rna endogenous control  (Thermo Fisher)


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    Name:
    Total RNA Control Human
    Description:
    Human Total RNA is provided at a convenient reverse transcription ready concentration for all RT PCR reactions TaqMan Control Total RNA is from Raji cells Burkitt s lymphoma and is conveniently packaged in a PCR ready concentration for use as a control template in RT PCR reactions The Human total RNA is provided at a concentration of 50 ng µl
    Catalog Number:
    4307281
    Price:
    None
    Applications:
    PCR & Real-Time PCR|Real Time PCR (qPCR)|Real-Time PCR Primers, Probes, Arrays & Controls
    Category:
    Standards Ladders Controls
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    Structured Review

    Thermo Fisher rna endogenous control
    Human Total RNA is provided at a convenient reverse transcription ready concentration for all RT PCR reactions TaqMan Control Total RNA is from Raji cells Burkitt s lymphoma and is conveniently packaged in a PCR ready concentration for use as a control template in RT PCR reactions The Human total RNA is provided at a concentration of 50 ng µl
    https://www.bioz.com/result/rna endogenous control/product/Thermo Fisher
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    rna endogenous control - by Bioz Stars, 2020-09
    99/100 stars

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    Transfection:

    Article Title: A functional interplay between ZNF217 and Estrogen Receptor alpha exists in luminal breast cancers), A functional interplay between ZNF217 and Estrogen Receptor alpha exists in luminal breast cancers
    Article Snippet: .. Stealth™ siRNAs targeting ZNF217 (siRNA‐ZNF217‐A, leading to intermediate knock‐down of ZNF217 and siRNA‐ZNF217‐B, leading to total ZNF217 knock‐down) , ESR1 and scrambled control RNA (Life Technologies, Paisley, UK) were transfected (5 × 10−9 M) as previously described ( ). .. Briefly, 2 × 106 cells were lysed using the Subcellular Protein Fractionation Kit (Thermo Scientific, Waltham, MA, USA).

    Article Title: The novel transcriptional factor HP1BP3 negatively regulates Hsp70 transcription in Crassostrea hongkongensis
    Article Snippet: .. Quantitative real-time PCR Total RNA was extracted from hemocytes transfected with either siRNA or control RNA as well as from the gills of oysters that were either non-stressed or stressed by heat, CdCl2 , or NP at various time points using the Trizol reagent (Cat. No. 15596-026; Invitrogen). .. Two micrograms of total RNA was used as template to synthesize the first-strand cDNA with MLV Reverse transcriptase (Promega) and oligo (dT) primer.

    Article Title: PAR-1 is a novel mechano-sensor transducing laminar flow-mediated endothelial signaling
    Article Snippet: .. For PAR-1 silencing, HUVECs were transiently transfected with 100 pmol/L of control RNA or siRNA targeting human PAR-1 using Lipofectamine 2000 reagent (#11668-019, Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions. .. A non-specific control siRNA from Bioneer was used as a negative control.

    Article Title: miR-16 and miR-26a target checkpoint kinases Wee1 and Chk1 in response to p53 activation by genotoxic stress
    Article Snippet: .. For luciferase reporter assays, cells were cultured in six-well plates, and each well was transfected with 0.5 μ g of firefly luciferase reporter plasmid, 0.5 μ g of β -galactosidase expression plasmid (Ambion), and equal amounts of scrambled negative control RNA, pre-miR-26a, pre-miR-16, or anti-miR-16 using Lipofectamine 2000 (Invitrogen). .. The β -galactosidase plasmid was used as a transfection control.

    Luciferase:

    Article Title: miR-16 and miR-26a target checkpoint kinases Wee1 and Chk1 in response to p53 activation by genotoxic stress
    Article Snippet: .. For luciferase reporter assays, cells were cultured in six-well plates, and each well was transfected with 0.5 μ g of firefly luciferase reporter plasmid, 0.5 μ g of β -galactosidase expression plasmid (Ambion), and equal amounts of scrambled negative control RNA, pre-miR-26a, pre-miR-16, or anti-miR-16 using Lipofectamine 2000 (Invitrogen). .. The β -galactosidase plasmid was used as a transfection control.

    Negative Control:

    Article Title: miR-16 and miR-26a target checkpoint kinases Wee1 and Chk1 in response to p53 activation by genotoxic stress
    Article Snippet: .. For luciferase reporter assays, cells were cultured in six-well plates, and each well was transfected with 0.5 μ g of firefly luciferase reporter plasmid, 0.5 μ g of β -galactosidase expression plasmid (Ambion), and equal amounts of scrambled negative control RNA, pre-miR-26a, pre-miR-16, or anti-miR-16 using Lipofectamine 2000 (Invitrogen). .. The β -galactosidase plasmid was used as a transfection control.

    Article Title: LUC7L3/CROP inhibits replication of hepatitis B virus via suppressing enhancer II/basal core promoter activity
    Article Snippet: .. ACIN1-specific siRNA, BCLAF1-specific siRNA, CNBP1-specific siRNA, CTCF-specific siRNA, HDGFRP2-specific siRNA, HMGN1-specific siRNA, LUC7L3-specific siRNA (siLUC7) and the negative control RNA (siNC) was provided by Bonac (Fukuoka, Japan). siRNAs for HNF4α (siHNF4a), C/EBPα (siC/EBPa) and the negative control RNA (siNC-2) were purchased from Ambion (CA, USA). .. The synthetic siRNAs (50 pmol) were transfected into cells using ScreenFect A (Wako).

    Cell Culture:

    Article Title: miR-16 and miR-26a target checkpoint kinases Wee1 and Chk1 in response to p53 activation by genotoxic stress
    Article Snippet: .. For luciferase reporter assays, cells were cultured in six-well plates, and each well was transfected with 0.5 μ g of firefly luciferase reporter plasmid, 0.5 μ g of β -galactosidase expression plasmid (Ambion), and equal amounts of scrambled negative control RNA, pre-miR-26a, pre-miR-16, or anti-miR-16 using Lipofectamine 2000 (Invitrogen). .. The β -galactosidase plasmid was used as a transfection control.

    Real-time Polymerase Chain Reaction:

    Article Title: The novel transcriptional factor HP1BP3 negatively regulates Hsp70 transcription in Crassostrea hongkongensis
    Article Snippet: .. Quantitative real-time PCR Total RNA was extracted from hemocytes transfected with either siRNA or control RNA as well as from the gills of oysters that were either non-stressed or stressed by heat, CdCl2 , or NP at various time points using the Trizol reagent (Cat. No. 15596-026; Invitrogen). .. Two micrograms of total RNA was used as template to synthesize the first-strand cDNA with MLV Reverse transcriptase (Promega) and oligo (dT) primer.

    Small Interfering RNA:

    Article Title: Natriuretic peptide activation of extracellular regulated kinase 1/2 (ERK1/2) pathway by particulate guanylyl cyclases in GH3 somatolactotropes
    Article Snippet: .. For RNA interference, pre-designed GC-B short interfering RNA (siRNA) or scrambled RNA control (Ambion Biosciences, Invitrogen) were introduced into GH3 cells by the lipofectin siRNAmax reverse-transfection method (Invitrogen). .. For MAPK phosphatase-1 (MKP1) over-expression studies, 5 μg/well MKP1 or pcDNA expression vectors were reverse-transfected into GH3 cells, prior to serum starvation and subsequent stimulation.

    Expressing:

    Article Title: miR-16 and miR-26a target checkpoint kinases Wee1 and Chk1 in response to p53 activation by genotoxic stress
    Article Snippet: .. For luciferase reporter assays, cells were cultured in six-well plates, and each well was transfected with 0.5 μ g of firefly luciferase reporter plasmid, 0.5 μ g of β -galactosidase expression plasmid (Ambion), and equal amounts of scrambled negative control RNA, pre-miR-26a, pre-miR-16, or anti-miR-16 using Lipofectamine 2000 (Invitrogen). .. The β -galactosidase plasmid was used as a transfection control.

    Reporter Gene Assay:

    Article Title: MicroRNA-137 Upregulation Increases Bladder Cancer Cell Proliferation and Invasion by Targeting PAQR3
    Article Snippet: .. For the reporter gene assay, the cells were cotransfected with 0.5 µg of pGL3-PAQR3-3′UTR or pGL3- PAQR3-3′UTR Mut plasmid, 0.05 ng of the phRL-SV40 control vector (Promega, USA), and 100 nM miR-137 or control RNA using Lipofectamine 2000 (Invitrogen, USA). .. The firefly and renilla luciferase activities were measured consecutively through a dual luciferase assay (Promega, USA) 24 h after transfection.

    Plasmid Preparation:

    Article Title: MicroRNA-137 Upregulation Increases Bladder Cancer Cell Proliferation and Invasion by Targeting PAQR3
    Article Snippet: .. For the reporter gene assay, the cells were cotransfected with 0.5 µg of pGL3-PAQR3-3′UTR or pGL3- PAQR3-3′UTR Mut plasmid, 0.05 ng of the phRL-SV40 control vector (Promega, USA), and 100 nM miR-137 or control RNA using Lipofectamine 2000 (Invitrogen, USA). .. The firefly and renilla luciferase activities were measured consecutively through a dual luciferase assay (Promega, USA) 24 h after transfection.

    Article Title: miR-16 and miR-26a target checkpoint kinases Wee1 and Chk1 in response to p53 activation by genotoxic stress
    Article Snippet: .. For luciferase reporter assays, cells were cultured in six-well plates, and each well was transfected with 0.5 μ g of firefly luciferase reporter plasmid, 0.5 μ g of β -galactosidase expression plasmid (Ambion), and equal amounts of scrambled negative control RNA, pre-miR-26a, pre-miR-16, or anti-miR-16 using Lipofectamine 2000 (Invitrogen). .. The β -galactosidase plasmid was used as a transfection control.

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  • 99
    Thermo Fisher gene exp rn18s mm03928990 g1
    Q-PCR analysis of IL-2, IL-4, IL-6, IL-10, TNF-α, and IFN-γ mRNA expression in the liver of islet graft recipients treated with vehicle, or with 0.1 or 0.3 mg/kg trametinib (Tra 0.1 and Tra 0.3, respectively). Expression of each gene was normalized to that of 18s rRNA, and then quantified relative to that in naive mice. Data shown are from 3 independent experiments and are expressed as the mean ± SD (n = 3/group; * P
    Gene Exp Rn18s Mm03928990 G1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 137 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gene exp rn18s mm03928990 g1/product/Thermo Fisher
    Average 99 stars, based on 137 article reviews
    Price from $9.99 to $1999.99
    gene exp rn18s mm03928990 g1 - by Bioz Stars, 2020-09
    99/100 stars
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    99
    Thermo Fisher 18s ribosomal rna probe
    Q-PCR analysis of IL-2, IL-4, IL-6, IL-10, TNF-α, and IFN-γ mRNA expression in the liver of islet graft recipients treated with vehicle, or with 0.1 or 0.3 mg/kg trametinib (Tra 0.1 and Tra 0.3, respectively). Expression of each gene was normalized to that of 18s rRNA, and then quantified relative to that in naive mice. Data shown are from 3 independent experiments and are expressed as the mean ± SD (n = 3/group; * P
    18s Ribosomal Rna Probe, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/18s ribosomal rna probe/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    18s ribosomal rna probe - by Bioz Stars, 2020-09
    99/100 stars
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    99
    Thermo Fisher gene exp rn18s rn03928990 g1
    Q-PCR analysis of IL-2, IL-4, IL-6, IL-10, TNF-α, and IFN-γ mRNA expression in the liver of islet graft recipients treated with vehicle, or with 0.1 or 0.3 mg/kg trametinib (Tra 0.1 and Tra 0.3, respectively). Expression of each gene was normalized to that of 18s rRNA, and then quantified relative to that in naive mice. Data shown are from 3 independent experiments and are expressed as the mean ± SD (n = 3/group; * P
    Gene Exp Rn18s Rn03928990 G1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gene exp rn18s rn03928990 g1/product/Thermo Fisher
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    gene exp rn18s rn03928990 g1 - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    Image Search Results


    Q-PCR analysis of IL-2, IL-4, IL-6, IL-10, TNF-α, and IFN-γ mRNA expression in the liver of islet graft recipients treated with vehicle, or with 0.1 or 0.3 mg/kg trametinib (Tra 0.1 and Tra 0.3, respectively). Expression of each gene was normalized to that of 18s rRNA, and then quantified relative to that in naive mice. Data shown are from 3 independent experiments and are expressed as the mean ± SD (n = 3/group; * P

    Journal: Transplantation Direct

    Article Title: The MEK Inhibitor Trametinib Suppresses Major Histocompatibility Antigen-mismatched Rejection Following Pancreatic Islet Transplantation

    doi: 10.1097/TXD.0000000000001045

    Figure Lengend Snippet: Q-PCR analysis of IL-2, IL-4, IL-6, IL-10, TNF-α, and IFN-γ mRNA expression in the liver of islet graft recipients treated with vehicle, or with 0.1 or 0.3 mg/kg trametinib (Tra 0.1 and Tra 0.3, respectively). Expression of each gene was normalized to that of 18s rRNA, and then quantified relative to that in naive mice. Data shown are from 3 independent experiments and are expressed as the mean ± SD (n = 3/group; * P

    Article Snippet: 18S rRNA was used as an endogenous control (Mm03928990_g1).

    Techniques: Polymerase Chain Reaction, Expressing, Mouse Assay