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Agilent technologies rna 6000 labchip kit
Results of RNA 6000 <t>LabChip</t> analysis. RNAs extracted from different fixatives show different RIN and electrophoresis curves.
Rna 6000 Labchip Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Evaluation of two commercial and three home-made fixatives for the substitution of formalin: a formaldehyde-free laboratory is possible"

Article Title: Evaluation of two commercial and three home-made fixatives for the substitution of formalin: a formaldehyde-free laboratory is possible

Journal: Environmental Health

doi: 10.1186/1476-069X-11-59

Results of RNA 6000 LabChip analysis. RNAs extracted from different fixatives show different RIN and electrophoresis curves.
Figure Legend Snippet: Results of RNA 6000 LabChip analysis. RNAs extracted from different fixatives show different RIN and electrophoresis curves.

Techniques Used: Electrophoresis

Related Articles

Centrifugation:

Article Title: IGF1R activation and the in vitro antiproliferative efficacy of IGF1R inhibitor are inversely correlated with IGFBP5 expression in bladder cancer
Article Snippet: RNA, DNA, and proteins were extracted from the surgical samples by cesium chloride density centrifugation. .. The concentration, integrity and purity of each RNA sample were determined with the RNA 6000 LabChip Kit (Agilent Technologies, Massy, France) and an Agilent 2100 bioanalyzer.

Article Title: Microarray analysis of subcutaneous adipose tissue from mature cows with divergent body weight gain after feed restriction and realimentation
Article Snippet: 2.3 Total RNA was extracted from 50 to 100 mg of tissue by homogenization with TriPure reagent (Roche, Indianapolis, IN) following the manufacturer's protocol with the exception of an increased time of centrifugation from 15 to 20 min following the addition of chloroform. .. The quality of total RNA was determined by running samples on a RNA 6000 LabChip kit (Agilent Technologies, Santa Clara, CA) with the Agilent 2100 bioanalyzer.

Amplification:

Article Title: Characterisation of a Novel Fc Conjugate of Macrophage Colony-stimulating Factor
Article Snippet: RNA integrity and quality was assessed using Following the RNA 6000 LabChip Kit (Agilent). .. Microarray was performed by ARK Genomics, The Roslin Institute.

Article Title: Safety of Bifidobacterium animalis subsp. lactis (B. lactis) strain BB-12-supplemented yogurt in healthy adults on antibiotics: a phase I safety study
Article Snippet: DNAse-treated RNA was quantified using the Agilent Bioanalyzer 2100 and RNA 6000 Labchip Kit (Agilent Technologies, Palo Alto, CA). .. The sequence information for human genes assayed can be found in the Porcine Translational Research Database from the Diet, Genomics, and Immunology Laboratory, Beltsville Human Nutrition Research Center, Agricultural Research Service-United States Department of Agriculture ( ).

Article Title: Allele-specific expression in a family quartet with autism reveals mono-to-biallelic switch and novel transcriptional processes of autism susceptibility genes
Article Snippet: The quality of RNA was based on the RNA integrity number (RIN) measured with a Bioanalyzer 2100 (Agilent Technology) and an RNA 6000 LabChip Kit (Agilent Technology). .. Ribosomal RNA was removed from the purified RNA with the Ribo-Zero Magnetic Gold Kit (Epicentre, MRZG126).

Article Title: Analysis of the Serotonergic System in a Mouse Model of Rett Syndrome Reveals Unusual Upregulation of Serotonin Receptor 5b
Article Snippet: The total ribonucleic acid (RNA) of homogenized brain tissue (hippocampus and brainstem) was isolated using the Trizol® method according to manufacturer’s instructions (GibcoBRL) and its concentration was determined using the nanodrop ND-1000 spectrophotometer followed by its quality and integrity measurement by electrophoresis on RNA 6000 LabChip® kit (Agilent 2100 Bioanalyzer). .. The total ribonucleic acid (RNA) of homogenized brain tissue (hippocampus and brainstem) was isolated using the Trizol® method according to manufacturer’s instructions (GibcoBRL) and its concentration was determined using the nanodrop ND-1000 spectrophotometer followed by its quality and integrity measurement by electrophoresis on RNA 6000 LabChip® kit (Agilent 2100 Bioanalyzer).

Article Title: Srf destabilizes cellular identity by suppressing cell-type-specific gene expression programs
Article Snippet: Total RNA was extracted using RNeasy Mini Kit and assessed using RNA 6000 LabChip kit and Agilent 2100 Bioanalyzer (Agilent Technologies). .. Total RNA was extracted using RNeasy Mini Kit and assessed using RNA 6000 LabChip kit and Agilent 2100 Bioanalyzer (Agilent Technologies).

Article Title: The first whole genome and transcriptome of the cinereous vulture reveals adaptation in the gastric and immune defense systems and possible convergent evolution between the Old and New World vultures
Article Snippet: RNA quality was assessed by analysis of rRNA band integrity on an Agilent RNA 6000 LabChip kit (Agilent Technologies). .. The cDNAs were subjected to end-repair and poly (A) addition, and then connected with sequencing adapters using the TruSeq RNA Sample Prep Kit (Illumina).

Synthesized:

Article Title: Safety of Bifidobacterium animalis subsp. lactis (B. lactis) strain BB-12-supplemented yogurt in healthy adults on antibiotics: a phase I safety study
Article Snippet: The mRNA expression level of selected genes associated with transcription factors: T-box transcription factor ( TBX21 ), GATA binding protein 3 ( GATA 3 ), Forkhead box P3 ( FOXP3 ), Interferon regulatory factor ( IRF8 ); Toll like receptors TLR2 , TLR4 and TLR9 ; pro-inflammatory and regulatory cytokines: Tumor necrosis factor α ( TNFA ), Interleukin 6 ( IL6 ), Interleukin 1b ( IL1B ), Tumor necrosis factor ligand superfamily member 14 ( TNFSF14 ), interferon gamma ( IFNg ), Tumor growth factor β-2 ( TGFB2 ), Interleukin 10 ( IL10 ); cell activation markers: CD40, HLA-DRA, CD80, CD86, CD274 ; and chemokines CXCL10, CCL3, CCL4 was measured on cDNA synthesized from each sample using 1 μg of total RNA. .. DNAse-treated RNA was quantified using the Agilent Bioanalyzer 2100 and RNA 6000 Labchip Kit (Agilent Technologies, Palo Alto, CA).

Article Title: The first whole genome and transcriptome of the cinereous vulture reveals adaptation in the gastric and immune defense systems and possible convergent evolution between the Old and New World vultures
Article Snippet: RNA quality was assessed by analysis of rRNA band integrity on an Agilent RNA 6000 LabChip kit (Agilent Technologies). .. Prior to cDNA library construction, 2 μg of total RNA was treated with DNase I, and magnetic beads with Oligo (dT) were used to enrich poly (A) mRNA.

Construct:

Article Title: A 40 kDa protein of the inner membrane is the mitochondrial calcium uniporter
Article Snippet: The RNA was quantified and controlled for its quality using the RNA 6000 LabChip kit (Agilent Technologies) in conjunction with an Agilent Bioanalyzer 2001. .. The RNA was quantified and controlled for its quality using the RNA 6000 LabChip kit (Agilent Technologies) in conjunction with an Agilent Bioanalyzer 2001.

Real-time Polymerase Chain Reaction:

Article Title: A 40 kDa protein of the inner membrane is the mitochondrial calcium uniporter
Article Snippet: The RNA was quantified and controlled for its quality using the RNA 6000 LabChip kit (Agilent Technologies) in conjunction with an Agilent Bioanalyzer 2001. .. The RNA was quantified and controlled for its quality using the RNA 6000 LabChip kit (Agilent Technologies) in conjunction with an Agilent Bioanalyzer 2001.

Article Title: Development and Characterization of an Effective Food Allergy Model in Brown Norway Rats
Article Snippet: The quality of the RNA was assessed by the Agilent 2100 Bioanalyzer with the RNA 6000 LabChip kit (Agilent Technologies, Madrid, Spain). .. The quality of the RNA was assessed by the Agilent 2100 Bioanalyzer with the RNA 6000 LabChip kit (Agilent Technologies, Madrid, Spain).

Article Title: Gene Network Analysis of Glucose Linked Signaling Pathways and Their Role in Human Hepatocellular Carcinoma Cell Growth and Survival in HuH7 and HepG2 Cell Lines
Article Snippet: Paragraph title: 2.4. Messenger RNA Quantification by Real-Time Quantitative PCR (RT-qPCR) ... RNA integrity was assessed with the Agilent 2100 Bioanalyzer and RNA 6000 LabChip Kit (Agilent Technologies, Massy, France).

Article Title: Safety of Bifidobacterium animalis subsp. lactis (B. lactis) strain BB-12-supplemented yogurt in healthy adults on antibiotics: a phase I safety study
Article Snippet: DNAse-treated RNA was quantified using the Agilent Bioanalyzer 2100 and RNA 6000 Labchip Kit (Agilent Technologies, Palo Alto, CA). .. Briefly, cDNA was synthesized with Superscript RT (Invitrogen) and oligo (dT), and 50 ng of this cDNA was used for real-time PCR amplifications using a Thermo-Start DNA Polymerase master mix (Abgene, Rochester, NY) and the ABI PRISM 7700 Sequence detector system (Applied Biosystems, Foster City, CA).

Article Title: White Adipose Tissue Resilience to Insulin Deprivation and Replacement
Article Snippet: RNA integrity was assessed with the Agilent 2100 Bioanalyzer and RNA 6000 LabChip Kit (Agilent Technologies, Massy, France). .. RNA integrity was assessed with the Agilent 2100 Bioanalyzer and RNA 6000 LabChip Kit (Agilent Technologies, Massy, France).

Microarray:

Article Title: Characterisation of a Novel Fc Conjugate of Macrophage Colony-stimulating Factor
Article Snippet: RNA integrity and quality was assessed using Following the RNA 6000 LabChip Kit (Agilent). .. RNA integrity and quality was assessed using Following the RNA 6000 LabChip Kit (Agilent).

Article Title: Transcriptome Analysis of PPAR? Target Genes Reveals the Involvement of Lysyl Oxidase in Human Placental Cytotrophoblast Invasion
Article Snippet: Paragraph title: Microarray analysis ... RNA integrity and purity were checked using a 2100 Bioanalyzer with the RNA 6000 LabChip kit (Agilent Technologies, Massy, France).

Article Title: Srf destabilizes cellular identity by suppressing cell-type-specific gene expression programs
Article Snippet: Paragraph title: Microarray ... Total RNA was extracted using RNeasy Mini Kit and assessed using RNA 6000 LabChip kit and Agilent 2100 Bioanalyzer (Agilent Technologies).

Incubation:

Article Title: Evaluation of two commercial and three home-made fixatives for the substitution of formalin: a formaldehyde-free laboratory is possible
Article Snippet: Samples were subsequently transferred to new tubes containing 5 μl of glycogen (1 mg/ml), precipitated with 3 volumes of ethanol-LiCl, and incubated overnight at – 20°C. .. RNA samples were also tested for their quality using the RNA 6000 LabChip kit (Agilent Technologies) following the manufacturer’s instructions, and RNA analysis was evaluated with the 2100 Bioanalyzer (Agilent Technologies).

Expressing:

Article Title: A 40 kDa protein of the inner membrane is the mitochondrial calcium uniporter
Article Snippet: Paragraph title: RNA extraction and Gene Expression Analyses ... The RNA was quantified and controlled for its quality using the RNA 6000 LabChip kit (Agilent Technologies) in conjunction with an Agilent Bioanalyzer 2001.

Article Title: Development and Characterization of an Effective Food Allergy Model in Brown Norway Rats
Article Snippet: Paragraph title: Quantification of gene expression in small intestine ... The quality of the RNA was assessed by the Agilent 2100 Bioanalyzer with the RNA 6000 LabChip kit (Agilent Technologies, Madrid, Spain).

Article Title: Gene Network Analysis of Glucose Linked Signaling Pathways and Their Role in Human Hepatocellular Carcinoma Cell Growth and Survival in HuH7 and HepG2 Cell Lines
Article Snippet: RNA integrity was assessed with the Agilent 2100 Bioanalyzer and RNA 6000 LabChip Kit (Agilent Technologies, Massy, France). .. Real-time quantitative PCR (RT-qPCR) was performed using ABsolute QPCR SYBR Green ROX Mix (Abgene, Courtaboeuf, France) with a Rotor-GeneTM 6000 system (Corbett Life Science, Cambridgeshire, UK).

Article Title: Transcriptome Analysis of PPAR? Target Genes Reveals the Involvement of Lysyl Oxidase in Human Placental Cytotrophoblast Invasion
Article Snippet: RNA integrity and purity were checked using a 2100 Bioanalyzer with the RNA 6000 LabChip kit (Agilent Technologies, Massy, France). .. RNA integrity and purity were checked using a 2100 Bioanalyzer with the RNA 6000 LabChip kit (Agilent Technologies, Massy, France).

Article Title: Safety of Bifidobacterium animalis subsp. lactis (B. lactis) strain BB-12-supplemented yogurt in healthy adults on antibiotics: a phase I safety study
Article Snippet: The mRNA expression level of selected genes associated with transcription factors: T-box transcription factor ( TBX21 ), GATA binding protein 3 ( GATA 3 ), Forkhead box P3 ( FOXP3 ), Interferon regulatory factor ( IRF8 ); Toll like receptors TLR2 , TLR4 and TLR9 ; pro-inflammatory and regulatory cytokines: Tumor necrosis factor α ( TNFA ), Interleukin 6 ( IL6 ), Interleukin 1b ( IL1B ), Tumor necrosis factor ligand superfamily member 14 ( TNFSF14 ), interferon gamma ( IFNg ), Tumor growth factor β-2 ( TGFB2 ), Interleukin 10 ( IL10 ); cell activation markers: CD40, HLA-DRA, CD80, CD86, CD274 ; and chemokines CXCL10, CCL3, CCL4 was measured on cDNA synthesized from each sample using 1 μg of total RNA. .. DNAse-treated RNA was quantified using the Agilent Bioanalyzer 2100 and RNA 6000 Labchip Kit (Agilent Technologies, Palo Alto, CA).

Article Title: Analysis of the Serotonergic System in a Mouse Model of Rett Syndrome Reveals Unusual Upregulation of Serotonin Receptor 5b
Article Snippet: Gene expression was analyzed by quantitative RT-PCR analysis. .. The total ribonucleic acid (RNA) of homogenized brain tissue (hippocampus and brainstem) was isolated using the Trizol® method according to manufacturer’s instructions (GibcoBRL) and its concentration was determined using the nanodrop ND-1000 spectrophotometer followed by its quality and integrity measurement by electrophoresis on RNA 6000 LabChip® kit (Agilent 2100 Bioanalyzer).

Hybridization:

Article Title: Characterisation of a Novel Fc Conjugate of Macrophage Colony-stimulating Factor
Article Snippet: RNA integrity and quality was assessed using Following the RNA 6000 LabChip Kit (Agilent). .. Total RNA of 50 ng was amplified using the Nugen Pico SL kit, 2.5 ug of the resulting cDNA was biotin labeled using the Nugen Encore labeling kit using the half volume protocol.

Article Title: Systems analysis identifies melanoma-enriched pro-oncogenic networks controlled by the RNA binding protein CELF1
Article Snippet: Quality assessment of Total RNA samples were analyzed using the Agilent Bioanalyzer 2100 and the RNA 6000 LabChip Kit (Agilent) with the Eukaryote Total RNA Nano Assay (Agilent). .. Quality assessment of Total RNA samples were analyzed using the Agilent Bioanalyzer 2100 and the RNA 6000 LabChip Kit (Agilent) with the Eukaryote Total RNA Nano Assay (Agilent).

Flow Cytometry:

Article Title: Allele-specific expression in a family quartet with autism reveals mono-to-biallelic switch and novel transcriptional processes of autism susceptibility genes
Article Snippet: The quality of RNA was based on the RNA integrity number (RIN) measured with a Bioanalyzer 2100 (Agilent Technology) and an RNA 6000 LabChip Kit (Agilent Technology). .. Purified RNA was then amplified and prepared for sequencing with a SureSelect Strand-Specific RNA Library Prep Kit (Agilent Technologies, G9691A).

Sequencing:

Article Title: Development and Characterization of an Effective Food Allergy Model in Brown Norway Rats
Article Snippet: The quality of the RNA was assessed by the Agilent 2100 Bioanalyzer with the RNA 6000 LabChip kit (Agilent Technologies, Madrid, Spain). .. The quality of the RNA was assessed by the Agilent 2100 Bioanalyzer with the RNA 6000 LabChip kit (Agilent Technologies, Madrid, Spain).

Article Title: Safety of Bifidobacterium animalis subsp. lactis (B. lactis) strain BB-12-supplemented yogurt in healthy adults on antibiotics: a phase I safety study
Article Snippet: DNAse-treated RNA was quantified using the Agilent Bioanalyzer 2100 and RNA 6000 Labchip Kit (Agilent Technologies, Palo Alto, CA). .. DNAse-treated RNA was quantified using the Agilent Bioanalyzer 2100 and RNA 6000 Labchip Kit (Agilent Technologies, Palo Alto, CA).

Article Title: Allele-specific expression in a family quartet with autism reveals mono-to-biallelic switch and novel transcriptional processes of autism susceptibility genes
Article Snippet: The quality of RNA was based on the RNA integrity number (RIN) measured with a Bioanalyzer 2100 (Agilent Technology) and an RNA 6000 LabChip Kit (Agilent Technology). .. Ribosomal RNA was removed from the purified RNA with the Ribo-Zero Magnetic Gold Kit (Epicentre, MRZG126).

Article Title: The first whole genome and transcriptome of the cinereous vulture reveals adaptation in the gastric and immune defense systems and possible convergent evolution between the Old and New World vultures
Article Snippet: Paragraph title: Sample preparation and sequencing ... RNA quality was assessed by analysis of rRNA band integrity on an Agilent RNA 6000 LabChip kit (Agilent Technologies).

Concentration Assay:

Article Title: Evaluation of two commercial and three home-made fixatives for the substitution of formalin: a formaldehyde-free laboratory is possible
Article Snippet: RNA concentration was determined at 260 nm and 280 nm with a Thermo Scientific Nanodrop 2000 spectrophotometer (EuroClone, Italy). .. RNA samples were also tested for their quality using the RNA 6000 LabChip kit (Agilent Technologies) following the manufacturer’s instructions, and RNA analysis was evaluated with the 2100 Bioanalyzer (Agilent Technologies).

Article Title: IGF1R activation and the in vitro antiproliferative efficacy of IGF1R inhibitor are inversely correlated with IGFBP5 expression in bladder cancer
Article Snippet: The RNA and DNA were further purified by phenol–chloroform extraction and ethanol precipitation, and the proteins were dialyzed and lyophilized. .. The concentration, integrity and purity of each RNA sample were determined with the RNA 6000 LabChip Kit (Agilent Technologies, Massy, France) and an Agilent 2100 bioanalyzer. .. DNA purity was also assessed by determining the ratio of absorbances at 260 and 280 nm.

Article Title: Inhalation Anesthesia-Induced Neuronal Damage and Gene Expression Changes in Developing Rat Brain
Article Snippet: The yielded RNA concentration was measured spectrophotometrically (NanoDrop® ND-1000; NanoDrop Technologies, Wilmington, DE, USA). .. The integrity of total RNA was evaluated using the RNA 6000 LabChip kit and Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA, USA).

Article Title: The Serotonin Receptor Subtype 5b Specifically Interacts with Serotonin Receptor Subtype 1A
Article Snippet: The primers used for generation of different variants of Htr1a -Htr5b hybrid receptor variants are given in Table . .. The total RNA of homogenized tissue from specific brain regions was isolated using the Trizol® method according to manufacturer’s instructions (GibcoBRL) and its concentration was determined using the nanodrop ND-1000 spectrophotometer followed by its quality and integrity measurement by electrophoresis on RNA 6000 LabChip® kit (Agilent 2100 Bioanalyzer). .. The RNA was transcribed into the cDNA using the iScript cDNA Synthesis Kit (BioRad).

Article Title: Analysis of the Serotonergic System in a Mouse Model of Rett Syndrome Reveals Unusual Upregulation of Serotonin Receptor 5b
Article Snippet: Gene expression was analyzed by quantitative RT-PCR analysis. .. The total ribonucleic acid (RNA) of homogenized brain tissue (hippocampus and brainstem) was isolated using the Trizol® method according to manufacturer’s instructions (GibcoBRL) and its concentration was determined using the nanodrop ND-1000 spectrophotometer followed by its quality and integrity measurement by electrophoresis on RNA 6000 LabChip® kit (Agilent 2100 Bioanalyzer). .. The RNA was transcribed into the corresponding deoxyribonucleic acid (cDNA) using the iScript cDNA Synthesis Kit (BioRad).

Protease Inhibitor:

Article Title: IGF1R activation and the in vitro antiproliferative efficacy of IGF1R inhibitor are inversely correlated with IGFBP5 expression in bladder cancer
Article Snippet: The concentration, integrity and purity of each RNA sample were determined with the RNA 6000 LabChip Kit (Agilent Technologies, Massy, France) and an Agilent 2100 bioanalyzer. .. The concentration, integrity and purity of each RNA sample were determined with the RNA 6000 LabChip Kit (Agilent Technologies, Massy, France) and an Agilent 2100 bioanalyzer.

Generated:

Article Title: Allele-specific expression in a family quartet with autism reveals mono-to-biallelic switch and novel transcriptional processes of autism susceptibility genes
Article Snippet: The quality of RNA was based on the RNA integrity number (RIN) measured with a Bioanalyzer 2100 (Agilent Technology) and an RNA 6000 LabChip Kit (Agilent Technology). .. Libraries were sequenced using sequencing-by-synthesis technology (TruSeq SBS Kit v3-HS, Illumina, FC-401-3001) on an Illumina HiSeq.

Polymerase Chain Reaction:

Article Title: Development and Characterization of an Effective Food Allergy Model in Brown Norway Rats
Article Snippet: The quality of the RNA was assessed by the Agilent 2100 Bioanalyzer with the RNA 6000 LabChip kit (Agilent Technologies, Madrid, Spain). .. The quality of the RNA was assessed by the Agilent 2100 Bioanalyzer with the RNA 6000 LabChip kit (Agilent Technologies, Madrid, Spain).

Article Title: Analysis of the Serotonergic System in a Mouse Model of Rett Syndrome Reveals Unusual Upregulation of Serotonin Receptor 5b
Article Snippet: The total ribonucleic acid (RNA) of homogenized brain tissue (hippocampus and brainstem) was isolated using the Trizol® method according to manufacturer’s instructions (GibcoBRL) and its concentration was determined using the nanodrop ND-1000 spectrophotometer followed by its quality and integrity measurement by electrophoresis on RNA 6000 LabChip® kit (Agilent 2100 Bioanalyzer). .. The total ribonucleic acid (RNA) of homogenized brain tissue (hippocampus and brainstem) was isolated using the Trizol® method according to manufacturer’s instructions (GibcoBRL) and its concentration was determined using the nanodrop ND-1000 spectrophotometer followed by its quality and integrity measurement by electrophoresis on RNA 6000 LabChip® kit (Agilent 2100 Bioanalyzer).

Article Title: The first whole genome and transcriptome of the cinereous vulture reveals adaptation in the gastric and immune defense systems and possible convergent evolution between the Old and New World vultures
Article Snippet: RNA quality was assessed by analysis of rRNA band integrity on an Agilent RNA 6000 LabChip kit (Agilent Technologies). .. The cDNAs were subjected to end-repair and poly (A) addition, and then connected with sequencing adapters using the TruSeq RNA Sample Prep Kit (Illumina).

Binding Assay:

Article Title: Safety of Bifidobacterium animalis subsp. lactis (B. lactis) strain BB-12-supplemented yogurt in healthy adults on antibiotics: a phase I safety study
Article Snippet: The mRNA expression level of selected genes associated with transcription factors: T-box transcription factor ( TBX21 ), GATA binding protein 3 ( GATA 3 ), Forkhead box P3 ( FOXP3 ), Interferon regulatory factor ( IRF8 ); Toll like receptors TLR2 , TLR4 and TLR9 ; pro-inflammatory and regulatory cytokines: Tumor necrosis factor α ( TNFA ), Interleukin 6 ( IL6 ), Interleukin 1b ( IL1B ), Tumor necrosis factor ligand superfamily member 14 ( TNFSF14 ), interferon gamma ( IFNg ), Tumor growth factor β-2 ( TGFB2 ), Interleukin 10 ( IL10 ); cell activation markers: CD40, HLA-DRA, CD80, CD86, CD274 ; and chemokines CXCL10, CCL3, CCL4 was measured on cDNA synthesized from each sample using 1 μg of total RNA. .. DNAse-treated RNA was quantified using the Agilent Bioanalyzer 2100 and RNA 6000 Labchip Kit (Agilent Technologies, Palo Alto, CA).

DNA Extraction:

Article Title: An integrative genomic analysis revealed the relevance of microRNA and gene expression for drug-resistance in human breast cancer cells
Article Snippet: Paragraph title: RNA and genomic DNA extraction ... The quality of the total RNA was verified to have an RNA Integrity Number using a Bioanalyzer and RNA 6000 LabChip Kit (Agilent Technologies).

Nucleic Acid Electrophoresis:

Article Title: Analysis of the Serotonergic System in a Mouse Model of Rett Syndrome Reveals Unusual Upregulation of Serotonin Receptor 5b
Article Snippet: The total ribonucleic acid (RNA) of homogenized brain tissue (hippocampus and brainstem) was isolated using the Trizol® method according to manufacturer’s instructions (GibcoBRL) and its concentration was determined using the nanodrop ND-1000 spectrophotometer followed by its quality and integrity measurement by electrophoresis on RNA 6000 LabChip® kit (Agilent 2100 Bioanalyzer). .. The total ribonucleic acid (RNA) of homogenized brain tissue (hippocampus and brainstem) was isolated using the Trizol® method according to manufacturer’s instructions (GibcoBRL) and its concentration was determined using the nanodrop ND-1000 spectrophotometer followed by its quality and integrity measurement by electrophoresis on RNA 6000 LabChip® kit (Agilent 2100 Bioanalyzer).

RNA Sequencing Assay:

Article Title: Allele-specific expression in a family quartet with autism reveals mono-to-biallelic switch and novel transcriptional processes of autism susceptibility genes
Article Snippet: Paragraph title: RNA extraction and RNA sequencing (RNA-Seq) ... The quality of RNA was based on the RNA integrity number (RIN) measured with a Bioanalyzer 2100 (Agilent Technology) and an RNA 6000 LabChip Kit (Agilent Technology).

Article Title: Deduction of novel genes potentially involved in hypoxic AC16 human cardiomyocytes using next-generation sequencing and bioinformatics approaches
Article Snippet: Paragraph title: RNA sequencing ... The purified RNA was measured at OD260nm with an ND-1000 spectrophotometer (Nanodrop Technologies; Thermo Fisher Scientific, Inc., Wilmington, DE, USA) and qualitatively analyzed with an RNA 6000 LabChip kit (Agilent Technologies, Inc., Santa Clara, CA, USA) and the Bioanalyzer 2100 (Agilent Technologies, Inc.).

Fluorescence:

Article Title: IGF1R activation and the in vitro antiproliferative efficacy of IGF1R inhibitor are inversely correlated with IGFBP5 expression in bladder cancer
Article Snippet: The concentration, integrity and purity of each RNA sample were determined with the RNA 6000 LabChip Kit (Agilent Technologies, Massy, France) and an Agilent 2100 bioanalyzer. .. The concentration, integrity and purity of each RNA sample were determined with the RNA 6000 LabChip Kit (Agilent Technologies, Massy, France) and an Agilent 2100 bioanalyzer.

Article Title: Safety of Bifidobacterium animalis subsp. lactis (B. lactis) strain BB-12-supplemented yogurt in healthy adults on antibiotics: a phase I safety study
Article Snippet: DNAse-treated RNA was quantified using the Agilent Bioanalyzer 2100 and RNA 6000 Labchip Kit (Agilent Technologies, Palo Alto, CA). .. The sequence information for human genes assayed can be found in the Porcine Translational Research Database from the Diet, Genomics, and Immunology Laboratory, Beltsville Human Nutrition Research Center, Agricultural Research Service-United States Department of Agriculture ( ).

Isolation:

Article Title: Population and individual-specific regulatory variation in Sardinia
Article Snippet: For each participant, leukocytes were isolated from whole blood using the LeukoLOCK™ fractionation kit and RNA was extracted using TRI Reagent® (Ambion #AM9738) and isolated using the PureLink® RNA Mini Kit (Ambion #12183018A). .. The quantity and the integrity of isolated RNA samples was evaluated using the Agilent Technologies 2100 Bioanalyzer platform with the RNA 6000 LabChip® kit (Agilent #5067-1511) - samples with an RNA integrity number (RIN) less than 7.5 were discarded. .. Poly-A+ RNA was isolated from 4μg of high-quality total RNA samples through two rounds of positive selection and purification using magnetic beads following the TruSeq RNA Sample Preparation manual (Illumina #15015050).

Article Title: A 40 kDa protein of the inner membrane is the mitochondrial calcium uniporter
Article Snippet: Total RNA was purified using SV Total RNA Isolation kit (Promega) following manufacturer instructions. .. The RNA was quantified and controlled for its quality using the RNA 6000 LabChip kit (Agilent Technologies) in conjunction with an Agilent Bioanalyzer 2001.

Article Title: Development and Characterization of an Effective Food Allergy Model in Brown Norway Rats
Article Snippet: Tissue samples were homogenized in a FastPrep (MP Biomedicals, Illkirch, France) for 30 s. Total RNA was isolated with the RNeasy Mini Kit (Qiagen, Madrid, Spain) following the manufacturer’s recommendations. .. The quality of the RNA was assessed by the Agilent 2100 Bioanalyzer with the RNA 6000 LabChip kit (Agilent Technologies, Madrid, Spain).

Article Title: Safety of Bifidobacterium animalis subsp. lactis (B. lactis) strain BB-12-supplemented yogurt in healthy adults on antibiotics: a phase I safety study
Article Snippet: Whole blood collected at baseline, day 7 and day 14 in PAXgene Blood RNA tubes (BD, New Jersey, USA) was processed for isolation of RNA with on-column DNase digestion following the manufacturer's manual. .. DNAse-treated RNA was quantified using the Agilent Bioanalyzer 2100 and RNA 6000 Labchip Kit (Agilent Technologies, Palo Alto, CA).

Article Title: White Adipose Tissue Resilience to Insulin Deprivation and Replacement
Article Snippet: Total RNA purifications were performed on either 5.105 isolated adipocytes or 100 mg of frozen tissue according to standard protocol (Lipid Quick prep mRNA, Qiagen, Courtaboeuf, France) including a DNase treatment. .. RNA integrity was assessed with the Agilent 2100 Bioanalyzer and RNA 6000 LabChip Kit (Agilent Technologies, Massy, France).

Article Title: Inhalation Anesthesia-Induced Neuronal Damage and Gene Expression Changes in Developing Rat Brain
Article Snippet: Paragraph title: 2.3 Brain tissue collection and RNA isolation ... The integrity of total RNA was evaluated using the RNA 6000 LabChip kit and Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA, USA).

Article Title: The Serotonin Receptor Subtype 5b Specifically Interacts with Serotonin Receptor Subtype 1A
Article Snippet: The primers used for generation of different variants of Htr1a -Htr5b hybrid receptor variants are given in Table . .. The total RNA of homogenized tissue from specific brain regions was isolated using the Trizol® method according to manufacturer’s instructions (GibcoBRL) and its concentration was determined using the nanodrop ND-1000 spectrophotometer followed by its quality and integrity measurement by electrophoresis on RNA 6000 LabChip® kit (Agilent 2100 Bioanalyzer). .. The RNA was transcribed into the cDNA using the iScript cDNA Synthesis Kit (BioRad).

Article Title: Analysis of the Serotonergic System in a Mouse Model of Rett Syndrome Reveals Unusual Upregulation of Serotonin Receptor 5b
Article Snippet: Gene expression was analyzed by quantitative RT-PCR analysis. .. The total ribonucleic acid (RNA) of homogenized brain tissue (hippocampus and brainstem) was isolated using the Trizol® method according to manufacturer’s instructions (GibcoBRL) and its concentration was determined using the nanodrop ND-1000 spectrophotometer followed by its quality and integrity measurement by electrophoresis on RNA 6000 LabChip® kit (Agilent 2100 Bioanalyzer). .. The RNA was transcribed into the corresponding deoxyribonucleic acid (cDNA) using the iScript cDNA Synthesis Kit (BioRad).

Article Title: Microarray analysis of subcutaneous adipose tissue from mature cows with divergent body weight gain after feed restriction and realimentation
Article Snippet: Paragraph title: RNA isolation and quality assessment ... The quality of total RNA was determined by running samples on a RNA 6000 LabChip kit (Agilent Technologies, Santa Clara, CA) with the Agilent 2100 bioanalyzer.

Labeling:

Article Title: Characterisation of a Novel Fc Conjugate of Macrophage Colony-stimulating Factor
Article Snippet: RNA integrity and quality was assessed using Following the RNA 6000 LabChip Kit (Agilent). .. Microarray was performed by ARK Genomics, The Roslin Institute.

Article Title: Srf destabilizes cellular identity by suppressing cell-type-specific gene expression programs
Article Snippet: Total RNA was extracted using RNeasy Mini Kit and assessed using RNA 6000 LabChip kit and Agilent 2100 Bioanalyzer (Agilent Technologies). .. For Agilent Technologies products, 200 ng of total RNA was reverse-transcribed and amplified using Low RNA Input Fluorescent Linear Amplification Kit.

Purification:

Article Title: A 40 kDa protein of the inner membrane is the mitochondrial calcium uniporter
Article Snippet: Total RNA was purified using SV Total RNA Isolation kit (Promega) following manufacturer instructions. .. The RNA was quantified and controlled for its quality using the RNA 6000 LabChip kit (Agilent Technologies) in conjunction with an Agilent Bioanalyzer 2001.

Article Title: IGF1R activation and the in vitro antiproliferative efficacy of IGF1R inhibitor are inversely correlated with IGFBP5 expression in bladder cancer
Article Snippet: The RNA and DNA were further purified by phenol–chloroform extraction and ethanol precipitation, and the proteins were dialyzed and lyophilized. .. The concentration, integrity and purity of each RNA sample were determined with the RNA 6000 LabChip Kit (Agilent Technologies, Massy, France) and an Agilent 2100 bioanalyzer.

Article Title: Systems analysis identifies melanoma-enriched pro-oncogenic networks controlled by the RNA binding protein CELF1
Article Snippet: Total RNA was purified using RNeasy Mini kit (Qiagen) and digested with DNase (Qiagen). .. Quality assessment of Total RNA samples were analyzed using the Agilent Bioanalyzer 2100 and the RNA 6000 LabChip Kit (Agilent) with the Eukaryote Total RNA Nano Assay (Agilent).

Article Title: Transcriptome Analysis of PPAR? Target Genes Reveals the Involvement of Lysyl Oxidase in Human Placental Cytotrophoblast Invasion
Article Snippet: RNA was extracted using the TRIzol® reagent (Invitrogen) as described for RT-PCR, and then purified using the RNeasy® Mini kit (Quiagen S.A., Courtabœuf, France). .. RNA integrity and purity were checked using a 2100 Bioanalyzer with the RNA 6000 LabChip kit (Agilent Technologies, Massy, France).

Article Title: Allele-specific expression in a family quartet with autism reveals mono-to-biallelic switch and novel transcriptional processes of autism susceptibility genes
Article Snippet: The quality of RNA was based on the RNA integrity number (RIN) measured with a Bioanalyzer 2100 (Agilent Technology) and an RNA 6000 LabChip Kit (Agilent Technology). .. Ribosomal RNA was removed from the purified RNA with the Ribo-Zero Magnetic Gold Kit (Epicentre, MRZG126).

Article Title: Microarray analysis of subcutaneous adipose tissue from mature cows with divergent body weight gain after feed restriction and realimentation
Article Snippet: Purified RNA was quantified using a NanoDrop 8000 spectrophotometer (Thermo Scientific, Wilmington, DE). .. The quality of total RNA was determined by running samples on a RNA 6000 LabChip kit (Agilent Technologies, Santa Clara, CA) with the Agilent 2100 bioanalyzer.

Article Title: Deduction of novel genes potentially involved in hypoxic AC16 human cardiomyocytes using next-generation sequencing and bioinformatics approaches
Article Snippet: Total RNAs from the normoxic and hypoxic AC16 CMs were extracted with TRIzol reagent (Invitrogen, Thermo Fisher Scientific, Inc.) following the manufacturer's standard protocol. .. The purified RNA was measured at OD260nm with an ND-1000 spectrophotometer (Nanodrop Technologies; Thermo Fisher Scientific, Inc., Wilmington, DE, USA) and qualitatively analyzed with an RNA 6000 LabChip kit (Agilent Technologies, Inc., Santa Clara, CA, USA) and the Bioanalyzer 2100 (Agilent Technologies, Inc.). .. Library preparation and deep sequencing were performed according to the manufacturer's protocol (Illumina, Welgene Biotechnology Company, Taipei, Taiwan), as described in our previous reports ( , ).

Article Title: The first whole genome and transcriptome of the cinereous vulture reveals adaptation in the gastric and immune defense systems and possible convergent evolution between the Old and New World vultures
Article Snippet: RNA quality was assessed by analysis of rRNA band integrity on an Agilent RNA 6000 LabChip kit (Agilent Technologies). .. Prior to cDNA library construction, 2 μg of total RNA was treated with DNase I, and magnetic beads with Oligo (dT) were used to enrich poly (A) mRNA.

Reverse Transcription Polymerase Chain Reaction:

Article Title: Transcriptome Analysis of PPAR? Target Genes Reveals the Involvement of Lysyl Oxidase in Human Placental Cytotrophoblast Invasion
Article Snippet: RNA was extracted using the TRIzol® reagent (Invitrogen) as described for RT-PCR, and then purified using the RNeasy® Mini kit (Quiagen S.A., Courtabœuf, France). .. RNA integrity and purity were checked using a 2100 Bioanalyzer with the RNA 6000 LabChip kit (Agilent Technologies, Massy, France).

Article Title: The Serotonin Receptor Subtype 5b Specifically Interacts with Serotonin Receptor Subtype 1A
Article Snippet: Paragraph title: RT-PCR ... The total RNA of homogenized tissue from specific brain regions was isolated using the Trizol® method according to manufacturer’s instructions (GibcoBRL) and its concentration was determined using the nanodrop ND-1000 spectrophotometer followed by its quality and integrity measurement by electrophoresis on RNA 6000 LabChip® kit (Agilent 2100 Bioanalyzer).

Quantitative RT-PCR:

Article Title: Gene Network Analysis of Glucose Linked Signaling Pathways and Their Role in Human Hepatocellular Carcinoma Cell Growth and Survival in HuH7 and HepG2 Cell Lines
Article Snippet: Paragraph title: 2.4. Messenger RNA Quantification by Real-Time Quantitative PCR (RT-qPCR) ... RNA integrity was assessed with the Agilent 2100 Bioanalyzer and RNA 6000 LabChip Kit (Agilent Technologies, Massy, France).

Article Title: White Adipose Tissue Resilience to Insulin Deprivation and Replacement
Article Snippet: Paragraph title: mRNA quantification by qRT PCR ... RNA integrity was assessed with the Agilent 2100 Bioanalyzer and RNA 6000 LabChip Kit (Agilent Technologies, Massy, France).

Article Title: The Serotonin Receptor Subtype 5b Specifically Interacts with Serotonin Receptor Subtype 1A
Article Snippet: The total RNA of homogenized tissue from specific brain regions was isolated using the Trizol® method according to manufacturer’s instructions (GibcoBRL) and its concentration was determined using the nanodrop ND-1000 spectrophotometer followed by its quality and integrity measurement by electrophoresis on RNA 6000 LabChip® kit (Agilent 2100 Bioanalyzer). .. The total RNA of homogenized tissue from specific brain regions was isolated using the Trizol® method according to manufacturer’s instructions (GibcoBRL) and its concentration was determined using the nanodrop ND-1000 spectrophotometer followed by its quality and integrity measurement by electrophoresis on RNA 6000 LabChip® kit (Agilent 2100 Bioanalyzer).

Article Title: Analysis of the Serotonergic System in a Mouse Model of Rett Syndrome Reveals Unusual Upregulation of Serotonin Receptor 5b
Article Snippet: Paragraph title: Quantitative RT-PCR ... The total ribonucleic acid (RNA) of homogenized brain tissue (hippocampus and brainstem) was isolated using the Trizol® method according to manufacturer’s instructions (GibcoBRL) and its concentration was determined using the nanodrop ND-1000 spectrophotometer followed by its quality and integrity measurement by electrophoresis on RNA 6000 LabChip® kit (Agilent 2100 Bioanalyzer).

Sample Prep:

Article Title: The first whole genome and transcriptome of the cinereous vulture reveals adaptation in the gastric and immune defense systems and possible convergent evolution between the Old and New World vultures
Article Snippet: Paragraph title: Sample preparation and sequencing ... RNA quality was assessed by analysis of rRNA band integrity on an Agilent RNA 6000 LabChip kit (Agilent Technologies).

Mouse Assay:

Article Title: A 40 kDa protein of the inner membrane is the mitochondrial calcium uniporter
Article Snippet: For the expression analysis of MCU and MICU1 in mouse tissues, adult male C57BL/6 mice (28-30 g) were used. .. The RNA was quantified and controlled for its quality using the RNA 6000 LabChip kit (Agilent Technologies) in conjunction with an Agilent Bioanalyzer 2001.

In Situ Hybridization:

Article Title: Systems analysis identifies melanoma-enriched pro-oncogenic networks controlled by the RNA binding protein CELF1
Article Snippet: Quality assessment of Total RNA samples were analyzed using the Agilent Bioanalyzer 2100 and the RNA 6000 LabChip Kit (Agilent) with the Eukaryote Total RNA Nano Assay (Agilent). .. Quality assessment of Total RNA samples were analyzed using the Agilent Bioanalyzer 2100 and the RNA 6000 LabChip Kit (Agilent) with the Eukaryote Total RNA Nano Assay (Agilent).

Software:

Article Title: Transcriptome Analysis of PPAR? Target Genes Reveals the Involvement of Lysyl Oxidase in Human Placental Cytotrophoblast Invasion
Article Snippet: RNA integrity and purity were checked using a 2100 Bioanalyzer with the RNA 6000 LabChip kit (Agilent Technologies, Massy, France). .. Data were processed with the Expression Analysis algorithm of the Affymetrix Microarray suite (version 4.0).

Article Title: Safety of Bifidobacterium animalis subsp. lactis (B. lactis) strain BB-12-supplemented yogurt in healthy adults on antibiotics: a phase I safety study
Article Snippet: DNAse-treated RNA was quantified using the Agilent Bioanalyzer 2100 and RNA 6000 Labchip Kit (Agilent Technologies, Palo Alto, CA). .. Briefly, cDNA was synthesized with Superscript RT (Invitrogen) and oligo (dT), and 50 ng of this cDNA was used for real-time PCR amplifications using a Thermo-Start DNA Polymerase master mix (Abgene, Rochester, NY) and the ABI PRISM 7700 Sequence detector system (Applied Biosystems, Foster City, CA).

Article Title: Deduction of novel genes potentially involved in hypoxic AC16 human cardiomyocytes using next-generation sequencing and bioinformatics approaches
Article Snippet: The purified RNA was measured at OD260nm with an ND-1000 spectrophotometer (Nanodrop Technologies; Thermo Fisher Scientific, Inc., Wilmington, DE, USA) and qualitatively analyzed with an RNA 6000 LabChip kit (Agilent Technologies, Inc., Santa Clara, CA, USA) and the Bioanalyzer 2100 (Agilent Technologies, Inc.). .. For small RNA sequencing, following reverse transcription of total RNA, cDNA with sizes indicating 18-40-nucleotide RNA fragments (140-155 nucleotides in length with both adapters) was extracted and sequenced on the Illumina instrument (75 single-end cycles).

SYBR Green Assay:

Article Title: Gene Network Analysis of Glucose Linked Signaling Pathways and Their Role in Human Hepatocellular Carcinoma Cell Growth and Survival in HuH7 and HepG2 Cell Lines
Article Snippet: RNA integrity was assessed with the Agilent 2100 Bioanalyzer and RNA 6000 LabChip Kit (Agilent Technologies, Massy, France). .. First strand cDNAs were synthesized from 500 ng of total RNA in the presence of 100 U of Superscript (Invitrogen-Life Technologies, Eragny, France) and random hexamers and oligo-dT primers (Promega).

Article Title: White Adipose Tissue Resilience to Insulin Deprivation and Replacement
Article Snippet: RNA integrity was assessed with the Agilent 2100 Bioanalyzer and RNA 6000 LabChip Kit (Agilent Technologies, Massy, France). .. First strand cDNAs were synthesized from 1 µg of total RNA in the presence of 100 U of Superscript II (Invitrogen-Life Technologies, Eragny, France) and random hexamers and oligo(dT) primers (Promega, Charbonnières, France).

RNA Extraction:

Article Title: Evaluation of two commercial and three home-made fixatives for the substitution of formalin: a formaldehyde-free laboratory is possible
Article Snippet: Paragraph title: RNA extraction ... RNA samples were also tested for their quality using the RNA 6000 LabChip kit (Agilent Technologies) following the manufacturer’s instructions, and RNA analysis was evaluated with the 2100 Bioanalyzer (Agilent Technologies).

Article Title: A 40 kDa protein of the inner membrane is the mitochondrial calcium uniporter
Article Snippet: Paragraph title: RNA extraction and Gene Expression Analyses ... The RNA was quantified and controlled for its quality using the RNA 6000 LabChip kit (Agilent Technologies) in conjunction with an Agilent Bioanalyzer 2001.

Article Title: Allele-specific expression in a family quartet with autism reveals mono-to-biallelic switch and novel transcriptional processes of autism susceptibility genes
Article Snippet: Paragraph title: RNA extraction and RNA sequencing (RNA-Seq) ... The quality of RNA was based on the RNA integrity number (RIN) measured with a Bioanalyzer 2100 (Agilent Technology) and an RNA 6000 LabChip Kit (Agilent Technology).

Agarose Gel Electrophoresis:

Article Title: The first whole genome and transcriptome of the cinereous vulture reveals adaptation in the gastric and immune defense systems and possible convergent evolution between the Old and New World vultures
Article Snippet: RNA quality was assessed by analysis of rRNA band integrity on an Agilent RNA 6000 LabChip kit (Agilent Technologies). .. The cDNAs were subjected to end-repair and poly (A) addition, and then connected with sequencing adapters using the TruSeq RNA Sample Prep Kit (Illumina).

Electrophoresis:

Article Title: The Serotonin Receptor Subtype 5b Specifically Interacts with Serotonin Receptor Subtype 1A
Article Snippet: The primers used for generation of different variants of Htr1a -Htr5b hybrid receptor variants are given in Table . .. The total RNA of homogenized tissue from specific brain regions was isolated using the Trizol® method according to manufacturer’s instructions (GibcoBRL) and its concentration was determined using the nanodrop ND-1000 spectrophotometer followed by its quality and integrity measurement by electrophoresis on RNA 6000 LabChip® kit (Agilent 2100 Bioanalyzer). .. The RNA was transcribed into the cDNA using the iScript cDNA Synthesis Kit (BioRad).

Article Title: Analysis of the Serotonergic System in a Mouse Model of Rett Syndrome Reveals Unusual Upregulation of Serotonin Receptor 5b
Article Snippet: Gene expression was analyzed by quantitative RT-PCR analysis. .. The total ribonucleic acid (RNA) of homogenized brain tissue (hippocampus and brainstem) was isolated using the Trizol® method according to manufacturer’s instructions (GibcoBRL) and its concentration was determined using the nanodrop ND-1000 spectrophotometer followed by its quality and integrity measurement by electrophoresis on RNA 6000 LabChip® kit (Agilent 2100 Bioanalyzer). .. The RNA was transcribed into the corresponding deoxyribonucleic acid (cDNA) using the iScript cDNA Synthesis Kit (BioRad).

Homogenization:

Article Title: Microarray analysis of subcutaneous adipose tissue from mature cows with divergent body weight gain after feed restriction and realimentation
Article Snippet: 2.3 Total RNA was extracted from 50 to 100 mg of tissue by homogenization with TriPure reagent (Roche, Indianapolis, IN) following the manufacturer's protocol with the exception of an increased time of centrifugation from 15 to 20 min following the addition of chloroform. .. The quality of total RNA was determined by running samples on a RNA 6000 LabChip kit (Agilent Technologies, Santa Clara, CA) with the Agilent 2100 bioanalyzer.

Ethanol Precipitation:

Article Title: IGF1R activation and the in vitro antiproliferative efficacy of IGF1R inhibitor are inversely correlated with IGFBP5 expression in bladder cancer
Article Snippet: The RNA and DNA were further purified by phenol–chloroform extraction and ethanol precipitation, and the proteins were dialyzed and lyophilized. .. The concentration, integrity and purity of each RNA sample were determined with the RNA 6000 LabChip Kit (Agilent Technologies, Massy, France) and an Agilent 2100 bioanalyzer.

dsDNA Assay:

Article Title: The first whole genome and transcriptome of the cinereous vulture reveals adaptation in the gastric and immune defense systems and possible convergent evolution between the Old and New World vultures
Article Snippet: The amount of DNA was quantified by fluorometry using a Qubit dsDNA Assay Kit (Invitrogen, CA, USA) and an Infinite F200 Pro NanoQuant (TECAN, Männedorf, Germany). .. RNA quality was assessed by analysis of rRNA band integrity on an Agilent RNA 6000 LabChip kit (Agilent Technologies).

Spectrophotometry:

Article Title: Evaluation of two commercial and three home-made fixatives for the substitution of formalin: a formaldehyde-free laboratory is possible
Article Snippet: RNA concentration was determined at 260 nm and 280 nm with a Thermo Scientific Nanodrop 2000 spectrophotometer (EuroClone, Italy). .. RNA samples were also tested for their quality using the RNA 6000 LabChip kit (Agilent Technologies) following the manufacturer’s instructions, and RNA analysis was evaluated with the 2100 Bioanalyzer (Agilent Technologies).

Article Title: An integrative genomic analysis revealed the relevance of microRNA and gene expression for drug-resistance in human breast cancer cells
Article Snippet: The yield and purity of the genomic DNA and total RNA were measured using a NanoDrop ND-1000 spectrophotometer (Thermo Fisher Scientific). .. The quality of the total RNA was verified to have an RNA Integrity Number using a Bioanalyzer and RNA 6000 LabChip Kit (Agilent Technologies).

Article Title: Allele-specific expression in a family quartet with autism reveals mono-to-biallelic switch and novel transcriptional processes of autism susceptibility genes
Article Snippet: The RNA was quantified with an ND-1000 spectrophotometer (Nanodrop Technology). .. The quality of RNA was based on the RNA integrity number (RIN) measured with a Bioanalyzer 2100 (Agilent Technology) and an RNA 6000 LabChip Kit (Agilent Technology).

Article Title: The Serotonin Receptor Subtype 5b Specifically Interacts with Serotonin Receptor Subtype 1A
Article Snippet: The primers used for generation of different variants of Htr1a -Htr5b hybrid receptor variants are given in Table . .. The total RNA of homogenized tissue from specific brain regions was isolated using the Trizol® method according to manufacturer’s instructions (GibcoBRL) and its concentration was determined using the nanodrop ND-1000 spectrophotometer followed by its quality and integrity measurement by electrophoresis on RNA 6000 LabChip® kit (Agilent 2100 Bioanalyzer). .. The RNA was transcribed into the cDNA using the iScript cDNA Synthesis Kit (BioRad).

Article Title: Analysis of the Serotonergic System in a Mouse Model of Rett Syndrome Reveals Unusual Upregulation of Serotonin Receptor 5b
Article Snippet: Gene expression was analyzed by quantitative RT-PCR analysis. .. The total ribonucleic acid (RNA) of homogenized brain tissue (hippocampus and brainstem) was isolated using the Trizol® method according to manufacturer’s instructions (GibcoBRL) and its concentration was determined using the nanodrop ND-1000 spectrophotometer followed by its quality and integrity measurement by electrophoresis on RNA 6000 LabChip® kit (Agilent 2100 Bioanalyzer). .. The RNA was transcribed into the corresponding deoxyribonucleic acid (cDNA) using the iScript cDNA Synthesis Kit (BioRad).

Article Title: Microarray analysis of subcutaneous adipose tissue from mature cows with divergent body weight gain after feed restriction and realimentation
Article Snippet: Purified RNA was quantified using a NanoDrop 8000 spectrophotometer (Thermo Scientific, Wilmington, DE). .. The quality of total RNA was determined by running samples on a RNA 6000 LabChip kit (Agilent Technologies, Santa Clara, CA) with the Agilent 2100 bioanalyzer.

Article Title: Deduction of novel genes potentially involved in hypoxic AC16 human cardiomyocytes using next-generation sequencing and bioinformatics approaches
Article Snippet: Total RNAs from the normoxic and hypoxic AC16 CMs were extracted with TRIzol reagent (Invitrogen, Thermo Fisher Scientific, Inc.) following the manufacturer's standard protocol. .. The purified RNA was measured at OD260nm with an ND-1000 spectrophotometer (Nanodrop Technologies; Thermo Fisher Scientific, Inc., Wilmington, DE, USA) and qualitatively analyzed with an RNA 6000 LabChip kit (Agilent Technologies, Inc., Santa Clara, CA, USA) and the Bioanalyzer 2100 (Agilent Technologies, Inc.). .. Library preparation and deep sequencing were performed according to the manufacturer's protocol (Illumina, Welgene Biotechnology Company, Taipei, Taiwan), as described in our previous reports ( , ).

Activation Assay:

Article Title: Safety of Bifidobacterium animalis subsp. lactis (B. lactis) strain BB-12-supplemented yogurt in healthy adults on antibiotics: a phase I safety study
Article Snippet: The mRNA expression level of selected genes associated with transcription factors: T-box transcription factor ( TBX21 ), GATA binding protein 3 ( GATA 3 ), Forkhead box P3 ( FOXP3 ), Interferon regulatory factor ( IRF8 ); Toll like receptors TLR2 , TLR4 and TLR9 ; pro-inflammatory and regulatory cytokines: Tumor necrosis factor α ( TNFA ), Interleukin 6 ( IL6 ), Interleukin 1b ( IL1B ), Tumor necrosis factor ligand superfamily member 14 ( TNFSF14 ), interferon gamma ( IFNg ), Tumor growth factor β-2 ( TGFB2 ), Interleukin 10 ( IL10 ); cell activation markers: CD40, HLA-DRA, CD80, CD86, CD274 ; and chemokines CXCL10, CCL3, CCL4 was measured on cDNA synthesized from each sample using 1 μg of total RNA. .. DNAse-treated RNA was quantified using the Agilent Bioanalyzer 2100 and RNA 6000 Labchip Kit (Agilent Technologies, Palo Alto, CA).

Fractionation:

Article Title: Population and individual-specific regulatory variation in Sardinia
Article Snippet: For each participant, leukocytes were isolated from whole blood using the LeukoLOCK™ fractionation kit and RNA was extracted using TRI Reagent® (Ambion #AM9738) and isolated using the PureLink® RNA Mini Kit (Ambion #12183018A). .. The quantity and the integrity of isolated RNA samples was evaluated using the Agilent Technologies 2100 Bioanalyzer platform with the RNA 6000 LabChip® kit (Agilent #5067-1511) - samples with an RNA integrity number (RIN) less than 7.5 were discarded.

Standard Deviation:

Article Title: Safety of Bifidobacterium animalis subsp. lactis (B. lactis) strain BB-12-supplemented yogurt in healthy adults on antibiotics: a phase I safety study
Article Snippet: DNAse-treated RNA was quantified using the Agilent Bioanalyzer 2100 and RNA 6000 Labchip Kit (Agilent Technologies, Palo Alto, CA). .. The sequence information for human genes assayed can be found in the Porcine Translational Research Database from the Diet, Genomics, and Immunology Laboratory, Beltsville Human Nutrition Research Center, Agricultural Research Service-United States Department of Agriculture ( ).

Staining:

Article Title: Characterisation of a Novel Fc Conjugate of Macrophage Colony-stimulating Factor
Article Snippet: RNA integrity and quality was assessed using Following the RNA 6000 LabChip Kit (Agilent). .. RNA integrity and quality was assessed using Following the RNA 6000 LabChip Kit (Agilent).

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  • 99
    Agilent technologies rna 6000 nano labchip kit
    Extracting High Quality RNA from Bone in a Single Step . (A) Isolated liver RNA was incubated with RNase free beads (lane 1) or untreated beads (lane 2) provided by the manufacturer (Next Advance) for four hours before analyzing the 18S and 28S rRNA bands using agarose gel chromatography. Subsequent experiments with the Bullet Blender were carried out using untreated beads. (B) Bone RNA was homogenized in near freezing conditions using the Bullet Blender centrifuge (lanes 2-4) or a Polytron (lanes 5-6). The results are compared to bone RNA isolated using standard homogenization conditions (lane 1) and intact liver RNA that was previously isolated (control). (C) The RNA Integrity Number (RIN) for RNA homogenized in near freezing conditions was determined using the Agilent RNA 6000 <t>Nano</t> <t>LabChip</t> Kit and the Agilent 2100 Bioanalyzer. RNA with a RIN = 7 or greater is suitable for microarray analysis of gene expression. (D) The maximum RIN for the two-step approach (lane 2) is compared to the maximum RIN obtained in the one-step approach (lane 3) and each RIN is compared to the RIN of the degraded RNA sample (lane 1) shown in the agarose gel in lane 1, Figure 2B. (E) The electropherograms associated with the samples shown in (D).
    Rna 6000 Nano Labchip Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 123 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rna 6000 nano labchip kit/product/Agilent technologies
    Average 99 stars, based on 123 article reviews
    Price from $9.99 to $1999.99
    rna 6000 nano labchip kit - by Bioz Stars, 2019-12
    99/100 stars
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    97
    Agilent technologies rna 6000 pico labchip kit
    Integrity of the RNA samples. Each bar depicts the arithmetic mean of the RNA integrity numbers (RIN) measured for the 10 whole RNA samples, which were used for the microarray hybridisation per cell population and per time point. The error bars indicate standard deviations. This quality control was carried out using the Agilent Bioanalyzer 2100 and the RNA 6000 Pico <t>LabChip</t> kit (Agilent Technologies, Waldbronn, Germany).
    Rna 6000 Pico Labchip Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 97/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rna 6000 pico labchip kit/product/Agilent technologies
    Average 97 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    rna 6000 pico labchip kit - by Bioz Stars, 2019-12
    97/100 stars
      Buy from Supplier

    Image Search Results


    Extracting High Quality RNA from Bone in a Single Step . (A) Isolated liver RNA was incubated with RNase free beads (lane 1) or untreated beads (lane 2) provided by the manufacturer (Next Advance) for four hours before analyzing the 18S and 28S rRNA bands using agarose gel chromatography. Subsequent experiments with the Bullet Blender were carried out using untreated beads. (B) Bone RNA was homogenized in near freezing conditions using the Bullet Blender centrifuge (lanes 2-4) or a Polytron (lanes 5-6). The results are compared to bone RNA isolated using standard homogenization conditions (lane 1) and intact liver RNA that was previously isolated (control). (C) The RNA Integrity Number (RIN) for RNA homogenized in near freezing conditions was determined using the Agilent RNA 6000 Nano LabChip Kit and the Agilent 2100 Bioanalyzer. RNA with a RIN = 7 or greater is suitable for microarray analysis of gene expression. (D) The maximum RIN for the two-step approach (lane 2) is compared to the maximum RIN obtained in the one-step approach (lane 3) and each RIN is compared to the RIN of the degraded RNA sample (lane 1) shown in the agarose gel in lane 1, Figure 2B. (E) The electropherograms associated with the samples shown in (D).

    Journal: BMC Biotechnology

    Article Title: An improved method for isolation of RNA from bone

    doi: 10.1186/1472-6750-12-5

    Figure Lengend Snippet: Extracting High Quality RNA from Bone in a Single Step . (A) Isolated liver RNA was incubated with RNase free beads (lane 1) or untreated beads (lane 2) provided by the manufacturer (Next Advance) for four hours before analyzing the 18S and 28S rRNA bands using agarose gel chromatography. Subsequent experiments with the Bullet Blender were carried out using untreated beads. (B) Bone RNA was homogenized in near freezing conditions using the Bullet Blender centrifuge (lanes 2-4) or a Polytron (lanes 5-6). The results are compared to bone RNA isolated using standard homogenization conditions (lane 1) and intact liver RNA that was previously isolated (control). (C) The RNA Integrity Number (RIN) for RNA homogenized in near freezing conditions was determined using the Agilent RNA 6000 Nano LabChip Kit and the Agilent 2100 Bioanalyzer. RNA with a RIN = 7 or greater is suitable for microarray analysis of gene expression. (D) The maximum RIN for the two-step approach (lane 2) is compared to the maximum RIN obtained in the one-step approach (lane 3) and each RIN is compared to the RIN of the degraded RNA sample (lane 1) shown in the agarose gel in lane 1, Figure 2B. (E) The electropherograms associated with the samples shown in (D).

    Article Snippet: RNA yield was determined by the absorbance at 260 nm (Table ) and using the Agilent RNA 6000 Nano LabChip Kit and the Agilent 2100 Bioanalyzer (Agilent Technologies).

    Techniques: Isolation, Incubation, Agarose Gel Electrophoresis, Chromatography, Homogenization, Microarray, Expressing

    Analysis of IL-6 mRNA levels within synovial tissue from rheumatoid arthritis (RA) as compared with that from osteoarthritis (OA) patients. Upper panels: quality control of total RNA preparations. Aliquots (300 ng) of total RNA extracted from synovial tissue from RA and OA patients were plotted on a RNA 6000 Nano-LabChip. Quality of RNA was scanned using a 2100 bioanalyzer. RNA gel electropherograms show the presence of 28S and 18S ribosomal units, indicating intact RNA of the investigated samples. Lower panels: differential IL-6 mRNA levels were determined by semiquantitative reverse transcription polymerase chain reaction (PCR). The figure shows a representative analysis of eight cDNA samples derived from patients with RA and of eight cDNA samples from patients with OA. cDNA samples were adjusted to equal glyceraldehyde-3-phosphate dehydrogenase (G3PDH) levels, performed by competitive PCR using an internal standard (see Materials and methods). Numbered lanes correspond to individual patients within Table 1 .

    Journal: Arthritis Research & Therapy

    Article Title: High CXCR3 expression in synovial mast cells associated with CXCL9 and CXCL10 expression in inflammatory synovial tissues of patients with rheumatoid arthritis

    doi:

    Figure Lengend Snippet: Analysis of IL-6 mRNA levels within synovial tissue from rheumatoid arthritis (RA) as compared with that from osteoarthritis (OA) patients. Upper panels: quality control of total RNA preparations. Aliquots (300 ng) of total RNA extracted from synovial tissue from RA and OA patients were plotted on a RNA 6000 Nano-LabChip. Quality of RNA was scanned using a 2100 bioanalyzer. RNA gel electropherograms show the presence of 28S and 18S ribosomal units, indicating intact RNA of the investigated samples. Lower panels: differential IL-6 mRNA levels were determined by semiquantitative reverse transcription polymerase chain reaction (PCR). The figure shows a representative analysis of eight cDNA samples derived from patients with RA and of eight cDNA samples from patients with OA. cDNA samples were adjusted to equal glyceraldehyde-3-phosphate dehydrogenase (G3PDH) levels, performed by competitive PCR using an internal standard (see Materials and methods). Numbered lanes correspond to individual patients within Table 1 .

    Article Snippet: Quality of all total RNA samples was controlled by a 2100 bioanalyzer according to a RNA 6000 Nano-LabChip Kit procedure (Agilent Technologies, Palo Alto, CA, USA), using 0.3 μg of each total RNA. cDNA was synthesized from 1 μg total RNA in a 20 μl reaction using 200 U Superscript™ II reverse transcriptase (Life Technologies), 500 μmol/l of each deoxynucleotide, 5 mmol/l DTT and 0.5 μg of oligo(dT)15 (Invitek, Berlin, Germany).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Polymerase Chain Reaction, Derivative Assay

    Integrity of the RNA samples. Each bar depicts the arithmetic mean of the RNA integrity numbers (RIN) measured for the 10 whole RNA samples, which were used for the microarray hybridisation per cell population and per time point. The error bars indicate standard deviations. This quality control was carried out using the Agilent Bioanalyzer 2100 and the RNA 6000 Pico LabChip kit (Agilent Technologies, Waldbronn, Germany).

    Journal: Scientific Data

    Article Title: Microarray data of transcriptome shifts in blood cell subsets during S1P receptor modulator therapy

    doi: 10.1038/sdata.2018.145

    Figure Lengend Snippet: Integrity of the RNA samples. Each bar depicts the arithmetic mean of the RNA integrity numbers (RIN) measured for the 10 whole RNA samples, which were used for the microarray hybridisation per cell population and per time point. The error bars indicate standard deviations. This quality control was carried out using the Agilent Bioanalyzer 2100 and the RNA 6000 Pico LabChip kit (Agilent Technologies, Waldbronn, Germany).

    Article Snippet: The integrity of the RNA samples was checked using an Agilent Bioanalyzer 2100 with the RNA 6000 Pico LabChip kit (both from Agilent Technologies, Waldbronn, Germany).

    Techniques: Microarray, Hybridization