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Agilent technologies rna 6000 pico labchip kit
Results of RNA-integrity-testing by a Agilent Bioanalyzer 2100 using the <t>RNA</t> 6000 Pico <t>LabChip</t> kit. Lane 1 and 2 are HOPE-fixed materials and lane 3 is FFPE.
Rna 6000 Pico Labchip Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 91/100, based on 88 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rna 6000 pico labchip kit/product/Agilent technologies
Average 91 stars, based on 88 article reviews
Price from $9.99 to $1999.99
rna 6000 pico labchip kit - by Bioz Stars, 2020-09
91/100 stars

Images

1) Product Images from "Enhanced molecular analyses by combination of the HOPE-technique and laser microdissection"

Article Title: Enhanced molecular analyses by combination of the HOPE-technique and laser microdissection

Journal: Diagnostic Pathology

doi: 10.1186/1746-1596-1-2

Results of RNA-integrity-testing by a Agilent Bioanalyzer 2100 using the RNA 6000 Pico LabChip kit. Lane 1 and 2 are HOPE-fixed materials and lane 3 is FFPE.
Figure Legend Snippet: Results of RNA-integrity-testing by a Agilent Bioanalyzer 2100 using the RNA 6000 Pico LabChip kit. Lane 1 and 2 are HOPE-fixed materials and lane 3 is FFPE.

Techniques Used: Formalin-fixed Paraffin-Embedded

2) Product Images from "Microarray data of transcriptome shifts in blood cell subsets during S1P receptor modulator therapy"

Article Title: Microarray data of transcriptome shifts in blood cell subsets during S1P receptor modulator therapy

Journal: Scientific Data

doi: 10.1038/sdata.2018.145

Integrity of the RNA samples. Each bar depicts the arithmetic mean of the RNA integrity numbers (RIN) measured for the 10 whole RNA samples, which were used for the microarray hybridisation per cell population and per time point. The error bars indicate standard deviations. This quality control was carried out using the Agilent Bioanalyzer 2100 and the RNA 6000 Pico LabChip kit (Agilent Technologies, Waldbronn, Germany).
Figure Legend Snippet: Integrity of the RNA samples. Each bar depicts the arithmetic mean of the RNA integrity numbers (RIN) measured for the 10 whole RNA samples, which were used for the microarray hybridisation per cell population and per time point. The error bars indicate standard deviations. This quality control was carried out using the Agilent Bioanalyzer 2100 and the RNA 6000 Pico LabChip kit (Agilent Technologies, Waldbronn, Germany).

Techniques Used: Microarray, Hybridization

3) Product Images from "Microarray data of transcriptome shifts in blood cell subsets during S1P receptor modulator therapy"

Article Title: Microarray data of transcriptome shifts in blood cell subsets during S1P receptor modulator therapy

Journal: Scientific Data

doi: 10.1038/sdata.2018.145

Integrity of the RNA samples. Each bar depicts the arithmetic mean of the RNA integrity numbers (RIN) measured for the 10 whole RNA samples, which were used for the microarray hybridisation per cell population and per time point. The error bars indicate standard deviations. This quality control was carried out using the Agilent Bioanalyzer 2100 and the RNA 6000 Pico LabChip kit (Agilent Technologies, Waldbronn, Germany).
Figure Legend Snippet: Integrity of the RNA samples. Each bar depicts the arithmetic mean of the RNA integrity numbers (RIN) measured for the 10 whole RNA samples, which were used for the microarray hybridisation per cell population and per time point. The error bars indicate standard deviations. This quality control was carried out using the Agilent Bioanalyzer 2100 and the RNA 6000 Pico LabChip kit (Agilent Technologies, Waldbronn, Germany).

Techniques Used: Microarray, Hybridization

4) Product Images from "A metallo-beta-lactamase with both beta-lactamase and ribonuclease activity is linked with traduction in giant viruses"

Article Title: A metallo-beta-lactamase with both beta-lactamase and ribonuclease activity is linked with traduction in giant viruses

Journal: bioRxiv

doi: 10.1101/819797

Digital gel images of RNase activity of expressed Tupanvirus protein TupBlac on E. coli RNA. RNA samples (1 μg) were incubated with 15 μg of TupBlac at 30°C in the absence or presence of 10 μg/mL of sulbactam or 200 μM of ceftriaxone. Nuclease activity was visualized as digital gel images performed using the Agilent Bioanalyzer 2100 with the RNA 6000 Pico LabChip (Agilent Technologies, Palo Alto, CA). a: RNA used as substrate was from Escherichia coli; no treatment (a); buffer (b); sulbactam (c); TupBlac in the absence (d) or presence (e) of sulbactam. b: RNA used as substrate was from Escherichia coli; reactions were stopped at different times (5 min, 10 min, 30 min, 1 h and 2 h) by the addition of proteinase K (10 μg) and incubation for 1h at 37°C. The first lane corresponds to no treatment; lanes 2 to 6 to RNA treatment with TupBlac in the absence of ceftriaxone; lanes 7 to 11 to RNA treatment with TupBlac in the presence of ceftriaxone. c: nuclease activity on RNAs originating from Acanthamoeba castellanii ; no treatment (a); buffer (b); TupBlac in the absence (c, d) or presence (e) of sulbactam. d: nuclease activity on RNAs originating from bacteria that differ by the G+C-content of their genome, as indicated at the top of the digital gel image. For each RNA, three samples were analyzed: no treatment (1); treatment with TupBlac in the absence of sulbactam (2); and treatment with TupBlac in the presence of sulbactam (3).
Figure Legend Snippet: Digital gel images of RNase activity of expressed Tupanvirus protein TupBlac on E. coli RNA. RNA samples (1 μg) were incubated with 15 μg of TupBlac at 30°C in the absence or presence of 10 μg/mL of sulbactam or 200 μM of ceftriaxone. Nuclease activity was visualized as digital gel images performed using the Agilent Bioanalyzer 2100 with the RNA 6000 Pico LabChip (Agilent Technologies, Palo Alto, CA). a: RNA used as substrate was from Escherichia coli; no treatment (a); buffer (b); sulbactam (c); TupBlac in the absence (d) or presence (e) of sulbactam. b: RNA used as substrate was from Escherichia coli; reactions were stopped at different times (5 min, 10 min, 30 min, 1 h and 2 h) by the addition of proteinase K (10 μg) and incubation for 1h at 37°C. The first lane corresponds to no treatment; lanes 2 to 6 to RNA treatment with TupBlac in the absence of ceftriaxone; lanes 7 to 11 to RNA treatment with TupBlac in the presence of ceftriaxone. c: nuclease activity on RNAs originating from Acanthamoeba castellanii ; no treatment (a); buffer (b); TupBlac in the absence (c, d) or presence (e) of sulbactam. d: nuclease activity on RNAs originating from bacteria that differ by the G+C-content of their genome, as indicated at the top of the digital gel image. For each RNA, three samples were analyzed: no treatment (1); treatment with TupBlac in the absence of sulbactam (2); and treatment with TupBlac in the presence of sulbactam (3).

Techniques Used: Activity Assay, Incubation

5) Product Images from "Maintaining RNA integrity in a homogeneous population of mammary epithelial cells isolated by Laser Capture Microdissection"

Article Title: Maintaining RNA integrity in a homogeneous population of mammary epithelial cells isolated by Laser Capture Microdissection

Journal: BMC Cell Biology

doi: 10.1186/1471-2121-11-95

Impact of the freezing system on morphology of fresh mammary tissue sections and on quality of RNA extracted . Alveolar (acini) structures lined by MECs (yellow arrows) can be easily distinguished after staining mammary tissue sections with Cresyl violet AMBION. Immediately after collection, mammary tissue samples were washed in cold PBS solution, cut in cube of 3-4 mm thickness, and frozen in four different conditions: pieces of mammary tissue were either directly introduced into 1.5-ml eppendorf tubes and frozen in liquid nitrogen (A), or in cold isopentane (-80°C) using the SnapFrost™ system (C), or embedded in OCT ® contained in cryomold before to be immediately immerged in liquid nitrogen (B) or in cold isopentane (D). RNA quality (RIN) which was estimated by RNA 6000 Pico LabChip kit and Agilent 2100 Bioanalyzer, was identical (ranging between 8.5 and 9.5) whatever the freezing procedure, as illustrated in the electrophoreris profiles. Some large blisters (red arrows on Figure A and B) appear however in biopsy flash frozen in liquid nitrogen, mainly without cryoprotector, and morphological details are better seen on tissues frozen using the SnapFrost™ system (Magnification: ×60). The green arrow indicates the thermoplastic film stuck on epithelial cells to be captured.
Figure Legend Snippet: Impact of the freezing system on morphology of fresh mammary tissue sections and on quality of RNA extracted . Alveolar (acini) structures lined by MECs (yellow arrows) can be easily distinguished after staining mammary tissue sections with Cresyl violet AMBION. Immediately after collection, mammary tissue samples were washed in cold PBS solution, cut in cube of 3-4 mm thickness, and frozen in four different conditions: pieces of mammary tissue were either directly introduced into 1.5-ml eppendorf tubes and frozen in liquid nitrogen (A), or in cold isopentane (-80°C) using the SnapFrost™ system (C), or embedded in OCT ® contained in cryomold before to be immediately immerged in liquid nitrogen (B) or in cold isopentane (D). RNA quality (RIN) which was estimated by RNA 6000 Pico LabChip kit and Agilent 2100 Bioanalyzer, was identical (ranging between 8.5 and 9.5) whatever the freezing procedure, as illustrated in the electrophoreris profiles. Some large blisters (red arrows on Figure A and B) appear however in biopsy flash frozen in liquid nitrogen, mainly without cryoprotector, and morphological details are better seen on tissues frozen using the SnapFrost™ system (Magnification: ×60). The green arrow indicates the thermoplastic film stuck on epithelial cells to be captured.

Techniques Used: Staining

Related Articles

Electrophoresis:

Article Title: Maintaining RNA integrity in a homogeneous population of mammary epithelial cells isolated by Laser Capture Microdissection
Article Snippet: .. The fluorimetric method and micro-capillary electrophoresis device developed by Agilent Technologies was chosen to determine RNA concentration and quality with RNA 6000 pico LabChip Kit in the Agilent Bioanalyzer 2100 system. .. Quality was evaluated using the RNA Integrity Number (RIN) value introduced by Agilent [ ].

Isolation:

Article Title: CNGA3: A Target of Spinal Nitric Oxide/cGMP Signaling and Modulator of Inflammatory Pain Hypersensitivity
Article Snippet: .. Integrity of the isolated RNA was determined using the Agilent 2100 bioanalyzer and RNA 6000 Pico LabChip Kit (Agilent Technologies). .. Electropherograms demonstrated clear 18S and 28S rRNA peaks and no significant shift of RNA fragments to shorter migration times, indicating high RNA quality [RIN (RNA integrity number) > 7].

Incubation:

Article Title: A metallo-beta-lactamase with both beta-lactamase and ribonuclease activity is linked with traduction in giant viruses
Article Snippet: .. Enzymatic reactions were performed by incubating each polynucleotide (2 μg) with 15 μg of the expressed Tupanvirus protein TupBlac in Tris-HCl buffer 50 mM, pH 8.0, sodium chloride 0.3 M, using a final volume of 20 μL at 30°C for 2 h. After incubation, the material was loaded onto denaturing polyacrylamide gel electrophoresis (dPAGE) at 12% or analysed using the Agilent RNA 6000 Pico LabChip kit on an Agilent 2100 Bioanalyzer (Agilent Technology, Palo Alto, CA, USA). ..

Polyacrylamide Gel Electrophoresis:

Article Title: A metallo-beta-lactamase with both beta-lactamase and ribonuclease activity is linked with traduction in giant viruses
Article Snippet: .. Enzymatic reactions were performed by incubating each polynucleotide (2 μg) with 15 μg of the expressed Tupanvirus protein TupBlac in Tris-HCl buffer 50 mM, pH 8.0, sodium chloride 0.3 M, using a final volume of 20 μL at 30°C for 2 h. After incubation, the material was loaded onto denaturing polyacrylamide gel electrophoresis (dPAGE) at 12% or analysed using the Agilent RNA 6000 Pico LabChip kit on an Agilent 2100 Bioanalyzer (Agilent Technology, Palo Alto, CA, USA). ..

Concentration Assay:

Article Title: Maintaining RNA integrity in a homogeneous population of mammary epithelial cells isolated by Laser Capture Microdissection
Article Snippet: .. The fluorimetric method and micro-capillary electrophoresis device developed by Agilent Technologies was chosen to determine RNA concentration and quality with RNA 6000 pico LabChip Kit in the Agilent Bioanalyzer 2100 system. .. Quality was evaluated using the RNA Integrity Number (RIN) value introduced by Agilent [ ].

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    Agilent technologies rna 6000 pico labchip kit
    Results of RNA-integrity-testing by a Agilent Bioanalyzer 2100 using the <t>RNA</t> 6000 Pico <t>LabChip</t> kit. Lane 1 and 2 are HOPE-fixed materials and lane 3 is FFPE.
    Rna 6000 Pico Labchip Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 91/100, based on 88 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rna 6000 pico labchip kit/product/Agilent technologies
    Average 91 stars, based on 88 article reviews
    Price from $9.99 to $1999.99
    rna 6000 pico labchip kit - by Bioz Stars, 2020-09
    91/100 stars
      Buy from Supplier

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    Results of RNA-integrity-testing by a Agilent Bioanalyzer 2100 using the RNA 6000 Pico LabChip kit. Lane 1 and 2 are HOPE-fixed materials and lane 3 is FFPE.

    Journal: Diagnostic Pathology

    Article Title: Enhanced molecular analyses by combination of the HOPE-technique and laser microdissection

    doi: 10.1186/1746-1596-1-2

    Figure Lengend Snippet: Results of RNA-integrity-testing by a Agilent Bioanalyzer 2100 using the RNA 6000 Pico LabChip kit. Lane 1 and 2 are HOPE-fixed materials and lane 3 is FFPE.

    Article Snippet: Of each RNA sample 1 μl was tested in an Agilent Bioanalyzer 2100 using the RNA 6000 Pico LabChip kit (Agilent, Waldbronn, Germany, #5065-4473) to determine the RNA integrity.

    Techniques: Formalin-fixed Paraffin-Embedded

    Integrity of the RNA samples. Each bar depicts the arithmetic mean of the RNA integrity numbers (RIN) measured for the 10 whole RNA samples, which were used for the microarray hybridisation per cell population and per time point. The error bars indicate standard deviations. This quality control was carried out using the Agilent Bioanalyzer 2100 and the RNA 6000 Pico LabChip kit (Agilent Technologies, Waldbronn, Germany).

    Journal: Scientific Data

    Article Title: Microarray data of transcriptome shifts in blood cell subsets during S1P receptor modulator therapy

    doi: 10.1038/sdata.2018.145

    Figure Lengend Snippet: Integrity of the RNA samples. Each bar depicts the arithmetic mean of the RNA integrity numbers (RIN) measured for the 10 whole RNA samples, which were used for the microarray hybridisation per cell population and per time point. The error bars indicate standard deviations. This quality control was carried out using the Agilent Bioanalyzer 2100 and the RNA 6000 Pico LabChip kit (Agilent Technologies, Waldbronn, Germany).

    Article Snippet: The integrity of the RNA samples was checked using an Agilent Bioanalyzer 2100 with the RNA 6000 Pico LabChip kit (both from Agilent Technologies, Waldbronn, Germany).

    Techniques: Microarray, Hybridization

    Integrity of the RNA samples. Each bar depicts the arithmetic mean of the RNA integrity numbers (RIN) measured for the 10 whole RNA samples, which were used for the microarray hybridisation per cell population and per time point. The error bars indicate standard deviations. This quality control was carried out using the Agilent Bioanalyzer 2100 and the RNA 6000 Pico LabChip kit (Agilent Technologies, Waldbronn, Germany).

    Journal: Scientific Data

    Article Title: Microarray data of transcriptome shifts in blood cell subsets during S1P receptor modulator therapy

    doi: 10.1038/sdata.2018.145

    Figure Lengend Snippet: Integrity of the RNA samples. Each bar depicts the arithmetic mean of the RNA integrity numbers (RIN) measured for the 10 whole RNA samples, which were used for the microarray hybridisation per cell population and per time point. The error bars indicate standard deviations. This quality control was carried out using the Agilent Bioanalyzer 2100 and the RNA 6000 Pico LabChip kit (Agilent Technologies, Waldbronn, Germany).

    Article Snippet: The integrity of the RNA samples was checked using an Agilent Bioanalyzer 2100 with the RNA 6000 Pico LabChip kit (both from Agilent Technologies, Waldbronn, Germany).

    Techniques: Microarray, Hybridization

    Digital gel images of RNase activity of expressed Tupanvirus protein TupBlac on E. coli RNA. RNA samples (1 μg) were incubated with 15 μg of TupBlac at 30°C in the absence or presence of 10 μg/mL of sulbactam or 200 μM of ceftriaxone. Nuclease activity was visualized as digital gel images performed using the Agilent Bioanalyzer 2100 with the RNA 6000 Pico LabChip (Agilent Technologies, Palo Alto, CA). a: RNA used as substrate was from Escherichia coli; no treatment (a); buffer (b); sulbactam (c); TupBlac in the absence (d) or presence (e) of sulbactam. b: RNA used as substrate was from Escherichia coli; reactions were stopped at different times (5 min, 10 min, 30 min, 1 h and 2 h) by the addition of proteinase K (10 μg) and incubation for 1h at 37°C. The first lane corresponds to no treatment; lanes 2 to 6 to RNA treatment with TupBlac in the absence of ceftriaxone; lanes 7 to 11 to RNA treatment with TupBlac in the presence of ceftriaxone. c: nuclease activity on RNAs originating from Acanthamoeba castellanii ; no treatment (a); buffer (b); TupBlac in the absence (c, d) or presence (e) of sulbactam. d: nuclease activity on RNAs originating from bacteria that differ by the G+C-content of their genome, as indicated at the top of the digital gel image. For each RNA, three samples were analyzed: no treatment (1); treatment with TupBlac in the absence of sulbactam (2); and treatment with TupBlac in the presence of sulbactam (3).

    Journal: bioRxiv

    Article Title: A metallo-beta-lactamase with both beta-lactamase and ribonuclease activity is linked with traduction in giant viruses

    doi: 10.1101/819797

    Figure Lengend Snippet: Digital gel images of RNase activity of expressed Tupanvirus protein TupBlac on E. coli RNA. RNA samples (1 μg) were incubated with 15 μg of TupBlac at 30°C in the absence or presence of 10 μg/mL of sulbactam or 200 μM of ceftriaxone. Nuclease activity was visualized as digital gel images performed using the Agilent Bioanalyzer 2100 with the RNA 6000 Pico LabChip (Agilent Technologies, Palo Alto, CA). a: RNA used as substrate was from Escherichia coli; no treatment (a); buffer (b); sulbactam (c); TupBlac in the absence (d) or presence (e) of sulbactam. b: RNA used as substrate was from Escherichia coli; reactions were stopped at different times (5 min, 10 min, 30 min, 1 h and 2 h) by the addition of proteinase K (10 μg) and incubation for 1h at 37°C. The first lane corresponds to no treatment; lanes 2 to 6 to RNA treatment with TupBlac in the absence of ceftriaxone; lanes 7 to 11 to RNA treatment with TupBlac in the presence of ceftriaxone. c: nuclease activity on RNAs originating from Acanthamoeba castellanii ; no treatment (a); buffer (b); TupBlac in the absence (c, d) or presence (e) of sulbactam. d: nuclease activity on RNAs originating from bacteria that differ by the G+C-content of their genome, as indicated at the top of the digital gel image. For each RNA, three samples were analyzed: no treatment (1); treatment with TupBlac in the absence of sulbactam (2); and treatment with TupBlac in the presence of sulbactam (3).

    Article Snippet: Enzymatic reactions were performed by incubating each polynucleotide (2 μg) with 15 μg of the expressed Tupanvirus protein TupBlac in Tris-HCl buffer 50 mM, pH 8.0, sodium chloride 0.3 M, using a final volume of 20 μL at 30°C for 2 h. After incubation, the material was loaded onto denaturing polyacrylamide gel electrophoresis (dPAGE) at 12% or analysed using the Agilent RNA 6000 Pico LabChip kit on an Agilent 2100 Bioanalyzer (Agilent Technology, Palo Alto, CA, USA).

    Techniques: Activity Assay, Incubation