rna plant mini kit  (Qiagen)

 
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    Name:
    RNeasy Plant Mini Kit
    Description:
    For purification of total RNA from plants and fungi Kit contents Qiagen RNeasy Plant Mini Kit 20 preps 10 to 100mg Sample 30 to 100L Elution Volume Plant Sample Total RNA Purification Spin Column Format Silica Technology Ideal for Northern Dot and Slot Blotting End point RT PCR Quantitative Real time RT PCR Array Analysis Includes 20 RNeasy Mini Spin Columns 20 QIAshredder Mini Spin Columns Collection Tubes 1 5mL and 2mL RNase free Reagents and Buffers Benefits High quality total RNA in 30 minutes No phenol chloroform extraction No CsCl gradients no LiCl or ethanol precipitation Excellent recovery of RNA Ready to use RNA for any downstream applicatio
    Catalog Number:
    74903
    Price:
    155
    Category:
    RNeasy Plant Mini Kit
    Buy from Supplier


    Structured Review

    Qiagen rna plant mini kit
    RNeasy Plant Mini Kit
    For purification of total RNA from plants and fungi Kit contents Qiagen RNeasy Plant Mini Kit 20 preps 10 to 100mg Sample 30 to 100L Elution Volume Plant Sample Total RNA Purification Spin Column Format Silica Technology Ideal for Northern Dot and Slot Blotting End point RT PCR Quantitative Real time RT PCR Array Analysis Includes 20 RNeasy Mini Spin Columns 20 QIAshredder Mini Spin Columns Collection Tubes 1 5mL and 2mL RNase free Reagents and Buffers Benefits High quality total RNA in 30 minutes No phenol chloroform extraction No CsCl gradients no LiCl or ethanol precipitation Excellent recovery of RNA Ready to use RNA for any downstream applicatio
    https://www.bioz.com/result/rna plant mini kit/product/Qiagen
    Average 99 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    rna plant mini kit - by Bioz Stars, 2020-09
    99/100 stars

    Images

    1) Product Images from "Efficient and High-Quality RNA Isolation from Metabolite-Rich Tissues of Stevia rebaudiana, an Important Commercial Crop"

    Article Title: Efficient and High-Quality RNA Isolation from Metabolite-Rich Tissues of Stevia rebaudiana, an Important Commercial Crop

    Journal: Tropical Life Sciences Research

    doi: 10.21315/tlsr2019.30.1.9

    Agarose gel electrophoresis and the respective electropherograms of the total RNA samples extracted from (a) leaves, and (b) stems, of Stevia rebaudiana . Two distinct rRNA bands and peaks were clearly observed, for each sample, in both the gel and electropherograms respectively, signifying high RNA quality and minimal degradation. LT1-LT3: Replicates of RNA extracts from leaf tissues (400–600 ng); ST1-ST3: Replicates of RNA extracts from stem tissues (300–400 ng).
    Figure Legend Snippet: Agarose gel electrophoresis and the respective electropherograms of the total RNA samples extracted from (a) leaves, and (b) stems, of Stevia rebaudiana . Two distinct rRNA bands and peaks were clearly observed, for each sample, in both the gel and electropherograms respectively, signifying high RNA quality and minimal degradation. LT1-LT3: Replicates of RNA extracts from leaf tissues (400–600 ng); ST1-ST3: Replicates of RNA extracts from stem tissues (300–400 ng).

    Techniques Used: Agarose Gel Electrophoresis

    Related Articles

    RNA Extraction:

    Article Title: Biosynthesis of Lipoic Acid in Arabidopsis: Cloning and Characterization of the cDNA for Lipoic Acid Synthase 1
    Article Snippet: .. Total RNAs used for cDNA synthesis and RT-PCR were extracted from leaves, roots, and flowers of Arabidopsis using an RNA-extraction kit (RNeasy plant kit, Qiagen, Chatsworth, CA). .. RT-PCR analysis of the LIP1 gene was performed with an RT-PCR kit (SuperScript OneStep System, Life Technologies) using the following two primers: 5′-TTGGGGATACATGTACACGC-3′ and 5′-CTGAATCCCATTTCCATGCC-3′.

    Article Title: Multiplex Detection of Aspergillus fumigatus Mycoviruses
    Article Snippet: .. RNA Extraction and Reverse Transcription In order to perform multiplex PCR for mycovirus-infected A. fumigatus isolates, total fungal RNA was extracted using the RNeasy Plant Mini kit (Qiagen, Hilden, Germany) from 100 mg of grounded mycelium, quantified by using NanoDrop 2000C spectrophotometer (Thermo Fischer, Waltham, MA, USA) and cDNA was synthesized using Superscript-III first-strand synthesis system (Invitrogen, Carlsbad, CA, USA) according to the manufacturers’ protocol as follows; 5 µg of RNA, 10 mM of dNTP mix and 250 ng of random primers along with 6.5 µL of DEPC-treated water were incubated at 65 °C for 5 min and snap cooled on ice for at least 2 min. Then 4 µL of 5× first strand buffer, 0.1 M DTT, 40 U of RNasin RNase inhibitor (Promega, Madison, WI, USA) and 200 U of Superscript-III reverse transcriptase were added to the reaction after a brief centrifugation. .. The 20 µL reaction mixture was then subjected to the following cycling regime of incubation at 25 °C, 50 °C and 70 °C for 5 min, 1 h and 15 min, respectively in a DNA Engine DYAD thermocycler.

    Article Title: A highly effective and versatile technology for the isolation of RNAs from grapevines and other woody perennials for use in virus diagnostics
    Article Snippet: .. These five kits are TRIzol Reagent (Life Technologies), RNeasy Plant mini kit (Qiagen), Spectrum™ Plant Total RNA kit (Sigma), AccuPrep viral RNA extraction kit (Bioneer) and Plant/fungi total RNA kit (Norgen BioTek). .. These kits were chosen due to their advantages in certain aspects in RNA isolation.

    Article Title: Molecular Cloning and Characterization of Novel Phytocystatin Gene from Turmeric, Curcuma longa
    Article Snippet: .. Comparison of Total RNA Extraction with Different Methods Total RNA of turmeric (Curcuma longa) was successfully extracted from mature leaves of turmeric plant by three different methods: (i) modified CTAB (cetyltrimethyl ammonium bromide) method, (ii) RNAzol RT (Molecular Research Center Inc., USA), and (iii) RNeasy Plant Mini kit (Qiagen, USA). .. Intact bands of the 28S and 18S RNA can be clearly observed in the agarose gel for all the three methods ( ).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Biosynthesis of Lipoic Acid in Arabidopsis: Cloning and Characterization of the cDNA for Lipoic Acid Synthase 1
    Article Snippet: .. Total RNAs used for cDNA synthesis and RT-PCR were extracted from leaves, roots, and flowers of Arabidopsis using an RNA-extraction kit (RNeasy plant kit, Qiagen, Chatsworth, CA). .. RT-PCR analysis of the LIP1 gene was performed with an RT-PCR kit (SuperScript OneStep System, Life Technologies) using the following two primers: 5′-TTGGGGATACATGTACACGC-3′ and 5′-CTGAATCCCATTTCCATGCC-3′.

    Synthesized:

    Article Title: Multiplex Detection of Aspergillus fumigatus Mycoviruses
    Article Snippet: .. RNA Extraction and Reverse Transcription In order to perform multiplex PCR for mycovirus-infected A. fumigatus isolates, total fungal RNA was extracted using the RNeasy Plant Mini kit (Qiagen, Hilden, Germany) from 100 mg of grounded mycelium, quantified by using NanoDrop 2000C spectrophotometer (Thermo Fischer, Waltham, MA, USA) and cDNA was synthesized using Superscript-III first-strand synthesis system (Invitrogen, Carlsbad, CA, USA) according to the manufacturers’ protocol as follows; 5 µg of RNA, 10 mM of dNTP mix and 250 ng of random primers along with 6.5 µL of DEPC-treated water were incubated at 65 °C for 5 min and snap cooled on ice for at least 2 min. Then 4 µL of 5× first strand buffer, 0.1 M DTT, 40 U of RNasin RNase inhibitor (Promega, Madison, WI, USA) and 200 U of Superscript-III reverse transcriptase were added to the reaction after a brief centrifugation. .. The 20 µL reaction mixture was then subjected to the following cycling regime of incubation at 25 °C, 50 °C and 70 °C for 5 min, 1 h and 15 min, respectively in a DNA Engine DYAD thermocycler.

    Multiplex Assay:

    Article Title: Multiplex Detection of Aspergillus fumigatus Mycoviruses
    Article Snippet: .. RNA Extraction and Reverse Transcription In order to perform multiplex PCR for mycovirus-infected A. fumigatus isolates, total fungal RNA was extracted using the RNeasy Plant Mini kit (Qiagen, Hilden, Germany) from 100 mg of grounded mycelium, quantified by using NanoDrop 2000C spectrophotometer (Thermo Fischer, Waltham, MA, USA) and cDNA was synthesized using Superscript-III first-strand synthesis system (Invitrogen, Carlsbad, CA, USA) according to the manufacturers’ protocol as follows; 5 µg of RNA, 10 mM of dNTP mix and 250 ng of random primers along with 6.5 µL of DEPC-treated water were incubated at 65 °C for 5 min and snap cooled on ice for at least 2 min. Then 4 µL of 5× first strand buffer, 0.1 M DTT, 40 U of RNasin RNase inhibitor (Promega, Madison, WI, USA) and 200 U of Superscript-III reverse transcriptase were added to the reaction after a brief centrifugation. .. The 20 µL reaction mixture was then subjected to the following cycling regime of incubation at 25 °C, 50 °C and 70 °C for 5 min, 1 h and 15 min, respectively in a DNA Engine DYAD thermocycler.

    Spectrophotometry:

    Article Title: Multiplex Detection of Aspergillus fumigatus Mycoviruses
    Article Snippet: .. RNA Extraction and Reverse Transcription In order to perform multiplex PCR for mycovirus-infected A. fumigatus isolates, total fungal RNA was extracted using the RNeasy Plant Mini kit (Qiagen, Hilden, Germany) from 100 mg of grounded mycelium, quantified by using NanoDrop 2000C spectrophotometer (Thermo Fischer, Waltham, MA, USA) and cDNA was synthesized using Superscript-III first-strand synthesis system (Invitrogen, Carlsbad, CA, USA) according to the manufacturers’ protocol as follows; 5 µg of RNA, 10 mM of dNTP mix and 250 ng of random primers along with 6.5 µL of DEPC-treated water were incubated at 65 °C for 5 min and snap cooled on ice for at least 2 min. Then 4 µL of 5× first strand buffer, 0.1 M DTT, 40 U of RNasin RNase inhibitor (Promega, Madison, WI, USA) and 200 U of Superscript-III reverse transcriptase were added to the reaction after a brief centrifugation. .. The 20 µL reaction mixture was then subjected to the following cycling regime of incubation at 25 °C, 50 °C and 70 °C for 5 min, 1 h and 15 min, respectively in a DNA Engine DYAD thermocycler.

    Purification:

    Article Title: Filter paper-based spin column method for cost-efficient DNA or RNA purification
    Article Snippet: .. Purification of plant RNA Plant total RNAs were purified using filter paper-based spin columns following the protocol of the Qiagen RNeasy Plant mini kit (RNeasy Mini Handbook. ..

    Polymerase Chain Reaction:

    Article Title: Multiplex Detection of Aspergillus fumigatus Mycoviruses
    Article Snippet: .. RNA Extraction and Reverse Transcription In order to perform multiplex PCR for mycovirus-infected A. fumigatus isolates, total fungal RNA was extracted using the RNeasy Plant Mini kit (Qiagen, Hilden, Germany) from 100 mg of grounded mycelium, quantified by using NanoDrop 2000C spectrophotometer (Thermo Fischer, Waltham, MA, USA) and cDNA was synthesized using Superscript-III first-strand synthesis system (Invitrogen, Carlsbad, CA, USA) according to the manufacturers’ protocol as follows; 5 µg of RNA, 10 mM of dNTP mix and 250 ng of random primers along with 6.5 µL of DEPC-treated water were incubated at 65 °C for 5 min and snap cooled on ice for at least 2 min. Then 4 µL of 5× first strand buffer, 0.1 M DTT, 40 U of RNasin RNase inhibitor (Promega, Madison, WI, USA) and 200 U of Superscript-III reverse transcriptase were added to the reaction after a brief centrifugation. .. The 20 µL reaction mixture was then subjected to the following cycling regime of incubation at 25 °C, 50 °C and 70 °C for 5 min, 1 h and 15 min, respectively in a DNA Engine DYAD thermocycler.

    Article Title: Multiplex Detection of Aspergillus fumigatus Mycoviruses
    Article Snippet: .. The effect of the dsRNA extraction method on the efficiency of PCR was tested using LiCl extraction and the RNeasy Plant Mini kit (Qiagen). ..

    Incubation:

    Article Title: Multiplex Detection of Aspergillus fumigatus Mycoviruses
    Article Snippet: .. RNA Extraction and Reverse Transcription In order to perform multiplex PCR for mycovirus-infected A. fumigatus isolates, total fungal RNA was extracted using the RNeasy Plant Mini kit (Qiagen, Hilden, Germany) from 100 mg of grounded mycelium, quantified by using NanoDrop 2000C spectrophotometer (Thermo Fischer, Waltham, MA, USA) and cDNA was synthesized using Superscript-III first-strand synthesis system (Invitrogen, Carlsbad, CA, USA) according to the manufacturers’ protocol as follows; 5 µg of RNA, 10 mM of dNTP mix and 250 ng of random primers along with 6.5 µL of DEPC-treated water were incubated at 65 °C for 5 min and snap cooled on ice for at least 2 min. Then 4 µL of 5× first strand buffer, 0.1 M DTT, 40 U of RNasin RNase inhibitor (Promega, Madison, WI, USA) and 200 U of Superscript-III reverse transcriptase were added to the reaction after a brief centrifugation. .. The 20 µL reaction mixture was then subjected to the following cycling regime of incubation at 25 °C, 50 °C and 70 °C for 5 min, 1 h and 15 min, respectively in a DNA Engine DYAD thermocycler.

    Modification:

    Article Title: Molecular Cloning and Characterization of Novel Phytocystatin Gene from Turmeric, Curcuma longa
    Article Snippet: .. Comparison of Total RNA Extraction with Different Methods Total RNA of turmeric (Curcuma longa) was successfully extracted from mature leaves of turmeric plant by three different methods: (i) modified CTAB (cetyltrimethyl ammonium bromide) method, (ii) RNAzol RT (Molecular Research Center Inc., USA), and (iii) RNeasy Plant Mini kit (Qiagen, USA). .. Intact bands of the 28S and 18S RNA can be clearly observed in the agarose gel for all the three methods ( ).

    Centrifugation:

    Article Title: Multiplex Detection of Aspergillus fumigatus Mycoviruses
    Article Snippet: .. RNA Extraction and Reverse Transcription In order to perform multiplex PCR for mycovirus-infected A. fumigatus isolates, total fungal RNA was extracted using the RNeasy Plant Mini kit (Qiagen, Hilden, Germany) from 100 mg of grounded mycelium, quantified by using NanoDrop 2000C spectrophotometer (Thermo Fischer, Waltham, MA, USA) and cDNA was synthesized using Superscript-III first-strand synthesis system (Invitrogen, Carlsbad, CA, USA) according to the manufacturers’ protocol as follows; 5 µg of RNA, 10 mM of dNTP mix and 250 ng of random primers along with 6.5 µL of DEPC-treated water were incubated at 65 °C for 5 min and snap cooled on ice for at least 2 min. Then 4 µL of 5× first strand buffer, 0.1 M DTT, 40 U of RNasin RNase inhibitor (Promega, Madison, WI, USA) and 200 U of Superscript-III reverse transcriptase were added to the reaction after a brief centrifugation. .. The 20 µL reaction mixture was then subjected to the following cycling regime of incubation at 25 °C, 50 °C and 70 °C for 5 min, 1 h and 15 min, respectively in a DNA Engine DYAD thermocycler.

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  • 99
    Qiagen rna extraction kit
    Organ-specific expression of the LIP1 gene. The level of LIP1 mRNA for lipoic acid synthase was analyzed by <t>RT-PCR</t> with total RNAs extracted from leaves (lane 2), roots (lane 3), and flowers (lane 4). In lane 1, genomic DNA was used as the template for RT-PCR instead of <t>RNA.</t> The level of mRNA for the cytosolic form of cyclophilin was also analyzed as a control with the same total RNAs extracted from leaves (lane 5), roots (lane 6), and flowers (lane 7). The positions of the DNA size markers (in kb) are indicated on the left.
    Rna Extraction Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 749 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rna extraction kit/product/Qiagen
    Average 99 stars, based on 749 article reviews
    Price from $9.99 to $1999.99
    rna extraction kit - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    Image Search Results


    Organ-specific expression of the LIP1 gene. The level of LIP1 mRNA for lipoic acid synthase was analyzed by RT-PCR with total RNAs extracted from leaves (lane 2), roots (lane 3), and flowers (lane 4). In lane 1, genomic DNA was used as the template for RT-PCR instead of RNA. The level of mRNA for the cytosolic form of cyclophilin was also analyzed as a control with the same total RNAs extracted from leaves (lane 5), roots (lane 6), and flowers (lane 7). The positions of the DNA size markers (in kb) are indicated on the left.

    Journal: Plant Physiology

    Article Title: Biosynthesis of Lipoic Acid in Arabidopsis: Cloning and Characterization of the cDNA for Lipoic Acid Synthase 1

    doi:

    Figure Lengend Snippet: Organ-specific expression of the LIP1 gene. The level of LIP1 mRNA for lipoic acid synthase was analyzed by RT-PCR with total RNAs extracted from leaves (lane 2), roots (lane 3), and flowers (lane 4). In lane 1, genomic DNA was used as the template for RT-PCR instead of RNA. The level of mRNA for the cytosolic form of cyclophilin was also analyzed as a control with the same total RNAs extracted from leaves (lane 5), roots (lane 6), and flowers (lane 7). The positions of the DNA size markers (in kb) are indicated on the left.

    Article Snippet: Total RNAs used for cDNA synthesis and RT-PCR were extracted from leaves, roots, and flowers of Arabidopsis using an RNA-extraction kit (RNeasy plant kit, Qiagen, Chatsworth, CA).

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction

    Profile of total RNA isolated by using five commercial kits. a Denaturing gel electrophoresis of total RNA isolated from peach. b Denaturing gel electrophoresis of total RNA from grapevine leaves. 50 mg of young peach and grapevine leaves (indicated as Y), and mature (M) grapevine leaves was used in RNA isolation with Spectrum™ Plant Total RNA kit (Sigma), RNeasy Plant mini kit (Qiagen), Plant/fungi total RNA kit (Norgen), AccuPrep viral RNA extraction kit (Bioneer) and TRIzol Reagent (Life Technologies). The total RNA yield (μg), A260/A280 and A260/A230 ratios averaged from two replicates are given below each gel panel. 28S rRNA, 18S rRNA and small RNAs are indicated with arrows. c Capillary electrophoresis of total RNA with Agilent Bioanalyzer. One ml of each of the total RNA preparations isolated using these five systems was used for the analysis with an Agilent Bioanalyzer 2100 equipped with an RNA Nano chip

    Journal: Virology Journal

    Article Title: A highly effective and versatile technology for the isolation of RNAs from grapevines and other woody perennials for use in virus diagnostics

    doi: 10.1186/s12985-015-0376-3

    Figure Lengend Snippet: Profile of total RNA isolated by using five commercial kits. a Denaturing gel electrophoresis of total RNA isolated from peach. b Denaturing gel electrophoresis of total RNA from grapevine leaves. 50 mg of young peach and grapevine leaves (indicated as Y), and mature (M) grapevine leaves was used in RNA isolation with Spectrum™ Plant Total RNA kit (Sigma), RNeasy Plant mini kit (Qiagen), Plant/fungi total RNA kit (Norgen), AccuPrep viral RNA extraction kit (Bioneer) and TRIzol Reagent (Life Technologies). The total RNA yield (μg), A260/A280 and A260/A230 ratios averaged from two replicates are given below each gel panel. 28S rRNA, 18S rRNA and small RNAs are indicated with arrows. c Capillary electrophoresis of total RNA with Agilent Bioanalyzer. One ml of each of the total RNA preparations isolated using these five systems was used for the analysis with an Agilent Bioanalyzer 2100 equipped with an RNA Nano chip

    Article Snippet: These five kits are TRIzol Reagent (Life Technologies), RNeasy Plant mini kit (Qiagen), Spectrum™ Plant Total RNA kit (Sigma), AccuPrep viral RNA extraction kit (Bioneer) and Plant/fungi total RNA kit (Norgen BioTek).

    Techniques: Isolation, Nucleic Acid Electrophoresis, RNA Extraction, Electrophoresis, Chromatin Immunoprecipitation

    Total RNA integrity test on 1.0% agarose gel. Intact 28S and 18S total RNA bands can be observed on the agarose gel, indicating a good integrity of the total RNA after being extracted by different methods (lanes 1 and 2: modified CTAB method, lane 3, 4: RNAzol RT, and lane 5, 6: RNeasy Plant Mini kit).

    Journal: BioMed Research International

    Article Title: Molecular Cloning and Characterization of Novel Phytocystatin Gene from Turmeric, Curcuma longa

    doi: 10.1155/2014/973790

    Figure Lengend Snippet: Total RNA integrity test on 1.0% agarose gel. Intact 28S and 18S total RNA bands can be observed on the agarose gel, indicating a good integrity of the total RNA after being extracted by different methods (lanes 1 and 2: modified CTAB method, lane 3, 4: RNAzol RT, and lane 5, 6: RNeasy Plant Mini kit).

    Article Snippet: Comparison of Total RNA Extraction with Different Methods Total RNA of turmeric (Curcuma longa) was successfully extracted from mature leaves of turmeric plant by three different methods: (i) modified CTAB (cetyltrimethyl ammonium bromide) method, (ii) RNAzol RT (Molecular Research Center Inc., USA), and (iii) RNeasy Plant Mini kit (Qiagen, USA).

    Techniques: Agarose Gel Electrophoresis, Modification

    Conventional PCR to check amplicon size prior to multiplex PCR. Amplicon sizes were checked on two percent agarose gel prior to performing multiplex PCR for A. fumigatus dsRNA mycoviruses. AfuCV, AfuPV-1 and AfuTmV-1 dsRNAs were extracted using LiCl extraction (Lanes 2, 4 and 6, respectively) and RNeasy Plant Mini kit (Lanes 3, 5 and 7, respectively) and used as templates for amplification with AfuCV, AfuPV-1 and AfuTmV-1 primers (2–3, 4–5, 6–7, respectively). Hyperladder-I was used as a marker to estimate the size of the amplicons (Lane 1).

    Journal: Viruses

    Article Title: Multiplex Detection of Aspergillus fumigatus Mycoviruses

    doi: 10.3390/v10050247

    Figure Lengend Snippet: Conventional PCR to check amplicon size prior to multiplex PCR. Amplicon sizes were checked on two percent agarose gel prior to performing multiplex PCR for A. fumigatus dsRNA mycoviruses. AfuCV, AfuPV-1 and AfuTmV-1 dsRNAs were extracted using LiCl extraction (Lanes 2, 4 and 6, respectively) and RNeasy Plant Mini kit (Lanes 3, 5 and 7, respectively) and used as templates for amplification with AfuCV, AfuPV-1 and AfuTmV-1 primers (2–3, 4–5, 6–7, respectively). Hyperladder-I was used as a marker to estimate the size of the amplicons (Lane 1).

    Article Snippet: RNA Extraction and Reverse Transcription In order to perform multiplex PCR for mycovirus-infected A. fumigatus isolates, total fungal RNA was extracted using the RNeasy Plant Mini kit (Qiagen, Hilden, Germany) from 100 mg of grounded mycelium, quantified by using NanoDrop 2000C spectrophotometer (Thermo Fischer, Waltham, MA, USA) and cDNA was synthesized using Superscript-III first-strand synthesis system (Invitrogen, Carlsbad, CA, USA) according to the manufacturers’ protocol as follows; 5 µg of RNA, 10 mM of dNTP mix and 250 ng of random primers along with 6.5 µL of DEPC-treated water were incubated at 65 °C for 5 min and snap cooled on ice for at least 2 min. Then 4 µL of 5× first strand buffer, 0.1 M DTT, 40 U of RNasin RNase inhibitor (Promega, Madison, WI, USA) and 200 U of Superscript-III reverse transcriptase were added to the reaction after a brief centrifugation.

    Techniques: Polymerase Chain Reaction, Amplification, Multiplex Assay, Agarose Gel Electrophoresis, Marker