ricinus communis agglutinin 1 rca 1 lectins  (Vector Laboratories)

Journal: Journal of Neuroinflammation

Article Title: Acute paretic syndrome in juvenile White Leghorn chickens resembles late stages of acute inflammatory demyelinating polyneuropathies in humans

doi: 10.1186/1742-2094-7-7

Figure Lengend Snippet: Immunohistochemical illustration of Ricinus communis agglutinin-1 (RCA-1) -positive microglial cells in the white matter of spinal cord . The RCA-1-positive microglial cells (arrowheads) in both healthy (A) and AvIDP-affected (B) chicken show incospicuous ramified microglial cells. Activated microglial cells or an increased number were not observed. A, B: asterisk = endothelium (also stains RCA-1 positive); scale bar = 250 μm.

Article Snippet: Furthermore, the biotinylated lectin Ricinus communis agglutinin-1 (RCA 120, 1:3000; Vector Laboratories, CA, USA) was used as a specific marker for microglial cells within the spinal cord [ ].

Techniques: Immunohistochemical staining

Journal: Pharmaceuticals

Article Title: Regenerative Effects of CDP-Choline: A Dose-Dependent Study in the Toxic Cuprizone Model of De- and Remyelination

doi: 10.3390/ph14111156

Figure Lengend Snippet: Graphs represent the qualitative analysis (cells/mm 2 ) of activated microglia (( A )—RCA-1 positive microglia; ( B )—Mac-3 positive microglia, ( C )—proliferating RCA-1/Ki-67 positive microglia), and GFAP positive astrocytes ( D ) during early remyelination (week 5.5) in the corpus callosum of animals treated with different dosages of CDP-choline or sham (PBS). The astrogliosis was not affected by CDP-choline treatment. However, the number of activated microglia in all animals treated with CDP-choline, regardless of the dose used, was significantly lower than in the control group. Significant effects between mice treated with different dosages of CDP-choline and PBS are indicated by asterisks (* p < 0.05, ** p < 0.01, *** p < 0.001, n = 6).

Article Snippet: The following primary antibodies were used: for myelin proteolipid protein (PLP) (mouse monoclonal IgG2a, 1:500, Serotec) and myelin basic protein (MBP) (mouse monoclonal IgG2b, 1:500, Covance), for activated microglia Mac-3 (rat IgG, 1:500, BD Pharmingen) and the lectin ricinus communis agglutinin 1 (RCA-1) (1:1000, biotinylated, Vector Laboratories), for astrocytes glial fibrillary acidic protein (GFAP) (mouse IgG, 1:200, Millipore), for oligodendrocytes Nogo-A (rabbit IgG, 1:750, Millipore) and anti-adenomatus polyposis coli (APC; mouse IgG; 1:200).

Techniques:

Journal: STAR Protocols

Article Title: Protocol for induction and characterization of microglia extracellular traps in murine and human microglia cells

doi: 10.1016/j.xpro.2021.100678

Figure Lengend Snippet:

Article Snippet: Fluorescein Ricinus communis agglutinin-1 lectin , Vector Labs , FL-1081.

Techniques: Recombinant, Staining, Plasmid Preparation, Software, Phagocytosis Assay, Microscopy

Journal: Scientific Reports

Article Title: Differential Expression Profile of NLRs and AIM2 in Glioma and Implications for NLRP12 in Glioblastoma

doi: 10.1038/s41598-019-44854-4

Figure Lengend Snippet: NLRP12 inhibition regulates cellular proliferation. ( a ) NLRP12 expression was observed in glioma (LN18) and microglial (BV2) cells using immunofluorescence (magnification: 40X, scale bar: 50 μm). ( b ) Colony formation assay shows increased cellular proliferation in the NLRP12si treated microglia. Magnification: 40X. ( c , d ) Quantification of both BV2 and LN18 cells per colony. ( e ) Effect of LN18 (NLRP12 siRNA treated) cell conditioned medium (CM) on BV2 cell migration was assessed using migration assay. 8 image sections per sample were quantified for migration analysis. Student’s t-test and one-way AVOVA were performed to find the statistical significance of colony formation and migration assay respectively (p-value; *<0.05, **<0.005). The error bar indicates standard error of mean. ( f ) NLRP12 expression in human normal brain tissue and glioma sections, using anti-NLRP12 antibody (N = 7). Nuclei were stained blue with DAPI and microglia were stained green with FITC tagged lectin RCA (Ricinus Communis Agglutinin-1); Magnification: 20X, scale bar: 100 µm.

Article Snippet: For the detection of microglia, tissues were stained with Ricinus communis agglutinin-1 (RCA-1) lectin (Vector labs, FL-1081) .

Techniques: Inhibition, Expressing, Immunofluorescence, Colony Assay, Migration, Staining