Structured Review

Thermo Fisher rna
Rna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 3653 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rna/product/Thermo Fisher
Average 99 stars, based on 3653 article reviews
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rna - by Bioz Stars, 2020-01
99/100 stars

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Diagnostic Assay:

Article Title: The natural history of acute Ebola Virus Disease among patients managed in five Ebola treatment units in West Africa: A retrospective cohort study
Article Snippet: In Liberia, the United States Naval Medical Research Center (NMRC) Mobile Laboratory conducted EBOV diagnostic testing for both IMC ETUs using the 1-step quantitative Ebola Zaire real-time reverse transcriptase–polymerase chain reaction (RT-PCR) (TaqMan) assay (Naval Medical Research Center, Frederick, MD). .. Extracted ribonucleic acid was tested for two EBOV gene targets (EBOV Zaire locus and minor groove binding locus) using the Applied Biosystems StepOnePlus instrument.

Amplification:

Article Title: PD-1/PD-L1 expression in non-small-cell lung cancer and its correlation with EGFR/KRAS mutations
Article Snippet: Ribonucleic acid was extracted as per standard protocol after frozen tissues were dissected into TRIzol® (Life Technologies, Carlsbad, CA, USA), and was reverse transcribed into cDNA (cDNA). .. EGFR (exons 18–22) and KRAS (exons 2–3) were amplified using cDNA.

Stable Transfection:

Article Title: PITX2 Modulates Atrial Membrane Potential and the Antiarrhythmic Effects of Sodium-Channel Blockers
Article Snippet: Human embryonic kidney (HEK) 293 cells stably expressing the human Nav 1.5 channel were obtained (SB Ion Channels, Glasgow, UK). .. Ribonucleic acid and complementary deoxyribonucleic acid were synthesized from murine LA, (SuperScript VILO, Thermo Fisher Scientific Inc.) to quantify expression of 20 atrial ion channels and genes with suspected PITX2 -dependent regulation using custom-designed Taqman low density array plates (Thermo Fisher Scientific Inc.).

Synthesized:

Article Title: PITX2 Modulates Atrial Membrane Potential and the Antiarrhythmic Effects of Sodium-Channel Blockers
Article Snippet: .. Ribonucleic acid and complementary deoxyribonucleic acid were synthesized from murine LA, (SuperScript VILO, Thermo Fisher Scientific Inc.) to quantify expression of 20 atrial ion channels and genes with suspected PITX2 -dependent regulation using custom-designed Taqman low density array plates (Thermo Fisher Scientific Inc.). .. Western immunoblotting was performed on murine LA tissue lysates with antibodies detecting TASK-2, Kv 1.6, Na/K ATPase alpha-1, Na/K ATPase alpha-2, Na/Ca exchanger 1, Serca2a, Nav 1.5, or calnexin, using standard methods.

Article Title: Preconditioning of mesenchymal stem cells for improved transplantation efficacy in recessive dystrophic epidermolysis bullosa
Article Snippet: .. All samples used in downstream experiments had an absorbance260/280 ratio exceeding 2.00, and ribonucleic acid concentrations were diluted to 50 to 100 ng/μl prior to cDNA synthesis. cDNA was synthesized using a High Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA, USA). .. Quantitative polymerase chain reaction (qPCR) was carried out using a StepOnePlus Real-Time PCR system (Applied Biosystems).

Article Title: Tolerance to Stress Combination in Tomato Plants: New Insights in the Protective Role of Melatonin
Article Snippet: .. To eliminate traces of DNA, the RNA was treated with DNaseI (Fermentas Life Sciences) according to the manufacturer’s protocol. cDNAs were synthesized from three separate RNAs (2 μg of total RNA) using the SuperScript VILO cDNA synthesis kit (Invitrogen). .. To eliminate traces of DNA, the RNA was treated with DNaseI (Fermentas Life Sciences) according to the manufacturer’s protocol. cDNAs were synthesized from three separate RNAs (2 μg of total RNA) using the SuperScript VILO cDNA synthesis kit (Invitrogen).

Quantitative RT-PCR:

Article Title: Toll-Like Receptor 9 Promotes Cardiac Inflammation and Heart Failure during Polymicrobial Sepsis
Article Snippet: 2.5. mRNA and Protein Detection Inflammatory parameters were detected via RT-qPCR in whole hearts of WT mice 6, 18, 24, and 36 h following CASP (n = 10/group). .. Ribonucleic acid was reversely transcribed using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Darmstadt, Germany; part number 4368814) according to the manufacturer's protocol.

Article Title: Identification of Gene Transcripts Implicated in Peritoneal Membrane Alterations
Article Snippet: .. Ribonucleic acid was then reverse-transcribed using Superscript Reverse transcriptase II (Invitrogen; Thermo Fisher Scientific, Waltham, MA, USA) and the synthetized cDNA was subjected to quantitative reverse transcription (RT-qPCR) analysis. .. Quantitative RT-PCR was performed using a custom-made Taqman low density array (TLDA) (ABI Prism 7900 H.T.

Article Title: Tolerance to Stress Combination in Tomato Plants: New Insights in the Protective Role of Melatonin
Article Snippet: Paragraph title: 4.10. RNA Extraction and qRT-PCR Experiments ... To eliminate traces of DNA, the RNA was treated with DNaseI (Fermentas Life Sciences) according to the manufacturer’s protocol. cDNAs were synthesized from three separate RNAs (2 μg of total RNA) using the SuperScript VILO cDNA synthesis kit (Invitrogen).

SYBR Green Assay:

Article Title: Altered Endometrial Expression of Endothelial Nitric oxide Synthase (eNOS) in women with Unexplained Recurrent Miscarriage and Infertility
Article Snippet: Ribonucleic acid was DNase treated and quantitated by measurement of absorbance in a NanoDrop spectrophotometer. .. The polymerase chain reaction (PCR) mix consisted of 1 mL of 10-fold diluted cDNA, qPCR MasterMix Plus for SYBR green I reagent (GeneON, Germany) and optimized forward and reverse gene-specific primers (300 nmol/L each).

Article Title: Preconditioning of mesenchymal stem cells for improved transplantation efficacy in recessive dystrophic epidermolysis bullosa
Article Snippet: All samples used in downstream experiments had an absorbance260/280 ratio exceeding 2.00, and ribonucleic acid concentrations were diluted to 50 to 100 ng/μl prior to cDNA synthesis. cDNA was synthesized using a High Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA, USA). .. SYBR Green Master Mix reagent (Life Technologies, Grand Island, NY, USA) was used for a fluorescent probe according to the manufacturer’s guidelines.

Microarray:

Article Title: Prognostic Molecular Subtypes of Low-Grade Cancer of the Appendix
Article Snippet: Paragraph title: Ribonucleic acid isolation and microarray analysis ... Ribonucleic acid samples of the validation tumor series were profiled on the Affymetrix GeneAtlas U219 array strips at our Cancer Center’s Cancer Genomics Shared Resource.

TLDA Assay:

Article Title: PITX2 Modulates Atrial Membrane Potential and the Antiarrhythmic Effects of Sodium-Channel Blockers
Article Snippet: .. Ribonucleic acid and complementary deoxyribonucleic acid were synthesized from murine LA, (SuperScript VILO, Thermo Fisher Scientific Inc.) to quantify expression of 20 atrial ion channels and genes with suspected PITX2 -dependent regulation using custom-designed Taqman low density array plates (Thermo Fisher Scientific Inc.). .. Western immunoblotting was performed on murine LA tissue lysates with antibodies detecting TASK-2, Kv 1.6, Na/K ATPase alpha-1, Na/K ATPase alpha-2, Na/Ca exchanger 1, Serca2a, Nav 1.5, or calnexin, using standard methods.

Article Title: Identification of Gene Transcripts Implicated in Peritoneal Membrane Alterations
Article Snippet: Ribonucleic acid was then reverse-transcribed using Superscript Reverse transcriptase II (Invitrogen; Thermo Fisher Scientific, Waltham, MA, USA) and the synthetized cDNA was subjected to quantitative reverse transcription (RT-qPCR) analysis. .. Quantitative RT-PCR was performed using a custom-made Taqman low density array (TLDA) (ABI Prism 7900 H.T.

Expressing:

Article Title: PITX2 Modulates Atrial Membrane Potential and the Antiarrhythmic Effects of Sodium-Channel Blockers
Article Snippet: .. Ribonucleic acid and complementary deoxyribonucleic acid were synthesized from murine LA, (SuperScript VILO, Thermo Fisher Scientific Inc.) to quantify expression of 20 atrial ion channels and genes with suspected PITX2 -dependent regulation using custom-designed Taqman low density array plates (Thermo Fisher Scientific Inc.). .. Western immunoblotting was performed on murine LA tissue lysates with antibodies detecting TASK-2, Kv 1.6, Na/K ATPase alpha-1, Na/K ATPase alpha-2, Na/Ca exchanger 1, Serca2a, Nav 1.5, or calnexin, using standard methods.

Article Title: Toll-Like Receptor 9 Promotes Cardiac Inflammation and Heart Failure during Polymicrobial Sepsis
Article Snippet: Ribonucleic acid was reversely transcribed using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Darmstadt, Germany; part number 4368814) according to the manufacturer's protocol. .. Real-Time qPCR was performed using TaqMan Gene expression Master Mix (Applied Biosystems, part number 4369016) with the following primers: CD14 (Mm00438094_g1), GAPDH (Mm99999915_g1), IL-1β (Mm99999061_g1), IL-6 (Mm01210732_g1), IL-10 (Mm00439616_m1), iNOS (Mm00440485_m1), TLR2 (Mm01213945_g1), TLR4 (Mm00445273_m1), TLR9 (Mm00446193_m1), TNF-α (Mm00443258_m1), and TREM1 (Mm01278455_m1).

Article Title: Identification of Gene Transcripts Implicated in Peritoneal Membrane Alterations
Article Snippet: Omental tissue obtained after the SPARa was dipped into tissue storage reagent RNAlater (Sigma, St. Louis, MO, USA), stored, and transferred at −80°C for further gene expression analysis. .. Ribonucleic acid was then reverse-transcribed using Superscript Reverse transcriptase II (Invitrogen; Thermo Fisher Scientific, Waltham, MA, USA) and the synthetized cDNA was subjected to quantitative reverse transcription (RT-qPCR) analysis.

Article Title: Tolerance to Stress Combination in Tomato Plants: New Insights in the Protective Role of Melatonin
Article Snippet: To eliminate traces of DNA, the RNA was treated with DNaseI (Fermentas Life Sciences) according to the manufacturer’s protocol. cDNAs were synthesized from three separate RNAs (2 μg of total RNA) using the SuperScript VILO cDNA synthesis kit (Invitrogen). .. The accession number of the genes assayed for expression and the primer sequence used are listed in .

Western Blot:

Article Title: PITX2 Modulates Atrial Membrane Potential and the Antiarrhythmic Effects of Sodium-Channel Blockers
Article Snippet: Ribonucleic acid and complementary deoxyribonucleic acid were synthesized from murine LA, (SuperScript VILO, Thermo Fisher Scientific Inc.) to quantify expression of 20 atrial ion channels and genes with suspected PITX2 -dependent regulation using custom-designed Taqman low density array plates (Thermo Fisher Scientific Inc.). .. Western immunoblotting was performed on murine LA tissue lysates with antibodies detecting TASK-2, Kv 1.6, Na/K ATPase alpha-1, Na/K ATPase alpha-2, Na/Ca exchanger 1, Serca2a, Nav 1.5, or calnexin, using standard methods.

Transformation Assay:

Article Title: Prognostic Molecular Subtypes of Low-Grade Cancer of the Appendix
Article Snippet: Ribonucleic acid samples of the validation tumor series were profiled on the Affymetrix GeneAtlas U219 array strips at our Cancer Center’s Cancer Genomics Shared Resource. .. Raw signal-intensity data were normalized and log2 transformed using the Robust Multichip Averaging algorithm as implemented via the simpleaffy package from Bioconductor.

TaqMan microRNA Assay:

Article Title: Regulation of Retinal Inflammation by Rhythmic Expression of MiR-146a in Diabetic Retina
Article Snippet: Ribonucleic acid was isolated using the mirVana miRNA Isolation Kit (Ambion, Austin, TX, USA) according to the manufacturer's instructions. .. Total RNA (10 ng) was used for cDNA synthesis with TaqMan miRNA Assay Reverse Transcription kit according to the manufacturer's instructions.

Reverse Transcription Polymerase Chain Reaction:

Article Title: The natural history of acute Ebola Virus Disease among patients managed in five Ebola treatment units in West Africa: A retrospective cohort study
Article Snippet: In Liberia, the United States Naval Medical Research Center (NMRC) Mobile Laboratory conducted EBOV diagnostic testing for both IMC ETUs using the 1-step quantitative Ebola Zaire real-time reverse transcriptase–polymerase chain reaction (RT-PCR) (TaqMan) assay (Naval Medical Research Center, Frederick, MD). .. Extracted ribonucleic acid was tested for two EBOV gene targets (EBOV Zaire locus and minor groove binding locus) using the Applied Biosystems StepOnePlus instrument.

Article Title: Successful Implementation of a Multicountry Clinical Surveillance and Data Collection System for Ebola Virus Disease in West Africa: Findings and Lessons Learned
Article Snippet: Diagnosis was confirmed with the 1-step quantitative Ebola Zaire real-time reverse transcriptase–polymerase chain reaction (RT-PCR) (TaqMan) assay (NMRC, Frederick, MD). .. Extracted ribonucleic acid was tested for 2 EVD gene targets (Zaire ebolavirus [EBOV] locus and minor groove binding locus), using the Applied Biosystems StepOnePlus instrument.

Generated:

Article Title: Production of a gonadotropin-releasing hormone 2 receptor knockdown (GNRHR2 KD) swine line
Article Snippet: Ribonucleic acid was DNAse treated, quantitated on a Nanodrop spectrophotometer (NanoDrop Technologies, Inc.) and reverse transcribed (2 ug) into cDNA as described above. .. Droplets were generated using the QX200 Droplet Generator (Bio-Rad) according to manufacturer’s instructions.

Polymerase Chain Reaction:

Article Title: Altered Endometrial Expression of Endothelial Nitric oxide Synthase (eNOS) in women with Unexplained Recurrent Miscarriage and Infertility
Article Snippet: Ribonucleic acid was DNase treated and quantitated by measurement of absorbance in a NanoDrop spectrophotometer. .. The polymerase chain reaction (PCR) mix consisted of 1 mL of 10-fold diluted cDNA, qPCR MasterMix Plus for SYBR green I reagent (GeneON, Germany) and optimized forward and reverse gene-specific primers (300 nmol/L each).

Article Title: Toll-Like Receptor 9 Promotes Cardiac Inflammation and Heart Failure during Polymicrobial Sepsis
Article Snippet: Ribonucleic acid was reversely transcribed using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Darmstadt, Germany; part number 4368814) according to the manufacturer's protocol. .. The reaction was processed in a TaqMan PCR system (Applied Biosystems), and the results were analysed based on the ratio between target accumulation and GAPDH accumulation.

Article Title: Production of a gonadotropin-releasing hormone 2 receptor knockdown (GNRHR2 KD) swine line
Article Snippet: Paragraph title: Digital droplet PCR ... Ribonucleic acid was DNAse treated, quantitated on a Nanodrop spectrophotometer (NanoDrop Technologies, Inc.) and reverse transcribed (2 ug) into cDNA as described above.

Article Title: Prevalence of hepatitis delta virus infection among hepatitis b virus surface antigen positive patients circulating in the largest province of pakistan
Article Snippet: .. Abbreviations HDV: hepatitis delta virus; HBV: hepatitis B virus; HCC: hepatocellular carcinoma; HBSAG: hepatitis B surface antigen; ELISA: enzyme linked immunosorbent assay; CDNA: complementary DNA; RNA: ribonucleic acid; M-MLV RTase: Moloney Murine Leukemia Virus reverse transcriptase; ABI: Applied Biosystems Inc.; PCR: polymerase chain reaction. ..

Article Title: PD-1/PD-L1 expression in non-small-cell lung cancer and its correlation with EGFR/KRAS mutations
Article Snippet: EGFR mutations and KRAS mutations were evaluated using polymerase chain reaction and direct sequencing. .. Ribonucleic acid was extracted as per standard protocol after frozen tissues were dissected into TRIzol® (Life Technologies, Carlsbad, CA, USA), and was reverse transcribed into cDNA (cDNA).

Binding Assay:

Article Title: The natural history of acute Ebola Virus Disease among patients managed in five Ebola treatment units in West Africa: A retrospective cohort study
Article Snippet: .. Extracted ribonucleic acid was tested for two EBOV gene targets (EBOV Zaire locus and minor groove binding locus) using the Applied Biosystems StepOnePlus instrument. ..

Article Title: Successful Implementation of a Multicountry Clinical Surveillance and Data Collection System for Ebola Virus Disease in West Africa: Findings and Lessons Learned
Article Snippet: .. Extracted ribonucleic acid was tested for 2 EVD gene targets (Zaire ebolavirus [EBOV] locus and minor groove binding locus), using the Applied Biosystems StepOnePlus instrument. ..

Mutagenesis:

Article Title: PD-1/PD-L1 expression in non-small-cell lung cancer and its correlation with EGFR/KRAS mutations
Article Snippet: Paragraph title: Mutation analysis of EGFR and KRAS ... Ribonucleic acid was extracted as per standard protocol after frozen tissues were dissected into TRIzol® (Life Technologies, Carlsbad, CA, USA), and was reverse transcribed into cDNA (cDNA).

Isolation:

Article Title: Altered Endometrial Expression of Endothelial Nitric oxide Synthase (eNOS) in women with Unexplained Recurrent Miscarriage and Infertility
Article Snippet: RNA was isolated using the High Pure RNA Isolation Kit (Roche applied science). .. Ribonucleic acid was DNase treated and quantitated by measurement of absorbance in a NanoDrop spectrophotometer.

Article Title: Prognostic Molecular Subtypes of Low-Grade Cancer of the Appendix
Article Snippet: Paragraph title: Ribonucleic acid isolation and microarray analysis ... Ribonucleic acid samples of the validation tumor series were profiled on the Affymetrix GeneAtlas U219 array strips at our Cancer Center’s Cancer Genomics Shared Resource.

Article Title: Toll-Like Receptor 9 Promotes Cardiac Inflammation and Heart Failure during Polymicrobial Sepsis
Article Snippet: For RNA extraction the hearts were homogenized and RNA was isolated using the RNeasy Mini kit (Qiagen, Hilden, Germany). .. Ribonucleic acid was reversely transcribed using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Darmstadt, Germany; part number 4368814) according to the manufacturer's protocol.

Article Title: Regulation of Retinal Inflammation by Rhythmic Expression of MiR-146a in Diabetic Retina
Article Snippet: .. Ribonucleic acid was isolated using the mirVana miRNA Isolation Kit (Ambion, Austin, TX, USA) according to the manufacturer's instructions. .. The purity and quantity of RNA were assessed using the NanoDrop ND-1000 spectrophotometer (Thermo Scientific, Wilmington, DE, USA).

Article Title: Expression of Hepatic Cytochrome P450s in Rats Administered with Guibi-tang, a Traditional Herbal Formula
Article Snippet: .. Isolation of total ribonucleic acid and cDNA synthesis The total RNA was isolated from livers using TriZol reagent (Invitrogen, Camarillo, CA, USA) according to the manufacturer's instructions. ..

Article Title: Identification of Gene Transcripts Implicated in Peritoneal Membrane Alterations
Article Snippet: Paragraph title: RNA Isolation and Real-Time Quantitative RT-qPCR ... Ribonucleic acid was then reverse-transcribed using Superscript Reverse transcriptase II (Invitrogen; Thermo Fisher Scientific, Waltham, MA, USA) and the synthetized cDNA was subjected to quantitative reverse transcription (RT-qPCR) analysis.

Article Title: Tolerance to Stress Combination in Tomato Plants: New Insights in the Protective Role of Melatonin
Article Snippet: RNA Extraction and qRT-PCR Experiments Total RNA was isolated from whole tomato leaves using the TRI Reagent (Sigma-Aldrich, Ref. T9424). .. To eliminate traces of DNA, the RNA was treated with DNaseI (Fermentas Life Sciences) according to the manufacturer’s protocol. cDNAs were synthesized from three separate RNAs (2 μg of total RNA) using the SuperScript VILO cDNA synthesis kit (Invitrogen).

Labeling:

Article Title: Prognostic Molecular Subtypes of Low-Grade Cancer of the Appendix
Article Snippet: Ribonucleic acid samples of the validation tumor series were profiled on the Affymetrix GeneAtlas U219 array strips at our Cancer Center’s Cancer Genomics Shared Resource. .. Briefly, 250 ng total RNA was used to generate labeled in vitro transcription products (complementary RNA).

Article Title: Production of a gonadotropin-releasing hormone 2 receptor knockdown (GNRHR2 KD) swine line
Article Snippet: Ribonucleic acid was DNAse treated, quantitated on a Nanodrop spectrophotometer (NanoDrop Technologies, Inc.) and reverse transcribed (2 ug) into cDNA as described above. .. To quantify GNRHR2 transcript levels, specific primers (Integrated DNA Technologies) and a FAM labeled TaqMan MGB probe (Applied Biosystems) were utilized as described above.

Mouse Assay:

Article Title: Toll-Like Receptor 9 Promotes Cardiac Inflammation and Heart Failure during Polymicrobial Sepsis
Article Snippet: 2.5. mRNA and Protein Detection Inflammatory parameters were detected via RT-qPCR in whole hearts of WT mice 6, 18, 24, and 36 h following CASP (n = 10/group). .. Ribonucleic acid was reversely transcribed using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Darmstadt, Germany; part number 4368814) according to the manufacturer's protocol.

Sequencing:

Article Title: Identification of Gene Transcripts Implicated in Peritoneal Membrane Alterations
Article Snippet: Ribonucleic acid was then reverse-transcribed using Superscript Reverse transcriptase II (Invitrogen; Thermo Fisher Scientific, Waltham, MA, USA) and the synthetized cDNA was subjected to quantitative reverse transcription (RT-qPCR) analysis. .. Sequence Detection system; Applied Biosystems, Thermo Fisher Scientific, Waltham, MA, USA) with fast protocol to analyze 45 selected target genes chosen on the basis of relevance to the study.

Article Title: PD-1/PD-L1 expression in non-small-cell lung cancer and its correlation with EGFR/KRAS mutations
Article Snippet: EGFR mutations and KRAS mutations were evaluated using polymerase chain reaction and direct sequencing. .. Ribonucleic acid was extracted as per standard protocol after frozen tissues were dissected into TRIzol® (Life Technologies, Carlsbad, CA, USA), and was reverse transcribed into cDNA (cDNA).

Article Title: Tolerance to Stress Combination in Tomato Plants: New Insights in the Protective Role of Melatonin
Article Snippet: To eliminate traces of DNA, the RNA was treated with DNaseI (Fermentas Life Sciences) according to the manufacturer’s protocol. cDNAs were synthesized from three separate RNAs (2 μg of total RNA) using the SuperScript VILO cDNA synthesis kit (Invitrogen). .. The accession number of the genes assayed for expression and the primer sequence used are listed in .

TaqMan Assay:

Article Title: The natural history of acute Ebola Virus Disease among patients managed in five Ebola treatment units in West Africa: A retrospective cohort study
Article Snippet: In Liberia, the United States Naval Medical Research Center (NMRC) Mobile Laboratory conducted EBOV diagnostic testing for both IMC ETUs using the 1-step quantitative Ebola Zaire real-time reverse transcriptase–polymerase chain reaction (RT-PCR) (TaqMan) assay (Naval Medical Research Center, Frederick, MD). .. Extracted ribonucleic acid was tested for two EBOV gene targets (EBOV Zaire locus and minor groove binding locus) using the Applied Biosystems StepOnePlus instrument.

Article Title: Successful Implementation of a Multicountry Clinical Surveillance and Data Collection System for Ebola Virus Disease in West Africa: Findings and Lessons Learned
Article Snippet: Diagnosis was confirmed with the 1-step quantitative Ebola Zaire real-time reverse transcriptase–polymerase chain reaction (RT-PCR) (TaqMan) assay (NMRC, Frederick, MD). .. Extracted ribonucleic acid was tested for 2 EVD gene targets (Zaire ebolavirus [EBOV] locus and minor groove binding locus), using the Applied Biosystems StepOnePlus instrument.

Purification:

Article Title: Prognostic Molecular Subtypes of Low-Grade Cancer of the Appendix
Article Snippet: Ribonucleic acid samples of the validation tumor series were profiled on the Affymetrix GeneAtlas U219 array strips at our Cancer Center’s Cancer Genomics Shared Resource. .. The complementary RNA was purified, hybridized to the array, washed, and scanned according to GeneAtlas standard operating procedures.

Software:

Article Title: Tolerance to Stress Combination in Tomato Plants: New Insights in the Protective Role of Melatonin
Article Snippet: To eliminate traces of DNA, the RNA was treated with DNaseI (Fermentas Life Sciences) according to the manufacturer’s protocol. cDNAs were synthesized from three separate RNAs (2 μg of total RNA) using the SuperScript VILO cDNA synthesis kit (Invitrogen). .. Primer3 software (Thermo Fisher Scientific Inc., Waltham, MA, USA) was used for primer design based on the tomato sequences available at Sol Genomics Network ( http://solgenomics.net/ ).

Real-time Polymerase Chain Reaction:

Article Title: Altered Endometrial Expression of Endothelial Nitric oxide Synthase (eNOS) in women with Unexplained Recurrent Miscarriage and Infertility
Article Snippet: Ribonucleic acid was DNase treated and quantitated by measurement of absorbance in a NanoDrop spectrophotometer. .. The polymerase chain reaction (PCR) mix consisted of 1 mL of 10-fold diluted cDNA, qPCR MasterMix Plus for SYBR green I reagent (GeneON, Germany) and optimized forward and reverse gene-specific primers (300 nmol/L each).

Article Title: Toll-Like Receptor 9 Promotes Cardiac Inflammation and Heart Failure during Polymicrobial Sepsis
Article Snippet: Ribonucleic acid was reversely transcribed using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Darmstadt, Germany; part number 4368814) according to the manufacturer's protocol. .. Real-Time qPCR was performed using TaqMan Gene expression Master Mix (Applied Biosystems, part number 4369016) with the following primers: CD14 (Mm00438094_g1), GAPDH (Mm99999915_g1), IL-1β (Mm99999061_g1), IL-6 (Mm01210732_g1), IL-10 (Mm00439616_m1), iNOS (Mm00440485_m1), TLR2 (Mm01213945_g1), TLR4 (Mm00445273_m1), TLR9 (Mm00446193_m1), TNF-α (Mm00443258_m1), and TREM1 (Mm01278455_m1).

Article Title: Preconditioning of mesenchymal stem cells for improved transplantation efficacy in recessive dystrophic epidermolysis bullosa
Article Snippet: Paragraph title: RNA extraction, reverse transcription, and quantitative polymerase chain reaction ... All samples used in downstream experiments had an absorbance260/280 ratio exceeding 2.00, and ribonucleic acid concentrations were diluted to 50 to 100 ng/μl prior to cDNA synthesis. cDNA was synthesized using a High Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA, USA).

RNA Extraction:

Article Title: Toll-Like Receptor 9 Promotes Cardiac Inflammation and Heart Failure during Polymicrobial Sepsis
Article Snippet: For RNA extraction the hearts were homogenized and RNA was isolated using the RNeasy Mini kit (Qiagen, Hilden, Germany). .. Ribonucleic acid was reversely transcribed using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Darmstadt, Germany; part number 4368814) according to the manufacturer's protocol.

Article Title: Preconditioning of mesenchymal stem cells for improved transplantation efficacy in recessive dystrophic epidermolysis bullosa
Article Snippet: Paragraph title: RNA extraction, reverse transcription, and quantitative polymerase chain reaction ... All samples used in downstream experiments had an absorbance260/280 ratio exceeding 2.00, and ribonucleic acid concentrations were diluted to 50 to 100 ng/μl prior to cDNA synthesis. cDNA was synthesized using a High Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA, USA).

Article Title: Tolerance to Stress Combination in Tomato Plants: New Insights in the Protective Role of Melatonin
Article Snippet: Paragraph title: 4.10. RNA Extraction and qRT-PCR Experiments ... To eliminate traces of DNA, the RNA was treated with DNaseI (Fermentas Life Sciences) according to the manufacturer’s protocol. cDNAs were synthesized from three separate RNAs (2 μg of total RNA) using the SuperScript VILO cDNA synthesis kit (Invitrogen).

In Vitro:

Article Title: Prognostic Molecular Subtypes of Low-Grade Cancer of the Appendix
Article Snippet: Ribonucleic acid samples of the validation tumor series were profiled on the Affymetrix GeneAtlas U219 array strips at our Cancer Center’s Cancer Genomics Shared Resource. .. Briefly, 250 ng total RNA was used to generate labeled in vitro transcription products (complementary RNA).

Transgenic Assay:

Article Title: Production of a gonadotropin-releasing hormone 2 receptor knockdown (GNRHR2 KD) swine line
Article Snippet: Digital droplet PCR Digital droplet PCR (ddPCR) was used to quantify the abundance of GNRHR2 mRNA in testes of transgenic (n = 5) and control (n = 4) boars. .. Ribonucleic acid was DNAse treated, quantitated on a Nanodrop spectrophotometer (NanoDrop Technologies, Inc.) and reverse transcribed (2 ug) into cDNA as described above.

Enzyme-linked Immunosorbent Assay:

Article Title: Toll-Like Receptor 9 Promotes Cardiac Inflammation and Heart Failure during Polymicrobial Sepsis
Article Snippet: Ribonucleic acid was reversely transcribed using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Darmstadt, Germany; part number 4368814) according to the manufacturer's protocol. .. ELISA was performed using Quantikine Mouse kits (R & D System, Abingdon, UK) for IL-1β (MLB00B) and IL-6 (M6000B) according to the manufacturer's protocol (n = 10/group).

Article Title: Prevalence of hepatitis delta virus infection among hepatitis b virus surface antigen positive patients circulating in the largest province of pakistan
Article Snippet: .. Abbreviations HDV: hepatitis delta virus; HBV: hepatitis B virus; HCC: hepatocellular carcinoma; HBSAG: hepatitis B surface antigen; ELISA: enzyme linked immunosorbent assay; CDNA: complementary DNA; RNA: ribonucleic acid; M-MLV RTase: Moloney Murine Leukemia Virus reverse transcriptase; ABI: Applied Biosystems Inc.; PCR: polymerase chain reaction. ..

Spectrophotometry:

Article Title: Altered Endometrial Expression of Endothelial Nitric oxide Synthase (eNOS) in women with Unexplained Recurrent Miscarriage and Infertility
Article Snippet: .. Ribonucleic acid was DNase treated and quantitated by measurement of absorbance in a NanoDrop spectrophotometer. .. One microgram of total RNA was reverse transcribed into single stranded complementary DNA (cDNA) with use of the Omniscript Reverse Transcription kit (GeneON, Germany) at 37°C for 60 minutes in a total volume of 20 uL.

Article Title: Regulation of Retinal Inflammation by Rhythmic Expression of MiR-146a in Diabetic Retina
Article Snippet: Ribonucleic acid was isolated using the mirVana miRNA Isolation Kit (Ambion, Austin, TX, USA) according to the manufacturer's instructions. .. The purity and quantity of RNA were assessed using the NanoDrop ND-1000 spectrophotometer (Thermo Scientific, Wilmington, DE, USA).

Article Title: Production of a gonadotropin-releasing hormone 2 receptor knockdown (GNRHR2 KD) swine line
Article Snippet: .. Ribonucleic acid was DNAse treated, quantitated on a Nanodrop spectrophotometer (NanoDrop Technologies, Inc.) and reverse transcribed (2 ug) into cDNA as described above. .. To quantify GNRHR2 transcript levels, specific primers (Integrated DNA Technologies) and a FAM labeled TaqMan MGB probe (Applied Biosystems) were utilized as described above.

Article Title: Identification of Gene Transcripts Implicated in Peritoneal Membrane Alterations
Article Snippet: Quantification of total RNA and control of purity were performed by means of spectrophotometry method (NanoDrop 2000; Thermo Fisher Scientific, Waltham, MA, USA). .. Ribonucleic acid was then reverse-transcribed using Superscript Reverse transcriptase II (Invitrogen; Thermo Fisher Scientific, Waltham, MA, USA) and the synthetized cDNA was subjected to quantitative reverse transcription (RT-qPCR) analysis.

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