rna library prep kit  (New England Biolabs)


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  • 99
    Name:
    NEBNext Ultra RNA Library Prep Kit for Illumina
    Description:
    NEBNext Ultra RNA Library Prep Kit for Illumina 96 rxns
    Catalog Number:
    e7530l
    Price:
    3494
    Size:
    96 rxns
    Category:
    mRNA Template Preparation for PCR
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    Structured Review

    New England Biolabs rna library prep kit
    NEBNext Ultra RNA Library Prep Kit for Illumina
    NEBNext Ultra RNA Library Prep Kit for Illumina 96 rxns
    https://www.bioz.com/result/rna library prep kit/product/New England Biolabs
    Average 99 stars, based on 62 article reviews
    Price from $9.99 to $1999.99
    rna library prep kit - by Bioz Stars, 2020-07
    99/100 stars

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    Related Articles

    Multiplex Assay:

    Article Title: Integrated analysis of hepatic mRNA and miRNA profiles identified molecular networks and potential biomarkers of NAFLD
    Article Snippet: .. Libraries for mRNA and miRNA sequencing were respectively generated using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB,USA) and the NEBNext Multiplex Small RNA Library Prep Kit for Illumina (NEB,USA) according to the manufacturer’s instructions. .. Then, the final purified libraries were evaluated using a BioAnalyzer 2100 and quantified.

    Sequencing:

    Article Title: Rare cell detection by single cell RNA sequencing as guided by single molecule RNA FISH
    Article Snippet: .. We sequenced mRNA in bulk from WM989-A6 populations as per Shaffer et. al. We isolated mRNA and built sequencing libraries using the NEBNext Poly(A) mRNA Magnetic Isolation Module and NEBNext Ultra RNA Library Prep Kit for Illumina. ..

    Article Title: RNA N6-methyladenosine demethylase FTO promotes breast tumor progression through inhibiting BNIP3
    Article Snippet: .. For high-throughput sequencing, purified RNA fragments from m6A-MeRIP were used for library construction with the NEBNext Ultra RNA library Prep kit for Illumina (E7530S, NEB, USA) and were sequenced by Illumina HiSeq 2000. ..

    Article Title: Integrated analysis of hepatic mRNA and miRNA profiles identified molecular networks and potential biomarkers of NAFLD
    Article Snippet: .. Libraries for mRNA and miRNA sequencing were respectively generated using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB,USA) and the NEBNext Multiplex Small RNA Library Prep Kit for Illumina (NEB,USA) according to the manufacturer’s instructions. .. Then, the final purified libraries were evaluated using a BioAnalyzer 2100 and quantified.

    RNA HS Assay:

    Article Title: Evolution of a Designed Protein Assembly Encapsulating its Own RNA Genome
    Article Snippet: .. The purified RNA was quantitated using a Qubit RNA HS Assay Kit, and 100 ng of RNA was used to prepare each RNAseq library with a NEBNext® Ultra™ RNA Library Prep Kit for Illumina® kit (NEB, E7530S). .. Each library was PCR amplified using Kapa HiFi polymerase to add sequencing barcodes before being pooled for sequencing.

    Isolation:

    Article Title: Rare cell detection by single cell RNA sequencing as guided by single molecule RNA FISH
    Article Snippet: .. We sequenced mRNA in bulk from WM989-A6 populations as per Shaffer et. al. We isolated mRNA and built sequencing libraries using the NEBNext Poly(A) mRNA Magnetic Isolation Module and NEBNext Ultra RNA Library Prep Kit for Illumina. ..

    Article Title: Transgenerational Effects of Extended Dauer Diapause on Starvation Survival and Gene Expression Plasticity in Caenorhabditis elegans
    Article Snippet: .. RNA was isolated using TRIzol (Invitrogen) using the manufacturer’s protocol with minor modifications; 1 ml of TRIzol was used per sample along with 100 μl of acid-washed sand. mRNA-seq libraries were prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina (E7530) in two batches, utilizing either 500 or 100 ng of starting RNA per library and 12 or 15 PCR cycles, respectively. .. Libraries were sequenced using Illumina HiSeq 4000 to obtain single-end 50 bp reads.

    Purification:

    Article Title: Evolution of a Designed Protein Assembly Encapsulating its Own RNA Genome
    Article Snippet: .. The purified RNA was quantitated using a Qubit RNA HS Assay Kit, and 100 ng of RNA was used to prepare each RNAseq library with a NEBNext® Ultra™ RNA Library Prep Kit for Illumina® kit (NEB, E7530S). .. Each library was PCR amplified using Kapa HiFi polymerase to add sequencing barcodes before being pooled for sequencing.

    Article Title: RNA N6-methyladenosine demethylase FTO promotes breast tumor progression through inhibiting BNIP3
    Article Snippet: .. For high-throughput sequencing, purified RNA fragments from m6A-MeRIP were used for library construction with the NEBNext Ultra RNA library Prep kit for Illumina (E7530S, NEB, USA) and were sequenced by Illumina HiSeq 2000. ..

    High Throughput Screening Assay:

    Article Title: RNA N6-methyladenosine demethylase FTO promotes breast tumor progression through inhibiting BNIP3
    Article Snippet: .. For high-throughput sequencing, purified RNA fragments from m6A-MeRIP were used for library construction with the NEBNext Ultra RNA library Prep kit for Illumina (E7530S, NEB, USA) and were sequenced by Illumina HiSeq 2000. ..

    Generated:

    Article Title: Integrated analysis of hepatic mRNA and miRNA profiles identified molecular networks and potential biomarkers of NAFLD
    Article Snippet: .. Libraries for mRNA and miRNA sequencing were respectively generated using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB,USA) and the NEBNext Multiplex Small RNA Library Prep Kit for Illumina (NEB,USA) according to the manufacturer’s instructions. .. Then, the final purified libraries were evaluated using a BioAnalyzer 2100 and quantified.

    other:

    Article Title: RNA Sequencing by Direct Tagmentation of RNA/DNA Hybrids
    Article Snippet: Then we plotted a heatmap of the distance matrix ( ) between different cell types and library preparation methods.

    Polymerase Chain Reaction:

    Article Title: Transgenerational Effects of Extended Dauer Diapause on Starvation Survival and Gene Expression Plasticity in Caenorhabditis elegans
    Article Snippet: .. RNA was isolated using TRIzol (Invitrogen) using the manufacturer’s protocol with minor modifications; 1 ml of TRIzol was used per sample along with 100 μl of acid-washed sand. mRNA-seq libraries were prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina (E7530) in two batches, utilizing either 500 or 100 ng of starting RNA per library and 12 or 15 PCR cycles, respectively. .. Libraries were sequenced using Illumina HiSeq 4000 to obtain single-end 50 bp reads.

    End-sequence Profiling:

    Article Title: The primary transcriptome, small RNAs and regulation of antimicrobial resistance in Acinetobacter baumannii ATCC 17978
    Article Snippet: .. Libraries of the ESP RNA samples were prepared using the NEBNext™ Ultra RNA Library Prep Kit for Illumina (NEB) according to the manufacturer's instructions. .. These libraries were sequenced on an Illumina MiSeq (paired-end reads).

    In Vitro:

    Article Title: Strand-specific single-cell methylomics reveals distinct modes of DNA demethylation dynamics during early mammalian development
    Article Snippet: .. Thereafter, in vitro transcription and Illumina library preparation is performed as described previously in the scAba-seq protocol. ..

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  • 99
    New England Biolabs small rna library prep kit
    Genes with L2 in 3’UTR are bound by <t>AGO2</t> in the human brain. A) Schematics of AGO2 RIP followed by total <t>RNA</t> sequencing on cortex (CTX) biopsies. B) Genomic distribution of RNAs in the AGO2 RIP-seq and input samples of cortex (CTX, n = 2). C) Dot plot of all genes in cortex samples from the human brain. Genes with L2 in the 3’UTR are marked in red. The log 2 -transformed mean expression (x-axis) is plotted against the log 2 -transformed fold change of RIP (n = 2) versus input samples (n = 2) (y-axis). D) Cumulative fraction plot of the fold changes of all genes and genes with L2 in their 3’UTR in RIP versus INPUT samples. ***p
    Small Rna Library Prep Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 46 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/small rna library prep kit/product/New England Biolabs
    Average 99 stars, based on 46 article reviews
    Price from $9.99 to $1999.99
    small rna library prep kit - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    93
    New England Biolabs nebnext ultra rna library prep kit for illumina
    Mapping of MinION long reads, <t>Illumina-assembled</t> scaffolds, and <t>RNA-sequencing</t> reads of male and female A. ocellaris to the genomic region containing the cyp19a1a gene. Transcripts per million (TPM) values were calculated using Kallisto, version 0.43.1 [ 46 ].
    Nebnext Ultra Rna Library Prep Kit For Illumina, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nebnext ultra rna library prep kit for illumina/product/New England Biolabs
    Average 93 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    nebnext ultra rna library prep kit for illumina - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

    Image Search Results


    Genes with L2 in 3’UTR are bound by AGO2 in the human brain. A) Schematics of AGO2 RIP followed by total RNA sequencing on cortex (CTX) biopsies. B) Genomic distribution of RNAs in the AGO2 RIP-seq and input samples of cortex (CTX, n = 2). C) Dot plot of all genes in cortex samples from the human brain. Genes with L2 in the 3’UTR are marked in red. The log 2 -transformed mean expression (x-axis) is plotted against the log 2 -transformed fold change of RIP (n = 2) versus input samples (n = 2) (y-axis). D) Cumulative fraction plot of the fold changes of all genes and genes with L2 in their 3’UTR in RIP versus INPUT samples. ***p

    Journal: PLoS Genetics

    Article Title: LINE-2 transposable elements are a source of functional human microRNAs and target sites

    doi: 10.1371/journal.pgen.1008036

    Figure Lengend Snippet: Genes with L2 in 3’UTR are bound by AGO2 in the human brain. A) Schematics of AGO2 RIP followed by total RNA sequencing on cortex (CTX) biopsies. B) Genomic distribution of RNAs in the AGO2 RIP-seq and input samples of cortex (CTX, n = 2). C) Dot plot of all genes in cortex samples from the human brain. Genes with L2 in the 3’UTR are marked in red. The log 2 -transformed mean expression (x-axis) is plotted against the log 2 -transformed fold change of RIP (n = 2) versus input samples (n = 2) (y-axis). D) Cumulative fraction plot of the fold changes of all genes and genes with L2 in their 3’UTR in RIP versus INPUT samples. ***p

    Article Snippet: cDNA library preparation and sequencing of human AGO2 RIP samples cDNA library preparation was conducted using the NEB small RNA library prep kit for small RNA sequencing and the NuGen Ovation RNAseq V2 kit, followed by the Ovation Ultralow V2 Library or Ovation Rapid Library Systems, for total RNA sequencing.

    Techniques: RNA Sequencing Assay, Transformation Assay, Expressing

    AGO2-associated small RNAs in human cortex tissue. A) Schematics of AGO2 RIP-seq on human cortex samples followed by small RNA sequencing. B) Bar graph showing the percentage of reads of 20–24 nucleotides (nt) in the human genome mapping to mature miRNAs (miR), transposable elements (TE), piwi RNAs (piRNA), and transfer RNAs (tRNA). Data are represented as mean ± SEM (RIP Ctx n = 3). C) Pie chart showing the percentage of reads mapping to TEs. D) Heatmap depicting the expression of L2-miRNAs (log 2 (cpm)) across different human tissues and cell types (n = 400). AGO2—Argonaute2, AGO2 RIP—AGO2 RNA immunoprecipitation, CTX—cortex, L1—LINE-1, L2—LINE-2, LTR—long terminal repeat, mir—precursor miRNA, SINE—short interspersed nuclear element.

    Journal: PLoS Genetics

    Article Title: LINE-2 transposable elements are a source of functional human microRNAs and target sites

    doi: 10.1371/journal.pgen.1008036

    Figure Lengend Snippet: AGO2-associated small RNAs in human cortex tissue. A) Schematics of AGO2 RIP-seq on human cortex samples followed by small RNA sequencing. B) Bar graph showing the percentage of reads of 20–24 nucleotides (nt) in the human genome mapping to mature miRNAs (miR), transposable elements (TE), piwi RNAs (piRNA), and transfer RNAs (tRNA). Data are represented as mean ± SEM (RIP Ctx n = 3). C) Pie chart showing the percentage of reads mapping to TEs. D) Heatmap depicting the expression of L2-miRNAs (log 2 (cpm)) across different human tissues and cell types (n = 400). AGO2—Argonaute2, AGO2 RIP—AGO2 RNA immunoprecipitation, CTX—cortex, L1—LINE-1, L2—LINE-2, LTR—long terminal repeat, mir—precursor miRNA, SINE—short interspersed nuclear element.

    Article Snippet: cDNA library preparation and sequencing of human AGO2 RIP samples cDNA library preparation was conducted using the NEB small RNA library prep kit for small RNA sequencing and the NuGen Ovation RNAseq V2 kit, followed by the Ovation Ultralow V2 Library or Ovation Rapid Library Systems, for total RNA sequencing.

    Techniques: RNA Sequencing Assay, Expressing, Immunoprecipitation

    AGO2-associated small RNAs in the mouse brain. A) Schematics of AGO2 RIP-seq on mouse striatum (STR) followed by small RNA sequencing. B) Bar graph showing the percentage of reads of 20–24 nt in the mouse genome mapping to mature miRNAs (miR) and transposable elements (TE). Data are represented as mean ± SEM (RIP STR n = 3). C) Pie chart showing the percentage of reads mapping to TEs.

    Journal: PLoS Genetics

    Article Title: LINE-2 transposable elements are a source of functional human microRNAs and target sites

    doi: 10.1371/journal.pgen.1008036

    Figure Lengend Snippet: AGO2-associated small RNAs in the mouse brain. A) Schematics of AGO2 RIP-seq on mouse striatum (STR) followed by small RNA sequencing. B) Bar graph showing the percentage of reads of 20–24 nt in the mouse genome mapping to mature miRNAs (miR) and transposable elements (TE). Data are represented as mean ± SEM (RIP STR n = 3). C) Pie chart showing the percentage of reads mapping to TEs.

    Article Snippet: cDNA library preparation and sequencing of human AGO2 RIP samples cDNA library preparation was conducted using the NEB small RNA library prep kit for small RNA sequencing and the NuGen Ovation RNAseq V2 kit, followed by the Ovation Ultralow V2 Library or Ovation Rapid Library Systems, for total RNA sequencing.

    Techniques: RNA Sequencing Assay

    Mapping of MinION long reads, Illumina-assembled scaffolds, and RNA-sequencing reads of male and female A. ocellaris to the genomic region containing the cyp19a1a gene. Transcripts per million (TPM) values were calculated using Kallisto, version 0.43.1 [ 46 ].

    Journal: GigaScience

    Article Title: Finding Nemo: hybrid assembly with Oxford Nanopore and Illumina reads greatly improves the clownfish (Amphiprion ocellaris) genome assembly

    doi: 10.1093/gigascience/gix137

    Figure Lengend Snippet: Mapping of MinION long reads, Illumina-assembled scaffolds, and RNA-sequencing reads of male and female A. ocellaris to the genomic region containing the cyp19a1a gene. Transcripts per million (TPM) values were calculated using Kallisto, version 0.43.1 [ 46 ].

    Article Snippet: After assessing total RNA intactness on the Tapestation2100 (Agilent), mRNA was enriched using NEBNext Poly(A) mRNA Magnetic Isolation Kit (NEB, Ipwich, MA, USA) and processed with NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, Ipwich, MA, USA).

    Techniques: RNA Sequencing Assay