Journal: Biology

Article Title: Messenger RNA Gene Expression Screening of VIP and PACAP Neuropeptides and Their Endogenous Receptors in Ruminants

doi: 10.3390/biology11101512

Figure Lengend Snippet: MIQE Checklist. Descriptions of each parameter of the MIQE Checklist. E = All essential information. D = Desired Information that is submitted if available.

Article Snippet: Complete reaction conditions , E , ✓ , cDNA reactions containing final concentrations of FIREScript RT (10 U/µL), RiboGripTM RNase Inhibitor (1 U/µL), dNTP MIX (500 µM of each), Oligo dT (2.5 µM) Random primers (2.5 µM) and 1x RT Reaction Buffer with DTT with final concentrations of 50 mM Tris-HCl pH 8.3, 50 mM KCl, 3 mM MgCl2, 10 mM DTT.

Techniques: Produced, Laser Capture Microdissection, Fluorescence, Spectrophotometry, Electrophoresis, Inhibition, Concentration Assay, Incubation, Negative Control, Multiplex Assay, Sequencing, Amplification, In Silico, Purification, SYBR Green Assay, Software, Expressing, Intra Assay, Inter Assay