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anti rhoa gtp curiecoretech  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc anti rhoa gtp curiecoretech
    a,b Immunofluorescence images of Gα 13 WT-YFP, Gα 13 R200K-YFP or Gα 13 Q226L-YFP (green) expressed in NHEM. Left panels: The cells were labelled with phalloidin for visualising F-actin ( a ) or pMLC ( b ) (red), and Hoechst for nuclei (blue). Right panels: Quantifications of morphological parameters (perimeter, circularity and solidity) and F-actin content ( a ), and pMLC levels ( b ). c Quantification of <t>RHOA-GTP</t> levels in Gα 13 WT-YFP, Gα 13 R200K-YFP or Gα 13 Q226L-YFP -expressing NHEM cells. Means +/- SEM are shown.
    Anti Rhoa Gtp Curiecoretech, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti rhoa gtp curiecoretech/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti rhoa gtp curiecoretech - by Bioz Stars, 2024-11
    86/100 stars

    Images

    1) Product Images from "A postzygotic GNA13 variant upregulates the RHOA/ROCK pathway and alters melanocyte function in a mosaic skin hypopigmentation syndrome"

    Article Title: A postzygotic GNA13 variant upregulates the RHOA/ROCK pathway and alters melanocyte function in a mosaic skin hypopigmentation syndrome

    Journal: medRxiv

    doi: 10.1101/2024.07.24.24310661

    a,b Immunofluorescence images of Gα 13 WT-YFP, Gα 13 R200K-YFP or Gα 13 Q226L-YFP (green) expressed in NHEM. Left panels: The cells were labelled with phalloidin for visualising F-actin ( a ) or pMLC ( b ) (red), and Hoechst for nuclei (blue). Right panels: Quantifications of morphological parameters (perimeter, circularity and solidity) and F-actin content ( a ), and pMLC levels ( b ). c Quantification of RHOA-GTP levels in Gα 13 WT-YFP, Gα 13 R200K-YFP or Gα 13 Q226L-YFP -expressing NHEM cells. Means +/- SEM are shown.
    Figure Legend Snippet: a,b Immunofluorescence images of Gα 13 WT-YFP, Gα 13 R200K-YFP or Gα 13 Q226L-YFP (green) expressed in NHEM. Left panels: The cells were labelled with phalloidin for visualising F-actin ( a ) or pMLC ( b ) (red), and Hoechst for nuclei (blue). Right panels: Quantifications of morphological parameters (perimeter, circularity and solidity) and F-actin content ( a ), and pMLC levels ( b ). c Quantification of RHOA-GTP levels in Gα 13 WT-YFP, Gα 13 R200K-YFP or Gα 13 Q226L-YFP -expressing NHEM cells. Means +/- SEM are shown.

    Techniques Used: Immunofluorescence, Expressing

    a Quantification of RHOA-GTP levels in Gα 13 WT-YFP, Gα 13 R200K-YFP or Gα 13 Q226L-YFP expressing B16-F0 cells that correspond to the mean intensity of the signal generated from anti-GTP RHOA antibody labelling. Quantification of F-actin content ( b ) and circularity ( c ) in RHOA WT-GFP and RHOA Q63L-GFP expressing B16-F0 cells. Quantification of F-actin content ( d ) and circularity ( e ) in Gα 13 WT-YFP, Gα 13 R200K-YFP or Gα 13 Q226L-YFP expressing B16-F0 cells, upon RHOA inhibition treatment with CT04. Quantification of F-actin content ( f ) and circularity ( g ) in Gα 13 WT-YFP, Gα 13 R200K-YFP or Gα 13 Q226L-YFP expressing B16-F0 cells, upon ROCK inhibition treatment with Y27632. Means +/- SEM are shown.
    Figure Legend Snippet: a Quantification of RHOA-GTP levels in Gα 13 WT-YFP, Gα 13 R200K-YFP or Gα 13 Q226L-YFP expressing B16-F0 cells that correspond to the mean intensity of the signal generated from anti-GTP RHOA antibody labelling. Quantification of F-actin content ( b ) and circularity ( c ) in RHOA WT-GFP and RHOA Q63L-GFP expressing B16-F0 cells. Quantification of F-actin content ( d ) and circularity ( e ) in Gα 13 WT-YFP, Gα 13 R200K-YFP or Gα 13 Q226L-YFP expressing B16-F0 cells, upon RHOA inhibition treatment with CT04. Quantification of F-actin content ( f ) and circularity ( g ) in Gα 13 WT-YFP, Gα 13 R200K-YFP or Gα 13 Q226L-YFP expressing B16-F0 cells, upon ROCK inhibition treatment with Y27632. Means +/- SEM are shown.

    Techniques Used: Expressing, Generated, Inhibition



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    a,b Immunofluorescence images of Gα 13 WT-YFP, Gα 13 R200K-YFP or Gα 13 Q226L-YFP (green) expressed in NHEM. Left panels: The cells were labelled with phalloidin for visualising F-actin ( a ) or pMLC ( b ) (red), and Hoechst for nuclei (blue). Right panels: Quantifications of morphological parameters (perimeter, circularity and solidity) and F-actin content ( a ), and pMLC levels ( b ). c Quantification of <t>RHOA-GTP</t> levels in Gα 13 WT-YFP, Gα 13 R200K-YFP or Gα 13 Q226L-YFP -expressing NHEM cells. Means +/- SEM are shown.
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    Image Search Results


    a,b Immunofluorescence images of Gα 13 WT-YFP, Gα 13 R200K-YFP or Gα 13 Q226L-YFP (green) expressed in NHEM. Left panels: The cells were labelled with phalloidin for visualising F-actin ( a ) or pMLC ( b ) (red), and Hoechst for nuclei (blue). Right panels: Quantifications of morphological parameters (perimeter, circularity and solidity) and F-actin content ( a ), and pMLC levels ( b ). c Quantification of RHOA-GTP levels in Gα 13 WT-YFP, Gα 13 R200K-YFP or Gα 13 Q226L-YFP -expressing NHEM cells. Means +/- SEM are shown.

    Journal: medRxiv

    Article Title: A postzygotic GNA13 variant upregulates the RHOA/ROCK pathway and alters melanocyte function in a mosaic skin hypopigmentation syndrome

    doi: 10.1101/2024.07.24.24310661

    Figure Lengend Snippet: a,b Immunofluorescence images of Gα 13 WT-YFP, Gα 13 R200K-YFP or Gα 13 Q226L-YFP (green) expressed in NHEM. Left panels: The cells were labelled with phalloidin for visualising F-actin ( a ) or pMLC ( b ) (red), and Hoechst for nuclei (blue). Right panels: Quantifications of morphological parameters (perimeter, circularity and solidity) and F-actin content ( a ), and pMLC levels ( b ). c Quantification of RHOA-GTP levels in Gα 13 WT-YFP, Gα 13 R200K-YFP or Gα 13 Q226L-YFP -expressing NHEM cells. Means +/- SEM are shown.

    Article Snippet: Cells were incubated with primary antibodies anti-pMLC (Cell Signaling Technology), anti-vinculin (Life Technologies) or anti-RHOA-GTP (CurieCoreTech) for 1h at RT.

    Techniques: Immunofluorescence, Expressing

    a Quantification of RHOA-GTP levels in Gα 13 WT-YFP, Gα 13 R200K-YFP or Gα 13 Q226L-YFP expressing B16-F0 cells that correspond to the mean intensity of the signal generated from anti-GTP RHOA antibody labelling. Quantification of F-actin content ( b ) and circularity ( c ) in RHOA WT-GFP and RHOA Q63L-GFP expressing B16-F0 cells. Quantification of F-actin content ( d ) and circularity ( e ) in Gα 13 WT-YFP, Gα 13 R200K-YFP or Gα 13 Q226L-YFP expressing B16-F0 cells, upon RHOA inhibition treatment with CT04. Quantification of F-actin content ( f ) and circularity ( g ) in Gα 13 WT-YFP, Gα 13 R200K-YFP or Gα 13 Q226L-YFP expressing B16-F0 cells, upon ROCK inhibition treatment with Y27632. Means +/- SEM are shown.

    Journal: medRxiv

    Article Title: A postzygotic GNA13 variant upregulates the RHOA/ROCK pathway and alters melanocyte function in a mosaic skin hypopigmentation syndrome

    doi: 10.1101/2024.07.24.24310661

    Figure Lengend Snippet: a Quantification of RHOA-GTP levels in Gα 13 WT-YFP, Gα 13 R200K-YFP or Gα 13 Q226L-YFP expressing B16-F0 cells that correspond to the mean intensity of the signal generated from anti-GTP RHOA antibody labelling. Quantification of F-actin content ( b ) and circularity ( c ) in RHOA WT-GFP and RHOA Q63L-GFP expressing B16-F0 cells. Quantification of F-actin content ( d ) and circularity ( e ) in Gα 13 WT-YFP, Gα 13 R200K-YFP or Gα 13 Q226L-YFP expressing B16-F0 cells, upon RHOA inhibition treatment with CT04. Quantification of F-actin content ( f ) and circularity ( g ) in Gα 13 WT-YFP, Gα 13 R200K-YFP or Gα 13 Q226L-YFP expressing B16-F0 cells, upon ROCK inhibition treatment with Y27632. Means +/- SEM are shown.

    Article Snippet: Cells were incubated with primary antibodies anti-pMLC (Cell Signaling Technology), anti-vinculin (Life Technologies) or anti-RHOA-GTP (CurieCoreTech) for 1h at RT.

    Techniques: Expressing, Generated, Inhibition

    a,b Immunofluorescence images of Gα 13 WT-YFP, Gα 13 R200K-YFP or Gα 13 Q226L-YFP (green) expressed in NHEM. Left panels: The cells were labelled with phalloidin for visualising F-actin ( a ) or pMLC ( b ) (red), and Hoechst for nuclei (blue). Right panels: Quantifications of morphological parameters (perimeter, circularity and solidity) and F-actin content ( a ), and pMLC levels ( b ). c Quantification of RHOA-GTP levels in Gα 13 WT-YFP, Gα 13 R200K-YFP or Gα 13 Q226L-YFP -expressing NHEM cells. Means +/- SEM are shown.

    Journal: medRxiv

    Article Title: A postzygotic GNA13 variant upregulates the RHOA/ROCK pathway and alters melanocyte function in a mosaic skin hypopigmentation syndrome

    doi: 10.1101/2024.07.24.24310661

    Figure Lengend Snippet: a,b Immunofluorescence images of Gα 13 WT-YFP, Gα 13 R200K-YFP or Gα 13 Q226L-YFP (green) expressed in NHEM. Left panels: The cells were labelled with phalloidin for visualising F-actin ( a ) or pMLC ( b ) (red), and Hoechst for nuclei (blue). Right panels: Quantifications of morphological parameters (perimeter, circularity and solidity) and F-actin content ( a ), and pMLC levels ( b ). c Quantification of RHOA-GTP levels in Gα 13 WT-YFP, Gα 13 R200K-YFP or Gα 13 Q226L-YFP -expressing NHEM cells. Means +/- SEM are shown.

    Article Snippet: Cells were incubated with primary antibodies anti-pMLC (Cell Signaling Technology), anti-vinculin (Life Technologies) or anti-RHOA-GTP (CurieCoreTech) for 1h at RT.

    Techniques: Immunofluorescence, Expressing

    a Quantification of RHOA-GTP levels in Gα 13 WT-YFP, Gα 13 R200K-YFP or Gα 13 Q226L-YFP expressing B16-F0 cells that correspond to the mean intensity of the signal generated from anti-GTP RHOA antibody labelling. Quantification of F-actin content ( b ) and circularity ( c ) in RHOA WT-GFP and RHOA Q63L-GFP expressing B16-F0 cells. Quantification of F-actin content ( d ) and circularity ( e ) in Gα 13 WT-YFP, Gα 13 R200K-YFP or Gα 13 Q226L-YFP expressing B16-F0 cells, upon RHOA inhibition treatment with CT04. Quantification of F-actin content ( f ) and circularity ( g ) in Gα 13 WT-YFP, Gα 13 R200K-YFP or Gα 13 Q226L-YFP expressing B16-F0 cells, upon ROCK inhibition treatment with Y27632. Means +/- SEM are shown.

    Journal: medRxiv

    Article Title: A postzygotic GNA13 variant upregulates the RHOA/ROCK pathway and alters melanocyte function in a mosaic skin hypopigmentation syndrome

    doi: 10.1101/2024.07.24.24310661

    Figure Lengend Snippet: a Quantification of RHOA-GTP levels in Gα 13 WT-YFP, Gα 13 R200K-YFP or Gα 13 Q226L-YFP expressing B16-F0 cells that correspond to the mean intensity of the signal generated from anti-GTP RHOA antibody labelling. Quantification of F-actin content ( b ) and circularity ( c ) in RHOA WT-GFP and RHOA Q63L-GFP expressing B16-F0 cells. Quantification of F-actin content ( d ) and circularity ( e ) in Gα 13 WT-YFP, Gα 13 R200K-YFP or Gα 13 Q226L-YFP expressing B16-F0 cells, upon RHOA inhibition treatment with CT04. Quantification of F-actin content ( f ) and circularity ( g ) in Gα 13 WT-YFP, Gα 13 R200K-YFP or Gα 13 Q226L-YFP expressing B16-F0 cells, upon ROCK inhibition treatment with Y27632. Means +/- SEM are shown.

    Article Snippet: Cells were incubated with primary antibodies anti-pMLC (Cell Signaling Technology), anti-vinculin (Life Technologies) or anti-RHOA-GTP (CurieCoreTech) for 1h at RT.

    Techniques: Expressing, Generated, Inhibition