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Agilent technologies rna nano 6000 assay kit
Rna Nano 6000 Assay Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 128 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rna nano 6000 assay kit/product/Agilent technologies
Average 99 stars, based on 128 article reviews
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rna nano 6000 assay kit - by Bioz Stars, 2019-10
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Amplification:

Article Title: Molecular mechanisms of tannin accumulation in Rhus galls and genes involved in plant-insect interactions
Article Snippet: RNA purity and integrity were assessed using a RNA Nano 6000 Assay Kit (Agilent Technologies, CA, USA). .. The double-stranded cDNA was synthesized and purified using a QiaQuick PCR Extraction kit (QIAGEN, MD, USA) and washed using EB buffer for end repair and the addition of single adenines.

Article Title: Application of RNA-seq for mitogenome reconstruction, and reconsideration of long-branch artifacts in Hemiptera phylogeny
Article Snippet: Finally, the cDNA fragments were enriched by PCR amplification using random hexamers, and products were purified by AMPure XP beads to generate final library. .. The size and purity of the cDNA sequencing libraries were determined using a 2100 Bioanalyzer with the RNA Nano 6000 Assay Kit (Agilent Technologies, CA, USA) and the quantity was estimated using Q-PCR.

Synthesized:

Article Title: Transcriptome sequencing identifies key pathways and genes involved in gastric adenocarcinoma
Article Snippet: RNA integrity was detected using the RNA Nano 6000 Assay kit (Agilent Technologies, Inc., Santa Clara, CA, USA). .. RNA integrity was detected using the RNA Nano 6000 Assay kit (Agilent Technologies, Inc., Santa Clara, CA, USA).

Article Title: Molecular mechanisms of tannin accumulation in Rhus galls and genes involved in plant-insect interactions
Article Snippet: RNA purity and integrity were assessed using a RNA Nano 6000 Assay Kit (Agilent Technologies, CA, USA). .. First strand cDNA was synthesized with random hexamers as primers and mRNA fragments as templates.

Article Title: Comparative transcriptome analysis of Rimicaris sp. reveals novel molecular features associated with survival in deep-sea hydrothermal vent
Article Snippet: RNA integrity was assessed by using a RNA Nano 6000 assay kit (Agilent Technologies, CA, USA). .. Briefly, mRNA was purified from total RNA using polyT oligo-attached magnetic beads.

Article Title: Application of RNA-seq for mitogenome reconstruction, and reconsideration of long-branch artifacts in Hemiptera phylogeny
Article Snippet: The RNA templates were then removed using RNaseH and the second strand was synthesized using DNA polymerase I to generate double-stranded cDNA fragments. .. The size and purity of the cDNA sequencing libraries were determined using a 2100 Bioanalyzer with the RNA Nano 6000 Assay Kit (Agilent Technologies, CA, USA) and the quantity was estimated using Q-PCR.

Quantitative RT-PCR:

Article Title: Global and Complement Gene-Specific DNA Methylation in Grass Carp after Grass Carp Reovirus (GCRV) Infection
Article Snippet: Paragraph title: 4.5. RNA Isolation and Quantitative Real-Time PCR (RT-qPCR) ... The Qubit RNA assay kit (Life Technologies, Carlsbad, CA, USA) and RNA Nano 6000 assay kit (Agilent Technologies, Santa Clara, CA, USA) were used to measure the concentration and integrity of isolated RNA, respectively.

Article Title: Identification and characterization of microRNAs in the pituitary of pubescent goats
Article Snippet: RNA integrity for sequencing was assessed using the RNA Nano 6000 Assay Kit and a Agilent Bioanalyzer 2100 system (Agilent Technologies, CA, USA). .. The concentration of total RNA for qRT-PCR was quantifiedc at 260 nm with a NanoDrop spectrophotometer (ND-2000, USA).

SYBR Green Assay:

Article Title: Global and Complement Gene-Specific DNA Methylation in Grass Carp after Grass Carp Reovirus (GCRV) Infection
Article Snippet: The Qubit RNA assay kit (Life Technologies, Carlsbad, CA, USA) and RNA Nano 6000 assay kit (Agilent Technologies, Santa Clara, CA, USA) were used to measure the concentration and integrity of isolated RNA, respectively. .. The Qubit RNA assay kit (Life Technologies, Carlsbad, CA, USA) and RNA Nano 6000 assay kit (Agilent Technologies, Santa Clara, CA, USA) were used to measure the concentration and integrity of isolated RNA, respectively.

Random Hexamer Labeling:

Article Title: Comparative transcriptome analysis of Rimicaris sp. reveals novel molecular features associated with survival in deep-sea hydrothermal vent
Article Snippet: RNA integrity was assessed by using a RNA Nano 6000 assay kit (Agilent Technologies, CA, USA). .. Briefly, mRNA was purified from total RNA using polyT oligo-attached magnetic beads.

Expressing:

Article Title: Deep Illumina sequencing reveals differential expression of long non-coding RNAs in hyperoxia induced bronchopulmonary dysplasia in a rat model
Article Snippet: Agarose gel electrophoresis was applied, and RNA integrity was assessed with the RNA Nano 6000 assay kit (Agilent Technologies, USA). .. The lincRNA samples were subjected to PE150 sequencing according to the HiSeq 3000/4000 PE Cluster Kit instructions and the Hiseq4000 sequencer operating instructions.

Article Title: Global and Complement Gene-Specific DNA Methylation in Grass Carp after Grass Carp Reovirus (GCRV) Infection
Article Snippet: The Qubit RNA assay kit (Life Technologies, Carlsbad, CA, USA) and RNA Nano 6000 assay kit (Agilent Technologies, Santa Clara, CA, USA) were used to measure the concentration and integrity of isolated RNA, respectively. .. The Qubit RNA assay kit (Life Technologies, Carlsbad, CA, USA) and RNA Nano 6000 assay kit (Agilent Technologies, Santa Clara, CA, USA) were used to measure the concentration and integrity of isolated RNA, respectively.

Ligation:

Article Title: Transcriptome sequencing identifies key pathways and genes involved in gastric adenocarcinoma
Article Snippet: RNA integrity was detected using the RNA Nano 6000 Assay kit (Agilent Technologies, Inc., Santa Clara, CA, USA). .. Thereafter, double-stranded cDNA was synthesized and purified with AMPure XP beads.

Generated:

Article Title: Molecular mechanisms of tannin accumulation in Rhus galls and genes involved in plant-insect interactions
Article Snippet: RNA purity and integrity were assessed using a RNA Nano 6000 Assay Kit (Agilent Technologies, CA, USA). .. Sequencing adaptors were ligated to the fragments, and the required fragments were purified by agarose gel electrophoresis and enriched by PCR amplification.

Article Title: Filtered reproductive long non-coding RNAs by genome-wide analyses of goat ovary at different estrus periods
Article Snippet: Finally, the RNA integrity was assessed by the RNA Nano 6000 assay kit (Agilent Technologies, CA, USA) with the Bioanalyzer 2100 system. .. Then, the NEBNext, hyperdirectional RNA library preparation kit (NIB, USA) was used to generate the sequencing library by using rRNA-depleted RNA according to the manufacturer’s instructions.

Article Title: Comparative transcriptome analysis of Rimicaris sp. reveals novel molecular features associated with survival in deep-sea hydrothermal vent
Article Snippet: RNA integrity was assessed by using a RNA Nano 6000 assay kit (Agilent Technologies, CA, USA). .. RNA integrity was assessed by using a RNA Nano 6000 assay kit (Agilent Technologies, CA, USA).

Article Title: Deep RNA sequencing of pectoralis muscle transcriptomes during late-term embryonic to neonatal development in indigenous Chinese duck breeds
Article Snippet: RNA purity and integrity were checked using a Nano Photometer® spectrophotometer (Implen, Westlake Village, CA, USA) and an RNA Nano 6000 Assay Kit (Bioanalyzer 2100 system; Agilent Technologies, Palo Alto, CA, USA), respectively. .. RNA purity and integrity were checked using a Nano Photometer® spectrophotometer (Implen, Westlake Village, CA, USA) and an RNA Nano 6000 Assay Kit (Bioanalyzer 2100 system; Agilent Technologies, Palo Alto, CA, USA), respectively.

Sequencing:

Article Title: Transcriptome sequencing identifies key pathways and genes involved in gastric adenocarcinoma
Article Snippet: Paragraph title: RNA isolation, library preparation, and sequencing ... RNA integrity was detected using the RNA Nano 6000 Assay kit (Agilent Technologies, Inc., Santa Clara, CA, USA).

Article Title: Deep Illumina sequencing reveals differential expression of long non-coding RNAs in hyperoxia induced bronchopulmonary dysplasia in a rat model
Article Snippet: Paragraph title: RNA isolation, library construction, and sequencing ... Agarose gel electrophoresis was applied, and RNA integrity was assessed with the RNA Nano 6000 assay kit (Agilent Technologies, USA).

Article Title: Molecular mechanisms of tannin accumulation in Rhus galls and genes involved in plant-insect interactions
Article Snippet: Paragraph title: Illumina sequencing and transcriptome analysis ... RNA purity and integrity were assessed using a RNA Nano 6000 Assay Kit (Agilent Technologies, CA, USA).

Article Title: Filtered reproductive long non-coding RNAs by genome-wide analyses of goat ovary at different estrus periods
Article Snippet: Finally, the RNA integrity was assessed by the RNA Nano 6000 assay kit (Agilent Technologies, CA, USA) with the Bioanalyzer 2100 system. .. First, the Epicenter Ribo-zero™ rRNA Removal Kit (Epicenter, USA) was used to remove ribosomal RNAs, and rRNA was removed by ethanol precipitation.

Article Title: Comparative transcriptome analysis of Rimicaris sp. reveals novel molecular features associated with survival in deep-sea hydrothermal vent
Article Snippet: RNA integrity was assessed by using a RNA Nano 6000 assay kit (Agilent Technologies, CA, USA). .. RNA integrity was assessed by using a RNA Nano 6000 assay kit (Agilent Technologies, CA, USA).

Article Title: Transcriptome analyses suggest a novel hypothesis for whitefly adaptation to tobacco
Article Snippet: Paragraph title: Sample preparation and Illumina sequencing ... The integrity of the RNA samples was analysed on the 2100 Bioanalyzer with RNA Nano 6000 Assay Kit (Agilent Technologies, USA).

Article Title: Application of RNA-seq for mitogenome reconstruction, and reconsideration of long-branch artifacts in Hemiptera phylogeny
Article Snippet: Finally, the cDNA fragments were enriched by PCR amplification using random hexamers, and products were purified by AMPure XP beads to generate final library. .. The size and purity of the cDNA sequencing libraries were determined using a 2100 Bioanalyzer with the RNA Nano 6000 Assay Kit (Agilent Technologies, CA, USA) and the quantity was estimated using Q-PCR. .. RNA transcript was sequenced on an Illumina Hiseq 2000 in Novogene Bioinformatics Institute (Beijing, China).

Article Title: Identification and characterization of microRNAs in the pituitary of pubescent goats
Article Snippet: Total RNA was isolated using TRIzol (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocol. .. RNA integrity for sequencing was assessed using the RNA Nano 6000 Assay Kit and a Agilent Bioanalyzer 2100 system (Agilent Technologies, CA, USA). .. The concentration of total RNA for qRT-PCR was quantifiedc at 260 nm with a NanoDrop spectrophotometer (ND-2000, USA).

Article Title: Deep RNA sequencing of pectoralis muscle transcriptomes during late-term embryonic to neonatal development in indigenous Chinese duck breeds
Article Snippet: Paragraph title: RNA isolation, library preparation, and sequencing ... RNA purity and integrity were checked using a Nano Photometer® spectrophotometer (Implen, Westlake Village, CA, USA) and an RNA Nano 6000 Assay Kit (Bioanalyzer 2100 system; Agilent Technologies, Palo Alto, CA, USA), respectively.

Article Title: Deep Circular RNA Sequencing Provides Insights into the Mechanism Underlying Grass Carp Reovirus Infection
Article Snippet: Paragraph title: 4.4. RNA Isolation, Library Construction, and Sequencing ... RNA concentration and integrity was measured using the Qubit RNA assay kit (Life Technologies, Carlsbad, CA, USA) and the RNA Nano 6000 assay kit (Agilent Technologies, Santa Clara, CA, USA), respectively.

Injection:

Article Title: Identification and characterization of microRNAs in the pituitary of pubescent goats
Article Snippet: Pituitary glands were collected from pubescent ( n = 3) and prepubescent (n = 3) goats [ , ] after animals were anesthetized with injection of 0.1 ml xylazine hydrochloride (Muhua China, Lot number 150804) before sacrificed. .. RNA integrity for sequencing was assessed using the RNA Nano 6000 Assay Kit and a Agilent Bioanalyzer 2100 system (Agilent Technologies, CA, USA).

RNA Sequencing Assay:

Article Title: RNA-Sequencing and Bioinformatics Analysis of Long Noncoding RNAs and mRNAs in the Prefrontal Cortex of Mice Following Repeated Social Defeat Stress
Article Snippet: Only mice that presented with anxiety-like behavior were subjected to RNA-sequencing and bioinformatics analysis. .. In addition, RNA integrity was assessed with RNA Nano 6000 Assay Kit (Agilent Technologies, Foster City, CA, USA).

Fluorescence:

Article Title: Global and Complement Gene-Specific DNA Methylation in Grass Carp after Grass Carp Reovirus (GCRV) Infection
Article Snippet: The Qubit RNA assay kit (Life Technologies, Carlsbad, CA, USA) and RNA Nano 6000 assay kit (Agilent Technologies, Santa Clara, CA, USA) were used to measure the concentration and integrity of isolated RNA, respectively. .. The Qubit RNA assay kit (Life Technologies, Carlsbad, CA, USA) and RNA Nano 6000 assay kit (Agilent Technologies, Santa Clara, CA, USA) were used to measure the concentration and integrity of isolated RNA, respectively.

Magnetic Beads:

Article Title: Comparative transcriptome analysis of Rimicaris sp. reveals novel molecular features associated with survival in deep-sea hydrothermal vent
Article Snippet: RNA integrity was assessed by using a RNA Nano 6000 assay kit (Agilent Technologies, CA, USA). .. Sequencing libraries were generated using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA), following the manufacturer’s recommendations, and index codes were added to attribute sequences to each sample.

Article Title: Deep RNA sequencing of pectoralis muscle transcriptomes during late-term embryonic to neonatal development in indigenous Chinese duck breeds
Article Snippet: RNA purity and integrity were checked using a Nano Photometer® spectrophotometer (Implen, Westlake Village, CA, USA) and an RNA Nano 6000 Assay Kit (Bioanalyzer 2100 system; Agilent Technologies, Palo Alto, CA, USA), respectively. .. Sequencing libraries were generated using an Illumina Tru Seq™ RNA sample preparation kit (Illumina, San Diego, CA, USA) following the manufacturer’s recommendations, and index codes were added to attribute a sequence to each sample.

Isolation:

Article Title: RNA-Sequencing and Bioinformatics Analysis of Long Noncoding RNAs and mRNAs in the Prefrontal Cortex of Mice Following Repeated Social Defeat Stress
Article Snippet: Paragraph title: 2.4. Tissue Collection and RNA Isolation ... In addition, RNA integrity was assessed with RNA Nano 6000 Assay Kit (Agilent Technologies, Foster City, CA, USA).

Article Title: Transcriptome sequencing identifies key pathways and genes involved in gastric adenocarcinoma
Article Snippet: Paragraph title: RNA isolation, library preparation, and sequencing ... RNA integrity was detected using the RNA Nano 6000 Assay kit (Agilent Technologies, Inc., Santa Clara, CA, USA).

Article Title: Deep Illumina sequencing reveals differential expression of long non-coding RNAs in hyperoxia induced bronchopulmonary dysplasia in a rat model
Article Snippet: Paragraph title: RNA isolation, library construction, and sequencing ... Agarose gel electrophoresis was applied, and RNA integrity was assessed with the RNA Nano 6000 assay kit (Agilent Technologies, USA).

Article Title: Functional Characterization and Evolutionary Analysis of Glycine-Betaine Biosynthesis Pathway in Red Seaweed Pyropia yezoensis
Article Snippet: Paragraph title: 4.2. RNA Isolation and cDNA Synthesis ... RNA integrity was assessed using an RNA Nano 6000 Assay Kit and the Bioanalyzer 2100 system (Agilent Technologies, CA, U.S.).

Article Title: Transcriptomic Analysis of Coding Genes and Non-Coding RNAs Reveals Complex Regulatory Networks Underlying the Black Back and White Belly Coat Phenotype in Chinese Wuzhishan Pigs
Article Snippet: Paragraph title: 2.1. Animal Sample Collection and RNA Isolation ... RNA integrity and concentration was assessed using the RNA Nano 6000 Assay Kit and the Bioanalyzer 2100 system (Agilent Technologies, Foster, CA, USA).

Article Title: Transcriptome analysis of the salivary glands of the grain aphid, Sitobion avenae
Article Snippet: Paragraph title: Sample collection and RNA isolation ... RNA integrity was assessed using the RNA Nano 6000 Assay Kit and the Bioanalyzer 2100 system (Agilent Technologies, CA, USA).

Article Title: Transcriptome analyses suggest a novel hypothesis for whitefly adaptation to tobacco
Article Snippet: Total RNA was isolated from each sample separately using SV Total RNA Isolation System (Promega, USA) following the manufacturer’s protocol. .. The integrity of the RNA samples was analysed on the 2100 Bioanalyzer with RNA Nano 6000 Assay Kit (Agilent Technologies, USA).

Article Title: Global and Complement Gene-Specific DNA Methylation in Grass Carp after Grass Carp Reovirus (GCRV) Infection
Article Snippet: TRizol reagent (Invitrogen, Carlsbad, CA, USA) was used to isolate RNA from spleen samples according the manufacturer’s protocol. .. The Qubit RNA assay kit (Life Technologies, Carlsbad, CA, USA) and RNA Nano 6000 assay kit (Agilent Technologies, Santa Clara, CA, USA) were used to measure the concentration and integrity of isolated RNA, respectively. .. DNase I (Promega, Madison, WI, USA)-treated total RNA was used as the template for the first-strand cDNA synthesis.

Article Title: Identification and characterization of microRNAs in the pituitary of pubescent goats
Article Snippet: Paragraph title: Pituitary collection and total RNA isolation ... RNA integrity for sequencing was assessed using the RNA Nano 6000 Assay Kit and a Agilent Bioanalyzer 2100 system (Agilent Technologies, CA, USA).

Article Title: Coupling Seq-BSA and RNA-Seq Analyses Reveal the Molecular Pathway and Genes Associated with Heading Type in Chinese Cabbage
Article Snippet: Total RNA was isolated with an RNA extraction kit (Huayueyang, China), and quantified with a Qubit® RNA Assay Kit on the Qubit® 2.0 Fluorometer (Life Technologies, CA, USA). .. The RNA integrity was assessed on the Bioanalyzer 2100 System using the RNA Nano 6000 Assay Kit (Agilent).

Article Title: Deep RNA sequencing of pectoralis muscle transcriptomes during late-term embryonic to neonatal development in indigenous Chinese duck breeds
Article Snippet: Paragraph title: RNA isolation, library preparation, and sequencing ... RNA purity and integrity were checked using a Nano Photometer® spectrophotometer (Implen, Westlake Village, CA, USA) and an RNA Nano 6000 Assay Kit (Bioanalyzer 2100 system; Agilent Technologies, Palo Alto, CA, USA), respectively.

Article Title: Deep Circular RNA Sequencing Provides Insights into the Mechanism Underlying Grass Carp Reovirus Infection
Article Snippet: Paragraph title: 4.4. RNA Isolation, Library Construction, and Sequencing ... RNA concentration and integrity was measured using the Qubit RNA assay kit (Life Technologies, Carlsbad, CA, USA) and the RNA Nano 6000 assay kit (Agilent Technologies, Santa Clara, CA, USA), respectively.

Microscopy:

Article Title: Deep Illumina sequencing reveals differential expression of long non-coding RNAs in hyperoxia induced bronchopulmonary dysplasia in a rat model
Article Snippet: RNA concentration was measured using a microscope spectrophotometer (GeneQuant 1300). .. Agarose gel electrophoresis was applied, and RNA integrity was assessed with the RNA Nano 6000 assay kit (Agilent Technologies, USA).

Mouse Assay:

Article Title: RNA-Sequencing and Bioinformatics Analysis of Long Noncoding RNAs and mRNAs in the Prefrontal Cortex of Mice Following Repeated Social Defeat Stress
Article Snippet: For PFC tissue collection, eligible C57/BL6 mice were immediately decapitated after the EPM test. .. In addition, RNA integrity was assessed with RNA Nano 6000 Assay Kit (Agilent Technologies, Foster City, CA, USA).

Polymerase Chain Reaction:

Article Title: Molecular mechanisms of tannin accumulation in Rhus galls and genes involved in plant-insect interactions
Article Snippet: RNA purity and integrity were assessed using a RNA Nano 6000 Assay Kit (Agilent Technologies, CA, USA). .. First strand cDNA was synthesized with random hexamers as primers and mRNA fragments as templates.

Article Title: Application of RNA-seq for mitogenome reconstruction, and reconsideration of long-branch artifacts in Hemiptera phylogeny
Article Snippet: Finally, the cDNA fragments were enriched by PCR amplification using random hexamers, and products were purified by AMPure XP beads to generate final library. .. The size and purity of the cDNA sequencing libraries were determined using a 2100 Bioanalyzer with the RNA Nano 6000 Assay Kit (Agilent Technologies, CA, USA) and the quantity was estimated using Q-PCR.

Construct:

Article Title: Deep Illumina sequencing reveals differential expression of long non-coding RNAs in hyperoxia induced bronchopulmonary dysplasia in a rat model
Article Snippet: Agarose gel electrophoresis was applied, and RNA integrity was assessed with the RNA Nano 6000 assay kit (Agilent Technologies, USA). .. The 1 μg RNA from each sample was used according to the VAHTS Total RNA-seq (H/M/R) Library Prep Kit for Illumina® Kit.

cDNA Library Assay:

Article Title: Transcriptome analyses suggest a novel hypothesis for whitefly adaptation to tobacco
Article Snippet: The integrity of the RNA samples was analysed on the 2100 Bioanalyzer with RNA Nano 6000 Assay Kit (Agilent Technologies, USA). .. The integrity of the RNA samples was analysed on the 2100 Bioanalyzer with RNA Nano 6000 Assay Kit (Agilent Technologies, USA).

Agarose Gel Electrophoresis:

Article Title: Deep Illumina sequencing reveals differential expression of long non-coding RNAs in hyperoxia induced bronchopulmonary dysplasia in a rat model
Article Snippet: RNA concentration was measured using a microscope spectrophotometer (GeneQuant 1300). .. Agarose gel electrophoresis was applied, and RNA integrity was assessed with the RNA Nano 6000 assay kit (Agilent Technologies, USA). .. The 1 μg RNA from each sample was used according to the VAHTS Total RNA-seq (H/M/R) Library Prep Kit for Illumina® Kit.

Article Title: Molecular mechanisms of tannin accumulation in Rhus galls and genes involved in plant-insect interactions
Article Snippet: RNA purity and integrity were assessed using a RNA Nano 6000 Assay Kit (Agilent Technologies, CA, USA). .. The double-stranded cDNA was synthesized and purified using a QiaQuick PCR Extraction kit (QIAGEN, MD, USA) and washed using EB buffer for end repair and the addition of single adenines.

Article Title: Filtered reproductive long non-coding RNAs by genome-wide analyses of goat ovary at different estrus periods
Article Snippet: Then, 1% agarose gel electrophoresis was prepared to monitor the degradation and contamination of the extracted RNA. .. Finally, the RNA integrity was assessed by the RNA Nano 6000 assay kit (Agilent Technologies, CA, USA) with the Bioanalyzer 2100 system.

Article Title: Comparative transcriptome analysis of Rimicaris sp. reveals novel molecular features associated with survival in deep-sea hydrothermal vent
Article Snippet: RNA quality was examined by 1% agarose gel electrophoresis. .. RNA integrity was assessed by using a RNA Nano 6000 assay kit (Agilent Technologies, CA, USA).

Article Title: Identification and characterization of microRNAs in the pituitary of pubescent goats
Article Snippet: RNA integrity for sequencing was assessed using the RNA Nano 6000 Assay Kit and a Agilent Bioanalyzer 2100 system (Agilent Technologies, CA, USA). .. The concentration of total RNA for qRT-PCR was quantifiedc at 260 nm with a NanoDrop spectrophotometer (ND-2000, USA).

Purification:

Article Title: Transcriptome sequencing identifies key pathways and genes involved in gastric adenocarcinoma
Article Snippet: RNA integrity was detected using the RNA Nano 6000 Assay kit (Agilent Technologies, Inc., Santa Clara, CA, USA). .. RNA integrity was detected using the RNA Nano 6000 Assay kit (Agilent Technologies, Inc., Santa Clara, CA, USA).

Article Title: Molecular mechanisms of tannin accumulation in Rhus galls and genes involved in plant-insect interactions
Article Snippet: RNA purity and integrity were assessed using a RNA Nano 6000 Assay Kit (Agilent Technologies, CA, USA). .. First strand cDNA was synthesized with random hexamers as primers and mRNA fragments as templates.

Article Title: Comparative transcriptome analysis of Rimicaris sp. reveals novel molecular features associated with survival in deep-sea hydrothermal vent
Article Snippet: RNA integrity was assessed by using a RNA Nano 6000 assay kit (Agilent Technologies, CA, USA). .. RNA integrity was assessed by using a RNA Nano 6000 assay kit (Agilent Technologies, CA, USA).

Article Title: Application of RNA-seq for mitogenome reconstruction, and reconsideration of long-branch artifacts in Hemiptera phylogeny
Article Snippet: Finally, the cDNA fragments were enriched by PCR amplification using random hexamers, and products were purified by AMPure XP beads to generate final library. .. The size and purity of the cDNA sequencing libraries were determined using a 2100 Bioanalyzer with the RNA Nano 6000 Assay Kit (Agilent Technologies, CA, USA) and the quantity was estimated using Q-PCR.

Article Title: Deep RNA sequencing of pectoralis muscle transcriptomes during late-term embryonic to neonatal development in indigenous Chinese duck breeds
Article Snippet: RNA purity and integrity were checked using a Nano Photometer® spectrophotometer (Implen, Westlake Village, CA, USA) and an RNA Nano 6000 Assay Kit (Bioanalyzer 2100 system; Agilent Technologies, Palo Alto, CA, USA), respectively. .. Sequencing libraries were generated using an Illumina Tru Seq™ RNA sample preparation kit (Illumina, San Diego, CA, USA) following the manufacturer’s recommendations, and index codes were added to attribute a sequence to each sample.

Real-time Polymerase Chain Reaction:

Article Title: Deep Illumina sequencing reveals differential expression of long non-coding RNAs in hyperoxia induced bronchopulmonary dysplasia in a rat model
Article Snippet: Agarose gel electrophoresis was applied, and RNA integrity was assessed with the RNA Nano 6000 assay kit (Agilent Technologies, USA). .. The 1 μg RNA from each sample was used according to the VAHTS Total RNA-seq (H/M/R) Library Prep Kit for Illumina® Kit.

Article Title: Global and Complement Gene-Specific DNA Methylation in Grass Carp after Grass Carp Reovirus (GCRV) Infection
Article Snippet: Paragraph title: 4.5. RNA Isolation and Quantitative Real-Time PCR (RT-qPCR) ... The Qubit RNA assay kit (Life Technologies, Carlsbad, CA, USA) and RNA Nano 6000 assay kit (Agilent Technologies, Santa Clara, CA, USA) were used to measure the concentration and integrity of isolated RNA, respectively.

RNA Extraction:

Article Title: Molecular mechanisms of tannin accumulation in Rhus galls and genes involved in plant-insect interactions
Article Snippet: After washed three times in DEPC-treated (diethylpyrocarbonate) water, 100 mg gallnut tissues of each developmental stage were used to isolate total RNA separately using an RNA Extraction Kit (BioTeke Corporation, Beijing, China) according to the manufacturer’s protocols. .. RNA purity and integrity were assessed using a RNA Nano 6000 Assay Kit (Agilent Technologies, CA, USA).

Article Title: Filtered reproductive long non-coding RNAs by genome-wide analyses of goat ovary at different estrus periods
Article Snippet: Paragraph title: RNA extraction and library construction ... Finally, the RNA integrity was assessed by the RNA Nano 6000 assay kit (Agilent Technologies, CA, USA) with the Bioanalyzer 2100 system.

Article Title: Comparative transcriptome analysis of Rimicaris sp. reveals novel molecular features associated with survival in deep-sea hydrothermal vent
Article Snippet: Paragraph title: RNA extraction and library preparation ... RNA integrity was assessed by using a RNA Nano 6000 assay kit (Agilent Technologies, CA, USA).

Article Title: Transcriptome profiling of cancer tissues in Chinese patients with gastric cancer by high-throughput sequencing
Article Snippet: Paragraph title: Tissue sample preparation and RNA extraction ... The quantity and quality (including the ratio of 28S/18S and RNA integrity number) of each RNA sample was assessed using the RNA Nano 6000 assay kit (Agilent Technologies, Inc., Santa Clara, CA, USA) and Bioanalyzer 2100 system (Agilent Technologies, Inc.).

Article Title: Coupling Seq-BSA and RNA-Seq Analyses Reveal the Molecular Pathway and Genes Associated with Heading Type in Chinese Cabbage
Article Snippet: Paragraph title: RNA extraction and quality test ... The RNA integrity was assessed on the Bioanalyzer 2100 System using the RNA Nano 6000 Assay Kit (Agilent).

Selection:

Article Title: Application of RNA-seq for mitogenome reconstruction, and reconsideration of long-branch artifacts in Hemiptera phylogeny
Article Snippet: Size selection was performed using AMPure XP beads. .. The size and purity of the cDNA sequencing libraries were determined using a 2100 Bioanalyzer with the RNA Nano 6000 Assay Kit (Agilent Technologies, CA, USA) and the quantity was estimated using Q-PCR.

Sample Prep:

Article Title: Transcriptome analyses suggest a novel hypothesis for whitefly adaptation to tobacco
Article Snippet: Paragraph title: Sample preparation and Illumina sequencing ... The integrity of the RNA samples was analysed on the 2100 Bioanalyzer with RNA Nano 6000 Assay Kit (Agilent Technologies, USA).

Article Title: Transcriptome profiling of cancer tissues in Chinese patients with gastric cancer by high-throughput sequencing
Article Snippet: Paragraph title: Tissue sample preparation and RNA extraction ... The quantity and quality (including the ratio of 28S/18S and RNA integrity number) of each RNA sample was assessed using the RNA Nano 6000 assay kit (Agilent Technologies, Inc., Santa Clara, CA, USA) and Bioanalyzer 2100 system (Agilent Technologies, Inc.).

Article Title: Deep RNA sequencing of pectoralis muscle transcriptomes during late-term embryonic to neonatal development in indigenous Chinese duck breeds
Article Snippet: RNA purity and integrity were checked using a Nano Photometer® spectrophotometer (Implen, Westlake Village, CA, USA) and an RNA Nano 6000 Assay Kit (Bioanalyzer 2100 system; Agilent Technologies, Palo Alto, CA, USA), respectively. .. RNA purity and integrity were checked using a Nano Photometer® spectrophotometer (Implen, Westlake Village, CA, USA) and an RNA Nano 6000 Assay Kit (Bioanalyzer 2100 system; Agilent Technologies, Palo Alto, CA, USA), respectively.

Article Title: Deep Circular RNA Sequencing Provides Insights into the Mechanism Underlying Grass Carp Reovirus Infection
Article Snippet: RNA concentration and integrity was measured using the Qubit RNA assay kit (Life Technologies, Carlsbad, CA, USA) and the RNA Nano 6000 assay kit (Agilent Technologies, Santa Clara, CA, USA), respectively. .. RNA concentration and integrity was measured using the Qubit RNA assay kit (Life Technologies, Carlsbad, CA, USA) and the RNA Nano 6000 assay kit (Agilent Technologies, Santa Clara, CA, USA), respectively.

Ethanol Precipitation:

Article Title: Filtered reproductive long non-coding RNAs by genome-wide analyses of goat ovary at different estrus periods
Article Snippet: Finally, the RNA integrity was assessed by the RNA Nano 6000 assay kit (Agilent Technologies, CA, USA) with the Bioanalyzer 2100 system. .. Finally, the RNA integrity was assessed by the RNA Nano 6000 assay kit (Agilent Technologies, CA, USA) with the Bioanalyzer 2100 system.

Spectrophotometry:

Article Title: RNA-Sequencing and Bioinformatics Analysis of Long Noncoding RNAs and mRNAs in the Prefrontal Cortex of Mice Following Repeated Social Defeat Stress
Article Snippet: RNA purity was assessed on a NanoPhotometer® spectrophotometer (IMPLEN, CA, USA) and the concentration was measured with Qubit® RNA Assay Kit on a Qubit® 2.0 fluorometer (Life Technologies, CA, USA) according to the manufacturer's instructions. .. In addition, RNA integrity was assessed with RNA Nano 6000 Assay Kit (Agilent Technologies, Foster City, CA, USA).

Article Title: Deep Illumina sequencing reveals differential expression of long non-coding RNAs in hyperoxia induced bronchopulmonary dysplasia in a rat model
Article Snippet: RNA concentration was measured using a microscope spectrophotometer (GeneQuant 1300). .. Agarose gel electrophoresis was applied, and RNA integrity was assessed with the RNA Nano 6000 assay kit (Agilent Technologies, USA).

Article Title: Filtered reproductive long non-coding RNAs by genome-wide analyses of goat ovary at different estrus periods
Article Snippet: The purity and concentration of the RNA were respectively measured by the NanoPhotometer® spectrophotometer (IMPLEN, CA, USA) and the Qubit ® 2.0 Fluorometer (Life Technologies, CA, USA). .. Finally, the RNA integrity was assessed by the RNA Nano 6000 assay kit (Agilent Technologies, CA, USA) with the Bioanalyzer 2100 system.

Article Title: Functional Characterization and Evolutionary Analysis of Glycine-Betaine Biosynthesis Pathway in Red Seaweed Pyropia yezoensis
Article Snippet: Purity was evaluated using a NanoPhotometer spectrophotometer (IMPLEN, CA, U.S.). .. RNA integrity was assessed using an RNA Nano 6000 Assay Kit and the Bioanalyzer 2100 system (Agilent Technologies, CA, U.S.).

Article Title: Transcriptomic Analysis of Coding Genes and Non-Coding RNAs Reveals Complex Regulatory Networks Underlying the Black Back and White Belly Coat Phenotype in Chinese Wuzhishan Pigs
Article Snippet: RNA purity was determined using a K5500 Spectrophotometer (Kaiao, Beijing, China). .. RNA integrity and concentration was assessed using the RNA Nano 6000 Assay Kit and the Bioanalyzer 2100 system (Agilent Technologies, Foster, CA, USA).

Article Title: Deep RNA sequencing of pectoralis muscle transcriptomes during late-term embryonic to neonatal development in indigenous Chinese duck breeds
Article Snippet: RNA concentration was measured using a Qubit® RNA Assay Kit in a Qubit® 2.0 Fluorometer (Life Technologies, CA, USA). .. RNA purity and integrity were checked using a Nano Photometer® spectrophotometer (Implen, Westlake Village, CA, USA) and an RNA Nano 6000 Assay Kit (Bioanalyzer 2100 system; Agilent Technologies, Palo Alto, CA, USA), respectively. .. A total amount of 3 μg RNA was used as input material for the RNA sample preparations.

Concentration Assay:

Article Title: RNA-Sequencing and Bioinformatics Analysis of Long Noncoding RNAs and mRNAs in the Prefrontal Cortex of Mice Following Repeated Social Defeat Stress
Article Snippet: RNA purity was assessed on a NanoPhotometer® spectrophotometer (IMPLEN, CA, USA) and the concentration was measured with Qubit® RNA Assay Kit on a Qubit® 2.0 fluorometer (Life Technologies, CA, USA) according to the manufacturer's instructions. .. In addition, RNA integrity was assessed with RNA Nano 6000 Assay Kit (Agilent Technologies, Foster City, CA, USA).

Article Title: Deep Illumina sequencing reveals differential expression of long non-coding RNAs in hyperoxia induced bronchopulmonary dysplasia in a rat model
Article Snippet: RNA concentration was measured using a microscope spectrophotometer (GeneQuant 1300). .. Agarose gel electrophoresis was applied, and RNA integrity was assessed with the RNA Nano 6000 assay kit (Agilent Technologies, USA).

Article Title: Filtered reproductive long non-coding RNAs by genome-wide analyses of goat ovary at different estrus periods
Article Snippet: The purity and concentration of the RNA were respectively measured by the NanoPhotometer® spectrophotometer (IMPLEN, CA, USA) and the Qubit ® 2.0 Fluorometer (Life Technologies, CA, USA). .. Finally, the RNA integrity was assessed by the RNA Nano 6000 assay kit (Agilent Technologies, CA, USA) with the Bioanalyzer 2100 system.

Article Title: Functional Characterization and Evolutionary Analysis of Glycine-Betaine Biosynthesis Pathway in Red Seaweed Pyropia yezoensis
Article Snippet: RNA concentration was measured using a Qubit RNA Assay Kit and Qubit 2.0 Fluorometer (Life Technologies, CA, U.S.). .. RNA integrity was assessed using an RNA Nano 6000 Assay Kit and the Bioanalyzer 2100 system (Agilent Technologies, CA, U.S.).

Article Title: Transcriptomic Analysis of Coding Genes and Non-Coding RNAs Reveals Complex Regulatory Networks Underlying the Black Back and White Belly Coat Phenotype in Chinese Wuzhishan Pigs
Article Snippet: RNA purity was determined using a K5500 Spectrophotometer (Kaiao, Beijing, China). .. RNA integrity and concentration was assessed using the RNA Nano 6000 Assay Kit and the Bioanalyzer 2100 system (Agilent Technologies, Foster, CA, USA). .. Sequencing libraries were prepared using 3 μg of RNA per sample.

Article Title: Transcriptome analysis of the salivary glands of the grain aphid, Sitobion avenae
Article Snippet: RNA concentration was measured using Qubit RNA Assay Kit and Qubit 2.0 Flurometer (Life Technologies, CA, USA). .. RNA integrity was assessed using the RNA Nano 6000 Assay Kit and the Bioanalyzer 2100 system (Agilent Technologies, CA, USA).

Article Title: Comparative transcriptome analysis of Rimicaris sp. reveals novel molecular features associated with survival in deep-sea hydrothermal vent
Article Snippet: RNA concentration was measured using a Qubit® RNA assay kit in a Qubit® 2.0 Fluorometer (Life Technologies, CA, USA). .. RNA integrity was assessed by using a RNA Nano 6000 assay kit (Agilent Technologies, CA, USA).

Article Title: Global and Complement Gene-Specific DNA Methylation in Grass Carp after Grass Carp Reovirus (GCRV) Infection
Article Snippet: TRizol reagent (Invitrogen, Carlsbad, CA, USA) was used to isolate RNA from spleen samples according the manufacturer’s protocol. .. The Qubit RNA assay kit (Life Technologies, Carlsbad, CA, USA) and RNA Nano 6000 assay kit (Agilent Technologies, Santa Clara, CA, USA) were used to measure the concentration and integrity of isolated RNA, respectively. .. DNase I (Promega, Madison, WI, USA)-treated total RNA was used as the template for the first-strand cDNA synthesis.

Article Title: Deep RNA sequencing of pectoralis muscle transcriptomes during late-term embryonic to neonatal development in indigenous Chinese duck breeds
Article Snippet: RNA concentration was measured using a Qubit® RNA Assay Kit in a Qubit® 2.0 Fluorometer (Life Technologies, CA, USA). .. RNA purity and integrity were checked using a Nano Photometer® spectrophotometer (Implen, Westlake Village, CA, USA) and an RNA Nano 6000 Assay Kit (Bioanalyzer 2100 system; Agilent Technologies, Palo Alto, CA, USA), respectively.

Article Title: Deep Circular RNA Sequencing Provides Insights into the Mechanism Underlying Grass Carp Reovirus Infection
Article Snippet: The isolated RNA was further treated by RNase-Free DNase (Promega, Madison, WI, USA) in order to remove possible genomic DNA. .. RNA concentration and integrity was measured using the Qubit RNA assay kit (Life Technologies, Carlsbad, CA, USA) and the RNA Nano 6000 assay kit (Agilent Technologies, Santa Clara, CA, USA), respectively. .. High quality RNA was used for further library construction.

Staining:

Article Title: Transcriptomic Analysis of Coding Genes and Non-Coding RNAs Reveals Complex Regulatory Networks Underlying the Black Back and White Belly Coat Phenotype in Chinese Wuzhishan Pigs
Article Snippet: The white and black skin were found to have similar cellular composition and the melanocytes were identified in white skin by DOPA (3,4-Dihydroxy-L-phenylalanine) staining ( ). .. RNA integrity and concentration was assessed using the RNA Nano 6000 Assay Kit and the Bioanalyzer 2100 system (Agilent Technologies, Foster, CA, USA).

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    Agilent technologies rna 6000 nano kit
    Over-drying time does not affect quantity of small RNA in mirRICH method. (A) 15% PAGE (Polyacrylamide gel electrophoresis) and (B) 15% UREA denaturing gels electrophoresis of the RNA samples are prepared from two different breast cancer cells, MDA-MB-231 and MCF7 cells with either TRIzol with 1hr drying or 24hr drying, mirRICH with 1hr drying or 24hr drying. The 100-bp marker is indicated as ladder. (C) Peak images of experiment samples of MDA-MB-231 and MCF7 were obtained by Aglient RNA 6000 <t>Nano</t> kit respectively.
    Rna 6000 Nano Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 392 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies bioanalyzer rna nano 6000 kit
    <t>Bioanalyzer</t> profiles of the large <t>RNA</t> fractions isolated from donors with the lowest and highest blood RNA level. The large RNA fractions (200 ng) from the low RNA level sample 180 (6.7 μg RNA/ml blood) and the high RNA level sample 162 (22.7 μg RNA/ml blood) were separated using the Bioanalyzer RNA Nano 6000 Kit. Shown are positions of: the (18S) and (28S) ribosomal RNA; the 600 nt globin region (G); and Bioanalyzer marker (M).
    Bioanalyzer Rna Nano 6000 Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 78/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Agilent technologies bioanalyser
    Microsampling protocol design and reproducibility. a In terminal blood sampling, at each time point, groups of mice are exsanguinated to obtain 0.5–0.6 mL blood volumes, which are then subject to leukocyte depletion and saponin lysis before TRIzol treatment. Thus, the number of mice increases proportionally with the number of biological replicates and time points in the study design (number of mice per timepoint X number of timepoints; NT). Microsampling involves obtaining sample volumes as low as 20 μL from the same mouse at different time points, thus confining the number of mice to just biological replicates (N) and significantly lowering costs and biological variability. Leukocyte depletion and saponin lysis are also not performed on the low volume samples, thus saving time and manpower. b Heatmap shows pair-wise Pearson correlation coefficients and the inset shows multidimensional scaling to visualize the level of similarity between the P. vinckei microsamples. Microsamples show low degree of variability and are highly reproducible as proved by tight correlations between biological replicates. c High Pearson correlations were observed between normalized gene expression values (shown as logarithm of fragments per kilobase of transcript per million mapped reads) from microsampling (x-axis) and terminal blood sampling (y-axis) methods. d <t>Bioanalyser</t> electrophoregrams of total RNA from Plasmodium vinckei vinckei CY microsamples show that high quality RNA could be extracted consistently from 20 μL microsamples
    Bioanalyser, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 122 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Over-drying time does not affect quantity of small RNA in mirRICH method. (A) 15% PAGE (Polyacrylamide gel electrophoresis) and (B) 15% UREA denaturing gels electrophoresis of the RNA samples are prepared from two different breast cancer cells, MDA-MB-231 and MCF7 cells with either TRIzol with 1hr drying or 24hr drying, mirRICH with 1hr drying or 24hr drying. The 100-bp marker is indicated as ladder. (C) Peak images of experiment samples of MDA-MB-231 and MCF7 were obtained by Aglient RNA 6000 Nano kit respectively.

    Journal: RNA Biology

    Article Title: mirRICH, a simple method to enrich the small RNA fraction from over-dried RNA pellets

    doi: 10.1080/15476286.2018.1451723

    Figure Lengend Snippet: Over-drying time does not affect quantity of small RNA in mirRICH method. (A) 15% PAGE (Polyacrylamide gel electrophoresis) and (B) 15% UREA denaturing gels electrophoresis of the RNA samples are prepared from two different breast cancer cells, MDA-MB-231 and MCF7 cells with either TRIzol with 1hr drying or 24hr drying, mirRICH with 1hr drying or 24hr drying. The 100-bp marker is indicated as ladder. (C) Peak images of experiment samples of MDA-MB-231 and MCF7 were obtained by Aglient RNA 6000 Nano kit respectively.

    Article Snippet: Agilent RNA 6000 Nano kit and small RNA analysis kit was purchased from Agilent.

    Techniques: Polyacrylamide Gel Electrophoresis, Electrophoresis, Multiple Displacement Amplification, Marker

    Comparison of the quantity and the quality of small RNAs isolated by TRIzol, mirRICH and mirVana. Equal number of two different breast cancer cells, MDA-MB-231 and MCF7 cells were prepared and extracted with three different methods: TRIzol, mirRICH, and mirVana. Peak images of small RNA samples were obtained by Aglient RNA 6000 Nano kit (A) and small RNA analysis kit (B) respectively. (C) 15% PAGE (Polyacrylamide gel electrophoresis) and (D) 15% UREA denaturing gels electrophoresis of the RNA samples from two different breast cancer cells, MDA-MB-231 and MCF7 are prepared by three different methods, TRIzol (T), mirRICH (M) and mirVana (K). The 100-bp marker is indicated as ladder.

    Journal: RNA Biology

    Article Title: mirRICH, a simple method to enrich the small RNA fraction from over-dried RNA pellets

    doi: 10.1080/15476286.2018.1451723

    Figure Lengend Snippet: Comparison of the quantity and the quality of small RNAs isolated by TRIzol, mirRICH and mirVana. Equal number of two different breast cancer cells, MDA-MB-231 and MCF7 cells were prepared and extracted with three different methods: TRIzol, mirRICH, and mirVana. Peak images of small RNA samples were obtained by Aglient RNA 6000 Nano kit (A) and small RNA analysis kit (B) respectively. (C) 15% PAGE (Polyacrylamide gel electrophoresis) and (D) 15% UREA denaturing gels electrophoresis of the RNA samples from two different breast cancer cells, MDA-MB-231 and MCF7 are prepared by three different methods, TRIzol (T), mirRICH (M) and mirVana (K). The 100-bp marker is indicated as ladder.

    Article Snippet: Agilent RNA 6000 Nano kit and small RNA analysis kit was purchased from Agilent.

    Techniques: Isolation, Multiple Displacement Amplification, Polyacrylamide Gel Electrophoresis, Electrophoresis, Marker

    Bioanalyzer profiles of the large RNA fractions isolated from donors with the lowest and highest blood RNA level. The large RNA fractions (200 ng) from the low RNA level sample 180 (6.7 μg RNA/ml blood) and the high RNA level sample 162 (22.7 μg RNA/ml blood) were separated using the Bioanalyzer RNA Nano 6000 Kit. Shown are positions of: the (18S) and (28S) ribosomal RNA; the 600 nt globin region (G); and Bioanalyzer marker (M).

    Journal: PLoS ONE

    Article Title: Inter-Individual Differences in RNA Levels in Human Peripheral Blood

    doi: 10.1371/journal.pone.0148260

    Figure Lengend Snippet: Bioanalyzer profiles of the large RNA fractions isolated from donors with the lowest and highest blood RNA level. The large RNA fractions (200 ng) from the low RNA level sample 180 (6.7 μg RNA/ml blood) and the high RNA level sample 162 (22.7 μg RNA/ml blood) were separated using the Bioanalyzer RNA Nano 6000 Kit. Shown are positions of: the (18S) and (28S) ribosomal RNA; the 600 nt globin region (G); and Bioanalyzer marker (M).

    Article Snippet: The isolated RNA samples were analyzed with the Agilent Bioanalyzer RNA Nano 6000 Kit (Agilent Technologies, Inc., Santa Clara, CA).

    Techniques: Isolation, Marker

    Characteristics of RNA isolated from healthy donors. a) Proportion of the large and small RNA fractions in relation to increasing quantities of total RNA in human peripheral blood. Samples of RNA from the 35 individuals are sequentially ranked in accord with the increasing amount of total RNA in the sample. For each sample, the amounts of the large RNA fraction (inverted triangle) and small RNA fraction (square) are depicted. b) The large RNA and small RNA fractions are expressed as a percentage of the total RNA in the sample. c) Bioanalyzer RIN values of the large RNA fractions from 35 samples.

    Journal: PLoS ONE

    Article Title: Inter-Individual Differences in RNA Levels in Human Peripheral Blood

    doi: 10.1371/journal.pone.0148260

    Figure Lengend Snippet: Characteristics of RNA isolated from healthy donors. a) Proportion of the large and small RNA fractions in relation to increasing quantities of total RNA in human peripheral blood. Samples of RNA from the 35 individuals are sequentially ranked in accord with the increasing amount of total RNA in the sample. For each sample, the amounts of the large RNA fraction (inverted triangle) and small RNA fraction (square) are depicted. b) The large RNA and small RNA fractions are expressed as a percentage of the total RNA in the sample. c) Bioanalyzer RIN values of the large RNA fractions from 35 samples.

    Article Snippet: The isolated RNA samples were analyzed with the Agilent Bioanalyzer RNA Nano 6000 Kit (Agilent Technologies, Inc., Santa Clara, CA).

    Techniques: Isolation

    Microsampling protocol design and reproducibility. a In terminal blood sampling, at each time point, groups of mice are exsanguinated to obtain 0.5–0.6 mL blood volumes, which are then subject to leukocyte depletion and saponin lysis before TRIzol treatment. Thus, the number of mice increases proportionally with the number of biological replicates and time points in the study design (number of mice per timepoint X number of timepoints; NT). Microsampling involves obtaining sample volumes as low as 20 μL from the same mouse at different time points, thus confining the number of mice to just biological replicates (N) and significantly lowering costs and biological variability. Leukocyte depletion and saponin lysis are also not performed on the low volume samples, thus saving time and manpower. b Heatmap shows pair-wise Pearson correlation coefficients and the inset shows multidimensional scaling to visualize the level of similarity between the P. vinckei microsamples. Microsamples show low degree of variability and are highly reproducible as proved by tight correlations between biological replicates. c High Pearson correlations were observed between normalized gene expression values (shown as logarithm of fragments per kilobase of transcript per million mapped reads) from microsampling (x-axis) and terminal blood sampling (y-axis) methods. d Bioanalyser electrophoregrams of total RNA from Plasmodium vinckei vinckei CY microsamples show that high quality RNA could be extracted consistently from 20 μL microsamples

    Journal: Malaria Journal

    Article Title: A fast and cost-effective microsampling protocol incorporating reduced animal usage for time-series transcriptomics in rodent malaria parasites

    doi: 10.1186/s12936-019-2659-4

    Figure Lengend Snippet: Microsampling protocol design and reproducibility. a In terminal blood sampling, at each time point, groups of mice are exsanguinated to obtain 0.5–0.6 mL blood volumes, which are then subject to leukocyte depletion and saponin lysis before TRIzol treatment. Thus, the number of mice increases proportionally with the number of biological replicates and time points in the study design (number of mice per timepoint X number of timepoints; NT). Microsampling involves obtaining sample volumes as low as 20 μL from the same mouse at different time points, thus confining the number of mice to just biological replicates (N) and significantly lowering costs and biological variability. Leukocyte depletion and saponin lysis are also not performed on the low volume samples, thus saving time and manpower. b Heatmap shows pair-wise Pearson correlation coefficients and the inset shows multidimensional scaling to visualize the level of similarity between the P. vinckei microsamples. Microsamples show low degree of variability and are highly reproducible as proved by tight correlations between biological replicates. c High Pearson correlations were observed between normalized gene expression values (shown as logarithm of fragments per kilobase of transcript per million mapped reads) from microsampling (x-axis) and terminal blood sampling (y-axis) methods. d Bioanalyser electrophoregrams of total RNA from Plasmodium vinckei vinckei CY microsamples show that high quality RNA could be extracted consistently from 20 μL microsamples

    Article Snippet: The RNA pellet was resuspended in 15 μL nuclease-free water, RNA quantity measured by Qubit fluorometer and RNA integrity measured by Agilent Bioanalyser (Agilent RNA 6000 Nano kit Cat#5067-1511).

    Techniques: Sampling, Mouse Assay, Lysis, Expressing