revertaid m mulv  (Thermo Fisher)


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    Name:
    RevertAid Reverse Transcriptase 200 U µL
    Description:
    Thermo Scientific RevertAid Reverse Transcriptase RT is a recombinant M MuLV RT It differs from the M MuLV RT by its structure and catalytic properties The enzyme possesses an RNA dependent and DNA dependent polymerase activity and a RNase H activity specific to RNA in RNA DNA hybrids which is significantly lower than that of Avian Myeloblastosis Virus AMV reverse transcriptase Highlights• Efficient synthesis of full length first strand cDNA up to 13 kb• Optimum activity at 42°C• Active up to 50°C• Incorporates modified nucleotides e g Cy3 Cy5 rhodamine aminoallyl fluorescein labeled nucleotides Applications• First strand cDNA synthesis for RT PCR and real time RT PCR• Synthesis of cDNA for cloning and expression• Generation of labeled cDNA probes for microarrays• DNA labeling• Analysis of RNA by primer extension
    Catalog Number:
    ep0441
    Price:
    None
    Applications:
    Cloning|PCR & Real-Time PCR|RT-PCR|Reverse Transcription|Two-Step RT-PCR|cDNA Libraries & Library Construction
    Category:
    Proteins Enzymes Peptides
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    Structured Review

    Thermo Fisher revertaid m mulv
    Thermo Scientific RevertAid Reverse Transcriptase RT is a recombinant M MuLV RT It differs from the M MuLV RT by its structure and catalytic properties The enzyme possesses an RNA dependent and DNA dependent polymerase activity and a RNase H activity specific to RNA in RNA DNA hybrids which is significantly lower than that of Avian Myeloblastosis Virus AMV reverse transcriptase Highlights• Efficient synthesis of full length first strand cDNA up to 13 kb• Optimum activity at 42°C• Active up to 50°C• Incorporates modified nucleotides e g Cy3 Cy5 rhodamine aminoallyl fluorescein labeled nucleotides Applications• First strand cDNA synthesis for RT PCR and real time RT PCR• Synthesis of cDNA for cloning and expression• Generation of labeled cDNA probes for microarrays• DNA labeling• Analysis of RNA by primer extension
    https://www.bioz.com/result/revertaid m mulv/product/Thermo Fisher
    Average 99 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    revertaid m mulv - by Bioz Stars, 2020-11
    99/100 stars

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    Synthesized:

    Article Title: Moss Pathogenesis-Related-10 Protein Enhances Resistance to Pythium irregulare in Physcomitrella patens and Arabidopsis thaliana
    Article Snippet: .. For cDNA synthesis, 2 μg of total RNA was treated with RNasefree DNase I (Thermo Scientific) to remove genomic DNA contamination. cDNA was synthesized from total RNA using RevertAid Reverse transcriptase (Thermo Scientific) and oligo (dT) according to the manufacturer’s protocol. .. From the resulting 25 μL of cDNA, 2 μL were used as a template for PCR analysis using PpPR10 specific primers (PpPR-10f and PpPR-10r).

    Article Title: Inhibition of Autophagy Amplifies Baicalein-Induced Apoptosis in Human Colorectal Cancer
    Article Snippet: .. The cDNA was synthesized from RNA using RevertAid Reverse Transcriptase (Thermo Fisher Scientific, USA). .. Thereafter, cDNA was amplified using the human apoptosis primer library (HPA-1; https://www.realtimeprimers.com/).

    Article Title: Long noncoding RNAs in vascular smooth muscle cells regulate vascular calcification
    Article Snippet: .. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) Complementary DNA (cDNA) was synthesized from total RNA using RevertAid Reverse Transcriptase (Thermo Fisher Scientific) and random hexamer primers (Thermo Fisher Scientific). qRT-PCR was conducted using Power SYBR Green PCR Master Mix (Thermo Fisher Scientific) in a Rotor-Gene Q (Qiagen). .. The primer sequences used for PCR are listed in Supplementary Table .

    Isolation:

    Article Title: Replacement of Murine Leukemia Virus Readthrough Mechanism by Human Immunodeficiency Virus Frameshift Allows Synthesis of Viral Proteins and Virus Replication
    Article Snippet: .. Briefly, the viral genomic RNA of chimeric or wild-type Mo-MuLV was isolated from resuspended viral particles and treated with fast-performance liquid chromatography pure DNase I (Amersham Pharmacia Biotech) before being used for the synthesis of a first DNA strand by using MuLV reverse transcriptase (Gibco-BRL) primed with an oligonucleotide located downstream of the Mfe I site (5′-gcc gta gga cag agg atg ag-3′). .. Amplification of double-stranded DNA was then achieved by using the same primer in combination with a second one located 5′ of the Xho I site (5′-gcc cca ttg gtc cca taa cc-3′).

    Quantitative RT-PCR:

    Article Title: Up-regulation of a cellular protein at the translational level by a retrovirus
    Article Snippet: .. RNA preparations were assessed for residual cellular DNA by standard PCRs using primers targeting the c-IAP1 gene. cDNA was prepared for qRT-PCR analysis by using the Mo-MLV reverse transcriptase enzyme (Invitrogen) and random hexamers as primers. .. SYBR-green-base qRT-PCR was used to assess relative c-IAP1 mRNA levels.

    Article Title: Long noncoding RNAs in vascular smooth muscle cells regulate vascular calcification
    Article Snippet: .. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) Complementary DNA (cDNA) was synthesized from total RNA using RevertAid Reverse Transcriptase (Thermo Fisher Scientific) and random hexamer primers (Thermo Fisher Scientific). qRT-PCR was conducted using Power SYBR Green PCR Master Mix (Thermo Fisher Scientific) in a Rotor-Gene Q (Qiagen). .. The primer sequences used for PCR are listed in Supplementary Table .

    SYBR Green Assay:

    Article Title: Long noncoding RNAs in vascular smooth muscle cells regulate vascular calcification
    Article Snippet: .. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) Complementary DNA (cDNA) was synthesized from total RNA using RevertAid Reverse Transcriptase (Thermo Fisher Scientific) and random hexamer primers (Thermo Fisher Scientific). qRT-PCR was conducted using Power SYBR Green PCR Master Mix (Thermo Fisher Scientific) in a Rotor-Gene Q (Qiagen). .. The primer sequences used for PCR are listed in Supplementary Table .

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Long noncoding RNAs in vascular smooth muscle cells regulate vascular calcification
    Article Snippet: .. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) Complementary DNA (cDNA) was synthesized from total RNA using RevertAid Reverse Transcriptase (Thermo Fisher Scientific) and random hexamer primers (Thermo Fisher Scientific). qRT-PCR was conducted using Power SYBR Green PCR Master Mix (Thermo Fisher Scientific) in a Rotor-Gene Q (Qiagen). .. The primer sequences used for PCR are listed in Supplementary Table .

    Random Hexamer Labeling:

    Article Title: Long noncoding RNAs in vascular smooth muscle cells regulate vascular calcification
    Article Snippet: .. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) Complementary DNA (cDNA) was synthesized from total RNA using RevertAid Reverse Transcriptase (Thermo Fisher Scientific) and random hexamer primers (Thermo Fisher Scientific). qRT-PCR was conducted using Power SYBR Green PCR Master Mix (Thermo Fisher Scientific) in a Rotor-Gene Q (Qiagen). .. The primer sequences used for PCR are listed in Supplementary Table .

    Polymerase Chain Reaction:

    Article Title: Long noncoding RNAs in vascular smooth muscle cells regulate vascular calcification
    Article Snippet: .. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) Complementary DNA (cDNA) was synthesized from total RNA using RevertAid Reverse Transcriptase (Thermo Fisher Scientific) and random hexamer primers (Thermo Fisher Scientific). qRT-PCR was conducted using Power SYBR Green PCR Master Mix (Thermo Fisher Scientific) in a Rotor-Gene Q (Qiagen). .. The primer sequences used for PCR are listed in Supplementary Table .

    Liquid Chromatography:

    Article Title: Replacement of Murine Leukemia Virus Readthrough Mechanism by Human Immunodeficiency Virus Frameshift Allows Synthesis of Viral Proteins and Virus Replication
    Article Snippet: .. Briefly, the viral genomic RNA of chimeric or wild-type Mo-MuLV was isolated from resuspended viral particles and treated with fast-performance liquid chromatography pure DNase I (Amersham Pharmacia Biotech) before being used for the synthesis of a first DNA strand by using MuLV reverse transcriptase (Gibco-BRL) primed with an oligonucleotide located downstream of the Mfe I site (5′-gcc gta gga cag agg atg ag-3′). .. Amplification of double-stranded DNA was then achieved by using the same primer in combination with a second one located 5′ of the Xho I site (5′-gcc cca ttg gtc cca taa cc-3′).

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  • 99
    Thermo Fisher m mlv reverse transcriptase 200 u µl
    Postentry steps of reovirus replication are strongly enhanced for T3D PL . (A) T3D PL , T3D KC , and T3D TD (MOIs of 1 to 3) were bound to L929 cell monolayers at 4°C and following washing to remove unbound virions, cells were incubated at 37°C and cell lysates were collected at various time points postinfection. Total proteins were separated using SDS-PAGE, and Western blot analysis with specific antibodies was used to identify reovirus proteins and β actin (loading control) (left). Densitometric band quantification of μ1C and δ and percent virus uncoating (right) were calculated using the following formula; [δ/(μ1C + δ)] ×100 ( n = 4 ± standard deviation; MOI of 1, n = 2; MOI of 3, n = 2). Linear regression analysis determined that the slopes are not significantly different. (B to D) L929 cells were exposed to T3D PL , T3D KC , and T3D TD (MOI of 3) at 4°C for 1 h, washed, and incubated at 37°C. At indicated time points, following total <t>RNA</t> extraction and <t>cDNA</t> synthesis, reovirus S4 and M2 RNA expression levels relative to the level of the housekeeping gene GAPDH were quantified using RT-qPCR ( n = 3 ± standard deviation; ***, P
    M Mlv Reverse Transcriptase 200 U µl, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m mlv reverse transcriptase 200 u µl/product/Thermo Fisher
    Average 99 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    m mlv reverse transcriptase 200 u µl - by Bioz Stars, 2020-11
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher m mulv reverse transcriptase
    Postentry steps of reovirus replication are strongly enhanced for T3D PL . (A) T3D PL , T3D KC , and T3D TD (MOIs of 1 to 3) were bound to L929 cell monolayers at 4°C and following washing to remove unbound virions, cells were incubated at 37°C and cell lysates were collected at various time points postinfection. Total proteins were separated using SDS-PAGE, and Western blot analysis with specific antibodies was used to identify reovirus proteins and β actin (loading control) (left). Densitometric band quantification of μ1C and δ and percent virus uncoating (right) were calculated using the following formula; [δ/(μ1C + δ)] ×100 ( n = 4 ± standard deviation; MOI of 1, n = 2; MOI of 3, n = 2). Linear regression analysis determined that the slopes are not significantly different. (B to D) L929 cells were exposed to T3D PL , T3D KC , and T3D TD (MOI of 3) at 4°C for 1 h, washed, and incubated at 37°C. At indicated time points, following total <t>RNA</t> extraction and <t>cDNA</t> synthesis, reovirus S4 and M2 RNA expression levels relative to the level of the housekeeping gene GAPDH were quantified using RT-qPCR ( n = 3 ± standard deviation; ***, P
    M Mulv Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 153 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m mulv reverse transcriptase/product/Thermo Fisher
    Average 99 stars, based on 153 article reviews
    Price from $9.99 to $1999.99
    m mulv reverse transcriptase - by Bioz Stars, 2020-11
    99/100 stars
      Buy from Supplier

    95
    Thermo Fisher m mlv reverse transcriptase
    Postentry steps of reovirus replication are strongly enhanced for T3D PL . (A) T3D PL , T3D KC , and T3D TD (MOIs of 1 to 3) were bound to L929 cell monolayers at 4°C and following washing to remove unbound virions, cells were incubated at 37°C and cell lysates were collected at various time points postinfection. Total proteins were separated using SDS-PAGE, and Western blot analysis with specific antibodies was used to identify reovirus proteins and β actin (loading control) (left). Densitometric band quantification of μ1C and δ and percent virus uncoating (right) were calculated using the following formula; [δ/(μ1C + δ)] ×100 ( n = 4 ± standard deviation; MOI of 1, n = 2; MOI of 3, n = 2). Linear regression analysis determined that the slopes are not significantly different. (B to D) L929 cells were exposed to T3D PL , T3D KC , and T3D TD (MOI of 3) at 4°C for 1 h, washed, and incubated at 37°C. At indicated time points, following total <t>RNA</t> extraction and <t>cDNA</t> synthesis, reovirus S4 and M2 RNA expression levels relative to the level of the housekeeping gene GAPDH were quantified using RT-qPCR ( n = 3 ± standard deviation; ***, P
    M Mlv Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 2749 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m mlv reverse transcriptase/product/Thermo Fisher
    Average 95 stars, based on 2749 article reviews
    Price from $9.99 to $1999.99
    m mlv reverse transcriptase - by Bioz Stars, 2020-11
    95/100 stars
      Buy from Supplier

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    Postentry steps of reovirus replication are strongly enhanced for T3D PL . (A) T3D PL , T3D KC , and T3D TD (MOIs of 1 to 3) were bound to L929 cell monolayers at 4°C and following washing to remove unbound virions, cells were incubated at 37°C and cell lysates were collected at various time points postinfection. Total proteins were separated using SDS-PAGE, and Western blot analysis with specific antibodies was used to identify reovirus proteins and β actin (loading control) (left). Densitometric band quantification of μ1C and δ and percent virus uncoating (right) were calculated using the following formula; [δ/(μ1C + δ)] ×100 ( n = 4 ± standard deviation; MOI of 1, n = 2; MOI of 3, n = 2). Linear regression analysis determined that the slopes are not significantly different. (B to D) L929 cells were exposed to T3D PL , T3D KC , and T3D TD (MOI of 3) at 4°C for 1 h, washed, and incubated at 37°C. At indicated time points, following total RNA extraction and cDNA synthesis, reovirus S4 and M2 RNA expression levels relative to the level of the housekeeping gene GAPDH were quantified using RT-qPCR ( n = 3 ± standard deviation; ***, P

    Journal: Journal of Virology

    Article Title: Polymorphisms in the Most Oncolytic Reovirus Strain Confer Enhanced Cell Attachment, Transcription, and Single-Step Replication Kinetics

    doi: 10.1128/JVI.01937-19

    Figure Lengend Snippet: Postentry steps of reovirus replication are strongly enhanced for T3D PL . (A) T3D PL , T3D KC , and T3D TD (MOIs of 1 to 3) were bound to L929 cell monolayers at 4°C and following washing to remove unbound virions, cells were incubated at 37°C and cell lysates were collected at various time points postinfection. Total proteins were separated using SDS-PAGE, and Western blot analysis with specific antibodies was used to identify reovirus proteins and β actin (loading control) (left). Densitometric band quantification of μ1C and δ and percent virus uncoating (right) were calculated using the following formula; [δ/(μ1C + δ)] ×100 ( n = 4 ± standard deviation; MOI of 1, n = 2; MOI of 3, n = 2). Linear regression analysis determined that the slopes are not significantly different. (B to D) L929 cells were exposed to T3D PL , T3D KC , and T3D TD (MOI of 3) at 4°C for 1 h, washed, and incubated at 37°C. At indicated time points, following total RNA extraction and cDNA synthesis, reovirus S4 and M2 RNA expression levels relative to the level of the housekeeping gene GAPDH were quantified using RT-qPCR ( n = 3 ± standard deviation; ***, P

    Article Snippet: Using 10 μg of glycogen (R0551; ThermoFisher Scientific) as a carrier according to manufacturer’s instructions, RNA was purified and converted to cDNA (28025013; ThermoFisher Scientific) using random primers (48190011; ThermoFisher Scientific), and RT-PCR (1725204; Bio-Rad) was performed to quantify reovirus S4, reovirus M2, and mouse GAPDH.

    Techniques: Incubation, SDS Page, Western Blot, Standard Deviation, RNA Extraction, RNA Expression, Quantitative RT-PCR