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Illumina Inc rna oligonucleotides
Rna Oligonucleotides, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rna oligonucleotides/product/Illumina Inc
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
rna oligonucleotides - by Bioz Stars, 2020-04
94/100 stars

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Sequencing:

Article Title: Non-templated addition and template switching by Moloney murine leukemia virus (MMLV)-based reverse transcriptases co-occur and compete with each other
Article Snippet: Paragraph title: The template switching bias is marginally affected by the internal RNA sequence ... We then performed the template-switching reverse transcription on these RNA oligonucleotides and constructed Illumina libraries to determine the extent of biases ( , B–D ).

Synthesized:

Article Title: Non-templated addition and template switching by Moloney murine leukemia virus (MMLV)-based reverse transcriptases co-occur and compete with each other
Article Snippet: The template switching bias is marginally affected by the internal RNA sequence To further analyze the effect of the internal RNA sequence on the bias of template switching, we synthesized RNA oligonucleotides with the first four nucleotides randomized. .. We then performed the template-switching reverse transcription on these RNA oligonucleotides and constructed Illumina libraries to determine the extent of biases ( , B–D ).

Construct:

Article Title: Non-templated addition and template switching by Moloney murine leukemia virus (MMLV)-based reverse transcriptases co-occur and compete with each other
Article Snippet: .. We then performed the template-switching reverse transcription on these RNA oligonucleotides and constructed Illumina libraries to determine the extent of biases ( , B–D ). .. The sequencing data from template switching experiments were normalized to the relative composition of the first four ribonucleotides in the respective synthetic templates.

Blocking Assay:

Article Title: Low-cost cross-taxon enrichment of mitochondrial DNA using in-house synthesised RNA probes
Article Snippet: .. Prior to hybridization capture, the following two solutions were prepared: Probe Solution: 9 μL 20x SSPE, 0.5 μL 0.5 M EDTA pH 8.0, 3.5 μL 50X Denhardt’s Solution, 0.5 μL 10% SDS, 1 μL SUPERase-In (20 U/μL, ThermoFisher), 125 ng in-house baits, 0.5 μL 50 μM RNA oligos to block truncated Illumina adapters [ ], and nuclease-free H2 O to 20 μL. .. Target Solution: 100 ng truncated Illumina library, 2.5 μL 1 μg/μL human Cot-1 DNA, 2.5 μL 1 μg/μL salmon sperm DNA, and nuclease-free H2 O to 12 μL.

Hybridization:

Article Title: Low-cost cross-taxon enrichment of mitochondrial DNA using in-house synthesised RNA probes
Article Snippet: .. Prior to hybridization capture, the following two solutions were prepared: Probe Solution: 9 μL 20x SSPE, 0.5 μL 0.5 M EDTA pH 8.0, 3.5 μL 50X Denhardt’s Solution, 0.5 μL 10% SDS, 1 μL SUPERase-In (20 U/μL, ThermoFisher), 125 ng in-house baits, 0.5 μL 50 μM RNA oligos to block truncated Illumina adapters [ ], and nuclease-free H2 O to 20 μL. .. Target Solution: 100 ng truncated Illumina library, 2.5 μL 1 μg/μL human Cot-1 DNA, 2.5 μL 1 μg/μL salmon sperm DNA, and nuclease-free H2 O to 12 μL.

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  • 99
    Illumina Inc 16s rrna gene
    Hh infection induces IL-22 dependent dysbiosis (A) Quantification of Hh colonization density in the cecal content. n=9-11. (B) Chao and Shannon diversity indices from <t>16S</t> <t>rRNA</t> sequencing data obtained from fecal pellets of uninfected mice and Hh infected mice treated with control or anti-IL-22 antibody as indicated. (C) Relative abundance of indicated taxa from groups described in B. (D) Relative abundance of indicated taxa as determined by taxa specific 16s rRNA qPCR from groups described in B.
    16s Rrna Gene, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 1085 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/16s rrna gene/product/Illumina Inc
    Average 99 stars, based on 1085 article reviews
    Price from $9.99 to $1999.99
    16s rrna gene - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    94
    Illumina Inc ns 35s hsfa1b plants
    <t>HSFA1b</t> regulates expression of cis NAT genes and their target sense genes. (A) Heat map showing differentially expressed cis NAT genes and their putative sense targets in WT plants in HS compared with NS and in <t>35S:HSFA1b</t> NS plants compared with WT NS plants. (B) Linear correlation plots showing the relationship between cis NAT and sense target transcript abundance for WT plants subjected to HS compared with NS (top panel) and for 35S:HSFA1b NS plants compared with WT NS plants (bottom panel). (C) Transcript levels determined by qPCR of selected cis NAT genes and their TF gene sense target in HS and NS qKO plants compared with their parental genotypes as in the legend of Fig. 5B . The suffixes ‘a’ and ‘b’ are where the qKO mutant shows a significant difference ( P
    Ns 35s Hsfa1b Plants, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ns 35s hsfa1b plants/product/Illumina Inc
    Average 94 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    ns 35s hsfa1b plants - by Bioz Stars, 2020-04
    94/100 stars
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    99
    Illumina Inc 16s rrna gene sequencing
    Phylogenetic classification of amplified archaeal <t>16S</t> <t>rRNA</t> genes in the methanogenic incubations. The maximum taxonomy depth is on family level. Taxonomic groups with
    16s Rrna Gene Sequencing, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 262 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/16s rrna gene sequencing/product/Illumina Inc
    Average 99 stars, based on 262 article reviews
    Price from $9.99 to $1999.99
    16s rrna gene sequencing - by Bioz Stars, 2020-04
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    93
    Illumina Inc earth microbiome project primer set
    Early HMOS dietary intervention changes the alpha diversity and beta diversity of fecal <t>microbiota</t> of the non-diabetic and diabetic mice within each group at four collection points. ( A ) richness. ( B ) Shannon’s index. ( C ) Simpson’s index. ( D ) evenness. Alpha diversity indices data are represented as mean ± SEM, n = 17–20/ group, *p
    Earth Microbiome Project Primer Set, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/earth microbiome project primer set/product/Illumina Inc
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    earth microbiome project primer set - by Bioz Stars, 2020-04
    93/100 stars
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    Image Search Results


    Hh infection induces IL-22 dependent dysbiosis (A) Quantification of Hh colonization density in the cecal content. n=9-11. (B) Chao and Shannon diversity indices from 16S rRNA sequencing data obtained from fecal pellets of uninfected mice and Hh infected mice treated with control or anti-IL-22 antibody as indicated. (C) Relative abundance of indicated taxa from groups described in B. (D) Relative abundance of indicated taxa as determined by taxa specific 16s rRNA qPCR from groups described in B.

    Journal: Mucosal immunology

    Article Title: Interleukin-22 drives nitric oxide-dependent DNA damage and dysplasia in a murine model of colitis-associated cancer

    doi: 10.1038/mi.2017.9

    Figure Lengend Snippet: Hh infection induces IL-22 dependent dysbiosis (A) Quantification of Hh colonization density in the cecal content. n=9-11. (B) Chao and Shannon diversity indices from 16S rRNA sequencing data obtained from fecal pellets of uninfected mice and Hh infected mice treated with control or anti-IL-22 antibody as indicated. (C) Relative abundance of indicated taxa from groups described in B. (D) Relative abundance of indicated taxa as determined by taxa specific 16s rRNA qPCR from groups described in B.

    Article Snippet: Amplicons were generated using oligonucleotide primers that target approximately 300 bp of the V4 variable region of the 16S rRNA gene (primers 515F and 806R) and also were barcoded and pooled to construct the sequencing library, followed by sequencing with an Illumina MiSeq instrument to generate paired-end 150×150 reads.

    Techniques: Infection, Sequencing, Mouse Assay, Real-time Polymerase Chain Reaction

    HSFA1b regulates expression of cis NAT genes and their target sense genes. (A) Heat map showing differentially expressed cis NAT genes and their putative sense targets in WT plants in HS compared with NS and in 35S:HSFA1b NS plants compared with WT NS plants. (B) Linear correlation plots showing the relationship between cis NAT and sense target transcript abundance for WT plants subjected to HS compared with NS (top panel) and for 35S:HSFA1b NS plants compared with WT NS plants (bottom panel). (C) Transcript levels determined by qPCR of selected cis NAT genes and their TF gene sense target in HS and NS qKO plants compared with their parental genotypes as in the legend of Fig. 5B . The suffixes ‘a’ and ‘b’ are where the qKO mutant shows a significant difference ( P

    Journal: Journal of Experimental Botany

    Article Title: Arabidopsis HEAT SHOCK TRANSCRIPTION FACTORA1b regulates multiple developmental genes under benign and stress conditions

    doi: 10.1093/jxb/ery142

    Figure Lengend Snippet: HSFA1b regulates expression of cis NAT genes and their target sense genes. (A) Heat map showing differentially expressed cis NAT genes and their putative sense targets in WT plants in HS compared with NS and in 35S:HSFA1b NS plants compared with WT NS plants. (B) Linear correlation plots showing the relationship between cis NAT and sense target transcript abundance for WT plants subjected to HS compared with NS (top panel) and for 35S:HSFA1b NS plants compared with WT NS plants (bottom panel). (C) Transcript levels determined by qPCR of selected cis NAT genes and their TF gene sense target in HS and NS qKO plants compared with their parental genotypes as in the legend of Fig. 5B . The suffixes ‘a’ and ‘b’ are where the qKO mutant shows a significant difference ( P

    Article Snippet: RNA-seq data analysis RNA was extracted from NS WT, HS WT, and NS 35S:HSFA1b plants (three biological replicates of each) as described previously ( ) and analysed using massively parallel sequencing (RNA-seq) as follows: synthesis of cDNA, library preparation, and sequencing were carried out at Earlham Institute on the Illumina HiSeq2000 platform using 50 bp paired-end sequencing with minimum read depth ≥25 million reads per library.

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Mutagenesis

    HSFA1b can indirectly control expression through its regulation of TF gene expression. (A) Heat map comparing normalized FPKM values for 28 TF genes bound by HSFA1b and differentially expressed in 35S:HSFA1b and WT HS plants. Asterisks (*) indicate development-associated TF genes. (B) Confirmation of the regulation of selected TF genes by clade A1 HSF genes. Quantitative real-time RT–PCR was conducted on RNA from qKO rosettes under NS and HS in comparison with its parental genotypes Col-0 (C) and Ws-0 (W). The suffixes ‘a’ and ‘b’ are where the qKO mutant shows a significant difference ( P

    Journal: Journal of Experimental Botany

    Article Title: Arabidopsis HEAT SHOCK TRANSCRIPTION FACTORA1b regulates multiple developmental genes under benign and stress conditions

    doi: 10.1093/jxb/ery142

    Figure Lengend Snippet: HSFA1b can indirectly control expression through its regulation of TF gene expression. (A) Heat map comparing normalized FPKM values for 28 TF genes bound by HSFA1b and differentially expressed in 35S:HSFA1b and WT HS plants. Asterisks (*) indicate development-associated TF genes. (B) Confirmation of the regulation of selected TF genes by clade A1 HSF genes. Quantitative real-time RT–PCR was conducted on RNA from qKO rosettes under NS and HS in comparison with its parental genotypes Col-0 (C) and Ws-0 (W). The suffixes ‘a’ and ‘b’ are where the qKO mutant shows a significant difference ( P

    Article Snippet: RNA-seq data analysis RNA was extracted from NS WT, HS WT, and NS 35S:HSFA1b plants (three biological replicates of each) as described previously ( ) and analysed using massively parallel sequencing (RNA-seq) as follows: synthesis of cDNA, library preparation, and sequencing were carried out at Earlham Institute on the Illumina HiSeq2000 platform using 50 bp paired-end sequencing with minimum read depth ≥25 million reads per library.

    Techniques: Expressing, Quantitative RT-PCR, Mutagenesis

    35S:HSFA1b plants under NS partially mimic HS WT plants. (A) Heat map comparing normalized FPKM values for HSP genes in NS and HS WT and 35S:HSFA1b NS plants. (B) GO Slim analysis comparing the enriched Biological Process terms (Benjamini–Hochberg FDR

    Journal: Journal of Experimental Botany

    Article Title: Arabidopsis HEAT SHOCK TRANSCRIPTION FACTORA1b regulates multiple developmental genes under benign and stress conditions

    doi: 10.1093/jxb/ery142

    Figure Lengend Snippet: 35S:HSFA1b plants under NS partially mimic HS WT plants. (A) Heat map comparing normalized FPKM values for HSP genes in NS and HS WT and 35S:HSFA1b NS plants. (B) GO Slim analysis comparing the enriched Biological Process terms (Benjamini–Hochberg FDR

    Article Snippet: RNA-seq data analysis RNA was extracted from NS WT, HS WT, and NS 35S:HSFA1b plants (three biological replicates of each) as described previously ( ) and analysed using massively parallel sequencing (RNA-seq) as follows: synthesis of cDNA, library preparation, and sequencing were carried out at Earlham Institute on the Illumina HiSeq2000 platform using 50 bp paired-end sequencing with minimum read depth ≥25 million reads per library.

    Techniques:

    Phylogenetic classification of amplified archaeal 16S rRNA genes in the methanogenic incubations. The maximum taxonomy depth is on family level. Taxonomic groups with

    Journal: Environmental Microbiology

    Article Title: Increases in temperature and nutrient availability positively affect methane‐cycling microorganisms in Arctic thermokarst lake sediments

    doi: 10.1111/1462-2920.14345

    Figure Lengend Snippet: Phylogenetic classification of amplified archaeal 16S rRNA genes in the methanogenic incubations. The maximum taxonomy depth is on family level. Taxonomic groups with

    Article Snippet: Recently it was found that the forward primer Arc349F, mostly used for Illumina 16S rRNA gene sequencing of Archaea and also used in this study, only matches 53% of Archaea and missed almost all Crenarchaea and unclassified Archaea (Hugerth et al ., ).

    Techniques: Amplification

    Early HMOS dietary intervention changes the alpha diversity and beta diversity of fecal microbiota of the non-diabetic and diabetic mice within each group at four collection points. ( A ) richness. ( B ) Shannon’s index. ( C ) Simpson’s index. ( D ) evenness. Alpha diversity indices data are represented as mean ± SEM, n = 17–20/ group, *p

    Journal: Scientific Reports

    Article Title: Human milk oligosaccharides protect against the development of autoimmune diabetes in NOD-mice

    doi: 10.1038/s41598-018-22052-y

    Figure Lengend Snippet: Early HMOS dietary intervention changes the alpha diversity and beta diversity of fecal microbiota of the non-diabetic and diabetic mice within each group at four collection points. ( A ) richness. ( B ) Shannon’s index. ( C ) Simpson’s index. ( D ) evenness. Alpha diversity indices data are represented as mean ± SEM, n = 17–20/ group, *p

    Article Snippet: Total DNA was extracted from NOD-mice fecal samples (FastDNA bead-beating Spin Kit for Soil, MP Biomedicals, Solon, OH), amplified the V4 variable region of the microbial 16 S rRNA gene (Earth Microbiome Project primer set, adapted for the Illumina platform) , and sequenced on an Illumina MiSeq (2 × 151 bp reads) at Argonne National Laboratory.

    Techniques: Mouse Assay

    Early HMOS dietary intervention alters fecal microbiota composition over time. Fecal samples at Wk4 (baseline), Wk9 (start intervention), Wk14 (post-intervention) and Wk30 (endpoint) were analyzed using 16 S rRNA. ( A ) Phylum level pie charts, organized by weekly collection time points, significance indicated either by: ( # )False Discovery Rate (FDR) p

    Journal: Scientific Reports

    Article Title: Human milk oligosaccharides protect against the development of autoimmune diabetes in NOD-mice

    doi: 10.1038/s41598-018-22052-y

    Figure Lengend Snippet: Early HMOS dietary intervention alters fecal microbiota composition over time. Fecal samples at Wk4 (baseline), Wk9 (start intervention), Wk14 (post-intervention) and Wk30 (endpoint) were analyzed using 16 S rRNA. ( A ) Phylum level pie charts, organized by weekly collection time points, significance indicated either by: ( # )False Discovery Rate (FDR) p

    Article Snippet: Total DNA was extracted from NOD-mice fecal samples (FastDNA bead-beating Spin Kit for Soil, MP Biomedicals, Solon, OH), amplified the V4 variable region of the microbial 16 S rRNA gene (Earth Microbiome Project primer set, adapted for the Illumina platform) , and sequenced on an Illumina MiSeq (2 × 151 bp reads) at Argonne National Laboratory.

    Techniques: