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Agilent technologies rna nano 6000 assay kit
Rna Nano 6000 Assay Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 93/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rna nano 6000 assay kit/product/Agilent technologies
Average 93 stars, based on 28 article reviews
Price from $9.99 to $1999.99
rna nano 6000 assay kit - by Bioz Stars, 2020-07
93/100 stars

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Concentration Assay:

Article Title: Transcriptomic analysis of gills provides insights into the molecular basis of molting in Chinese mitten crab (Eriocheir sinensis)
Article Snippet: .. The concentration and integrity of the total RNA were assessed using the Qubit® RNA Assay Kit with a Qubit® 2.0 fluorometer (Life Technologies, CA, USA) and the RNA Nano 6000 Assay Kit for the Agilent Bioanalyzer 2100 system (Agilent Technologies, CA, USA). .. Sequencing libraries were prepared using the NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, Ipswich, MA, USA) following the manufacturer’s recommendations, and index codes were added to tag the sequences.

Article Title: Brassica yellows virus’ movement protein upregulates anthocyanin accumulation, leading to the development of purple leaf symptoms on Arabidopsis thaliana
Article Snippet: .. For RNA-seq the concentration and integrity of the total RNA was further assessed by Beijing Novogene Bioinformatics Technology Co. Ltd. (BNBTC), Beijing, China, using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies). .. Genomic DNA of Arabidopsis wild type (Col-0) and transgenic lines were isolated from the rosette leaves of 6-week-old Arabidopsis seedlings using a CTAB procedure .

RNA Sequencing Assay:

Article Title: Brassica yellows virus’ movement protein upregulates anthocyanin accumulation, leading to the development of purple leaf symptoms on Arabidopsis thaliana
Article Snippet: .. For RNA-seq the concentration and integrity of the total RNA was further assessed by Beijing Novogene Bioinformatics Technology Co. Ltd. (BNBTC), Beijing, China, using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies). .. Genomic DNA of Arabidopsis wild type (Col-0) and transgenic lines were isolated from the rosette leaves of 6-week-old Arabidopsis seedlings using a CTAB procedure .

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    Agilent technologies rna 6000 nano kit
    RNA quality of cultivated tissue slices. RNA quality was determined by a Bioanalyzer 2100 using the <t>RNA</t> 6000 <t>Nano-Kit</t> (Agilent Technologies) and revealed good quality before the DNase digestion was performed ( a ). After the DNase digestion, the RNA quality was strongly reduced ( b ). The left graphs show untreated peritumoral brain tissue, the right graphs the corresponding GBM tissue.
    Rna 6000 Nano Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 1508 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rna 6000 nano kit/product/Agilent technologies
    Average 92 stars, based on 1508 article reviews
    Price from $9.99 to $1999.99
    rna 6000 nano kit - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    90
    Agilent technologies bioanalyzer rna nano 6000 kit
    <t>Bioanalyzer</t> profiles of the large <t>RNA</t> fractions isolated from donors with the lowest and highest blood RNA level. The large RNA fractions (200 ng) from the low RNA level sample 180 (6.7 μg RNA/ml blood) and the high RNA level sample 162 (22.7 μg RNA/ml blood) were separated using the Bioanalyzer RNA <t>Nano</t> 6000 Kit. Shown are positions of: the (18S) and (28S) ribosomal RNA; the 600 nt globin region (G); and Bioanalyzer marker (M).
    Bioanalyzer Rna Nano 6000 Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bioanalyzer rna nano 6000 kit/product/Agilent technologies
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bioanalyzer rna nano 6000 kit - by Bioz Stars, 2020-07
    90/100 stars
      Buy from Supplier

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    RNA quality of cultivated tissue slices. RNA quality was determined by a Bioanalyzer 2100 using the RNA 6000 Nano-Kit (Agilent Technologies) and revealed good quality before the DNase digestion was performed ( a ). After the DNase digestion, the RNA quality was strongly reduced ( b ). The left graphs show untreated peritumoral brain tissue, the right graphs the corresponding GBM tissue.

    Journal: Scientific Reports

    Article Title: Deep sequencing and automated histochemistry of human tissue slice cultures improve their usability as preclinical model for cancer research

    doi: 10.1038/s41598-019-56509-5

    Figure Lengend Snippet: RNA quality of cultivated tissue slices. RNA quality was determined by a Bioanalyzer 2100 using the RNA 6000 Nano-Kit (Agilent Technologies) and revealed good quality before the DNase digestion was performed ( a ). After the DNase digestion, the RNA quality was strongly reduced ( b ). The left graphs show untreated peritumoral brain tissue, the right graphs the corresponding GBM tissue.

    Article Snippet: RNA quality was determined by the Bioanalyzer 2100 using the RNA 6000 Nano-Kit (Agilent Technologies).

    Techniques:

    Transcription levels of putative genes involved in conidiation and sexual potency. Total RNAs were extracted from 8 different experimental conditions as indicated. The quality of extracted RNA samples was further analyzed with the RNA 6000 Nano kit by Agilent 2100 Bioanalyzer (see Materials and Methods). ( A ) Northern blots analysis of act1 (actin) and env1 transcription. The denaturing RNA agarose gel was stained with ethidium bromide, the 18S rRNA and 28S rRNA bands were clearly visible in the intact RNA samples. ( B–H ) qRT-PCR. Relative transcript abundance of representative genes in sexually potent and impotent conditions. Data were given as relative quantitative (RQ) values to one of the eight conditions as indicated. The transcripts of the ribosome protein gene rpl6e were used for normalization of the qRT-PCR data [43] .

    Journal: PLoS ONE

    Article Title: Blue Light Acts as a Double-Edged Sword in Regulating Sexual Development of Hypocrea jecorina (Trichoderma reesei)

    doi: 10.1371/journal.pone.0044969

    Figure Lengend Snippet: Transcription levels of putative genes involved in conidiation and sexual potency. Total RNAs were extracted from 8 different experimental conditions as indicated. The quality of extracted RNA samples was further analyzed with the RNA 6000 Nano kit by Agilent 2100 Bioanalyzer (see Materials and Methods). ( A ) Northern blots analysis of act1 (actin) and env1 transcription. The denaturing RNA agarose gel was stained with ethidium bromide, the 18S rRNA and 28S rRNA bands were clearly visible in the intact RNA samples. ( B–H ) qRT-PCR. Relative transcript abundance of representative genes in sexually potent and impotent conditions. Data were given as relative quantitative (RQ) values to one of the eight conditions as indicated. The transcripts of the ribosome protein gene rpl6e were used for normalization of the qRT-PCR data [43] .

    Article Snippet: The quality of extracted RNA was further analyzed with the RNA 6000 Nano kit by Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA).

    Techniques: Northern Blot, Agarose Gel Electrophoresis, Staining, Quantitative RT-PCR

    RNA integrity, protein levels and degradation, and cells size of stationary phase cells ( A–C, E–F ) Cell cultures at early stationary phase (t=6 h) were treated with 1mM KCN or transferred to an anaerobic chamber. At t=24 h, cells were pelleted for RNA, protein, and microscope analyses. For controls, untreated overnight cultures (t=24 h) and early stationary phase cultures (t=6 h) were used. ( A–B ) RNA quality was determined with a bioanalyzer using an RNA 6000 Nano kit. The degradation of rRNA was assessed with RNA integrity values which range from 10 (intact) to 1 (totally degraded). (C) Cells were sonicated and the protein content in the supernatant was determined with Bradford assays. (D) Before KCN treatment at t=6 h, the inducer for gfp expression was removed in the cultures with the cells carrying pQE-80L gfp-ssrA . After the KCN treatment, GFP levels were measured. Background fluorescence was determined using cells with empty vectors. (E–F) Phase contrast images of fixed cells were taken using a microscope, and cell size (fold change relative to 24 h untreated overnight cultures) were determined with ImageJ. (G–H) KCN treatment was performed at t=9 h. At t=24 h, microscope images were taken, and ampicillin and ofloxacin persister levels were determined. * signifies significant differences for comparisons to control groups (p-value

    Journal: Nature communications

    Article Title: Inhibition of stationary phase respiration impairs persister formation in E. coli

    doi: 10.1038/ncomms8983

    Figure Lengend Snippet: RNA integrity, protein levels and degradation, and cells size of stationary phase cells ( A–C, E–F ) Cell cultures at early stationary phase (t=6 h) were treated with 1mM KCN or transferred to an anaerobic chamber. At t=24 h, cells were pelleted for RNA, protein, and microscope analyses. For controls, untreated overnight cultures (t=24 h) and early stationary phase cultures (t=6 h) were used. ( A–B ) RNA quality was determined with a bioanalyzer using an RNA 6000 Nano kit. The degradation of rRNA was assessed with RNA integrity values which range from 10 (intact) to 1 (totally degraded). (C) Cells were sonicated and the protein content in the supernatant was determined with Bradford assays. (D) Before KCN treatment at t=6 h, the inducer for gfp expression was removed in the cultures with the cells carrying pQE-80L gfp-ssrA . After the KCN treatment, GFP levels were measured. Background fluorescence was determined using cells with empty vectors. (E–F) Phase contrast images of fixed cells were taken using a microscope, and cell size (fold change relative to 24 h untreated overnight cultures) were determined with ImageJ. (G–H) KCN treatment was performed at t=9 h. At t=24 h, microscope images were taken, and ampicillin and ofloxacin persister levels were determined. * signifies significant differences for comparisons to control groups (p-value

    Article Snippet: Quality of total RNA was assessed with a bioanalyzer using an RNA 6000 Nano kit (Agilent Technologies, Inc, Santa Clara, CA).

    Techniques: Microscopy, Sonication, Expressing, Fluorescence, Significance Assay

    Bioanalyzer profiles of the large RNA fractions isolated from donors with the lowest and highest blood RNA level. The large RNA fractions (200 ng) from the low RNA level sample 180 (6.7 μg RNA/ml blood) and the high RNA level sample 162 (22.7 μg RNA/ml blood) were separated using the Bioanalyzer RNA Nano 6000 Kit. Shown are positions of: the (18S) and (28S) ribosomal RNA; the 600 nt globin region (G); and Bioanalyzer marker (M).

    Journal: PLoS ONE

    Article Title: Inter-Individual Differences in RNA Levels in Human Peripheral Blood

    doi: 10.1371/journal.pone.0148260

    Figure Lengend Snippet: Bioanalyzer profiles of the large RNA fractions isolated from donors with the lowest and highest blood RNA level. The large RNA fractions (200 ng) from the low RNA level sample 180 (6.7 μg RNA/ml blood) and the high RNA level sample 162 (22.7 μg RNA/ml blood) were separated using the Bioanalyzer RNA Nano 6000 Kit. Shown are positions of: the (18S) and (28S) ribosomal RNA; the 600 nt globin region (G); and Bioanalyzer marker (M).

    Article Snippet: The isolated RNA samples were analyzed with the Agilent Bioanalyzer RNA Nano 6000 Kit (Agilent Technologies, Inc., Santa Clara, CA).

    Techniques: Isolation, Marker

    Characteristics of RNA isolated from healthy donors. a) Proportion of the large and small RNA fractions in relation to increasing quantities of total RNA in human peripheral blood. Samples of RNA from the 35 individuals are sequentially ranked in accord with the increasing amount of total RNA in the sample. For each sample, the amounts of the large RNA fraction (inverted triangle) and small RNA fraction (square) are depicted. b) The large RNA and small RNA fractions are expressed as a percentage of the total RNA in the sample. c) Bioanalyzer RIN values of the large RNA fractions from 35 samples.

    Journal: PLoS ONE

    Article Title: Inter-Individual Differences in RNA Levels in Human Peripheral Blood

    doi: 10.1371/journal.pone.0148260

    Figure Lengend Snippet: Characteristics of RNA isolated from healthy donors. a) Proportion of the large and small RNA fractions in relation to increasing quantities of total RNA in human peripheral blood. Samples of RNA from the 35 individuals are sequentially ranked in accord with the increasing amount of total RNA in the sample. For each sample, the amounts of the large RNA fraction (inverted triangle) and small RNA fraction (square) are depicted. b) The large RNA and small RNA fractions are expressed as a percentage of the total RNA in the sample. c) Bioanalyzer RIN values of the large RNA fractions from 35 samples.

    Article Snippet: The isolated RNA samples were analyzed with the Agilent Bioanalyzer RNA Nano 6000 Kit (Agilent Technologies, Inc., Santa Clara, CA).

    Techniques: Isolation