Structured Review

Agilent technologies rna 6000 nano kit
RNA integrity, protein levels and degradation, and cells size of stationary phase cells ( A–C, E–F ) Cell cultures at early stationary phase (t=6 h) were treated with 1mM KCN or transferred to an anaerobic chamber. At t=24 h, cells were pelleted for RNA, protein, and microscope analyses. For controls, untreated overnight cultures (t=24 h) and early stationary phase cultures (t=6 h) were used. ( A–B ) RNA quality was determined with a bioanalyzer using an <t>RNA</t> 6000 <t>Nano</t> kit. The degradation of rRNA was assessed with RNA integrity values which range from 10 (intact) to 1 (totally degraded). (C) Cells were sonicated and the protein content in the supernatant was determined with Bradford assays. (D) Before KCN treatment at t=6 h, the inducer for gfp expression was removed in the cultures with the cells carrying pQE-80L gfp-ssrA . After the KCN treatment, GFP levels were measured. Background fluorescence was determined using cells with empty vectors. (E–F) Phase contrast images of fixed cells were taken using a microscope, and cell size (fold change relative to 24 h untreated overnight cultures) were determined with ImageJ. (G–H) KCN treatment was performed at t=9 h. At t=24 h, microscope images were taken, and ampicillin and ofloxacin persister levels were determined. * signifies significant differences for comparisons to control groups (p-value
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Images

1) Product Images from "Inhibition of stationary phase respiration impairs persister formation in E. coli"

Article Title: Inhibition of stationary phase respiration impairs persister formation in E. coli

Journal: Nature communications

doi: 10.1038/ncomms8983

RNA integrity, protein levels and degradation, and cells size of stationary phase cells ( A–C, E–F ) Cell cultures at early stationary phase (t=6 h) were treated with 1mM KCN or transferred to an anaerobic chamber. At t=24 h, cells were pelleted for RNA, protein, and microscope analyses. For controls, untreated overnight cultures (t=24 h) and early stationary phase cultures (t=6 h) were used. ( A–B ) RNA quality was determined with a bioanalyzer using an RNA 6000 Nano kit. The degradation of rRNA was assessed with RNA integrity values which range from 10 (intact) to 1 (totally degraded). (C) Cells were sonicated and the protein content in the supernatant was determined with Bradford assays. (D) Before KCN treatment at t=6 h, the inducer for gfp expression was removed in the cultures with the cells carrying pQE-80L gfp-ssrA . After the KCN treatment, GFP levels were measured. Background fluorescence was determined using cells with empty vectors. (E–F) Phase contrast images of fixed cells were taken using a microscope, and cell size (fold change relative to 24 h untreated overnight cultures) were determined with ImageJ. (G–H) KCN treatment was performed at t=9 h. At t=24 h, microscope images were taken, and ampicillin and ofloxacin persister levels were determined. * signifies significant differences for comparisons to control groups (p-value
Figure Legend Snippet: RNA integrity, protein levels and degradation, and cells size of stationary phase cells ( A–C, E–F ) Cell cultures at early stationary phase (t=6 h) were treated with 1mM KCN or transferred to an anaerobic chamber. At t=24 h, cells were pelleted for RNA, protein, and microscope analyses. For controls, untreated overnight cultures (t=24 h) and early stationary phase cultures (t=6 h) were used. ( A–B ) RNA quality was determined with a bioanalyzer using an RNA 6000 Nano kit. The degradation of rRNA was assessed with RNA integrity values which range from 10 (intact) to 1 (totally degraded). (C) Cells were sonicated and the protein content in the supernatant was determined with Bradford assays. (D) Before KCN treatment at t=6 h, the inducer for gfp expression was removed in the cultures with the cells carrying pQE-80L gfp-ssrA . After the KCN treatment, GFP levels were measured. Background fluorescence was determined using cells with empty vectors. (E–F) Phase contrast images of fixed cells were taken using a microscope, and cell size (fold change relative to 24 h untreated overnight cultures) were determined with ImageJ. (G–H) KCN treatment was performed at t=9 h. At t=24 h, microscope images were taken, and ampicillin and ofloxacin persister levels were determined. * signifies significant differences for comparisons to control groups (p-value

Techniques Used: Microscopy, Sonication, Expressing, Fluorescence, Significance Assay

2) Product Images from "RNA Catabolites Contribute to the Nitrogen Pool and Support Growth Recovery of Wheat"

Article Title: RNA Catabolites Contribute to the Nitrogen Pool and Support Growth Recovery of Wheat

Journal: Frontiers in Plant Science

doi: 10.3389/fpls.2018.01539

The quantity and quality of nucleic acids of wheat shoots. Total DNA (A) and RNA (B) yields extracted from Mace and RAC875 whole shoots ( Experiment 1 ) as determined by fluorescence assays (Quant-it dsDNA broad range kit and Quant-iT RNA Assay Kit, Life Technologies, United States). (C,D) RNA quality assessment via RNA smear analysis using an RNA 6000 Nano Kit (Agilent, United States) and the Agilent 2100 Bioanalyzer. The chromatograms (C,D) show the fluorescence ( y -axis fluorescent units, FU) of rRNA species over time ( x -axis, seconds, s) with the faster migrating 25S peak at 45.8 s and the slower 18S rRNA peak at 41.35 s. Representative image of a +N treated RAC875 shoot sample (C) and a -N treated RAC875 shoot sample (D) .
Figure Legend Snippet: The quantity and quality of nucleic acids of wheat shoots. Total DNA (A) and RNA (B) yields extracted from Mace and RAC875 whole shoots ( Experiment 1 ) as determined by fluorescence assays (Quant-it dsDNA broad range kit and Quant-iT RNA Assay Kit, Life Technologies, United States). (C,D) RNA quality assessment via RNA smear analysis using an RNA 6000 Nano Kit (Agilent, United States) and the Agilent 2100 Bioanalyzer. The chromatograms (C,D) show the fluorescence ( y -axis fluorescent units, FU) of rRNA species over time ( x -axis, seconds, s) with the faster migrating 25S peak at 45.8 s and the slower 18S rRNA peak at 41.35 s. Representative image of a +N treated RAC875 shoot sample (C) and a -N treated RAC875 shoot sample (D) .

Techniques Used: Fluorescence

3) Product Images from "RNA Catabolites Contribute to the Nitrogen Pool and Support Growth Recovery of Wheat"

Article Title: RNA Catabolites Contribute to the Nitrogen Pool and Support Growth Recovery of Wheat

Journal: Frontiers in Plant Science

doi: 10.3389/fpls.2018.01539

The quantity and quality of nucleic acids of wheat shoots. Total DNA (A) and RNA (B) yields extracted from Mace and RAC875 whole shoots ( Experiment 1 ) as determined by fluorescence assays (Quant-it dsDNA broad range kit and Quant-iT RNA Assay Kit, Life Technologies, United States). (C,D) RNA quality assessment via RNA smear analysis using an RNA 6000 Nano Kit (Agilent, United States) and the Agilent 2100 Bioanalyzer. The chromatograms (C,D) show the fluorescence ( y -axis fluorescent units, FU) of rRNA species over time ( x -axis, seconds, s) with the faster migrating 25S peak at 45.8 s and the slower 18S rRNA peak at 41.35 s. Representative image of a +N treated RAC875 shoot sample (C) and a -N treated RAC875 shoot sample (D) .
Figure Legend Snippet: The quantity and quality of nucleic acids of wheat shoots. Total DNA (A) and RNA (B) yields extracted from Mace and RAC875 whole shoots ( Experiment 1 ) as determined by fluorescence assays (Quant-it dsDNA broad range kit and Quant-iT RNA Assay Kit, Life Technologies, United States). (C,D) RNA quality assessment via RNA smear analysis using an RNA 6000 Nano Kit (Agilent, United States) and the Agilent 2100 Bioanalyzer. The chromatograms (C,D) show the fluorescence ( y -axis fluorescent units, FU) of rRNA species over time ( x -axis, seconds, s) with the faster migrating 25S peak at 45.8 s and the slower 18S rRNA peak at 41.35 s. Representative image of a +N treated RAC875 shoot sample (C) and a -N treated RAC875 shoot sample (D) .

Techniques Used: Fluorescence

4) Product Images from "The SmAP1/2 proteins of the crenarchaeon Sulfolobus solfataricus interact with the exosome and stimulate A-rich tailing of transcripts"

Article Title: The SmAP1/2 proteins of the crenarchaeon Sulfolobus solfataricus interact with the exosome and stimulate A-rich tailing of transcripts

Journal: Nucleic Acids Research

doi: 10.1093/nar/gkx437

Elevated levels of the SmAPs increase the abundance of RNAs with A-rich tails. ( A ) Total RNA was isolated from strains PH1-16(pMJ05-SmAP1-His) and PH1-16(pMJO5-SmAP2-His) grown either in the presence of sucrose (−, non-induced) (lanes 1 and 3) or arabinose (+, induced) (lanes 2 and 4). Equal amounts of total RNA were used to isolate adenylated RNA with the Oligotex™ kit. Two microliter of each eluate was analyzed using with the Agilent 2100 Bioanalyzer (Agilent Technologies) and the RNA 6000 Nano Kit (Agilent Technologies). ( B ) The sequence composition of the A-rich tails obtained after over-production of SmAP1 (top) and SmAP2 (bottom) was determined using WebLogo ( 51 ). Only 3΄ ends of the adaptor clipped reads, which do not map to the reference genome and which showed an overhang of longer than 15 nt were used for the analyzes. Only sites present in both replicas that are supported by at least five independent reads were analyzed. ( C ) Functional categorization of tailed RNAs. Functions, which are significant enriched (Fisher's exact test; α = 0.05) are marked with an asterisk. Genes are annotated according to ( 54 ).
Figure Legend Snippet: Elevated levels of the SmAPs increase the abundance of RNAs with A-rich tails. ( A ) Total RNA was isolated from strains PH1-16(pMJ05-SmAP1-His) and PH1-16(pMJO5-SmAP2-His) grown either in the presence of sucrose (−, non-induced) (lanes 1 and 3) or arabinose (+, induced) (lanes 2 and 4). Equal amounts of total RNA were used to isolate adenylated RNA with the Oligotex™ kit. Two microliter of each eluate was analyzed using with the Agilent 2100 Bioanalyzer (Agilent Technologies) and the RNA 6000 Nano Kit (Agilent Technologies). ( B ) The sequence composition of the A-rich tails obtained after over-production of SmAP1 (top) and SmAP2 (bottom) was determined using WebLogo ( 51 ). Only 3΄ ends of the adaptor clipped reads, which do not map to the reference genome and which showed an overhang of longer than 15 nt were used for the analyzes. Only sites present in both replicas that are supported by at least five independent reads were analyzed. ( C ) Functional categorization of tailed RNAs. Functions, which are significant enriched (Fisher's exact test; α = 0.05) are marked with an asterisk. Genes are annotated according to ( 54 ).

Techniques Used: Isolation, Sequencing, Functional Assay

5) Product Images from "Blue Light Acts as a Double-Edged Sword in Regulating Sexual Development of Hypocrea jecorina (Trichoderma reesei)"

Article Title: Blue Light Acts as a Double-Edged Sword in Regulating Sexual Development of Hypocrea jecorina (Trichoderma reesei)

Journal: PLoS ONE

doi: 10.1371/journal.pone.0044969

Transcription levels of putative genes involved in conidiation and sexual potency. Total RNAs were extracted from 8 different experimental conditions as indicated. The quality of extracted RNA samples was further analyzed with the RNA 6000 Nano kit by Agilent 2100 Bioanalyzer (see Materials and Methods). ( A ) Northern blots analysis of act1 (actin) and env1 transcription. The denaturing RNA agarose gel was stained with ethidium bromide, the 18S rRNA and 28S rRNA bands were clearly visible in the intact RNA samples. ( B–H ) qRT-PCR. Relative transcript abundance of representative genes in sexually potent and impotent conditions. Data were given as relative quantitative (RQ) values to one of the eight conditions as indicated. The transcripts of the ribosome protein gene rpl6e were used for normalization of the qRT-PCR data [43] .
Figure Legend Snippet: Transcription levels of putative genes involved in conidiation and sexual potency. Total RNAs were extracted from 8 different experimental conditions as indicated. The quality of extracted RNA samples was further analyzed with the RNA 6000 Nano kit by Agilent 2100 Bioanalyzer (see Materials and Methods). ( A ) Northern blots analysis of act1 (actin) and env1 transcription. The denaturing RNA agarose gel was stained with ethidium bromide, the 18S rRNA and 28S rRNA bands were clearly visible in the intact RNA samples. ( B–H ) qRT-PCR. Relative transcript abundance of representative genes in sexually potent and impotent conditions. Data were given as relative quantitative (RQ) values to one of the eight conditions as indicated. The transcripts of the ribosome protein gene rpl6e were used for normalization of the qRT-PCR data [43] .

Techniques Used: Northern Blot, Agarose Gel Electrophoresis, Staining, Quantitative RT-PCR

6) Product Images from "Sequence-Specific Cleavage of Small-Subunit (SSU) rRNA with Oligonucleotides and RNase H: a Rapid and Simple Approach to SSU rRNA-Based Quantitative Detection of Microorganisms"

Article Title: Sequence-Specific Cleavage of Small-Subunit (SSU) rRNA with Oligonucleotides and RNase H: a Rapid and Simple Approach to SSU rRNA-Based Quantitative Detection of Microorganisms

Journal: Applied and Environmental Microbiology

doi: 10.1128/AEM.70.6.3650-3663.2004

Effect of oligonucleotide type (G+C% and nucleotide length) on the 16S rRNA cleavage reaction. (A) Electropherogram of E. coli RNA digested with the 907-16 probe at 41°C, as resolved by an Agilent 2100 bioanalyzer with an RNA 6000 nano kit. Numbers with arrows indicate approximate estimates of the molecular weight of each peak (unit, nt). A gel-like image of the electropherogram is also shown in the graph; lane 1, RNA 6000 ladder marker (TaKaRa); lane 2, digested E. coli RNA fragments. (B) Temperature dependence of the rRNA cleavage reaction with the 907 probes. Percentages of cleaved 16S rRNA in the total 16S rRNA were directly estimated based on the peak areas of intact and cleaved 16S rRNA fragments in the electro- pherograms, and the percentages were plotted with the hybridization and digestion temperatures at which the respective reactions were performed. Error bars indicate the standard deviation of duplicate determinations. (C) Temperature dependence of the rRNA cleavage reaction with the 530 probes. Percentages of cleaved 16S rRNA in the total 16S rRNA were calculated in the same manner used for the graph in panel B and were plotted along with the hybridization and digestion temperatures used. Error bars indicate the standard deviation of duplicate determinations.
Figure Legend Snippet: Effect of oligonucleotide type (G+C% and nucleotide length) on the 16S rRNA cleavage reaction. (A) Electropherogram of E. coli RNA digested with the 907-16 probe at 41°C, as resolved by an Agilent 2100 bioanalyzer with an RNA 6000 nano kit. Numbers with arrows indicate approximate estimates of the molecular weight of each peak (unit, nt). A gel-like image of the electropherogram is also shown in the graph; lane 1, RNA 6000 ladder marker (TaKaRa); lane 2, digested E. coli RNA fragments. (B) Temperature dependence of the rRNA cleavage reaction with the 907 probes. Percentages of cleaved 16S rRNA in the total 16S rRNA were directly estimated based on the peak areas of intact and cleaved 16S rRNA fragments in the electro- pherograms, and the percentages were plotted with the hybridization and digestion temperatures at which the respective reactions were performed. Error bars indicate the standard deviation of duplicate determinations. (C) Temperature dependence of the rRNA cleavage reaction with the 530 probes. Percentages of cleaved 16S rRNA in the total 16S rRNA were calculated in the same manner used for the graph in panel B and were plotted along with the hybridization and digestion temperatures used. Error bars indicate the standard deviation of duplicate determinations.

Techniques Used: Molecular Weight, Marker, Hybridization, Standard Deviation

7) Product Images from "Microparticle conferred microRNA profiles - implications in the transfer and dominance of cancer traits"

Article Title: Microparticle conferred microRNA profiles - implications in the transfer and dominance of cancer traits

Journal: Molecular Cancer

doi: 10.1186/1476-4598-11-37

RNA integrity of samples. RNA derived from ( A ) the drug sensitive-recipient cell (CEM), ( B ) drug-resistant VLB 100 cells, ( C ) their isolated MPs (VLBMP) and ( D ) the drug sensitive-recipient cells after MP transfer (CEM + VLBMP) was analysed using Agilent RNA 6000 Nano kit by Agilent 2100 Bioanalyzer. The RIN value of the samples ranged between 6.2-9.2. Data is representative of a typical experiment
Figure Legend Snippet: RNA integrity of samples. RNA derived from ( A ) the drug sensitive-recipient cell (CEM), ( B ) drug-resistant VLB 100 cells, ( C ) their isolated MPs (VLBMP) and ( D ) the drug sensitive-recipient cells after MP transfer (CEM + VLBMP) was analysed using Agilent RNA 6000 Nano kit by Agilent 2100 Bioanalyzer. The RIN value of the samples ranged between 6.2-9.2. Data is representative of a typical experiment

Techniques Used: Derivative Assay, Isolation

Related Articles

Centrifugation:

Article Title: Blue Light Acts as a Double-Edged Sword in Regulating Sexual Development of Hypocrea jecorina (Trichoderma reesei)
Article Snippet: After centrifugation, aqueous phase was carefully transferred to a new microcentrifuge tube and mixed with equal volume of 100% ethanol to precipitate RNA. .. The quality of extracted RNA was further analyzed with the RNA 6000 Nano kit by Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA).

Article Title: Anxiety Associated Increased CpG Methylation in the Promoter of Asb1: A Translational Approach Evidenced by Epidemiological and Clinical Studies and a Murine Model
Article Snippet: RNA sample quality was qualitatively analyzed using capillary gel electrophoresis chip (RNA 6000 Nano Kit; Agilent, Boeblingen, Germany). .. Plasma was isolated following centrifugation at 6000 g at 4 °C for 15 min. A commercially available radioimmunoassay kit with 125 I-labeled anti-corticosterone antibody was used to determine plasma corticosterone levels (MP Biomedicals, Solon, OH, USA; sensitivity 12.5 ng/ml).

Amplification:

Article Title: Cloning and Variation of Ground State Intestinal Stem Cells
Article Snippet: Expression microarray and bioinformatics Total RNAs obtained from immature colonies and ALI differentiated structure were used for microarray preparation with WT Pico RNA Amplification System V2 for amplification of DNA and Encore Biotin Module for fragmentation and biotin labeling (NuGEN Technologies, USA). .. RNA quality (RNA integrity number, RIN) was measured by analysis using an Agilent 2100 Bioanalyzer and Agilent RNA 6000 Nano Kit (Agilent Technologies, USA).

Article Title: Anxiety Associated Increased CpG Methylation in the Promoter of Asb1: A Translational Approach Evidenced by Epidemiological and Clinical Studies and a Murine Model
Article Snippet: RNA sample quality was qualitatively analyzed using capillary gel electrophoresis chip (RNA 6000 Nano Kit; Agilent, Boeblingen, Germany). .. RNA samples of sufficient quality (RIN⩾7.5) were then processed with the Illumina TotalPrep-96 RNA Amplification Kit (Ambion, Austin, TX, USA) and hybridized onto MouseWG-6 v2 Expression BeadChips (Illumina, San Diego, CA, USA).

Synthesized:

Article Title: Blue Light Acts as a Double-Edged Sword in Regulating Sexual Development of Hypocrea jecorina (Trichoderma reesei)
Article Snippet: The quality of extracted RNA was further analyzed with the RNA 6000 Nano kit by Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA). .. For Northern blot analysis, DNA probes were synthesized by using a Nick Translation kit (GE Healthcare, United Kingdom).

Nick Translation:

Article Title: Blue Light Acts as a Double-Edged Sword in Regulating Sexual Development of Hypocrea jecorina (Trichoderma reesei)
Article Snippet: The quality of extracted RNA was further analyzed with the RNA 6000 Nano kit by Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA). .. For Northern blot analysis, DNA probes were synthesized by using a Nick Translation kit (GE Healthcare, United Kingdom).

Quantitative RT-PCR:

Article Title: Blue Light Acts as a Double-Edged Sword in Regulating Sexual Development of Hypocrea jecorina (Trichoderma reesei)
Article Snippet: Paragraph title: Quantification of Gene Expression by Northern Blot and qRT-PCR ... The quality of extracted RNA was further analyzed with the RNA 6000 Nano kit by Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA).

Electrophoresis:

Article Title: Sequence-Specific Cleavage of Small-Subunit (SSU) rRNA with Oligonucleotides and RNase H: a Rapid and Simple Approach to SSU rRNA-Based Quantitative Detection of Microorganisms
Article Snippet: Paragraph title: Electrophoresis of RNA fragments. ... The resultant RNA fragments were electrophoresed by either 1.5% agarose gels (Nusieve 3:1 agarose; BioWhittaker Molecular Applications) or the Agilent 2100 bioanalyzer with the RNA 6000 nano kit (Agilent).

Microarray:

Article Title: Genetic variability in the regulation of gene expression in ten regions of the human brain
Article Snippet: The quality of total RNA was evaluated by the 2100 Bioanalyzer (Agilent) and RNA 6000 Nano Kit (Agilent). .. All subsequent sample processing was performed on 96-well plates in the microarray facility (AROS Biotechnology, Denmark) within a specified timeframe.

Article Title: Cloning and Variation of Ground State Intestinal Stem Cells
Article Snippet: Paragraph title: Expression microarray and bioinformatics ... RNA quality (RNA integrity number, RIN) was measured by analysis using an Agilent 2100 Bioanalyzer and Agilent RNA 6000 Nano Kit (Agilent Technologies, USA).

Article Title: Cloning and Variation of Ground State Intestinal Stem Cells
Article Snippet: RNA quality (RNA integrity number, RIN) was measured by analysis Agilent 2100 Bioanalyzer and Agilent RNA 6000 Nano Kit (Agilent Technologies, USA). .. RNAs having a RIN > 8 were used for microarray analysis.

Article Title: Microparticle conferred microRNA profiles - implications in the transfer and dominance of cancer traits
Article Snippet: Analysis of RNA integrity and Affymetrix miRNA Arrays Total RNA integrity was analysed using the Agilent RNA 6000 Nano kit (Agilent Technologies Inc., Santa Clara, CA) and the result was analysed by Agilent 2100 Bioanalyzer (Agilent Technologies Inc.) as per the manufacturer’s recommendations; the RNA integrity number (RIN) of 10 represents the highest RNA integrity with minimal degradation and score of 1 is the lowest integrity [ ]. .. Nanodrop-1000 spectrophotometer (Nanodrop technologies, DE, USA) was used for the quantification of RNA and 500 ng of RNA from each sample was used for miRNA microarray analysis.

Expressing:

Article Title: Blue Light Acts as a Double-Edged Sword in Regulating Sexual Development of Hypocrea jecorina (Trichoderma reesei)
Article Snippet: Paragraph title: Quantification of Gene Expression by Northern Blot and qRT-PCR ... The quality of extracted RNA was further analyzed with the RNA 6000 Nano kit by Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA).

Article Title: Genetic variability in the regulation of gene expression in ten regions of the human brain
Article Snippet: The quality of total RNA was evaluated by the 2100 Bioanalyzer (Agilent) and RNA 6000 Nano Kit (Agilent). .. Processing with the Ambion WT Expression Kit and Affymetrix GeneChip Whole Transcript Sense Target Labeling Assay and hybridization to the Affymetrix Exon 1.0 ST arrays were performed in accordance with the manufacturers’ protocols.

Article Title: Rare coding variants in Phospholipase D3 (PLD3) confer risk for Alzheimer's disease
Article Snippet: .. The quality of total RNA was evaluated by the 2100 Bioanalyzer (Agilent, UK) and RNA 6000 Nano Kit (Agilent, UK) before processing with the Ambion® WT Expression Kit and Affymetrix GeneChip Whole Transcript Sense Target Labeling Assay and hybridization to the Affymetrix Exon 1.0 ST. All arrays were pre-processed using Robust Multi-array Average using Partek Genomics Suite v6.6 (Partek Incorporated, USA). .. The resulting expression data was corrected for individual effects (within which are nested postmortem interval, brain pH, sex, age at death and cause of death) and experimental batch effects (date of hybridization).

Article Title: Cloning and Variation of Ground State Intestinal Stem Cells
Article Snippet: Paragraph title: Expression microarray and bioinformatics ... RNA quality (RNA integrity number, RIN) was measured by analysis using an Agilent 2100 Bioanalyzer and Agilent RNA 6000 Nano Kit (Agilent Technologies, USA).

Article Title: Tpc1 is an important Zn(II)2Cys6 transcriptional regulator required for polarized growth and virulence in the rice blast fungus
Article Snippet: Paragraph title: RNA isolation and global gene expression profile using microarrays ... RNA quality control was carried out with Agilent RNA 6000 Nano kit (ref. 5067–1504).

Article Title: Anxiety Associated Increased CpG Methylation in the Promoter of Asb1: A Translational Approach Evidenced by Epidemiological and Clinical Studies and a Murine Model
Article Snippet: Paragraph title: Murine Model of Acute Social Defeat Stress and Gene Expression in Mouse Blood ... RNA sample quality was qualitatively analyzed using capillary gel electrophoresis chip (RNA 6000 Nano Kit; Agilent, Boeblingen, Germany).

Modification:

Article Title: Tpc1 is an important Zn(II)2Cys6 transcriptional regulator required for polarized growth and virulence in the rice blast fungus
Article Snippet: RNA isolation and global gene expression profile using microarrays Using a modified protocol of LiCl method[ ], RNA was extracted from 8-day old fungal mycelia grown on cellophane placed on top of CM agar plates ( ). .. RNA quality control was carried out with Agilent RNA 6000 Nano kit (ref. 5067–1504).

Hybridization:

Article Title: Genetic variability in the regulation of gene expression in ten regions of the human brain
Article Snippet: The quality of total RNA was evaluated by the 2100 Bioanalyzer (Agilent) and RNA 6000 Nano Kit (Agilent). .. Processing with the Ambion WT Expression Kit and Affymetrix GeneChip Whole Transcript Sense Target Labeling Assay and hybridization to the Affymetrix Exon 1.0 ST arrays were performed in accordance with the manufacturers’ protocols.

Article Title: Rare coding variants in Phospholipase D3 (PLD3) confer risk for Alzheimer's disease
Article Snippet: .. The quality of total RNA was evaluated by the 2100 Bioanalyzer (Agilent, UK) and RNA 6000 Nano Kit (Agilent, UK) before processing with the Ambion® WT Expression Kit and Affymetrix GeneChip Whole Transcript Sense Target Labeling Assay and hybridization to the Affymetrix Exon 1.0 ST. All arrays were pre-processed using Robust Multi-array Average using Partek Genomics Suite v6.6 (Partek Incorporated, USA). .. The resulting expression data was corrected for individual effects (within which are nested postmortem interval, brain pH, sex, age at death and cause of death) and experimental batch effects (date of hybridization).

Article Title: Microparticle conferred microRNA profiles - implications in the transfer and dominance of cancer traits
Article Snippet: Analysis of RNA integrity and Affymetrix miRNA Arrays Total RNA integrity was analysed using the Agilent RNA 6000 Nano kit (Agilent Technologies Inc., Santa Clara, CA) and the result was analysed by Agilent 2100 Bioanalyzer (Agilent Technologies Inc.) as per the manufacturer’s recommendations; the RNA integrity number (RIN) of 10 represents the highest RNA integrity with minimal degradation and score of 1 is the lowest integrity [ ]. .. RNA labelling, hybridization (Affymetrix™ Fluidics Station 450), scanning (GeneChip® Scanner 3000 7 G) and raw data acquisition of the Affymetrix GeneChip ® miRNA Array (P/N 901326) were performed by Australian Genome Research Facility Ltd, VIC, Australia following a standard procedure from Affymetrix™ (Santa Clara,CA).

RIA Assay:

Article Title: Anxiety Associated Increased CpG Methylation in the Promoter of Asb1: A Translational Approach Evidenced by Epidemiological and Clinical Studies and a Murine Model
Article Snippet: RNA sample quality was qualitatively analyzed using capillary gel electrophoresis chip (RNA 6000 Nano Kit; Agilent, Boeblingen, Germany). .. Plasma was isolated following centrifugation at 6000 g at 4 °C for 15 min. A commercially available radioimmunoassay kit with 125 I-labeled anti-corticosterone antibody was used to determine plasma corticosterone levels (MP Biomedicals, Solon, OH, USA; sensitivity 12.5 ng/ml).

Dissection:

Article Title: Rare coding variants in Phospholipase D3 (PLD3) confer risk for Alzheimer's disease
Article Snippet: All samples originated from individuals with no significant neurological history or neuropathological abnormality and were collected by the MRC Edinburgh Brain Bank ensuring a consistent dissection protocol and sample handling procedure. .. The quality of total RNA was evaluated by the 2100 Bioanalyzer (Agilent, UK) and RNA 6000 Nano Kit (Agilent, UK) before processing with the Ambion® WT Expression Kit and Affymetrix GeneChip Whole Transcript Sense Target Labeling Assay and hybridization to the Affymetrix Exon 1.0 ST. All arrays were pre-processed using Robust Multi-array Average using Partek Genomics Suite v6.6 (Partek Incorporated, USA).

Northern Blot:

Article Title: Blue Light Acts as a Double-Edged Sword in Regulating Sexual Development of Hypocrea jecorina (Trichoderma reesei)
Article Snippet: Paragraph title: Quantification of Gene Expression by Northern Blot and qRT-PCR ... The quality of extracted RNA was further analyzed with the RNA 6000 Nano kit by Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA).

Generated:

Article Title: The SmAP1/2 proteins of the crenarchaeon Sulfolobus solfataricus interact with the exosome and stimulate A-rich tailing of transcripts
Article Snippet: Two microliter of the eluted A-rich RNAs were analyzed with the Agilent 2100 Bioanalyzer (Agilent Technologies) and the RNA 6000 Nano Kit (Agilent Technologies) following the manufacturer's instructions. .. A total of 100 bp single end sequence reads were generated by the next generation sequencing facility at the Vienna Biocenter Core Facilities GmbH (VBCF), member of Vienna Biocenter (VBC), using the Illumina HiSeq 2000 platform.

Sequencing:

Article Title: Cloning and Variation of Ground State Intestinal Stem Cells
Article Snippet: RNA quality (RNA integrity number, RIN) was measured by analysis Agilent 2100 Bioanalyzer and Agilent RNA 6000 Nano Kit (Agilent Technologies, USA). .. Genomic DNA was extracted with DNeasy Blood & Tissue kit (Qiagen, Netherlands) from intestinal and colonic stem cells for CNV analysis and exome capture sequencing.

Article Title: The SmAP1/2 proteins of the crenarchaeon Sulfolobus solfataricus interact with the exosome and stimulate A-rich tailing of transcripts
Article Snippet: Two microliter of the eluted A-rich RNAs were analyzed with the Agilent 2100 Bioanalyzer (Agilent Technologies) and the RNA 6000 Nano Kit (Agilent Technologies) following the manufacturer's instructions. .. A total of 100 bp single end sequence reads were generated by the next generation sequencing facility at the Vienna Biocenter Core Facilities GmbH (VBCF), member of Vienna Biocenter (VBC), using the Illumina HiSeq 2000 platform.

Sonication:

Article Title: Ythdf2-mediated m6A mRNA clearance modulates neural development in mice
Article Snippet: The mRNA quality was checked using a 2100 Bioanalyzer instrument with an Agilent RNA 6000 Nano kit (5067–1511). .. RNA fragmentation (1 μg) was performed by sonication at 10 ng/μl in 100 μl RNase-free water with Bioruptor Pico (Diagenode) with 30 cycles of 30 s on followed by 30 s off; 5% of the fragmented RNA was saved as input. m6 A IP was performed with an EpiMark® N 6 -Methyladenosine Enrichment Kit (NEB, E1610S) following the kit manual adapted for the KingFisher™ Duo Prime Purification System.

DNA Extraction:

Article Title: Cloning and Variation of Ground State Intestinal Stem Cells
Article Snippet: RNA quality (RNA integrity number, RIN) was measured by analysis Agilent 2100 Bioanalyzer and Agilent RNA 6000 Nano Kit (Agilent Technologies, USA). .. For genomic DNA extraction, human intestinal and colonic stem cells were isolated from mouse 3T3 feeder layer using QuadroMACS Starting Kit (Miltenyi Biotec, Germany).

Nucleic Acid Electrophoresis:

Article Title: RNA Catabolites Contribute to the Nitrogen Pool and Support Growth Recovery of Wheat
Article Snippet: .. RNA Quality Assessment RNA quality was assessed by microfluidic gel electrophoresis using an RNA 6000 Nano Kit (Agilent, United States) and an Agilent 2100 Bioanalyzer and performed at the David Gunn Genomics Facility (SAHMRI, Adelaide). ..

Article Title: Anxiety Associated Increased CpG Methylation in the Promoter of Asb1: A Translational Approach Evidenced by Epidemiological and Clinical Studies and a Murine Model
Article Snippet: .. RNA sample quality was qualitatively analyzed using capillary gel electrophoresis chip (RNA 6000 Nano Kit; Agilent, Boeblingen, Germany). .. RNA samples of sufficient quality (RIN⩾7.5) were then processed with the Illumina TotalPrep-96 RNA Amplification Kit (Ambion, Austin, TX, USA) and hybridized onto MouseWG-6 v2 Expression BeadChips (Illumina, San Diego, CA, USA).

Article Title: RNA Catabolites Contribute to the Nitrogen Pool and Support Growth Recovery of Wheat
Article Snippet: .. RNA quality was assessed by microfluidic gel electrophoresis using an RNA 6000 Nano Kit (Agilent, United States) and an Agilent 2100 Bioanalyzer and performed at the David Gunn Genomics Facility (SAHMRI, Adelaide). ..

Magnetic Beads:

Article Title: CAGE profiling of ncRNAs in hepatocellular carcinoma reveals widespread activation of retroviral LTR promoters in virus-induced tumors
Article Snippet: The quality of the RNA samples was assessed using an Agilent RNA 6000 Nano kit (RIN scores provided in Supplemental Table S1). .. Full-length cDNAs were biotinylated and captured by streptavidin-coated magnetic beads.

Isolation:

Article Title: Genetic variability in the regulation of gene expression in ten regions of the human brain
Article Snippet: Paragraph title: RnA isolation and processing brain samples on Affymetrix Human exon 1.0 ST arrays ... The quality of total RNA was evaluated by the 2100 Bioanalyzer (Agilent) and RNA 6000 Nano Kit (Agilent).

Article Title: Rare coding variants in Phospholipase D3 (PLD3) confer risk for Alzheimer's disease
Article Snippet: Total RNA was isolated from human post-mortem brain tissues using the miRNeasy 96 well kit (Qiagen, UK). .. The quality of total RNA was evaluated by the 2100 Bioanalyzer (Agilent, UK) and RNA 6000 Nano Kit (Agilent, UK) before processing with the Ambion® WT Expression Kit and Affymetrix GeneChip Whole Transcript Sense Target Labeling Assay and hybridization to the Affymetrix Exon 1.0 ST. All arrays were pre-processed using Robust Multi-array Average using Partek Genomics Suite v6.6 (Partek Incorporated, USA).

Article Title: Ythdf2-mediated m6A mRNA clearance modulates neural development in mice
Article Snippet: Paragraph title: mRNA isolation and m6 A-RIP ... The mRNA quality was checked using a 2100 Bioanalyzer instrument with an Agilent RNA 6000 Nano kit (5067–1511).

Article Title: Cloning and Variation of Ground State Intestinal Stem Cells
Article Snippet: For ALI-differentiated epithelia, RNA was isolated using Trizol RNA Isolation Kit (Life Technologies, USA). .. RNA quality (RNA integrity number, RIN) was measured by analysis Agilent 2100 Bioanalyzer and Agilent RNA 6000 Nano Kit (Agilent Technologies, USA).

Article Title: Tpc1 is an important Zn(II)2Cys6 transcriptional regulator required for polarized growth and virulence in the rice blast fungus
Article Snippet: Paragraph title: RNA isolation and global gene expression profile using microarrays ... RNA quality control was carried out with Agilent RNA 6000 Nano kit (ref. 5067–1504).

Article Title: Inhibition of stationary phase respiration impairs persister formation in E. coli
Article Snippet: Paragraph title: RNA isolation ... Quality of total RNA was assessed with a bioanalyzer using an RNA 6000 Nano kit (Agilent Technologies, Inc, Santa Clara, CA).

Article Title: Anxiety Associated Increased CpG Methylation in the Promoter of Asb1: A Translational Approach Evidenced by Epidemiological and Clinical Studies and a Murine Model
Article Snippet: RNA sample quality was qualitatively analyzed using capillary gel electrophoresis chip (RNA 6000 Nano Kit; Agilent, Boeblingen, Germany). .. Plasma was isolated following centrifugation at 6000 g at 4 °C for 15 min. A commercially available radioimmunoassay kit with 125 I-labeled anti-corticosterone antibody was used to determine plasma corticosterone levels (MP Biomedicals, Solon, OH, USA; sensitivity 12.5 ng/ml).

Article Title: The SmAP1/2 proteins of the crenarchaeon Sulfolobus solfataricus interact with the exosome and stimulate A-rich tailing of transcripts
Article Snippet: Paragraph title: Isolation of RNA and RNASeq ... Two microliter of the eluted A-rich RNAs were analyzed with the Agilent 2100 Bioanalyzer (Agilent Technologies) and the RNA 6000 Nano Kit (Agilent Technologies) following the manufacturer's instructions.

Labeling:

Article Title: Genetic variability in the regulation of gene expression in ten regions of the human brain
Article Snippet: The quality of total RNA was evaluated by the 2100 Bioanalyzer (Agilent) and RNA 6000 Nano Kit (Agilent). .. Processing with the Ambion WT Expression Kit and Affymetrix GeneChip Whole Transcript Sense Target Labeling Assay and hybridization to the Affymetrix Exon 1.0 ST arrays were performed in accordance with the manufacturers’ protocols.

Article Title: Rare coding variants in Phospholipase D3 (PLD3) confer risk for Alzheimer's disease
Article Snippet: .. The quality of total RNA was evaluated by the 2100 Bioanalyzer (Agilent, UK) and RNA 6000 Nano Kit (Agilent, UK) before processing with the Ambion® WT Expression Kit and Affymetrix GeneChip Whole Transcript Sense Target Labeling Assay and hybridization to the Affymetrix Exon 1.0 ST. All arrays were pre-processed using Robust Multi-array Average using Partek Genomics Suite v6.6 (Partek Incorporated, USA). .. The resulting expression data was corrected for individual effects (within which are nested postmortem interval, brain pH, sex, age at death and cause of death) and experimental batch effects (date of hybridization).

Article Title: Cloning and Variation of Ground State Intestinal Stem Cells
Article Snippet: Expression microarray and bioinformatics Total RNAs obtained from immature colonies and ALI differentiated structure were used for microarray preparation with WT Pico RNA Amplification System V2 for amplification of DNA and Encore Biotin Module for fragmentation and biotin labeling (NuGEN Technologies, USA). .. RNA quality (RNA integrity number, RIN) was measured by analysis using an Agilent 2100 Bioanalyzer and Agilent RNA 6000 Nano Kit (Agilent Technologies, USA).

Purification:

Article Title: Ythdf2-mediated m6A mRNA clearance modulates neural development in mice
Article Snippet: We applied 1 mg total RNA for further mRNA purification with a Dynabeads mRNA DIRECT™ purification kit (Thermo, 61,011) for two rounds. .. The mRNA quality was checked using a 2100 Bioanalyzer instrument with an Agilent RNA 6000 Nano kit (5067–1511).

Article Title: Inhibition of stationary phase respiration impairs persister formation in E. coli
Article Snippet: Total RNA was purified with RNeasy extraction kit using the manufacturer’s protocol (Qiagen). .. Quality of total RNA was assessed with a bioanalyzer using an RNA 6000 Nano kit (Agilent Technologies, Inc, Santa Clara, CA).

Article Title: The SmAP1/2 proteins of the crenarchaeon Sulfolobus solfataricus interact with the exosome and stimulate A-rich tailing of transcripts
Article Snippet: A total of 250 μg of total RNA of each sample (two biological replicates for each condition/strain) was used to isolate adenylated RNAs employing the poly(A) RNA purification kit Oligotex™ (Qiagen) following the manufacturer's instructions. .. Two microliter of the eluted A-rich RNAs were analyzed with the Agilent 2100 Bioanalyzer (Agilent Technologies) and the RNA 6000 Nano Kit (Agilent Technologies) following the manufacturer's instructions.

Construct:

Article Title: The SmAP1/2 proteins of the crenarchaeon Sulfolobus solfataricus interact with the exosome and stimulate A-rich tailing of transcripts
Article Snippet: Two microliter of the eluted A-rich RNAs were analyzed with the Agilent 2100 Bioanalyzer (Agilent Technologies) and the RNA 6000 Nano Kit (Agilent Technologies) following the manufacturer's instructions. .. The cDNAs libraries were constructed using the NEBNext® Ultra™ Directional RNA Library Prep Kit.

Mouse Assay:

Article Title: Anxiety Associated Increased CpG Methylation in the Promoter of Asb1: A Translational Approach Evidenced by Epidemiological and Clinical Studies and a Murine Model
Article Snippet: Exactly 4 h following stress/control exposure, mice were anesthetized with isoflurane and killed. .. RNA sample quality was qualitatively analyzed using capillary gel electrophoresis chip (RNA 6000 Nano Kit; Agilent, Boeblingen, Germany).

Chromatin Immunoprecipitation:

Article Title: Anxiety Associated Increased CpG Methylation in the Promoter of Asb1: A Translational Approach Evidenced by Epidemiological and Clinical Studies and a Murine Model
Article Snippet: .. RNA sample quality was qualitatively analyzed using capillary gel electrophoresis chip (RNA 6000 Nano Kit; Agilent, Boeblingen, Germany). .. RNA samples of sufficient quality (RIN⩾7.5) were then processed with the Illumina TotalPrep-96 RNA Amplification Kit (Ambion, Austin, TX, USA) and hybridized onto MouseWG-6 v2 Expression BeadChips (Illumina, San Diego, CA, USA).

Software:

Article Title: Cloning and Variation of Ground State Intestinal Stem Cells
Article Snippet: RNA quality (RNA integrity number, RIN) was measured by analysis using an Agilent 2100 Bioanalyzer and Agilent RNA 6000 Nano Kit (Agilent Technologies, USA). .. GeneChip operating software was used to process all the Cel files and calculate probe intensity values.

Sample Prep:

Article Title: Cloning and Variation of Ground State Intestinal Stem Cells
Article Snippet: Paragraph title: RNA and genomic DNA sample preparation ... RNA quality (RNA integrity number, RIN) was measured by analysis Agilent 2100 Bioanalyzer and Agilent RNA 6000 Nano Kit (Agilent Technologies, USA).

Next-Generation Sequencing:

Article Title: The SmAP1/2 proteins of the crenarchaeon Sulfolobus solfataricus interact with the exosome and stimulate A-rich tailing of transcripts
Article Snippet: Two microliter of the eluted A-rich RNAs were analyzed with the Agilent 2100 Bioanalyzer (Agilent Technologies) and the RNA 6000 Nano Kit (Agilent Technologies) following the manufacturer's instructions. .. A total of 100 bp single end sequence reads were generated by the next generation sequencing facility at the Vienna Biocenter Core Facilities GmbH (VBCF), member of Vienna Biocenter (VBC), using the Illumina HiSeq 2000 platform.

Spectrophotometry:

Article Title: Microparticle conferred microRNA profiles - implications in the transfer and dominance of cancer traits
Article Snippet: Analysis of RNA integrity and Affymetrix miRNA Arrays Total RNA integrity was analysed using the Agilent RNA 6000 Nano kit (Agilent Technologies Inc., Santa Clara, CA) and the result was analysed by Agilent 2100 Bioanalyzer (Agilent Technologies Inc.) as per the manufacturer’s recommendations; the RNA integrity number (RIN) of 10 represents the highest RNA integrity with minimal degradation and score of 1 is the lowest integrity [ ]. .. Nanodrop-1000 spectrophotometer (Nanodrop technologies, DE, USA) was used for the quantification of RNA and 500 ng of RNA from each sample was used for miRNA microarray analysis.

Staining:

Article Title: Sequence-Specific Cleavage of Small-Subunit (SSU) rRNA with Oligonucleotides and RNase H: a Rapid and Simple Approach to SSU rRNA-Based Quantitative Detection of Microorganisms
Article Snippet: The resultant RNA fragments were electrophoresed by either 1.5% agarose gels (Nusieve 3:1 agarose; BioWhittaker Molecular Applications) or the Agilent 2100 bioanalyzer with the RNA 6000 nano kit (Agilent). .. Nucleic acids in the gels were stained by CYBER GOLD nucleotide-staining dye (Molecular Probes) to make the RNA bands visible.

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    Agilent technologies bioanalyzer rna nano 6000 kit
    <t>Bioanalyzer</t> profiles of the large <t>RNA</t> fractions isolated from donors with the lowest and highest blood RNA level. The large RNA fractions (200 ng) from the low RNA level sample 180 (6.7 μg RNA/ml blood) and the high RNA level sample 162 (22.7 μg RNA/ml blood) were separated using the Bioanalyzer RNA <t>Nano</t> 6000 Kit. Shown are positions of: the (18S) and (28S) ribosomal RNA; the 600 nt globin region (G); and Bioanalyzer marker (M).
    Bioanalyzer Rna Nano 6000 Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bioanalyzer rna nano 6000 kit/product/Agilent technologies
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bioanalyzer rna nano 6000 kit - by Bioz Stars, 2020-01
    85/100 stars
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    99
    Agilent technologies rna 6000 nano kit
    Over-drying time does not affect quantity of small RNA in mirRICH method. (A) 15% PAGE (Polyacrylamide gel electrophoresis) and (B) 15% UREA denaturing gels electrophoresis of the RNA samples are prepared from two different breast cancer cells, MDA-MB-231 and MCF7 cells with either TRIzol with 1hr drying or 24hr drying, mirRICH with 1hr drying or 24hr drying. The 100-bp marker is indicated as ladder. (C) Peak images of experiment samples of MDA-MB-231 and MCF7 were obtained by Aglient <t>RNA</t> 6000 <t>Nano</t> kit respectively.
    Rna 6000 Nano Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 555 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rna 6000 nano kit/product/Agilent technologies
    Average 99 stars, based on 555 article reviews
    Price from $9.99 to $1999.99
    rna 6000 nano kit - by Bioz Stars, 2020-01
    99/100 stars
      Buy from Supplier

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    Bioanalyzer profiles of the large RNA fractions isolated from donors with the lowest and highest blood RNA level. The large RNA fractions (200 ng) from the low RNA level sample 180 (6.7 μg RNA/ml blood) and the high RNA level sample 162 (22.7 μg RNA/ml blood) were separated using the Bioanalyzer RNA Nano 6000 Kit. Shown are positions of: the (18S) and (28S) ribosomal RNA; the 600 nt globin region (G); and Bioanalyzer marker (M).

    Journal: PLoS ONE

    Article Title: Inter-Individual Differences in RNA Levels in Human Peripheral Blood

    doi: 10.1371/journal.pone.0148260

    Figure Lengend Snippet: Bioanalyzer profiles of the large RNA fractions isolated from donors with the lowest and highest blood RNA level. The large RNA fractions (200 ng) from the low RNA level sample 180 (6.7 μg RNA/ml blood) and the high RNA level sample 162 (22.7 μg RNA/ml blood) were separated using the Bioanalyzer RNA Nano 6000 Kit. Shown are positions of: the (18S) and (28S) ribosomal RNA; the 600 nt globin region (G); and Bioanalyzer marker (M).

    Article Snippet: The isolated RNA samples were analyzed with the Agilent Bioanalyzer RNA Nano 6000 Kit (Agilent Technologies, Inc., Santa Clara, CA).

    Techniques: Isolation, Marker

    Characteristics of RNA isolated from healthy donors. a) Proportion of the large and small RNA fractions in relation to increasing quantities of total RNA in human peripheral blood. Samples of RNA from the 35 individuals are sequentially ranked in accord with the increasing amount of total RNA in the sample. For each sample, the amounts of the large RNA fraction (inverted triangle) and small RNA fraction (square) are depicted. b) The large RNA and small RNA fractions are expressed as a percentage of the total RNA in the sample. c) Bioanalyzer RIN values of the large RNA fractions from 35 samples.

    Journal: PLoS ONE

    Article Title: Inter-Individual Differences in RNA Levels in Human Peripheral Blood

    doi: 10.1371/journal.pone.0148260

    Figure Lengend Snippet: Characteristics of RNA isolated from healthy donors. a) Proportion of the large and small RNA fractions in relation to increasing quantities of total RNA in human peripheral blood. Samples of RNA from the 35 individuals are sequentially ranked in accord with the increasing amount of total RNA in the sample. For each sample, the amounts of the large RNA fraction (inverted triangle) and small RNA fraction (square) are depicted. b) The large RNA and small RNA fractions are expressed as a percentage of the total RNA in the sample. c) Bioanalyzer RIN values of the large RNA fractions from 35 samples.

    Article Snippet: The isolated RNA samples were analyzed with the Agilent Bioanalyzer RNA Nano 6000 Kit (Agilent Technologies, Inc., Santa Clara, CA).

    Techniques: Isolation

    Over-drying time does not affect quantity of small RNA in mirRICH method. (A) 15% PAGE (Polyacrylamide gel electrophoresis) and (B) 15% UREA denaturing gels electrophoresis of the RNA samples are prepared from two different breast cancer cells, MDA-MB-231 and MCF7 cells with either TRIzol with 1hr drying or 24hr drying, mirRICH with 1hr drying or 24hr drying. The 100-bp marker is indicated as ladder. (C) Peak images of experiment samples of MDA-MB-231 and MCF7 were obtained by Aglient RNA 6000 Nano kit respectively.

    Journal: RNA Biology

    Article Title: mirRICH, a simple method to enrich the small RNA fraction from over-dried RNA pellets

    doi: 10.1080/15476286.2018.1451723

    Figure Lengend Snippet: Over-drying time does not affect quantity of small RNA in mirRICH method. (A) 15% PAGE (Polyacrylamide gel electrophoresis) and (B) 15% UREA denaturing gels electrophoresis of the RNA samples are prepared from two different breast cancer cells, MDA-MB-231 and MCF7 cells with either TRIzol with 1hr drying or 24hr drying, mirRICH with 1hr drying or 24hr drying. The 100-bp marker is indicated as ladder. (C) Peak images of experiment samples of MDA-MB-231 and MCF7 were obtained by Aglient RNA 6000 Nano kit respectively.

    Article Snippet: To analyze more precisely the size distributions of the small RNA fractions, we used an Agilent 2100 bioanalyzer with the Agilent RNA 6000 Nano kit or small RNA kit according to the manufacturer's protocol.

    Techniques: Polyacrylamide Gel Electrophoresis, Electrophoresis, Multiple Displacement Amplification, Marker

    Comparison of the quantity and the quality of small RNAs isolated by TRIzol, mirRICH and mirVana. Equal number of two different breast cancer cells, MDA-MB-231 and MCF7 cells were prepared and extracted with three different methods: TRIzol, mirRICH, and mirVana. Peak images of small RNA samples were obtained by Aglient RNA 6000 Nano kit (A) and small RNA analysis kit (B) respectively. (C) 15% PAGE (Polyacrylamide gel electrophoresis) and (D) 15% UREA denaturing gels electrophoresis of the RNA samples from two different breast cancer cells, MDA-MB-231 and MCF7 are prepared by three different methods, TRIzol (T), mirRICH (M) and mirVana (K). The 100-bp marker is indicated as ladder.

    Journal: RNA Biology

    Article Title: mirRICH, a simple method to enrich the small RNA fraction from over-dried RNA pellets

    doi: 10.1080/15476286.2018.1451723

    Figure Lengend Snippet: Comparison of the quantity and the quality of small RNAs isolated by TRIzol, mirRICH and mirVana. Equal number of two different breast cancer cells, MDA-MB-231 and MCF7 cells were prepared and extracted with three different methods: TRIzol, mirRICH, and mirVana. Peak images of small RNA samples were obtained by Aglient RNA 6000 Nano kit (A) and small RNA analysis kit (B) respectively. (C) 15% PAGE (Polyacrylamide gel electrophoresis) and (D) 15% UREA denaturing gels electrophoresis of the RNA samples from two different breast cancer cells, MDA-MB-231 and MCF7 are prepared by three different methods, TRIzol (T), mirRICH (M) and mirVana (K). The 100-bp marker is indicated as ladder.

    Article Snippet: To analyze more precisely the size distributions of the small RNA fractions, we used an Agilent 2100 bioanalyzer with the Agilent RNA 6000 Nano kit or small RNA kit according to the manufacturer's protocol.

    Techniques: Isolation, Multiple Displacement Amplification, Polyacrylamide Gel Electrophoresis, Electrophoresis, Marker

    Over-drying time does not affect quantity of small RNA in mirRICH method. (A) 15% PAGE (Polyacrylamide gel electrophoresis) and (B) 15% UREA denaturing gels electrophoresis of the RNA samples are prepared from two different breast cancer cells, MDA-MB-231 and MCF7 cells with either TRIzol with 1hr drying or 24hr drying, mirRICH with 1hr drying or 24hr drying. The 100-bp marker is indicated as ladder. (C) Peak images of experiment samples of MDA-MB-231 and MCF7 were obtained by Aglient RNA 6000 Nano kit respectively.

    Journal: RNA Biology

    Article Title: mirRICH, a simple method to enrich the small RNA fraction from over-dried RNA pellets

    doi: 10.1080/15476286.2018.1451723

    Figure Lengend Snippet: Over-drying time does not affect quantity of small RNA in mirRICH method. (A) 15% PAGE (Polyacrylamide gel electrophoresis) and (B) 15% UREA denaturing gels electrophoresis of the RNA samples are prepared from two different breast cancer cells, MDA-MB-231 and MCF7 cells with either TRIzol with 1hr drying or 24hr drying, mirRICH with 1hr drying or 24hr drying. The 100-bp marker is indicated as ladder. (C) Peak images of experiment samples of MDA-MB-231 and MCF7 were obtained by Aglient RNA 6000 Nano kit respectively.

    Article Snippet: Validation of size and quality of obtained RNA To analyze more precisely the size distributions of the small RNA fractions, we used an Agilent 2100 bioanalyzer with the Agilent RNA 6000 Nano kit or small RNA kit according to the manufacturer's protocol.

    Techniques: Polyacrylamide Gel Electrophoresis, Electrophoresis, Multiple Displacement Amplification, Marker

    Comparison of the quantity and the quality of small RNAs isolated by TRIzol, mirRICH and mirVana. Equal number of two different breast cancer cells, MDA-MB-231 and MCF7 cells were prepared and extracted with three different methods: TRIzol, mirRICH, and mirVana. Peak images of small RNA samples were obtained by Aglient RNA 6000 Nano kit (A) and small RNA analysis kit (B) respectively. (C) 15% PAGE (Polyacrylamide gel electrophoresis) and (D) 15% UREA denaturing gels electrophoresis of the RNA samples from two different breast cancer cells, MDA-MB-231 and MCF7 are prepared by three different methods, TRIzol (T), mirRICH (M) and mirVana (K). The 100-bp marker is indicated as ladder.

    Journal: RNA Biology

    Article Title: mirRICH, a simple method to enrich the small RNA fraction from over-dried RNA pellets

    doi: 10.1080/15476286.2018.1451723

    Figure Lengend Snippet: Comparison of the quantity and the quality of small RNAs isolated by TRIzol, mirRICH and mirVana. Equal number of two different breast cancer cells, MDA-MB-231 and MCF7 cells were prepared and extracted with three different methods: TRIzol, mirRICH, and mirVana. Peak images of small RNA samples were obtained by Aglient RNA 6000 Nano kit (A) and small RNA analysis kit (B) respectively. (C) 15% PAGE (Polyacrylamide gel electrophoresis) and (D) 15% UREA denaturing gels electrophoresis of the RNA samples from two different breast cancer cells, MDA-MB-231 and MCF7 are prepared by three different methods, TRIzol (T), mirRICH (M) and mirVana (K). The 100-bp marker is indicated as ladder.

    Article Snippet: Validation of size and quality of obtained RNA To analyze more precisely the size distributions of the small RNA fractions, we used an Agilent 2100 bioanalyzer with the Agilent RNA 6000 Nano kit or small RNA kit according to the manufacturer's protocol.

    Techniques: Isolation, Multiple Displacement Amplification, Polyacrylamide Gel Electrophoresis, Electrophoresis, Marker