ribopure yeast kit  (Thermo Fisher)


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    Structured Review

    Thermo Fisher ribopure yeast kit
    Ribopure Yeast Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ribopure yeast kit/product/Thermo Fisher
    Average 97 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    ribopure yeast kit - by Bioz Stars, 2020-04
    97/100 stars

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    Related Articles

    Centrifugation:

    Article Title: mRNA stability changes precede changes in steady-state mRNA amounts during hyperosmotic stress
    Article Snippet: From transcriptionally inhibited samples, 2.5 mL aliquots were harvested by centrifugation and washing (twice) at 5, 15, 30, and 60 min (the 5 min time point was not included in the subsequent analysis due to the high variability of the measurements). .. Cells were disrupted and RNA extracted using the RiboPure-yeast kit and instructions from the supplier (Applied Biosystems/Ambion).

    Article Title: Intra and Interspecific Variations of Gene Expression Levels in Yeast Are Largely Neutral: (Nei Lecture, SMBE 2016, Gold Coast)
    Article Snippet: Cultures were grown at 30 °C until OD660 = 0.5 (mid-log phase), at which point cells were harvested by centrifugation. .. Briefly, total RNA was extracted from each population using RiboPure-Yeast Kit (Ambion) and treated with DNase I to remove any contaminant DNA.

    Article Title: Transcriptional responses of Candida glabrata biofilm cells to fluconazole are modulated by the carbon source
    Article Snippet: Then biofilms were scraped from the plates with 500 µL of PBS per well, sonicated for 30 s at 30% amplitude (Ultrasonic Processor, Cole-Parmer, IL, USA) in order to separate cells from matrix and then harvested by centrifugation at 8000 × g for 10 min at 4 °C. .. Cells drops were flash frozen in liquid nitrogen and total RNA was isolated from frozen cell pellets using the RiboPure Yeast kit (Ambion, Life Technologies, USA), according to manufacturer’s instructions.

    Article Title: The GRF10 homeobox gene regulates filamentous growth in the human fungal pathogen Candida albicans
    Article Snippet: Cells were quickly cooling on ice and pelleted by centrifugation at 4°C for 5 min; the cell pellet was frozen at −80°C. .. RNA was extracted from the frozen pellets using the RiboPure Yeast Kit (Ambion) following the manufacturer's instructions, except that less RNA (3 μg) was treated with DNaseI. cDNA was synthesized from 200 ng of RNA using the SensiFAST cDNA synthesis kit (Bioline), following the manufacturer's instructions.

    Synthesized:

    Article Title: Synergetic effect of yeast cell-surface expression of cellulase and expansin-like protein on direct ethanol production from cellulose
    Article Snippet: Total RNA was isolated from yeast cells cultivated in YPD medium for 48 h at 30°C using a RiboPure Yeast Kit (Ambion, Austin, TX, USA), and was then used for cDNA synthesis using a ReverTra Ace qPCR RT Kit (Toyobo). .. The synthesized cDNA was used as a template for real-time RT-PCR, which was performed with three sets of PCR primers: (BGL761(F) and BGL858(R), EGII968(F) and EGII1043(R), CBHII387(F) and CBHII455(R), and AoelpI567(F) and AoelpI637(R)) (Table ).

    Article Title: The GRF10 homeobox gene regulates filamentous growth in the human fungal pathogen Candida albicans
    Article Snippet: .. RNA was extracted from the frozen pellets using the RiboPure Yeast Kit (Ambion) following the manufacturer's instructions, except that less RNA (3 μg) was treated with DNaseI. cDNA was synthesized from 200 ng of RNA using the SensiFAST cDNA synthesis kit (Bioline), following the manufacturer's instructions. .. The synthesized cDNA was diluted 1:5 in DEPC-treated water.

    Quantitative RT-PCR:

    Article Title: A Multistate Toggle Switch Defines Fungal Cell Fates and Is Regulated by Synergistic Genetic Cues
    Article Snippet: Cells were removed from the medium and RNA isolated using the Ribopure-Yeast Kit (Ambion). .. RNA was treated with Turbo DNase (Ambion), and 500 ng of RNA used for cDNA generation using the GoScript enzyme (Promega). qRT-PCR was then performed by using primers in . cDNA levels were normalized to ACT1 expression levels.

    Article Title: Synergetic effect of yeast cell-surface expression of cellulase and expansin-like protein on direct ethanol production from cellulose
    Article Snippet: Paragraph title: Quantification of cellulase-encoding genes transcription by real-time RT-PCR ... Total RNA was isolated from yeast cells cultivated in YPD medium for 48 h at 30°C using a RiboPure Yeast Kit (Ambion, Austin, TX, USA), and was then used for cDNA synthesis using a ReverTra Ace qPCR RT Kit (Toyobo).

    Article Title: Comparison of the efficiency of different cell lysis methods and different commercial methods for RNA extraction from Candida albicans stored in RNAlater
    Article Snippet: .. Hence, although all extraction methods succeeded to yield RNA after storage in TSB-G and RNAlater, the RiboPure Yeast Kit resulted in (mostly significantly) higher yields (as measured with NanoDrop, Fragment Analyzer, or RT-qPCR) compared to both other methods. .. However, when RNA integrity is considered, RNeasy Mini Kit and easyMAG showed better quality in samples stored in RNAlater.

    Article Title: The GRF10 homeobox gene regulates filamentous growth in the human fungal pathogen Candida albicans
    Article Snippet: RNA was extracted from the frozen pellets using the RiboPure Yeast Kit (Ambion) following the manufacturer's instructions, except that less RNA (3 μg) was treated with DNaseI. cDNA was synthesized from 200 ng of RNA using the SensiFAST cDNA synthesis kit (Bioline), following the manufacturer's instructions. .. The RT-qPCR was performed on each cDNA sample following the manufacturer's instructions (SensiFAST SYBR No-ROX Kit, Bioline) using primers to GRF10 (forward 5′ -CAAACCGCTCAAATAGTCAAAG-3′ and reverse 5′ -GCAAATTGTGGAGAGTTTGTAG-3′ ) and the reference gene TEF1 (forward 5′ -TTCGTCAAATCCGGTGATG-3′ and reverse 5′ -CTGACAGCGAATCTACCTAATG-3′ ).

    Real-time Polymerase Chain Reaction:

    Article Title: Synergetic effect of yeast cell-surface expression of cellulase and expansin-like protein on direct ethanol production from cellulose
    Article Snippet: .. Total RNA was isolated from yeast cells cultivated in YPD medium for 48 h at 30°C using a RiboPure Yeast Kit (Ambion, Austin, TX, USA), and was then used for cDNA synthesis using a ReverTra Ace qPCR RT Kit (Toyobo). .. The synthesized cDNA was used as a template for real-time RT-PCR, which was performed with three sets of PCR primers: (BGL761(F) and BGL858(R), EGII968(F) and EGII1043(R), CBHII387(F) and CBHII455(R), and AoelpI567(F) and AoelpI637(R)) (Table ).

    Article Title: The Aryl Hydrocarbon Receptor Governs Epithelial Cell Invasion during Oropharyngeal Candidiasis
    Article Snippet: Paragraph title: RNA-seq and real-time PCR. ... Total epithelial cell RNA was isolated using the RiboPure yeast kit (Ambion), according to the manufacturer’s instructions.

    Article Title: The GRF10 homeobox gene regulates filamentous growth in the human fungal pathogen Candida albicans
    Article Snippet: Paragraph title: RNA preparation, cDNA synthesis and quantitative real-time PCR ... RNA was extracted from the frozen pellets using the RiboPure Yeast Kit (Ambion) following the manufacturer's instructions, except that less RNA (3 μg) was treated with DNaseI. cDNA was synthesized from 200 ng of RNA using the SensiFAST cDNA synthesis kit (Bioline), following the manufacturer's instructions.

    Microarray:

    Article Title: Gis1 and Rph1 Regulate Glycerol and Acetate Metabolism in Glucose Depleted Yeast Cells
    Article Snippet: .. Microarray experiments and data processing RNA was prepared from frozen yeast cells using the RiboPure™-Yeast kit (Ambion). .. The purified RNA was hybridized to Affymetrix YG-S98 arrays using the standard Affymetrix protocol.

    Article Title: Heterologous Expression Implicates a GATA Factor in Regulation of Nitrogen Metabolic Genes and Ion Homeostasis in the Halotolerant Yeast Debaryomyces hansenii †
    Article Snippet: Paragraph title: RNA preparation, DNA microarray analysis, and reverse transcription-PCR (RT-PCR). ... Yeast cells were harvested by sedimentation and washed with cold water, and total RNA was extracted using the RiboPure-Yeast kit (Ambion) according to the manufacturer's instructions.

    Random Hexamer Labeling:

    Article Title: Intra and Interspecific Variations of Gene Expression Levels in Yeast Are Largely Neutral: (Nei Lecture, SMBE 2016, Gold Coast)
    Article Snippet: Briefly, total RNA was extracted from each population using RiboPure-Yeast Kit (Ambion) and treated with DNase I to remove any contaminant DNA. .. First strand cDNA synthesis was performed using random hexamer priming (Superscript II, Invitrogen), followed by second strand cDNA synthesis (Invitrogen) as recommended by the manufacturer.

    Expressing:

    Article Title: A Multistate Toggle Switch Defines Fungal Cell Fates and Is Regulated by Synergistic Genetic Cues
    Article Snippet: Cells were removed from the medium and RNA isolated using the Ribopure-Yeast Kit (Ambion). .. RNA was treated with Turbo DNase (Ambion), and 500 ng of RNA used for cDNA generation using the GoScript enzyme (Promega). qRT-PCR was then performed by using primers in . cDNA levels were normalized to ACT1 expression levels.

    Article Title: The Histone Demethylase Activity of Rph1 is Not Essential for Its Role in the Transcriptional Response to Nutrient Signaling
    Article Snippet: RNA purification and reverse transcription PCR RNA was prepared from frozen yeast cells using the RiboPure-Yeast kit (Ambion). .. Expression of SSA3 was monitored with this cDNA as template and gene-specific primers (for primer sequences, see Orzechowski Westholm et al., 2012) using the competitive PCR strategy of Siebert and Larrick .

    Article Title: The GRF10 homeobox gene regulates filamentous growth in the human fungal pathogen Candida albicans
    Article Snippet: RNA was extracted from the frozen pellets using the RiboPure Yeast Kit (Ambion) following the manufacturer's instructions, except that less RNA (3 μg) was treated with DNaseI. cDNA was synthesized from 200 ng of RNA using the SensiFAST cDNA synthesis kit (Bioline), following the manufacturer's instructions. .. The relative gene expression was calculated by the ΔCT method using a reference gene as described by the Bio-Rad Laboratories qPCR manual.

    Transformation Assay:

    Article Title: Heterologous Expression Implicates a GATA Factor in Regulation of Nitrogen Metabolic Genes and Ion Homeostasis in the Halotolerant Yeast Debaryomyces hansenii †
    Article Snippet: Strain W303-1A was transformed with plasmid pYPGE15 or pDhGZF3 and grown in synthetic medium lacking uracil (containing 2% glucose as a carbon source and 38 mM ammonium sulfate as a nitrogen source) or in YPD medium up to an A 660 of 0.5. .. Yeast cells were harvested by sedimentation and washed with cold water, and total RNA was extracted using the RiboPure-Yeast kit (Ambion) according to the manufacturer's instructions.

    Sequencing:

    Article Title: Intra and Interspecific Variations of Gene Expression Levels in Yeast Are Largely Neutral: (Nei Lecture, SMBE 2016, Gold Coast)
    Article Snippet: Briefly, total RNA was extracted from each population using RiboPure-Yeast Kit (Ambion) and treated with DNase I to remove any contaminant DNA. .. End repair, A-tailing, and ligation of the Illumina adapters necessary for sequencing were then carried out using the NEBnext mRNA sample preparation kit (NEB).

    Article Title: The Aryl Hydrocarbon Receptor Governs Epithelial Cell Invasion during Oropharyngeal Candidiasis
    Article Snippet: Total epithelial cell RNA was isolated using the RiboPure yeast kit (Ambion), according to the manufacturer’s instructions. .. Using the HiSeq platform, 100 nucleotides of sequence was determined from each end of the cDNA fragments.

    Article Title: An Overview of Genes From Cyberlindnera americana, a Symbiont Yeast Isolated From the Gut of the Bark Beetle Dendroctonus rhizophagus (Curculionidae: Scolytinae), Involved in the Detoxification Process Using Genome and Transcriptome Data
    Article Snippet: Paragraph title: α-Pinene Treatment, RNA Extraction, and Sequencing ... Total RNA was extracted from each yeast culture with the RiboPure Yeast Kit (Ambion) according to the manufacturer’s protocol.

    Ligation:

    Article Title: Intra and Interspecific Variations of Gene Expression Levels in Yeast Are Largely Neutral: (Nei Lecture, SMBE 2016, Gold Coast)
    Article Snippet: Briefly, total RNA was extracted from each population using RiboPure-Yeast Kit (Ambion) and treated with DNase I to remove any contaminant DNA. .. End repair, A-tailing, and ligation of the Illumina adapters necessary for sequencing were then carried out using the NEBnext mRNA sample preparation kit (NEB).

    Infection:

    Article Title: The Aryl Hydrocarbon Receptor Governs Epithelial Cell Invasion during Oropharyngeal Candidiasis
    Article Snippet: For RNA-seq, OKF6/TERT-2 cells in six-well tissue culture plates were treated with either recombinant IFN-γ or medium alone for 24 h and then infected with 1 × 107 C. albicans yeast cells for 5 h in biological triplicates. .. Total epithelial cell RNA was isolated using the RiboPure yeast kit (Ambion), according to the manufacturer’s instructions.

    Light Microscopy:

    Article Title: The GRF10 homeobox gene regulates filamentous growth in the human fungal pathogen Candida albicans
    Article Snippet: To induce filamentation, the overnight culture was diluted 1:50, and cells were grown in 12-well dishes containing Spider medium with mild shaking at 37°C for 6 h; filamentation was confirmed by light microscopy. .. RNA was extracted from the frozen pellets using the RiboPure Yeast Kit (Ambion) following the manufacturer's instructions, except that less RNA (3 μg) was treated with DNaseI. cDNA was synthesized from 200 ng of RNA using the SensiFAST cDNA synthesis kit (Bioline), following the manufacturer's instructions.

    Sedimentation:

    Article Title: Heterologous Expression Implicates a GATA Factor in Regulation of Nitrogen Metabolic Genes and Ion Homeostasis in the Halotolerant Yeast Debaryomyces hansenii †
    Article Snippet: .. Yeast cells were harvested by sedimentation and washed with cold water, and total RNA was extracted using the RiboPure-Yeast kit (Ambion) according to the manufacturer's instructions. .. RNA quality was assessed by electrophoresis in a denaturing 0.8% agarose gel.

    Inhibition:

    Article Title: mRNA stability changes precede changes in steady-state mRNA amounts during hyperosmotic stress
    Article Snippet: No transcriptional inhibition was performed on samples collected 60 min post-NaCl shock. .. Cells were disrupted and RNA extracted using the RiboPure-yeast kit and instructions from the supplier (Applied Biosystems/Ambion).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Heterologous Expression Implicates a GATA Factor in Regulation of Nitrogen Metabolic Genes and Ion Homeostasis in the Halotolerant Yeast Debaryomyces hansenii †
    Article Snippet: Paragraph title: RNA preparation, DNA microarray analysis, and reverse transcription-PCR (RT-PCR). ... Yeast cells were harvested by sedimentation and washed with cold water, and total RNA was extracted using the RiboPure-Yeast kit (Ambion) according to the manufacturer's instructions.

    Article Title: A Multistate Toggle Switch Defines Fungal Cell Fates and Is Regulated by Synergistic Genetic Cues
    Article Snippet: Paragraph title: RT-PCR analysis ... Cells were removed from the medium and RNA isolated using the Ribopure-Yeast Kit (Ambion).

    Article Title: Synergetic effect of yeast cell-surface expression of cellulase and expansin-like protein on direct ethanol production from cellulose
    Article Snippet: Quantification of cellulase-encoding genes transcription by real-time RT-PCR The transcription level of each cellulase-encoding gene was quantified by real-time reverse transcription (RT)-PCR using a Stratagene MX3000P qPCR system and Thunderbird SYBR qPCR Mix (Toyobo, Osaka, Japan). .. Total RNA was isolated from yeast cells cultivated in YPD medium for 48 h at 30°C using a RiboPure Yeast Kit (Ambion, Austin, TX, USA), and was then used for cDNA synthesis using a ReverTra Ace qPCR RT Kit (Toyobo).

    Sonication:

    Article Title: Transcriptional responses of Candida glabrata biofilm cells to fluconazole are modulated by the carbon source
    Article Snippet: Then biofilms were scraped from the plates with 500 µL of PBS per well, sonicated for 30 s at 30% amplitude (Ultrasonic Processor, Cole-Parmer, IL, USA) in order to separate cells from matrix and then harvested by centrifugation at 8000 × g for 10 min at 4 °C. .. Cells drops were flash frozen in liquid nitrogen and total RNA was isolated from frozen cell pellets using the RiboPure Yeast kit (Ambion, Life Technologies, USA), according to manufacturer’s instructions.

    Recombinant:

    Article Title: The Aryl Hydrocarbon Receptor Governs Epithelial Cell Invasion during Oropharyngeal Candidiasis
    Article Snippet: For RNA-seq, OKF6/TERT-2 cells in six-well tissue culture plates were treated with either recombinant IFN-γ or medium alone for 24 h and then infected with 1 × 107 C. albicans yeast cells for 5 h in biological triplicates. .. Total epithelial cell RNA was isolated using the RiboPure yeast kit (Ambion), according to the manufacturer’s instructions.

    RNA Sequencing Assay:

    Article Title: Intra and Interspecific Variations of Gene Expression Levels in Yeast Are Largely Neutral: (Nei Lecture, SMBE 2016, Gold Coast)
    Article Snippet: Paragraph title: RNA Sequencing and Transcriptome Analysis ... Briefly, total RNA was extracted from each population using RiboPure-Yeast Kit (Ambion) and treated with DNase I to remove any contaminant DNA.

    Article Title: Comparison of the efficiency of different cell lysis methods and different commercial methods for RNA extraction from Candida albicans stored in RNAlater
    Article Snippet: Although several groups have extracted RNA from C. albicans , for example, to study differently expressed genes by RNA-seq [ , ], they did not report on the RNA yield. .. Hence, although all extraction methods succeeded to yield RNA after storage in TSB-G and RNAlater, the RiboPure Yeast Kit resulted in (mostly significantly) higher yields (as measured with NanoDrop, Fragment Analyzer, or RT-qPCR) compared to both other methods.

    Article Title: The Aryl Hydrocarbon Receptor Governs Epithelial Cell Invasion during Oropharyngeal Candidiasis
    Article Snippet: Paragraph title: RNA-seq and real-time PCR. ... Total epithelial cell RNA was isolated using the RiboPure yeast kit (Ambion), according to the manufacturer’s instructions.

    Isolation:

    Article Title: mRNA stability changes precede changes in steady-state mRNA amounts during hyperosmotic stress
    Article Snippet: Samples were divided in two; one of these was washed (twice) and RNA was isolated as below, in the other, transcription was stopped by the addition of the transcriptional inhibitor Phen (Sigma-Aldrich) to a final concentration of 100 ng/mL. .. Cells were disrupted and RNA extracted using the RiboPure-yeast kit and instructions from the supplier (Applied Biosystems/Ambion).

    Article Title: Transcriptional responses of Candida glabrata biofilm cells to fluconazole are modulated by the carbon source
    Article Snippet: .. Cells drops were flash frozen in liquid nitrogen and total RNA was isolated from frozen cell pellets using the RiboPure Yeast kit (Ambion, Life Technologies, USA), according to manufacturer’s instructions. .. All samples were treated with Turbo DNAse (Ambion) to remove residual DNA, according to manufacturer’s instructions.

    Article Title: A Multistate Toggle Switch Defines Fungal Cell Fates and Is Regulated by Synergistic Genetic Cues
    Article Snippet: .. Cells were removed from the medium and RNA isolated using the Ribopure-Yeast Kit (Ambion). .. RNA was treated with Turbo DNase (Ambion), and 500 ng of RNA used for cDNA generation using the GoScript enzyme (Promega). qRT-PCR was then performed by using primers in . cDNA levels were normalized to ACT1 expression levels.

    Article Title: Synergetic effect of yeast cell-surface expression of cellulase and expansin-like protein on direct ethanol production from cellulose
    Article Snippet: .. Total RNA was isolated from yeast cells cultivated in YPD medium for 48 h at 30°C using a RiboPure Yeast Kit (Ambion, Austin, TX, USA), and was then used for cDNA synthesis using a ReverTra Ace qPCR RT Kit (Toyobo). .. The synthesized cDNA was used as a template for real-time RT-PCR, which was performed with three sets of PCR primers: (BGL761(F) and BGL858(R), EGII968(F) and EGII1043(R), CBHII387(F) and CBHII455(R), and AoelpI567(F) and AoelpI637(R)) (Table ).

    Article Title: The Aryl Hydrocarbon Receptor Governs Epithelial Cell Invasion during Oropharyngeal Candidiasis
    Article Snippet: .. Total epithelial cell RNA was isolated using the RiboPure yeast kit (Ambion), according to the manufacturer’s instructions. .. The RNA was subjected to poly(A) enrichment by the TruSeq protocol, after which RNA-seq libraries (non-strand-specific, paired end) were prepared with the TruSeq RNA kit (Illumina).

    Purification:

    Article Title: Gis1 and Rph1 Regulate Glycerol and Acetate Metabolism in Glucose Depleted Yeast Cells
    Article Snippet: Microarray experiments and data processing RNA was prepared from frozen yeast cells using the RiboPure™-Yeast kit (Ambion). .. The purified RNA was hybridized to Affymetrix YG-S98 arrays using the standard Affymetrix protocol.

    Article Title: Heterologous Expression Implicates a GATA Factor in Regulation of Nitrogen Metabolic Genes and Ion Homeostasis in the Halotolerant Yeast Debaryomyces hansenii †
    Article Snippet: Yeast cells were harvested by sedimentation and washed with cold water, and total RNA was extracted using the RiboPure-Yeast kit (Ambion) according to the manufacturer's instructions. .. Fluorescent Cy3- and Cy5-labeled cDNAs were prepared from 8 μg of purified total RNA by the indirect dUTP-labeling method using the CyScribe Post-Labeling kit (Amersham Biosciences).

    Article Title: The Histone Demethylase Activity of Rph1 is Not Essential for Its Role in the Transcriptional Response to Nutrient Signaling
    Article Snippet: .. RNA purification and reverse transcription PCR RNA was prepared from frozen yeast cells using the RiboPure-Yeast kit (Ambion). .. For the reverse transcription PCR analysis , cDNA was produced using equal amounts of RNA from each sample as template in a reaction with oligo dT primers and RevertAid H Minus reverse transcriptase (Fermentas).

    Polymerase Chain Reaction:

    Article Title: Synergetic effect of yeast cell-surface expression of cellulase and expansin-like protein on direct ethanol production from cellulose
    Article Snippet: Total RNA was isolated from yeast cells cultivated in YPD medium for 48 h at 30°C using a RiboPure Yeast Kit (Ambion, Austin, TX, USA), and was then used for cDNA synthesis using a ReverTra Ace qPCR RT Kit (Toyobo). .. The synthesized cDNA was used as a template for real-time RT-PCR, which was performed with three sets of PCR primers: (BGL761(F) and BGL858(R), EGII968(F) and EGII1043(R), CBHII387(F) and CBHII455(R), and AoelpI567(F) and AoelpI637(R)) (Table ).

    Article Title: The Histone Demethylase Activity of Rph1 is Not Essential for Its Role in the Transcriptional Response to Nutrient Signaling
    Article Snippet: .. RNA purification and reverse transcription PCR RNA was prepared from frozen yeast cells using the RiboPure-Yeast kit (Ambion). .. For the reverse transcription PCR analysis , cDNA was produced using equal amounts of RNA from each sample as template in a reaction with oligo dT primers and RevertAid H Minus reverse transcriptase (Fermentas).

    Construct:

    Article Title: An Overview of Genes From Cyberlindnera americana, a Symbiont Yeast Isolated From the Gut of the Bark Beetle Dendroctonus rhizophagus (Curculionidae: Scolytinae), Involved in the Detoxification Process Using Genome and Transcriptome Data
    Article Snippet: Total RNA was extracted from each yeast culture with the RiboPure Yeast Kit (Ambion) according to the manufacturer’s protocol. .. Based on the manufacturer’s protocol, RNA libraries were constructed with the TruSeq Stranded mRNA Library Prep Kit (Illumina Inc., San Diego, CA, United States).

    Agarose Gel Electrophoresis:

    Article Title: Heterologous Expression Implicates a GATA Factor in Regulation of Nitrogen Metabolic Genes and Ion Homeostasis in the Halotolerant Yeast Debaryomyces hansenii †
    Article Snippet: Yeast cells were harvested by sedimentation and washed with cold water, and total RNA was extracted using the RiboPure-Yeast kit (Ambion) according to the manufacturer's instructions. .. RNA quality was assessed by electrophoresis in a denaturing 0.8% agarose gel.

    Plasmid Preparation:

    Article Title: Heterologous Expression Implicates a GATA Factor in Regulation of Nitrogen Metabolic Genes and Ion Homeostasis in the Halotolerant Yeast Debaryomyces hansenii †
    Article Snippet: Strain W303-1A was transformed with plasmid pYPGE15 or pDhGZF3 and grown in synthetic medium lacking uracil (containing 2% glucose as a carbon source and 38 mM ammonium sulfate as a nitrogen source) or in YPD medium up to an A 660 of 0.5. .. Yeast cells were harvested by sedimentation and washed with cold water, and total RNA was extracted using the RiboPure-Yeast kit (Ambion) according to the manufacturer's instructions.

    Electrophoresis:

    Article Title: Heterologous Expression Implicates a GATA Factor in Regulation of Nitrogen Metabolic Genes and Ion Homeostasis in the Halotolerant Yeast Debaryomyces hansenii †
    Article Snippet: Yeast cells were harvested by sedimentation and washed with cold water, and total RNA was extracted using the RiboPure-Yeast kit (Ambion) according to the manufacturer's instructions. .. RNA quality was assessed by electrophoresis in a denaturing 0.8% agarose gel.

    RNA Extraction:

    Article Title: An Overview of Genes From Cyberlindnera americana, a Symbiont Yeast Isolated From the Gut of the Bark Beetle Dendroctonus rhizophagus (Curculionidae: Scolytinae), Involved in the Detoxification Process Using Genome and Transcriptome Data
    Article Snippet: Paragraph title: α-Pinene Treatment, RNA Extraction, and Sequencing ... Total RNA was extracted from each yeast culture with the RiboPure Yeast Kit (Ambion) according to the manufacturer’s protocol.

    Sample Prep:

    Article Title: Intra and Interspecific Variations of Gene Expression Levels in Yeast Are Largely Neutral: (Nei Lecture, SMBE 2016, Gold Coast)
    Article Snippet: Briefly, total RNA was extracted from each population using RiboPure-Yeast Kit (Ambion) and treated with DNase I to remove any contaminant DNA. .. End repair, A-tailing, and ligation of the Illumina adapters necessary for sequencing were then carried out using the NEBnext mRNA sample preparation kit (NEB).

    Ethanol Precipitation:

    Article Title: Intra and Interspecific Variations of Gene Expression Levels in Yeast Are Largely Neutral: (Nei Lecture, SMBE 2016, Gold Coast)
    Article Snippet: Briefly, total RNA was extracted from each population using RiboPure-Yeast Kit (Ambion) and treated with DNase I to remove any contaminant DNA. .. Extraction of mRNA was carried out using MicroPoly(A)Purist Kit (Ambion) and 200 ng of the resulting mRNA sample was fragmented (Fragmentation Buffer, NEB) before ethanol precipitation.

    Incubation:

    Article Title: An Overview of Genes From Cyberlindnera americana, a Symbiont Yeast Isolated From the Gut of the Bark Beetle Dendroctonus rhizophagus (Curculionidae: Scolytinae), Involved in the Detoxification Process Using Genome and Transcriptome Data
    Article Snippet: A yeast culture without monoterpene (non-α-pinene stimulated, NPS) was included as control and incubated at the same conditions. .. Total RNA was extracted from each yeast culture with the RiboPure Yeast Kit (Ambion) according to the manufacturer’s protocol.

    Produced:

    Article Title: The Histone Demethylase Activity of Rph1 is Not Essential for Its Role in the Transcriptional Response to Nutrient Signaling
    Article Snippet: RNA purification and reverse transcription PCR RNA was prepared from frozen yeast cells using the RiboPure-Yeast kit (Ambion). .. For the reverse transcription PCR analysis , cDNA was produced using equal amounts of RNA from each sample as template in a reaction with oligo dT primers and RevertAid H Minus reverse transcriptase (Fermentas).

    Concentration Assay:

    Article Title: mRNA stability changes precede changes in steady-state mRNA amounts during hyperosmotic stress
    Article Snippet: Samples were divided in two; one of these was washed (twice) and RNA was isolated as below, in the other, transcription was stopped by the addition of the transcriptional inhibitor Phen (Sigma-Aldrich) to a final concentration of 100 ng/mL. .. Cells were disrupted and RNA extracted using the RiboPure-yeast kit and instructions from the supplier (Applied Biosystems/Ambion).

    Article Title: The Histone Demethylase Activity of Rph1 is Not Essential for Its Role in the Transcriptional Response to Nutrient Signaling
    Article Snippet: RNA purification and reverse transcription PCR RNA was prepared from frozen yeast cells using the RiboPure-Yeast kit (Ambion). .. Briefly, a short fragment of the SSA3 gene harboring a deletion of 116 bp was added as an internal control at a known concentration directly into the PCR reaction.

    Article Title: An Overview of Genes From Cyberlindnera americana, a Symbiont Yeast Isolated From the Gut of the Bark Beetle Dendroctonus rhizophagus (Curculionidae: Scolytinae), Involved in the Detoxification Process Using Genome and Transcriptome Data
    Article Snippet: The concentration of 0.1% (+)-α-pinene is higher than those found in the gut of some Dendroctonus species ( ; ). .. Total RNA was extracted from each yeast culture with the RiboPure Yeast Kit (Ambion) according to the manufacturer’s protocol.

    Lysis:

    Article Title: Comparison of the efficiency of different cell lysis methods and different commercial methods for RNA extraction from Candida albicans stored in RNAlater
    Article Snippet: Although we assessed that storage in RNAlater reduced cell lysis compared to storage in TSB-G, we observed that, when lysing cells with horizontal bead beating and subsequent extraction of RNA with the RiboPure Yeast Kit, storage in RNAlater did not decrease but increased RNA yield as compared to storage in TSB-G. To the contrary, RNA yield was reduced after storage in RNAlater when RNA was extracted with the RNeasy Mini Kit. .. Hence, although all extraction methods succeeded to yield RNA after storage in TSB-G and RNAlater, the RiboPure Yeast Kit resulted in (mostly significantly) higher yields (as measured with NanoDrop, Fragment Analyzer, or RT-qPCR) compared to both other methods.

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  • 99
    Thermo Fisher ribopure yeast rna purification kit
    Ribopure Yeast Rna Purification Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ribopure yeast rna purification kit/product/Thermo Fisher
    Average 99 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    ribopure yeast rna purification kit - by Bioz Stars, 2020-04
    99/100 stars
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    94
    Thermo Fisher ribopure yeast rna extraction kit
    Ribopure Yeast Rna Extraction Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ribopure yeast rna extraction kit/product/Thermo Fisher
    Average 94 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    ribopure yeast rna extraction kit - by Bioz Stars, 2020-04
    94/100 stars
      Buy from Supplier

    92
    Thermo Fisher yeast ribopure kit
    Yeast Ribopure Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/yeast ribopure kit/product/Thermo Fisher
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    yeast ribopure kit - by Bioz Stars, 2020-04
    92/100 stars
      Buy from Supplier

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