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Qiagen rna isolation columns
Rna Isolation Columns, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rna isolation columns/product/Qiagen
Average 99 stars, based on 1 article reviews
Price from $9.99 to $1999.99
rna isolation columns - by Bioz Stars, 2020-05
99/100 stars

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RNA Sequencing Assay:

Article Title: Effect of Bmi1 over-expression on gene expression in adult and embryonic murine neural stem cells
Article Snippet: .. High throughput RNA sequencing (RNA-seq) To prepare samples for Illumina sequencing, 150–250 ng of RNA prepared from aNSCs or eNSCs (using the RNAeasy Plus Micro kit; Qiagen) was first treated to remove ribosomal RNA using the Ribo-Zero Magnetic Kit (Epicentre, Madison, WI), using the protocol for low input samples. .. Sequencing libraries were prepared using the ScriptSeq V2 RNA-seq Kit (Epicentre), again using the low input protocol.

High Throughput Screening Assay:

Article Title: Effect of Bmi1 over-expression on gene expression in adult and embryonic murine neural stem cells
Article Snippet: .. High throughput RNA sequencing (RNA-seq) To prepare samples for Illumina sequencing, 150–250 ng of RNA prepared from aNSCs or eNSCs (using the RNAeasy Plus Micro kit; Qiagen) was first treated to remove ribosomal RNA using the Ribo-Zero Magnetic Kit (Epicentre, Madison, WI), using the protocol for low input samples. .. Sequencing libraries were prepared using the ScriptSeq V2 RNA-seq Kit (Epicentre), again using the low input protocol.

Isolation:

Article Title: Spinal neurons require Islet1 for subtype-specific differentiation of electrical excitability
Article Snippet: .. Real time quantitative PCR Total RNA was extracted from FAC sorted GFP+ cells using RNA column-based isolation kits, RNeasy® Micro kit (Qiagen). .. Concentration and integrity of the RNA extracted from FAC sorted cells were determined with an Agilent 2100 Bioanalyzer and by use of the Agilent RNA 6000 Pico Kit (Agilent Technologies, Santa Clara, CA, USA).

Article Title: Purification of high-quality RNA from a small number of fluorescence activated cell sorted zebrafish cells for RNA sequencing purposes
Article Snippet: .. The RNAqueous Micro Total RNA Isolation Kit from Ambion and the RNeasy Plus Micro Kit from Qiagen are specifically designed for purification of total RNA from a small amount of cells ( < 200,000 cells). .. In both cases, only longer RNA fragments ( > 200 nucleotides) are purified, although the workflow can be modified to isolate small RNAs such as microRNAs, 5,8S rRNA, 5S RNA, tRNA’s,… (Additional file : Figure S1).

Article Title: Cholinergic signals from the CNS regulate G-CSF-mediated HSC mobilization from bone marrow via a glucocorticoid signaling relay
Article Snippet: .. RNA for microarray analysis was isolated by sorting HSC (Lineage− Sca1+ cKit+ Flt3− directly into RLT buffer from the RNeasy Plus Micro kit (Qiagen) according to the manufacturercs instructions. .. Amplification and subsequent microarray was performed by the Genomics Core Facility at Albert Einstein College of Medicine using the One-Direct RNA amplification system (NuGEN) and GeneChip mouse gene 2.0 ST array (Affymetrix).

Article Title: Purification of high-quality RNA from a small number of fluorescence activated cell sorted zebrafish cells for RNA sequencing purposes
Article Snippet: .. Based on different quality parameters and comparison of various experimental set-ups, we recommend using the RNeasy Plus Micro Kit for RNA isolation of FACS sorted zebrafish cells. .. The RNA purification protocol is fast (only 20–30 min), easy and consistently delivers high-quality RNA samples.

Article Title: Nicotinamide Ameliorates Disease Phenotypes in a Human iPSC Model of Age-related Macular Degeneration
Article Snippet: .. To confirm that no residual RPE donor cells contaminated the hiPSC cultures, total RNA was isolated (Cat. # 74034; Qiagen) from each hiPSC line, cDNA was reverse transcribed (Cat. # 4387406; Thermo Fisher) and qPCR performed using primers specific for the RPE gene RPE65 ( ). .. hiPSC lines were grown on irradiated mouse embryonic feeders (Cat. # GSE-6301G; GlobalStem) in serum-free medium supplemented with 4ng/ml FGF2 (Cat. # 233-FB; R & D systems).

Purification:

Article Title: Purification of high-quality RNA from a small number of fluorescence activated cell sorted zebrafish cells for RNA sequencing purposes
Article Snippet: .. The RNAqueous Micro Total RNA Isolation Kit from Ambion and the RNeasy Plus Micro Kit from Qiagen are specifically designed for purification of total RNA from a small amount of cells ( < 200,000 cells). .. In both cases, only longer RNA fragments ( > 200 nucleotides) are purified, although the workflow can be modified to isolate small RNAs such as microRNAs, 5,8S rRNA, 5S RNA, tRNA’s,… (Additional file : Figure S1).

Real-time Polymerase Chain Reaction:

Article Title: Spinal neurons require Islet1 for subtype-specific differentiation of electrical excitability
Article Snippet: .. Real time quantitative PCR Total RNA was extracted from FAC sorted GFP+ cells using RNA column-based isolation kits, RNeasy® Micro kit (Qiagen). .. Concentration and integrity of the RNA extracted from FAC sorted cells were determined with an Agilent 2100 Bioanalyzer and by use of the Agilent RNA 6000 Pico Kit (Agilent Technologies, Santa Clara, CA, USA).

Article Title: Nicotinamide Ameliorates Disease Phenotypes in a Human iPSC Model of Age-related Macular Degeneration
Article Snippet: .. To confirm that no residual RPE donor cells contaminated the hiPSC cultures, total RNA was isolated (Cat. # 74034; Qiagen) from each hiPSC line, cDNA was reverse transcribed (Cat. # 4387406; Thermo Fisher) and qPCR performed using primers specific for the RPE gene RPE65 ( ). .. hiPSC lines were grown on irradiated mouse embryonic feeders (Cat. # GSE-6301G; GlobalStem) in serum-free medium supplemented with 4ng/ml FGF2 (Cat. # 233-FB; R & D systems).

Microarray:

Article Title: Cholinergic signals from the CNS regulate G-CSF-mediated HSC mobilization from bone marrow via a glucocorticoid signaling relay
Article Snippet: .. RNA for microarray analysis was isolated by sorting HSC (Lineage− Sca1+ cKit+ Flt3− directly into RLT buffer from the RNeasy Plus Micro kit (Qiagen) according to the manufacturercs instructions. .. Amplification and subsequent microarray was performed by the Genomics Core Facility at Albert Einstein College of Medicine using the One-Direct RNA amplification system (NuGEN) and GeneChip mouse gene 2.0 ST array (Affymetrix).

other:

Article Title: Nucleic acids within urinary exosomes/microvesicles are potential biomarkers for renal disease
Article Snippet: To remove genomic DNA from the Zymo processed sample, the eluted RNA was resuspended in 350 µl RLT buffer and processed using the RNeasy Plus Micro kit and eluted in 16 µl nuclease-free water.

Sequencing:

Article Title: Effect of Bmi1 over-expression on gene expression in adult and embryonic murine neural stem cells
Article Snippet: .. High throughput RNA sequencing (RNA-seq) To prepare samples for Illumina sequencing, 150–250 ng of RNA prepared from aNSCs or eNSCs (using the RNAeasy Plus Micro kit; Qiagen) was first treated to remove ribosomal RNA using the Ribo-Zero Magnetic Kit (Epicentre, Madison, WI), using the protocol for low input samples. .. Sequencing libraries were prepared using the ScriptSeq V2 RNA-seq Kit (Epicentre), again using the low input protocol.

FACS:

Article Title: Purification of high-quality RNA from a small number of fluorescence activated cell sorted zebrafish cells for RNA sequencing purposes
Article Snippet: .. Based on different quality parameters and comparison of various experimental set-ups, we recommend using the RNeasy Plus Micro Kit for RNA isolation of FACS sorted zebrafish cells. .. The RNA purification protocol is fast (only 20–30 min), easy and consistently delivers high-quality RNA samples.

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    Qiagen rna isolation columns
    <t>HCV</t> protein-protein interactions determined by co-IP. (A) Co-IP of HCV proteins using a C-specific monoclonal antibody. The wild-type and mutant JFH1 RNAs were transfected into Huh-7.5 cells as described in Materials and Methods. At 72 h p.t., the <t>RNA-transfected</t>
    Rna Isolation Columns, supplied by Qiagen, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rna isolation columns/product/Qiagen
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rna isolation columns - by Bioz Stars, 2020-05
    88/100 stars
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    HCV protein-protein interactions determined by co-IP. (A) Co-IP of HCV proteins using a C-specific monoclonal antibody. The wild-type and mutant JFH1 RNAs were transfected into Huh-7.5 cells as described in Materials and Methods. At 72 h p.t., the RNA-transfected

    Journal: Journal of Virology

    Article Title: Cell Culture-Adaptive Mutations Promote Viral Protein-Protein Interactions and Morphogenesis of Infectious Hepatitis C Virus

    doi: 10.1128/JVI.00004-12

    Figure Lengend Snippet: HCV protein-protein interactions determined by co-IP. (A) Co-IP of HCV proteins using a C-specific monoclonal antibody. The wild-type and mutant JFH1 RNAs were transfected into Huh-7.5 cells as described in Materials and Methods. At 72 h p.t., the RNA-transfected

    Article Snippet: T7 transcripts of the HCV RNA genome were purified by passing them through Qiagen RNA isolation columns.

    Techniques: Co-Immunoprecipitation Assay, Mutagenesis, Transfection