rna 6000 pico labchip kit  (Agilent technologies)

Journal: Diagnostic Pathology

Article Title: Enhanced molecular analyses by combination of the HOPE-technique and laser microdissection

doi: 10.1186/1746-1596-1-2

Figure Lengend Snippet: Results of RNA-integrity-testing by a Agilent Bioanalyzer 2100 using the RNA 6000 Pico LabChip kit. Lane 1 and 2 are HOPE-fixed materials and lane 3 is FFPE.

Article Snippet: Of each RNA sample 1 μl was tested in an Agilent Bioanalyzer 2100 using the RNA 6000 Pico LabChip kit (Agilent, Waldbronn, Germany, #5065-4473) to determine the RNA integrity.

Techniques: Formalin-fixed Paraffin-Embedded

Journal: Scientific Data

Article Title: Microarray data of transcriptome shifts in blood cell subsets during S1P receptor modulator therapy

doi: 10.1038/sdata.2018.145

Figure Lengend Snippet: Integrity of the RNA samples. Each bar depicts the arithmetic mean of the RNA integrity numbers (RIN) measured for the 10 whole RNA samples, which were used for the microarray hybridisation per cell population and per time point. The error bars indicate standard deviations. This quality control was carried out using the Agilent Bioanalyzer 2100 and the RNA 6000 Pico LabChip kit (Agilent Technologies, Waldbronn, Germany).

Article Snippet: The integrity of the RNA samples was checked using an Agilent Bioanalyzer 2100 with the RNA 6000 Pico LabChip kit (both from Agilent Technologies, Waldbronn, Germany).

Techniques: Microarray, Hybridization

Journal: Scientific Data

Article Title: Microarray data of transcriptome shifts in blood cell subsets during S1P receptor modulator therapy

doi: 10.1038/sdata.2018.145

Figure Lengend Snippet: Integrity of the RNA samples. Each bar depicts the arithmetic mean of the RNA integrity numbers (RIN) measured for the 10 whole RNA samples, which were used for the microarray hybridisation per cell population and per time point. The error bars indicate standard deviations. This quality control was carried out using the Agilent Bioanalyzer 2100 and the RNA 6000 Pico LabChip kit (Agilent Technologies, Waldbronn, Germany).

Article Snippet: The integrity of the RNA samples was checked using an Agilent Bioanalyzer 2100 with the RNA 6000 Pico LabChip kit (both from Agilent Technologies, Waldbronn, Germany).

Techniques: Microarray, Hybridization

Journal: bioRxiv

Article Title: A metallo-beta-lactamase with both beta-lactamase and ribonuclease activity is linked with traduction in giant viruses

doi: 10.1101/819797

Figure Lengend Snippet: Digital gel images of RNase activity of expressed Tupanvirus protein TupBlac on E. coli RNA. RNA samples (1 μg) were incubated with 15 μg of TupBlac at 30°C in the absence or presence of 10 μg/mL of sulbactam or 200 μM of ceftriaxone. Nuclease activity was visualized as digital gel images performed using the Agilent Bioanalyzer 2100 with the RNA 6000 Pico LabChip (Agilent Technologies, Palo Alto, CA). a: RNA used as substrate was from Escherichia coli; no treatment (a); buffer (b); sulbactam (c); TupBlac in the absence (d) or presence (e) of sulbactam. b: RNA used as substrate was from Escherichia coli; reactions were stopped at different times (5 min, 10 min, 30 min, 1 h and 2 h) by the addition of proteinase K (10 μg) and incubation for 1h at 37°C. The first lane corresponds to no treatment; lanes 2 to 6 to RNA treatment with TupBlac in the absence of ceftriaxone; lanes 7 to 11 to RNA treatment with TupBlac in the presence of ceftriaxone. c: nuclease activity on RNAs originating from Acanthamoeba castellanii ; no treatment (a); buffer (b); TupBlac in the absence (c, d) or presence (e) of sulbactam. d: nuclease activity on RNAs originating from bacteria that differ by the G+C-content of their genome, as indicated at the top of the digital gel image. For each RNA, three samples were analyzed: no treatment (1); treatment with TupBlac in the absence of sulbactam (2); and treatment with TupBlac in the presence of sulbactam (3).

Article Snippet: Enzymatic reactions were performed by incubating each polynucleotide (2 μg) with 15 μg of the expressed Tupanvirus protein TupBlac in Tris-HCl buffer 50 mM, pH 8.0, sodium chloride 0.3 M, using a final volume of 20 μL at 30°C for 2 h. After incubation, the material was loaded onto denaturing polyacrylamide gel electrophoresis (dPAGE) at 12% or analysed using the Agilent RNA 6000 Pico LabChip kit on an Agilent 2100 Bioanalyzer (Agilent Technology, Palo Alto, CA, USA).

Techniques: Activity Assay, Incubation