rhodamine  (Vector Laboratories)


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    Vector Laboratories rhodamine
    Rhodamine, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rhodamine  (Vector Laboratories)


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    Vector Laboratories rhodamine
    Rhodamine, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rhodamine ricinus communis agglutinin i rca  (Vector Laboratories)


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    Vector Laboratories rhodamine ricinus communis agglutinin i rca
    Rhodamine Ricinus Communis Agglutinin I Rca, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ricinus communis agglutinin i rca 1 lectins  (Vector Laboratories)


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    Vector Laboratories ricinus communis agglutinin i rca 1 lectins
    Viperid snake venoms induces platelet desialylation. ( A , B ) Effect of the venoms from D. siamensis (DSV), A. halys (AHV) and B. multicinctus (BMV) on <t>RCA-1</t> binding. Effect of OSGE and oseltamivir on ( C ) DSV and ( D ) AHV venoms induced RCA-1 binding. Statistical analysis was performed with the one-way ANOVA test. n = 3. *** p < 0.001.
    Ricinus Communis Agglutinin I Rca 1 Lectins, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 1 article reviews
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    ricinus communis agglutinin i rca 1 lectins - by Bioz Stars, 2024-07
    93/100 stars

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    1) Product Images from "Platelet Desialylation Is a Novel Mechanism and Therapeutic Target in Daboia siamensis and Agkistrodon halys Envenomation-Induced Thrombocytopenia"

    Article Title: Platelet Desialylation Is a Novel Mechanism and Therapeutic Target in Daboia siamensis and Agkistrodon halys Envenomation-Induced Thrombocytopenia

    Journal: Molecules

    doi: 10.3390/molecules27227779

    Viperid snake venoms induces platelet desialylation. ( A , B ) Effect of the venoms from D. siamensis (DSV), A. halys (AHV) and B. multicinctus (BMV) on RCA-1 binding. Effect of OSGE and oseltamivir on ( C ) DSV and ( D ) AHV venoms induced RCA-1 binding. Statistical analysis was performed with the one-way ANOVA test. n = 3. *** p < 0.001.
    Figure Legend Snippet: Viperid snake venoms induces platelet desialylation. ( A , B ) Effect of the venoms from D. siamensis (DSV), A. halys (AHV) and B. multicinctus (BMV) on RCA-1 binding. Effect of OSGE and oseltamivir on ( C ) DSV and ( D ) AHV venoms induced RCA-1 binding. Statistical analysis was performed with the one-way ANOVA test. n = 3. *** p < 0.001.

    Techniques Used: Binding Assay

    rhodamine lectin  (Vector Laboratories)


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    Vector Laboratories rhodamine lectin
    Rhodamine Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ricinus communis agglutinin i rca i  (Vector Laboratories)


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    Vector Laboratories ricinus communis agglutinin i rca i
    Ricinus Communis Agglutinin I Rca I, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rca i immunohistochemistry  (Vector Laboratories)


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    Vector Laboratories rca i immunohistochemistry
    Rca I Immunohistochemistry, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rhodamine labelled ricinus communis agglutinin  (Vector Laboratories)


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    Vector Laboratories rhodamine labelled ricinus communis agglutinin
    Rhodamine Labelled Ricinus Communis Agglutinin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ricinus communis agglutinin i  (Vector Laboratories)


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    Vector Laboratories ricinus communis agglutinin i
    Abnormal flow pattern of fluorescent tracer in ADN–VEGF-C mice. Fluorescence stereomicroscopic images of Prox1-GFP control mouse (A) and ADN–VEGF-C/Prox1-GFP mouse on doxycycline for 7 days (B) showing lymphatic vessels (green) 5 minutes after injection of rhodamine-labeled Ricinus communis <t>agglutinin</t> <t>I</t> (RCA I) lectin tracer (red) into a mesenteric lymph node. Image orientation: caudal (left), rostral (right). Arrows mark tracer in paravertebral lymphatic vessel. n = 5 mice (A and B). Scale bar = 1 mm (A and B).
    Ricinus Communis Agglutinin I, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ricinus communis agglutinin i - by Bioz Stars, 2024-07
    93/100 stars

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    1) Product Images from "Retrograde Lymph Flow Leads to Chylothorax in Transgenic Mice with Lymphatic Malformations"

    Article Title: Retrograde Lymph Flow Leads to Chylothorax in Transgenic Mice with Lymphatic Malformations

    Journal: The American Journal of Pathology

    doi: 10.1016/j.ajpath.2017.05.009

    Abnormal flow pattern of fluorescent tracer in ADN–VEGF-C mice. Fluorescence stereomicroscopic images of Prox1-GFP control mouse (A) and ADN–VEGF-C/Prox1-GFP mouse on doxycycline for 7 days (B) showing lymphatic vessels (green) 5 minutes after injection of rhodamine-labeled Ricinus communis agglutinin I (RCA I) lectin tracer (red) into a mesenteric lymph node. Image orientation: caudal (left), rostral (right). Arrows mark tracer in paravertebral lymphatic vessel. n = 5 mice (A and B). Scale bar = 1 mm (A and B).
    Figure Legend Snippet: Abnormal flow pattern of fluorescent tracer in ADN–VEGF-C mice. Fluorescence stereomicroscopic images of Prox1-GFP control mouse (A) and ADN–VEGF-C/Prox1-GFP mouse on doxycycline for 7 days (B) showing lymphatic vessels (green) 5 minutes after injection of rhodamine-labeled Ricinus communis agglutinin I (RCA I) lectin tracer (red) into a mesenteric lymph node. Image orientation: caudal (left), rostral (right). Arrows mark tracer in paravertebral lymphatic vessel. n = 5 mice (A and B). Scale bar = 1 mm (A and B).

    Techniques Used: Fluorescence, Injection, Labeling

    Retrograde fluorescent tracer flow into lymphatic plexuses and thoracic cavity. Image orientation: caudal (left), rostral (right). A: Bright-field image of region captured by time-lapse microscopic imaging. B: First frame at 3 seconds (left panel) after start of injection and last frame at 120 seconds (right panel) of a time-lapse video showing fluorescent Ricinus communis agglutinin I (RCA I) lectin (white) confined to thoracic duct of control mouse. C: Time-lapse video frames of ADN–VEGF-C mouse showing rapid movement of tracer out of thoracic duct (9 seconds), into adjacent lymphatics (22 seconds), and leakage into the thoracic cavity (45 and 120 seconds). Arrowheads mark diffuse extravasated tracer. D: Fluorescent lectin was confined to thoracic duct (blue), and not present in paravertebral lymphatics, in 100% of six single transgenic control mice, but was found in paravertebral lymphatics (red) within 6 seconds (two frames) after reaching the thoracic duct of 26 (90%) of 29 ADN–VEGF-C mice. E: Images of fluorescent tracer leakage before (left panel, 120 seconds after injection) and after (right panel) PBS wash of pleural surface showing that some tracer was removed (arrowheads), consistent with entry into the thoracic cavity. n = 6 mice (E). Scale bar = 2 mm (A–C and E).
    Figure Legend Snippet: Retrograde fluorescent tracer flow into lymphatic plexuses and thoracic cavity. Image orientation: caudal (left), rostral (right). A: Bright-field image of region captured by time-lapse microscopic imaging. B: First frame at 3 seconds (left panel) after start of injection and last frame at 120 seconds (right panel) of a time-lapse video showing fluorescent Ricinus communis agglutinin I (RCA I) lectin (white) confined to thoracic duct of control mouse. C: Time-lapse video frames of ADN–VEGF-C mouse showing rapid movement of tracer out of thoracic duct (9 seconds), into adjacent lymphatics (22 seconds), and leakage into the thoracic cavity (45 and 120 seconds). Arrowheads mark diffuse extravasated tracer. D: Fluorescent lectin was confined to thoracic duct (blue), and not present in paravertebral lymphatics, in 100% of six single transgenic control mice, but was found in paravertebral lymphatics (red) within 6 seconds (two frames) after reaching the thoracic duct of 26 (90%) of 29 ADN–VEGF-C mice. E: Images of fluorescent tracer leakage before (left panel, 120 seconds after injection) and after (right panel) PBS wash of pleural surface showing that some tracer was removed (arrowheads), consistent with entry into the thoracic cavity. n = 6 mice (E). Scale bar = 2 mm (A–C and E).

    Techniques Used: Imaging, Injection, Transgenic Assay

    Coincidence of subpleural lymphatic plexuses and leakage sites. Image orientation: caudal (left), rostral (right). ADN–VEGF-C mice were analyzed after 0.075 mg/mL doxycycline for 7 days. A: Left panel: Whole mount of dorsal surface of thorax of Prox1-GFP control mouse after horseradish peroxidase (HRP) staining for GFP. Right panel: Drawing of whole mount where green lines show GFP+ lymphatic vessels [thoracic duct (TD), paravertebral lymphatic vessel (PVL), and lymphatic plexus (LP)]. Gray lines mark the location of ribs. B: Paravertebral lymphatic vessel (arrows indicate valves) and connecting lymphatic (arrowheads indicate valves) to subpleural lymphatic plexuses. C: Fluorescence stereomicroscopic image of the pleural surface of the chest wall of a 3-week-old Prox1-GFP mouse showing lymphatic vessels (green; arrowheads) that connect the TD to PVLs. Some regions of the connecting lymphatics (arrow) are obscured by overlying tissue. D: Images of a region of chest wall showing sites of tracer leakage (red; left panel), LYVE-1–HRP staining of lymphatic plexuses (brown; middle panel), and an overlay of the two (white; LYVE-1–HRP; right panel). E: Enlarged images of lymphatic plexuses (LYVE-1–HRP) in chest wall of single transgenic control mouse and ADN–VEGF-C mouse, the latter showing bud-like outgrowths (arrowheads). F: Regional area per lymphatic plexus in single-transgenic and ADN–VEGF-C mice. Each dot shows the area of one plexus. Red lines indicate plexus area means. n = 7 mice (D); n = 4 mice per group (E and F). ∗∗P < 0.01 (t-test). Scale bars: 4 mm (A); 1 mm (B–E). RCA I, Ricinus communis agglutinin I.
    Figure Legend Snippet: Coincidence of subpleural lymphatic plexuses and leakage sites. Image orientation: caudal (left), rostral (right). ADN–VEGF-C mice were analyzed after 0.075 mg/mL doxycycline for 7 days. A: Left panel: Whole mount of dorsal surface of thorax of Prox1-GFP control mouse after horseradish peroxidase (HRP) staining for GFP. Right panel: Drawing of whole mount where green lines show GFP+ lymphatic vessels [thoracic duct (TD), paravertebral lymphatic vessel (PVL), and lymphatic plexus (LP)]. Gray lines mark the location of ribs. B: Paravertebral lymphatic vessel (arrows indicate valves) and connecting lymphatic (arrowheads indicate valves) to subpleural lymphatic plexuses. C: Fluorescence stereomicroscopic image of the pleural surface of the chest wall of a 3-week-old Prox1-GFP mouse showing lymphatic vessels (green; arrowheads) that connect the TD to PVLs. Some regions of the connecting lymphatics (arrow) are obscured by overlying tissue. D: Images of a region of chest wall showing sites of tracer leakage (red; left panel), LYVE-1–HRP staining of lymphatic plexuses (brown; middle panel), and an overlay of the two (white; LYVE-1–HRP; right panel). E: Enlarged images of lymphatic plexuses (LYVE-1–HRP) in chest wall of single transgenic control mouse and ADN–VEGF-C mouse, the latter showing bud-like outgrowths (arrowheads). F: Regional area per lymphatic plexus in single-transgenic and ADN–VEGF-C mice. Each dot shows the area of one plexus. Red lines indicate plexus area means. n = 7 mice (D); n = 4 mice per group (E and F). ∗∗P < 0.01 (t-test). Scale bars: 4 mm (A); 1 mm (B–E). RCA I, Ricinus communis agglutinin I.

    Techniques Used: Staining, Fluorescence, Transgenic Assay

    rhodamine ricinus communis agglutinin i  (Vector Laboratories)


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    Vector Laboratories rhodamine ricinus communis agglutinin i
    Rhodamine Ricinus Communis Agglutinin I, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rhodamine labeled ricinus communis agglutinin  (Vector Laboratories)


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    Vector Laboratories rhodamine labeled ricinus communis agglutinin
    Rhodamine Labeled Ricinus Communis Agglutinin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vector Laboratories ricinus communis agglutinin i rca 1 lectins
    Viperid snake venoms induces platelet desialylation. ( A , B ) Effect of the venoms from D. siamensis (DSV), A. halys (AHV) and B. multicinctus (BMV) on <t>RCA-1</t> binding. Effect of OSGE and oseltamivir on ( C ) DSV and ( D ) AHV venoms induced RCA-1 binding. Statistical analysis was performed with the one-way ANOVA test. n = 3. *** p < 0.001.
    Ricinus Communis Agglutinin I Rca 1 Lectins, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Viperid snake venoms induces platelet desialylation. ( A , B ) Effect of the venoms from D. siamensis (DSV), A. halys (AHV) and B. multicinctus (BMV) on <t>RCA-1</t> binding. Effect of OSGE and oseltamivir on ( C ) DSV and ( D ) AHV venoms induced RCA-1 binding. Statistical analysis was performed with the one-way ANOVA test. n = 3. *** p < 0.001.
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    Viperid snake venoms induces platelet desialylation. ( A , B ) Effect of the venoms from D. siamensis (DSV), A. halys (AHV) and B. multicinctus (BMV) on <t>RCA-1</t> binding. Effect of OSGE and oseltamivir on ( C ) DSV and ( D ) AHV venoms induced RCA-1 binding. Statistical analysis was performed with the one-way ANOVA test. n = 3. *** p < 0.001.
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    Vector Laboratories rhodamine labelled ricinus communis agglutinin
    Viperid snake venoms induces platelet desialylation. ( A , B ) Effect of the venoms from D. siamensis (DSV), A. halys (AHV) and B. multicinctus (BMV) on <t>RCA-1</t> binding. Effect of OSGE and oseltamivir on ( C ) DSV and ( D ) AHV venoms induced RCA-1 binding. Statistical analysis was performed with the one-way ANOVA test. n = 3. *** p < 0.001.
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    Abnormal flow pattern of fluorescent tracer in ADN–VEGF-C mice. Fluorescence stereomicroscopic images of Prox1-GFP control mouse (A) and ADN–VEGF-C/Prox1-GFP mouse on doxycycline for 7 days (B) showing lymphatic vessels (green) 5 minutes after injection of rhodamine-labeled Ricinus communis <t>agglutinin</t> <t>I</t> (RCA I) lectin tracer (red) into a mesenteric lymph node. Image orientation: caudal (left), rostral (right). Arrows mark tracer in paravertebral lymphatic vessel. n = 5 mice (A and B). Scale bar = 1 mm (A and B).
    Ricinus Communis Agglutinin I, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vector Laboratories rhodamine ricinus communis agglutinin i
    Abnormal flow pattern of fluorescent tracer in ADN–VEGF-C mice. Fluorescence stereomicroscopic images of Prox1-GFP control mouse (A) and ADN–VEGF-C/Prox1-GFP mouse on doxycycline for 7 days (B) showing lymphatic vessels (green) 5 minutes after injection of rhodamine-labeled Ricinus communis <t>agglutinin</t> <t>I</t> (RCA I) lectin tracer (red) into a mesenteric lymph node. Image orientation: caudal (left), rostral (right). Arrows mark tracer in paravertebral lymphatic vessel. n = 5 mice (A and B). Scale bar = 1 mm (A and B).
    Rhodamine Ricinus Communis Agglutinin I, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vector Laboratories rhodamine labeled ricinus communis agglutinin
    Abnormal flow pattern of fluorescent tracer in ADN–VEGF-C mice. Fluorescence stereomicroscopic images of Prox1-GFP control mouse (A) and ADN–VEGF-C/Prox1-GFP mouse on doxycycline for 7 days (B) showing lymphatic vessels (green) 5 minutes after injection of rhodamine-labeled Ricinus communis <t>agglutinin</t> <t>I</t> (RCA I) lectin tracer (red) into a mesenteric lymph node. Image orientation: caudal (left), rostral (right). Arrows mark tracer in paravertebral lymphatic vessel. n = 5 mice (A and B). Scale bar = 1 mm (A and B).
    Rhodamine Labeled Ricinus Communis Agglutinin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Viperid snake venoms induces platelet desialylation. ( A , B ) Effect of the venoms from D. siamensis (DSV), A. halys (AHV) and B. multicinctus (BMV) on RCA-1 binding. Effect of OSGE and oseltamivir on ( C ) DSV and ( D ) AHV venoms induced RCA-1 binding. Statistical analysis was performed with the one-way ANOVA test. n = 3. *** p < 0.001.

    Journal: Molecules

    Article Title: Platelet Desialylation Is a Novel Mechanism and Therapeutic Target in Daboia siamensis and Agkistrodon halys Envenomation-Induced Thrombocytopenia

    doi: 10.3390/molecules27227779

    Figure Lengend Snippet: Viperid snake venoms induces platelet desialylation. ( A , B ) Effect of the venoms from D. siamensis (DSV), A. halys (AHV) and B. multicinctus (BMV) on RCA-1 binding. Effect of OSGE and oseltamivir on ( C ) DSV and ( D ) AHV venoms induced RCA-1 binding. Statistical analysis was performed with the one-way ANOVA test. n = 3. *** p < 0.001.

    Article Snippet: Platelet desialylation level was detected by rhodamine-labeled ricinus communis agglutinin I (RCA-1) lectins (1/1000 dilution, RL-1082-5, VECTOR, Burlingame, CA, USA), which specifically binds to the galactose residues caused by glycoprotein desialylation [ , ].

    Techniques: Binding Assay

    Abnormal flow pattern of fluorescent tracer in ADN–VEGF-C mice. Fluorescence stereomicroscopic images of Prox1-GFP control mouse (A) and ADN–VEGF-C/Prox1-GFP mouse on doxycycline for 7 days (B) showing lymphatic vessels (green) 5 minutes after injection of rhodamine-labeled Ricinus communis agglutinin I (RCA I) lectin tracer (red) into a mesenteric lymph node. Image orientation: caudal (left), rostral (right). Arrows mark tracer in paravertebral lymphatic vessel. n = 5 mice (A and B). Scale bar = 1 mm (A and B).

    Journal: The American Journal of Pathology

    Article Title: Retrograde Lymph Flow Leads to Chylothorax in Transgenic Mice with Lymphatic Malformations

    doi: 10.1016/j.ajpath.2017.05.009

    Figure Lengend Snippet: Abnormal flow pattern of fluorescent tracer in ADN–VEGF-C mice. Fluorescence stereomicroscopic images of Prox1-GFP control mouse (A) and ADN–VEGF-C/Prox1-GFP mouse on doxycycline for 7 days (B) showing lymphatic vessels (green) 5 minutes after injection of rhodamine-labeled Ricinus communis agglutinin I (RCA I) lectin tracer (red) into a mesenteric lymph node. Image orientation: caudal (left), rostral (right). Arrows mark tracer in paravertebral lymphatic vessel. n = 5 mice (A and B). Scale bar = 1 mm (A and B).

    Article Snippet: Two minutes later, 40 μL of a solution containing 1.25 mg/mL rhodamine-labeled Ricinus communis agglutinin I (Vector; RL-1082) and 0.9% sodium chloride was injected into a mesenteric lymph node through a 27-gauge butterfly needle with 8-inch tubing (Abbott Laboratories, Lake Bluff, IL) at 40 to 50 mm Hg.

    Techniques: Fluorescence, Injection, Labeling

    Retrograde fluorescent tracer flow into lymphatic plexuses and thoracic cavity. Image orientation: caudal (left), rostral (right). A: Bright-field image of region captured by time-lapse microscopic imaging. B: First frame at 3 seconds (left panel) after start of injection and last frame at 120 seconds (right panel) of a time-lapse video showing fluorescent Ricinus communis agglutinin I (RCA I) lectin (white) confined to thoracic duct of control mouse. C: Time-lapse video frames of ADN–VEGF-C mouse showing rapid movement of tracer out of thoracic duct (9 seconds), into adjacent lymphatics (22 seconds), and leakage into the thoracic cavity (45 and 120 seconds). Arrowheads mark diffuse extravasated tracer. D: Fluorescent lectin was confined to thoracic duct (blue), and not present in paravertebral lymphatics, in 100% of six single transgenic control mice, but was found in paravertebral lymphatics (red) within 6 seconds (two frames) after reaching the thoracic duct of 26 (90%) of 29 ADN–VEGF-C mice. E: Images of fluorescent tracer leakage before (left panel, 120 seconds after injection) and after (right panel) PBS wash of pleural surface showing that some tracer was removed (arrowheads), consistent with entry into the thoracic cavity. n = 6 mice (E). Scale bar = 2 mm (A–C and E).

    Journal: The American Journal of Pathology

    Article Title: Retrograde Lymph Flow Leads to Chylothorax in Transgenic Mice with Lymphatic Malformations

    doi: 10.1016/j.ajpath.2017.05.009

    Figure Lengend Snippet: Retrograde fluorescent tracer flow into lymphatic plexuses and thoracic cavity. Image orientation: caudal (left), rostral (right). A: Bright-field image of region captured by time-lapse microscopic imaging. B: First frame at 3 seconds (left panel) after start of injection and last frame at 120 seconds (right panel) of a time-lapse video showing fluorescent Ricinus communis agglutinin I (RCA I) lectin (white) confined to thoracic duct of control mouse. C: Time-lapse video frames of ADN–VEGF-C mouse showing rapid movement of tracer out of thoracic duct (9 seconds), into adjacent lymphatics (22 seconds), and leakage into the thoracic cavity (45 and 120 seconds). Arrowheads mark diffuse extravasated tracer. D: Fluorescent lectin was confined to thoracic duct (blue), and not present in paravertebral lymphatics, in 100% of six single transgenic control mice, but was found in paravertebral lymphatics (red) within 6 seconds (two frames) after reaching the thoracic duct of 26 (90%) of 29 ADN–VEGF-C mice. E: Images of fluorescent tracer leakage before (left panel, 120 seconds after injection) and after (right panel) PBS wash of pleural surface showing that some tracer was removed (arrowheads), consistent with entry into the thoracic cavity. n = 6 mice (E). Scale bar = 2 mm (A–C and E).

    Article Snippet: Two minutes later, 40 μL of a solution containing 1.25 mg/mL rhodamine-labeled Ricinus communis agglutinin I (Vector; RL-1082) and 0.9% sodium chloride was injected into a mesenteric lymph node through a 27-gauge butterfly needle with 8-inch tubing (Abbott Laboratories, Lake Bluff, IL) at 40 to 50 mm Hg.

    Techniques: Imaging, Injection, Transgenic Assay

    Coincidence of subpleural lymphatic plexuses and leakage sites. Image orientation: caudal (left), rostral (right). ADN–VEGF-C mice were analyzed after 0.075 mg/mL doxycycline for 7 days. A: Left panel: Whole mount of dorsal surface of thorax of Prox1-GFP control mouse after horseradish peroxidase (HRP) staining for GFP. Right panel: Drawing of whole mount where green lines show GFP+ lymphatic vessels [thoracic duct (TD), paravertebral lymphatic vessel (PVL), and lymphatic plexus (LP)]. Gray lines mark the location of ribs. B: Paravertebral lymphatic vessel (arrows indicate valves) and connecting lymphatic (arrowheads indicate valves) to subpleural lymphatic plexuses. C: Fluorescence stereomicroscopic image of the pleural surface of the chest wall of a 3-week-old Prox1-GFP mouse showing lymphatic vessels (green; arrowheads) that connect the TD to PVLs. Some regions of the connecting lymphatics (arrow) are obscured by overlying tissue. D: Images of a region of chest wall showing sites of tracer leakage (red; left panel), LYVE-1–HRP staining of lymphatic plexuses (brown; middle panel), and an overlay of the two (white; LYVE-1–HRP; right panel). E: Enlarged images of lymphatic plexuses (LYVE-1–HRP) in chest wall of single transgenic control mouse and ADN–VEGF-C mouse, the latter showing bud-like outgrowths (arrowheads). F: Regional area per lymphatic plexus in single-transgenic and ADN–VEGF-C mice. Each dot shows the area of one plexus. Red lines indicate plexus area means. n = 7 mice (D); n = 4 mice per group (E and F). ∗∗P < 0.01 (t-test). Scale bars: 4 mm (A); 1 mm (B–E). RCA I, Ricinus communis agglutinin I.

    Journal: The American Journal of Pathology

    Article Title: Retrograde Lymph Flow Leads to Chylothorax in Transgenic Mice with Lymphatic Malformations

    doi: 10.1016/j.ajpath.2017.05.009

    Figure Lengend Snippet: Coincidence of subpleural lymphatic plexuses and leakage sites. Image orientation: caudal (left), rostral (right). ADN–VEGF-C mice were analyzed after 0.075 mg/mL doxycycline for 7 days. A: Left panel: Whole mount of dorsal surface of thorax of Prox1-GFP control mouse after horseradish peroxidase (HRP) staining for GFP. Right panel: Drawing of whole mount where green lines show GFP+ lymphatic vessels [thoracic duct (TD), paravertebral lymphatic vessel (PVL), and lymphatic plexus (LP)]. Gray lines mark the location of ribs. B: Paravertebral lymphatic vessel (arrows indicate valves) and connecting lymphatic (arrowheads indicate valves) to subpleural lymphatic plexuses. C: Fluorescence stereomicroscopic image of the pleural surface of the chest wall of a 3-week-old Prox1-GFP mouse showing lymphatic vessels (green; arrowheads) that connect the TD to PVLs. Some regions of the connecting lymphatics (arrow) are obscured by overlying tissue. D: Images of a region of chest wall showing sites of tracer leakage (red; left panel), LYVE-1–HRP staining of lymphatic plexuses (brown; middle panel), and an overlay of the two (white; LYVE-1–HRP; right panel). E: Enlarged images of lymphatic plexuses (LYVE-1–HRP) in chest wall of single transgenic control mouse and ADN–VEGF-C mouse, the latter showing bud-like outgrowths (arrowheads). F: Regional area per lymphatic plexus in single-transgenic and ADN–VEGF-C mice. Each dot shows the area of one plexus. Red lines indicate plexus area means. n = 7 mice (D); n = 4 mice per group (E and F). ∗∗P < 0.01 (t-test). Scale bars: 4 mm (A); 1 mm (B–E). RCA I, Ricinus communis agglutinin I.

    Article Snippet: Two minutes later, 40 μL of a solution containing 1.25 mg/mL rhodamine-labeled Ricinus communis agglutinin I (Vector; RL-1082) and 0.9% sodium chloride was injected into a mesenteric lymph node through a 27-gauge butterfly needle with 8-inch tubing (Abbott Laboratories, Lake Bluff, IL) at 40 to 50 mm Hg.

    Techniques: Staining, Fluorescence, Transgenic Assay