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Becton Dickinson rna dna sequencing
Rna Dna Sequencing, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 92 stars, based on 2 article reviews
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rna dna sequencing - by Bioz Stars, 2020-09
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High Throughput Screening Assay:

Article Title: Platforms for Single-Cell Collection and Analysis
Article Snippet: .. For example, high-throughput instruments offer scalable kits to analyze different starting numbers of cells (ddSEQ Single-Cell Isolator, Bio-Rad (Hercules, CA, USA) and Illumina, (San Diego, CA, USA)), the possibility of performing various types of analysis with a single instrument (genome, exome, transcriptome sequencing, and profiling of immune cells in the Chromium system, 10x Genomics (Pleasanton, CA, USA)), specific or customized panels for targeted RNA/DNA sequencing (Rhapsody Single-Cell Analysis System, Becton, Dickinson and Company (BD, Franklin Lakes, NJ, USA) and Tapestri Platform (MissionBio, San Francisco, CA, USA)), and completely controllable parameters of single-cell collection (Nadia Innovate, Dolomite Bio, Royston, UK). .. On the other hand, providers of low-throughput solutions try to increase the throughput (new C1 chip for up to 800 cells from Fluidigm (South San Francisco, CA, USA)), offer end-to-end workflows from cell capture to reporting (WTA and WGA kits by Menarini Silicon Biosystems for the DEPArray System (Florence, Italy)), give the possibility of cultivating single cells (CellRaft AIR System, CellMicrosystems (Research Triangle Park, NC, USA)), and even stimulate single cells and analyze their responses - Polaris platform from Fluidigm (South San Francisco, CA, USA).

Sequencing:

Article Title: Platforms for Single-Cell Collection and Analysis
Article Snippet: .. For example, high-throughput instruments offer scalable kits to analyze different starting numbers of cells (ddSEQ Single-Cell Isolator, Bio-Rad (Hercules, CA, USA) and Illumina, (San Diego, CA, USA)), the possibility of performing various types of analysis with a single instrument (genome, exome, transcriptome sequencing, and profiling of immune cells in the Chromium system, 10x Genomics (Pleasanton, CA, USA)), specific or customized panels for targeted RNA/DNA sequencing (Rhapsody Single-Cell Analysis System, Becton, Dickinson and Company (BD, Franklin Lakes, NJ, USA) and Tapestri Platform (MissionBio, San Francisco, CA, USA)), and completely controllable parameters of single-cell collection (Nadia Innovate, Dolomite Bio, Royston, UK). .. On the other hand, providers of low-throughput solutions try to increase the throughput (new C1 chip for up to 800 cells from Fluidigm (South San Francisco, CA, USA)), offer end-to-end workflows from cell capture to reporting (WTA and WGA kits by Menarini Silicon Biosystems for the DEPArray System (Florence, Italy)), give the possibility of cultivating single cells (CellRaft AIR System, CellMicrosystems (Research Triangle Park, NC, USA)), and even stimulate single cells and analyze their responses - Polaris platform from Fluidigm (South San Francisco, CA, USA).

Single-cell Analysis:

Article Title: Platforms for Single-Cell Collection and Analysis
Article Snippet: .. For example, high-throughput instruments offer scalable kits to analyze different starting numbers of cells (ddSEQ Single-Cell Isolator, Bio-Rad (Hercules, CA, USA) and Illumina, (San Diego, CA, USA)), the possibility of performing various types of analysis with a single instrument (genome, exome, transcriptome sequencing, and profiling of immune cells in the Chromium system, 10x Genomics (Pleasanton, CA, USA)), specific or customized panels for targeted RNA/DNA sequencing (Rhapsody Single-Cell Analysis System, Becton, Dickinson and Company (BD, Franklin Lakes, NJ, USA) and Tapestri Platform (MissionBio, San Francisco, CA, USA)), and completely controllable parameters of single-cell collection (Nadia Innovate, Dolomite Bio, Royston, UK). .. On the other hand, providers of low-throughput solutions try to increase the throughput (new C1 chip for up to 800 cells from Fluidigm (South San Francisco, CA, USA)), offer end-to-end workflows from cell capture to reporting (WTA and WGA kits by Menarini Silicon Biosystems for the DEPArray System (Florence, Italy)), give the possibility of cultivating single cells (CellRaft AIR System, CellMicrosystems (Research Triangle Park, NC, USA)), and even stimulate single cells and analyze their responses - Polaris platform from Fluidigm (South San Francisco, CA, USA).

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    Becton Dickinson rna
    Quantitative analysis of the viral gene of rotavirus adsorbed onto the antibody-integrated MNBs. Notes: Rotavirus-infected cell lysate (10 µL) was diluted with PBS (500 µL) and then incubated with antibody-integrated magnetic beads. After incubation, the following fractions were obtained: 1) diluted rotavirus sample before incubation with the beads (BF), 2) bead fraction after incubation with anti-rotavirus antibody-integrated MNBs (RV-BD), 3) bead fraction after incubation with anti-dengue virus antibody-integrated MNBs (DV-BD), 4) supernatant fraction after incubation with the anti-rotavirus antibody-integrated MNBs (RV-SP), 5) supernatant fraction after incubation with the anti-dengue virus antibody-integrated MNBs (DV-SP), and 6) total sample containing the same quantity of rotavirus as in 10 µL of rotavirus-infected cell lysate (total fraction, TL). Viral genomic <t>RNA</t> was subsequently extracted from the above fractions using a QIAamp Viral RNA mini kit and subjected to RT-reaction. The resultant cDNA was analyzed by real-time PCR using primers for the rotavirus <t>VP7</t> gene as described in Materials and methods. The value of the TL sample was taken as 100%. Abbreviations: MNBs, magnetic nanobeads; RT, reverse transcription.
    Rna, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 92/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rna/product/Becton Dickinson
    Average 92 stars, based on 15 article reviews
    Price from $9.99 to $1999.99
    rna - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    93
    Becton Dickinson high throughput rna sequencing
    Quantitative analysis of the viral gene of rotavirus adsorbed onto the antibody-integrated MNBs. Notes: Rotavirus-infected cell lysate (10 µL) was diluted with PBS (500 µL) and then incubated with antibody-integrated magnetic beads. After incubation, the following fractions were obtained: 1) diluted rotavirus sample before incubation with the beads (BF), 2) bead fraction after incubation with anti-rotavirus antibody-integrated MNBs (RV-BD), 3) bead fraction after incubation with anti-dengue virus antibody-integrated MNBs (DV-BD), 4) supernatant fraction after incubation with the anti-rotavirus antibody-integrated MNBs (RV-SP), 5) supernatant fraction after incubation with the anti-dengue virus antibody-integrated MNBs (DV-SP), and 6) total sample containing the same quantity of rotavirus as in 10 µL of rotavirus-infected cell lysate (total fraction, TL). Viral genomic <t>RNA</t> was subsequently extracted from the above fractions using a QIAamp Viral RNA mini kit and subjected to RT-reaction. The resultant cDNA was analyzed by real-time PCR using primers for the rotavirus <t>VP7</t> gene as described in Materials and methods. The value of the TL sample was taken as 100%. Abbreviations: MNBs, magnetic nanobeads; RT, reverse transcription.
    High Throughput Rna Sequencing, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/high throughput rna sequencing/product/Becton Dickinson
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    high throughput rna sequencing - by Bioz Stars, 2020-09
    93/100 stars
      Buy from Supplier

    92
    Becton Dickinson rna dna sequencing
    Quantitative analysis of the viral gene of rotavirus adsorbed onto the antibody-integrated MNBs. Notes: Rotavirus-infected cell lysate (10 µL) was diluted with PBS (500 µL) and then incubated with antibody-integrated magnetic beads. After incubation, the following fractions were obtained: 1) diluted rotavirus sample before incubation with the beads (BF), 2) bead fraction after incubation with anti-rotavirus antibody-integrated MNBs (RV-BD), 3) bead fraction after incubation with anti-dengue virus antibody-integrated MNBs (DV-BD), 4) supernatant fraction after incubation with the anti-rotavirus antibody-integrated MNBs (RV-SP), 5) supernatant fraction after incubation with the anti-dengue virus antibody-integrated MNBs (DV-SP), and 6) total sample containing the same quantity of rotavirus as in 10 µL of rotavirus-infected cell lysate (total fraction, TL). Viral genomic <t>RNA</t> was subsequently extracted from the above fractions using a QIAamp Viral RNA mini kit and subjected to RT-reaction. The resultant cDNA was analyzed by real-time PCR using primers for the rotavirus <t>VP7</t> gene as described in Materials and methods. The value of the TL sample was taken as 100%. Abbreviations: MNBs, magnetic nanobeads; RT, reverse transcription.
    Rna Dna Sequencing, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rna dna sequencing/product/Becton Dickinson
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    rna dna sequencing - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

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    Quantitative analysis of the viral gene of rotavirus adsorbed onto the antibody-integrated MNBs. Notes: Rotavirus-infected cell lysate (10 µL) was diluted with PBS (500 µL) and then incubated with antibody-integrated magnetic beads. After incubation, the following fractions were obtained: 1) diluted rotavirus sample before incubation with the beads (BF), 2) bead fraction after incubation with anti-rotavirus antibody-integrated MNBs (RV-BD), 3) bead fraction after incubation with anti-dengue virus antibody-integrated MNBs (DV-BD), 4) supernatant fraction after incubation with the anti-rotavirus antibody-integrated MNBs (RV-SP), 5) supernatant fraction after incubation with the anti-dengue virus antibody-integrated MNBs (DV-SP), and 6) total sample containing the same quantity of rotavirus as in 10 µL of rotavirus-infected cell lysate (total fraction, TL). Viral genomic RNA was subsequently extracted from the above fractions using a QIAamp Viral RNA mini kit and subjected to RT-reaction. The resultant cDNA was analyzed by real-time PCR using primers for the rotavirus VP7 gene as described in Materials and methods. The value of the TL sample was taken as 100%. Abbreviations: MNBs, magnetic nanobeads; RT, reverse transcription.

    Journal: International Journal of Nanomedicine

    Article Title: Efficient recovery and enrichment of infectious rotavirus using separation with antibody-integrated graphite-encapsulated magnetic nanobeads produced by argon/ammonia gas plasma technology

    doi: 10.2147/IJN.S191784

    Figure Lengend Snippet: Quantitative analysis of the viral gene of rotavirus adsorbed onto the antibody-integrated MNBs. Notes: Rotavirus-infected cell lysate (10 µL) was diluted with PBS (500 µL) and then incubated with antibody-integrated magnetic beads. After incubation, the following fractions were obtained: 1) diluted rotavirus sample before incubation with the beads (BF), 2) bead fraction after incubation with anti-rotavirus antibody-integrated MNBs (RV-BD), 3) bead fraction after incubation with anti-dengue virus antibody-integrated MNBs (DV-BD), 4) supernatant fraction after incubation with the anti-rotavirus antibody-integrated MNBs (RV-SP), 5) supernatant fraction after incubation with the anti-dengue virus antibody-integrated MNBs (DV-SP), and 6) total sample containing the same quantity of rotavirus as in 10 µL of rotavirus-infected cell lysate (total fraction, TL). Viral genomic RNA was subsequently extracted from the above fractions using a QIAamp Viral RNA mini kit and subjected to RT-reaction. The resultant cDNA was analyzed by real-time PCR using primers for the rotavirus VP7 gene as described in Materials and methods. The value of the TL sample was taken as 100%. Abbreviations: MNBs, magnetic nanobeads; RT, reverse transcription.

    Article Snippet: This analysis showed that the amount of viral RNA corresponding to coat protein VP7 in the anti-rotavirus antibody-integrated bead fraction (RV-BD) was 82.9%±6.9% compared to the TL fraction (104.3%±8.6%).

    Techniques: Infection, Incubation, Magnetic Beads, Real-time Polymerase Chain Reaction

    Detection of viral RNA of rotavirus adsorbed onto antibody-integrated MNBs. Notes: Rotavirus-infected cell lysate (10 µL) was diluted with PBS (500 µL) and then incubated with antibody-integrated magnetic beads. After incubation, the following fractions were obtained: 1) diluted rotavirus sample before incubation with the beads (BF), 2) bead fraction after incubation with anti-rotavirus antibody-integrated MNBs (RV-BD), 3) bead fraction after incubation with anti-dengue virus antibody-integrated MNBs (DV-BD), 4) supernatant fraction after incubation with the anti-rotavirus antibody-integrated MNBs (RV-SP), 5) supernatant fraction after incubation with the anti-dengue virus antibody-integrated MNBs (DV-SP), and 6) total sample containing the same quantity of rotavirus as in 10 µL of rotavirus-infected cell lysate (total fraction, TL). Viral genomic RNA was subsequently extracted from the above fractions using a QIAamp Viral RNA mini kit and subjected to a RT-reaction. Rotavirus viral protein 7 (VP7) gene (552 bp) in the cDNA was amplified by PCR as described in Materials and methods. PCR products were analyzed by agarose gel electrophoresis (1.2% gel). The identity of the amplified products was confirmed by DNA sequencing. The left-hand lane is size marker (M), which includes DNA of 100, 200, 300, 400, 500, 600, 700, 800, 900, 1,000, 1,200, and 1,500 bp. The position of the 552 bp band for VP7 is indicated by an arrow. The NC comprised a water sample (no rotavirus) that was subjected to RT-PCR. Abbreviations: MNBs, magnetic nanobeads; NC, negative control; RT, reverse transcription.

    Journal: International Journal of Nanomedicine

    Article Title: Efficient recovery and enrichment of infectious rotavirus using separation with antibody-integrated graphite-encapsulated magnetic nanobeads produced by argon/ammonia gas plasma technology

    doi: 10.2147/IJN.S191784

    Figure Lengend Snippet: Detection of viral RNA of rotavirus adsorbed onto antibody-integrated MNBs. Notes: Rotavirus-infected cell lysate (10 µL) was diluted with PBS (500 µL) and then incubated with antibody-integrated magnetic beads. After incubation, the following fractions were obtained: 1) diluted rotavirus sample before incubation with the beads (BF), 2) bead fraction after incubation with anti-rotavirus antibody-integrated MNBs (RV-BD), 3) bead fraction after incubation with anti-dengue virus antibody-integrated MNBs (DV-BD), 4) supernatant fraction after incubation with the anti-rotavirus antibody-integrated MNBs (RV-SP), 5) supernatant fraction after incubation with the anti-dengue virus antibody-integrated MNBs (DV-SP), and 6) total sample containing the same quantity of rotavirus as in 10 µL of rotavirus-infected cell lysate (total fraction, TL). Viral genomic RNA was subsequently extracted from the above fractions using a QIAamp Viral RNA mini kit and subjected to a RT-reaction. Rotavirus viral protein 7 (VP7) gene (552 bp) in the cDNA was amplified by PCR as described in Materials and methods. PCR products were analyzed by agarose gel electrophoresis (1.2% gel). The identity of the amplified products was confirmed by DNA sequencing. The left-hand lane is size marker (M), which includes DNA of 100, 200, 300, 400, 500, 600, 700, 800, 900, 1,000, 1,200, and 1,500 bp. The position of the 552 bp band for VP7 is indicated by an arrow. The NC comprised a water sample (no rotavirus) that was subjected to RT-PCR. Abbreviations: MNBs, magnetic nanobeads; NC, negative control; RT, reverse transcription.

    Article Snippet: This analysis showed that the amount of viral RNA corresponding to coat protein VP7 in the anti-rotavirus antibody-integrated bead fraction (RV-BD) was 82.9%±6.9% compared to the TL fraction (104.3%±8.6%).

    Techniques: Infection, Incubation, Magnetic Beads, Amplification, Polymerase Chain Reaction, Agarose Gel Electrophoresis, DNA Sequencing, Marker, Reverse Transcription Polymerase Chain Reaction, Negative Control