ribominus eukaryote system v2  (Thermo Fisher)


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    Structured Review

    Thermo Fisher ribominus eukaryote system v2
    Ribominus Eukaryote System V2, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ribominus eukaryote system v2/product/Thermo Fisher
    Average 96 stars, based on 20 article reviews
    Price from $9.99 to $1999.99
    ribominus eukaryote system v2 - by Bioz Stars, 2020-01
    96/100 stars

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    Related Articles

    Selection:

    Article Title: High-Resolution Mapping of N6-Methyladenosine Using m6A Crosslinking Immunoprecipitation Sequencing (m6A-CLIP-Seq)
    Article Snippet: .. However, if the sequencing results show that there is too much ribosomal RNA, it is possible to perform a second round of poly (A) selection using the GenElute kit, or to further deplete ribosomal RNA using RiboMinus Eukaryote System v2 (Ambion). ..

    RNA Sequencing Assay:

    Article Title: Dysregulation of MicroRNA Regulatory Network in Lower Extremities Arterial Disease
    Article Snippet: In order to increase the percentage of coding mRNA, total RNA samples were subjected to ribodepletion procedure using RiboMinus Eukaryote System v2 (Ambion, USA), according to manufacturer’s protocol. .. Efficiency of rRNA depletion process was verified using Agilent 2100 Bioanalyzer with Agilent RNA 6000 Pico Kit. rRNA-depleted RNA samples were subsequently subjected to transcriptome libraries preparation procedure using the components supplied with Ion Total RNA-Seq Kit v2, Ion Xpress RNA-Seq Barcode 01-16 Kit, and Magnetic Bead Cleanup Module Kit.

    Article Title: The mitochondrial transcriptome of the anglerfish Lophius piscatorius
    Article Snippet: .. Cellular rRNA was depleted from 1 μg of total RNA using the RiboMinus™ Eukaryote System v2 (Thermo Fisher Scientific, Waltham, MA—USA), and whole transcriptome library was constructed using the Ion Total RNA-seq kit v2 (Thermo Fisher Scientific) according to the manufacturers protocols. .. Manual template preparation on an Ion OneTouch™ 2 System (Thermo Fisher Scientific) and sequencing of two Ion 540™ chips on the Ion GeneStudio™ S5 System (Thermo Fisher Scientific) were carried out at our Genomics Platform (Nord University) according to the manufacturers protocols.

    Isolation:

    Article Title: Dysregulation of MicroRNA Regulatory Network in Lower Extremities Arterial Disease
    Article Snippet: Transcriptome Sequencing Due to technical limitations, transcriptome libraries were prepared from 15 total RNA samples isolated from randomly selected representative PBMCs samples (eight from LEAD patients and seven from controls). .. In order to increase the percentage of coding mRNA, total RNA samples were subjected to ribodepletion procedure using RiboMinus Eukaryote System v2 (Ambion, USA), according to manufacturer’s protocol.

    Article Title: The mitochondrial transcriptome of the anglerfish Lophius piscatorius
    Article Snippet: Total RNA from heart muscle tissue of specimen BF2 was isolated using QIAzol Lysis Reagent (QIAGEN, Hilden—Germany) according to the manufacturers protocol. .. Cellular rRNA was depleted from 1 μg of total RNA using the RiboMinus™ Eukaryote System v2 (Thermo Fisher Scientific, Waltham, MA—USA), and whole transcriptome library was constructed using the Ion Total RNA-seq kit v2 (Thermo Fisher Scientific) according to the manufacturers protocols.

    Transferring:

    Article Title: High-Resolution Mapping of N6-Methyladenosine Using m6A Crosslinking Immunoprecipitation Sequencing (m6A-CLIP-Seq)
    Article Snippet: If using easily detached cells, such as HEK293T, pipette the ice-cold PBS gently onto the edge of the cell culture dish when washing to minimize loss of cells. .. However, if the sequencing results show that there is too much ribosomal RNA, it is possible to perform a second round of poly (A) selection using the GenElute kit, or to further deplete ribosomal RNA using RiboMinus Eukaryote System v2 (Ambion).

    Northern Blot:

    Article Title: The mitochondrial transcriptome of the anglerfish Lophius piscatorius
    Article Snippet: Nucleic acid extraction and high-throughput sequencing Lophius piscatorius tissue samples were collected from two specimens obtained by commercial fishery off the coast of Nordland County, Northern Norway, in 2015 (BF1) and 2017 (BF2). .. Cellular rRNA was depleted from 1 μg of total RNA using the RiboMinus™ Eukaryote System v2 (Thermo Fisher Scientific, Waltham, MA—USA), and whole transcriptome library was constructed using the Ion Total RNA-seq kit v2 (Thermo Fisher Scientific) according to the manufacturers protocols.

    Construct:

    Article Title: The mitochondrial transcriptome of the anglerfish Lophius piscatorius
    Article Snippet: .. Cellular rRNA was depleted from 1 μg of total RNA using the RiboMinus™ Eukaryote System v2 (Thermo Fisher Scientific, Waltham, MA—USA), and whole transcriptome library was constructed using the Ion Total RNA-seq kit v2 (Thermo Fisher Scientific) according to the manufacturers protocols. .. Manual template preparation on an Ion OneTouch™ 2 System (Thermo Fisher Scientific) and sequencing of two Ion 540™ chips on the Ion GeneStudio™ S5 System (Thermo Fisher Scientific) were carried out at our Genomics Platform (Nord University) according to the manufacturers protocols.

    Article Title: High-Resolution Mapping of N6-Methyladenosine Using m6A Crosslinking Immunoprecipitation Sequencing (m6A-CLIP-Seq)
    Article Snippet: However, if the sequencing results show that there is too much ribosomal RNA, it is possible to perform a second round of poly (A) selection using the GenElute kit, or to further deplete ribosomal RNA using RiboMinus Eukaryote System v2 (Ambion). .. Be sure to use at least 1 μg of mRNA as starting material, or it may not be possible to construct the library.

    Purification:

    Article Title: High-Resolution Mapping of N6-Methyladenosine Using m6A Crosslinking Immunoprecipitation Sequencing (m6A-CLIP-Seq)
    Article Snippet: It is generally not necessary to remove any trace amounts of ribosomal RNA left over from the mRNA purification step, as the majority of the reads will be of mRNA. .. However, if the sequencing results show that there is too much ribosomal RNA, it is possible to perform a second round of poly (A) selection using the GenElute kit, or to further deplete ribosomal RNA using RiboMinus Eukaryote System v2 (Ambion).

    Sequencing:

    Article Title: Dysregulation of MicroRNA Regulatory Network in Lower Extremities Arterial Disease
    Article Snippet: Paragraph title: Transcriptome Sequencing ... In order to increase the percentage of coding mRNA, total RNA samples were subjected to ribodepletion procedure using RiboMinus Eukaryote System v2 (Ambion, USA), according to manufacturer’s protocol.

    Article Title: The mitochondrial transcriptome of the anglerfish Lophius piscatorius
    Article Snippet: Paragraph title: Nucleic acid extraction and high-throughput sequencing ... Cellular rRNA was depleted from 1 μg of total RNA using the RiboMinus™ Eukaryote System v2 (Thermo Fisher Scientific, Waltham, MA—USA), and whole transcriptome library was constructed using the Ion Total RNA-seq kit v2 (Thermo Fisher Scientific) according to the manufacturers protocols.

    Article Title: High-Resolution Mapping of N6-Methyladenosine Using m6A Crosslinking Immunoprecipitation Sequencing (m6A-CLIP-Seq)
    Article Snippet: .. However, if the sequencing results show that there is too much ribosomal RNA, it is possible to perform a second round of poly (A) selection using the GenElute kit, or to further deplete ribosomal RNA using RiboMinus Eukaryote System v2 (Ambion). ..

    Cell Culture:

    Article Title: High-Resolution Mapping of N6-Methyladenosine Using m6A Crosslinking Immunoprecipitation Sequencing (m6A-CLIP-Seq)
    Article Snippet: If using easily detached cells, such as HEK293T, pipette the ice-cold PBS gently onto the edge of the cell culture dish when washing to minimize loss of cells. .. However, if the sequencing results show that there is too much ribosomal RNA, it is possible to perform a second round of poly (A) selection using the GenElute kit, or to further deplete ribosomal RNA using RiboMinus Eukaryote System v2 (Ambion).

    High Throughput Screening Assay:

    Article Title: The mitochondrial transcriptome of the anglerfish Lophius piscatorius
    Article Snippet: Paragraph title: Nucleic acid extraction and high-throughput sequencing ... Cellular rRNA was depleted from 1 μg of total RNA using the RiboMinus™ Eukaryote System v2 (Thermo Fisher Scientific, Waltham, MA—USA), and whole transcriptome library was constructed using the Ion Total RNA-seq kit v2 (Thermo Fisher Scientific) according to the manufacturers protocols.

    Lysis:

    Article Title: The mitochondrial transcriptome of the anglerfish Lophius piscatorius
    Article Snippet: Total RNA from heart muscle tissue of specimen BF2 was isolated using QIAzol Lysis Reagent (QIAGEN, Hilden—Germany) according to the manufacturers protocol. .. Cellular rRNA was depleted from 1 μg of total RNA using the RiboMinus™ Eukaryote System v2 (Thermo Fisher Scientific, Waltham, MA—USA), and whole transcriptome library was constructed using the Ion Total RNA-seq kit v2 (Thermo Fisher Scientific) according to the manufacturers protocols.

    DNA Sequencing:

    Article Title: The mitochondrial transcriptome of the anglerfish Lophius piscatorius
    Article Snippet: Total DNA sequencing (head kidney) of BF2 using the Illumina HiSeqX platform was performed by Dovetail Genomics (Chicago, US) as a service [ ]. .. Cellular rRNA was depleted from 1 μg of total RNA using the RiboMinus™ Eukaryote System v2 (Thermo Fisher Scientific, Waltham, MA—USA), and whole transcriptome library was constructed using the Ion Total RNA-seq kit v2 (Thermo Fisher Scientific) according to the manufacturers protocols.

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  • 90
    Thermo Fisher ribominus eukaryote kit v2
    Ribominus Eukaryote Kit V2, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ribominus eukaryote kit v2/product/Thermo Fisher
    Average 90 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    ribominus eukaryote kit v2 - by Bioz Stars, 2020-01
    90/100 stars
      Buy from Supplier

    90
    Thermo Fisher low input ribominus eukaryote system v2
    Low Input Ribominus Eukaryote System V2, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/low input ribominus eukaryote system v2/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    low input ribominus eukaryote system v2 - by Bioz Stars, 2020-01
    90/100 stars
      Buy from Supplier

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