Structured Review

Agilent technologies rna 6000 nano labchip kit
Extracting High Quality RNA from Bone in a Single Step . (A) Isolated liver RNA was incubated with RNase free beads (lane 1) or untreated beads (lane 2) provided by the manufacturer (Next Advance) for four hours before analyzing the 18S and 28S rRNA bands using agarose gel chromatography. Subsequent experiments with the Bullet Blender were carried out using untreated beads. (B) Bone RNA was homogenized in near freezing conditions using the Bullet Blender centrifuge (lanes 2-4) or a Polytron (lanes 5-6). The results are compared to bone RNA isolated using standard homogenization conditions (lane 1) and intact liver RNA that was previously isolated (control). (C) The RNA Integrity Number (RIN) for RNA homogenized in near freezing conditions was determined using the Agilent <t>RNA</t> 6000 <t>Nano</t> <t>LabChip</t> Kit and the Agilent 2100 Bioanalyzer. RNA with a RIN = 7 or greater is suitable for microarray analysis of gene expression. (D) The maximum RIN for the two-step approach (lane 2) is compared to the maximum RIN obtained in the one-step approach (lane 3) and each RIN is compared to the RIN of the degraded RNA sample (lane 1) shown in the agarose gel in lane 1, Figure 2B. (E) The electropherograms associated with the samples shown in (D).
Rna 6000 Nano Labchip Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 122 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rna 6000 nano labchip kit/product/Agilent technologies
Average 99 stars, based on 122 article reviews
Price from $9.99 to $1999.99
rna 6000 nano labchip kit - by Bioz Stars, 2019-09
99/100 stars

Images

1) Product Images from "An improved method for isolation of RNA from bone"

Article Title: An improved method for isolation of RNA from bone

Journal: BMC Biotechnology

doi: 10.1186/1472-6750-12-5

Extracting High Quality RNA from Bone in a Single Step . (A) Isolated liver RNA was incubated with RNase free beads (lane 1) or untreated beads (lane 2) provided by the manufacturer (Next Advance) for four hours before analyzing the 18S and 28S rRNA bands using agarose gel chromatography. Subsequent experiments with the Bullet Blender were carried out using untreated beads. (B) Bone RNA was homogenized in near freezing conditions using the Bullet Blender centrifuge (lanes 2-4) or a Polytron (lanes 5-6). The results are compared to bone RNA isolated using standard homogenization conditions (lane 1) and intact liver RNA that was previously isolated (control). (C) The RNA Integrity Number (RIN) for RNA homogenized in near freezing conditions was determined using the Agilent RNA 6000 Nano LabChip Kit and the Agilent 2100 Bioanalyzer. RNA with a RIN = 7 or greater is suitable for microarray analysis of gene expression. (D) The maximum RIN for the two-step approach (lane 2) is compared to the maximum RIN obtained in the one-step approach (lane 3) and each RIN is compared to the RIN of the degraded RNA sample (lane 1) shown in the agarose gel in lane 1, Figure 2B. (E) The electropherograms associated with the samples shown in (D).
Figure Legend Snippet: Extracting High Quality RNA from Bone in a Single Step . (A) Isolated liver RNA was incubated with RNase free beads (lane 1) or untreated beads (lane 2) provided by the manufacturer (Next Advance) for four hours before analyzing the 18S and 28S rRNA bands using agarose gel chromatography. Subsequent experiments with the Bullet Blender were carried out using untreated beads. (B) Bone RNA was homogenized in near freezing conditions using the Bullet Blender centrifuge (lanes 2-4) or a Polytron (lanes 5-6). The results are compared to bone RNA isolated using standard homogenization conditions (lane 1) and intact liver RNA that was previously isolated (control). (C) The RNA Integrity Number (RIN) for RNA homogenized in near freezing conditions was determined using the Agilent RNA 6000 Nano LabChip Kit and the Agilent 2100 Bioanalyzer. RNA with a RIN = 7 or greater is suitable for microarray analysis of gene expression. (D) The maximum RIN for the two-step approach (lane 2) is compared to the maximum RIN obtained in the one-step approach (lane 3) and each RIN is compared to the RIN of the degraded RNA sample (lane 1) shown in the agarose gel in lane 1, Figure 2B. (E) The electropherograms associated with the samples shown in (D).

Techniques Used: Isolation, Incubation, Agarose Gel Electrophoresis, Chromatography, Homogenization, Microarray, Expressing

Related Articles

Amplification:

Article Title: Substantial Dysregulation of miRNA Passenger Strands Underlies the Vascular Response to Injury
Article Snippet: The RNAs were processed through miRNEasy Mini Kit (Qiagen) following the manufacturer’s instructions, treated with DNAse 1 (amplification grade; Sigma, St. Louis, MO, USA) in order to eliminate genomic DNA contamination and quantified using a NanoDrop ND-1000 Spectrophotometer (Nano-Drop Technologies, Wilmington, DE, USA). .. RNA integrity was assessed using the RNA 6000 Nano LabChip kit (Agilent Technologies, Santa Clara, CA, USA).

Article Title: Lysozyme association with circulating RNA, extracellular vesicles, and chronic stress
Article Snippet: RNA quantity, purity, and integrity were assessed by a NanoDropTM 1000 spectrophotometer (Wilmington, DE) or by 2100 Bioanalyzer using the RNA 6000 Nano LabChip kit (Agilent Technologies, Santa Clara, CA). .. RNA quantity, purity, and integrity were assessed by a NanoDropTM 1000 spectrophotometer (Wilmington, DE) or by 2100 Bioanalyzer using the RNA 6000 Nano LabChip kit (Agilent Technologies, Santa Clara, CA).

Article Title: The miRNA-Mediated Post-Transcriptional Regulation of Maize in Response to High Temperature
Article Snippet: The quality and purity of the total RNA was analyzed using Bioanalyzer 2100 and an RNA 6000 Nano LabChip Kit (Agilent, Santa Clara, CA, USA). .. In brief, 1 μg of total RNA was ligated to RNA-DNA chimeric oligonucleotide adaptors and converted to cDNA by RT-PCR.

Synthesized:

Article Title: Lysozyme association with circulating RNA, extracellular vesicles, and chronic stress
Article Snippet: RNA quantity, purity, and integrity were assessed by a NanoDropTM 1000 spectrophotometer (Wilmington, DE) or by 2100 Bioanalyzer using the RNA 6000 Nano LabChip kit (Agilent Technologies, Santa Clara, CA). .. RNA quantity, purity, and integrity were assessed by a NanoDropTM 1000 spectrophotometer (Wilmington, DE) or by 2100 Bioanalyzer using the RNA 6000 Nano LabChip kit (Agilent Technologies, Santa Clara, CA).

Quantitative RT-PCR:

Article Title: High throughput deep sequencing reveals the important roles of microRNAs during sweetpotato storage at chilling temperature
Article Snippet: The quantity and purity of total RNA were analyzed with a Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA) with RIN number > 7.0. .. Samples with 1–5 weeks of treatments for ATS and LTS were used for measuring the contents of MDA, starch and sucrose, and the activities of POD and SOD.

Microarray:

Article Title: Global Gene-expression Analysis of the Response of Salmonella Enteritidis to Egg White Exposure Reveals Multiple Egg White-imposed Stress Responses
Article Snippet: The evaluation of RNA quantity and quality was assessed spectrophotometrically by measuring the ultraviolet absorbance profile (NanoDrop, NanoDrop Technologies, Inc., Rockland, Wilmington, DE, USA) at 230, 260, and 280 nm. .. For the microarray experiment, additional analysis for RNA integrity was performed using a RNA 6000 Nano LabChip kit (2100 Bioanalyzer, Agilent Technologies, Santa Clara, CA, USA) (Mueller et al., ). .. The RNA samples were then DNase treated using a DNA-free kit (Ambion, Austin, TX, USA) according to the manufacturer's instructions.

Incubation:

Article Title: Global Gene-expression Analysis of the Response of Salmonella Enteritidis to Egg White Exposure Reveals Multiple Egg White-imposed Stress Responses
Article Snippet: After incubation, cells were centrifuged at 10,000 × g at 4°C for 5 min, pellets were immediately frozen in liquid nitrogen and stored at −80°C. .. For the microarray experiment, additional analysis for RNA integrity was performed using a RNA 6000 Nano LabChip kit (2100 Bioanalyzer, Agilent Technologies, Santa Clara, CA, USA) (Mueller et al., ).

Expressing:

Article Title: Lysozyme association with circulating RNA, extracellular vesicles, and chronic stress
Article Snippet: Paragraph title: Blood RNA collection and gene expression profiling ... RNA quantity, purity, and integrity were assessed by a NanoDropTM 1000 spectrophotometer (Wilmington, DE) or by 2100 Bioanalyzer using the RNA 6000 Nano LabChip kit (Agilent Technologies, Santa Clara, CA).

Article Title: High throughput deep sequencing reveals the important roles of microRNAs during sweetpotato storage at chilling temperature
Article Snippet: The quantity and purity of total RNA were analyzed with a Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA) with RIN number > 7.0. .. Samples with 4 weeks of treatment were used for sequencing and qRT-PCR analysis.

Hybridization:

Article Title: Lysozyme association with circulating RNA, extracellular vesicles, and chronic stress
Article Snippet: RNA quantity, purity, and integrity were assessed by a NanoDropTM 1000 spectrophotometer (Wilmington, DE) or by 2100 Bioanalyzer using the RNA 6000 Nano LabChip kit (Agilent Technologies, Santa Clara, CA). .. RNA quantity, purity, and integrity were assessed by a NanoDropTM 1000 spectrophotometer (Wilmington, DE) or by 2100 Bioanalyzer using the RNA 6000 Nano LabChip kit (Agilent Technologies, Santa Clara, CA).

Generated:

Article Title: Integrated analysis of high-throughput sequencing data shows abscisic acid-responsive genes and miRNAs in strawberry receptacle fruit ripening
Article Snippet: Before intrasample pooling, individual total RNA quantity and concentration were measured on a Bioanalyzer 2100 and with an RNA 6000 Nano LabChip Kit (Agilent, CA, USA) with RNA integrity number (RIN) > 8.0. .. After pooling, 14 RNA samples were prepared for cDNA library construction.

Polymerase Chain Reaction:

Article Title: The miRNA-Mediated Post-Transcriptional Regulation of Maize in Response to High Temperature
Article Snippet: The quality and purity of the total RNA was analyzed using Bioanalyzer 2100 and an RNA 6000 Nano LabChip Kit (Agilent, Santa Clara, CA, USA). .. In brief, 1 μg of total RNA was ligated to RNA-DNA chimeric oligonucleotide adaptors and converted to cDNA by RT-PCR.

Reverse Transcription Polymerase Chain Reaction:

Article Title: The miRNA-Mediated Post-Transcriptional Regulation of Maize in Response to High Temperature
Article Snippet: The quality and purity of the total RNA was analyzed using Bioanalyzer 2100 and an RNA 6000 Nano LabChip Kit (Agilent, Santa Clara, CA, USA). .. Approximately 1 μg of total RNA was used for each sRNA library preparation according to the TruSeq Small RNA Sample Prep Kit protocol (Illumina, San Diego, CA, USA).

RNA Sequencing Assay:

Article Title: A large family of Dscam genes with tandemly arrayed 5′ cassettes in Chelicerata
Article Snippet: Paragraph title: RNA-seq ... The total RNA quantity and purity were analysed using a Bioanalyser 2100 and RNA 6000 Nano LabChip Kit (Agilent, Santa Clara, CA, USA) with RNA integrity number > 7.0.

Article Title: The roles of TRIO and F-actin-binding protein in glioblastoma cells
Article Snippet: Paragraph title: RNA sequencing and RNA-Seq data analysis ... The total RNA quantity and purity were analysis of Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent Technologies, Inc., Santa Clara, CA, USA).

Article Title: Integrated analysis of high-throughput sequencing data shows abscisic acid-responsive genes and miRNAs in strawberry receptacle fruit ripening
Article Snippet: Before intrasample pooling, individual total RNA quantity and concentration were measured on a Bioanalyzer 2100 and with an RNA 6000 Nano LabChip Kit (Agilent, CA, USA) with RNA integrity number (RIN) > 8.0. .. After pooling, 14 RNA samples were prepared for cDNA library construction.

Article Title: Transcriptome Analysis Reveals Cotton (Gossypium hirsutum) Genes That Are Differentially Expressed in Cadmium Stress Tolerance
Article Snippet: Paragraph title: 4.3. RNA Extraction, cDNA Library Construction, and RNA-Seq ... Total RNA quantity and purity were measured with Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, Santa Clara, CA, USA) with RIN number > 7, followed by gel extraction with 1% agarose gel electrophoresis.

Mutagenesis:

Article Title: Key Genes and Pathways Associated With Inner Ear Malformation in SOX10 p.R109W Mutation Pigs
Article Snippet: Otic vesicles were obtained from five WT and five mutant embryos at E28. .. The quantity and purity of the collected total RNA were analyzed with a Bioanalyzer 2200 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA).

Isolation:

Article Title: An improved method for isolation of RNA from bone
Article Snippet: RNA Quality and Yield RNA yield was determined by the absorbance at 260 nm (Table ) and using the Agilent RNA 6000 Nano LabChip Kit and the Agilent 2100 Bioanalyzer (Agilent Technologies). .. RNA Quality and Yield RNA yield was determined by the absorbance at 260 nm (Table ) and using the Agilent RNA 6000 Nano LabChip Kit and the Agilent 2100 Bioanalyzer (Agilent Technologies).

Article Title: Substantial Dysregulation of miRNA Passenger Strands Underlies the Vascular Response to Injury
Article Snippet: Paragraph title: 2.2. Vessel Storage and RNA Isolation from the Pig Vein Graft Model and Stent Model ... RNA integrity was assessed using the RNA 6000 Nano LabChip kit (Agilent Technologies, Santa Clara, CA, USA).

Article Title: Lysozyme association with circulating RNA, extracellular vesicles, and chronic stress
Article Snippet: 2.2 Peripheral whole blood was collected in PAXgene® RNA tubes and total RNA was isolated with PAXgene® Blood miRNA kit as per the manufacturer's instructions. .. RNA quantity, purity, and integrity were assessed by a NanoDropTM 1000 spectrophotometer (Wilmington, DE) or by 2100 Bioanalyzer using the RNA 6000 Nano LabChip kit (Agilent Technologies, Santa Clara, CA).

Article Title: Characterisation of the Whole Blood mRNA Transcriptome in Holstein-Friesian and Jersey Calves in Response to Gradual Weaning
Article Snippet: Total RNA was extracted from whole blood using the TempusTM Spin RNA Isolation Reagent Kit (Applied Biosystems; Foster City, California, USA), according to manufacturer’s instructions. .. The quality of the extracted RNA was examined with the Agilent 2100 Bioanalyser (Agilent Technologies Ireland Ltd; Dublin, Ireland) using the RNA 6000 Nano LabChip kit (Agilent Technologies Ireland Ltd; Dublin, Ireland).

Article Title: Global Gene-expression Analysis of the Response of Salmonella Enteritidis to Egg White Exposure Reveals Multiple Egg White-imposed Stress Responses
Article Snippet: Paragraph title: RNA isolation and synthesis of cDNA ... For the microarray experiment, additional analysis for RNA integrity was performed using a RNA 6000 Nano LabChip kit (2100 Bioanalyzer, Agilent Technologies, Santa Clara, CA, USA) (Mueller et al., ).

Article Title: Antisense RNA transcripts in the blood may be novel diagnostic markers for colorectal cancer
Article Snippet: Blood samples were collected from the patients using PAXgene Vacutainer tubes (BD Biosciences, Franklin Lakes, NJ, USA) and subjected to RNA isolation and extraction using a PAXgene Blood RNA Isolation kit (Qiagen, Inc., Valencia, CA, USA), following the manufacturer's instructions. .. The quantity of total RNA obtained was determined using a NanoDrop Spectrophotometer (NanoDrop Technologies; Thermo Fisher Scientific, Inc., Waltham, MA, USA) according to the 280/260 nm absorbance ratio, and RNA integrity was evaluated using an RNA 6000 Nano LabChip kit (Agilent Technologies, Inc., Santa Clara, CA, USA) and an Agilent 2100 Bioanalyzer (Agilent Technologies, Inc.).

Article Title: Integrated analysis of high-throughput sequencing data shows abscisic acid-responsive genes and miRNAs in strawberry receptacle fruit ripening
Article Snippet: Total RNA was isolated using RNAiso Plus (TaKaRa, Japan) following the manufacturer’s protocol. .. Before intrasample pooling, individual total RNA quantity and concentration were measured on a Bioanalyzer 2100 and with an RNA 6000 Nano LabChip Kit (Agilent, CA, USA) with RNA integrity number (RIN) > 8.0.

Article Title: Key Genes and Pathways Associated With Inner Ear Malformation in SOX10 p.R109W Mutation Pigs
Article Snippet: Paragraph title: RNA Isolation From Otic Tissue ... The quantity and purity of the collected total RNA were analyzed with a Bioanalyzer 2200 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA).

Article Title: The miRNA-Mediated Post-Transcriptional Regulation of Maize in Response to High Temperature
Article Snippet: Paragraph title: 4.3. RNA Isolation, sRNA Library Preparation and Sequencing ... The quality and purity of the total RNA was analyzed using Bioanalyzer 2100 and an RNA 6000 Nano LabChip Kit (Agilent, Santa Clara, CA, USA).

Article Title: High throughput deep sequencing reveals the important roles of microRNAs during sweetpotato storage at chilling temperature
Article Snippet: Paragraph title: Plant materials, sample collection and RNA isolation ... The quantity and purity of total RNA were analyzed with a Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA) with RIN number > 7.0.

Labeling:

Article Title: Lysozyme association with circulating RNA, extracellular vesicles, and chronic stress
Article Snippet: RNA quantity, purity, and integrity were assessed by a NanoDropTM 1000 spectrophotometer (Wilmington, DE) or by 2100 Bioanalyzer using the RNA 6000 Nano LabChip kit (Agilent Technologies, Santa Clara, CA). .. RNA quantity, purity, and integrity were assessed by a NanoDropTM 1000 spectrophotometer (Wilmington, DE) or by 2100 Bioanalyzer using the RNA 6000 Nano LabChip kit (Agilent Technologies, Santa Clara, CA).

Article Title: Global Gene-expression Analysis of the Response of Salmonella Enteritidis to Egg White Exposure Reveals Multiple Egg White-imposed Stress Responses
Article Snippet: For the microarray experiment, additional analysis for RNA integrity was performed using a RNA 6000 Nano LabChip kit (2100 Bioanalyzer, Agilent Technologies, Santa Clara, CA, USA) (Mueller et al., ). .. For the microarray experiment, additional analysis for RNA integrity was performed using a RNA 6000 Nano LabChip kit (2100 Bioanalyzer, Agilent Technologies, Santa Clara, CA, USA) (Mueller et al., ).

Purification:

Article Title: A large family of Dscam genes with tandemly arrayed 5′ cassettes in Chelicerata
Article Snippet: The total RNA quantity and purity were analysed using a Bioanalyser 2100 and RNA 6000 Nano LabChip Kit (Agilent, Santa Clara, CA, USA) with RNA integrity number > 7.0. .. For the RNA-seq experiment, ∼10 μg of total RNA was subjected to enrichment of the poly(A)-tailed mRNAs with poly(T) oligo-attached magnetic beads (Thermo Fisher Scientific, Waltham, MA, USA).

Article Title: The roles of TRIO and F-actin-binding protein in glioblastoma cells
Article Snippet: The total RNA quantity and purity were analysis of Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent Technologies, Inc., Santa Clara, CA, USA). .. Roughly 10 µg of total RNA was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen; Thermo Fisher Scientific, Inc.).

Article Title: Transcriptome Analysis of HepG2 Cells Expressing ORF3 from Swine Hepatitis E Virus to Determine the Effects of ORF3 on Host Cells
Article Snippet: The quantity and purity of the total RNA were analyzed using Bioanalyzer 2100 and the RNA 6000 Nano LabChip Kit (Agilent Technologies, Santa Clara, CA, USA). .. Next, 10 μ g of RNA from specific cell lines (ORF3-expressing HepG2 cells, EGFP (only)-expressing HepG2 cells, and HepG2 cells) was exposed to poly-T oligoattached magnetic beads (Invitrogen, Carlsbad, CA, USA) to isolate poly(A) mRNA.

Sequencing:

Article Title: Signature miRNAs in peripheral blood monocytes of patients with gastric or breast cancers
Article Snippet: Paragraph title: miRNA sequencing and sequence analysis ... The integrity of total RNAs was evaluated using an RNA 6000 Nano LabChip kit (Agilent Technologies, Palo Alto, CA, USA) with RIN number greater than 6.0.

Article Title: Integrated analysis of high-throughput sequencing data shows abscisic acid-responsive genes and miRNAs in strawberry receptacle fruit ripening
Article Snippet: Paragraph title: RNA extraction, library construction, and Illumina sequencing ... Before intrasample pooling, individual total RNA quantity and concentration were measured on a Bioanalyzer 2100 and with an RNA 6000 Nano LabChip Kit (Agilent, CA, USA) with RNA integrity number (RIN) > 8.0.

Article Title: The miRNA-Mediated Post-Transcriptional Regulation of Maize in Response to High Temperature
Article Snippet: Paragraph title: 4.3. RNA Isolation, sRNA Library Preparation and Sequencing ... The quality and purity of the total RNA was analyzed using Bioanalyzer 2100 and an RNA 6000 Nano LabChip Kit (Agilent, Santa Clara, CA, USA).

Article Title: High throughput deep sequencing reveals the important roles of microRNAs during sweetpotato storage at chilling temperature
Article Snippet: The quantity and purity of total RNA were analyzed with a Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA) with RIN number > 7.0. .. Samples with 1–5 weeks of treatments for ATS and LTS were used for measuring the contents of MDA, starch and sucrose, and the activities of POD and SOD.

Article Title: Transcriptome Analysis of HepG2 Cells Expressing ORF3 from Swine Hepatitis E Virus to Determine the Effects of ORF3 on Host Cells
Article Snippet: Paragraph title: 2.6. mRNA Library Construction and Sequencing ... The quantity and purity of the total RNA were analyzed using Bioanalyzer 2100 and the RNA 6000 Nano LabChip Kit (Agilent Technologies, Santa Clara, CA, USA).

Construct:

Article Title: Transcriptome Analysis of HepG2 Cells Expressing ORF3 from Swine Hepatitis E Virus to Determine the Effects of ORF3 on Host Cells
Article Snippet: The quantity and purity of the total RNA were analyzed using Bioanalyzer 2100 and the RNA 6000 Nano LabChip Kit (Agilent Technologies, Santa Clara, CA, USA). .. Next, 10 μ g of RNA from specific cell lines (ORF3-expressing HepG2 cells, EGFP (only)-expressing HepG2 cells, and HepG2 cells) was exposed to poly-T oligoattached magnetic beads (Invitrogen, Carlsbad, CA, USA) to isolate poly(A) mRNA.

Lysis:

Article Title: Global Gene-expression Analysis of the Response of Salmonella Enteritidis to Egg White Exposure Reveals Multiple Egg White-imposed Stress Responses
Article Snippet: After defrosting on ice, cells were disrupted by treatment with TE (10 mM Tris-HCl pH 7, 1 mM EDTA) buffer, containing 20 mg/mL lysozyme, for 30 min at 37°C followed by mechanical lysis with zirconium beads using a FastPrep-24 instrument (MP Biomedicals, Illkirch, France) with two cycles of 30 s at 30 Hz interspaced with 30 s cooling periods. .. For the microarray experiment, additional analysis for RNA integrity was performed using a RNA 6000 Nano LabChip kit (2100 Bioanalyzer, Agilent Technologies, Santa Clara, CA, USA) (Mueller et al., ).

cDNA Library Assay:

Article Title: A large family of Dscam genes with tandemly arrayed 5′ cassettes in Chelicerata
Article Snippet: The total RNA quantity and purity were analysed using a Bioanalyser 2100 and RNA 6000 Nano LabChip Kit (Agilent, Santa Clara, CA, USA) with RNA integrity number > 7.0. .. After purification, the mRNA was fragmented into small pieces using divalent cations under elevated temperature.

Article Title: The roles of TRIO and F-actin-binding protein in glioblastoma cells
Article Snippet: The total RNA quantity and purity were analysis of Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent Technologies, Inc., Santa Clara, CA, USA). .. Following purification, the mRNA is fragmented into small pieces using divalent cations under raised temperature.

Article Title: Integrated analysis of high-throughput sequencing data shows abscisic acid-responsive genes and miRNAs in strawberry receptacle fruit ripening
Article Snippet: Before intrasample pooling, individual total RNA quantity and concentration were measured on a Bioanalyzer 2100 and with an RNA 6000 Nano LabChip Kit (Agilent, CA, USA) with RNA integrity number (RIN) > 8.0. .. Before intrasample pooling, individual total RNA quantity and concentration were measured on a Bioanalyzer 2100 and with an RNA 6000 Nano LabChip Kit (Agilent, CA, USA) with RNA integrity number (RIN) > 8.0.

Article Title: Transcriptome Analysis Reveals Cotton (Gossypium hirsutum) Genes That Are Differentially Expressed in Cadmium Stress Tolerance
Article Snippet: Paragraph title: 4.3. RNA Extraction, cDNA Library Construction, and RNA-Seq ... Total RNA quantity and purity were measured with Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, Santa Clara, CA, USA) with RIN number > 7, followed by gel extraction with 1% agarose gel electrophoresis.

Article Title: Transcriptome Analysis of HepG2 Cells Expressing ORF3 from Swine Hepatitis E Virus to Determine the Effects of ORF3 on Host Cells
Article Snippet: The quantity and purity of the total RNA were analyzed using Bioanalyzer 2100 and the RNA 6000 Nano LabChip Kit (Agilent Technologies, Santa Clara, CA, USA). .. Next, 10 μ g of RNA from specific cell lines (ORF3-expressing HepG2 cells, EGFP (only)-expressing HepG2 cells, and HepG2 cells) was exposed to poly-T oligoattached magnetic beads (Invitrogen, Carlsbad, CA, USA) to isolate poly(A) mRNA.

Agarose Gel Electrophoresis:

Article Title: An improved method for isolation of RNA from bone
Article Snippet: RNA Quality and Yield RNA yield was determined by the absorbance at 260 nm (Table ) and using the Agilent RNA 6000 Nano LabChip Kit and the Agilent 2100 Bioanalyzer (Agilent Technologies). .. RNA Quality and Yield RNA yield was determined by the absorbance at 260 nm (Table ) and using the Agilent RNA 6000 Nano LabChip Kit and the Agilent 2100 Bioanalyzer (Agilent Technologies).

Article Title: Transcriptome Analysis Reveals Cotton (Gossypium hirsutum) Genes That Are Differentially Expressed in Cadmium Stress Tolerance
Article Snippet: The total RNA was extracted using Trizol reagent (Invitrogen, Carlsbad, CA, USA) following the manufacturer’s procedure. .. Total RNA quantity and purity were measured with Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, Santa Clara, CA, USA) with RIN number > 7, followed by gel extraction with 1% agarose gel electrophoresis. .. Then approximately 10 µg of total RNA was purified using poly-T oligo-attached magnetic beads cleaved into smaller fragments with fragmentation buffer.

Liquid Chromatography:

Article Title: Transcriptome Analysis of HepG2 Cells Expressing ORF3 from Swine Hepatitis E Virus to Determine the Effects of ORF3 on Host Cells
Article Snippet: The quantity and purity of the total RNA were analyzed using Bioanalyzer 2100 and the RNA 6000 Nano LabChip Kit (Agilent Technologies, Santa Clara, CA, USA). .. Following purification, the mRNA was fragmented into small pieces using fragmentation buffer and the cleaved RNA fragments were reverse-transcribed to construct a cDNA library using the mRNA-Seq sample preparation kit (Illumina, San Diego, CA, USA), according to the manufacturer's instructions.

RNA Extraction:

Article Title: Substantial Dysregulation of miRNA Passenger Strands Underlies the Vascular Response to Injury
Article Snippet: All the procedures for RNA extraction from the pig vein graft model and stent model are described in detail in [ , ], respectively. .. RNA integrity was assessed using the RNA 6000 Nano LabChip kit (Agilent Technologies, Santa Clara, CA, USA).

Article Title: Characterisation of the Whole Blood mRNA Transcriptome in Holstein-Friesian and Jersey Calves in Response to Gradual Weaning
Article Snippet: Paragraph title: RNA extraction from whole blood ... The quality of the extracted RNA was examined with the Agilent 2100 Bioanalyser (Agilent Technologies Ireland Ltd; Dublin, Ireland) using the RNA 6000 Nano LabChip kit (Agilent Technologies Ireland Ltd; Dublin, Ireland).

Article Title: Antisense RNA transcripts in the blood may be novel diagnostic markers for colorectal cancer
Article Snippet: Paragraph title: Total RNA extraction from blood cells ... The quantity of total RNA obtained was determined using a NanoDrop Spectrophotometer (NanoDrop Technologies; Thermo Fisher Scientific, Inc., Waltham, MA, USA) according to the 280/260 nm absorbance ratio, and RNA integrity was evaluated using an RNA 6000 Nano LabChip kit (Agilent Technologies, Inc., Santa Clara, CA, USA) and an Agilent 2100 Bioanalyzer (Agilent Technologies, Inc.).

Article Title: Integrated analysis of high-throughput sequencing data shows abscisic acid-responsive genes and miRNAs in strawberry receptacle fruit ripening
Article Snippet: Paragraph title: RNA extraction, library construction, and Illumina sequencing ... Before intrasample pooling, individual total RNA quantity and concentration were measured on a Bioanalyzer 2100 and with an RNA 6000 Nano LabChip Kit (Agilent, CA, USA) with RNA integrity number (RIN) > 8.0.

Article Title: Transcriptome Analysis Reveals Cotton (Gossypium hirsutum) Genes That Are Differentially Expressed in Cadmium Stress Tolerance
Article Snippet: Paragraph title: 4.3. RNA Extraction, cDNA Library Construction, and RNA-Seq ... Total RNA quantity and purity were measured with Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, Santa Clara, CA, USA) with RIN number > 7, followed by gel extraction with 1% agarose gel electrophoresis.

Article Title: Differential effects of Tat proteins derived from HIV-1 subtypes B and recombinant CRF02_AG on human brain microvascular endothelial cells: implications for blood–brain barrier dysfunction
Article Snippet: Paragraph title: Tat Treatment of Human Brain Microvascular Endothelial Cells and RNA Extraction ... The purity and quality of extracted RNA were further assessed using the RNA 6000 Nano LabChip Kit and Agilent-2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA, USA) according to the manufacturer's instructions.

Sample Prep:

Article Title: A large family of Dscam genes with tandemly arrayed 5′ cassettes in Chelicerata
Article Snippet: The total RNA quantity and purity were analysed using a Bioanalyser 2100 and RNA 6000 Nano LabChip Kit (Agilent, Santa Clara, CA, USA) with RNA integrity number > 7.0. .. After purification, the mRNA was fragmented into small pieces using divalent cations under elevated temperature.

Article Title: The roles of TRIO and F-actin-binding protein in glioblastoma cells
Article Snippet: The total RNA quantity and purity were analysis of Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent Technologies, Inc., Santa Clara, CA, USA). .. Following purification, the mRNA is fragmented into small pieces using divalent cations under raised temperature.

Article Title: Integrated analysis of high-throughput sequencing data shows abscisic acid-responsive genes and miRNAs in strawberry receptacle fruit ripening
Article Snippet: Before intrasample pooling, individual total RNA quantity and concentration were measured on a Bioanalyzer 2100 and with an RNA 6000 Nano LabChip Kit (Agilent, CA, USA) with RNA integrity number (RIN) > 8.0. .. After pooling, 14 RNA samples were prepared for cDNA library construction.

Article Title: Transcriptome Analysis Reveals Cotton (Gossypium hirsutum) Genes That Are Differentially Expressed in Cadmium Stress Tolerance
Article Snippet: Total RNA quantity and purity were measured with Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, Santa Clara, CA, USA) with RIN number > 7, followed by gel extraction with 1% agarose gel electrophoresis. .. Then the cleaved RNA fragments were transcribed to first-strand cDNA fragments using reverse transcriptase and a high concentration of random hexamer primer.

Article Title: Transcriptome Analysis of HepG2 Cells Expressing ORF3 from Swine Hepatitis E Virus to Determine the Effects of ORF3 on Host Cells
Article Snippet: The quantity and purity of the total RNA were analyzed using Bioanalyzer 2100 and the RNA 6000 Nano LabChip Kit (Agilent Technologies, Santa Clara, CA, USA). .. Next, 10 μ g of RNA from specific cell lines (ORF3-expressing HepG2 cells, EGFP (only)-expressing HepG2 cells, and HepG2 cells) was exposed to poly-T oligoattached magnetic beads (Invitrogen, Carlsbad, CA, USA) to isolate poly(A) mRNA.

Random Hexamer Labeling:

Article Title: Transcriptome Analysis Reveals Cotton (Gossypium hirsutum) Genes That Are Differentially Expressed in Cadmium Stress Tolerance
Article Snippet: Total RNA quantity and purity were measured with Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, Santa Clara, CA, USA) with RIN number > 7, followed by gel extraction with 1% agarose gel electrophoresis. .. Then approximately 10 µg of total RNA was purified using poly-T oligo-attached magnetic beads cleaved into smaller fragments with fragmentation buffer.

Spectrophotometry:

Article Title: Substantial Dysregulation of miRNA Passenger Strands Underlies the Vascular Response to Injury
Article Snippet: The RNAs were processed through miRNEasy Mini Kit (Qiagen) following the manufacturer’s instructions, treated with DNAse 1 (amplification grade; Sigma, St. Louis, MO, USA) in order to eliminate genomic DNA contamination and quantified using a NanoDrop ND-1000 Spectrophotometer (Nano-Drop Technologies, Wilmington, DE, USA). .. RNA integrity was assessed using the RNA 6000 Nano LabChip kit (Agilent Technologies, Santa Clara, CA, USA).

Article Title: Lysozyme association with circulating RNA, extracellular vesicles, and chronic stress
Article Snippet: 2.2 Peripheral whole blood was collected in PAXgene® RNA tubes and total RNA was isolated with PAXgene® Blood miRNA kit as per the manufacturer's instructions. .. RNA quantity, purity, and integrity were assessed by a NanoDropTM 1000 spectrophotometer (Wilmington, DE) or by 2100 Bioanalyzer using the RNA 6000 Nano LabChip kit (Agilent Technologies, Santa Clara, CA). .. All samples yielded high quality score (RIN > 9).

Article Title: Characterisation of the Whole Blood mRNA Transcriptome in Holstein-Friesian and Jersey Calves in Response to Gradual Weaning
Article Snippet: The quantity of the extracted RNA was determined by measuring the absorbance at 260 nm with a Nanodrop spectrophotometer (NanoDrop technologies; Wilmington, DE, USA). .. The quality of the extracted RNA was examined with the Agilent 2100 Bioanalyser (Agilent Technologies Ireland Ltd; Dublin, Ireland) using the RNA 6000 Nano LabChip kit (Agilent Technologies Ireland Ltd; Dublin, Ireland).

Article Title: Antisense RNA transcripts in the blood may be novel diagnostic markers for colorectal cancer
Article Snippet: Blood samples were collected from the patients using PAXgene Vacutainer tubes (BD Biosciences, Franklin Lakes, NJ, USA) and subjected to RNA isolation and extraction using a PAXgene Blood RNA Isolation kit (Qiagen, Inc., Valencia, CA, USA), following the manufacturer's instructions. .. The quantity of total RNA obtained was determined using a NanoDrop Spectrophotometer (NanoDrop Technologies; Thermo Fisher Scientific, Inc., Waltham, MA, USA) according to the 280/260 nm absorbance ratio, and RNA integrity was evaluated using an RNA 6000 Nano LabChip kit (Agilent Technologies, Inc., Santa Clara, CA, USA) and an Agilent 2100 Bioanalyzer (Agilent Technologies, Inc.). .. Cyanine 3 (Cy3)-labeled cDNA was synthesized from 10 µg total RNA extracted from the blood samples using a LabelStar Array kit (Qiagen, Inc.) with Cy3-dUTP (GE Healthcare Bio-Sciences, Pittsburgh, PA, USA) and random nonamer primers.

Article Title: Differential effects of Tat proteins derived from HIV-1 subtypes B and recombinant CRF02_AG on human brain microvascular endothelial cells: implications for blood–brain barrier dysfunction
Article Snippet: RNA yield and quality were checked using a NanoDrop spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA) and for all samples, the 260/280 absorbance ratio was ⩾2. .. The purity and quality of extracted RNA were further assessed using the RNA 6000 Nano LabChip Kit and Agilent-2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA, USA) according to the manufacturer's instructions.

Produced:

Article Title: An improved method for isolation of RNA from bone
Article Snippet: RNA Quality and Yield RNA yield was determined by the absorbance at 260 nm (Table ) and using the Agilent RNA 6000 Nano LabChip Kit and the Agilent 2100 Bioanalyzer (Agilent Technologies). .. In addition to assaying the RNA for intact 28S and 18S rRNA (Figure ) using agarose gel electrophoresis, we determined the quality of the isolated RNA using the Agilent 2100 Bioanalyzer.

Concentration Assay:

Article Title: Integrated analysis of high-throughput sequencing data shows abscisic acid-responsive genes and miRNAs in strawberry receptacle fruit ripening
Article Snippet: For each sample, RNA was extracted separately from ten randomly selected individual fruit receptacles. .. Before intrasample pooling, individual total RNA quantity and concentration were measured on a Bioanalyzer 2100 and with an RNA 6000 Nano LabChip Kit (Agilent, CA, USA) with RNA integrity number (RIN) > 8.0. .. The intrapooled sample was set as one biological replication, and two replications were prepared for the deep-sequencing analysis.

Article Title: Transcriptome Analysis Reveals Cotton (Gossypium hirsutum) Genes That Are Differentially Expressed in Cadmium Stress Tolerance
Article Snippet: Total RNA quantity and purity were measured with Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, Santa Clara, CA, USA) with RIN number > 7, followed by gel extraction with 1% agarose gel electrophoresis. .. Then approximately 10 µg of total RNA was purified using poly-T oligo-attached magnetic beads cleaved into smaller fragments with fragmentation buffer.

High Throughput Screening Assay:

Article Title: Signature miRNAs in peripheral blood monocytes of patients with gastric or breast cancers
Article Snippet: The integrity of total RNAs was evaluated using an RNA 6000 Nano LabChip kit (Agilent Technologies, Palo Alto, CA, USA) with RIN number greater than 6.0. .. Then, RNAs were used to construct small RNA libraries and sequenced on an Illumina HiSeq 2500/2000 platform (Novogene Company, Beijing, China).

Gel Extraction:

Article Title: Transcriptome Analysis Reveals Cotton (Gossypium hirsutum) Genes That Are Differentially Expressed in Cadmium Stress Tolerance
Article Snippet: The total RNA was extracted using Trizol reagent (Invitrogen, Carlsbad, CA, USA) following the manufacturer’s procedure. .. Total RNA quantity and purity were measured with Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, Santa Clara, CA, USA) with RIN number > 7, followed by gel extraction with 1% agarose gel electrophoresis. .. Then approximately 10 µg of total RNA was purified using poly-T oligo-attached magnetic beads cleaved into smaller fragments with fragmentation buffer.

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Agilent technologies rna 6000 nano labchip kit
    Extracting High Quality RNA from Bone in a Single Step . (A) Isolated liver RNA was incubated with RNase free beads (lane 1) or untreated beads (lane 2) provided by the manufacturer (Next Advance) for four hours before analyzing the 18S and 28S rRNA bands using agarose gel chromatography. Subsequent experiments with the Bullet Blender were carried out using untreated beads. (B) Bone RNA was homogenized in near freezing conditions using the Bullet Blender centrifuge (lanes 2-4) or a Polytron (lanes 5-6). The results are compared to bone RNA isolated using standard homogenization conditions (lane 1) and intact liver RNA that was previously isolated (control). (C) The RNA Integrity Number (RIN) for RNA homogenized in near freezing conditions was determined using the Agilent RNA 6000 <t>Nano</t> <t>LabChip</t> Kit and the Agilent 2100 Bioanalyzer. RNA with a RIN = 7 or greater is suitable for microarray analysis of gene expression. (D) The maximum RIN for the two-step approach (lane 2) is compared to the maximum RIN obtained in the one-step approach (lane 3) and each RIN is compared to the RIN of the degraded RNA sample (lane 1) shown in the agarose gel in lane 1, Figure 2B. (E) The electropherograms associated with the samples shown in (D).
    Rna 6000 Nano Labchip Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 123 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rna 6000 nano labchip kit/product/Agilent technologies
    Average 99 stars, based on 123 article reviews
    Price from $9.99 to $1999.99
    rna 6000 nano labchip kit - by Bioz Stars, 2019-09
    99/100 stars
      Buy from Supplier

    Image Search Results


    Extracting High Quality RNA from Bone in a Single Step . (A) Isolated liver RNA was incubated with RNase free beads (lane 1) or untreated beads (lane 2) provided by the manufacturer (Next Advance) for four hours before analyzing the 18S and 28S rRNA bands using agarose gel chromatography. Subsequent experiments with the Bullet Blender were carried out using untreated beads. (B) Bone RNA was homogenized in near freezing conditions using the Bullet Blender centrifuge (lanes 2-4) or a Polytron (lanes 5-6). The results are compared to bone RNA isolated using standard homogenization conditions (lane 1) and intact liver RNA that was previously isolated (control). (C) The RNA Integrity Number (RIN) for RNA homogenized in near freezing conditions was determined using the Agilent RNA 6000 Nano LabChip Kit and the Agilent 2100 Bioanalyzer. RNA with a RIN = 7 or greater is suitable for microarray analysis of gene expression. (D) The maximum RIN for the two-step approach (lane 2) is compared to the maximum RIN obtained in the one-step approach (lane 3) and each RIN is compared to the RIN of the degraded RNA sample (lane 1) shown in the agarose gel in lane 1, Figure 2B. (E) The electropherograms associated with the samples shown in (D).

    Journal: BMC Biotechnology

    Article Title: An improved method for isolation of RNA from bone

    doi: 10.1186/1472-6750-12-5

    Figure Lengend Snippet: Extracting High Quality RNA from Bone in a Single Step . (A) Isolated liver RNA was incubated with RNase free beads (lane 1) or untreated beads (lane 2) provided by the manufacturer (Next Advance) for four hours before analyzing the 18S and 28S rRNA bands using agarose gel chromatography. Subsequent experiments with the Bullet Blender were carried out using untreated beads. (B) Bone RNA was homogenized in near freezing conditions using the Bullet Blender centrifuge (lanes 2-4) or a Polytron (lanes 5-6). The results are compared to bone RNA isolated using standard homogenization conditions (lane 1) and intact liver RNA that was previously isolated (control). (C) The RNA Integrity Number (RIN) for RNA homogenized in near freezing conditions was determined using the Agilent RNA 6000 Nano LabChip Kit and the Agilent 2100 Bioanalyzer. RNA with a RIN = 7 or greater is suitable for microarray analysis of gene expression. (D) The maximum RIN for the two-step approach (lane 2) is compared to the maximum RIN obtained in the one-step approach (lane 3) and each RIN is compared to the RIN of the degraded RNA sample (lane 1) shown in the agarose gel in lane 1, Figure 2B. (E) The electropherograms associated with the samples shown in (D).

    Article Snippet: RNA yield was determined by the absorbance at 260 nm (Table ) and using the Agilent RNA 6000 Nano LabChip Kit and the Agilent 2100 Bioanalyzer (Agilent Technologies).

    Techniques: Isolation, Incubation, Agarose Gel Electrophoresis, Chromatography, Homogenization, Microarray, Expressing

    Analysis of IL-6 mRNA levels within synovial tissue from rheumatoid arthritis (RA) as compared with that from osteoarthritis (OA) patients. Upper panels: quality control of total RNA preparations. Aliquots (300 ng) of total RNA extracted from synovial tissue from RA and OA patients were plotted on a RNA 6000 Nano-LabChip. Quality of RNA was scanned using a 2100 bioanalyzer. RNA gel electropherograms show the presence of 28S and 18S ribosomal units, indicating intact RNA of the investigated samples. Lower panels: differential IL-6 mRNA levels were determined by semiquantitative reverse transcription polymerase chain reaction (PCR). The figure shows a representative analysis of eight cDNA samples derived from patients with RA and of eight cDNA samples from patients with OA. cDNA samples were adjusted to equal glyceraldehyde-3-phosphate dehydrogenase (G3PDH) levels, performed by competitive PCR using an internal standard (see Materials and methods). Numbered lanes correspond to individual patients within Table 1 .

    Journal: Arthritis Research & Therapy

    Article Title: High CXCR3 expression in synovial mast cells associated with CXCL9 and CXCL10 expression in inflammatory synovial tissues of patients with rheumatoid arthritis

    doi:

    Figure Lengend Snippet: Analysis of IL-6 mRNA levels within synovial tissue from rheumatoid arthritis (RA) as compared with that from osteoarthritis (OA) patients. Upper panels: quality control of total RNA preparations. Aliquots (300 ng) of total RNA extracted from synovial tissue from RA and OA patients were plotted on a RNA 6000 Nano-LabChip. Quality of RNA was scanned using a 2100 bioanalyzer. RNA gel electropherograms show the presence of 28S and 18S ribosomal units, indicating intact RNA of the investigated samples. Lower panels: differential IL-6 mRNA levels were determined by semiquantitative reverse transcription polymerase chain reaction (PCR). The figure shows a representative analysis of eight cDNA samples derived from patients with RA and of eight cDNA samples from patients with OA. cDNA samples were adjusted to equal glyceraldehyde-3-phosphate dehydrogenase (G3PDH) levels, performed by competitive PCR using an internal standard (see Materials and methods). Numbered lanes correspond to individual patients within Table 1 .

    Article Snippet: Quality of all total RNA samples was controlled by a 2100 bioanalyzer according to a RNA 6000 Nano-LabChip Kit procedure (Agilent Technologies, Palo Alto, CA, USA), using 0.3 μg of each total RNA. cDNA was synthesized from 1 μg total RNA in a 20 μl reaction using 200 U Superscript™ II reverse transcriptase (Life Technologies), 500 μmol/l of each deoxynucleotide, 5 mmol/l DTT and 0.5 μg of oligo(dT)15 (Invitek, Berlin, Germany).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Polymerase Chain Reaction, Derivative Assay