rna clean  (Zymo Research)


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    Structured Review

    Zymo Research rna clean
    Rna Clean, supplied by Zymo Research, used in various techniques. Bioz Stars score: 99/100, based on 71 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rna clean/product/Zymo Research
    Average 99 stars, based on 71 article reviews
    Price from $9.99 to $1999.99
    rna clean - by Bioz Stars, 2020-01
    99/100 stars

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    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: Aging impairs double‐strand break repair by homologous recombination in Drosophila germ cells
    Article Snippet: Paragraph title: qPCR analysis of genes in HR pathway ... Two flies were combined to represent one biological replicate, and five biological replicates per age were harvested. mRNA was purified by acid guanidinium thiocyanate–phenol–chloroform extraction with TRIzol® LS (Invitrogen; Carlsbad, CA, USA) and RNA Clean and Concentration‐5 (Zymo Research; Irvine, CA, USA).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Lack of de novo Phosphatidylinositol Synthesis Leads to Endoplasmic Reticulum Stress and Hepatic Steatosis in cdipt-Deficient Zebrafish
    Article Snippet: Paragraph title: RT-PCR, morpholino knockdown and mRNA rescue ... Cdipt mRNA was synthesized from a full-length linear DNA template using mMessage (Ambion) and purified by RNA clean™ (Zymo Research).

    RNA Sequencing Assay:

    Article Title: Global Regulation of Plant Immunity by Histone Lysine Methyl Transferases
    Article Snippet: Paragraph title: RNA-Seq and Data Analysis ... Total RNA was extracted, treated with DNase, and purified with the RNA Clean and Concentration-25 (Zymo Research).

    Synthesized:

    Article Title: Lack of de novo Phosphatidylinositol Synthesis Leads to Endoplasmic Reticulum Stress and Hepatic Steatosis in cdipt-Deficient Zebrafish
    Article Snippet: .. Cdipt mRNA was synthesized from a full-length linear DNA template using mMessage (Ambion) and purified by RNA clean™ (Zymo Research). .. Cdipt and gfp mRNAs were injected into 1-cell stage embryos.

    Isolation:

    Article Title: Global Regulation of Plant Immunity by Histone Lysine Methyl Transferases
    Article Snippet: Total RNA was extracted, treated with DNase, and purified with the RNA Clean and Concentration-25 (Zymo Research). .. Total RNA was isolated as described in the protocol of Spectrum Plant Total RNA Kit with on-column DNase digestion (Sigma-Aldrich).

    Spectrophotometry:

    Article Title: Global Regulation of Plant Immunity by Histone Lysine Methyl Transferases
    Article Snippet: Total RNA was extracted, treated with DNase, and purified with the RNA Clean and Concentration-25 (Zymo Research). .. The quality of the total RNA was determined by a NanoDrop spectrophotometer and an Agilent 2100 Bioanalyzer.

    Purification:

    Article Title: Global Regulation of Plant Immunity by Histone Lysine Methyl Transferases
    Article Snippet: .. Total RNA was extracted, treated with DNase, and purified with the RNA Clean and Concentration-25 (Zymo Research). .. The quality of the total RNA was determined by a NanoDrop spectrophotometer and an Agilent 2100 Bioanalyzer.

    Article Title: Aging impairs double‐strand break repair by homologous recombination in Drosophila germ cells
    Article Snippet: .. Two flies were combined to represent one biological replicate, and five biological replicates per age were harvested. mRNA was purified by acid guanidinium thiocyanate–phenol–chloroform extraction with TRIzol® LS (Invitrogen; Carlsbad, CA, USA) and RNA Clean and Concentration‐5 (Zymo Research; Irvine, CA, USA). .. Contaminating DNA was removed by DNA‐free ™ rDNase I treatment (Invitrogen).

    Article Title: Lack of de novo Phosphatidylinositol Synthesis Leads to Endoplasmic Reticulum Stress and Hepatic Steatosis in cdipt-Deficient Zebrafish
    Article Snippet: .. Cdipt mRNA was synthesized from a full-length linear DNA template using mMessage (Ambion) and purified by RNA clean™ (Zymo Research). .. Cdipt and gfp mRNAs were injected into 1-cell stage embryos.

    SYBR Green Assay:

    Article Title: Aging impairs double‐strand break repair by homologous recombination in Drosophila germ cells
    Article Snippet: Two flies were combined to represent one biological replicate, and five biological replicates per age were harvested. mRNA was purified by acid guanidinium thiocyanate–phenol–chloroform extraction with TRIzol® LS (Invitrogen; Carlsbad, CA, USA) and RNA Clean and Concentration‐5 (Zymo Research; Irvine, CA, USA). .. Reverse transcription was performed using SuperScript™ VILO Master Mix (Invitrogen), and quantitative real‐time polymerase chain reaction (qPCR) was primed with the RT2 SYBR Green Master Mix, using RT2 qPCR Primers for Drosophila melanogaster rad51, rpl32, rad50, CG5872 (ctip), gapdh2, and rad54 (cat. n.:(PPD10573A, PPD10569B, PPD05479A, PPD07756A, PPD01317A, and PPD02120A, respectively, from Qiagen; Hilden, Germany). gapdh2 and rpl32 were used for normalization due to low expression variation with age (Ling & Salvaterra, ).

    Concentration Assay:

    Article Title: Global Regulation of Plant Immunity by Histone Lysine Methyl Transferases
    Article Snippet: .. Total RNA was extracted, treated with DNase, and purified with the RNA Clean and Concentration-25 (Zymo Research). .. The quality of the total RNA was determined by a NanoDrop spectrophotometer and an Agilent 2100 Bioanalyzer.

    Article Title: Aging impairs double‐strand break repair by homologous recombination in Drosophila germ cells
    Article Snippet: .. Two flies were combined to represent one biological replicate, and five biological replicates per age were harvested. mRNA was purified by acid guanidinium thiocyanate–phenol–chloroform extraction with TRIzol® LS (Invitrogen; Carlsbad, CA, USA) and RNA Clean and Concentration‐5 (Zymo Research; Irvine, CA, USA). .. Contaminating DNA was removed by DNA‐free ™ rDNase I treatment (Invitrogen).

    Expressing:

    Article Title: Aging impairs double‐strand break repair by homologous recombination in Drosophila germ cells
    Article Snippet: Two flies were combined to represent one biological replicate, and five biological replicates per age were harvested. mRNA was purified by acid guanidinium thiocyanate–phenol–chloroform extraction with TRIzol® LS (Invitrogen; Carlsbad, CA, USA) and RNA Clean and Concentration‐5 (Zymo Research; Irvine, CA, USA). .. Reverse transcription was performed using SuperScript™ VILO Master Mix (Invitrogen), and quantitative real‐time polymerase chain reaction (qPCR) was primed with the RT2 SYBR Green Master Mix, using RT2 qPCR Primers for Drosophila melanogaster rad51, rpl32, rad50, CG5872 (ctip), gapdh2, and rad54 (cat. n.:(PPD10573A, PPD10569B, PPD05479A, PPD07756A, PPD01317A, and PPD02120A, respectively, from Qiagen; Hilden, Germany). gapdh2 and rpl32 were used for normalization due to low expression variation with age (Ling & Salvaterra, ).

    Polymerase Chain Reaction:

    Article Title: Lack of de novo Phosphatidylinositol Synthesis Leads to Endoplasmic Reticulum Stress and Hepatic Steatosis in cdipt-Deficient Zebrafish
    Article Snippet: Oligo dT primed cDNA was then synthesized using SuperScript II RT (Invitrogen) and probed by PCR. .. Cdipt mRNA was synthesized from a full-length linear DNA template using mMessage (Ambion) and purified by RNA clean™ (Zymo Research).

    Sample Prep:

    Article Title: Global Regulation of Plant Immunity by Histone Lysine Methyl Transferases
    Article Snippet: Total RNA was extracted, treated with DNase, and purified with the RNA Clean and Concentration-25 (Zymo Research). .. For each sample, 3-µg of total RNA was used to prepare the mRNA-seq library according to the TrueSeq RNA Sample Prep Kit protocol (Illumina).

    Injection:

    Article Title: Lack of de novo Phosphatidylinositol Synthesis Leads to Endoplasmic Reticulum Stress and Hepatic Steatosis in cdipt-Deficient Zebrafish
    Article Snippet: Cdipt mRNA was synthesized from a full-length linear DNA template using mMessage (Ambion) and purified by RNA clean™ (Zymo Research). .. Cdipt and gfp mRNAs were injected into 1-cell stage embryos.

    Software:

    Article Title: Aging impairs double‐strand break repair by homologous recombination in Drosophila germ cells
    Article Snippet: Two flies were combined to represent one biological replicate, and five biological replicates per age were harvested. mRNA was purified by acid guanidinium thiocyanate–phenol–chloroform extraction with TRIzol® LS (Invitrogen; Carlsbad, CA, USA) and RNA Clean and Concentration‐5 (Zymo Research; Irvine, CA, USA). .. All qPCR measurements were obtained using the 7900HT Real‐Time Thermal Cycler and software (Applied BioSystems; Carlsbad, CA).

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  • 90
    Zymo Research rna purification kit
    Rna Purification Kit, supplied by Zymo Research, used in various techniques. Bioz Stars score: 90/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Zymo Research r1016
    R1016, supplied by Zymo Research, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/r1016/product/Zymo Research
    Average 90 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
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    90/100 stars
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