogen) was modified for this work in the following manner.

    Article Title: Genomic duplication and translocation of reactivation transactivator and bZIP-homolog genes is a conserved event in alcelaphine herpesvirus 1
    Article Snippet: .. Next-generation sequencing BAC clone DNA was purified by using the Large Construct DNA prep kit (Qiagen). .. Viruses were generated from AlHV-1 strain C500 BAC clones in MacT-Cre cells, grown in BT cells, and purified from the supernatant by ultracentrifugation (100,000 × g ) through a 30% (w/v) sucrose cushion for 2 h at 4 °C.

    Article Title: Tissue-specific and neural activity-regulated expression of human BDNF gene in BAC transgenic mice
    Article Snippet: .. In order to validate the reporter activity, BAC DNA was purified using the Large Construct Purification Kit (Qiagen, USA) and transfected into COS-7 cells using DEAE-dextran mediated transfection system [ ]. .. Five days after transfection EGFP expression and distribution in COS-7 cells was visualized using fluorescence microscopy (Eclipse 80i upright microscope, Nikon). hEGFP-BDNF BAC DNA was purified for microinjection by alkaline lysis and linearized with PI-SceI enzyme (NEB, USA).

    Article Title: Evaluation of an Hprt-Luciferase Reporter Gene on a Mammalian Artificial Chromosome in Response to Cytotoxicity
    Article Snippet: .. Principles for recombinase-mediated integration using the MI-MAC system are described elsewhere., The phiC31neoHprt-SLG PAC vector was purified using a Large Construction Kit (Qiagen, Hilden, Germany). .. A9 MI-MAC cells were co-transfected with phiC31neoHprt-SLG and pCAG-phiC31 using Lipofectamine 2000 (Invitrogen) for 6 h ( ).

    Activity Assay:

    Article Title: Tissue-specific and neural activity-regulated expression of human BDNF gene in BAC transgenic mice
    Article Snippet: .. In order to validate the reporter activity, BAC DNA was purified using the Large Construct Purification Kit (Qiagen, USA) and transfected into COS-7 cells using DEAE-dextran mediated transfection system [ ]. .. Five days after transfection EGFP expression and distribution in COS-7 cells was visualized using fluorescence microscopy (Eclipse 80i upright microscope, Nikon). hEGFP-BDNF BAC DNA was purified for microinjection by alkaline lysis and linearized with PI-SceI enzyme (NEB, USA).

    DNA Purification:

    Article Title: Signal peptide mutations in RANK prevent downstream activation of NF-?B
    Article Snippet: .. The adenoviral DNA, containing the RANK cDNA, was purified using a large-construct DNA purification kit (Qiagen, Crawley, UK). .. Following PacI digestion to linearize the adenoviral DNA, recombinant adenoviruses then were produced by transfecting DNA into HEK293 cells by calcium phosphate precipitation.

    Article Title: A Herpes Simplex Virus Vector System for Expression of Complex Cellular cDNA Libraries ▿
    Article Snippet: .. The DNA was electroporated into GeneHogs bacteria (Invitrogen, Carlsbad, CA) at 2.0 kV, 200 Ω, and 25 μF in a 0.2-cm cuvette, and BAC DNA (DBAC) was purified from Cm-resistant bacteria using a large-construct DNA purification kit (Qiagen, Valencia, CA) with exonuclease digestion. .. The Gateway cassette from Gateway conversion plasmid A (Invitrogen) was modified for this work in the following manner.

    Plasmid Preparation:

    Article Title: Evaluation of an Hprt-Luciferase Reporter Gene on a Mammalian Artificial Chromosome in Response to Cytotoxicity
    Article Snippet: .. Principles for recombinase-mediated integration using the MI-MAC system are described elsewhere., The phiC31neoHprt-SLG PAC vector was purified using a Large Construction Kit (Qiagen, Hilden, Germany). .. A9 MI-MAC cells were co-transfected with phiC31neoHprt-SLG and pCAG-phiC31 using Lipofectamine 2000 (Invitrogen) for 6 h ( ).

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  • 80
    Qiagen rnase stock solution
    Rnase Stock Solution, supplied by Qiagen, used in various techniques. Bioz Stars score: 80/100, based on 0 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rnase stock solution/product/Qiagen
    Average 80 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    Qiagen dnase i stock solution
    Dnase I Stock Solution, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dnase i stock solution/product/Qiagen
    Average 99 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
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    Qiagen rnasea stock solution
    Rnasea Stock Solution, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rnasea stock solution/product/Qiagen
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    Qiagen dnase stock solution
    Dnase Stock Solution, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dnase stock solution/product/Qiagen
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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