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Agilent technologies rna 6000 pico labchip kit
Results of RNA-integrity-testing by a Agilent Bioanalyzer 2100 using the RNA 6000 Pico <t>LabChip</t> kit. Lane 1 and 2 are HOPE-fixed materials and lane 3 is FFPE.
Rna 6000 Pico Labchip Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 97/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Images

1) Product Images from "Enhanced molecular analyses by combination of the HOPE-technique and laser microdissection"

Article Title: Enhanced molecular analyses by combination of the HOPE-technique and laser microdissection

Journal: Diagnostic Pathology

doi: 10.1186/1746-1596-1-2

Results of RNA-integrity-testing by a Agilent Bioanalyzer 2100 using the RNA 6000 Pico LabChip kit. Lane 1 and 2 are HOPE-fixed materials and lane 3 is FFPE.
Figure Legend Snippet: Results of RNA-integrity-testing by a Agilent Bioanalyzer 2100 using the RNA 6000 Pico LabChip kit. Lane 1 and 2 are HOPE-fixed materials and lane 3 is FFPE.

Techniques Used: Formalin-fixed Paraffin-Embedded

2) Product Images from "Microarray data of transcriptome shifts in blood cell subsets during S1P receptor modulator therapy"

Article Title: Microarray data of transcriptome shifts in blood cell subsets during S1P receptor modulator therapy

Journal: Scientific Data

doi: 10.1038/sdata.2018.145

Integrity of the RNA samples. Each bar depicts the arithmetic mean of the RNA integrity numbers (RIN) measured for the 10 whole RNA samples, which were used for the microarray hybridisation per cell population and per time point. The error bars indicate standard deviations. This quality control was carried out using the Agilent Bioanalyzer 2100 and the RNA 6000 Pico LabChip kit (Agilent Technologies, Waldbronn, Germany).
Figure Legend Snippet: Integrity of the RNA samples. Each bar depicts the arithmetic mean of the RNA integrity numbers (RIN) measured for the 10 whole RNA samples, which were used for the microarray hybridisation per cell population and per time point. The error bars indicate standard deviations. This quality control was carried out using the Agilent Bioanalyzer 2100 and the RNA 6000 Pico LabChip kit (Agilent Technologies, Waldbronn, Germany).

Techniques Used: Microarray, Hybridization

Related Articles

Cell Isolation:

Article Title: A gene expression signature that correlates with CD8+T cell expansion in acute Epstein Barr virus infection
Article Snippet: CD8+T Cells were isolated from thawed PBMC using a MACSR CD8+ T Cell Isolation Kit (Miltenyi Biotech, Cambridge MA). .. All samples were analyzed for RNA integrity on the RNA 6000 Pico Labchip kit (Agilent Technologies, Palo Alto, CA).

Amplification:

Article Title: Comparative Time-Scale Gene Expression Analysis Highlights the Infection Processes of Two Amoebophrya Strains
Article Snippet: RNA quality was assessed by capillary electrophoresis on an Agilent Bioanalyzer, using the RNA 6000 Pico LabChip kit (Agilent Technologies, Santa Clara, CA). .. Then, the second strand was generated to create double-stranded cDNA.

Article Title: Genomic and Metabolic Studies of the Impact of Probiotics on a Model Gut Symbiont and HostHow Bacterial Communities Expand Functional RepertoiresIn the Gut's Microbial Community, One Plus One Equals Many (Effects)
Article Snippet: RNA quality was assessed on a 2100 Bioanalyzer using an RNA 6000 Pico LabChip kit (Agilent Technologies). .. Intact RNA was then pooled from each group (n = 4 mice/group), with each animal contributing an equivalent mass (1.25 ng) of material.

Centrifugation:

Article Title: Comparative Time-Scale Gene Expression Analysis Highlights the Infection Processes of Two Amoebophrya Strains
Article Snippet: Then, after addition of an equal volume of EtOH 100% and vortexing, the mixture was loaded into a Zymo-SpinIIC column and centrifuged for 1 min at 12,000 g. The loading and centrifugation steps were repeated until exhaustion of the mixture. .. RNA quality was assessed by capillary electrophoresis on an Agilent Bioanalyzer, using the RNA 6000 Pico LabChip kit (Agilent Technologies, Santa Clara, CA).

Electrophoresis:

Article Title: Deciphering the complex role of thrombospondin-1 in glioblastoma development
Article Snippet: Total RNA was extracted from microdissected tissues using the RNeasy® mini Kit (Qiagen, Hilden, Germany) according to the manufacturer’s protocol. .. The integrity of the RNA was checked by capillary electrophoresis using the RNA 6000 Pico Labchip kit and the Bioanalyser 2100 (Agilent Technologies, Massy, France), and quantity was estimated using a Nanodrop 1000 (Thermo Scientific, Waltham, USA). .. The RNA integrity number (RIN) above 7/8.

Article Title: Genome-Wide Screening of Genes Regulated by DNA Methylation in Colon Cancer Development
Article Snippet: Total RNA was extracted from the microdissected tissue samples and from the treated HT-29 cells using RNeasy Micro Kit (Qiagen) according to the manufacturer’s instruction. .. The quality of the isolated nucleic acid was measured by microcapillary electrophoresis system using RNA 6000 Pico LabChip kit (Agilent BioAnalyzer 2100). .. For the microarray analysis 5–50 ng total RNA was used from the samples with RIN (RNA Integrity Number) between 7–10.

Article Title: Comparative Time-Scale Gene Expression Analysis Highlights the Infection Processes of Two Amoebophrya Strains
Article Snippet: Total RNA was quantified with RNA-specific fluorimetric quantitation on a Qubit 2.0 Fluorometer using Qubit RNA HS Assay (ThermoFisher Scientific). .. RNA quality was assessed by capillary electrophoresis on an Agilent Bioanalyzer, using the RNA 6000 Pico LabChip kit (Agilent Technologies, Santa Clara, CA). .. RNA-Seq library preparations were carried out from 1 to 4 μg total RNA using the TruSeq Stranded mRNA kit (Illumina, San Diego, CA) and the manufacturer's protocol, which allows mRNA strand orientation (sequence reads occur in the same orientation as anti-sense RNA).

Microarray:

Article Title: Microarray data of transcriptome shifts in blood cell subsets during S1P receptor modulator therapy
Article Snippet: The RNA quality was controlled using standardised operating procedures (SOPs) at the Core Facility for Microarray Analysis of the University of Rostock. .. The integrity of the RNA samples was checked using an Agilent Bioanalyzer 2100 with the RNA 6000 Pico LabChip kit (both from Agilent Technologies, Waldbronn, Germany).

Article Title: MicroRNA-221-3p, a TWIST2 target, promotes cervical cancer metastasis by directly targeting THBS2
Article Snippet: Paragraph title: miRNAs microarray ... The quality and quantity of RNA samples were assessed by a 2100 Bioanalyzer using the RNA 6000 Pico LabChip kit (Agilent Technologies, Santa Clara, CA).

Article Title: Up-Regulation of MicroRNA-145 Associates with Lymph Node Metastasis in Colorectal Cancer
Article Snippet: Paragraph title: Microarray analysis ... The quality and quantity of RNA samples were assessed by a 2100 Bioanalyzer using the RNA 6000 Pico LabChip kit (Agilent Technologies, Santa Clara, CA).

Article Title: miR-29c is downregulated in renal interstitial fibrosis in humans and rats and restored by HIF-? activation
Article Snippet: For microRNA microarray experiments, total RNA was extracted from the tissue samples using a mirVana miRNA isolation kit (Applied Biosystems, Foster City, CA). .. The quality check of RNA was performed using a 2100 Bioanalyzer with an RNA 6000 Pico LabChip kit (Agilent Technologies, Santa Clara, CA).

Article Title: Gene Expression Profiling of Endoplasmic Reticulum Stress in Hepatitis C Virus-Containing Cells Treated with an Inhibitor of Protein Disulfide Isomerases
Article Snippet: Paragraph title: Total RNA Preparation for Microarray Experiments ... The RNA was quantified using the NanoDrop ND-1000 spectrophotometer, and the quality of the RNA was verified using the RNA 6000 Pico LabChip kit (Agilent Technologies, Mississauga, Ontario).

Incubation:

Article Title: A rice Serine/Threonine receptor-like kinase regulates arbuscular mycorrhizal symbiosis at the peri-arbuscular membrane
Article Snippet: The sample was vigorously mixed and incubated at 56 °C for 5 min to facilitate cell-wall disruption. .. In addition, the integrity of RNA molecules was determined by utilization of an RNA 6000 Pico LabChip Kit and Bioanalyzer 2100 (Agilent Technologies, ).

Article Title: A rice Serine/Threonine receptor-like kinase regulates arbuscular mycorrhizal symbiosis at the peri-arbuscular membrane
Article Snippet: The sample was vigorously mixed and incubated at 56 °C for 5 min to facilitate cell-wall disruption. .. In addition, the integrity of RNA molecules was determined by utilization of an RNA 6000 Pico LabChip Kit and Bioanalyzer 2100 (Agilent Technologies, http://www.agilent.com ).

Article Title: Comparative Time-Scale Gene Expression Analysis Highlights the Infection Processes of Two Amoebophrya Strains
Article Snippet: After two rounds of 30 min incubation at 37°C, the reaction mixture was purified with the RNA Clean and Concentrator-5 kit (ZymoResearch) following the procedure described for retention of > 17 nt RNA fragments. .. RNA quality was assessed by capillary electrophoresis on an Agilent Bioanalyzer, using the RNA 6000 Pico LabChip kit (Agilent Technologies, Santa Clara, CA).

Formalin-fixed Paraffin-Embedded:

Article Title: miR-29c is downregulated in renal interstitial fibrosis in humans and rats and restored by HIF-? activation
Article Snippet: Total RNA from formalin-fixed and paraffin-embedded (FFPE) tissue was extracted by using a RecoverALL total nucleic acid isolation kit (Ambion, Austin, TX) according to the manufacturer's protocol. .. The quality check of RNA was performed using a 2100 Bioanalyzer with an RNA 6000 Pico LabChip kit (Agilent Technologies, Santa Clara, CA).

Expressing:

Article Title: MicroRNA-221-3p, a TWIST2 target, promotes cervical cancer metastasis by directly targeting THBS2
Article Snippet: Tissues (six CC tissues derived from stage I and II, respectively) and cells (SiHa, SiHa-shtw2, SiHa-tw2 cells) were collected and the expression profiles of miRNAs were determined using Agilent miRNA microarray 21.0. .. The quality and quantity of RNA samples were assessed by a 2100 Bioanalyzer using the RNA 6000 Pico LabChip kit (Agilent Technologies, Santa Clara, CA).

Article Title: Up-Regulation of MicroRNA-145 Associates with Lymph Node Metastasis in Colorectal Cancer
Article Snippet: Eight primary colorectal cancer tissues derived from stage II–III colorectal cancer patients with (n = 4) or without (n = 4) lymph node metastasis were collected and the expression profiles of miRNA were determined using Agilent miRNA microarray. .. The quality and quantity of RNA samples were assessed by a 2100 Bioanalyzer using the RNA 6000 Pico LabChip kit (Agilent Technologies, Santa Clara, CA).

Article Title: A gene expression signature that correlates with CD8+T cell expansion in acute Epstein Barr virus infection
Article Snippet: Paragraph title: Sample preparation for gene expression ... All samples were analyzed for RNA integrity on the RNA 6000 Pico Labchip kit (Agilent Technologies, Palo Alto, CA).

Article Title: Genomic and Metabolic Studies of the Impact of Probiotics on a Model Gut Symbiont and HostHow Bacterial Communities Expand Functional RepertoiresIn the Gut's Microbial Community, One Plus One Equals Many (Effects)
Article Snippet: Paragraph title: LCM and expression profiling of mouse cecal epithelium. ... RNA quality was assessed on a 2100 Bioanalyzer using an RNA 6000 Pico LabChip kit (Agilent Technologies).

Derivative Assay:

Article Title: MicroRNA-221-3p, a TWIST2 target, promotes cervical cancer metastasis by directly targeting THBS2
Article Snippet: Tissues (six CC tissues derived from stage I and II, respectively) and cells (SiHa, SiHa-shtw2, SiHa-tw2 cells) were collected and the expression profiles of miRNAs were determined using Agilent miRNA microarray 21.0. .. The quality and quantity of RNA samples were assessed by a 2100 Bioanalyzer using the RNA 6000 Pico LabChip kit (Agilent Technologies, Santa Clara, CA).

Article Title: Up-Regulation of MicroRNA-145 Associates with Lymph Node Metastasis in Colorectal Cancer
Article Snippet: Eight primary colorectal cancer tissues derived from stage II–III colorectal cancer patients with (n = 4) or without (n = 4) lymph node metastasis were collected and the expression profiles of miRNA were determined using Agilent miRNA microarray. .. The quality and quantity of RNA samples were assessed by a 2100 Bioanalyzer using the RNA 6000 Pico LabChip kit (Agilent Technologies, Santa Clara, CA).

Hybridization:

Article Title: Up-Regulation of MicroRNA-145 Associates with Lymph Node Metastasis in Colorectal Cancer
Article Snippet: The quality and quantity of RNA samples were assessed by a 2100 Bioanalyzer using the RNA 6000 Pico LabChip kit (Agilent Technologies, Santa Clara, CA). .. The microarray contains probes for 851 human miRNAs from the Sanger database v.12.0.

Article Title: A gene expression signature that correlates with CD8+T cell expansion in acute Epstein Barr virus infection
Article Snippet: All samples were analyzed for RNA integrity on the RNA 6000 Pico Labchip kit (Agilent Technologies, Palo Alto, CA). .. Data were extracted using Expression Console software.

Generated:

Article Title: Comparative Time-Scale Gene Expression Analysis Highlights the Infection Processes of Two Amoebophrya Strains
Article Snippet: RNA quality was assessed by capillary electrophoresis on an Agilent Bioanalyzer, using the RNA 6000 Pico LabChip kit (Agilent Technologies, Santa Clara, CA). .. In short, poly(A)+ RNA was selected with oligo(dT) beads, chemically fragmented and converted into single-stranded cDNA using random hexamer priming.

Inhibition:

Article Title: Microarray data of transcriptome shifts in blood cell subsets during S1P receptor modulator therapy
Article Snippet: The integrity of the RNA samples was checked using an Agilent Bioanalyzer 2100 with the RNA 6000 Pico LabChip kit (both from Agilent Technologies, Waldbronn, Germany). .. RINs between 8 and 10 indicate optimal RNA quality for microarray experiments.

Sequencing:

Article Title: Comparative Time-Scale Gene Expression Analysis Highlights the Infection Processes of Two Amoebophrya Strains
Article Snippet: Paragraph title: Transcriptome sequencing ... RNA quality was assessed by capillary electrophoresis on an Agilent Bioanalyzer, using the RNA 6000 Pico LabChip kit (Agilent Technologies, Santa Clara, CA).

Article Title: nextPARS: parallel probing of RNA structures in Illumina
Article Snippet: In this way, we tested different dilutions of both enzymes and checked their bioanalyzer profile with the RNA 6000 Pico LabChip Kit (Agilent). .. This served to assess the digestion pattern and confirm that RNA samples were not completely digested or not digested at all ( ).

Quantitation Assay:

Article Title: Comparative Time-Scale Gene Expression Analysis Highlights the Infection Processes of Two Amoebophrya Strains
Article Snippet: Total RNA was quantified with RNA-specific fluorimetric quantitation on a Qubit 2.0 Fluorometer using Qubit RNA HS Assay (ThermoFisher Scientific). .. RNA quality was assessed by capillary electrophoresis on an Agilent Bioanalyzer, using the RNA 6000 Pico LabChip kit (Agilent Technologies, Santa Clara, CA).

DNA Extraction:

Article Title: Characterization of bovine embryos cultured under conditions appropriate for sustaining human naïve pluripotency
Article Snippet: Paragraph title: ICM collection and RNA or DNA isolation ... A Bioanalyzer 2100 and the RNA 6000 Pico LabChip kit (Agilent Technologies, Amstelveen, The Netherlands) were used for assessment of total RNA quality and quantity per manufacturer’s instructions.

Radioactivity:

Article Title: nextPARS: parallel probing of RNA structures in Illumina
Article Snippet: The authors suggested that the titration of nucleases could be done radiolabeling the RNA molecules, digesting them with different enzymatic dilutions, running them on a gel and quantifying the percentage of full-length RNA before and after the digestion. .. In this way, we tested different dilutions of both enzymes and checked their bioanalyzer profile with the RNA 6000 Pico LabChip Kit (Agilent).

RNA Sequencing Assay:

Article Title: Deciphering the complex role of thrombospondin-1 in glioblastoma development
Article Snippet: Paragraph title: Laser capture microdissection and RNA-sequencing ... The integrity of the RNA was checked by capillary electrophoresis using the RNA 6000 Pico Labchip kit and the Bioanalyser 2100 (Agilent Technologies, Massy, France), and quantity was estimated using a Nanodrop 1000 (Thermo Scientific, Waltham, USA).

RNA HS Assay:

Article Title: Comparative Time-Scale Gene Expression Analysis Highlights the Infection Processes of Two Amoebophrya Strains
Article Snippet: Total RNA was quantified with RNA-specific fluorimetric quantitation on a Qubit 2.0 Fluorometer using Qubit RNA HS Assay (ThermoFisher Scientific). .. RNA quality was assessed by capillary electrophoresis on an Agilent Bioanalyzer, using the RNA 6000 Pico LabChip kit (Agilent Technologies, Santa Clara, CA).

Isolation:

Article Title: Characterization of bovine embryos cultured under conditions appropriate for sustaining human naïve pluripotency
Article Snippet: RNA isolation and on column DNA digestion was performed using an RNA micro kit (Qiagen) according to manufacturer’s protocol. .. A Bioanalyzer 2100 and the RNA 6000 Pico LabChip kit (Agilent Technologies, Amstelveen, The Netherlands) were used for assessment of total RNA quality and quantity per manufacturer’s instructions.

Article Title: MicroRNA-221-3p, a TWIST2 target, promotes cervical cancer metastasis by directly targeting THBS2
Article Snippet: In brief, total RNA was extracted from cells or tissues samples using the miRVana miRNA Isolation Kit (Ambion Inc., TX, USA). .. The quality and quantity of RNA samples were assessed by a 2100 Bioanalyzer using the RNA 6000 Pico LabChip kit (Agilent Technologies, Santa Clara, CA).

Article Title: The Expression of miR-375 Is Associated with Carcinogenesis in Three Subtypes of Lung Cancer
Article Snippet: Paragraph title: RNA isolation ... The quality control of RNA was performed by a 2100 Bioanalyzer using the RNA 6000 Pico LabChip kit (Agilent Technologies, Santa Clara, CA).

Article Title: A rice Serine/Threonine receptor-like kinase regulates arbuscular mycorrhizal symbiosis at the peri-arbuscular membrane
Article Snippet: At this stage, the lysate was either stored at −80 °C or further processed for RNA isolation. .. In addition, the integrity of RNA molecules was determined by utilization of an RNA 6000 Pico LabChip Kit and Bioanalyzer 2100 (Agilent Technologies, ).

Article Title: A mRNA landscape of bovine embryos after standard and MAPK-inhibited culture conditions: a comparative analysis
Article Snippet: Paragraph title: RNA isolation ... Total RNA quality and quantity assessment was performed by micro-electrophoresis on a Bioanalyzer 2100 using the RNA 6000 Pico LabChip kit (Agilent Technologies, Amstelveen, The Netherlands) according to manufacturer’s instructions.

Article Title: Up-Regulation of MicroRNA-145 Associates with Lymph Node Metastasis in Colorectal Cancer
Article Snippet: Briefly, total RNA was extracted from tumor samples using the miRVana miRNA Isolation Kit (Ambion Inc., TX, USA). .. The quality and quantity of RNA samples were assessed by a 2100 Bioanalyzer using the RNA 6000 Pico LabChip kit (Agilent Technologies, Santa Clara, CA).

Article Title: Expression of pro-apoptotic markers is increased along the osteochondral junction in naturally occurring osteochondrosis
Article Snippet: Paragraph title: 2.4. RNA isolation ... RNA quality control was performed using an Agilent 2100 Bioanalyzer and the RNA 6000 Pico LabChip kit (Agilent Technologies, Santa Clara, CA, USA).

Article Title: miR-29c is downregulated in renal interstitial fibrosis in humans and rats and restored by HIF-? activation
Article Snippet: Paragraph title: RNA isolation. ... The quality check of RNA was performed using a 2100 Bioanalyzer with an RNA 6000 Pico LabChip kit (Agilent Technologies, Santa Clara, CA).

Article Title: Genome-Wide Screening of Genes Regulated by DNA Methylation in Colon Cancer Development
Article Snippet: Total RNA was extracted from the microdissected tissue samples and from the treated HT-29 cells using RNeasy Micro Kit (Qiagen) according to the manufacturer’s instruction. .. The quality of the isolated nucleic acid was measured by microcapillary electrophoresis system using RNA 6000 Pico LabChip kit (Agilent BioAnalyzer 2100). .. For the microarray analysis 5–50 ng total RNA was used from the samples with RIN (RNA Integrity Number) between 7–10.

Article Title: A rice Serine/Threonine receptor-like kinase regulates arbuscular mycorrhizal symbiosis at the peri-arbuscular membrane
Article Snippet: At this stage, the lysate was either stored at −80 °C or further processed for RNA isolation. .. In addition, the integrity of RNA molecules was determined by utilization of an RNA 6000 Pico LabChip Kit and Bioanalyzer 2100 (Agilent Technologies, http://www.agilent.com ).

Article Title: A gene expression signature that correlates with CD8+T cell expansion in acute Epstein Barr virus infection
Article Snippet: CD8+T Cells were isolated from thawed PBMC using a MACSR CD8+ T Cell Isolation Kit (Miltenyi Biotech, Cambridge MA). .. All samples were analyzed for RNA integrity on the RNA 6000 Pico Labchip kit (Agilent Technologies, Palo Alto, CA).

Article Title: Genomic and Metabolic Studies of the Impact of Probiotics on a Model Gut Symbiont and HostHow Bacterial Communities Expand Functional RepertoiresIn the Gut's Microbial Community, One Plus One Equals Many (Effects)
Article Snippet: Total RNA was purified from microdissected epithelial cells using the PicoPure RNA isolation kit (Arcturus) with on-column DNase (Qiagen) treatment. .. RNA quality was assessed on a 2100 Bioanalyzer using an RNA 6000 Pico LabChip kit (Agilent Technologies).

Article Title: Cross-Species Comparison of Human and Mouse Intestinal Polyps Reveals Conserved Mechanisms in Adenomatous Polyposis Coli (APC)-Driven Tumorigenesis
Article Snippet: RNA was isolated from the LCM samples by Mini RNeasy columns (QIAGEN, Valencia, CA), according to the manufacturer’s instructions, including a DNase step on the column. .. Quality of the isolated RNA was checked with RNA 6000 Pico LabChip kit (Agilent Technologies, Palo Alto, CA). .. Each RNA sample that passed the quality controls was linearly amplified with two rounds of amplification using the MessageAmp kit (Ambion, Huntingdon, UK), according to the manufacturer’s protocol.

Microscopy:

Article Title: Characterization of bovine embryos cultured under conditions appropriate for sustaining human naïve pluripotency
Article Snippet: In short, blastocysts were placed in wash buffer and sharpened tungsten needles were used to separate the ICM from the TE under a stereo microscope. .. A Bioanalyzer 2100 and the RNA 6000 Pico LabChip kit (Agilent Technologies, Amstelveen, The Netherlands) were used for assessment of total RNA quality and quantity per manufacturer’s instructions.

Article Title: Cross-Species Comparison of Human and Mouse Intestinal Polyps Reveals Conserved Mechanisms in Adenomatous Polyposis Coli (APC)-Driven Tumorigenesis
Article Snippet: In short, 10-μm cryosections were mounted on microscope slides with a polyethylene naphthalate membrane and stained by H & E to allow histological identification of the desired normal and tumor cells. .. Quality of the isolated RNA was checked with RNA 6000 Pico LabChip kit (Agilent Technologies, Palo Alto, CA).

Purification:

Article Title: Enhanced molecular analyses by combination of the HOPE-technique and laser microdissection
Article Snippet: Briefly, the cells were dissolved in 350 μl of RLT lysis buffer, treated with DNaseI after the first washing step according to the manual and finally the purified RNA was eluted in 12 μl of RNase free water. .. Of each RNA sample 1 μl was tested in an Agilent Bioanalyzer 2100 using the RNA 6000 Pico LabChip kit (Agilent, Waldbronn, Germany, #5065-4473) to determine the RNA integrity.

Article Title: A gene expression signature that correlates with CD8+T cell expansion in acute Epstein Barr virus infection
Article Snippet: Purified CD8+T Cells were either used immediately or re-suspended in RNA Later (Life Technologies, Inc, Grand Island NY) and stored at −40°C. .. All samples were analyzed for RNA integrity on the RNA 6000 Pico Labchip kit (Agilent Technologies, Palo Alto, CA).

Article Title: Comparative Time-Scale Gene Expression Analysis Highlights the Infection Processes of Two Amoebophrya Strains
Article Snippet: After two rounds of 30 min incubation at 37°C, the reaction mixture was purified with the RNA Clean and Concentrator-5 kit (ZymoResearch) following the procedure described for retention of > 17 nt RNA fragments. .. RNA quality was assessed by capillary electrophoresis on an Agilent Bioanalyzer, using the RNA 6000 Pico LabChip kit (Agilent Technologies, Santa Clara, CA).

Article Title: Genomic and Metabolic Studies of the Impact of Probiotics on a Model Gut Symbiont and HostHow Bacterial Communities Expand Functional RepertoiresIn the Gut's Microbial Community, One Plus One Equals Many (Effects)
Article Snippet: Total RNA was purified from microdissected epithelial cells using the PicoPure RNA isolation kit (Arcturus) with on-column DNase (Qiagen) treatment. .. RNA quality was assessed on a 2100 Bioanalyzer using an RNA 6000 Pico LabChip kit (Agilent Technologies).

Labeling:

Article Title: Up-Regulation of MicroRNA-145 Associates with Lymph Node Metastasis in Colorectal Cancer
Article Snippet: The quality and quantity of RNA samples were assessed by a 2100 Bioanalyzer using the RNA 6000 Pico LabChip kit (Agilent Technologies, Santa Clara, CA). .. The microarray contains probes for 851 human miRNAs from the Sanger database v.12.0.

Lysis:

Article Title: Enhanced molecular analyses by combination of the HOPE-technique and laser microdissection
Article Snippet: Briefly, the cells were dissolved in 350 μl of RLT lysis buffer, treated with DNaseI after the first washing step according to the manual and finally the purified RNA was eluted in 12 μl of RNase free water. .. Of each RNA sample 1 μl was tested in an Agilent Bioanalyzer 2100 using the RNA 6000 Pico LabChip kit (Agilent, Waldbronn, Germany, #5065-4473) to determine the RNA integrity.

Titration:

Article Title: nextPARS: parallel probing of RNA structures in Illumina
Article Snippet: The authors suggested that the titration of nucleases could be done radiolabeling the RNA molecules, digesting them with different enzymatic dilutions, running them on a gel and quantifying the percentage of full-length RNA before and after the digestion. .. In this way, we tested different dilutions of both enzymes and checked their bioanalyzer profile with the RNA 6000 Pico LabChip Kit (Agilent).

Chromatin Immunoprecipitation:

Article Title: A gene expression signature that correlates with CD8+T cell expansion in acute Epstein Barr virus infection
Article Snippet: All samples were analyzed for RNA integrity on the RNA 6000 Pico Labchip kit (Agilent Technologies, Palo Alto, CA). .. Data were extracted using Expression Console software.

Software:

Article Title: MicroRNA-221-3p, a TWIST2 target, promotes cervical cancer metastasis by directly targeting THBS2
Article Snippet: The quality and quantity of RNA samples were assessed by a 2100 Bioanalyzer using the RNA 6000 Pico LabChip kit (Agilent Technologies, Santa Clara, CA). .. The miRNA microarray was carried out at Shanghai Biotechnology Corporation (Shanghai, China).

Article Title: A gene expression signature that correlates with CD8+T cell expansion in acute Epstein Barr virus infection
Article Snippet: All samples were analyzed for RNA integrity on the RNA 6000 Pico Labchip kit (Agilent Technologies, Palo Alto, CA). .. All samples were analyzed for RNA integrity on the RNA 6000 Pico Labchip kit (Agilent Technologies, Palo Alto, CA).

RNA Extraction:

Article Title: Enhanced molecular analyses by combination of the HOPE-technique and laser microdissection
Article Snippet: Paragraph title: RNA extraction ... Of each RNA sample 1 μl was tested in an Agilent Bioanalyzer 2100 using the RNA 6000 Pico LabChip kit (Agilent, Waldbronn, Germany, #5065-4473) to determine the RNA integrity.

Sample Prep:

Article Title: A gene expression signature that correlates with CD8+T cell expansion in acute Epstein Barr virus infection
Article Snippet: Paragraph title: Sample preparation for gene expression ... All samples were analyzed for RNA integrity on the RNA 6000 Pico Labchip kit (Agilent Technologies, Palo Alto, CA).

Article Title: Cross-Species Comparison of Human and Mouse Intestinal Polyps Reveals Conserved Mechanisms in Adenomatous Polyposis Coli (APC)-Driven Tumorigenesis
Article Snippet: Sample preparation and laser capture microdissection (LCM) were performed as previously described using a PALM MicroBeam microscope system (P.A.L.M. .. Quality of the isolated RNA was checked with RNA 6000 Pico LabChip kit (Agilent Technologies, Palo Alto, CA).

Laser Capture Microdissection:

Article Title: A rice Serine/Threonine receptor-like kinase regulates arbuscular mycorrhizal symbiosis at the peri-arbuscular membrane
Article Snippet: Paragraph title: Laser capture microdissection ... In addition, the integrity of RNA molecules was determined by utilization of an RNA 6000 Pico LabChip Kit and Bioanalyzer 2100 (Agilent Technologies, ).

Article Title: Deciphering the complex role of thrombospondin-1 in glioblastoma development
Article Snippet: Paragraph title: Laser capture microdissection and RNA-sequencing ... The integrity of the RNA was checked by capillary electrophoresis using the RNA 6000 Pico Labchip kit and the Bioanalyser 2100 (Agilent Technologies, Massy, France), and quantity was estimated using a Nanodrop 1000 (Thermo Scientific, Waltham, USA).

Article Title: Expression of pro-apoptotic markers is increased along the osteochondral junction in naturally occurring osteochondrosis
Article Snippet: PicoPure RNA Isolation Kit (Arcturus Bioscience, Mountainview, CA, USA) was used for RNA isolation of LCM samples with slight modifications. .. RNA quality control was performed using an Agilent 2100 Bioanalyzer and the RNA 6000 Pico LabChip kit (Agilent Technologies, Santa Clara, CA, USA).

Article Title: Genomic and Metabolic Studies of the Impact of Probiotics on a Model Gut Symbiont and HostHow Bacterial Communities Expand Functional RepertoiresIn the Gut's Microbial Community, One Plus One Equals Many (Effects)
Article Snippet: Paragraph title: LCM and expression profiling of mouse cecal epithelium. ... RNA quality was assessed on a 2100 Bioanalyzer using an RNA 6000 Pico LabChip kit (Agilent Technologies).

Article Title: Cross-Species Comparison of Human and Mouse Intestinal Polyps Reveals Conserved Mechanisms in Adenomatous Polyposis Coli (APC)-Driven Tumorigenesis
Article Snippet: Paragraph title: Laser Capture Microdissection and RNA Isolation ... Quality of the isolated RNA was checked with RNA 6000 Pico LabChip kit (Agilent Technologies, Palo Alto, CA).

Random Hexamer Labeling:

Article Title: Comparative Time-Scale Gene Expression Analysis Highlights the Infection Processes of Two Amoebophrya Strains
Article Snippet: RNA quality was assessed by capillary electrophoresis on an Agilent Bioanalyzer, using the RNA 6000 Pico LabChip kit (Agilent Technologies, Santa Clara, CA). .. RNA-Seq library preparations were carried out from 1 to 4 μg total RNA using the TruSeq Stranded mRNA kit (Illumina, San Diego, CA) and the manufacturer's protocol, which allows mRNA strand orientation (sequence reads occur in the same orientation as anti-sense RNA).

Spectrophotometry:

Article Title: Enhanced molecular analyses by combination of the HOPE-technique and laser microdissection
Article Snippet: Spectrophotometry was performed for testing concentration and purity of RNA, which revealed well-preserved RNA of high concentration throughout the samples. .. Of each RNA sample 1 μl was tested in an Agilent Bioanalyzer 2100 using the RNA 6000 Pico LabChip kit (Agilent, Waldbronn, Germany, #5065-4473) to determine the RNA integrity.

Article Title: The Expression of miR-375 Is Associated with Carcinogenesis in Three Subtypes of Lung Cancer
Article Snippet: The concentration was quantified by NanoDrop 1000 Spectrophotometer (NanoDrop Technologies, Waltham, MA). .. The quality control of RNA was performed by a 2100 Bioanalyzer using the RNA 6000 Pico LabChip kit (Agilent Technologies, Santa Clara, CA).

Article Title: miR-29c is downregulated in renal interstitial fibrosis in humans and rats and restored by HIF-? activation
Article Snippet: The concentration was quantified by a NanoDrop 1000 Spectrophotometer (NanoDrop Technologies, Waltham, MA). .. The quality check of RNA was performed using a 2100 Bioanalyzer with an RNA 6000 Pico LabChip kit (Agilent Technologies, Santa Clara, CA).

Article Title: Gene Expression Profiling of Endoplasmic Reticulum Stress in Hepatitis C Virus-Containing Cells Treated with an Inhibitor of Protein Disulfide Isomerases
Article Snippet: Total RNA was prepared using the RNeasy Mini kit (Qiagen, Mississauga, Ontario) as per the manufacturer’s protocols. .. The RNA was quantified using the NanoDrop ND-1000 spectrophotometer, and the quality of the RNA was verified using the RNA 6000 Pico LabChip kit (Agilent Technologies, Mississauga, Ontario). .. Cellular RNA samples were hybridized to high-density oligonucleotide arrays (Affymetrix GeneChip HG-U133 Plus2).

Concentration Assay:

Article Title: Enhanced molecular analyses by combination of the HOPE-technique and laser microdissection
Article Snippet: Spectrophotometry was performed for testing concentration and purity of RNA, which revealed well-preserved RNA of high concentration throughout the samples. .. Of each RNA sample 1 μl was tested in an Agilent Bioanalyzer 2100 using the RNA 6000 Pico LabChip kit (Agilent, Waldbronn, Germany, #5065-4473) to determine the RNA integrity.

Article Title: The Expression of miR-375 Is Associated with Carcinogenesis in Three Subtypes of Lung Cancer
Article Snippet: The concentration was quantified by NanoDrop 1000 Spectrophotometer (NanoDrop Technologies, Waltham, MA). .. The quality control of RNA was performed by a 2100 Bioanalyzer using the RNA 6000 Pico LabChip kit (Agilent Technologies, Santa Clara, CA).

Article Title: A rice Serine/Threonine receptor-like kinase regulates arbuscular mycorrhizal symbiosis at the peri-arbuscular membrane
Article Snippet: The RNA concentration was determined using NanoDrop ND-1000 (NanoDrop Products, ). .. In addition, the integrity of RNA molecules was determined by utilization of an RNA 6000 Pico LabChip Kit and Bioanalyzer 2100 (Agilent Technologies, ).

Article Title: miR-29c is downregulated in renal interstitial fibrosis in humans and rats and restored by HIF-? activation
Article Snippet: The concentration was quantified by a NanoDrop 1000 Spectrophotometer (NanoDrop Technologies, Waltham, MA). .. The quality check of RNA was performed using a 2100 Bioanalyzer with an RNA 6000 Pico LabChip kit (Agilent Technologies, Santa Clara, CA).

Article Title: A rice Serine/Threonine receptor-like kinase regulates arbuscular mycorrhizal symbiosis at the peri-arbuscular membrane
Article Snippet: The RNA concentration was determined using NanoDrop ND-1000 (NanoDrop Products, http://www.nanodrop.com ). .. In addition, the integrity of RNA molecules was determined by utilization of an RNA 6000 Pico LabChip Kit and Bioanalyzer 2100 (Agilent Technologies, http://www.agilent.com ).

Article Title: nextPARS: parallel probing of RNA structures in Illumina
Article Snippet: In this way, we tested different dilutions of both enzymes and checked their bioanalyzer profile with the RNA 6000 Pico LabChip Kit (Agilent). .. Then, rather than measuring the percentage of undigested/digested RNA molecules as in previous studies developing the PARS technique , we directly analyzed the sequencing results of different samples treated with distinct enzyme concentrations, as well as a nondigested sample.

Staining:

Article Title: Cross-Species Comparison of Human and Mouse Intestinal Polyps Reveals Conserved Mechanisms in Adenomatous Polyposis Coli (APC)-Driven Tumorigenesis
Article Snippet: In short, 10-μm cryosections were mounted on microscope slides with a polyethylene naphthalate membrane and stained by H & E to allow histological identification of the desired normal and tumor cells. .. Quality of the isolated RNA was checked with RNA 6000 Pico LabChip kit (Agilent Technologies, Palo Alto, CA).

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    Agilent technologies rna 6000 pico labchip kit
    Integrity of the RNA samples. Each bar depicts the arithmetic mean of the RNA integrity numbers (RIN) measured for the 10 whole RNA samples, which were used for the microarray hybridisation per cell population and per time point. The error bars indicate standard deviations. This quality control was carried out using the Agilent Bioanalyzer 2100 and the RNA 6000 Pico <t>LabChip</t> kit (Agilent Technologies, Waldbronn, Germany).
    Rna 6000 Pico Labchip Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 97/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Integrity of the RNA samples. Each bar depicts the arithmetic mean of the RNA integrity numbers (RIN) measured for the 10 whole RNA samples, which were used for the microarray hybridisation per cell population and per time point. The error bars indicate standard deviations. This quality control was carried out using the Agilent Bioanalyzer 2100 and the RNA 6000 Pico LabChip kit (Agilent Technologies, Waldbronn, Germany).

    Journal: Scientific Data

    Article Title: Microarray data of transcriptome shifts in blood cell subsets during S1P receptor modulator therapy

    doi: 10.1038/sdata.2018.145

    Figure Lengend Snippet: Integrity of the RNA samples. Each bar depicts the arithmetic mean of the RNA integrity numbers (RIN) measured for the 10 whole RNA samples, which were used for the microarray hybridisation per cell population and per time point. The error bars indicate standard deviations. This quality control was carried out using the Agilent Bioanalyzer 2100 and the RNA 6000 Pico LabChip kit (Agilent Technologies, Waldbronn, Germany).

    Article Snippet: The integrity of the RNA samples was checked using an Agilent Bioanalyzer 2100 with the RNA 6000 Pico LabChip kit (both from Agilent Technologies, Waldbronn, Germany).

    Techniques: Microarray, Hybridization

    Results of RNA-integrity-testing by a Agilent Bioanalyzer 2100 using the RNA 6000 Pico LabChip kit. Lane 1 and 2 are HOPE-fixed materials and lane 3 is FFPE.

    Journal: Diagnostic Pathology

    Article Title: Enhanced molecular analyses by combination of the HOPE-technique and laser microdissection

    doi: 10.1186/1746-1596-1-2

    Figure Lengend Snippet: Results of RNA-integrity-testing by a Agilent Bioanalyzer 2100 using the RNA 6000 Pico LabChip kit. Lane 1 and 2 are HOPE-fixed materials and lane 3 is FFPE.

    Article Snippet: Of each RNA sample 1 μl was tested in an Agilent Bioanalyzer 2100 using the RNA 6000 Pico LabChip kit (Agilent, Waldbronn, Germany, #5065-4473) to determine the RNA integrity.

    Techniques: Formalin-fixed Paraffin-Embedded