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PreAnalytiX ribonucleic acid rna
Ribonucleic Acid Rna, supplied by PreAnalytiX, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ribonucleic acid rna/product/PreAnalytiX
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
ribonucleic acid rna - by Bioz Stars, 2020-04
93/100 stars

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Modification:

Article Title: Whole Blood Gene Expression Reveals Specific Transcriptome Changes in Neonatal Encephalopathy
Article Snippet: One member of the research personnel performed a standardized neurological examination within 6 h after birth using the NICHD Neonatal Research Network (NRN) hypothermia trial encephalopathy staging (based on modified Sarnat staging). .. We collected 0.5 mL of peripheral venous or arterial blood in 1.4 mL ribonucleic acid (RNA)-stabilizing solution (PreAnalytiX, Qiagen/BD) within 12 h after birth, as per the manufacturer's instructions.

other:

Article Title: Whole-Blood Transcriptional Signatures Composed of Erythropoietic and NRF2-Regulated Genes Differ Between Cerebral Malaria and Severe Malarial Anemia
Article Snippet: Whole blood was collected for ribonucleic acid (RNA) using the PAXgene Blood RNA System (PreAnalytiX, Hombrechtikon, Switzerland).

Infection:

Article Title: Whole Blood Gene Expression Reveals Specific Transcriptome Changes in Neonatal Encephalopathy
Article Snippet: We collected 0.5 mL of peripheral venous or arterial blood in 1.4 mL ribonucleic acid (RNA)-stabilizing solution (PreAnalytiX, Qiagen/BD) within 12 h after birth, as per the manufacturer's instructions. .. In addition, all encephalopathic babies had detailed infection screens (blood culture, C-reactive protein, and a full blood count) and a structured neurological examination as a part of their standard clinical care.

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  • 94
    PreAnalytiX sanger sequencing rna
    Identification of two mutations in IFT27 . (A) Pedigree of the reported family with one affected individual and segregation analysis of the two IFT27 mutations. Example of Sanger sequencing profiles for the heterozygous individuals. (B) PCR amplification was performed on <t>RNA</t> extracted from blood of individual II.1 and a healthy unrelated control amplified between exon 2 and exon 7. (C) IFT27 <t>cDNA</t> scheme representing the obtained fragments with size and expected composition. PCR primers are positioned. (D) Sanger sequencing of normal F1 in a healthy unrelated control (left side showing each exon boundaries from exon 3 to 7) and cut and eluted F3 band in individual II.1 demonstrating the absence of exons 4 to 6 (right side).
    Sanger Sequencing Rna, supplied by PreAnalytiX, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sanger sequencing rna/product/PreAnalytiX
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sanger sequencing rna - by Bioz Stars, 2020-04
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    92
    PreAnalytiX rna
    Experimental design. Sixteen SPF pigs were treated (0 h) with Matrix M ( n = 8) or saline ( n = 8) the evening before a 2-h transport to the animal facility. At arrival (18 h), the SPF pigs were allotted to three rooms. SPF pigs given Matrix-M or saline were mixed in the first room (SPF Matrix-M/SPF and SPF Saline/SPF ; n = 4 + 4). Four hours later (22 h) eight conventionally reared pigs (Conv) arrived and were mixed with four of the SPF pigs given Matrix-M (SPF Matrix-M/Conv ) or four of those given saline (SPF Saline/Conv ; n = 4) in the other two rooms. Blood samples were collected from the SPF pigs in tubes without additives (serum), EDTA tubes and <t>PAXgene</t> Blood <t>RNA</t> tubes as indicated. Clinical examination was performed concurrently with blood sampling and at least once more each day. Post-mortem examination was performed on all pigs at termination of the experiment (6 days).
    Rna, supplied by PreAnalytiX, used in various techniques. Bioz Stars score: 92/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rna/product/PreAnalytiX
    Average 92 stars, based on 25 article reviews
    Price from $9.99 to $1999.99
    rna - by Bioz Stars, 2020-04
    92/100 stars
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    96
    PreAnalytiX paxgene tissue rna kit
    Schematic overview of the study setup. Human clinical or rat tissues were freshly collected, aliquoted and either directly snap-frozen (Cryo), or fixed in 4% neutral buffered formaldehyde (NBF) or in <t>PAXgene</t> Tissue Fix and Stabilizer. Paraffin embedded tissue blocks (PET) were stored under controlled temperatures for up to 7 years in case of human and up to 9 years in case of rat tissues until <t>RNA</t> and DNA extraction.
    Paxgene Tissue Rna Kit, supplied by PreAnalytiX, used in various techniques. Bioz Stars score: 96/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/paxgene tissue rna kit/product/PreAnalytiX
    Average 96 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    paxgene tissue rna kit - by Bioz Stars, 2020-04
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    99
    PreAnalytiX paxgene blood rna tubes
    Sample collection, processing and <t>RNA</t> extraction: Whole blood was collected in triplicate followed by peripheral blood mononuclear cells (PBMCs) separation in a subset of samples. The following sample collection tubes were used for the study: RNAgard ® , <t>PAXgene</t> ® RNA, EDTA, ACD-A, and CPT tubes. The PBMC separation was done using standard procedures for CPT tubes, and magnetic bead, LeukoLOCK ™ and LSM methods. The samples were then stored at either 4°C (Cold) or -80°C (Frozen) and shipped overnight (o/n) for follow-up RNA extraction. Next, they were treated with one or more of several different RNA extraction procedures: Biomaxi Precip Buffer/ PAXgene ® Blood miRNA, PAXgene ® Blood miRNA, TRIzol ® LS, ACK Lysing Buffer/ Qiagen miRNeasy, and TRIzol ® Reagent manufacturer’s protocol.
    Paxgene Blood Rna Tubes, supplied by PreAnalytiX, used in various techniques. Bioz Stars score: 99/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/paxgene blood rna tubes/product/PreAnalytiX
    Average 99 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
    paxgene blood rna tubes - by Bioz Stars, 2020-04
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    Identification of two mutations in IFT27 . (A) Pedigree of the reported family with one affected individual and segregation analysis of the two IFT27 mutations. Example of Sanger sequencing profiles for the heterozygous individuals. (B) PCR amplification was performed on RNA extracted from blood of individual II.1 and a healthy unrelated control amplified between exon 2 and exon 7. (C) IFT27 cDNA scheme representing the obtained fragments with size and expected composition. PCR primers are positioned. (D) Sanger sequencing of normal F1 in a healthy unrelated control (left side showing each exon boundaries from exon 3 to 7) and cut and eluted F3 band in individual II.1 demonstrating the absence of exons 4 to 6 (right side).

    Journal: Frontiers in Genetics

    Article Title: Identification and Characterization of Known Biallelic Mutations in the IFT27 (BBS19) Gene in a Novel Family With Bardet-Biedl Syndrome

    doi: 10.3389/fgene.2019.00021

    Figure Lengend Snippet: Identification of two mutations in IFT27 . (A) Pedigree of the reported family with one affected individual and segregation analysis of the two IFT27 mutations. Example of Sanger sequencing profiles for the heterozygous individuals. (B) PCR amplification was performed on RNA extracted from blood of individual II.1 and a healthy unrelated control amplified between exon 2 and exon 7. (C) IFT27 cDNA scheme representing the obtained fragments with size and expected composition. PCR primers are positioned. (D) Sanger sequencing of normal F1 in a healthy unrelated control (left side showing each exon boundaries from exon 3 to 7) and cut and eluted F3 band in individual II.1 demonstrating the absence of exons 4 to 6 (right side).

    Article Snippet: RNA Extraction, cDNA Synthesis, and Sanger Sequencing RNA was extracted from the patient’s blood using the PAXgene Blood RNA Kit (PreAnalytiX GmbH, Hombrechtikon, Switzerland).

    Techniques: Sequencing, Polymerase Chain Reaction, Amplification

    Experimental design. Sixteen SPF pigs were treated (0 h) with Matrix M ( n = 8) or saline ( n = 8) the evening before a 2-h transport to the animal facility. At arrival (18 h), the SPF pigs were allotted to three rooms. SPF pigs given Matrix-M or saline were mixed in the first room (SPF Matrix-M/SPF and SPF Saline/SPF ; n = 4 + 4). Four hours later (22 h) eight conventionally reared pigs (Conv) arrived and were mixed with four of the SPF pigs given Matrix-M (SPF Matrix-M/Conv ) or four of those given saline (SPF Saline/Conv ; n = 4) in the other two rooms. Blood samples were collected from the SPF pigs in tubes without additives (serum), EDTA tubes and PAXgene Blood RNA tubes as indicated. Clinical examination was performed concurrently with blood sampling and at least once more each day. Post-mortem examination was performed on all pigs at termination of the experiment (6 days).

    Journal: Veterinary Research

    Article Title: Innate immune responses induced by the saponin adjuvant Matrix-M in specific pathogen free pigs

    doi: 10.1186/s13567-017-0437-2

    Figure Lengend Snippet: Experimental design. Sixteen SPF pigs were treated (0 h) with Matrix M ( n = 8) or saline ( n = 8) the evening before a 2-h transport to the animal facility. At arrival (18 h), the SPF pigs were allotted to three rooms. SPF pigs given Matrix-M or saline were mixed in the first room (SPF Matrix-M/SPF and SPF Saline/SPF ; n = 4 + 4). Four hours later (22 h) eight conventionally reared pigs (Conv) arrived and were mixed with four of the SPF pigs given Matrix-M (SPF Matrix-M/Conv ) or four of those given saline (SPF Saline/Conv ; n = 4) in the other two rooms. Blood samples were collected from the SPF pigs in tubes without additives (serum), EDTA tubes and PAXgene Blood RNA tubes as indicated. Clinical examination was performed concurrently with blood sampling and at least once more each day. Post-mortem examination was performed on all pigs at termination of the experiment (6 days).

    Article Snippet: RNA from blood collected in PAXgene tubes during the in vivo experiment was extracted using the PAXgene Blood RNA Kit (PreAnalytiX) and samples with low RNA yield were concentrated with the E.Z.N.A.

    Techniques: Sampling

    Schematic overview of the study setup. Human clinical or rat tissues were freshly collected, aliquoted and either directly snap-frozen (Cryo), or fixed in 4% neutral buffered formaldehyde (NBF) or in PAXgene Tissue Fix and Stabilizer. Paraffin embedded tissue blocks (PET) were stored under controlled temperatures for up to 7 years in case of human and up to 9 years in case of rat tissues until RNA and DNA extraction.

    Journal: PLoS ONE

    Article Title: Impact of storage conditions on the quality of nucleic acids in paraffin embedded tissues

    doi: 10.1371/journal.pone.0203608

    Figure Lengend Snippet: Schematic overview of the study setup. Human clinical or rat tissues were freshly collected, aliquoted and either directly snap-frozen (Cryo), or fixed in 4% neutral buffered formaldehyde (NBF) or in PAXgene Tissue Fix and Stabilizer. Paraffin embedded tissue blocks (PET) were stored under controlled temperatures for up to 7 years in case of human and up to 9 years in case of rat tissues until RNA and DNA extraction.

    Article Snippet: RNA from human tissues was extracted from 5 μm sections (10–20 sections per tissue) using RNeasy FFPE Kit (QIAGEN GmbH, Hilden, Germany) for FFPE, the PAXgene Tissue RNA Kit (PreAnalytiX) for PFPE, or the Invitrogen TRIzol procedure (Thermo Fisher Scientific, Wilmington, DE) for 20–70 mg of snap-frozen tissues according to the manufacturer’s instructions.

    Techniques: Positron Emission Tomography, DNA Extraction

    Sample collection, processing and RNA extraction: Whole blood was collected in triplicate followed by peripheral blood mononuclear cells (PBMCs) separation in a subset of samples. The following sample collection tubes were used for the study: RNAgard ® , PAXgene ® RNA, EDTA, ACD-A, and CPT tubes. The PBMC separation was done using standard procedures for CPT tubes, and magnetic bead, LeukoLOCK ™ and LSM methods. The samples were then stored at either 4°C (Cold) or -80°C (Frozen) and shipped overnight (o/n) for follow-up RNA extraction. Next, they were treated with one or more of several different RNA extraction procedures: Biomaxi Precip Buffer/ PAXgene ® Blood miRNA, PAXgene ® Blood miRNA, TRIzol ® LS, ACK Lysing Buffer/ Qiagen miRNeasy, and TRIzol ® Reagent manufacturer’s protocol.

    Journal: PLoS ONE

    Article Title: Investigating gene expression profiles of whole blood and peripheral blood mononuclear cells using multiple collection and processing methods

    doi: 10.1371/journal.pone.0225137

    Figure Lengend Snippet: Sample collection, processing and RNA extraction: Whole blood was collected in triplicate followed by peripheral blood mononuclear cells (PBMCs) separation in a subset of samples. The following sample collection tubes were used for the study: RNAgard ® , PAXgene ® RNA, EDTA, ACD-A, and CPT tubes. The PBMC separation was done using standard procedures for CPT tubes, and magnetic bead, LeukoLOCK ™ and LSM methods. The samples were then stored at either 4°C (Cold) or -80°C (Frozen) and shipped overnight (o/n) for follow-up RNA extraction. Next, they were treated with one or more of several different RNA extraction procedures: Biomaxi Precip Buffer/ PAXgene ® Blood miRNA, PAXgene ® Blood miRNA, TRIzol ® LS, ACK Lysing Buffer/ Qiagen miRNeasy, and TRIzol ® Reagent manufacturer’s protocol.

    Article Snippet: PAXgene® blood RNA tubes: PAXgene® blood miRNA kit Whole blood collected in PAXgene® Blood RNA tubes was stored at -80°C for 1 to 2 days after being received, and then was thawed and incubated overnight at room temperature to ensure complete lysis of blood cells and maximize the mRNA yield.

    Techniques: RNA Extraction, Cycling Probe Technology

    Two dimensional Sammon projection using the top 50% of most variable transcripts (25370) for whole blood (red) and PBMC (black). There is a clear distinction in clusters for PBMCs and whole blood sample processing as marked by a black tilted line. All whole blood samples are shown on left side and PBMC samples are on the right side of the plot. The (F) and (C) denotes the frozen and cold conditions for the sample collection and storage. The description of all the legends is given here. PBMCs samples: ACD_Magnetic - blood collected in ACD-A tubes and separated using magnetic method followed by extraction using TRIzol ® method. CPT —blood collected in CPT tubes and PBMCs separation using manufacturer recommendations followed by extraction using TRIzol ® method. EDTA_LSM - blood collected in EDTA tube and PBMC separation using LSM followed by extraction using TRIzol ® method. EDTA_Leukolock - blood collected in EDTA tube, PBMCs separated using LeukoLOCK ™ method followed by extraction using TRIzol ® method. Whole blood samples: ACD - blood collected in ACD-A tube followed by RNA extraction using TRIzol ® LS method. ACD_RBC lysis- blood collected in ACD-A tube followed by RBC lysis using ACK buffer and RNA extraction using miRNAeasy extraction kit. EDTA_RBC lysis -blood collected in EDTA tube followed by RBC lysis using ACK buffer and RNA extraction using miRNAeasy extraction kit. EDTA - blood collected in EDTA tube followed by RNA extraction using TRIzol ® LS method. PAXgene - blood collected in PAXgene ® tube followed by RNA extraction using PAXgene ® blood miRNA kit. RNAgard— blood collected in RNAgard ® tube followed by RNA extraction using Biomaxi ™ Precip Buffer and PAXgene ® Blood miRNA kit.

    Journal: PLoS ONE

    Article Title: Investigating gene expression profiles of whole blood and peripheral blood mononuclear cells using multiple collection and processing methods

    doi: 10.1371/journal.pone.0225137

    Figure Lengend Snippet: Two dimensional Sammon projection using the top 50% of most variable transcripts (25370) for whole blood (red) and PBMC (black). There is a clear distinction in clusters for PBMCs and whole blood sample processing as marked by a black tilted line. All whole blood samples are shown on left side and PBMC samples are on the right side of the plot. The (F) and (C) denotes the frozen and cold conditions for the sample collection and storage. The description of all the legends is given here. PBMCs samples: ACD_Magnetic - blood collected in ACD-A tubes and separated using magnetic method followed by extraction using TRIzol ® method. CPT —blood collected in CPT tubes and PBMCs separation using manufacturer recommendations followed by extraction using TRIzol ® method. EDTA_LSM - blood collected in EDTA tube and PBMC separation using LSM followed by extraction using TRIzol ® method. EDTA_Leukolock - blood collected in EDTA tube, PBMCs separated using LeukoLOCK ™ method followed by extraction using TRIzol ® method. Whole blood samples: ACD - blood collected in ACD-A tube followed by RNA extraction using TRIzol ® LS method. ACD_RBC lysis- blood collected in ACD-A tube followed by RBC lysis using ACK buffer and RNA extraction using miRNAeasy extraction kit. EDTA_RBC lysis -blood collected in EDTA tube followed by RBC lysis using ACK buffer and RNA extraction using miRNAeasy extraction kit. EDTA - blood collected in EDTA tube followed by RNA extraction using TRIzol ® LS method. PAXgene - blood collected in PAXgene ® tube followed by RNA extraction using PAXgene ® blood miRNA kit. RNAgard— blood collected in RNAgard ® tube followed by RNA extraction using Biomaxi ™ Precip Buffer and PAXgene ® Blood miRNA kit.

    Article Snippet: PAXgene® blood RNA tubes: PAXgene® blood miRNA kit Whole blood collected in PAXgene® Blood RNA tubes was stored at -80°C for 1 to 2 days after being received, and then was thawed and incubated overnight at room temperature to ensure complete lysis of blood cells and maximize the mRNA yield.

    Techniques: Cycling Probe Technology, RNA Extraction, Lysis