rna fragmentation reagents  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    Thermo Fisher rna fragmentation reagents
    Transcriptome-wide <t>RNA-seq</t> assays to identify potential targets of FTO inhibitors in <t>AML</t> cells.
    Rna Fragmentation Reagents, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rna fragmentation reagents/product/Thermo Fisher
    Average 99 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    rna fragmentation reagents - by Bioz Stars, 2020-01
    99/100 stars

    Images

    1) Product Images from "Small-molecule targeting of oncogenic FTO demethylase in acute myeloid leukemia"

    Article Title: Small-molecule targeting of oncogenic FTO demethylase in acute myeloid leukemia

    Journal: Cancer cell

    doi: 10.1016/j.ccell.2019.03.006

    Transcriptome-wide RNA-seq assays to identify potential targets of FTO inhibitors in AML cells.
    Figure Legend Snippet: Transcriptome-wide RNA-seq assays to identify potential targets of FTO inhibitors in AML cells.

    Techniques Used: RNA Sequencing Assay

    Related Articles

    RNA Sequencing Assay:

    Article Title: Small-molecule targeting of oncogenic FTO demethylase in acute myeloid leukemia
    Article Snippet: Paragraph title: RNA-seq and m6 A-seq assays and data analysis ... Poly(A)+ RNA was enriched from total RNA samples isolated from DMSO and FB23-2 treated MONOMAC6 AML cells for 72 hr and randomly fragmented with RNA fragmentation reagents (Ambion).

    Isolation:

    Article Title: Small-molecule targeting of oncogenic FTO demethylase in acute myeloid leukemia
    Article Snippet: .. Poly(A)+ RNA was enriched from total RNA samples isolated from DMSO and FB23-2 treated MONOMAC6 AML cells for 72 hr and randomly fragmented with RNA fragmentation reagents (Ambion). .. Poly(A)+ RNA was enriched from total RNA samples isolated from DMSO and FB23-2 treated MONOMAC6 AML cells for 72 hr and randomly fragmented with RNA fragmentation reagents (Ambion).

    Next-Generation Sequencing:

    Article Title: Small-molecule targeting of oncogenic FTO demethylase in acute myeloid leukemia
    Article Snippet: Poly(A)+ RNA was enriched from total RNA samples isolated from DMSO and FB23-2 treated MONOMAC6 AML cells for 72 hr and randomly fragmented with RNA fragmentation reagents (Ambion). .. The m6 A IP samples from both groups were subjected into next-generation sequencing on the Illumina Hiseq 2500. exomePeak ( ) with default setting was used to m6 A peak calling and Integrative Genomics Viewer (IGV) ( ) was used to visualize the distribution and abundance of m6 A peaks on each mRNA transcript.

    Construct:

    Article Title: Small-molecule targeting of oncogenic FTO demethylase in acute myeloid leukemia
    Article Snippet: Libraries were constructed with PrepX mRNA library kit (WaferGen) and sequenced to 50 bp using TruSeq SBS kit on Illumina Hiseq system. .. Poly(A)+ RNA was enriched from total RNA samples isolated from DMSO and FB23-2 treated MONOMAC6 AML cells for 72 hr and randomly fragmented with RNA fragmentation reagents (Ambion).

    Purification:

    Article Title: Small-molecule targeting of oncogenic FTO demethylase in acute myeloid leukemia
    Article Snippet: For RNA-seq assay, total RNA samples were extracted from NB4 cells upon FTO KD and inhibitors treatment for 48 hr with miRNeasy mini kit (217004, Qiagen) and poly (A)+ mRNAs were purified with NEBNext Poly(A) mRNA Magnetic Isolution Module. .. Poly(A)+ RNA was enriched from total RNA samples isolated from DMSO and FB23-2 treated MONOMAC6 AML cells for 72 hr and randomly fragmented with RNA fragmentation reagents (Ambion).

    Immunoprecipitation:

    Article Title: Small-molecule targeting of oncogenic FTO demethylase in acute myeloid leukemia
    Article Snippet: Poly(A)+ RNA was enriched from total RNA samples isolated from DMSO and FB23-2 treated MONOMAC6 AML cells for 72 hr and randomly fragmented with RNA fragmentation reagents (Ambion). .. Poly(A)+ RNA was enriched from total RNA samples isolated from DMSO and FB23-2 treated MONOMAC6 AML cells for 72 hr and randomly fragmented with RNA fragmentation reagents (Ambion).

    Expressing:

    Article Title: Small-molecule targeting of oncogenic FTO demethylase in acute myeloid leukemia
    Article Snippet: Differential gene expression was analyzed following standard Illumina sequence analysis pipeline. .. Poly(A)+ RNA was enriched from total RNA samples isolated from DMSO and FB23-2 treated MONOMAC6 AML cells for 72 hr and randomly fragmented with RNA fragmentation reagents (Ambion).

    Sequencing:

    Article Title: Small-molecule targeting of oncogenic FTO demethylase in acute myeloid leukemia
    Article Snippet: Differential gene expression was analyzed following standard Illumina sequence analysis pipeline. .. Poly(A)+ RNA was enriched from total RNA samples isolated from DMSO and FB23-2 treated MONOMAC6 AML cells for 72 hr and randomly fragmented with RNA fragmentation reagents (Ambion).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90
    Thermo Fisher rna fragmentation reagents
    Rna Fragmentation Reagents, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rna fragmentation reagents/product/Thermo Fisher
    Average 90 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    rna fragmentation reagents - by Bioz Stars, 2020-01
    90/100 stars
      Buy from Supplier

    Image Search Results