ribominus eukaryote system v2  (Thermo Fisher)


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    Name:
    RiboMinus Eukaryote System v2
    Description:
    The RiboMinus Eukaryote System v2 provides truly complete transcriptome isolation for next generation sequencing analysis by offering selective depletion of up to 99 9 of the cytoplasmic 5S 5 8S 18S and 28S and mitochondrial 12S and 16S ribosomal RNA from total RNA This selection system contrasts with poly A and cap binding methods which introduce bias and select for only part of the transcriptome Features of the RiboMinus Eukaryote System v2 include • Magnetic bead based purification for rapid seamless purification• A 1 hour workflow• An input range of 1 5 µgThe RiboMinus Eukaryote System v2 improves upon the previous system RiboMinus Eukaryote Kit for RNA Seq by removing significantly more rRNA both cytoplasmic and mitochondrial from the total RNA When used with our library construction kits for sequencing the RiboMinus Eukaryote System v2 provides maximum mapping to reference database with minimum bias maintaining relative gene expression levels of even smaller RNA species The resulting total RNA after depletion contains minimum contaminating rRNA with reduced variability The RiboMinus Eukaryote System v2 is designed for the removal of human mouse and rat rRNA A magnetic bead based purification module is included for purification of the whole transcriptome without altering the expression levels of small or non coding RNA For column based purification please see the RiboMinus Eukaryote Kit v2 The RiboMinus Eukaryote System v2 has an RNA input range of 1 5 µg For lower inputs down to 100 ng the Low Input RiboMinus Eukaryote System v2 is recommended The RiboMinus Eukaryote System v2 contains sufficient reagents to perform 12 ribosomal RNA depletions
    Catalog Number:
    a15026
    Price:
    None
    Applications:
    DNA & RNA Purification & Analysis|Epigenetic Sequencing|RNA Sequencing|RNA Extraction|Transcriptome RNA Isolation|Small RNA & miRNA Sequencing|Whole Transcriptome Sequencing|Sequencing
    Category:
    Kits and Assays
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    Structured Review

    Thermo Fisher ribominus eukaryote system v2
    The RiboMinus Eukaryote System v2 provides truly complete transcriptome isolation for next generation sequencing analysis by offering selective depletion of up to 99 9 of the cytoplasmic 5S 5 8S 18S and 28S and mitochondrial 12S and 16S ribosomal RNA from total RNA This selection system contrasts with poly A and cap binding methods which introduce bias and select for only part of the transcriptome Features of the RiboMinus Eukaryote System v2 include • Magnetic bead based purification for rapid seamless purification• A 1 hour workflow• An input range of 1 5 µgThe RiboMinus Eukaryote System v2 improves upon the previous system RiboMinus Eukaryote Kit for RNA Seq by removing significantly more rRNA both cytoplasmic and mitochondrial from the total RNA When used with our library construction kits for sequencing the RiboMinus Eukaryote System v2 provides maximum mapping to reference database with minimum bias maintaining relative gene expression levels of even smaller RNA species The resulting total RNA after depletion contains minimum contaminating rRNA with reduced variability The RiboMinus Eukaryote System v2 is designed for the removal of human mouse and rat rRNA A magnetic bead based purification module is included for purification of the whole transcriptome without altering the expression levels of small or non coding RNA For column based purification please see the RiboMinus Eukaryote Kit v2 The RiboMinus Eukaryote System v2 has an RNA input range of 1 5 µg For lower inputs down to 100 ng the Low Input RiboMinus Eukaryote System v2 is recommended The RiboMinus Eukaryote System v2 contains sufficient reagents to perform 12 ribosomal RNA depletions
    https://www.bioz.com/result/ribominus eukaryote system v2/product/Thermo Fisher
    Average 99 stars, based on 34 article reviews
    Price from $9.99 to $1999.99
    ribominus eukaryote system v2 - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    RNA Sequencing Assay:

    Article Title: Sonic Hedgehog promotes proliferation of Notch-dependent monociliated choroid plexus tumour cells
    Article Snippet: .. For RNA-seq experiments, total RNA samples were ribo-depleted using the Ribominus Eukaryote System (Life Technologies), and used to generate sequencing libraries of barcoded fragment using the Ion Total RNA-Seq Kit V2 (Life Technologies). .. Libraries were sequenced on the Ion Proton sequencer, three libraries per Ion Proton PI Chip, using 200 bp sequencing reagents.

    Article Title: The mitochondrial transcriptome of the anglerfish Lophius piscatorius
    Article Snippet: .. Cellular rRNA was depleted from 1 μg of total RNA using the RiboMinus™ Eukaryote System v2 (Thermo Fisher Scientific, Waltham, MA—USA), and whole transcriptome library was constructed using the Ion Total RNA-seq kit v2 (Thermo Fisher Scientific) according to the manufacturers protocols. .. Manual template preparation on an Ion OneTouch™ 2 System (Thermo Fisher Scientific) and sequencing of two Ion 540™ chips on the Ion GeneStudio™ S5 System (Thermo Fisher Scientific) were carried out at our Genomics Platform (Nord University) according to the manufacturers protocols.

    Article Title: PAF Complex Plays Novel Subunit-Specific Roles in Alternative Cleavage and Polyadenylation
    Article Snippet: .. For RNA-seq, ribosomal RNA was removed using RiboMinus Eukaryote System v2 (Ambion) (Invitrogen, A15026), and strand-specific cDNA library preparation and sequencing were performed as described [ ]. ..

    Construct:

    Article Title: The mitochondrial transcriptome of the anglerfish Lophius piscatorius
    Article Snippet: .. Cellular rRNA was depleted from 1 μg of total RNA using the RiboMinus™ Eukaryote System v2 (Thermo Fisher Scientific, Waltham, MA—USA), and whole transcriptome library was constructed using the Ion Total RNA-seq kit v2 (Thermo Fisher Scientific) according to the manufacturers protocols. .. Manual template preparation on an Ion OneTouch™ 2 System (Thermo Fisher Scientific) and sequencing of two Ion 540™ chips on the Ion GeneStudio™ S5 System (Thermo Fisher Scientific) were carried out at our Genomics Platform (Nord University) according to the manufacturers protocols.

    Purification:

    Article Title: The Candida albicans Histone Acetyltransferase Hat1 Regulates Stress Resistance and Virulence via Distinct Chromatin Assembly Pathways
    Article Snippet: .. 5 μg DNase treated RNA were used for rRNA depletion with the RiboMinus Eukaryote System v2 (Life Technologies, Carlsbad, CA). rRNA depleted samples were fragmented using the NEBNext Magnesium RNA Fragmentation Module (New England Biolabs) and purified with the RNeasy MinElute Cleanup Kit (Qiagen). .. SuperScript III reverse transcriptase (Life Technologies, Carlsbad, CA) was used for first strand synthesis.

    Sequencing:

    Article Title: Sonic Hedgehog promotes proliferation of Notch-dependent monociliated choroid plexus tumour cells
    Article Snippet: .. For RNA-seq experiments, total RNA samples were ribo-depleted using the Ribominus Eukaryote System (Life Technologies), and used to generate sequencing libraries of barcoded fragment using the Ion Total RNA-Seq Kit V2 (Life Technologies). .. Libraries were sequenced on the Ion Proton sequencer, three libraries per Ion Proton PI Chip, using 200 bp sequencing reagents.

    Article Title: PAF Complex Plays Novel Subunit-Specific Roles in Alternative Cleavage and Polyadenylation
    Article Snippet: .. For RNA-seq, ribosomal RNA was removed using RiboMinus Eukaryote System v2 (Ambion) (Invitrogen, A15026), and strand-specific cDNA library preparation and sequencing were performed as described [ ]. ..

    other:

    Article Title: Combined short and long-read sequencing reveals a complex transcriptomic architecture of African swine fever virus
    Article Snippet: Removal of the ribosomal RNAsThe RiboMinus™ Eukaryote System v2 (Thermo Fisher Scientific) was used to obtain rRNA-free RNA samples which is required by the applied Illumina library preparation approach.

    cDNA Library Assay:

    Article Title: PAF Complex Plays Novel Subunit-Specific Roles in Alternative Cleavage and Polyadenylation
    Article Snippet: .. For RNA-seq, ribosomal RNA was removed using RiboMinus Eukaryote System v2 (Ambion) (Invitrogen, A15026), and strand-specific cDNA library preparation and sequencing were performed as described [ ]. ..

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  • 98
    Thermo Fisher low input ribominus eukaryote system v2
    Identification of SARS-Cov-2 through nasopharyngeal swab metatranscriptomics. A) Left panel: extraction of RNA from nasopharyngeal swab was performed according to a protocol validated in our laboratory (COVID-19 Extraction and Amplification with Maxwell® 16 Viral Total Nucleic Acid and GoTaq® Probe 1-Step RT-qPCR; Application Report, Promega 2020). Total RNA sequencing after rRNA depletion followed the protocol of the Ion Total RNA-Seq kit v2, in the Ion S5 platform. Depletion of human ribosomal RNA was done with the Low Input <t>RiboMinus™</t> Eukaryote <t>System</t> v2 kit. Right panel: raw reads were submitted to quality filter where reads larger than 30 nt with Phred quality > 20 were aligned into human reference genome. Unaligned reads were used to perform contig assemblage. Assembled contigs were compared to NCBI databases using Blast software. Contigs that presented sequence similarity to SARS-Cov2-2 with e-value lower than 1e-5 were considered as from viral origin. Viral contigs were further submitted to contig extension using SPAdes and ‘trusted contigs’ option that was followed by cap3 tool to remove sequence redundancy. B) SARS-Cov-2 coverage profile of reads and assembled contigs. Reads were normalized by number of reads from each library. Contigs in red indicate high quality contigs larger than 400 nt with coverage of reads in both libraries.
    Low Input Ribominus Eukaryote System V2, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/low input ribominus eukaryote system v2/product/Thermo Fisher
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    low input ribominus eukaryote system v2 - by Bioz Stars, 2020-09
    98/100 stars
      Buy from Supplier

    97
    Thermo Fisher ribominus eukaryote kit v2
    Identification of SARS-Cov-2 through nasopharyngeal swab metatranscriptomics. A) Left panel: extraction of RNA from nasopharyngeal swab was performed according to a protocol validated in our laboratory (COVID-19 Extraction and Amplification with Maxwell® 16 Viral Total Nucleic Acid and GoTaq® Probe 1-Step RT-qPCR; Application Report, Promega 2020). Total RNA sequencing after rRNA depletion followed the protocol of the Ion Total RNA-Seq kit v2, in the Ion S5 platform. Depletion of human ribosomal RNA was done with the Low Input <t>RiboMinus™</t> Eukaryote <t>System</t> v2 kit. Right panel: raw reads were submitted to quality filter where reads larger than 30 nt with Phred quality > 20 were aligned into human reference genome. Unaligned reads were used to perform contig assemblage. Assembled contigs were compared to NCBI databases using Blast software. Contigs that presented sequence similarity to SARS-Cov2-2 with e-value lower than 1e-5 were considered as from viral origin. Viral contigs were further submitted to contig extension using SPAdes and ‘trusted contigs’ option that was followed by cap3 tool to remove sequence redundancy. B) SARS-Cov-2 coverage profile of reads and assembled contigs. Reads were normalized by number of reads from each library. Contigs in red indicate high quality contigs larger than 400 nt with coverage of reads in both libraries.
    Ribominus Eukaryote Kit V2, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ribominus eukaryote kit v2/product/Thermo Fisher
    Average 97 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    ribominus eukaryote kit v2 - by Bioz Stars, 2020-09
    97/100 stars
      Buy from Supplier

    Image Search Results


    Identification of SARS-Cov-2 through nasopharyngeal swab metatranscriptomics. A) Left panel: extraction of RNA from nasopharyngeal swab was performed according to a protocol validated in our laboratory (COVID-19 Extraction and Amplification with Maxwell® 16 Viral Total Nucleic Acid and GoTaq® Probe 1-Step RT-qPCR; Application Report, Promega 2020). Total RNA sequencing after rRNA depletion followed the protocol of the Ion Total RNA-Seq kit v2, in the Ion S5 platform. Depletion of human ribosomal RNA was done with the Low Input RiboMinus™ Eukaryote System v2 kit. Right panel: raw reads were submitted to quality filter where reads larger than 30 nt with Phred quality > 20 were aligned into human reference genome. Unaligned reads were used to perform contig assemblage. Assembled contigs were compared to NCBI databases using Blast software. Contigs that presented sequence similarity to SARS-Cov2-2 with e-value lower than 1e-5 were considered as from viral origin. Viral contigs were further submitted to contig extension using SPAdes and ‘trusted contigs’ option that was followed by cap3 tool to remove sequence redundancy. B) SARS-Cov-2 coverage profile of reads and assembled contigs. Reads were normalized by number of reads from each library. Contigs in red indicate high quality contigs larger than 400 nt with coverage of reads in both libraries.

    Journal: Journal of Virological Methods

    Article Title: Ion torrent-based nasopharyngeal swab metatranscriptomics in COVID-19

    doi: 10.1016/j.jviromet.2020.113888

    Figure Lengend Snippet: Identification of SARS-Cov-2 through nasopharyngeal swab metatranscriptomics. A) Left panel: extraction of RNA from nasopharyngeal swab was performed according to a protocol validated in our laboratory (COVID-19 Extraction and Amplification with Maxwell® 16 Viral Total Nucleic Acid and GoTaq® Probe 1-Step RT-qPCR; Application Report, Promega 2020). Total RNA sequencing after rRNA depletion followed the protocol of the Ion Total RNA-Seq kit v2, in the Ion S5 platform. Depletion of human ribosomal RNA was done with the Low Input RiboMinus™ Eukaryote System v2 kit. Right panel: raw reads were submitted to quality filter where reads larger than 30 nt with Phred quality > 20 were aligned into human reference genome. Unaligned reads were used to perform contig assemblage. Assembled contigs were compared to NCBI databases using Blast software. Contigs that presented sequence similarity to SARS-Cov2-2 with e-value lower than 1e-5 were considered as from viral origin. Viral contigs were further submitted to contig extension using SPAdes and ‘trusted contigs’ option that was followed by cap3 tool to remove sequence redundancy. B) SARS-Cov-2 coverage profile of reads and assembled contigs. Reads were normalized by number of reads from each library. Contigs in red indicate high quality contigs larger than 400 nt with coverage of reads in both libraries.

    Article Snippet: One sample was previously processed with the Low Input RiboMinus™ Eukaryote System v2 (ThermoFisher), for depletion of human ribosomal RNA from total RNA ( ).

    Techniques: Amplification, Quantitative RT-PCR, RNA Sequencing Assay, Software, Sequencing