rna quantity  (Thermo Fisher)


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    Structured Review

    Thermo Fisher rna quantity
    Rna Quantity, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rna quantity/product/Thermo Fisher
    Average 99 stars, based on 38 article reviews
    Price from $9.99 to $1999.99
    rna quantity - by Bioz Stars, 2020-08
    99/100 stars

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    RNA Sequencing Assay:

    Article Title: Transcriptomic Analyses Revealed Systemic Alterations in Gene Expression in Circulation and Tumor Microenvironment of Colorectal Cancer Patients
    Article Snippet: .. Library Preparation and RNA sequencing The RNA was quantified using Qubit instrument (Invitrogen, Carlsbad, CA, USA) and RNA BR assay kit (Invitrogen). .. 100 ng of RNA was used as input for library preparation using TruSeq RNA Access Library preparation kit (Illumina, San Diego, CA, USA) as per the manufacturer’s instructions.

    Synthesized:

    Article Title: Macrophage Coordination of the Interferon Lambda Immune Response
    Article Snippet: .. Digital Droplet PCR (ddPCR) Immune cell RNA was quantified using the Qubit fluorometer and RNA BR assay kit (Thermo Fisher), and cDNA was synthesized from ≥10 ng of RNA per sample using qScript cDNA supermix (Quantabio). cDNA was combined with ddPCR supermix and droplet generation oil for probes (Bio-Rad), and droplets were generated using the Bio-Rad QX200 Droplet Generator. .. PCR was performed using IFNLR1 and GAPDH probes according to the manufacturer's instructions, and droplet fluorescence was analyzed using the Bio-Rad QX200 Droplet Reader.

    Spectrophotometry:

    Article Title: Comparison of the myometrial transcriptome from singleton and twin pregnancies by RNA-Seq
    Article Snippet: .. The quantity and purity of RNA was determined using a Qubit RNA BR Assay Kit and fluorometer (Invitrogen, UK) and NanoDrop® ND-1000 spectrophotometer (Thermo Scientific, UK). ..

    Generated:

    Article Title: Macrophage Coordination of the Interferon Lambda Immune Response
    Article Snippet: .. Digital Droplet PCR (ddPCR) Immune cell RNA was quantified using the Qubit fluorometer and RNA BR assay kit (Thermo Fisher), and cDNA was synthesized from ≥10 ng of RNA per sample using qScript cDNA supermix (Quantabio). cDNA was combined with ddPCR supermix and droplet generation oil for probes (Bio-Rad), and droplets were generated using the Bio-Rad QX200 Droplet Generator. .. PCR was performed using IFNLR1 and GAPDH probes according to the manufacturer's instructions, and droplet fluorescence was analyzed using the Bio-Rad QX200 Droplet Reader.

    other:

    Article Title: A novel method for RNA extraction from FFPE samples reveals significant differences in biomarker expression between orthotopic and subcutaneous pancreatic cancer patient-derived xenografts
    Article Snippet: For Qubit runs, the instrument was calibrated using Qubit® RNA Broad-Range assay kit by Life Technologies.

    Polymerase Chain Reaction:

    Article Title: Macrophage Coordination of the Interferon Lambda Immune Response
    Article Snippet: .. Digital Droplet PCR (ddPCR) Immune cell RNA was quantified using the Qubit fluorometer and RNA BR assay kit (Thermo Fisher), and cDNA was synthesized from ≥10 ng of RNA per sample using qScript cDNA supermix (Quantabio). cDNA was combined with ddPCR supermix and droplet generation oil for probes (Bio-Rad), and droplets were generated using the Bio-Rad QX200 Droplet Generator. .. PCR was performed using IFNLR1 and GAPDH probes according to the manufacturer's instructions, and droplet fluorescence was analyzed using the Bio-Rad QX200 Droplet Reader.

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    Thermo Fisher quantitative rt pcr analysis total rna
    Stable forced SUMO1 expression enhanced the colony formation, proliferation, migration, cell cycle progression, and invasion of A549 cells in vitro. ( a ) Detection of messenger <t>RNA</t> (mRNA) expression of SUMO1 in different lung cancer cell lines by quantitative real time <t>(qRT)‐PCR.</t> ( b ) Similar results were obtained through Western blot analysis. ( c ) qRT‐PCR analysis revealed that SUMO1 mRNA expression levels were increased in SUMO1 overexpressed A549 cells compared to control cells. ( d ) Similar results were obtained through Western blot analysis (passages 15 and 30). Upregulation of SUMO1 enhanced the ( e , f ) colony‐formation ability, ( g ) proliferation, ( h , i ) migration, and ( k , l ) invasion of A549 cells. ( j ) Forced expression of SUMO1 increased the number of A549 cells in the S phase of the cell cycle. * P
    Quantitative Rt Pcr Analysis Total Rna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 169 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quantitative rt pcr analysis total rna/product/Thermo Fisher
    Average 94 stars, based on 169 article reviews
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    Thermo Fisher quantitative real time polymerase chain reaction qrt pcr total rna
    Up-regulation of miR-96 and down-regulation of FOXO1 in tumor tissues and HepG2 cells. a miR-96 relative expression in tumor tissues and non-tumor tissues by <t>qRT-PCR;</t> b FOXO1 mRNA relative expression in tumor tissues and non-tumor tissues by qRT-PCR; c FOXO1 protein relative expression in tumor tissues and non-tumor tissues by Western blot; d miR-96 relative expression in L02 and HepG2 cells by qRT-PCR; e FOXO1 mRNA relative expression in L02 and HepG2 cells by qRT-PCR; f FOXO1 protein relative expression in L02 and HepG2 cells by Western blot. ** P
    Quantitative Real Time Polymerase Chain Reaction Qrt Pcr Total Rna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 424 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Thermo Fisher real time quantitative pcr qpcr total rna
    Pcf11 Expression Is Regulated by IPA (A) Schematic of mouse Pcf11 gene. Multiple PASs are indicated. EPA, internal exonic polyadenylation; IPA, intronic polyadenylation; TPA, 3′ terminal exon polyadenylation. 3′READS data of brain, heart, and testis are shown. (B) Features of intron 1 compared with other introns in the mouse genome. Each feature has ten bins, and the bin containing intron 1 of Pcf11 is highlighted in red. Distance to TSS is based on all IPA sites in PolyA_DB. (C) <t>RT-qPCR</t> analysis of IPA and TPA isoform expression in siPcf11 versus siCtrl cells. Error bars are SD on the basis of three replicates. (D) Top: schematic showing two sgRNAs used to remove a region flanking the IPA site. Bottom: validation of IPA site knockout (IPA Pcf11 -KO) by genomic DNA <t>PCR</t> using primers flanking the KO region. WT, wild-type 4T1 cells. (E) RT-qPCR analysis of Pcf11 isoform expression in WT and IPA Pcf11 -KO cells. Error bars are SD on the basis of three replicates. (F) Western blot analysis of PCF11 in WT and IPA Pcf11 -KO cells. (G) 3′UTR APA changes in IPA Pcf11 -KO cells. (H) IPA changes in IPA Pcf11 -KO cells. IPA isoforms (all combined) were compared with TPA isoforms (all combined) in analysis. (I) IPA distribution maps of activated IPA events in IPA Pcf11 -KO cells. (J) Left: schematic of PASs around the transcription start site (TSS); right: metagene analysis of PAS usage around the TSS in WT and IPA Pcf1 1 -KO cells. (K) Gene expression changes in IPA Pcf11 -KO cells for different groups of genes on the basis of IPA site frequency in PolyA_DB. ***p
    Real Time Quantitative Pcr Qpcr Total Rna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 101 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/real time quantitative pcr qpcr total rna/product/Thermo Fisher
    Average 92 stars, based on 101 article reviews
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    92
    Thermo Fisher semi quantitative rt pcr total rna
    Loss of SNRNP70 leads to alternative splicing errors Data are based on <t>RNA-seq</t> in 28 hpf embryos derived from overexpression of cytosolic hSNRNP70-eGFP in sibling and null animals using the Tg(ubi:ERT2-Gal4;UAS:hSNRNP70ΔNLS3xNES-eGFP) line. Embryos were sorted into four groups according to genotype: (1) sib/GFP − , (2) sib/GFP + , (3) null/GFP − and (4) null/GFP + . (A) Key for alternative splicing changes shown in (B-D) . (B) Graph showing the number of alternative splicing events that appear significantly different (MV.dPSI > 0.1) comparing null/GFP − to sib/GFP − embryos. n = 3 biological samples per genotype. (C) Graph showing the number of alternative splicing events that are significantly (MV.dPSI > 0.1) restored (shown in red) or not (gray) following overexpression of cytosolic hSNRNP70-eGFP in null embryos. These data are based on comparison between sib/GFP − vs null/GFP − and then between null/GFP − vs null/GFP + . (D) Graph of the 93 rescued alternative splicing events from (C) depicting the effect SNRNP70 loss had on these events prior to the rescue. These data are based on comparison between sib/GFP − vs null/GFP − and then between null/GFP − vs null/GFP + . (E-F) Schematic plots for a representative example of CE ( rapgef5a exon7 ) and IR ( syt8 intron 4 ), respectively, in the four experimental groups. (G-H) Semiquantitative <t>RT-PCR</t> validation of exon skipping events ( rapgef5a exon7 and ptprnb exon16 ). The graphs show mean values ± SEM of the percentage of exon skipping over the total expression of that transcript in four experimental groups. **** P
    Semi Quantitative Rt Pcr Total Rna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 181 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 92 stars, based on 181 article reviews
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    Stable forced SUMO1 expression enhanced the colony formation, proliferation, migration, cell cycle progression, and invasion of A549 cells in vitro. ( a ) Detection of messenger RNA (mRNA) expression of SUMO1 in different lung cancer cell lines by quantitative real time (qRT)‐PCR. ( b ) Similar results were obtained through Western blot analysis. ( c ) qRT‐PCR analysis revealed that SUMO1 mRNA expression levels were increased in SUMO1 overexpressed A549 cells compared to control cells. ( d ) Similar results were obtained through Western blot analysis (passages 15 and 30). Upregulation of SUMO1 enhanced the ( e , f ) colony‐formation ability, ( g ) proliferation, ( h , i ) migration, and ( k , l ) invasion of A549 cells. ( j ) Forced expression of SUMO1 increased the number of A549 cells in the S phase of the cell cycle. * P

    Journal: Thoracic Cancer

    Article Title: SUMO1 promotes the proliferation and invasion of non‐small cell lung cancer cells by regulating NF‐κB

    doi: 10.1111/1759-7714.12895

    Figure Lengend Snippet: Stable forced SUMO1 expression enhanced the colony formation, proliferation, migration, cell cycle progression, and invasion of A549 cells in vitro. ( a ) Detection of messenger RNA (mRNA) expression of SUMO1 in different lung cancer cell lines by quantitative real time (qRT)‐PCR. ( b ) Similar results were obtained through Western blot analysis. ( c ) qRT‐PCR analysis revealed that SUMO1 mRNA expression levels were increased in SUMO1 overexpressed A549 cells compared to control cells. ( d ) Similar results were obtained through Western blot analysis (passages 15 and 30). Upregulation of SUMO1 enhanced the ( e , f ) colony‐formation ability, ( g ) proliferation, ( h , i ) migration, and ( k , l ) invasion of A549 cells. ( j ) Forced expression of SUMO1 increased the number of A549 cells in the S phase of the cell cycle. * P

    Article Snippet: Quantitative RT‐PCR analysis Total RNA was isolated using the TRIzol reagent according to the manufacturer's protocol (Thermo Fisher Scientific).

    Techniques: Expressing, Migration, In Vitro, Quantitative RT-PCR, Western Blot

    Downregulation of SUMO1 suppresses the proliferation, cell cycle progression, colony formation, migration, and invasion of Calu‐1 cells in vitro. ( a ) Quantitative real time‐PCR analysis revealed that the messenger RNA (mRNA) expression levels of SUMO1 in short hairpin RNA (shRNA)‐SUMO1‐1 Calu‐1 cells were significantly downregulated. ( b ) Similar results were obtained through Western blot analysis. ( c ) Downregulation of SUMO1 expression significantly inhibited the ( c ) proliferation, ( e , f ) colony‐formation ability, ( g , h ) migration, and ( i , j ) invasion of Calu‐1 cells. ( d ) Downregulation of SUMO1 reduced the number of Calu‐1 cells in the S phase of the cell cycle.

    Journal: Thoracic Cancer

    Article Title: SUMO1 promotes the proliferation and invasion of non‐small cell lung cancer cells by regulating NF‐κB

    doi: 10.1111/1759-7714.12895

    Figure Lengend Snippet: Downregulation of SUMO1 suppresses the proliferation, cell cycle progression, colony formation, migration, and invasion of Calu‐1 cells in vitro. ( a ) Quantitative real time‐PCR analysis revealed that the messenger RNA (mRNA) expression levels of SUMO1 in short hairpin RNA (shRNA)‐SUMO1‐1 Calu‐1 cells were significantly downregulated. ( b ) Similar results were obtained through Western blot analysis. ( c ) Downregulation of SUMO1 expression significantly inhibited the ( c ) proliferation, ( e , f ) colony‐formation ability, ( g , h ) migration, and ( i , j ) invasion of Calu‐1 cells. ( d ) Downregulation of SUMO1 reduced the number of Calu‐1 cells in the S phase of the cell cycle.

    Article Snippet: Quantitative RT‐PCR analysis Total RNA was isolated using the TRIzol reagent according to the manufacturer's protocol (Thermo Fisher Scientific).

    Techniques: Migration, In Vitro, Real-time Polymerase Chain Reaction, Expressing, shRNA, Western Blot

    Up-regulation of miR-96 and down-regulation of FOXO1 in tumor tissues and HepG2 cells. a miR-96 relative expression in tumor tissues and non-tumor tissues by qRT-PCR; b FOXO1 mRNA relative expression in tumor tissues and non-tumor tissues by qRT-PCR; c FOXO1 protein relative expression in tumor tissues and non-tumor tissues by Western blot; d miR-96 relative expression in L02 and HepG2 cells by qRT-PCR; e FOXO1 mRNA relative expression in L02 and HepG2 cells by qRT-PCR; f FOXO1 protein relative expression in L02 and HepG2 cells by Western blot. ** P

    Journal: Cancer Cell International

    Article Title: miR-96 exerts carcinogenic effect by activating AKT/GSK-3β/β-catenin signaling pathway through targeting inhibition of FOXO1 in hepatocellular carcinoma

    doi: 10.1186/s12935-019-0756-7

    Figure Lengend Snippet: Up-regulation of miR-96 and down-regulation of FOXO1 in tumor tissues and HepG2 cells. a miR-96 relative expression in tumor tissues and non-tumor tissues by qRT-PCR; b FOXO1 mRNA relative expression in tumor tissues and non-tumor tissues by qRT-PCR; c FOXO1 protein relative expression in tumor tissues and non-tumor tissues by Western blot; d miR-96 relative expression in L02 and HepG2 cells by qRT-PCR; e FOXO1 mRNA relative expression in L02 and HepG2 cells by qRT-PCR; f FOXO1 protein relative expression in L02 and HepG2 cells by Western blot. ** P

    Article Snippet: Quantitative real time polymerase chain reaction (qRT-PCR) Total RNA in tumor tissues and cells was obtained by Trizol kit (Thermo Fisher Scientific, Waltham, MA, USA) according to the the instruction manual.

    Techniques: Expressing, Quantitative RT-PCR, Western Blot

    FOXO1 was directly suppressed by miR-96. a Prediction of binding sites for FOXO1 and miR-96 by Target Scan; b Luciferase reporter assay; c FOXO1 mRNA expression by qRT-PCR; d FOXO1 protein expression by Western blot. ## P

    Journal: Cancer Cell International

    Article Title: miR-96 exerts carcinogenic effect by activating AKT/GSK-3β/β-catenin signaling pathway through targeting inhibition of FOXO1 in hepatocellular carcinoma

    doi: 10.1186/s12935-019-0756-7

    Figure Lengend Snippet: FOXO1 was directly suppressed by miR-96. a Prediction of binding sites for FOXO1 and miR-96 by Target Scan; b Luciferase reporter assay; c FOXO1 mRNA expression by qRT-PCR; d FOXO1 protein expression by Western blot. ## P

    Article Snippet: Quantitative real time polymerase chain reaction (qRT-PCR) Total RNA in tumor tissues and cells was obtained by Trizol kit (Thermo Fisher Scientific, Waltham, MA, USA) according to the the instruction manual.

    Techniques: Binding Assay, Luciferase, Reporter Assay, Expressing, Quantitative RT-PCR, Western Blot

    Pcf11 Expression Is Regulated by IPA (A) Schematic of mouse Pcf11 gene. Multiple PASs are indicated. EPA, internal exonic polyadenylation; IPA, intronic polyadenylation; TPA, 3′ terminal exon polyadenylation. 3′READS data of brain, heart, and testis are shown. (B) Features of intron 1 compared with other introns in the mouse genome. Each feature has ten bins, and the bin containing intron 1 of Pcf11 is highlighted in red. Distance to TSS is based on all IPA sites in PolyA_DB. (C) RT-qPCR analysis of IPA and TPA isoform expression in siPcf11 versus siCtrl cells. Error bars are SD on the basis of three replicates. (D) Top: schematic showing two sgRNAs used to remove a region flanking the IPA site. Bottom: validation of IPA site knockout (IPA Pcf11 -KO) by genomic DNA PCR using primers flanking the KO region. WT, wild-type 4T1 cells. (E) RT-qPCR analysis of Pcf11 isoform expression in WT and IPA Pcf11 -KO cells. Error bars are SD on the basis of three replicates. (F) Western blot analysis of PCF11 in WT and IPA Pcf11 -KO cells. (G) 3′UTR APA changes in IPA Pcf11 -KO cells. (H) IPA changes in IPA Pcf11 -KO cells. IPA isoforms (all combined) were compared with TPA isoforms (all combined) in analysis. (I) IPA distribution maps of activated IPA events in IPA Pcf11 -KO cells. (J) Left: schematic of PASs around the transcription start site (TSS); right: metagene analysis of PAS usage around the TSS in WT and IPA Pcf1 1 -KO cells. (K) Gene expression changes in IPA Pcf11 -KO cells for different groups of genes on the basis of IPA site frequency in PolyA_DB. ***p

    Journal: Cell reports

    Article Title: Regulation of Intronic Polyadenylation by PCF11 Impacts mRNA Expression of Long Genes

    doi: 10.1016/j.celrep.2019.02.049

    Figure Lengend Snippet: Pcf11 Expression Is Regulated by IPA (A) Schematic of mouse Pcf11 gene. Multiple PASs are indicated. EPA, internal exonic polyadenylation; IPA, intronic polyadenylation; TPA, 3′ terminal exon polyadenylation. 3′READS data of brain, heart, and testis are shown. (B) Features of intron 1 compared with other introns in the mouse genome. Each feature has ten bins, and the bin containing intron 1 of Pcf11 is highlighted in red. Distance to TSS is based on all IPA sites in PolyA_DB. (C) RT-qPCR analysis of IPA and TPA isoform expression in siPcf11 versus siCtrl cells. Error bars are SD on the basis of three replicates. (D) Top: schematic showing two sgRNAs used to remove a region flanking the IPA site. Bottom: validation of IPA site knockout (IPA Pcf11 -KO) by genomic DNA PCR using primers flanking the KO region. WT, wild-type 4T1 cells. (E) RT-qPCR analysis of Pcf11 isoform expression in WT and IPA Pcf11 -KO cells. Error bars are SD on the basis of three replicates. (F) Western blot analysis of PCF11 in WT and IPA Pcf11 -KO cells. (G) 3′UTR APA changes in IPA Pcf11 -KO cells. (H) IPA changes in IPA Pcf11 -KO cells. IPA isoforms (all combined) were compared with TPA isoforms (all combined) in analysis. (I) IPA distribution maps of activated IPA events in IPA Pcf11 -KO cells. (J) Left: schematic of PASs around the transcription start site (TSS); right: metagene analysis of PAS usage around the TSS in WT and IPA Pcf1 1 -KO cells. (K) Gene expression changes in IPA Pcf11 -KO cells for different groups of genes on the basis of IPA site frequency in PolyA_DB. ***p

    Article Snippet: Real-time quantitative PCR (qPCR) Total RNA was extracted using TRIzol (Thermo Fisher) and treated with Turbo DNase (Thermo Fisher). cDNA was synthesized using oligo(dT) and M-MLV reverse transcriptase with 1.5–2 μg of RNA. cDNA was then mixed with real-time PCR primers and Luna qPCR master mix (NEB).

    Techniques: Expressing, Indirect Immunoperoxidase Assay, Quantitative RT-PCR, Knock-Out, Polymerase Chain Reaction, Western Blot

    Loss of SNRNP70 leads to alternative splicing errors Data are based on RNA-seq in 28 hpf embryos derived from overexpression of cytosolic hSNRNP70-eGFP in sibling and null animals using the Tg(ubi:ERT2-Gal4;UAS:hSNRNP70ΔNLS3xNES-eGFP) line. Embryos were sorted into four groups according to genotype: (1) sib/GFP − , (2) sib/GFP + , (3) null/GFP − and (4) null/GFP + . (A) Key for alternative splicing changes shown in (B-D) . (B) Graph showing the number of alternative splicing events that appear significantly different (MV.dPSI > 0.1) comparing null/GFP − to sib/GFP − embryos. n = 3 biological samples per genotype. (C) Graph showing the number of alternative splicing events that are significantly (MV.dPSI > 0.1) restored (shown in red) or not (gray) following overexpression of cytosolic hSNRNP70-eGFP in null embryos. These data are based on comparison between sib/GFP − vs null/GFP − and then between null/GFP − vs null/GFP + . (D) Graph of the 93 rescued alternative splicing events from (C) depicting the effect SNRNP70 loss had on these events prior to the rescue. These data are based on comparison between sib/GFP − vs null/GFP − and then between null/GFP − vs null/GFP + . (E-F) Schematic plots for a representative example of CE ( rapgef5a exon7 ) and IR ( syt8 intron 4 ), respectively, in the four experimental groups. (G-H) Semiquantitative RT-PCR validation of exon skipping events ( rapgef5a exon7 and ptprnb exon16 ). The graphs show mean values ± SEM of the percentage of exon skipping over the total expression of that transcript in four experimental groups. **** P

    Journal: bioRxiv

    Article Title: Cytoplasmic pool of spliceosome protein SNRNP70 regulates the axonal transcriptome and development of motor connectivity

    doi: 10.1101/2020.05.25.097444

    Figure Lengend Snippet: Loss of SNRNP70 leads to alternative splicing errors Data are based on RNA-seq in 28 hpf embryos derived from overexpression of cytosolic hSNRNP70-eGFP in sibling and null animals using the Tg(ubi:ERT2-Gal4;UAS:hSNRNP70ΔNLS3xNES-eGFP) line. Embryos were sorted into four groups according to genotype: (1) sib/GFP − , (2) sib/GFP + , (3) null/GFP − and (4) null/GFP + . (A) Key for alternative splicing changes shown in (B-D) . (B) Graph showing the number of alternative splicing events that appear significantly different (MV.dPSI > 0.1) comparing null/GFP − to sib/GFP − embryos. n = 3 biological samples per genotype. (C) Graph showing the number of alternative splicing events that are significantly (MV.dPSI > 0.1) restored (shown in red) or not (gray) following overexpression of cytosolic hSNRNP70-eGFP in null embryos. These data are based on comparison between sib/GFP − vs null/GFP − and then between null/GFP − vs null/GFP + . (D) Graph of the 93 rescued alternative splicing events from (C) depicting the effect SNRNP70 loss had on these events prior to the rescue. These data are based on comparison between sib/GFP − vs null/GFP − and then between null/GFP − vs null/GFP + . (E-F) Schematic plots for a representative example of CE ( rapgef5a exon7 ) and IR ( syt8 intron 4 ), respectively, in the four experimental groups. (G-H) Semiquantitative RT-PCR validation of exon skipping events ( rapgef5a exon7 and ptprnb exon16 ). The graphs show mean values ± SEM of the percentage of exon skipping over the total expression of that transcript in four experimental groups. **** P

    Article Snippet: Quantitative and semi-quantitative RT-PCR Total RNA was extracted as described above for RNA sequencing. cDNA was synthesized using a First strand cDNA synthesis kit (Thermo Fisher Scientific-K1612) after treating the RNA isolated with DNaseI (QIAGEN-79254).

    Techniques: RNA Sequencing Assay, Derivative Assay, Over Expression, Reverse Transcription Polymerase Chain Reaction, Expressing