ribonuclease inhibitor  (Thermo Fisher)


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    Structured Review

    Thermo Fisher ribonuclease inhibitor
    Ribonuclease Inhibitor, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 79 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ribonuclease inhibitor/product/Thermo Fisher
    Average 94 stars, based on 79 article reviews
    Price from $9.99 to $1999.99
    ribonuclease inhibitor - by Bioz Stars, 2020-04
    94/100 stars

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    Related Articles

    SYBR Green Assay:

    Article Title: Integrated miRNA and mRNA expression profiling identifies novel targets and pathological mechanisms in autoimmune thyroid diseases
    Article Snippet: .. RNA from each sample was reverse transcribed for cDNA synthesis using high-capacity cDNA reverse transcription kit with a ribonuclease inhibitor (ThermoFisher), following the manufacturer's instructions. cDNAs were amplified using Power SYBR Green PCR master mix (ThermoFisher Scientific). .. Relative expression was determined using the log base 2 values of the difference Cts between mRNAs and the geometric mean of β-Actin and GAPDH (selected reference genes by NormFinder , Supplementary Table 1b).

    Amplification:

    Article Title: Integrated miRNA and mRNA expression profiling identifies novel targets and pathological mechanisms in autoimmune thyroid diseases
    Article Snippet: .. RNA from each sample was reverse transcribed for cDNA synthesis using high-capacity cDNA reverse transcription kit with a ribonuclease inhibitor (ThermoFisher), following the manufacturer's instructions. cDNAs were amplified using Power SYBR Green PCR master mix (ThermoFisher Scientific). .. Relative expression was determined using the log base 2 values of the difference Cts between mRNAs and the geometric mean of β-Actin and GAPDH (selected reference genes by NormFinder , Supplementary Table 1b).

    Article Title: Functional and Molecular Differences between Voltage-Gated K+ Channels of Fast-Spiking Interneurons and Pyramidal Neurons of Rat Hippocampus
    Article Snippet: The harvested material was then expelled under visual control into an autoclaved reaction tube containing hexamer random primers, deoxyribonucleoside triphosphates, dithiothreitol, ribonuclease inhibitor, and Superscript reverse transcriptase II (Life Technologies, Gaithersburg, MD), and incubated at 37°C for 1 hr. .. Subsequently, two rounds of PCR amplification with nested primer pairs were performed; the template for the second PCR was 1 μl of the first reaction.

    Polymerase Chain Reaction:

    Article Title: Integrated miRNA and mRNA expression profiling identifies novel targets and pathological mechanisms in autoimmune thyroid diseases
    Article Snippet: .. RNA from each sample was reverse transcribed for cDNA synthesis using high-capacity cDNA reverse transcription kit with a ribonuclease inhibitor (ThermoFisher), following the manufacturer's instructions. cDNAs were amplified using Power SYBR Green PCR master mix (ThermoFisher Scientific). .. Relative expression was determined using the log base 2 values of the difference Cts between mRNAs and the geometric mean of β-Actin and GAPDH (selected reference genes by NormFinder , Supplementary Table 1b).

    Article Title: Functional and Molecular Differences between Voltage-Gated K+ Channels of Fast-Spiking Interneurons and Pyramidal Neurons of Rat Hippocampus
    Article Snippet: The harvested material was then expelled under visual control into an autoclaved reaction tube containing hexamer random primers, deoxyribonucleoside triphosphates, dithiothreitol, ribonuclease inhibitor, and Superscript reverse transcriptase II (Life Technologies, Gaithersburg, MD), and incubated at 37°C for 1 hr. .. Subsequently, two rounds of PCR amplification with nested primer pairs were performed; the template for the second PCR was 1 μl of the first reaction.

    Next-Generation Sequencing:

    Article Title: Integrated miRNA and mRNA expression profiling identifies novel targets and pathological mechanisms in autoimmune thyroid diseases
    Article Snippet: Quantitative real-time RT-PCR for gene expression To validate the different expression levels of the mRNA genes determined by NGS, qPCR primers were selectively designed for 5 mRNA genes (STK36, INTU, ENO4, PACRG and KIF27 – Supplementary Table 1a for the sequence of primers) that were found to be differentially expressed in the discovery screen. β-Actin, GAPDH and HPRT were used as reference genes. .. RNA from each sample was reverse transcribed for cDNA synthesis using high-capacity cDNA reverse transcription kit with a ribonuclease inhibitor (ThermoFisher), following the manufacturer's instructions. cDNAs were amplified using Power SYBR Green PCR master mix (ThermoFisher Scientific).

    Transferring:

    Article Title: Functional and Molecular Differences between Voltage-Gated K+ Channels of Fast-Spiking Interneurons and Pyramidal Neurons of Rat Hippocampus
    Article Snippet: The cytoplasm of a whole-cell recorded neuron, either including (Kv2, Kv3, and Kv4) or excluding (Kv1) the nucleus, was harvested into the patch pipette by applying negative pressure. .. The harvested material was then expelled under visual control into an autoclaved reaction tube containing hexamer random primers, deoxyribonucleoside triphosphates, dithiothreitol, ribonuclease inhibitor, and Superscript reverse transcriptase II (Life Technologies, Gaithersburg, MD), and incubated at 37°C for 1 hr.

    Random Hexamer Labeling:

    Article Title: Molecular characterization of a new PToV strain. Evolutionary implications.
    Article Snippet: Briefly, 8 l of the RNA sample were mixed with a 1 mM dNTPs mix (Roche Applied Science) and 1 mM random hexamer primer (Roche Applied Science) and incubated for 10 min at 65 • C and 1 min on ice. .. Reverse transcription reaction was completed by adding 9 l of reaction mix containing 1x RT buffer, 10 mM DTT, 8 mM MgCl 2 , 40 U ribonuclease inhibitor (Fermentas) and 200 U of Superscript II reverse transcriptase and incubating for 10 min at 25 • C, followed by 45 min at 45 • C and finally 15 min at 65 • C. To Table 1 Oligonucleotide primers: location within torovirus genome and sequences.

    Quantitative RT-PCR:

    Article Title: Integrated miRNA and mRNA expression profiling identifies novel targets and pathological mechanisms in autoimmune thyroid diseases
    Article Snippet: Paragraph title: Quantitative real-time RT-PCR for gene expression ... RNA from each sample was reverse transcribed for cDNA synthesis using high-capacity cDNA reverse transcription kit with a ribonuclease inhibitor (ThermoFisher), following the manufacturer's instructions. cDNAs were amplified using Power SYBR Green PCR master mix (ThermoFisher Scientific).

    Real-time Polymerase Chain Reaction:

    Article Title: Integrated miRNA and mRNA expression profiling identifies novel targets and pathological mechanisms in autoimmune thyroid diseases
    Article Snippet: Quantitative real-time RT-PCR for gene expression To validate the different expression levels of the mRNA genes determined by NGS, qPCR primers were selectively designed for 5 mRNA genes (STK36, INTU, ENO4, PACRG and KIF27 – Supplementary Table 1a for the sequence of primers) that were found to be differentially expressed in the discovery screen. β-Actin, GAPDH and HPRT were used as reference genes. .. RNA from each sample was reverse transcribed for cDNA synthesis using high-capacity cDNA reverse transcription kit with a ribonuclease inhibitor (ThermoFisher), following the manufacturer's instructions. cDNAs were amplified using Power SYBR Green PCR master mix (ThermoFisher Scientific).

    Sequencing:

    Article Title: Integrated miRNA and mRNA expression profiling identifies novel targets and pathological mechanisms in autoimmune thyroid diseases
    Article Snippet: Quantitative real-time RT-PCR for gene expression To validate the different expression levels of the mRNA genes determined by NGS, qPCR primers were selectively designed for 5 mRNA genes (STK36, INTU, ENO4, PACRG and KIF27 – Supplementary Table 1a for the sequence of primers) that were found to be differentially expressed in the discovery screen. β-Actin, GAPDH and HPRT were used as reference genes. .. RNA from each sample was reverse transcribed for cDNA synthesis using high-capacity cDNA reverse transcription kit with a ribonuclease inhibitor (ThermoFisher), following the manufacturer's instructions. cDNAs were amplified using Power SYBR Green PCR master mix (ThermoFisher Scientific).

    Article Title: Functional and Molecular Differences between Voltage-Gated K+ Channels of Fast-Spiking Interneurons and Pyramidal Neurons of Rat Hippocampus
    Article Snippet: The harvested material was then expelled under visual control into an autoclaved reaction tube containing hexamer random primers, deoxyribonucleoside triphosphates, dithiothreitol, ribonuclease inhibitor, and Superscript reverse transcriptase II (Life Technologies, Gaithersburg, MD), and incubated at 37°C for 1 hr. .. The large sequence diversity of K+ channel subunits at the nucleotide level required the use of multiple sets of specific primers rather than a single set of degenerate primers.

    Incubation:

    Article Title: Molecular characterization of a new PToV strain. Evolutionary implications.
    Article Snippet: Briefly, 8 l of the RNA sample were mixed with a 1 mM dNTPs mix (Roche Applied Science) and 1 mM random hexamer primer (Roche Applied Science) and incubated for 10 min at 65 • C and 1 min on ice. .. Reverse transcription reaction was completed by adding 9 l of reaction mix containing 1x RT buffer, 10 mM DTT, 8 mM MgCl 2 , 40 U ribonuclease inhibitor (Fermentas) and 200 U of Superscript II reverse transcriptase and incubating for 10 min at 25 • C, followed by 45 min at 45 • C and finally 15 min at 65 • C. To Table 1 Oligonucleotide primers: location within torovirus genome and sequences.

    Article Title: Functional and Molecular Differences between Voltage-Gated K+ Channels of Fast-Spiking Interneurons and Pyramidal Neurons of Rat Hippocampus
    Article Snippet: .. The harvested material was then expelled under visual control into an autoclaved reaction tube containing hexamer random primers, deoxyribonucleoside triphosphates, dithiothreitol, ribonuclease inhibitor, and Superscript reverse transcriptase II (Life Technologies, Gaithersburg, MD), and incubated at 37°C for 1 hr. .. Subsequently, two rounds of PCR amplification with nested primer pairs were performed; the template for the second PCR was 1 μl of the first reaction.

    Expressing:

    Article Title: Integrated miRNA and mRNA expression profiling identifies novel targets and pathological mechanisms in autoimmune thyroid diseases
    Article Snippet: Paragraph title: Quantitative real-time RT-PCR for gene expression ... RNA from each sample was reverse transcribed for cDNA synthesis using high-capacity cDNA reverse transcription kit with a ribonuclease inhibitor (ThermoFisher), following the manufacturer's instructions. cDNAs were amplified using Power SYBR Green PCR master mix (ThermoFisher Scientific).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Functional and Molecular Differences between Voltage-Gated K+ Channels of Fast-Spiking Interneurons and Pyramidal Neurons of Rat Hippocampus
    Article Snippet: Patch pipettes used for RT-PCR experiments (0.8–3 MΩ) were filled with autoclaved internal solution containing (in m m ): 140 KCl, 5 EGTA, 3 MgCl2 , and 5 HEPES, pH-adjusted to 7.3 with KOH. .. The harvested material was then expelled under visual control into an autoclaved reaction tube containing hexamer random primers, deoxyribonucleoside triphosphates, dithiothreitol, ribonuclease inhibitor, and Superscript reverse transcriptase II (Life Technologies, Gaithersburg, MD), and incubated at 37°C for 1 hr.

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  • 96
    Thermo Fisher rac1
    Changes in the invasive phenotype of pancreatic cancer cells following GSK3β inhibition. (A) Phase-contrast microscopic findings (left panels), expression and subcellular localization of F-actin and Rac-1 (middle panels) and their merged images (right panels) in cancer cells along the wound edge (dashed line) were observed in the wound-healing assay in the presence of DMSO or AR-A014418 (AR). Arrows indicate lamellipodia. (B) Changes in the levels of active <t>(Rac1-GTP)</t> and total Rac1 examined by pull-down assay and Western blotting between the cancer cells treated with DMSO (DM) or 10 µM AR-A014418 (AR) for 24 hrs. (C) Changes in the secretion and mRNA expression of MMP-2 examined by gelatin zymography (left panel) and qRT-PCR (right panel) between PANC-1 cells treated with DMSO (DM) or AR-A014418 (AR) for 24 hrs. Values for the relative levels of mRNA expression are shown as means ± SDs of four separate experiments. * p
    Rac1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rac1/product/Thermo Fisher
    Average 96 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    rac1 - by Bioz Stars, 2020-04
    96/100 stars
      Buy from Supplier

    91
    Thermo Fisher mouse monoclonal anti rac1
    Activation of Rac by the processing of EphA4. (A) Effect of EphA4 ICD on reorganization of the actin cytoskeleton in NIH3T3 cells. Cells were transfected with HA or HA-EphA4 ICD, then stained with Alexa Fluor 546–phalloidin and the anti-HA antibody. EICD, EphA4 ICD. Bars, 20 µm. (B) Activation of <t>Rac1</t> by EphA4 ICD. NIH3T3 cells expressing HA-EphA4 ICD were subjected to a pull-down assay, followed by Western blotting using the anti-Rac1, the anti-Cdc42, and the anti-RhoA antibodies. The intensity of the activity was normalized to control. (C) Effects of various EphA4 mutants on the formation of lamellipodia. (C, left) Various EphA4 constructs. EphBD, Ephrin-binding domain; FN, fibronectin domain; TK, tyrosine kinase domain; SAM, SAM domain. (C, right) Quantification of the formation of lamellipodia-like structures. The efficiencies of lamellipodia formation induced by the indicated constructs were analyzed. (D) Inhibition of the EphA4 ICD-induced effect by Rac1 N17 . (D, left) Inhibition of EphA4 ICD-induced enhancement of the formation of lamellipodia by Rac1 N17 . NIH3T3 cells were cotransfected with HA-EphA4 ICD and Rac1 N17 , then analyzed. (D, right) Inhibition of EphA4 ICD-induced enhancement of the formation of dendritic spines by Rac1 N17 . Neurons were cotransfected with HA-EphA4 ICD and Rac1 N17 , then analyzed. The results represent three independent experiments. Data are expressed as means ± SEM; **, P
    Mouse Monoclonal Anti Rac1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse monoclonal anti rac1/product/Thermo Fisher
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse monoclonal anti rac1 - by Bioz Stars, 2020-04
    91/100 stars
      Buy from Supplier

    Image Search Results


    Changes in the invasive phenotype of pancreatic cancer cells following GSK3β inhibition. (A) Phase-contrast microscopic findings (left panels), expression and subcellular localization of F-actin and Rac-1 (middle panels) and their merged images (right panels) in cancer cells along the wound edge (dashed line) were observed in the wound-healing assay in the presence of DMSO or AR-A014418 (AR). Arrows indicate lamellipodia. (B) Changes in the levels of active (Rac1-GTP) and total Rac1 examined by pull-down assay and Western blotting between the cancer cells treated with DMSO (DM) or 10 µM AR-A014418 (AR) for 24 hrs. (C) Changes in the secretion and mRNA expression of MMP-2 examined by gelatin zymography (left panel) and qRT-PCR (right panel) between PANC-1 cells treated with DMSO (DM) or AR-A014418 (AR) for 24 hrs. Values for the relative levels of mRNA expression are shown as means ± SDs of four separate experiments. * p

    Journal: PLoS ONE

    Article Title: Aberrant Glycogen Synthase Kinase 3? Is Involved in Pancreatic Cancer Cell Invasion and Resistance to Therapy

    doi: 10.1371/journal.pone.0055289

    Figure Lengend Snippet: Changes in the invasive phenotype of pancreatic cancer cells following GSK3β inhibition. (A) Phase-contrast microscopic findings (left panels), expression and subcellular localization of F-actin and Rac-1 (middle panels) and their merged images (right panels) in cancer cells along the wound edge (dashed line) were observed in the wound-healing assay in the presence of DMSO or AR-A014418 (AR). Arrows indicate lamellipodia. (B) Changes in the levels of active (Rac1-GTP) and total Rac1 examined by pull-down assay and Western blotting between the cancer cells treated with DMSO (DM) or 10 µM AR-A014418 (AR) for 24 hrs. (C) Changes in the secretion and mRNA expression of MMP-2 examined by gelatin zymography (left panel) and qRT-PCR (right panel) between PANC-1 cells treated with DMSO (DM) or AR-A014418 (AR) for 24 hrs. Values for the relative levels of mRNA expression are shown as means ± SDs of four separate experiments. * p

    Article Snippet: Treatment with AR-A014418 decreased lamellipodia formation in cancer cells at the wound edge and resulted in diffuse cytoplasmic distribution of Rac1 and F-actin ( ).

    Techniques: Inhibition, Expressing, Wound Healing Assay, Pull Down Assay, Western Blot, Zymography, Quantitative RT-PCR

    Activation of Rac by the processing of EphA4. (A) Effect of EphA4 ICD on reorganization of the actin cytoskeleton in NIH3T3 cells. Cells were transfected with HA or HA-EphA4 ICD, then stained with Alexa Fluor 546–phalloidin and the anti-HA antibody. EICD, EphA4 ICD. Bars, 20 µm. (B) Activation of Rac1 by EphA4 ICD. NIH3T3 cells expressing HA-EphA4 ICD were subjected to a pull-down assay, followed by Western blotting using the anti-Rac1, the anti-Cdc42, and the anti-RhoA antibodies. The intensity of the activity was normalized to control. (C) Effects of various EphA4 mutants on the formation of lamellipodia. (C, left) Various EphA4 constructs. EphBD, Ephrin-binding domain; FN, fibronectin domain; TK, tyrosine kinase domain; SAM, SAM domain. (C, right) Quantification of the formation of lamellipodia-like structures. The efficiencies of lamellipodia formation induced by the indicated constructs were analyzed. (D) Inhibition of the EphA4 ICD-induced effect by Rac1 N17 . (D, left) Inhibition of EphA4 ICD-induced enhancement of the formation of lamellipodia by Rac1 N17 . NIH3T3 cells were cotransfected with HA-EphA4 ICD and Rac1 N17 , then analyzed. (D, right) Inhibition of EphA4 ICD-induced enhancement of the formation of dendritic spines by Rac1 N17 . Neurons were cotransfected with HA-EphA4 ICD and Rac1 N17 , then analyzed. The results represent three independent experiments. Data are expressed as means ± SEM; **, P

    Journal: The Journal of Cell Biology

    Article Title: Synaptic activity prompts ?-secretase-mediated cleavage of EphA4 and dendritic spine formation

    doi: 10.1083/jcb.200809151

    Figure Lengend Snippet: Activation of Rac by the processing of EphA4. (A) Effect of EphA4 ICD on reorganization of the actin cytoskeleton in NIH3T3 cells. Cells were transfected with HA or HA-EphA4 ICD, then stained with Alexa Fluor 546–phalloidin and the anti-HA antibody. EICD, EphA4 ICD. Bars, 20 µm. (B) Activation of Rac1 by EphA4 ICD. NIH3T3 cells expressing HA-EphA4 ICD were subjected to a pull-down assay, followed by Western blotting using the anti-Rac1, the anti-Cdc42, and the anti-RhoA antibodies. The intensity of the activity was normalized to control. (C) Effects of various EphA4 mutants on the formation of lamellipodia. (C, left) Various EphA4 constructs. EphBD, Ephrin-binding domain; FN, fibronectin domain; TK, tyrosine kinase domain; SAM, SAM domain. (C, right) Quantification of the formation of lamellipodia-like structures. The efficiencies of lamellipodia formation induced by the indicated constructs were analyzed. (D) Inhibition of the EphA4 ICD-induced effect by Rac1 N17 . (D, left) Inhibition of EphA4 ICD-induced enhancement of the formation of lamellipodia by Rac1 N17 . NIH3T3 cells were cotransfected with HA-EphA4 ICD and Rac1 N17 , then analyzed. (D, right) Inhibition of EphA4 ICD-induced enhancement of the formation of dendritic spines by Rac1 N17 . Neurons were cotransfected with HA-EphA4 ICD and Rac1 N17 , then analyzed. The results represent three independent experiments. Data are expressed as means ± SEM; **, P

    Article Snippet: The following antibodies were used: rabbit polyclonal anti-EphA4 (Millipore), rabbit polyclonal anti-GluR1 (Millipore), rabbit polyclonal anti–phospho-GluR1 Ser845 (Millipore), rabbit polyclonal anti-Nicastrin (Sigma-Aldrich), rabbit polyclonal anti-PS1 (Sigma-Aldrich), rabbit polyclonal anti-PS2 (EMD), mouse monoclonal anti-Bassoon (Assay Designs), mouse monoclonal anti-PSD-95 (Thermo Fisher Scientific), mouse monoclonal anti-synaptophysin (Millipore), mouse monoclonal anti–Flotillin-1 (BD), mouse monoclonal anti-NMDA receptor 1 (BD), mouse monoclonal anti-Rac1 (Thermo Fisher Scientific), mouse monoclonal anti-Cdc42 (BD), mouse monoclonal anti-RhoA (Santa Cruz Biotechnology, Inc.), mouse monoclonal phospho-tyrosine (pY20; Santa Cruz Biotechnology, Inc.), rat polyclonal anti-Homer (Abcam), rabbit polyclonal anti-GFP (Invitrogen), and rat monoclonal anti-HA (3F10; Roche).

    Techniques: Activation Assay, Transfection, Staining, Expressing, Pull Down Assay, Western Blot, Activity Assay, Construct, Binding Assay, Inhibition