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Agilent technologies rna 6000 pico labchip kit
Integrity of the RNA samples. Each bar depicts the arithmetic mean of the RNA integrity numbers (RIN) measured for the 10 whole RNA samples, which were used for the microarray hybridisation per cell population and per time point. The error bars indicate standard deviations. This quality control was carried out using the Agilent Bioanalyzer 2100 and the <t>RNA</t> 6000 Pico <t>LabChip</t> kit (Agilent Technologies, Waldbronn, Germany).
Rna 6000 Pico Labchip Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rna 6000 pico labchip kit/product/Agilent technologies
Average 99 stars, based on 18 article reviews
Price from $9.99 to $1999.99
rna 6000 pico labchip kit - by Bioz Stars, 2020-01
99/100 stars

Images

1) Product Images from "Microarray data of transcriptome shifts in blood cell subsets during S1P receptor modulator therapy"

Article Title: Microarray data of transcriptome shifts in blood cell subsets during S1P receptor modulator therapy

Journal: Scientific Data

doi: 10.1038/sdata.2018.145

Integrity of the RNA samples. Each bar depicts the arithmetic mean of the RNA integrity numbers (RIN) measured for the 10 whole RNA samples, which were used for the microarray hybridisation per cell population and per time point. The error bars indicate standard deviations. This quality control was carried out using the Agilent Bioanalyzer 2100 and the RNA 6000 Pico LabChip kit (Agilent Technologies, Waldbronn, Germany).
Figure Legend Snippet: Integrity of the RNA samples. Each bar depicts the arithmetic mean of the RNA integrity numbers (RIN) measured for the 10 whole RNA samples, which were used for the microarray hybridisation per cell population and per time point. The error bars indicate standard deviations. This quality control was carried out using the Agilent Bioanalyzer 2100 and the RNA 6000 Pico LabChip kit (Agilent Technologies, Waldbronn, Germany).

Techniques Used: Microarray, Hybridization

2) Product Images from "Microarray data of transcriptome shifts in blood cell subsets during S1P receptor modulator therapy"

Article Title: Microarray data of transcriptome shifts in blood cell subsets during S1P receptor modulator therapy

Journal: Scientific Data

doi: 10.1038/sdata.2018.145

Integrity of the RNA samples. Each bar depicts the arithmetic mean of the RNA integrity numbers (RIN) measured for the 10 whole RNA samples, which were used for the microarray hybridisation per cell population and per time point. The error bars indicate standard deviations. This quality control was carried out using the Agilent Bioanalyzer 2100 and the RNA 6000 Pico LabChip kit (Agilent Technologies, Waldbronn, Germany).
Figure Legend Snippet: Integrity of the RNA samples. Each bar depicts the arithmetic mean of the RNA integrity numbers (RIN) measured for the 10 whole RNA samples, which were used for the microarray hybridisation per cell population and per time point. The error bars indicate standard deviations. This quality control was carried out using the Agilent Bioanalyzer 2100 and the RNA 6000 Pico LabChip kit (Agilent Technologies, Waldbronn, Germany).

Techniques Used: Microarray, Hybridization

3) Product Images from "Maintaining RNA integrity in a homogeneous population of mammary epithelial cells isolated by Laser Capture Microdissection"

Article Title: Maintaining RNA integrity in a homogeneous population of mammary epithelial cells isolated by Laser Capture Microdissection

Journal: BMC Cell Biology

doi: 10.1186/1471-2121-11-95

Impact of the freezing system on morphology of fresh mammary tissue sections and on quality of RNA extracted . Alveolar (acini) structures lined by MECs (yellow arrows) can be easily distinguished after staining mammary tissue sections with Cresyl violet AMBION. Immediately after collection, mammary tissue samples were washed in cold PBS solution, cut in cube of 3-4 mm thickness, and frozen in four different conditions: pieces of mammary tissue were either directly introduced into 1.5-ml eppendorf tubes and frozen in liquid nitrogen (A), or in cold isopentane (-80°C) using the SnapFrost™ system (C), or embedded in OCT ® contained in cryomold before to be immediately immerged in liquid nitrogen (B) or in cold isopentane (D). RNA quality (RIN) which was estimated by RNA 6000 Pico LabChip kit and Agilent 2100 Bioanalyzer, was identical (ranging between 8.5 and 9.5) whatever the freezing procedure, as illustrated in the electrophoreris profiles. Some large blisters (red arrows on Figure A and B) appear however in biopsy flash frozen in liquid nitrogen, mainly without cryoprotector, and morphological details are better seen on tissues frozen using the SnapFrost™ system (Magnification: ×60). The green arrow indicates the thermoplastic film stuck on epithelial cells to be captured.
Figure Legend Snippet: Impact of the freezing system on morphology of fresh mammary tissue sections and on quality of RNA extracted . Alveolar (acini) structures lined by MECs (yellow arrows) can be easily distinguished after staining mammary tissue sections with Cresyl violet AMBION. Immediately after collection, mammary tissue samples were washed in cold PBS solution, cut in cube of 3-4 mm thickness, and frozen in four different conditions: pieces of mammary tissue were either directly introduced into 1.5-ml eppendorf tubes and frozen in liquid nitrogen (A), or in cold isopentane (-80°C) using the SnapFrost™ system (C), or embedded in OCT ® contained in cryomold before to be immediately immerged in liquid nitrogen (B) or in cold isopentane (D). RNA quality (RIN) which was estimated by RNA 6000 Pico LabChip kit and Agilent 2100 Bioanalyzer, was identical (ranging between 8.5 and 9.5) whatever the freezing procedure, as illustrated in the electrophoreris profiles. Some large blisters (red arrows on Figure A and B) appear however in biopsy flash frozen in liquid nitrogen, mainly without cryoprotector, and morphological details are better seen on tissues frozen using the SnapFrost™ system (Magnification: ×60). The green arrow indicates the thermoplastic film stuck on epithelial cells to be captured.

Techniques Used: Staining

4) Product Images from "Enhanced molecular analyses by combination of the HOPE-technique and laser microdissection"

Article Title: Enhanced molecular analyses by combination of the HOPE-technique and laser microdissection

Journal: Diagnostic Pathology

doi: 10.1186/1746-1596-1-2

Results of RNA-integrity-testing by a Agilent Bioanalyzer 2100 using the RNA 6000 Pico LabChip kit. Lane 1 and 2 are HOPE-fixed materials and lane 3 is FFPE.
Figure Legend Snippet: Results of RNA-integrity-testing by a Agilent Bioanalyzer 2100 using the RNA 6000 Pico LabChip kit. Lane 1 and 2 are HOPE-fixed materials and lane 3 is FFPE.

Techniques Used: Formalin-fixed Paraffin-Embedded

Related Articles

DNA Extraction:

Article Title: Characterization of bovine embryos cultured under conditions appropriate for sustaining human naïve pluripotency
Article Snippet: Paragraph title: ICM collection and RNA or DNA isolation ... A Bioanalyzer 2100 and the RNA 6000 Pico LabChip kit (Agilent Technologies, Amstelveen, The Netherlands) were used for assessment of total RNA quality and quantity per manufacturer’s instructions.

RNA Extraction:

Article Title: Enhanced molecular analyses by combination of the HOPE-technique and laser microdissection
Article Snippet: Paragraph title: RNA extraction ... Of each RNA sample 1 μl was tested in an Agilent Bioanalyzer 2100 using the RNA 6000 Pico LabChip kit (Agilent, Waldbronn, Germany, #5065-4473) to determine the RNA integrity.

Formalin-fixed Paraffin-Embedded:

Article Title: miR-29c is downregulated in renal interstitial fibrosis in humans and rats and restored by HIF-? activation
Article Snippet: Total RNA from formalin-fixed and paraffin-embedded (FFPE) tissue was extracted by using a RecoverALL total nucleic acid isolation kit (Ambion, Austin, TX) according to the manufacturer's protocol. .. The quality check of RNA was performed using a 2100 Bioanalyzer with an RNA 6000 Pico LabChip kit (Agilent Technologies, Santa Clara, CA).

Concentration Assay:

Article Title: miR-29c is downregulated in renal interstitial fibrosis in humans and rats and restored by HIF-? activation
Article Snippet: The concentration was quantified by a NanoDrop 1000 Spectrophotometer (NanoDrop Technologies, Waltham, MA). .. The quality check of RNA was performed using a 2100 Bioanalyzer with an RNA 6000 Pico LabChip kit (Agilent Technologies, Santa Clara, CA).

Article Title: Enhanced molecular analyses by combination of the HOPE-technique and laser microdissection
Article Snippet: Spectrophotometry was performed for testing concentration and purity of RNA, which revealed well-preserved RNA of high concentration throughout the samples. .. Of each RNA sample 1 μl was tested in an Agilent Bioanalyzer 2100 using the RNA 6000 Pico LabChip kit (Agilent, Waldbronn, Germany, #5065-4473) to determine the RNA integrity.

Article Title: Maintaining RNA integrity in a homogeneous population of mammary epithelial cells isolated by Laser Capture Microdissection
Article Snippet: .. The fluorimetric method and micro-capillary electrophoresis device developed by Agilent Technologies was chosen to determine RNA concentration and quality with RNA 6000 pico LabChip Kit in the Agilent Bioanalyzer 2100 system. .. Quality was evaluated using the RNA Integrity Number (RIN) value introduced by Agilent [ ].

Isolation:

Article Title: MicroRNA-221-3p, a TWIST2 target, promotes cervical cancer metastasis by directly targeting THBS2
Article Snippet: In brief, total RNA was extracted from cells or tissues samples using the miRVana miRNA Isolation Kit (Ambion Inc., TX, USA). .. The quality and quantity of RNA samples were assessed by a 2100 Bioanalyzer using the RNA 6000 Pico LabChip kit (Agilent Technologies, Santa Clara, CA).

Article Title: miR-29c is downregulated in renal interstitial fibrosis in humans and rats and restored by HIF-? activation
Article Snippet: Paragraph title: RNA isolation. ... The quality check of RNA was performed using a 2100 Bioanalyzer with an RNA 6000 Pico LabChip kit (Agilent Technologies, Santa Clara, CA).

Article Title: Characterization of bovine embryos cultured under conditions appropriate for sustaining human naïve pluripotency
Article Snippet: RNA isolation and on column DNA digestion was performed using an RNA micro kit (Qiagen) according to manufacturer’s protocol. .. A Bioanalyzer 2100 and the RNA 6000 Pico LabChip kit (Agilent Technologies, Amstelveen, The Netherlands) were used for assessment of total RNA quality and quantity per manufacturer’s instructions.

Article Title: Expression of pro-apoptotic markers is increased along the osteochondral junction in naturally occurring osteochondrosis
Article Snippet: Paragraph title: 2.4. RNA isolation ... RNA quality control was performed using an Agilent 2100 Bioanalyzer and the RNA 6000 Pico LabChip kit (Agilent Technologies, Santa Clara, CA, USA).

Article Title: Maintaining RNA integrity in a homogeneous population of mammary epithelial cells isolated by Laser Capture Microdissection
Article Snippet: RNA quality control and cDNA synthesis Purity, concentration and integrity of total RNA isolated in this manner were assessed using two independent techniques. .. The fluorimetric method and micro-capillary electrophoresis device developed by Agilent Technologies was chosen to determine RNA concentration and quality with RNA 6000 pico LabChip Kit in the Agilent Bioanalyzer 2100 system.

Article Title: Up-Regulation of MicroRNA-145 Associates with Lymph Node Metastasis in Colorectal Cancer
Article Snippet: Briefly, total RNA was extracted from tumor samples using the miRVana miRNA Isolation Kit (Ambion Inc., TX, USA). .. The quality and quantity of RNA samples were assessed by a 2100 Bioanalyzer using the RNA 6000 Pico LabChip kit (Agilent Technologies, Santa Clara, CA).

Article Title: A mRNA landscape of bovine embryos after standard and MAPK-inhibited culture conditions: a comparative analysis
Article Snippet: Paragraph title: RNA isolation ... Total RNA quality and quantity assessment was performed by micro-electrophoresis on a Bioanalyzer 2100 using the RNA 6000 Pico LabChip kit (Agilent Technologies, Amstelveen, The Netherlands) according to manufacturer’s instructions.

Article Title: Genome-Wide Screening of Genes Regulated by DNA Methylation in Colon Cancer Development
Article Snippet: .. The quality of the isolated nucleic acid was measured by microcapillary electrophoresis system using RNA 6000 Pico LabChip kit (Agilent BioAnalyzer 2100). .. For the microarray analysis 5–50 ng total RNA was used from the samples with RIN (RNA Integrity Number) between 7–10.

Article Title: The Expression of miR-375 Is Associated with Carcinogenesis in Three Subtypes of Lung Cancer
Article Snippet: Paragraph title: RNA isolation ... The quality control of RNA was performed by a 2100 Bioanalyzer using the RNA 6000 Pico LabChip kit (Agilent Technologies, Santa Clara, CA).

Laser Capture Microdissection:

Article Title: Expression of pro-apoptotic markers is increased along the osteochondral junction in naturally occurring osteochondrosis
Article Snippet: PicoPure RNA Isolation Kit (Arcturus Bioscience, Mountainview, CA, USA) was used for RNA isolation of LCM samples with slight modifications. .. RNA quality control was performed using an Agilent 2100 Bioanalyzer and the RNA 6000 Pico LabChip kit (Agilent Technologies, Santa Clara, CA, USA).

Generated:

Article Title: miR-29c is downregulated in renal interstitial fibrosis in humans and rats and restored by HIF-? activation
Article Snippet: The quality check of RNA was performed using a 2100 Bioanalyzer with an RNA 6000 Pico LabChip kit (Agilent Technologies, Santa Clara, CA). .. An RNA integrity number (RIN) score was generated for each sample on a scale of 1–10 as an indication of RNA quality ( , , , ).

Spectrophotometry:

Article Title: miR-29c is downregulated in renal interstitial fibrosis in humans and rats and restored by HIF-? activation
Article Snippet: The concentration was quantified by a NanoDrop 1000 Spectrophotometer (NanoDrop Technologies, Waltham, MA). .. The quality check of RNA was performed using a 2100 Bioanalyzer with an RNA 6000 Pico LabChip kit (Agilent Technologies, Santa Clara, CA).

Article Title: Enhanced molecular analyses by combination of the HOPE-technique and laser microdissection
Article Snippet: Spectrophotometry was performed for testing concentration and purity of RNA, which revealed well-preserved RNA of high concentration throughout the samples. .. Of each RNA sample 1 μl was tested in an Agilent Bioanalyzer 2100 using the RNA 6000 Pico LabChip kit (Agilent, Waldbronn, Germany, #5065-4473) to determine the RNA integrity.

Article Title: Maintaining RNA integrity in a homogeneous population of mammary epithelial cells isolated by Laser Capture Microdissection
Article Snippet: RNA purity was evaluated by absorbance readings (Ratio A260/A230 and A260/A280) using the NanoDrop ND-1000 spectrophotometer (Thermo Fisher Scientific, Wilmington, DE). .. The fluorimetric method and micro-capillary electrophoresis device developed by Agilent Technologies was chosen to determine RNA concentration and quality with RNA 6000 pico LabChip Kit in the Agilent Bioanalyzer 2100 system.

Article Title: Gene Expression Profiling of Endoplasmic Reticulum Stress in Hepatitis C Virus-Containing Cells Treated with an Inhibitor of Protein Disulfide Isomerases
Article Snippet: .. The RNA was quantified using the NanoDrop ND-1000 spectrophotometer, and the quality of the RNA was verified using the RNA 6000 Pico LabChip kit (Agilent Technologies, Mississauga, Ontario). .. Gene Expression Analysis Cellular RNA samples were hybridized to high-density oligonucleotide arrays (Affymetrix GeneChip HG-U133 Plus2).

Purification:

Article Title: Enhanced molecular analyses by combination of the HOPE-technique and laser microdissection
Article Snippet: Briefly, the cells were dissolved in 350 μl of RLT lysis buffer, treated with DNaseI after the first washing step according to the manual and finally the purified RNA was eluted in 12 μl of RNase free water. .. Of each RNA sample 1 μl was tested in an Agilent Bioanalyzer 2100 using the RNA 6000 Pico LabChip kit (Agilent, Waldbronn, Germany, #5065-4473) to determine the RNA integrity.

Electrophoresis:

Article Title: Maintaining RNA integrity in a homogeneous population of mammary epithelial cells isolated by Laser Capture Microdissection
Article Snippet: .. The fluorimetric method and micro-capillary electrophoresis device developed by Agilent Technologies was chosen to determine RNA concentration and quality with RNA 6000 pico LabChip Kit in the Agilent Bioanalyzer 2100 system. .. Quality was evaluated using the RNA Integrity Number (RIN) value introduced by Agilent [ ].

Article Title: Genome-Wide Screening of Genes Regulated by DNA Methylation in Colon Cancer Development
Article Snippet: .. The quality of the isolated nucleic acid was measured by microcapillary electrophoresis system using RNA 6000 Pico LabChip kit (Agilent BioAnalyzer 2100). .. For the microarray analysis 5–50 ng total RNA was used from the samples with RIN (RNA Integrity Number) between 7–10.

Microarray:

Article Title: MicroRNA-221-3p, a TWIST2 target, promotes cervical cancer metastasis by directly targeting THBS2
Article Snippet: Paragraph title: miRNAs microarray ... The quality and quantity of RNA samples were assessed by a 2100 Bioanalyzer using the RNA 6000 Pico LabChip kit (Agilent Technologies, Santa Clara, CA).

Article Title: miR-29c is downregulated in renal interstitial fibrosis in humans and rats and restored by HIF-? activation
Article Snippet: For microRNA microarray experiments, total RNA was extracted from the tissue samples using a mirVana miRNA isolation kit (Applied Biosystems, Foster City, CA). .. The quality check of RNA was performed using a 2100 Bioanalyzer with an RNA 6000 Pico LabChip kit (Agilent Technologies, Santa Clara, CA).

Article Title: Up-Regulation of MicroRNA-145 Associates with Lymph Node Metastasis in Colorectal Cancer
Article Snippet: Paragraph title: Microarray analysis ... The quality and quantity of RNA samples were assessed by a 2100 Bioanalyzer using the RNA 6000 Pico LabChip kit (Agilent Technologies, Santa Clara, CA).

Article Title: MicroRNA-17-5p promotes chemotherapeutic drug resistance and tumour metastasis of colorectal cancer by repressing PTEN expression
Article Snippet: Paragraph title: Microarray ... The quality control of RNA was performed by a 2100 Bioanalyzer using the RNA 6000 Pico LabChip kit (Agilent Technologies, Santa Clara, CA).

Article Title: Microarray data of transcriptome shifts in blood cell subsets during S1P receptor modulator therapy
Article Snippet: The RNA quality was controlled using standardised operating procedures (SOPs) at the Core Facility for Microarray Analysis of the University of Rostock. .. The integrity of the RNA samples was checked using an Agilent Bioanalyzer 2100 with the RNA 6000 Pico LabChip kit (both from Agilent Technologies, Waldbronn, Germany).

Article Title: Genome-Wide Screening of Genes Regulated by DNA Methylation in Colon Cancer Development
Article Snippet: The quality of the isolated nucleic acid was measured by microcapillary electrophoresis system using RNA 6000 Pico LabChip kit (Agilent BioAnalyzer 2100). .. For the microarray analysis 5–50 ng total RNA was used from the samples with RIN (RNA Integrity Number) between 7–10.

Article Title: Gene Expression Profiling of Endoplasmic Reticulum Stress in Hepatitis C Virus-Containing Cells Treated with an Inhibitor of Protein Disulfide Isomerases
Article Snippet: Paragraph title: Total RNA Preparation for Microarray Experiments ... The RNA was quantified using the NanoDrop ND-1000 spectrophotometer, and the quality of the RNA was verified using the RNA 6000 Pico LabChip kit (Agilent Technologies, Mississauga, Ontario).

Incubation:

Article Title: Maintaining RNA integrity in a homogeneous population of mammary epithelial cells isolated by Laser Capture Microdissection
Article Snippet: The fluorimetric method and micro-capillary electrophoresis device developed by Agilent Technologies was chosen to determine RNA concentration and quality with RNA 6000 pico LabChip Kit in the Agilent Bioanalyzer 2100 system. .. Then, 1 μl of RNase H (2 U/μl, Invitrogen) was added and incubated 20 min at 37°C to remove RNA.

Inhibition:

Article Title: Microarray data of transcriptome shifts in blood cell subsets during S1P receptor modulator therapy
Article Snippet: The integrity of the RNA samples was checked using an Agilent Bioanalyzer 2100 with the RNA 6000 Pico LabChip kit (both from Agilent Technologies, Waldbronn, Germany). .. One major effect of the treatment with fingolimod is the inhibition of lymphocyte egress from lymphoid tissues towards the peripheral blood .

Microscopy:

Article Title: Characterization of bovine embryos cultured under conditions appropriate for sustaining human naïve pluripotency
Article Snippet: In short, blastocysts were placed in wash buffer and sharpened tungsten needles were used to separate the ICM from the TE under a stereo microscope. .. A Bioanalyzer 2100 and the RNA 6000 Pico LabChip kit (Agilent Technologies, Amstelveen, The Netherlands) were used for assessment of total RNA quality and quantity per manufacturer’s instructions.

Expressing:

Article Title: MicroRNA-221-3p, a TWIST2 target, promotes cervical cancer metastasis by directly targeting THBS2
Article Snippet: miRNAs microarray Tissues (six CC tissues derived from stage I and II, respectively) and cells (SiHa, SiHa-shtw2, SiHa-tw2 cells) were collected and the expression profiles of miRNAs were determined using Agilent miRNA microarray 21.0. .. The quality and quantity of RNA samples were assessed by a 2100 Bioanalyzer using the RNA 6000 Pico LabChip kit (Agilent Technologies, Santa Clara, CA).

Article Title: Up-Regulation of MicroRNA-145 Associates with Lymph Node Metastasis in Colorectal Cancer
Article Snippet: Microarray analysis Eight primary colorectal cancer tissues derived from stage II–III colorectal cancer patients with (n = 4) or without (n = 4) lymph node metastasis were collected and the expression profiles of miRNA were determined using Agilent miRNA microarray. .. The quality and quantity of RNA samples were assessed by a 2100 Bioanalyzer using the RNA 6000 Pico LabChip kit (Agilent Technologies, Santa Clara, CA).

Lysis:

Article Title: Enhanced molecular analyses by combination of the HOPE-technique and laser microdissection
Article Snippet: Briefly, the cells were dissolved in 350 μl of RLT lysis buffer, treated with DNaseI after the first washing step according to the manual and finally the purified RNA was eluted in 12 μl of RNase free water. .. Of each RNA sample 1 μl was tested in an Agilent Bioanalyzer 2100 using the RNA 6000 Pico LabChip kit (Agilent, Waldbronn, Germany, #5065-4473) to determine the RNA integrity.

Software:

Article Title: MicroRNA-221-3p, a TWIST2 target, promotes cervical cancer metastasis by directly targeting THBS2
Article Snippet: The quality and quantity of RNA samples were assessed by a 2100 Bioanalyzer using the RNA 6000 Pico LabChip kit (Agilent Technologies, Santa Clara, CA). .. Data analysis was performed using GeneSpringGX software 11.0 (Agilent Technologies).

Labeling:

Article Title: Up-Regulation of MicroRNA-145 Associates with Lymph Node Metastasis in Colorectal Cancer
Article Snippet: The quality and quantity of RNA samples were assessed by a 2100 Bioanalyzer using the RNA 6000 Pico LabChip kit (Agilent Technologies, Santa Clara, CA). .. The microarray experiments were performed at ShanghaiBio Corporation using Agilent miRNA labeling reagent and Hybridization Kits, Agilent human miRNA array (V2) and Agilent microarray scanner.

Synthesized:

Article Title: Maintaining RNA integrity in a homogeneous population of mammary epithelial cells isolated by Laser Capture Microdissection
Article Snippet: The fluorimetric method and micro-capillary electrophoresis device developed by Agilent Technologies was chosen to determine RNA concentration and quality with RNA 6000 pico LabChip Kit in the Agilent Bioanalyzer 2100 system. .. First-strand cDNA was synthesized from 5-10 ng total RNA primed with oligo(dT)20 and random primers (3:1, v/v) using Superscript III reverse transcriptase (Invitrogen, Applied Biosystems® ) according to manufacturer's instructions.

Derivative Assay:

Article Title: MicroRNA-221-3p, a TWIST2 target, promotes cervical cancer metastasis by directly targeting THBS2
Article Snippet: miRNAs microarray Tissues (six CC tissues derived from stage I and II, respectively) and cells (SiHa, SiHa-shtw2, SiHa-tw2 cells) were collected and the expression profiles of miRNAs were determined using Agilent miRNA microarray 21.0. .. The quality and quantity of RNA samples were assessed by a 2100 Bioanalyzer using the RNA 6000 Pico LabChip kit (Agilent Technologies, Santa Clara, CA).

Article Title: Up-Regulation of MicroRNA-145 Associates with Lymph Node Metastasis in Colorectal Cancer
Article Snippet: Microarray analysis Eight primary colorectal cancer tissues derived from stage II–III colorectal cancer patients with (n = 4) or without (n = 4) lymph node metastasis were collected and the expression profiles of miRNA were determined using Agilent miRNA microarray. .. The quality and quantity of RNA samples were assessed by a 2100 Bioanalyzer using the RNA 6000 Pico LabChip kit (Agilent Technologies, Santa Clara, CA).

Article Title: MicroRNA-17-5p promotes chemotherapeutic drug resistance and tumour metastasis of colorectal cancer by repressing PTEN expression
Article Snippet: The quality control of RNA was performed by a 2100 Bioanalyzer using the RNA 6000 Pico LabChip kit (Agilent Technologies, Santa Clara, CA). .. Total RNA (100 ng) derived from each of the specimens were used as inputs for labelling via Cy3 incorporation.

Hybridization:

Article Title: Up-Regulation of MicroRNA-145 Associates with Lymph Node Metastasis in Colorectal Cancer
Article Snippet: The quality and quantity of RNA samples were assessed by a 2100 Bioanalyzer using the RNA 6000 Pico LabChip kit (Agilent Technologies, Santa Clara, CA). .. The microarray experiments were performed at ShanghaiBio Corporation using Agilent miRNA labeling reagent and Hybridization Kits, Agilent human miRNA array (V2) and Agilent microarray scanner.

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    Agilent technologies rna 6000 pico labchip kit
    Integrity of the RNA samples. Each bar depicts the arithmetic mean of the RNA integrity numbers (RIN) measured for the 10 whole RNA samples, which were used for the microarray hybridisation per cell population and per time point. The error bars indicate standard deviations. This quality control was carried out using the Agilent Bioanalyzer 2100 and the <t>RNA</t> 6000 Pico <t>LabChip</t> kit (Agilent Technologies, Waldbronn, Germany).
    Rna 6000 Pico Labchip Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rna 6000 pico labchip kit/product/Agilent technologies
    Average 99 stars, based on 18 article reviews
    Price from $9.99 to $1999.99
    rna 6000 pico labchip kit - by Bioz Stars, 2020-01
    99/100 stars
      Buy from Supplier

    Image Search Results


    Integrity of the RNA samples. Each bar depicts the arithmetic mean of the RNA integrity numbers (RIN) measured for the 10 whole RNA samples, which were used for the microarray hybridisation per cell population and per time point. The error bars indicate standard deviations. This quality control was carried out using the Agilent Bioanalyzer 2100 and the RNA 6000 Pico LabChip kit (Agilent Technologies, Waldbronn, Germany).

    Journal: Scientific Data

    Article Title: Microarray data of transcriptome shifts in blood cell subsets during S1P receptor modulator therapy

    doi: 10.1038/sdata.2018.145

    Figure Lengend Snippet: Integrity of the RNA samples. Each bar depicts the arithmetic mean of the RNA integrity numbers (RIN) measured for the 10 whole RNA samples, which were used for the microarray hybridisation per cell population and per time point. The error bars indicate standard deviations. This quality control was carried out using the Agilent Bioanalyzer 2100 and the RNA 6000 Pico LabChip kit (Agilent Technologies, Waldbronn, Germany).

    Article Snippet: The integrity of the RNA samples was checked using an Agilent Bioanalyzer 2100 with the RNA 6000 Pico LabChip kit (both from Agilent Technologies, Waldbronn, Germany).

    Techniques: Microarray, Hybridization

    Integrity of the RNA samples. Each bar depicts the arithmetic mean of the RNA integrity numbers (RIN) measured for the 10 whole RNA samples, which were used for the microarray hybridisation per cell population and per time point. The error bars indicate standard deviations. This quality control was carried out using the Agilent Bioanalyzer 2100 and the RNA 6000 Pico LabChip kit (Agilent Technologies, Waldbronn, Germany).

    Journal: Scientific Data

    Article Title: Microarray data of transcriptome shifts in blood cell subsets during S1P receptor modulator therapy

    doi: 10.1038/sdata.2018.145

    Figure Lengend Snippet: Integrity of the RNA samples. Each bar depicts the arithmetic mean of the RNA integrity numbers (RIN) measured for the 10 whole RNA samples, which were used for the microarray hybridisation per cell population and per time point. The error bars indicate standard deviations. This quality control was carried out using the Agilent Bioanalyzer 2100 and the RNA 6000 Pico LabChip kit (Agilent Technologies, Waldbronn, Germany).

    Article Snippet: The integrity of the RNA samples was checked using an Agilent Bioanalyzer 2100 with the RNA 6000 Pico LabChip kit (both from Agilent Technologies, Waldbronn, Germany).

    Techniques: Microarray, Hybridization

    Impact of the freezing system on morphology of fresh mammary tissue sections and on quality of RNA extracted . Alveolar (acini) structures lined by MECs (yellow arrows) can be easily distinguished after staining mammary tissue sections with Cresyl violet AMBION. Immediately after collection, mammary tissue samples were washed in cold PBS solution, cut in cube of 3-4 mm thickness, and frozen in four different conditions: pieces of mammary tissue were either directly introduced into 1.5-ml eppendorf tubes and frozen in liquid nitrogen (A), or in cold isopentane (-80°C) using the SnapFrost™ system (C), or embedded in OCT ® contained in cryomold before to be immediately immerged in liquid nitrogen (B) or in cold isopentane (D). RNA quality (RIN) which was estimated by RNA 6000 Pico LabChip kit and Agilent 2100 Bioanalyzer, was identical (ranging between 8.5 and 9.5) whatever the freezing procedure, as illustrated in the electrophoreris profiles. Some large blisters (red arrows on Figure A and B) appear however in biopsy flash frozen in liquid nitrogen, mainly without cryoprotector, and morphological details are better seen on tissues frozen using the SnapFrost™ system (Magnification: ×60). The green arrow indicates the thermoplastic film stuck on epithelial cells to be captured.

    Journal: BMC Cell Biology

    Article Title: Maintaining RNA integrity in a homogeneous population of mammary epithelial cells isolated by Laser Capture Microdissection

    doi: 10.1186/1471-2121-11-95

    Figure Lengend Snippet: Impact of the freezing system on morphology of fresh mammary tissue sections and on quality of RNA extracted . Alveolar (acini) structures lined by MECs (yellow arrows) can be easily distinguished after staining mammary tissue sections with Cresyl violet AMBION. Immediately after collection, mammary tissue samples were washed in cold PBS solution, cut in cube of 3-4 mm thickness, and frozen in four different conditions: pieces of mammary tissue were either directly introduced into 1.5-ml eppendorf tubes and frozen in liquid nitrogen (A), or in cold isopentane (-80°C) using the SnapFrost™ system (C), or embedded in OCT ® contained in cryomold before to be immediately immerged in liquid nitrogen (B) or in cold isopentane (D). RNA quality (RIN) which was estimated by RNA 6000 Pico LabChip kit and Agilent 2100 Bioanalyzer, was identical (ranging between 8.5 and 9.5) whatever the freezing procedure, as illustrated in the electrophoreris profiles. Some large blisters (red arrows on Figure A and B) appear however in biopsy flash frozen in liquid nitrogen, mainly without cryoprotector, and morphological details are better seen on tissues frozen using the SnapFrost™ system (Magnification: ×60). The green arrow indicates the thermoplastic film stuck on epithelial cells to be captured.

    Article Snippet: The fluorimetric method and micro-capillary electrophoresis device developed by Agilent Technologies was chosen to determine RNA concentration and quality with RNA 6000 pico LabChip Kit in the Agilent Bioanalyzer 2100 system.

    Techniques: Staining

    Results of RNA-integrity-testing by a Agilent Bioanalyzer 2100 using the RNA 6000 Pico LabChip kit. Lane 1 and 2 are HOPE-fixed materials and lane 3 is FFPE.

    Journal: Diagnostic Pathology

    Article Title: Enhanced molecular analyses by combination of the HOPE-technique and laser microdissection

    doi: 10.1186/1746-1596-1-2

    Figure Lengend Snippet: Results of RNA-integrity-testing by a Agilent Bioanalyzer 2100 using the RNA 6000 Pico LabChip kit. Lane 1 and 2 are HOPE-fixed materials and lane 3 is FFPE.

    Article Snippet: Of each RNA sample 1 μl was tested in an Agilent Bioanalyzer 2100 using the RNA 6000 Pico LabChip kit (Agilent, Waldbronn, Germany, #5065-4473) to determine the RNA integrity.

    Techniques: Formalin-fixed Paraffin-Embedded