ribonucleic acid from torula yeast  (Millipore)


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  • 94
    Name:
    Ribonucleic acid from torula yeast
    Description:

    Catalog Number:
    r6875
    Price:
    None
    Applications:
    Ribonucleic acid (RNA) from torula yeast may be used as a substrate for studying ribonuclease activities of enzymes such as ribonuclease-A, ribonuclease T1 (RNAase) and bougainvillea xbuttiana antiviral protein 1 (BBAP1).
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    Structured Review

    Millipore ribonucleic acid from torula yeast

    https://www.bioz.com/result/ribonucleic acid from torula yeast/product/Millipore
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ribonucleic acid from torula yeast - by Bioz Stars, 2020-07
    94/100 stars

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    Related Articles

    Isolation:

    Article Title: Purification and Characterization of Iso-Ribonucleases from a Novel Thermophilic Fungus
    Article Snippet: .. Preparation of Sample for RNase Purification Isolated cultures were transferred to Fernbach flasks containing 500 mL of YpSs broth containing 0.2% yeast RNA (Sigma Cat# R6625; Milwaukee, WI, USA). .. Each flask was placed on a rotary shaker set to 115 rpm and incubated at 55 °C.

    Purification:

    Article Title: Purification and Characterization of Iso-Ribonucleases from a Novel Thermophilic Fungus
    Article Snippet: .. Preparation of Sample for RNase Purification Isolated cultures were transferred to Fernbach flasks containing 500 mL of YpSs broth containing 0.2% yeast RNA (Sigma Cat# R6625; Milwaukee, WI, USA). .. Each flask was placed on a rotary shaker set to 115 rpm and incubated at 55 °C.

    Concentration Assay:

    Article Title: BMP and BMP receptor expression during murine organogenesis
    Article Snippet: .. Prehybridization solution (50% Formamide (Fisher BP227-100), 5xSSC, pH 4.5, 50μg/ml Ribonucleic acid from torula yeast, TypeVI (Sigma R6625), 1% SDS, 50μg/ml Heparin (Sigma H4784)) was added to probes to a concentration of 10μg/ml, as a stock solution and kept at −80°C. ..

    other:

    Article Title: In situ hybridization assay-based small molecule screening in zebrafish
    Article Snippet: 500 ml 100% formamide (Invitrogen, cat. no. 15515-026) 250 ml 20X SSC 1ml Tween-20 50 μg/ml heparin (Sigma, H3393) 500 μg/ml total yeast RNA (Sigma, R6625) Add DEPC H2 O to final volume of 1L Store up to 1 year at -20°C

    Activity Assay:

    Article Title: Purification of an Inducible DNase from a Thermophilic Fungus
    Article Snippet: .. The method used to detect RNase activity was identical to the described acid soluble assay method except yeast RNA (yeast RNA; 1mg/mL (Sigma R6625 Cat# R6625; Milwaukee, WI, USA) was substituted for the salmon sperm DNA. .. The membrane based system worked well and was faster than traditional column chromatography.

    Hybridization:

    Article Title: Mesencephalic basolateral domain specification is dependent on Sonic Hedgehog
    Article Snippet: .. The sections were washed in PBS-T and prehybridized for 1 h in hybridization buffer comprised of 50% deionized formamide (Amresco, 0606), SALT 1X (NaCl 0.2 M, Sigma S3014, tris-HCl 9 mM Sigma T3253, Tris-Base 1 mM, Sigma T6066, NaH2 PO4 ·2H2 O 5 mM, Scharlau SO0334, Na2 HPO4 5 mM, Sigma S3264 and EDTA 5 mM, Sigma E5134) Denharts 2X (Bio Basic Canada D0062), Dextran sulfate 0.2 mM (Amresco, 0198) and 0.1% tRNA (Sigma R6625). .. The RNA probes were obtained from Source Bioscience/ ImaGenes (Barhl1 , IRAVp968G10116D; Nhlh1 , IRAVp968D02101D) or constructions kindly provided by Dr. J. Rubenstein (Nkx2.2 ) and Dr. L. Puelles (Six3 ).

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  • 99
    Millipore yeast total rna
    Rai1 preferentially hydrolyzes unmethylated 5’ end capped <t>mRNA</t> in yeast cells Yeast strains harboring the temperature-sensitive ABD1 methyltransferase mutant allele, abd1 -5, or the abd1–5 rai1Δ double mutation were grown at the 37°C nonpermissive temperature for 45 min prior to transcriptional block with 5 µg/ml thiolutin. <t>RNA</t> was isolated from cells at the indicated time points following thiolutin addition and levels of RNA remaining determined by Northern blot analysis ( PGK1 and ACT1 ) or RT-qPCR ( CYH2 ). Half-lives (t 1/2 ) of the mRNAs were determined relative to the 18S rRNA and were derived from three independent experiments. The range of half-lives obtained are consistent with previously reported thiolutin-directed transcriptional arrest measurements 30 .
    Yeast Total Rna, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/yeast total rna/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    yeast total rna - by Bioz Stars, 2020-07
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    95
    Millipore yeast trna
    Rai1 preferentially hydrolyzes unmethylated 5’ end capped <t>mRNA</t> in yeast cells Yeast strains harboring the temperature-sensitive ABD1 methyltransferase mutant allele, abd1 -5, or the abd1–5 rai1Δ double mutation were grown at the 37°C nonpermissive temperature for 45 min prior to transcriptional block with 5 µg/ml thiolutin. <t>RNA</t> was isolated from cells at the indicated time points following thiolutin addition and levels of RNA remaining determined by Northern blot analysis ( PGK1 and ACT1 ) or RT-qPCR ( CYH2 ). Half-lives (t 1/2 ) of the mRNAs were determined relative to the 18S rRNA and were derived from three independent experiments. The range of half-lives obtained are consistent with previously reported thiolutin-directed transcriptional arrest measurements 30 .
    Yeast Trna, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 160 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/yeast trna/product/Millipore
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    97
    Millipore tripure isolation reagent
    Rai1 preferentially hydrolyzes unmethylated 5’ end capped <t>mRNA</t> in yeast cells Yeast strains harboring the temperature-sensitive ABD1 methyltransferase mutant allele, abd1 -5, or the abd1–5 rai1Δ double mutation were grown at the 37°C nonpermissive temperature for 45 min prior to transcriptional block with 5 µg/ml thiolutin. <t>RNA</t> was isolated from cells at the indicated time points following thiolutin addition and levels of RNA remaining determined by Northern blot analysis ( PGK1 and ACT1 ) or RT-qPCR ( CYH2 ). Half-lives (t 1/2 ) of the mRNAs were determined relative to the 18S rRNA and were derived from three independent experiments. The range of half-lives obtained are consistent with previously reported thiolutin-directed transcriptional arrest measurements 30 .
    Tripure Isolation Reagent, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tripure isolation reagent/product/Millipore
    Average 97 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    tripure isolation reagent - by Bioz Stars, 2020-07
    97/100 stars
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    Image Search Results


    Rai1 preferentially hydrolyzes unmethylated 5’ end capped mRNA in yeast cells Yeast strains harboring the temperature-sensitive ABD1 methyltransferase mutant allele, abd1 -5, or the abd1–5 rai1Δ double mutation were grown at the 37°C nonpermissive temperature for 45 min prior to transcriptional block with 5 µg/ml thiolutin. RNA was isolated from cells at the indicated time points following thiolutin addition and levels of RNA remaining determined by Northern blot analysis ( PGK1 and ACT1 ) or RT-qPCR ( CYH2 ). Half-lives (t 1/2 ) of the mRNAs were determined relative to the 18S rRNA and were derived from three independent experiments. The range of half-lives obtained are consistent with previously reported thiolutin-directed transcriptional arrest measurements 30 .

    Journal: Nature

    Article Title: Identification of a quality control mechanism for mRNA 5'-end capping

    doi: 10.1038/nature09338

    Figure Lengend Snippet: Rai1 preferentially hydrolyzes unmethylated 5’ end capped mRNA in yeast cells Yeast strains harboring the temperature-sensitive ABD1 methyltransferase mutant allele, abd1 -5, or the abd1–5 rai1Δ double mutation were grown at the 37°C nonpermissive temperature for 45 min prior to transcriptional block with 5 µg/ml thiolutin. RNA was isolated from cells at the indicated time points following thiolutin addition and levels of RNA remaining determined by Northern blot analysis ( PGK1 and ACT1 ) or RT-qPCR ( CYH2 ). Half-lives (t 1/2 ) of the mRNAs were determined relative to the 18S rRNA and were derived from three independent experiments. The range of half-lives obtained are consistent with previously reported thiolutin-directed transcriptional arrest measurements 30 .

    Article Snippet: Cap antibody immunoprecipitations and Northern blotting Methylated capped mRNA was immunoprecipitated (IP) from 20µg yeast total RNA with an agarose-conjugated 2,2,7 -trimethylguanosine antibody column (Calbiochem, San Diego, CA) which immunpurifies monomethyl capped mRNA as previously described – .

    Techniques: Mutagenesis, Blocking Assay, Isolation, Northern Blot, Quantitative RT-PCR, Derivative Assay

    Aberrantly capped mRNA levels increase in cells exposed to nutrient starvation ( a ) Amino acid starvation shifts mRNAs into the soluble mRNP fraction. The mid-log phase yeast strains were shifted to the indicated medium and grown for 45 min prior to fractionation. RNA was isolated from polysome-containing fractions sedimenting at 130,000 × g (P130) and the supernatant (S130) fraction, which contained the soluble mRNP. Distribution of the CYH2 mRNA from each fraction was determined by quantitative RT-PCR. The abd1–5 rai1 Δ double mutant strain grown at the permissive 25°C (methylated capped mRNA) or non-permissive 37°C (unmethylated capped mRNA) were used as a positive control. Results of three independent experiments are presented with error bars denoting +/− SD. ( b ) Aberrantly capped mRNAs are minimally affected by the Dcp2 decapping enzyme. The indicated strains were grown in complete medium at 22°C to an OD 600 of 0.6 and subsequently cultured in the same medium or amino acid minus medium for 45 min followed by the addition of thiolutin. The levels of CYH2 mRNA were determined by quantitative RT-PCR as in (a) above. ( c ) Methylated capped RNA was immunopurified utilizing monoclonal anti-trimethylguanosine antibody column from cells grown at the denoted culture conditions for 45 min and were detected by Northern Blot analysis. Quantitations for the mRNA cap methylation were normalized to total input RNA and 32 P-labeled methylated capped pcP RNA internal control (I.C.) and derived from three independent experiments. The error bars represent +/− SD.

    Journal: Nature

    Article Title: Identification of a quality control mechanism for mRNA 5'-end capping

    doi: 10.1038/nature09338

    Figure Lengend Snippet: Aberrantly capped mRNA levels increase in cells exposed to nutrient starvation ( a ) Amino acid starvation shifts mRNAs into the soluble mRNP fraction. The mid-log phase yeast strains were shifted to the indicated medium and grown for 45 min prior to fractionation. RNA was isolated from polysome-containing fractions sedimenting at 130,000 × g (P130) and the supernatant (S130) fraction, which contained the soluble mRNP. Distribution of the CYH2 mRNA from each fraction was determined by quantitative RT-PCR. The abd1–5 rai1 Δ double mutant strain grown at the permissive 25°C (methylated capped mRNA) or non-permissive 37°C (unmethylated capped mRNA) were used as a positive control. Results of three independent experiments are presented with error bars denoting +/− SD. ( b ) Aberrantly capped mRNAs are minimally affected by the Dcp2 decapping enzyme. The indicated strains were grown in complete medium at 22°C to an OD 600 of 0.6 and subsequently cultured in the same medium or amino acid minus medium for 45 min followed by the addition of thiolutin. The levels of CYH2 mRNA were determined by quantitative RT-PCR as in (a) above. ( c ) Methylated capped RNA was immunopurified utilizing monoclonal anti-trimethylguanosine antibody column from cells grown at the denoted culture conditions for 45 min and were detected by Northern Blot analysis. Quantitations for the mRNA cap methylation were normalized to total input RNA and 32 P-labeled methylated capped pcP RNA internal control (I.C.) and derived from three independent experiments. The error bars represent +/− SD.

    Article Snippet: Cap antibody immunoprecipitations and Northern blotting Methylated capped mRNA was immunoprecipitated (IP) from 20µg yeast total RNA with an agarose-conjugated 2,2,7 -trimethylguanosine antibody column (Calbiochem, San Diego, CA) which immunpurifies monomethyl capped mRNA as previously described – .

    Techniques: Fractionation, Isolation, Quantitative RT-PCR, Mutagenesis, Methylation, Positive Control, Cell Culture, Northern Blot, Labeling, Derivative Assay