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Agilent technologies rna 6000 pico kit
Characterization of exosomal RNA (esRNA) extracted from purified nanovesicles from fresh human aqueous humor. The esRNA was evaluated using Agilent Bioanalyzer 2100 with Agilent <t>RNA</t> 6000 Pico Kit. A) Shows the RNA ladder and B) displays extracted esRNA from one of the human aqueous humor samples. The size of extracted esRNA was less than 200 nucleotides (nt), mostly around 25 nt. C) shows the miRNAs that were identified via small RNA sequencing using Illumina MiSeq sequencing system. The height of each bar indicated the number of sequence reads with perfect match to mature miRNA.
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1) Product Images from "Human Aqueous Humor Exosomes"

Article Title: Human Aqueous Humor Exosomes

Journal: Experimental eye research

doi: 10.1016/j.exer.2015.01.019

Characterization of exosomal RNA (esRNA) extracted from purified nanovesicles from fresh human aqueous humor. The esRNA was evaluated using Agilent Bioanalyzer 2100 with Agilent RNA 6000 Pico Kit. A) Shows the RNA ladder and B) displays extracted esRNA from one of the human aqueous humor samples. The size of extracted esRNA was less than 200 nucleotides (nt), mostly around 25 nt. C) shows the miRNAs that were identified via small RNA sequencing using Illumina MiSeq sequencing system. The height of each bar indicated the number of sequence reads with perfect match to mature miRNA.
Figure Legend Snippet: Characterization of exosomal RNA (esRNA) extracted from purified nanovesicles from fresh human aqueous humor. The esRNA was evaluated using Agilent Bioanalyzer 2100 with Agilent RNA 6000 Pico Kit. A) Shows the RNA ladder and B) displays extracted esRNA from one of the human aqueous humor samples. The size of extracted esRNA was less than 200 nucleotides (nt), mostly around 25 nt. C) shows the miRNAs that were identified via small RNA sequencing using Illumina MiSeq sequencing system. The height of each bar indicated the number of sequence reads with perfect match to mature miRNA.

Techniques Used: Purification, RNA Sequencing Assay, Sequencing

2) Product Images from "Exosomes from bulk and stem cells from human prostate cancer have a differential microRNA content that contributes cooperatively over local and pre-metastatic niche"

Article Title: Exosomes from bulk and stem cells from human prostate cancer have a differential microRNA content that contributes cooperatively over local and pre-metastatic niche

Journal: Oncotarget

doi: 10.18632/oncotarget.6540

RNA profiles from exosomes from bulk cells (left) and CSCs (right) exosomes obtained by Agilent 2100 Bioanalyzer The electropherograms shows the size in distribution of the nucleotides (nt) and fluorescence intensity (FU) of Total RNA by Agilent RNA 6000 Pico Kit (A) and small RNAs after enrichment by the Agilent Small RNA Kit (B) All the electropherograms correspond to the sample of the same patient. The inserts show a representative image from the cultures under study.
Figure Legend Snippet: RNA profiles from exosomes from bulk cells (left) and CSCs (right) exosomes obtained by Agilent 2100 Bioanalyzer The electropherograms shows the size in distribution of the nucleotides (nt) and fluorescence intensity (FU) of Total RNA by Agilent RNA 6000 Pico Kit (A) and small RNAs after enrichment by the Agilent Small RNA Kit (B) All the electropherograms correspond to the sample of the same patient. The inserts show a representative image from the cultures under study.

Techniques Used: Fluorescence

3) Product Images from "Comparison of membrane affinity-based method with size-exclusion chromatography for isolation of exosome-like vesicles from human plasma"

Article Title: Comparison of membrane affinity-based method with size-exclusion chromatography for isolation of exosome-like vesicles from human plasma

Journal: Journal of Translational Medicine

doi: 10.1186/s12967-017-1374-6

RNA analysis of EVs isolated by exoEasy kit and SEC qEV columns. a RNA concentration. b Representative bioanalyzer profiles of RNA isolated from the same donor and analyzed by RNA 6000 Pico Kit; the y-axis shows fluorescence units (FU) and the x-axis the nucleotide length (nt) of the RNA. Peaks at 25 nt is an internal standard. c Ratio of total amount of RNA (µg) to total amount of protein (µg). (mean ± SEM, n = 6; *p
Figure Legend Snippet: RNA analysis of EVs isolated by exoEasy kit and SEC qEV columns. a RNA concentration. b Representative bioanalyzer profiles of RNA isolated from the same donor and analyzed by RNA 6000 Pico Kit; the y-axis shows fluorescence units (FU) and the x-axis the nucleotide length (nt) of the RNA. Peaks at 25 nt is an internal standard. c Ratio of total amount of RNA (µg) to total amount of protein (µg). (mean ± SEM, n = 6; *p

Techniques Used: Isolation, Size-exclusion Chromatography, Concentration Assay, Fluorescence

4) Product Images from "Time-Dependent Expression Profiles of microRNAs and mRNAs in Rat Milk Whey"

Article Title: Time-Dependent Expression Profiles of microRNAs and mRNAs in Rat Milk Whey

Journal: PLoS ONE

doi: 10.1371/journal.pone.0088843

Bioanalyzer analysis of RNAs in rat whey and serum. (A, B) Day 2 whey; (C, D) Day 9 whey; (E, F) Day 16 whey; (G, H) Serum; (A, C, E, G) Analysis using the RNA 6000 Pico Kit; (B, D, F, H) Analysis using the Small RNA Kit. Whey RNA concentrations were very high (especially in day 2 whey), so diluted RNA results are shown in this figure. FU = fluorescence units.
Figure Legend Snippet: Bioanalyzer analysis of RNAs in rat whey and serum. (A, B) Day 2 whey; (C, D) Day 9 whey; (E, F) Day 16 whey; (G, H) Serum; (A, C, E, G) Analysis using the RNA 6000 Pico Kit; (B, D, F, H) Analysis using the Small RNA Kit. Whey RNA concentrations were very high (especially in day 2 whey), so diluted RNA results are shown in this figure. FU = fluorescence units.

Techniques Used: Fluorescence

5) Product Images from "Gene expression analysis on small numbers of invasive cells collected by chemotaxis from primary mammary tumors of the mouse"

Article Title: Gene expression analysis on small numbers of invasive cells collected by chemotaxis from primary mammary tumors of the mouse

Journal: BMC Biotechnology

doi: 10.1186/1472-6750-3-13

The RNA from 200 and 400 cells is of equal amount and quality of RNA purified by conventional methods. Figure 5A : Total RNA isolated from microneedle collected cells was checked using Agilent Bioanalyzer and RNA 6000 Pico kit. The size distribution and rRNA ratio (28S/18S = 2.7) indicates good intactness of RNA sample. Figure 5B : A standard curve was generated for the CT value of a known quantity of RNA from a specific cell number using the β-actin primers for real-time PCR. The RNA from 200 and 400 cells were amplified by the SMART PCR method and run identically by real-time PCR. The CT values for the amplified RNA fall on the curve showing that the appropriate amount of the house keeping gene is present in the amplified sample. The CT values for the amplified samples are designated by the red asterisks.
Figure Legend Snippet: The RNA from 200 and 400 cells is of equal amount and quality of RNA purified by conventional methods. Figure 5A : Total RNA isolated from microneedle collected cells was checked using Agilent Bioanalyzer and RNA 6000 Pico kit. The size distribution and rRNA ratio (28S/18S = 2.7) indicates good intactness of RNA sample. Figure 5B : A standard curve was generated for the CT value of a known quantity of RNA from a specific cell number using the β-actin primers for real-time PCR. The RNA from 200 and 400 cells were amplified by the SMART PCR method and run identically by real-time PCR. The CT values for the amplified RNA fall on the curve showing that the appropriate amount of the house keeping gene is present in the amplified sample. The CT values for the amplified samples are designated by the red asterisks.

Techniques Used: Purification, Isolation, Generated, Real-time Polymerase Chain Reaction, Amplification, Polymerase Chain Reaction

6) Product Images from "Comparison of isolation methods of exosomes and exosomal RNA from cell culture medium and serum"

Article Title: Comparison of isolation methods of exosomes and exosomal RNA from cell culture medium and serum

Journal: International Journal of Molecular Medicine

doi: 10.3892/ijmm.2017.3080

Bioanalyzer analysis of total exosomal RNA (ExoRNA) by Agilent RNA Pico chip. The experiment was repeated three times and the trend was the same, thus one of the results is shown. The RNA 6000 ladder standard (in the first lane) contains six RNA fragments ranging in size from 0.2 to 6 kb. Representative bands of cellular RNA (in the second lane) showed 5S (120 nt), 18S (1,900 nt) and 28S rRNA (4,700 nt). In bands of exoRNA from cell culture medium (CCM), almost all of the samples showed an obvious band in the small RNA area. Among the five combination methods, Route_4 and Route_5 (labeled by **) showed a narrow size distribution pattern of small RNA around 100 nt. Some longer RNA species, including 18S and 28S ribosomal RNA, were found in bands obtained using Route_1, Route_2 and Route_3 (labeled by *). For exoRNA from serum, Route_e (labeled by **) had the most obvious band in the position of small RNA, and was followed by Route_b, Route_c and Route_d (labeled by *). No visible bands could be found in samples from Route_a and Route_f.
Figure Legend Snippet: Bioanalyzer analysis of total exosomal RNA (ExoRNA) by Agilent RNA Pico chip. The experiment was repeated three times and the trend was the same, thus one of the results is shown. The RNA 6000 ladder standard (in the first lane) contains six RNA fragments ranging in size from 0.2 to 6 kb. Representative bands of cellular RNA (in the second lane) showed 5S (120 nt), 18S (1,900 nt) and 28S rRNA (4,700 nt). In bands of exoRNA from cell culture medium (CCM), almost all of the samples showed an obvious band in the small RNA area. Among the five combination methods, Route_4 and Route_5 (labeled by **) showed a narrow size distribution pattern of small RNA around 100 nt. Some longer RNA species, including 18S and 28S ribosomal RNA, were found in bands obtained using Route_1, Route_2 and Route_3 (labeled by *). For exoRNA from serum, Route_e (labeled by **) had the most obvious band in the position of small RNA, and was followed by Route_b, Route_c and Route_d (labeled by *). No visible bands could be found in samples from Route_a and Route_f.

Techniques Used: Chromatin Immunoprecipitation, Cell Culture, Labeling

7) Product Images from "Comparison of membrane affinity-based method with size-exclusion chromatography for isolation of exosome-like vesicles from human plasma"

Article Title: Comparison of membrane affinity-based method with size-exclusion chromatography for isolation of exosome-like vesicles from human plasma

Journal: Journal of Translational Medicine

doi: 10.1186/s12967-017-1374-6

RNA analysis of EVs isolated by exoEasy kit and SEC qEV columns. a RNA concentration. b Representative bioanalyzer profiles of RNA isolated from the same donor and analyzed by RNA 6000 Pico Kit; the y-axis shows fluorescence units (FU) and the x-axis the nucleotide length (nt) of the RNA. Peaks at 25 nt is an internal standard. c Ratio of total amount of RNA (µg) to total amount of protein (µg). (mean ± SEM, n = 6; *p
Figure Legend Snippet: RNA analysis of EVs isolated by exoEasy kit and SEC qEV columns. a RNA concentration. b Representative bioanalyzer profiles of RNA isolated from the same donor and analyzed by RNA 6000 Pico Kit; the y-axis shows fluorescence units (FU) and the x-axis the nucleotide length (nt) of the RNA. Peaks at 25 nt is an internal standard. c Ratio of total amount of RNA (µg) to total amount of protein (µg). (mean ± SEM, n = 6; *p

Techniques Used: Isolation, Size-exclusion Chromatography, Concentration Assay, Fluorescence

8) Product Images from "Exosomal miRNAs from Peritoneum Lavage Fluid as Potential Prognostic Biomarkers of Peritoneal Metastasis in Gastric Cancer"

Article Title: Exosomal miRNAs from Peritoneum Lavage Fluid as Potential Prognostic Biomarkers of Peritoneal Metastasis in Gastric Cancer

Journal: PLoS ONE

doi: 10.1371/journal.pone.0130472

Exosomal miRNA expression in malignant ascites (MA), peritoneal lavage fluid (PLF), and cell culture media (CM). Variation and commonality between the samples were examined. Evaluation of total exosomal RNA used a Bioanalyzer 2100. The electropherograms show the size distribution (nucleotides, nt) and fluorescence intensity (FU) of total exosomal RNA isolated from CM, MA, and PLF. The lowest spike (around 25 nt) in each sample is the marker of the Agilent RNA 6000 Pico kit.
Figure Legend Snippet: Exosomal miRNA expression in malignant ascites (MA), peritoneal lavage fluid (PLF), and cell culture media (CM). Variation and commonality between the samples were examined. Evaluation of total exosomal RNA used a Bioanalyzer 2100. The electropherograms show the size distribution (nucleotides, nt) and fluorescence intensity (FU) of total exosomal RNA isolated from CM, MA, and PLF. The lowest spike (around 25 nt) in each sample is the marker of the Agilent RNA 6000 Pico kit.

Techniques Used: Expressing, Cell Culture, Fluorescence, Isolation, Marker

9) Product Images from "Ensuring good quality rna for quantitative real-time pcr isolated from renal proximal tubular cells using laser capture microdissection"

Article Title: Ensuring good quality rna for quantitative real-time pcr isolated from renal proximal tubular cells using laser capture microdissection

Journal: BMC Research Notes

doi: 10.1186/1756-0500-7-62

Total RNA concentrations from different number of renal cells. Total RNA concentrations were measured using Agilent 2100 Bioanalyzer with Agilent RNA 6000 Pico Kit. It was observed that 3000 renal cells yielded a total RNA concentration too low to be detected while 8000 and 18000 renal cells produced similar total RNA yields.
Figure Legend Snippet: Total RNA concentrations from different number of renal cells. Total RNA concentrations were measured using Agilent 2100 Bioanalyzer with Agilent RNA 6000 Pico Kit. It was observed that 3000 renal cells yielded a total RNA concentration too low to be detected while 8000 and 18000 renal cells produced similar total RNA yields.

Techniques Used: Concentration Assay, Produced

10) Product Images from "Cells release subpopulations of exosomes with distinct molecular and biological properties"

Article Title: Cells release subpopulations of exosomes with distinct molecular and biological properties

Journal: Scientific Reports

doi: 10.1038/srep22519

EV subpopulations have different RNA profiles. RNA from MV, LD-Exo and HD-Exo was extracted using Trizol and analyzed using capillary electrophoresis with the Agilent RNA 6000 Pico chip (left panel) and Agilent small RNA chip (right panel) on an Agilent 2100 Bioanalyzer®. The y-axis of the electropherograms represents fluorescence units (FU) and the x-axis represents the nucleotide length of the RNA (nt). Peaks at 25 nt (left panels) or at 4 nt (right panels) represent internal standards. Data shown are representative of two independent experiments.
Figure Legend Snippet: EV subpopulations have different RNA profiles. RNA from MV, LD-Exo and HD-Exo was extracted using Trizol and analyzed using capillary electrophoresis with the Agilent RNA 6000 Pico chip (left panel) and Agilent small RNA chip (right panel) on an Agilent 2100 Bioanalyzer®. The y-axis of the electropherograms represents fluorescence units (FU) and the x-axis represents the nucleotide length of the RNA (nt). Peaks at 25 nt (left panels) or at 4 nt (right panels) represent internal standards. Data shown are representative of two independent experiments.

Techniques Used: Electrophoresis, Chromatin Immunoprecipitation, Fluorescence

11) Product Images from "Pellet-free isolation of human and bovine milk extracellular vesicles by size-exclusion chromatography"

Article Title: Pellet-free isolation of human and bovine milk extracellular vesicles by size-exclusion chromatography

Journal: Journal of Extracellular Vesicles

doi: 10.1080/20013078.2017.1294340

Total RNA profiles for human and bovine MEV and cream fractions. Total RNA contained in fluff (red) and serum (green) MEV fractions were isolated from SEC F1/S1 peaks concentrated by reverse osmosis. Cream was sampled from the first centrifugation of raw milk. RNA extracts were analysed on Agilent RNA 6000 Pico chips using an Agilent Bioanalyzer. The total MEV RNA content was related to the initial start volume of milk (ng ml –1 milk). The peak at 25 nt is an internal standard. Abbreviations: FU, fluorescence units; nt, nucleotides; hF1, human fluff MEV; hS1, human milk serum MEV; bF1, bovine fluff MEV; bS1, bovine milk serum MEV.
Figure Legend Snippet: Total RNA profiles for human and bovine MEV and cream fractions. Total RNA contained in fluff (red) and serum (green) MEV fractions were isolated from SEC F1/S1 peaks concentrated by reverse osmosis. Cream was sampled from the first centrifugation of raw milk. RNA extracts were analysed on Agilent RNA 6000 Pico chips using an Agilent Bioanalyzer. The total MEV RNA content was related to the initial start volume of milk (ng ml –1 milk). The peak at 25 nt is an internal standard. Abbreviations: FU, fluorescence units; nt, nucleotides; hF1, human fluff MEV; hS1, human milk serum MEV; bF1, bovine fluff MEV; bS1, bovine milk serum MEV.

Techniques Used: Isolation, Size-exclusion Chromatography, Centrifugation, Fluorescence

12) Product Images from "Comparative evaluation of rRNA depletion procedures for the improved analysis of bacterial biofilm and mixed pathogen culture transcriptomes"

Article Title: Comparative evaluation of rRNA depletion procedures for the improved analysis of bacterial biofilm and mixed pathogen culture transcriptomes

Journal: Scientific Reports

doi: 10.1038/srep41114

Depletion of rRNA from RNA samples isolated from dual species P. aeruginosa / S. aureus cultures. A total of 2 μg of DNAse-treated RNA, isolated form a dual species P. aeruginosa PAO1 and S. aureus ATCC6538 culture, was subjected to treatment with the Illumina Ribo-Zero rRNA Removal Kit (Bacteria) or Ambion MICROBExpress™ Bacterial mRNA Enrichment Kit. Following rRNA depletion and ethanol/acetate precipitation and resuspension in equal volumes of water, the RNA was assessed using the Bioanalyzer RNA 6000 Pico kit. Representative electropherograms of ( A ) starting total RNA material and aliquots of the RNA samples that have been processed using the ( B ) MICROBExpress or ( C ) Ribo-Zero kits are shown. ( D ) Percent of rRNA contamination as reported by the Agilent Bioanalyzer 2100 Expert Software in RNA samples from single and dual species cultures of P. aeruginosa and/or S. aureus treated with the Ribo-Zero or MICROBExpress kits. Untreated input RNA (Total) or RNA processed via Ribo-Zero or MICROBExpress treatment was subjected to qPCR analysis of P. aeruginosa ( E , G ) or S. aureus ( F , H ) rRNA transcript abundance. ( E , F ) qPCR threshold cycle (Cq) for the indicated rRNA transcripts. ( G , H ) Copy numbers of P. aeruginosa or S. aureus 16S, 23 s, and 5S rRNA transcripts were calculated using respective qPCR standard curves. cDNA synthesis for the qPCR reactions was performed using 10 ng of indicated RNA samples as input. Experiments were repeated using two biological replicates. Error bars indicate standard deviation.
Figure Legend Snippet: Depletion of rRNA from RNA samples isolated from dual species P. aeruginosa / S. aureus cultures. A total of 2 μg of DNAse-treated RNA, isolated form a dual species P. aeruginosa PAO1 and S. aureus ATCC6538 culture, was subjected to treatment with the Illumina Ribo-Zero rRNA Removal Kit (Bacteria) or Ambion MICROBExpress™ Bacterial mRNA Enrichment Kit. Following rRNA depletion and ethanol/acetate precipitation and resuspension in equal volumes of water, the RNA was assessed using the Bioanalyzer RNA 6000 Pico kit. Representative electropherograms of ( A ) starting total RNA material and aliquots of the RNA samples that have been processed using the ( B ) MICROBExpress or ( C ) Ribo-Zero kits are shown. ( D ) Percent of rRNA contamination as reported by the Agilent Bioanalyzer 2100 Expert Software in RNA samples from single and dual species cultures of P. aeruginosa and/or S. aureus treated with the Ribo-Zero or MICROBExpress kits. Untreated input RNA (Total) or RNA processed via Ribo-Zero or MICROBExpress treatment was subjected to qPCR analysis of P. aeruginosa ( E , G ) or S. aureus ( F , H ) rRNA transcript abundance. ( E , F ) qPCR threshold cycle (Cq) for the indicated rRNA transcripts. ( G , H ) Copy numbers of P. aeruginosa or S. aureus 16S, 23 s, and 5S rRNA transcripts were calculated using respective qPCR standard curves. cDNA synthesis for the qPCR reactions was performed using 10 ng of indicated RNA samples as input. Experiments were repeated using two biological replicates. Error bars indicate standard deviation.

Techniques Used: Isolation, Software, Real-time Polymerase Chain Reaction, Standard Deviation

Quantitative and qualitative comparison of RNA recovered following rRNA depletion. A total of 4 μg of DNAse-treated RNA, isolated form 3-day-old P. aeruginosa PAO1 biofilms, was subjected to treatment with the Illumina Ribo-Zero rRNA Removal Kit (Bacteria), Ambion MICROBExpress™ Bacterial mRNA Enrichment Kit and the Life Technologies RiboMinus Transcriptome Isolation Kit, Bacteria. Following rRNA depletion and ethanol/acetate precipitation and resuspension in equal volumes of water, the RNA was assessed using Qubit fluorimetric quantitation with the Qubit RNA HS Assay Kit. Yields are reported as total RNA recovered ( A ) and as percentage of the input RNA ( B ). The RNA samples were also assessed using the Bioanalyzer RNA 6000 Pico kit. Representative electropherograms of ( C ) starting total RNA material and aliquots of the RNA samples that have been processed using the ( D ) MICROBExpress, ( E ) RiboMinus, or ( F ) Ribo-Zero kits are shown. Dashed lines indicate peaks corresponding to 16S and 23S RNA traces, which were detected in total RNA, MICROBExpress, and RiboMins samples. ( G ) The area of 16S and 23S rRNA peaks as percent of the total detected RNA was estimated, as determined using the 2100 Expert Software. RFU, relative fluorescence units. Experiments were repeated using three biological replicates.
Figure Legend Snippet: Quantitative and qualitative comparison of RNA recovered following rRNA depletion. A total of 4 μg of DNAse-treated RNA, isolated form 3-day-old P. aeruginosa PAO1 biofilms, was subjected to treatment with the Illumina Ribo-Zero rRNA Removal Kit (Bacteria), Ambion MICROBExpress™ Bacterial mRNA Enrichment Kit and the Life Technologies RiboMinus Transcriptome Isolation Kit, Bacteria. Following rRNA depletion and ethanol/acetate precipitation and resuspension in equal volumes of water, the RNA was assessed using Qubit fluorimetric quantitation with the Qubit RNA HS Assay Kit. Yields are reported as total RNA recovered ( A ) and as percentage of the input RNA ( B ). The RNA samples were also assessed using the Bioanalyzer RNA 6000 Pico kit. Representative electropherograms of ( C ) starting total RNA material and aliquots of the RNA samples that have been processed using the ( D ) MICROBExpress, ( E ) RiboMinus, or ( F ) Ribo-Zero kits are shown. Dashed lines indicate peaks corresponding to 16S and 23S RNA traces, which were detected in total RNA, MICROBExpress, and RiboMins samples. ( G ) The area of 16S and 23S rRNA peaks as percent of the total detected RNA was estimated, as determined using the 2100 Expert Software. RFU, relative fluorescence units. Experiments were repeated using three biological replicates.

Techniques Used: Isolation, Quantitation Assay, RNA HS Assay, Software, Fluorescence

rRNA depletion treatments of RNA derived from planktonic P. aeruginosa PAO1 and Staphylococcus aureus ATCC6538 cells. A total of 2 μg of DNAse-treated RNA, isolated from exponential phase P. aeruginosa PAO1 or Staphylococcus aureus ATCC6538 cells, was subjected to treatment with the Illumina Ribo-Zero rRNA Removal Kit (Bacteria) or Ambion MICROBExpress™ Bacterial mRNA Enrichment Kit. Following rRNA depletion and ethanol/acetate precipitation and resuspension in equal volumes of water, the RNA was assessed using the Bioanalyzer RNA 6000 Pico kit. Representative electropherograms of starting total RNA material and aliquots of the RNA samples that have been processed using the MICROBExpress or Ribo-Zero kits are shown for the P. aeruginosa ( A ) and S. aureus ( B ) samples. The samples were subsequently subjected to qPCR analysis of rRNA transcript abundance, with qPCR threshold cycle (Cq) for the indicated rRNA transcripts shown for P. aeruginosa ( C ) and S. aureus ( E ). Copy numbers of P. aeruginosa ( D ) and S. aureus ( F ) 16S, 23 s, and 5S rRNA transcripts were calculated using respective qPCR standard curves. cDNA synthesis for the qPCR reactions was performed using 10 ng of indicated RNA samples as input. Experiments were repeated using two biological replicates. Error bars indicate standard deviation.
Figure Legend Snippet: rRNA depletion treatments of RNA derived from planktonic P. aeruginosa PAO1 and Staphylococcus aureus ATCC6538 cells. A total of 2 μg of DNAse-treated RNA, isolated from exponential phase P. aeruginosa PAO1 or Staphylococcus aureus ATCC6538 cells, was subjected to treatment with the Illumina Ribo-Zero rRNA Removal Kit (Bacteria) or Ambion MICROBExpress™ Bacterial mRNA Enrichment Kit. Following rRNA depletion and ethanol/acetate precipitation and resuspension in equal volumes of water, the RNA was assessed using the Bioanalyzer RNA 6000 Pico kit. Representative electropherograms of starting total RNA material and aliquots of the RNA samples that have been processed using the MICROBExpress or Ribo-Zero kits are shown for the P. aeruginosa ( A ) and S. aureus ( B ) samples. The samples were subsequently subjected to qPCR analysis of rRNA transcript abundance, with qPCR threshold cycle (Cq) for the indicated rRNA transcripts shown for P. aeruginosa ( C ) and S. aureus ( E ). Copy numbers of P. aeruginosa ( D ) and S. aureus ( F ) 16S, 23 s, and 5S rRNA transcripts were calculated using respective qPCR standard curves. cDNA synthesis for the qPCR reactions was performed using 10 ng of indicated RNA samples as input. Experiments were repeated using two biological replicates. Error bars indicate standard deviation.

Techniques Used: Derivative Assay, Isolation, Real-time Polymerase Chain Reaction, Standard Deviation

13) Product Images from "A total transcriptome profiling method for plasma-derived extracellular vesicles: applications for liquid biopsies"

Article Title: A total transcriptome profiling method for plasma-derived extracellular vesicles: applications for liquid biopsies

Journal: Scientific Reports

doi: 10.1038/s41598-017-14264-5

Bioanalyzer electropherogram analysis of fragmentation time-points of leukocyte RNA. Arbitrary fluorescence units (FU) are plotted as a function of RNA size in nucleotides (nt). ( A ) Analysis of fragmentation time-points 15–180 min with Agilent RNA 6000 Pico Kit shows the expected ribosomal RNA peaks in non-fragmented sample (in red), whereas after all fragmentation time-points the majority of RNAs are below 200 nt in size. ( B ) Analysis of fragmentation time-points from 45 to 420 min with Agilent Small RNA Kit shows that the majority of RNAs are smaller than 40 nt in size for all time-points, and that a plateau is reached after approximately 180 min, as no further reduction in size is observed with longer fragmentation times. For the longer fragmentation periods, reduced amounts of RNA (marked with a star) were used to better simulate the enzymatic kinetics in the presence of less (but still detectable) RNA, and the plateau region is still the same.
Figure Legend Snippet: Bioanalyzer electropherogram analysis of fragmentation time-points of leukocyte RNA. Arbitrary fluorescence units (FU) are plotted as a function of RNA size in nucleotides (nt). ( A ) Analysis of fragmentation time-points 15–180 min with Agilent RNA 6000 Pico Kit shows the expected ribosomal RNA peaks in non-fragmented sample (in red), whereas after all fragmentation time-points the majority of RNAs are below 200 nt in size. ( B ) Analysis of fragmentation time-points from 45 to 420 min with Agilent Small RNA Kit shows that the majority of RNAs are smaller than 40 nt in size for all time-points, and that a plateau is reached after approximately 180 min, as no further reduction in size is observed with longer fragmentation times. For the longer fragmentation periods, reduced amounts of RNA (marked with a star) were used to better simulate the enzymatic kinetics in the presence of less (but still detectable) RNA, and the plateau region is still the same.

Techniques Used: Fluorescence

14) Product Images from "Detection of circulating miRNAs: comparative analysis of extracellular vesicle-incorporated miRNAs and cell-free miRNAs in whole plasma of prostate cancer patients"

Article Title: Detection of circulating miRNAs: comparative analysis of extracellular vesicle-incorporated miRNAs and cell-free miRNAs in whole plasma of prostate cancer patients

Journal: BMC Cancer

doi: 10.1186/s12885-017-3737-z

Effects of proteinase K and RNase A treatment on the relative quantity of EV-incorporated miRNAs and RNA profiles in whole plasma and EVs. a RT-qPCR analysis of miRNA levels in EVs treated with RNase A alone or with a combination of proteinase K and RNase A relatively to untreated EVs. Bars show the mean percentage in EVs from 3 healthy individuals. b A representative RNA profile from whole plasma and EVs treated with proteinase K and RNase A obtained by Bioanlyzer RNA 6000 Pico chip
Figure Legend Snippet: Effects of proteinase K and RNase A treatment on the relative quantity of EV-incorporated miRNAs and RNA profiles in whole plasma and EVs. a RT-qPCR analysis of miRNA levels in EVs treated with RNase A alone or with a combination of proteinase K and RNase A relatively to untreated EVs. Bars show the mean percentage in EVs from 3 healthy individuals. b A representative RNA profile from whole plasma and EVs treated with proteinase K and RNase A obtained by Bioanlyzer RNA 6000 Pico chip

Techniques Used: Quantitative RT-PCR, Chromatin Immunoprecipitation

15) Product Images from "Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes"

Article Title: Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes

Journal: BMC Genomics

doi: 10.1186/s12864-015-1225-x

Experimental infection, parasite isolation, and RNA preparation. A) Immunofluorescence assay using sheep immune serum against T. gondii revealed numerous infected enterocytes. Nuclei are counterstained with DAPI. Shown in the top panel is a 20x magnification of a section of the small intestine (bar = 100 μM) where villi are visible. The bottom panel at 100x magnification shows a schizont containing several merozoites (scale bar = 5 μm). B) Enterocytes containing CZ-strain merozoite stages were stripped away selectively, leaving the villus structure and the cells of the lamina propria intact. Histology section (Hematoxilin Eosin stained) showing stripped villi at day 5 post infection. C) Microscopic examination of parasites in the detergent washed preparation showed only merozoite stages. D) Quality control of total RNA extracted from parasite preparations separated on an Agilent RNA 6000 Pico Chip. The bands generated by host 28S/18S ribosomal RNA (arrowheads) and parasite 26S/18S ribosomal RNA (arrows) as well as a size marker are indicated. The samples analyzed were: raw, unprocessed material from scraped intestinal lining; Tween 80, material that was syringe-passaged and washed twice with PBS/0.05% Tween 80; Percoll, highly enriched parasite fraction after detergent treatment and Percoll gradient centrifugation; Tachy, RNA prepared from tachyzoites grown in cell culture with human foreskin fibroblasts as host cells.
Figure Legend Snippet: Experimental infection, parasite isolation, and RNA preparation. A) Immunofluorescence assay using sheep immune serum against T. gondii revealed numerous infected enterocytes. Nuclei are counterstained with DAPI. Shown in the top panel is a 20x magnification of a section of the small intestine (bar = 100 μM) where villi are visible. The bottom panel at 100x magnification shows a schizont containing several merozoites (scale bar = 5 μm). B) Enterocytes containing CZ-strain merozoite stages were stripped away selectively, leaving the villus structure and the cells of the lamina propria intact. Histology section (Hematoxilin Eosin stained) showing stripped villi at day 5 post infection. C) Microscopic examination of parasites in the detergent washed preparation showed only merozoite stages. D) Quality control of total RNA extracted from parasite preparations separated on an Agilent RNA 6000 Pico Chip. The bands generated by host 28S/18S ribosomal RNA (arrowheads) and parasite 26S/18S ribosomal RNA (arrows) as well as a size marker are indicated. The samples analyzed were: raw, unprocessed material from scraped intestinal lining; Tween 80, material that was syringe-passaged and washed twice with PBS/0.05% Tween 80; Percoll, highly enriched parasite fraction after detergent treatment and Percoll gradient centrifugation; Tachy, RNA prepared from tachyzoites grown in cell culture with human foreskin fibroblasts as host cells.

Techniques Used: Infection, Isolation, Immunofluorescence, Staining, Chromatin Immunoprecipitation, Generated, Marker, Gradient Centrifugation, Cell Culture

16) Product Images from "Presence of Circulating miR-145, miR-155, and miR-382 in Exosomes Isolated from Serum of Breast Cancer Patients and Healthy Donors"

Article Title: Presence of Circulating miR-145, miR-155, and miR-382 in Exosomes Isolated from Serum of Breast Cancer Patients and Healthy Donors

Journal: Disease Markers

doi: 10.1155/2019/6852917

Quality of small RNA isolated from exosomes. Representative electropherogram of RNA quality extracted from exosomes derived of (a) healthy donors and (b) BC patients. All samples were analyzed by the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA) with the RNA 6000 Pico Kit (Agilent Technologies). The Y -axis represents fluorescence units (FU) and the X -axis shows nucleotides (nt). The peak observed between 25 nt and 200 nt correspond to the small RNA region. Peaks in the regions around 1500 nt and 4000 nt relative to 18S and 28S ribosomal RNAs, respectively, were not or barely detected. The peak of 25 nt refers to the marker. (c) Virtual gel of the data is shown in the electropherograms.
Figure Legend Snippet: Quality of small RNA isolated from exosomes. Representative electropherogram of RNA quality extracted from exosomes derived of (a) healthy donors and (b) BC patients. All samples were analyzed by the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA) with the RNA 6000 Pico Kit (Agilent Technologies). The Y -axis represents fluorescence units (FU) and the X -axis shows nucleotides (nt). The peak observed between 25 nt and 200 nt correspond to the small RNA region. Peaks in the regions around 1500 nt and 4000 nt relative to 18S and 28S ribosomal RNAs, respectively, were not or barely detected. The peak of 25 nt refers to the marker. (c) Virtual gel of the data is shown in the electropherograms.

Techniques Used: Isolation, Derivative Assay, Fluorescence, Marker

17) Product Images from "The response of Synechococcus sp. PCC 7002 to micro-/nano polyethylene particles - Investigation of a key anthropogenic stressor"

Article Title: The response of Synechococcus sp. PCC 7002 to micro-/nano polyethylene particles - Investigation of a key anthropogenic stressor

Journal: PLoS ONE

doi: 10.1371/journal.pone.0232745

Electropherograms generated by Agilent RNA 6000. Electropherograms generated from total RNA extractions from  Synechococcus sp .  7002  bacterial cells exposed to polyethylene microparticles and nanoparticles after 0, 5 and 10 days. A). Total RNA sample after 0 days exposure to polyethylene microparticles B) Total RNA sample after 0 days exposure to polyethylene nanoparticles C) Total RNA sample after 5 days exposure to polyethylene microparticles D) Total RNA sample after 5 days exposure to polyethylene nanoparticles. E) Total RNA sample after 10 days exposure to polyethylene microparticles F) Total RNA sample after 10 days exposure to polyethylene nanoparticles G) Control sample exposed to 0.1% Tween solution.
Figure Legend Snippet: Electropherograms generated by Agilent RNA 6000. Electropherograms generated from total RNA extractions from Synechococcus sp . 7002 bacterial cells exposed to polyethylene microparticles and nanoparticles after 0, 5 and 10 days. A). Total RNA sample after 0 days exposure to polyethylene microparticles B) Total RNA sample after 0 days exposure to polyethylene nanoparticles C) Total RNA sample after 5 days exposure to polyethylene microparticles D) Total RNA sample after 5 days exposure to polyethylene nanoparticles. E) Total RNA sample after 10 days exposure to polyethylene microparticles F) Total RNA sample after 10 days exposure to polyethylene nanoparticles G) Control sample exposed to 0.1% Tween solution.

Techniques Used: Generated

Related Articles

Isolation:

Article Title: Gene expression analysis on small numbers of invasive cells collected by chemotaxis from primary mammary tumors of the mouse
Article Snippet: .. To verify successful RNA isolation and the intactness of the RNA samples, the RNA 6000 Pico kit and Agilent 2100 Bioanalyzer (Agilent Technologies) were used. ..

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    Agilent technologies rna 6000 pico labchip kit
    Results of RNA-integrity-testing by a Agilent Bioanalyzer 2100 using the <t>RNA</t> 6000 Pico <t>LabChip</t> kit. Lane 1 and 2 are HOPE-fixed materials and lane 3 is FFPE.
    Rna 6000 Pico Labchip Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 91/100, based on 88 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies rna 6000 pico kit
    Characterization of exosomal RNA (esRNA) extracted from purified nanovesicles from fresh human aqueous humor. The esRNA was evaluated using Agilent Bioanalyzer 2100 with Agilent <t>RNA</t> 6000 Pico Kit. A) Shows the RNA ladder and B) displays extracted esRNA from one of the human aqueous humor samples. The size of extracted esRNA was less than 200 nucleotides (nt), mostly around 25 nt. C) shows the miRNAs that were identified via small RNA sequencing using Illumina MiSeq sequencing system. The height of each bar indicated the number of sequence reads with perfect match to mature miRNA.
    Rna 6000 Pico Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 94/100, based on 73 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Results of RNA-integrity-testing by a Agilent Bioanalyzer 2100 using the RNA 6000 Pico LabChip kit. Lane 1 and 2 are HOPE-fixed materials and lane 3 is FFPE.

    Journal: Diagnostic Pathology

    Article Title: Enhanced molecular analyses by combination of the HOPE-technique and laser microdissection

    doi: 10.1186/1746-1596-1-2

    Figure Lengend Snippet: Results of RNA-integrity-testing by a Agilent Bioanalyzer 2100 using the RNA 6000 Pico LabChip kit. Lane 1 and 2 are HOPE-fixed materials and lane 3 is FFPE.

    Article Snippet: Of each RNA sample 1 μl was tested in an Agilent Bioanalyzer 2100 using the RNA 6000 Pico LabChip kit (Agilent, Waldbronn, Germany, #5065-4473) to determine the RNA integrity.

    Techniques: Formalin-fixed Paraffin-Embedded

    Integrity of the RNA samples. Each bar depicts the arithmetic mean of the RNA integrity numbers (RIN) measured for the 10 whole RNA samples, which were used for the microarray hybridisation per cell population and per time point. The error bars indicate standard deviations. This quality control was carried out using the Agilent Bioanalyzer 2100 and the RNA 6000 Pico LabChip kit (Agilent Technologies, Waldbronn, Germany).

    Journal: Scientific Data

    Article Title: Microarray data of transcriptome shifts in blood cell subsets during S1P receptor modulator therapy

    doi: 10.1038/sdata.2018.145

    Figure Lengend Snippet: Integrity of the RNA samples. Each bar depicts the arithmetic mean of the RNA integrity numbers (RIN) measured for the 10 whole RNA samples, which were used for the microarray hybridisation per cell population and per time point. The error bars indicate standard deviations. This quality control was carried out using the Agilent Bioanalyzer 2100 and the RNA 6000 Pico LabChip kit (Agilent Technologies, Waldbronn, Germany).

    Article Snippet: The integrity of the RNA samples was checked using an Agilent Bioanalyzer 2100 with the RNA 6000 Pico LabChip kit (both from Agilent Technologies, Waldbronn, Germany).

    Techniques: Microarray, Hybridization

    Characterization of exosomal RNA (esRNA) extracted from purified nanovesicles from fresh human aqueous humor. The esRNA was evaluated using Agilent Bioanalyzer 2100 with Agilent RNA 6000 Pico Kit. A) Shows the RNA ladder and B) displays extracted esRNA from one of the human aqueous humor samples. The size of extracted esRNA was less than 200 nucleotides (nt), mostly around 25 nt. C) shows the miRNAs that were identified via small RNA sequencing using Illumina MiSeq sequencing system. The height of each bar indicated the number of sequence reads with perfect match to mature miRNA.

    Journal: Experimental eye research

    Article Title: Human Aqueous Humor Exosomes

    doi: 10.1016/j.exer.2015.01.019

    Figure Lengend Snippet: Characterization of exosomal RNA (esRNA) extracted from purified nanovesicles from fresh human aqueous humor. The esRNA was evaluated using Agilent Bioanalyzer 2100 with Agilent RNA 6000 Pico Kit. A) Shows the RNA ladder and B) displays extracted esRNA from one of the human aqueous humor samples. The size of extracted esRNA was less than 200 nucleotides (nt), mostly around 25 nt. C) shows the miRNAs that were identified via small RNA sequencing using Illumina MiSeq sequencing system. The height of each bar indicated the number of sequence reads with perfect match to mature miRNA.

    Article Snippet: Quality of extracted esRNA was evaluated using Agilent Bioanalyzer 2100 with Agilent RNA 6000 Pico Kit.

    Techniques: Purification, RNA Sequencing Assay, Sequencing

    RNA profiles from exosomes from bulk cells (left) and CSCs (right) exosomes obtained by Agilent 2100 Bioanalyzer The electropherograms shows the size in distribution of the nucleotides (nt) and fluorescence intensity (FU) of Total RNA by Agilent RNA 6000 Pico Kit (A) and small RNAs after enrichment by the Agilent Small RNA Kit (B) All the electropherograms correspond to the sample of the same patient. The inserts show a representative image from the cultures under study.

    Journal: Oncotarget

    Article Title: Exosomes from bulk and stem cells from human prostate cancer have a differential microRNA content that contributes cooperatively over local and pre-metastatic niche

    doi: 10.18632/oncotarget.6540

    Figure Lengend Snippet: RNA profiles from exosomes from bulk cells (left) and CSCs (right) exosomes obtained by Agilent 2100 Bioanalyzer The electropherograms shows the size in distribution of the nucleotides (nt) and fluorescence intensity (FU) of Total RNA by Agilent RNA 6000 Pico Kit (A) and small RNAs after enrichment by the Agilent Small RNA Kit (B) All the electropherograms correspond to the sample of the same patient. The inserts show a representative image from the cultures under study.

    Article Snippet: The quality of the total RNA was assessed by Agilent 2100 Bioanalyzer using an RNA 6000 Pico Kit (Agilent Technologies).

    Techniques: Fluorescence