ribonuclease inhibitor  (Thermo Fisher)


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    Name:
    Ribonuclease Inhibitor Cloned
    Description:
    Ribonuclease Inhibitor Cloned is a potent non competitive inhibitor of neutral pancreatic ribonucleases Applications Cell free translation systems 1 Reverse transcription of mRNA 2 Source Recombinant purified from E coli Performance and Quality Testing No detectable contaminating activity is observed in DNA nicking ribonuclease and protease assays Unit Definition One unit inhibits 5 ng RNase A by 50 using cytidine 2 3 cyclic monophosphate cCMP as the substrate 3
    Catalog Number:
    15518012
    Price:
    None
    Applications:
    PCR & Real-Time PCR|Reverse Transcription
    Category:
    Proteins Enzymes Peptides
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    Structured Review

    Thermo Fisher ribonuclease inhibitor
    Ribonuclease Inhibitor Cloned is a potent non competitive inhibitor of neutral pancreatic ribonucleases Applications Cell free translation systems 1 Reverse transcription of mRNA 2 Source Recombinant purified from E coli Performance and Quality Testing No detectable contaminating activity is observed in DNA nicking ribonuclease and protease assays Unit Definition One unit inhibits 5 ng RNase A by 50 using cytidine 2 3 cyclic monophosphate cCMP as the substrate 3
    https://www.bioz.com/result/ribonuclease inhibitor/product/Thermo Fisher
    Average 97 stars, based on 88 article reviews
    Price from $9.99 to $1999.99
    ribonuclease inhibitor - by Bioz Stars, 2020-08
    97/100 stars

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    Related Articles

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: FOXP3 gene expression in a tuberculosis case contact study
    Article Snippet: .. For reverse transcription–polymerase chain reaction (RT–PCR), total RNA was isolated from whole blood according to the manufacturer's instructions (PreAnalytiX; Qiagen Ltd) and reverse transcribed to cDNA using 1 µM oligo-dT15 (Sigma-Genosys, Cambridge, UK), 10 units ribonuclease inhibitor (Invitrogen Ltd, Paisley, UK) and following the instructions of the Omniscript RT Kit (Qiagen Ltd). .. Gene expression profiles of FOXP3 and IL-10 were measured by RT–PCR using the Corbett Research Rotorgene 3000 (Sydney, Australia) with QuantiTect SYBR Green PCR kits (Qiagen Ltd).

    Article Title: Complete genomic sequences, a key residue in the spike protein and deletions in non-structural protein 3b of US strains of the virulent and attenuated coronaviruses, transmissible gastroenteritis virus and porcine respiratory coronavirus
    Article Snippet: .. Reverse transcription and polymerase chain reactions (RT-PCR) were performed with 50-200 ng of coronavirus RNA supplemented with Ribonuclease inhibitor (RNASEOUT, Invitrogen, Carlsbad, California, USA ) using OneStep RT-PCR according to the manufacturers instructions (OneStep RT-PCR Kit, Qiagen, Valencia, CA, USA ). ..

    Isolation:

    Article Title: FOXP3 gene expression in a tuberculosis case contact study
    Article Snippet: .. For reverse transcription–polymerase chain reaction (RT–PCR), total RNA was isolated from whole blood according to the manufacturer's instructions (PreAnalytiX; Qiagen Ltd) and reverse transcribed to cDNA using 1 µM oligo-dT15 (Sigma-Genosys, Cambridge, UK), 10 units ribonuclease inhibitor (Invitrogen Ltd, Paisley, UK) and following the instructions of the Omniscript RT Kit (Qiagen Ltd). .. Gene expression profiles of FOXP3 and IL-10 were measured by RT–PCR using the Corbett Research Rotorgene 3000 (Sydney, Australia) with QuantiTect SYBR Green PCR kits (Qiagen Ltd).

    RNA Binding Assay:

    Article Title: Post‐translational modifications of Annexin A2 are linked to its association with perinuclear nonpolysomal mRNP complexes
    Article Snippet: .. The cytoskeletal fraction (~ 600 μg protein) of PC12 cells was diluted 1 : 4 in RNA‐binding buffer [10 mm Triethanolamine (pH 7.4), 50 mm NaCl, 1 mm DTT, 2 mm MgSO4 , 1 mm CaCl2 ] containing 1 mg·mL−1 yeast tRNA and 0.4 U·μL−1 ribonuclease inhibitor (Fermentas, ThermoFisher Scientific, Rockford, IL, USA). .. Subsequently, the fractions were incubated for 60 min with the anx A2 mRNA‐bound oligo(dT) magnetic beads at 4 °C on a platform shaker.

    Incubation:

    Article Title: Metallothionein-1 and nitric oxide expression are inversely correlated in a murine model of Chagas disease
    Article Snippet: .. The cDNA was synthesised using 4 μg of total RNA from liver tissue with 10 U of MMLV reverse transcriptase (Gibco BRL), 2 μL of 10 X buffer without Mg2+, 10 mM dNTPs stock solution [2.5 mM each of dA, dG, dC and dT (Strategene)], 50 mM MgCl2 (Gibco BRL), 10 mM oligo-d(T) (Gibco BRL), 5 U ribonuclease inhibitor (Gibco BRL) and 10 mM DTT (Gibco BRL) in 20 μL final volume, followed by incubation for 1 h at 37ºC. .. Primers were synthesised according to an alignment of the amino acid and nucleotide sequences reported for wild type MT-I mice , sequences MT-I (Fwd):TCCCGAGCATTACTAAA and MT-I (Rev): ACCCCAGTTTCTTATTG.

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  • 93
    Thermo Fisher rantes elisa kit
    miR-UL148D inhibits <t>RANTES</t> expression during viral infection. (A) Genomic location of UL150 and miR-UL148D (upper panel). The predicted mature sequence of miR-UL148D (blue) and its mutated residues at wobble position are shown (red) (bottom panel). (B) HFF cells were infected with Toledo-WT, ToledoΔmiR-UL148D or Toledo-Revertant. After extracting miRNAs from the infected cells, the detection of miR-UL148D was assessed using the RNase protection assay as described under “ Materials and Methods .” 5S rRNA was presented as a loading control stained by ethidium bromide. IE1 and gB gene expression was analyzed by RT-PCR. (C) Growth curves of Toledo-WT, Toledo-ΔmiR-UL148D and Toledo-Revertant. HFF cells were infected with wild-type, mutant and revertant viruses at an MOI of 2. The total number of cell-free viruses in the supernatants of infected cultures was determined by limiting dilution analyses. (D, E) After HFF cells were infected with Toledo-WT (white bars), ToledoΔmiR-UL148D (light gray bars) and Toledo-Revertant (dark gray bars), culture supernatants were harvested at the indicated post-infection time. The accumulated RANTES in supernatants was quantified by <t>ELISA</t> (D). RANTES mRNA level was detected by qRT-PCR (E). NI indicates non-infected control. Similar data were obtained in three independent experiments and the bars indicate mean ±S.D.
    Rantes Elisa Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rantes elisa kit/product/Thermo Fisher
    Average 93 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    rantes elisa kit - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

    93
    Thermo Fisher rac1
    Changes in the invasive phenotype of pancreatic cancer cells following GSK3β inhibition. (A) Phase-contrast microscopic findings (left panels), expression and subcellular localization of F-actin and Rac-1 (middle panels) and their merged images (right panels) in cancer cells along the wound edge (dashed line) were observed in the wound-healing assay in the presence of DMSO or AR-A014418 (AR). Arrows indicate lamellipodia. (B) Changes in the levels of active <t>(Rac1-GTP)</t> and total Rac1 examined by pull-down assay and Western blotting between the cancer cells treated with DMSO (DM) or 10 µM AR-A014418 (AR) for 24 hrs. (C) Changes in the secretion and mRNA expression of MMP-2 examined by gelatin zymography (left panel) and qRT-PCR (right panel) between PANC-1 cells treated with DMSO (DM) or AR-A014418 (AR) for 24 hrs. Values for the relative levels of mRNA expression are shown as means ± SDs of four separate experiments. * p
    Rac1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rac1/product/Thermo Fisher
    Average 93 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    rac1 - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

    Image Search Results


    miR-UL148D inhibits RANTES expression during viral infection. (A) Genomic location of UL150 and miR-UL148D (upper panel). The predicted mature sequence of miR-UL148D (blue) and its mutated residues at wobble position are shown (red) (bottom panel). (B) HFF cells were infected with Toledo-WT, ToledoΔmiR-UL148D or Toledo-Revertant. After extracting miRNAs from the infected cells, the detection of miR-UL148D was assessed using the RNase protection assay as described under “ Materials and Methods .” 5S rRNA was presented as a loading control stained by ethidium bromide. IE1 and gB gene expression was analyzed by RT-PCR. (C) Growth curves of Toledo-WT, Toledo-ΔmiR-UL148D and Toledo-Revertant. HFF cells were infected with wild-type, mutant and revertant viruses at an MOI of 2. The total number of cell-free viruses in the supernatants of infected cultures was determined by limiting dilution analyses. (D, E) After HFF cells were infected with Toledo-WT (white bars), ToledoΔmiR-UL148D (light gray bars) and Toledo-Revertant (dark gray bars), culture supernatants were harvested at the indicated post-infection time. The accumulated RANTES in supernatants was quantified by ELISA (D). RANTES mRNA level was detected by qRT-PCR (E). NI indicates non-infected control. Similar data were obtained in three independent experiments and the bars indicate mean ±S.D.

    Journal: PLoS Pathogens

    Article Title: Human Cytomegalovirus Clinical Strain-Specific microRNA miR-UL148D Targets the Human Chemokine RANTES during Infection

    doi: 10.1371/journal.ppat.1002577

    Figure Lengend Snippet: miR-UL148D inhibits RANTES expression during viral infection. (A) Genomic location of UL150 and miR-UL148D (upper panel). The predicted mature sequence of miR-UL148D (blue) and its mutated residues at wobble position are shown (red) (bottom panel). (B) HFF cells were infected with Toledo-WT, ToledoΔmiR-UL148D or Toledo-Revertant. After extracting miRNAs from the infected cells, the detection of miR-UL148D was assessed using the RNase protection assay as described under “ Materials and Methods .” 5S rRNA was presented as a loading control stained by ethidium bromide. IE1 and gB gene expression was analyzed by RT-PCR. (C) Growth curves of Toledo-WT, Toledo-ΔmiR-UL148D and Toledo-Revertant. HFF cells were infected with wild-type, mutant and revertant viruses at an MOI of 2. The total number of cell-free viruses in the supernatants of infected cultures was determined by limiting dilution analyses. (D, E) After HFF cells were infected with Toledo-WT (white bars), ToledoΔmiR-UL148D (light gray bars) and Toledo-Revertant (dark gray bars), culture supernatants were harvested at the indicated post-infection time. The accumulated RANTES in supernatants was quantified by ELISA (D). RANTES mRNA level was detected by qRT-PCR (E). NI indicates non-infected control. Similar data were obtained in three independent experiments and the bars indicate mean ±S.D.

    Article Snippet: A RANTES ELISA kit was purchased from Thermo Fisher Scientific Inc. (Rockford, IL).

    Techniques: Expressing, Infection, Sequencing, Rnase Protection Assay, Staining, Reverse Transcription Polymerase Chain Reaction, Mutagenesis, Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR

    PNA-based antisense oligonucleotides specific to miR-UL148D revert Toledo-induced inhibition of RANTES production. 2 days before HCMV infection, PNA-control or PNA-anti-miR-UL148D was transfected to HFF. After 48 h of infection, culture media and total RNA were analyzed by ELISA (A) and qRT-PCR (B). Down-regulation of miR-UL148D in the presence of PNA was detected by RNase protection assay (C). NI indicates non-infected control. Similar data were obtained in three independent experiments and the bars indicate mean ±S.D.

    Journal: PLoS Pathogens

    Article Title: Human Cytomegalovirus Clinical Strain-Specific microRNA miR-UL148D Targets the Human Chemokine RANTES during Infection

    doi: 10.1371/journal.ppat.1002577

    Figure Lengend Snippet: PNA-based antisense oligonucleotides specific to miR-UL148D revert Toledo-induced inhibition of RANTES production. 2 days before HCMV infection, PNA-control or PNA-anti-miR-UL148D was transfected to HFF. After 48 h of infection, culture media and total RNA were analyzed by ELISA (A) and qRT-PCR (B). Down-regulation of miR-UL148D in the presence of PNA was detected by RNase protection assay (C). NI indicates non-infected control. Similar data were obtained in three independent experiments and the bars indicate mean ±S.D.

    Article Snippet: A RANTES ELISA kit was purchased from Thermo Fisher Scientific Inc. (Rockford, IL).

    Techniques: Inhibition, Infection, Transfection, Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR, Rnase Protection Assay

    Changes in the invasive phenotype of pancreatic cancer cells following GSK3β inhibition. (A) Phase-contrast microscopic findings (left panels), expression and subcellular localization of F-actin and Rac-1 (middle panels) and their merged images (right panels) in cancer cells along the wound edge (dashed line) were observed in the wound-healing assay in the presence of DMSO or AR-A014418 (AR). Arrows indicate lamellipodia. (B) Changes in the levels of active (Rac1-GTP) and total Rac1 examined by pull-down assay and Western blotting between the cancer cells treated with DMSO (DM) or 10 µM AR-A014418 (AR) for 24 hrs. (C) Changes in the secretion and mRNA expression of MMP-2 examined by gelatin zymography (left panel) and qRT-PCR (right panel) between PANC-1 cells treated with DMSO (DM) or AR-A014418 (AR) for 24 hrs. Values for the relative levels of mRNA expression are shown as means ± SDs of four separate experiments. * p

    Journal: PLoS ONE

    Article Title: Aberrant Glycogen Synthase Kinase 3? Is Involved in Pancreatic Cancer Cell Invasion and Resistance to Therapy

    doi: 10.1371/journal.pone.0055289

    Figure Lengend Snippet: Changes in the invasive phenotype of pancreatic cancer cells following GSK3β inhibition. (A) Phase-contrast microscopic findings (left panels), expression and subcellular localization of F-actin and Rac-1 (middle panels) and their merged images (right panels) in cancer cells along the wound edge (dashed line) were observed in the wound-healing assay in the presence of DMSO or AR-A014418 (AR). Arrows indicate lamellipodia. (B) Changes in the levels of active (Rac1-GTP) and total Rac1 examined by pull-down assay and Western blotting between the cancer cells treated with DMSO (DM) or 10 µM AR-A014418 (AR) for 24 hrs. (C) Changes in the secretion and mRNA expression of MMP-2 examined by gelatin zymography (left panel) and qRT-PCR (right panel) between PANC-1 cells treated with DMSO (DM) or AR-A014418 (AR) for 24 hrs. Values for the relative levels of mRNA expression are shown as means ± SDs of four separate experiments. * p

    Article Snippet: Treatment with AR-A014418 decreased lamellipodia formation in cancer cells at the wound edge and resulted in diffuse cytoplasmic distribution of Rac1 and F-actin ( ).

    Techniques: Inhibition, Expressing, Wound Healing Assay, Pull Down Assay, Western Blot, Zymography, Quantitative RT-PCR