rna stabilization  (Qiagen)


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    Structured Review

    Qiagen rna stabilization
    Rna Stabilization, supplied by Qiagen, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rna stabilization/product/Qiagen
    Average 93 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    rna stabilization - by Bioz Stars, 2020-04
    93/100 stars

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    Related Articles

    Centrifugation:

    Article Title: Establishment of an Analytical System for the Human Fecal Microbiota, Based on Reverse Transcription-Quantitative PCR Targeting of Multicopy rRNA Molecules ▿Establishment of an Analytical System for the Human Fecal Microbiota, Based on Reverse Transcription-Quantitative PCR Targeting of Multicopy rRNA Molecules ▿ †
    Article Snippet: For RNA stabilization, fresh cultures of each bacterial (50 μl) or fecal (200 μl) homogenate were added to 2 volumes of RNAprotect bacterial reagent (Qiagen GmbH, Hilden, Germany) and then incubated for 5 min at room temperature. .. After the centrifugation of the mixture at 5,000 × g for 10 min, the supernatant was discarded, and pellets were stored at −80°C until used to extract RNA.

    Article Title: Heat Resistance and Salt Hypersensitivity in Lactococcus lactis Due to Spontaneous Mutation of llmg_1816 (gdpP) Induced by High-Temperature Growth
    Article Snippet: .. Early-log-phase (OD600 = 0.2) cultures were first subjected to RNA stabilization with RNA protect (Qiagen, Hilden, Germany) and harvested by centrifugation (16,000 × g , 5 min), and RNA was extracted using the RNeasy minikit (Qiagen, Hilden, Germany). .. DNA contamination was removed using 2U DNase I (NEB, Ipswich, MA).

    Amplification:

    Article Title: Involvement of the different lung compartments in the pathogenesis of pH1N1 influenza virus infection in ferrets
    Article Snippet: Amplicon sizes of the target genes range between 90 and 120 base pairs. .. RNA extraction was performed on the ferret lung tissue samples with an RNeasy Mini Kit (Qiagen), using the RNA stabilization and on-column DNase digestion protocols (Qiagen).

    Article Title: Leapfrog diagnostics: Demonstration of a broad spectrum pathogen identification platform in a resource-limited setting
    Article Snippet: Reverse transcription and real time PCR (RT-PCR) In order to detect influenza B RNA in testing RNA stabilization on FTA 1 mm processed disks were placed in a 0.5 mL PCR tube, and subjected to one step reverse transcription and PCR (RT-PCR) using Qiagen OneStep RT-PCR Kit (Qiagen, Valencia, CA) according to the manufacturer’s instructions. .. The expected amplicon was a 162 bp segment of the influenza B matrix gene.

    Cycling Probe Technology:

    Article Title: A 3-day EGCG-supplementation reduces interstitial lactate concentration in skeletal muscle of overweight subjects
    Article Snippet: Total RNA was isolated, using the total RNA stabilization and purification kit for human samples Qiagen (Qiagen, Hombrechtikon, Switzerland). .. Gene expression of HSL, ATGL, CPT-1, ACC-1, FAT/CD36 and leptin was measured by the Taqman multiplex method using the ABI 7900 quantitative real-time RT-PCR instrument (Applied Biosystems, Rotkreuz, Switzerland) as described by Heim .

    Quantitative RT-PCR:

    Article Title: Involvement of the different lung compartments in the pathogenesis of pH1N1 influenza virus infection in ferrets
    Article Snippet: Briefly, RRT-PCR was performed using a Power SYBR green kit (Applied Biosystems) and Fast 7500 equipment (Applied Biosystems). .. RNA extraction was performed on the ferret lung tissue samples with an RNeasy Mini Kit (Qiagen), using the RNA stabilization and on-column DNase digestion protocols (Qiagen).

    Article Title: A 3-day EGCG-supplementation reduces interstitial lactate concentration in skeletal muscle of overweight subjects
    Article Snippet: Total RNA was isolated, using the total RNA stabilization and purification kit for human samples Qiagen (Qiagen, Hombrechtikon, Switzerland). .. Gene expression of HSL, ATGL, CPT-1, ACC-1, FAT/CD36 and leptin was measured by the Taqman multiplex method using the ABI 7900 quantitative real-time RT-PCR instrument (Applied Biosystems, Rotkreuz, Switzerland) as described by Heim .

    Article Title: Oligodendrocytes control potassium accumulation in white matter and seizure susceptibility
    Article Snippet: Paragraph title: Fluorescence-activated cell sorting and RT-qPCR ... RNA stabilization, genomic DNA elimination, and reverse transcription were performed using the FastLane Cell cDNA Kit (Qiagen, Hilden, Germany), following the manufacturer’s instructions.

    SYBR Green Assay:

    Article Title: Involvement of the different lung compartments in the pathogenesis of pH1N1 influenza virus infection in ferrets
    Article Snippet: Briefly, RRT-PCR was performed using a Power SYBR green kit (Applied Biosystems) and Fast 7500 equipment (Applied Biosystems). .. RNA extraction was performed on the ferret lung tissue samples with an RNeasy Mini Kit (Qiagen), using the RNA stabilization and on-column DNase digestion protocols (Qiagen).

    Article Title: Oligodendrocytes control potassium accumulation in white matter and seizure susceptibility
    Article Snippet: RNA stabilization, genomic DNA elimination, and reverse transcription were performed using the FastLane Cell cDNA Kit (Qiagen, Hilden, Germany), following the manufacturer’s instructions. .. Real time qPCR was performed using the QuantiTect SYBR Green PCR Kit (Qiagen), following the manufacturer’s instructions, and using a StepOnePlus Real-Time PCR System (Applied Biosystems, Foster City, CA).

    Article Title: Heat Resistance and Salt Hypersensitivity in Lactococcus lactis Due to Spontaneous Mutation of llmg_1816 (gdpP) Induced by High-Temperature Growth
    Article Snippet: Early-log-phase (OD600 = 0.2) cultures were first subjected to RNA stabilization with RNA protect (Qiagen, Hilden, Germany) and harvested by centrifugation (16,000 × g , 5 min), and RNA was extracted using the RNeasy minikit (Qiagen, Hilden, Germany). .. Platinum SYBR green quantitative PCR (qPCR) SuperMix-UDG (Invitrogen, Carlsbad, CA) was used for qPCR.

    Incubation:

    Article Title: Establishment of an Analytical System for the Human Fecal Microbiota, Based on Reverse Transcription-Quantitative PCR Targeting of Multicopy rRNA Molecules ▿Establishment of an Analytical System for the Human Fecal Microbiota, Based on Reverse Transcription-Quantitative PCR Targeting of Multicopy rRNA Molecules ▿ †
    Article Snippet: .. For RNA stabilization, fresh cultures of each bacterial (50 μl) or fecal (200 μl) homogenate were added to 2 volumes of RNAprotect bacterial reagent (Qiagen GmbH, Hilden, Germany) and then incubated for 5 min at room temperature. .. After the centrifugation of the mixture at 5,000 × g for 10 min, the supernatant was discarded, and pellets were stored at −80°C until used to extract RNA.

    Article Title: Quorum Sensing Modulates the Epibiotic-Parasitic Relationship Between Actinomyces odontolyticus and Its Saccharibacteria epibiont, a Nanosynbacter lyticus Strain, TM7x
    Article Snippet: RNA Isolation and cDNA Synthesis RNA isolation and cDNA synthesis protocols were performed as previously reported ( ) with the following modifications: cells were harvested from a 50 mL culture in BHI under 2% microaerophilic conditions after 18 h of incubation. .. Prior to RNA isolation, cell pellets were treated for RNA stabilization with RNA protect Cell Reagent (Qiagen, Cat. No./ID 76526).

    Article Title: The Role of Flagella in Clostridium difficile Pathogenesis: Comparison between a Non-Epidemic and an Epidemic Strain
    Article Snippet: RNA stabilization was achieved by using RNAprotect Bacteria Reagent and an RNeasy Mini Kit (Qiagen) as described by the manufacturer's instructions. .. The total-RNA was dissolved in 50 µl of RNase-free water (DEPC-treated water) and DNase I digestion was then carried out to remove contaminating DNA in RNA samples by adding TURBO DNase buffer and TURBO DNase (Ambion) and incubated at 37°C for 1 h, according to the manufacturer's instructions.

    Formalin-fixed Paraffin-Embedded:

    Article Title: Involvement of the different lung compartments in the pathogenesis of pH1N1 influenza virus infection in ferrets
    Article Snippet: Gene expression profiles Total RNA was isolated from LCM tissues, using the miRNeasy FFPE Kit (no. 217504, Qiagen), as described in the above section. .. RNA extraction was performed on the ferret lung tissue samples with an RNeasy Mini Kit (Qiagen), using the RNA stabilization and on-column DNase digestion protocols (Qiagen).

    Expressing:

    Article Title: Involvement of the different lung compartments in the pathogenesis of pH1N1 influenza virus infection in ferrets
    Article Snippet: Paragraph title: Gene expression profiles ... RNA extraction was performed on the ferret lung tissue samples with an RNeasy Mini Kit (Qiagen), using the RNA stabilization and on-column DNase digestion protocols (Qiagen).

    Article Title: Flexible Metabolism and Suppression of Latent Enzymes Are Important for Escherichia coli Adaptation to Diverse Environments within the Host
    Article Snippet: For RNA stabilization, a 2:1 (vol/vol) ratio of RNA protect (Qiagen) was added to 250 μl of bacterial sample and spun down (Thermo Scientific Legend Micro 21, room temperature, 5 min, 9,600 × g ). .. Then, 5 ng of total cDNA was added to each reaction, and qPCR was performed with SyberGreen PowerUp reagent (Invitrogen) with primers to amplify the following genes: fliA , RNA polymerase sigma factor for flagellar operon; fliC , flagellin assembly; fimA , type 1 fimbrial protein; and papA and papA2 , major pilin subunit A. gapA expression was used as the internal control to calculate relative expression values of experimental genes.

    Article Title: A 3-day EGCG-supplementation reduces interstitial lactate concentration in skeletal muscle of overweight subjects
    Article Snippet: Total RNA was isolated, using the total RNA stabilization and purification kit for human samples Qiagen (Qiagen, Hombrechtikon, Switzerland). .. Gene expression of HSL, ATGL, CPT-1, ACC-1, FAT/CD36 and leptin was measured by the Taqman multiplex method using the ABI 7900 quantitative real-time RT-PCR instrument (Applied Biosystems, Rotkreuz, Switzerland) as described by Heim .

    Modification:

    Article Title: Analysis of gene expression in mouse brain regions after exposure to 1.9 GHz radiofrequency fields
    Article Snippet: For tissue disruption and RNA stabilization, each sample was treated consecutively, as follows: a 350 μ l aliquot of RLT buffer (Qiagen Inc.) containing 1% β-mercaptoethanol was added directly onto the frozen tissue sample and then immediately sonicated (Micro Ultrasonic Cell Disruptor, Kontes Inc.) for 5 s in the microtube with a sterilized probe treated with RNase-Zap, followed immediately by homogenization in the microtube for 30 s using a handheld homogenizer (Pellet Pestle Motor, Kontes Inc.) with disposable RNase/DNase-free pestles (Kimble-Chase Inc.). .. Thereafter, the samples were processed as a group using RNeasy Micro kits (Qiagen Inc.), with on-column DNase digestion, using a modified version of the manufacturer’s instructions (e.g., additional RW1 and RPE washes).

    Article Title: Establishment of an Analytical System for the Human Fecal Microbiota, Based on Reverse Transcription-Quantitative PCR Targeting of Multicopy rRNA Molecules ▿Establishment of an Analytical System for the Human Fecal Microbiota, Based on Reverse Transcription-Quantitative PCR Targeting of Multicopy rRNA Molecules ▿ †
    Article Snippet: For RNA stabilization, fresh cultures of each bacterial (50 μl) or fecal (200 μl) homogenate were added to 2 volumes of RNAprotect bacterial reagent (Qiagen GmbH, Hilden, Germany) and then incubated for 5 min at room temperature. .. RNA was isolated using a modified method of acid guanidinium thiocyanate-phenol-chloroform extraction ( ).

    Real-time Polymerase Chain Reaction:

    Article Title: Flexible Metabolism and Suppression of Latent Enzymes Are Important for Escherichia coli Adaptation to Diverse Environments within the Host
    Article Snippet: Paragraph title: qPCR. ... For RNA stabilization, a 2:1 (vol/vol) ratio of RNA protect (Qiagen) was added to 250 μl of bacterial sample and spun down (Thermo Scientific Legend Micro 21, room temperature, 5 min, 9,600 × g ).

    Article Title: Leapfrog diagnostics: Demonstration of a broad spectrum pathogen identification platform in a resource-limited setting
    Article Snippet: .. Reverse transcription and real time PCR (RT-PCR) In order to detect influenza B RNA in testing RNA stabilization on FTA 1 mm processed disks were placed in a 0.5 mL PCR tube, and subjected to one step reverse transcription and PCR (RT-PCR) using Qiagen OneStep RT-PCR Kit (Qiagen, Valencia, CA) according to the manufacturer’s instructions. ..

    Article Title: Oligodendrocytes control potassium accumulation in white matter and seizure susceptibility
    Article Snippet: RNA stabilization, genomic DNA elimination, and reverse transcription were performed using the FastLane Cell cDNA Kit (Qiagen, Hilden, Germany), following the manufacturer’s instructions. .. Real time qPCR was performed using the QuantiTect SYBR Green PCR Kit (Qiagen), following the manufacturer’s instructions, and using a StepOnePlus Real-Time PCR System (Applied Biosystems, Foster City, CA).

    Article Title: Heat Resistance and Salt Hypersensitivity in Lactococcus lactis Due to Spontaneous Mutation of llmg_1816 (gdpP) Induced by High-Temperature Growth
    Article Snippet: Paragraph title: RNA isolation, cDNA synthesis, and qPCR. ... Early-log-phase (OD600 = 0.2) cultures were first subjected to RNA stabilization with RNA protect (Qiagen, Hilden, Germany) and harvested by centrifugation (16,000 × g , 5 min), and RNA was extracted using the RNeasy minikit (Qiagen, Hilden, Germany).

    Generated:

    Article Title: Oligodendrocytes control potassium accumulation in white matter and seizure susceptibility
    Article Snippet: Brains were isolated and single-cell suspensions were generated using the Neural Tissue Dissociation Kit (Miltenyi Biotec, Bergisch Gladbach, Germany), following the manufacturer’s instructions. .. RNA stabilization, genomic DNA elimination, and reverse transcription were performed using the FastLane Cell cDNA Kit (Qiagen, Hilden, Germany), following the manufacturer’s instructions.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Leapfrog diagnostics: Demonstration of a broad spectrum pathogen identification platform in a resource-limited setting
    Article Snippet: .. Reverse transcription and real time PCR (RT-PCR) In order to detect influenza B RNA in testing RNA stabilization on FTA 1 mm processed disks were placed in a 0.5 mL PCR tube, and subjected to one step reverse transcription and PCR (RT-PCR) using Qiagen OneStep RT-PCR Kit (Qiagen, Valencia, CA) according to the manufacturer’s instructions. ..

    Sonication:

    Article Title: Analysis of gene expression in mouse brain regions after exposure to 1.9 GHz radiofrequency fields
    Article Snippet: .. For tissue disruption and RNA stabilization, each sample was treated consecutively, as follows: a 350 μ l aliquot of RLT buffer (Qiagen Inc.) containing 1% β-mercaptoethanol was added directly onto the frozen tissue sample and then immediately sonicated (Micro Ultrasonic Cell Disruptor, Kontes Inc.) for 5 s in the microtube with a sterilized probe treated with RNase-Zap, followed immediately by homogenization in the microtube for 30 s using a handheld homogenizer (Pellet Pestle Motor, Kontes Inc.) with disposable RNase/DNase-free pestles (Kimble-Chase Inc.). ..

    Fluorescence:

    Article Title: Oligodendrocytes control potassium accumulation in white matter and seizure susceptibility
    Article Snippet: Paragraph title: Fluorescence-activated cell sorting and RT-qPCR ... RNA stabilization, genomic DNA elimination, and reverse transcription were performed using the FastLane Cell cDNA Kit (Qiagen, Hilden, Germany), following the manufacturer’s instructions.

    Isolation:

    Article Title: Analysis of gene expression in mouse brain regions after exposure to 1.9 GHz radiofrequency fields
    Article Snippet: Paragraph title: RNA isolation and labelling ... For tissue disruption and RNA stabilization, each sample was treated consecutively, as follows: a 350 μ l aliquot of RLT buffer (Qiagen Inc.) containing 1% β-mercaptoethanol was added directly onto the frozen tissue sample and then immediately sonicated (Micro Ultrasonic Cell Disruptor, Kontes Inc.) for 5 s in the microtube with a sterilized probe treated with RNase-Zap, followed immediately by homogenization in the microtube for 30 s using a handheld homogenizer (Pellet Pestle Motor, Kontes Inc.) with disposable RNase/DNase-free pestles (Kimble-Chase Inc.).

    Article Title: Involvement of the different lung compartments in the pathogenesis of pH1N1 influenza virus infection in ferrets
    Article Snippet: Gene expression profiles Total RNA was isolated from LCM tissues, using the miRNeasy FFPE Kit (no. 217504, Qiagen), as described in the above section. .. RNA extraction was performed on the ferret lung tissue samples with an RNeasy Mini Kit (Qiagen), using the RNA stabilization and on-column DNase digestion protocols (Qiagen).

    Article Title: Flexible Metabolism and Suppression of Latent Enzymes Are Important for Escherichia coli Adaptation to Diverse Environments within the Host
    Article Snippet: For RNA stabilization, a 2:1 (vol/vol) ratio of RNA protect (Qiagen) was added to 250 μl of bacterial sample and spun down (Thermo Scientific Legend Micro 21, room temperature, 5 min, 9,600 × g ). .. Subsequent RNA isolation was performed with an RNeasy minikit (Qiagen), followed by DNase treatment of RNA with a Turbo DNA-free kit (Invitrogen).

    Article Title: Establishment of an Analytical System for the Human Fecal Microbiota, Based on Reverse Transcription-Quantitative PCR Targeting of Multicopy rRNA Molecules ▿Establishment of an Analytical System for the Human Fecal Microbiota, Based on Reverse Transcription-Quantitative PCR Targeting of Multicopy rRNA Molecules ▿ †
    Article Snippet: Paragraph title: Total RNA isolation. ... For RNA stabilization, fresh cultures of each bacterial (50 μl) or fecal (200 μl) homogenate were added to 2 volumes of RNAprotect bacterial reagent (Qiagen GmbH, Hilden, Germany) and then incubated for 5 min at room temperature.

    Article Title: A 3-day EGCG-supplementation reduces interstitial lactate concentration in skeletal muscle of overweight subjects
    Article Snippet: .. Total RNA was isolated, using the total RNA stabilization and purification kit for human samples Qiagen (Qiagen, Hombrechtikon, Switzerland). .. Gene expression of HSL, ATGL, CPT-1, ACC-1, FAT/CD36 and leptin was measured by the Taqman multiplex method using the ABI 7900 quantitative real-time RT-PCR instrument (Applied Biosystems, Rotkreuz, Switzerland) as described by Heim .

    Article Title: Quorum Sensing Modulates the Epibiotic-Parasitic Relationship Between Actinomyces odontolyticus and Its Saccharibacteria epibiont, a Nanosynbacter lyticus Strain, TM7x
    Article Snippet: .. Prior to RNA isolation, cell pellets were treated for RNA stabilization with RNA protect Cell Reagent (Qiagen, Cat. No./ID 76526). .. Total RNA was isolated using RNeasy Protect Bacteria Mini Kit (Qiagen, Cat. No./ID: 74524) following the manufacturer’s protocol.

    Article Title: Oligodendrocytes control potassium accumulation in white matter and seizure susceptibility
    Article Snippet: EGFP+ cells were isolated using the MoFlo MLS high-speed cell sorter (Beckman Coulter, Brea, CA) at the Johns Hopkins School of Public Health FACS core. .. RNA stabilization, genomic DNA elimination, and reverse transcription were performed using the FastLane Cell cDNA Kit (Qiagen, Hilden, Germany), following the manufacturer’s instructions.

    Article Title: Heat Resistance and Salt Hypersensitivity in Lactococcus lactis Due to Spontaneous Mutation of llmg_1816 (gdpP) Induced by High-Temperature Growth
    Article Snippet: Paragraph title: RNA isolation, cDNA synthesis, and qPCR. ... Early-log-phase (OD600 = 0.2) cultures were first subjected to RNA stabilization with RNA protect (Qiagen, Hilden, Germany) and harvested by centrifugation (16,000 × g , 5 min), and RNA was extracted using the RNeasy minikit (Qiagen, Hilden, Germany).

    Article Title: The Role of Flagella in Clostridium difficile Pathogenesis: Comparison between a Non-Epidemic and an Epidemic Strain
    Article Snippet: RNA stabilization was achieved by using RNAprotect Bacteria Reagent and an RNeasy Mini Kit (Qiagen) as described by the manufacturer's instructions. .. RNA isolation was then carried out using the FastRNA pro Blue extraction Kit (MP Biosciences), followed by phenol-chloroform-isoamyl alcohol (25∶24∶1) treatment and precipitated with ethanol according to the manufacturer's instructions.

    Size-exclusion Chromatography:

    Article Title: Leapfrog diagnostics: Demonstration of a broad spectrum pathogen identification platform in a resource-limited setting
    Article Snippet: Reverse transcription and real time PCR (RT-PCR) In order to detect influenza B RNA in testing RNA stabilization on FTA 1 mm processed disks were placed in a 0.5 mL PCR tube, and subjected to one step reverse transcription and PCR (RT-PCR) using Qiagen OneStep RT-PCR Kit (Qiagen, Valencia, CA) according to the manufacturer’s instructions. .. The RT-PCR reaction was run in 25 μl total volume using previously published BMA-F1 and BMA-R1 primers for detection of influenza B [ ] and the following thermal cycling protocol: 50°C for 30 min; 95°C for 15 min.; 40 cycles of 94°C for 30 sec., 54°C 30 sec., 72°C 30 sec.; 72°C 10 min.

    Mouse Assay:

    Article Title: Oligodendrocytes control potassium accumulation in white matter and seizure susceptibility
    Article Snippet: Fluorescence-activated cell sorting and RT-qPCR P70 mice were deeply anesthetized with pentobarbital and transcardially perfused with ice-cold Hank's Balanced Salt Solution without Ca2+ or Mg2+ (HBSS; Gibco Laboratories, Gaithersburg, MD). .. RNA stabilization, genomic DNA elimination, and reverse transcription were performed using the FastLane Cell cDNA Kit (Qiagen, Hilden, Germany), following the manufacturer’s instructions.

    Polymerase Chain Reaction:

    Article Title: Involvement of the different lung compartments in the pathogenesis of pH1N1 influenza virus infection in ferrets
    Article Snippet: RNA extraction was performed on the ferret lung tissue samples with an RNeasy Mini Kit (Qiagen), using the RNA stabilization and on-column DNase digestion protocols (Qiagen). .. PCR reactions were performed in 10 µL reaction volumes in quadruplicates; 45 amplification cycles were used, and the annealing temperature was 60 °C.

    Article Title: Flexible Metabolism and Suppression of Latent Enzymes Are Important for Escherichia coli Adaptation to Diverse Environments within the Host
    Article Snippet: For RNA stabilization, a 2:1 (vol/vol) ratio of RNA protect (Qiagen) was added to 250 μl of bacterial sample and spun down (Thermo Scientific Legend Micro 21, room temperature, 5 min, 9,600 × g ). .. PCR with primers to amplify the glyceraldehyde-3-phosphate dehydrogenase housekeeping gene ( gapA ) was performed to check for contaminating DNA. cDNA synthesis was performed on DNA-free RNA samples with an iScript kit (Bio-Rad).

    Article Title: Leapfrog diagnostics: Demonstration of a broad spectrum pathogen identification platform in a resource-limited setting
    Article Snippet: .. Reverse transcription and real time PCR (RT-PCR) In order to detect influenza B RNA in testing RNA stabilization on FTA 1 mm processed disks were placed in a 0.5 mL PCR tube, and subjected to one step reverse transcription and PCR (RT-PCR) using Qiagen OneStep RT-PCR Kit (Qiagen, Valencia, CA) according to the manufacturer’s instructions. ..

    Article Title: Oligodendrocytes control potassium accumulation in white matter and seizure susceptibility
    Article Snippet: RNA stabilization, genomic DNA elimination, and reverse transcription were performed using the FastLane Cell cDNA Kit (Qiagen, Hilden, Germany), following the manufacturer’s instructions. .. Real time qPCR was performed using the QuantiTect SYBR Green PCR Kit (Qiagen), following the manufacturer’s instructions, and using a StepOnePlus Real-Time PCR System (Applied Biosystems, Foster City, CA).

    Purification:

    Article Title: A 3-day EGCG-supplementation reduces interstitial lactate concentration in skeletal muscle of overweight subjects
    Article Snippet: .. Total RNA was isolated, using the total RNA stabilization and purification kit for human samples Qiagen (Qiagen, Hombrechtikon, Switzerland). .. Gene expression of HSL, ATGL, CPT-1, ACC-1, FAT/CD36 and leptin was measured by the Taqman multiplex method using the ABI 7900 quantitative real-time RT-PCR instrument (Applied Biosystems, Rotkreuz, Switzerland) as described by Heim .

    Article Title: Quorum Sensing Modulates the Epibiotic-Parasitic Relationship Between Actinomyces odontolyticus and Its Saccharibacteria epibiont, a Nanosynbacter lyticus Strain, TM7x
    Article Snippet: Prior to RNA isolation, cell pellets were treated for RNA stabilization with RNA protect Cell Reagent (Qiagen, Cat. No./ID 76526). .. RNA Clean Concentrator Kit (Zymo Research RNA Clean Concentrator Cat. No./ID R1015) was applied for terminal purification of the RNA.

    Article Title: The Role of Flagella in Clostridium difficile Pathogenesis: Comparison between a Non-Epidemic and an Epidemic Strain
    Article Snippet: RNA stabilization was achieved by using RNAprotect Bacteria Reagent and an RNeasy Mini Kit (Qiagen) as described by the manufacturer's instructions. .. The RNA was purified using the RNeasy spin column purification kit (QIAGEN) according to the manufacturer's instructions.

    RNA Extraction:

    Article Title: Involvement of the different lung compartments in the pathogenesis of pH1N1 influenza virus infection in ferrets
    Article Snippet: .. RNA extraction was performed on the ferret lung tissue samples with an RNeasy Mini Kit (Qiagen), using the RNA stabilization and on-column DNase digestion protocols (Qiagen). .. Reverse transcription was performed using an ImProm-II reverse transcription system (Promega, Madison, WI, USA), at 0.5 µg RNA.

    Article Title: The Role of Flagella in Clostridium difficile Pathogenesis: Comparison between a Non-Epidemic and an Epidemic Strain
    Article Snippet: Paragraph title: RNA extraction and reverse transcription ... RNA stabilization was achieved by using RNAprotect Bacteria Reagent and an RNeasy Mini Kit (Qiagen) as described by the manufacturer's instructions.

    Electrophoresis:

    Article Title: Heat Resistance and Salt Hypersensitivity in Lactococcus lactis Due to Spontaneous Mutation of llmg_1816 (gdpP) Induced by High-Temperature Growth
    Article Snippet: Early-log-phase (OD600 = 0.2) cultures were first subjected to RNA stabilization with RNA protect (Qiagen, Hilden, Germany) and harvested by centrifugation (16,000 × g , 5 min), and RNA was extracted using the RNeasy minikit (Qiagen, Hilden, Germany). .. The quality and integrity of RNA was confirmed by electrophoresis. cDNA synthesis reactions were standardized to contain 0.5 μg of RNA (including no reverse transcriptase controls to ensure the absence of DNA contamination) and were then reverse transcribed (where necessary) using the Superscript III first-strand synthesis supermix (Invitrogen, Carlsbad, CA).

    Multiplex Assay:

    Article Title: A 3-day EGCG-supplementation reduces interstitial lactate concentration in skeletal muscle of overweight subjects
    Article Snippet: Total RNA was isolated, using the total RNA stabilization and purification kit for human samples Qiagen (Qiagen, Hombrechtikon, Switzerland). .. Gene expression of HSL, ATGL, CPT-1, ACC-1, FAT/CD36 and leptin was measured by the Taqman multiplex method using the ABI 7900 quantitative real-time RT-PCR instrument (Applied Biosystems, Rotkreuz, Switzerland) as described by Heim .

    Homogenization:

    Article Title: Analysis of gene expression in mouse brain regions after exposure to 1.9 GHz radiofrequency fields
    Article Snippet: .. For tissue disruption and RNA stabilization, each sample was treated consecutively, as follows: a 350 μ l aliquot of RLT buffer (Qiagen Inc.) containing 1% β-mercaptoethanol was added directly onto the frozen tissue sample and then immediately sonicated (Micro Ultrasonic Cell Disruptor, Kontes Inc.) for 5 s in the microtube with a sterilized probe treated with RNase-Zap, followed immediately by homogenization in the microtube for 30 s using a handheld homogenizer (Pellet Pestle Motor, Kontes Inc.) with disposable RNase/DNase-free pestles (Kimble-Chase Inc.). ..

    Laser Capture Microdissection:

    Article Title: Involvement of the different lung compartments in the pathogenesis of pH1N1 influenza virus infection in ferrets
    Article Snippet: Gene expression profiles Total RNA was isolated from LCM tissues, using the miRNeasy FFPE Kit (no. 217504, Qiagen), as described in the above section. .. RNA extraction was performed on the ferret lung tissue samples with an RNeasy Mini Kit (Qiagen), using the RNA stabilization and on-column DNase digestion protocols (Qiagen).

    Spectrophotometry:

    Article Title: Analysis of gene expression in mouse brain regions after exposure to 1.9 GHz radiofrequency fields
    Article Snippet: For tissue disruption and RNA stabilization, each sample was treated consecutively, as follows: a 350 μ l aliquot of RLT buffer (Qiagen Inc.) containing 1% β-mercaptoethanol was added directly onto the frozen tissue sample and then immediately sonicated (Micro Ultrasonic Cell Disruptor, Kontes Inc.) for 5 s in the microtube with a sterilized probe treated with RNase-Zap, followed immediately by homogenization in the microtube for 30 s using a handheld homogenizer (Pellet Pestle Motor, Kontes Inc.) with disposable RNase/DNase-free pestles (Kimble-Chase Inc.). .. Following elution from the RNeasy MinElute columns (Qiagen Inc.) into RNase-free water, the RNA concentration was evaluated using a NanoDrop 2000 spectrophotometer (ThermoScientific Inc.) and RNA integrity was confirmed using an Agilent 2100 Bioanalyzer with RNA 6000 PicoChip kits (Agilent Technologies Inc.).

    Concentration Assay:

    Article Title: Analysis of gene expression in mouse brain regions after exposure to 1.9 GHz radiofrequency fields
    Article Snippet: For tissue disruption and RNA stabilization, each sample was treated consecutively, as follows: a 350 μ l aliquot of RLT buffer (Qiagen Inc.) containing 1% β-mercaptoethanol was added directly onto the frozen tissue sample and then immediately sonicated (Micro Ultrasonic Cell Disruptor, Kontes Inc.) for 5 s in the microtube with a sterilized probe treated with RNase-Zap, followed immediately by homogenization in the microtube for 30 s using a handheld homogenizer (Pellet Pestle Motor, Kontes Inc.) with disposable RNase/DNase-free pestles (Kimble-Chase Inc.). .. Following elution from the RNeasy MinElute columns (Qiagen Inc.) into RNase-free water, the RNA concentration was evaluated using a NanoDrop 2000 spectrophotometer (ThermoScientific Inc.) and RNA integrity was confirmed using an Agilent 2100 Bioanalyzer with RNA 6000 PicoChip kits (Agilent Technologies Inc.).

    FACS:

    Article Title: Oligodendrocytes control potassium accumulation in white matter and seizure susceptibility
    Article Snippet: Paragraph title: Fluorescence-activated cell sorting and RT-qPCR ... RNA stabilization, genomic DNA elimination, and reverse transcription were performed using the FastLane Cell cDNA Kit (Qiagen, Hilden, Germany), following the manufacturer’s instructions.

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    Qiagen rna stabilization
    Proposed mechanisms of how active tristetraprolin (TTP) confers protection against the development of cigarette smoke (CS)‐induced experimental COPD. CS exposure increases the messenger ribonucleic acid <t>(mRNA)</t> expression of inflammatory mediators resulting in pulmonary inflammation. Ongoing inflammation drives the development of airway remodelling and emphysema. These features collectively result in lung function decline. Active TTP degrades the mRNA of inflammatory mediators, preventing the development of pulmonary inflammation, airway remodelling, emphysema and consequent lung function decline. TTP, tristetraprolin; mRNA, messenger ribonucleic acid; COPD, chronic obstructive pulmonary disease.
    Rna Stabilization, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rna stabilization/product/Qiagen
    Average 99 stars, based on 4 article reviews
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    rna stabilization - by Bioz Stars, 2020-04
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    Proposed mechanisms of how active tristetraprolin (TTP) confers protection against the development of cigarette smoke (CS)‐induced experimental COPD. CS exposure increases the messenger ribonucleic acid (mRNA) expression of inflammatory mediators resulting in pulmonary inflammation. Ongoing inflammation drives the development of airway remodelling and emphysema. These features collectively result in lung function decline. Active TTP degrades the mRNA of inflammatory mediators, preventing the development of pulmonary inflammation, airway remodelling, emphysema and consequent lung function decline. TTP, tristetraprolin; mRNA, messenger ribonucleic acid; COPD, chronic obstructive pulmonary disease.

    Journal: Clinical & Translational Immunology

    Article Title: Enhancing tristetraprolin activity reduces the severity of cigarette smoke‐induced experimental chronic obstructive pulmonary disease

    doi: 10.1002/cti2.1084

    Figure Lengend Snippet: Proposed mechanisms of how active tristetraprolin (TTP) confers protection against the development of cigarette smoke (CS)‐induced experimental COPD. CS exposure increases the messenger ribonucleic acid (mRNA) expression of inflammatory mediators resulting in pulmonary inflammation. Ongoing inflammation drives the development of airway remodelling and emphysema. These features collectively result in lung function decline. Active TTP degrades the mRNA of inflammatory mediators, preventing the development of pulmonary inflammation, airway remodelling, emphysema and consequent lung function decline. TTP, tristetraprolin; mRNA, messenger ribonucleic acid; COPD, chronic obstructive pulmonary disease.

    Article Snippet: mRNA expression Whole lungs were collected and stored in RNA Stabilization Reagent, RNAlater (Qiagen, Chadstone Centre, Vic, Australia).

    Techniques: Expressing