revertaid h minus m mulv reverse transcriptase  (Thermo Fisher)


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    RevertAid H Minus Reverse Transcriptase 200 U µL
    Description:
    Thermo Scientific RevertAid H Minus Reverse Transcriptase is a recombinant M MuLV RT The enzyme possesses an RNA dependent and DNA dependent polymerase activity but lacks RNase H activity due to a point mutation in the RNase H domain RevertAid H Minus Reverse Transcriptase does not degrade RNA in RNA DNA hybrids during synthesis of the first strand cDNA and therefore high yields of full length cDNA from long templates are obtained RevertAid H Minus Reverse Transcriptase maintains activity over a wide temperature range 42 to 50°C Highlights• High yields of full length first strand cDNA up to 13 kb• Optimum activity at 42 to 45°C• Active up to 55°C• Incorporates modified nucleotides e g Cy3 Cy5 rhodamine aminoallyl fluorescein labeled nucleotides Applications• First strand cDNA synthesis for RT PCR and real time RT PCR• Reverse transcription at elevated temperatures to reduce effects of secondary structure• Synthesis of cDNA for cloning and expression• Generation of labeled cDNA probes for microarrays• DNA labeling• Analysis of RNA by primer extensionRelated ProductsRevertAid H Minus Reverse Transcriptase 200 U µL
    Catalog Number:
    EP0451
    Price:
    None
    Category:
    Proteins Enzymes Peptides
    Applications:
    Cloning|PCR & Real-Time PCR|RT-PCR|Reverse Transcription|Two-Step RT-PCR|cDNA Libraries & Library Construction
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    Structured Review

    Thermo Fisher revertaid h minus m mulv reverse transcriptase
    Thermo Scientific RevertAid H Minus Reverse Transcriptase is a recombinant M MuLV RT The enzyme possesses an RNA dependent and DNA dependent polymerase activity but lacks RNase H activity due to a point mutation in the RNase H domain RevertAid H Minus Reverse Transcriptase does not degrade RNA in RNA DNA hybrids during synthesis of the first strand cDNA and therefore high yields of full length cDNA from long templates are obtained RevertAid H Minus Reverse Transcriptase maintains activity over a wide temperature range 42 to 50°C Highlights• High yields of full length first strand cDNA up to 13 kb• Optimum activity at 42 to 45°C• Active up to 55°C• Incorporates modified nucleotides e g Cy3 Cy5 rhodamine aminoallyl fluorescein labeled nucleotides Applications• First strand cDNA synthesis for RT PCR and real time RT PCR• Reverse transcription at elevated temperatures to reduce effects of secondary structure• Synthesis of cDNA for cloning and expression• Generation of labeled cDNA probes for microarrays• DNA labeling• Analysis of RNA by primer extensionRelated ProductsRevertAid H Minus Reverse Transcriptase 200 U µL
    https://www.bioz.com/result/revertaid h minus m mulv reverse transcriptase/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    revertaid h minus m mulv reverse transcriptase - by Bioz Stars, 2021-05
    99/100 stars

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    Related Articles

    Infection:

    Article Title: The Enzymatic Activity of the nsp14 Exoribonuclease Is Critical for Replication of MERS-CoV and SARS-CoV-2
    Article Snippet: Virus titers were determined by plaque assay in HuH7 and Vero cells ( ). .. In order to confirm the presence of engineered mutations in viral progeny, HuH7 and Vero cells were infected with supernatants harvested from transfected cells and intracellular RNA was isolated at 18 h postinfection as described above. cDNA was synthesized by reverse transcription using RevertAid H minus reverse transcriptase (Thermo Fisher Scientific) and random hexamer primers (Promega), in combination with a primer targeting the 3′ end of the viral genome. .. The full-length genome or the nsp14-coding region was amplified by PCR using MyTaq DNA polymerase (Bioline), and after purification, the PCR product was sequenced by Sanger sequencing.

    Transfection:

    Article Title: The Enzymatic Activity of the nsp14 Exoribonuclease Is Critical for Replication of MERS-CoV and SARS-CoV-2
    Article Snippet: Virus titers were determined by plaque assay in HuH7 and Vero cells ( ). .. In order to confirm the presence of engineered mutations in viral progeny, HuH7 and Vero cells were infected with supernatants harvested from transfected cells and intracellular RNA was isolated at 18 h postinfection as described above. cDNA was synthesized by reverse transcription using RevertAid H minus reverse transcriptase (Thermo Fisher Scientific) and random hexamer primers (Promega), in combination with a primer targeting the 3′ end of the viral genome. .. The full-length genome or the nsp14-coding region was amplified by PCR using MyTaq DNA polymerase (Bioline), and after purification, the PCR product was sequenced by Sanger sequencing.

    Isolation:

    Article Title: The Enzymatic Activity of the nsp14 Exoribonuclease Is Critical for Replication of MERS-CoV and SARS-CoV-2
    Article Snippet: Virus titers were determined by plaque assay in HuH7 and Vero cells ( ). .. In order to confirm the presence of engineered mutations in viral progeny, HuH7 and Vero cells were infected with supernatants harvested from transfected cells and intracellular RNA was isolated at 18 h postinfection as described above. cDNA was synthesized by reverse transcription using RevertAid H minus reverse transcriptase (Thermo Fisher Scientific) and random hexamer primers (Promega), in combination with a primer targeting the 3′ end of the viral genome. .. The full-length genome or the nsp14-coding region was amplified by PCR using MyTaq DNA polymerase (Bioline), and after purification, the PCR product was sequenced by Sanger sequencing.

    Synthesized:

    Article Title: The Enzymatic Activity of the nsp14 Exoribonuclease Is Critical for Replication of MERS-CoV and SARS-CoV-2
    Article Snippet: Virus titers were determined by plaque assay in HuH7 and Vero cells ( ). .. In order to confirm the presence of engineered mutations in viral progeny, HuH7 and Vero cells were infected with supernatants harvested from transfected cells and intracellular RNA was isolated at 18 h postinfection as described above. cDNA was synthesized by reverse transcription using RevertAid H minus reverse transcriptase (Thermo Fisher Scientific) and random hexamer primers (Promega), in combination with a primer targeting the 3′ end of the viral genome. .. The full-length genome or the nsp14-coding region was amplified by PCR using MyTaq DNA polymerase (Bioline), and after purification, the PCR product was sequenced by Sanger sequencing.

    Random Hexamer Labeling:

    Article Title: The Enzymatic Activity of the nsp14 Exoribonuclease Is Critical for Replication of MERS-CoV and SARS-CoV-2
    Article Snippet: Virus titers were determined by plaque assay in HuH7 and Vero cells ( ). .. In order to confirm the presence of engineered mutations in viral progeny, HuH7 and Vero cells were infected with supernatants harvested from transfected cells and intracellular RNA was isolated at 18 h postinfection as described above. cDNA was synthesized by reverse transcription using RevertAid H minus reverse transcriptase (Thermo Fisher Scientific) and random hexamer primers (Promega), in combination with a primer targeting the 3′ end of the viral genome. .. The full-length genome or the nsp14-coding region was amplified by PCR using MyTaq DNA polymerase (Bioline), and after purification, the PCR product was sequenced by Sanger sequencing.

    Mouse Assay:

    Article Title: Wild-type and innate immune-deficient mice are not susceptible to the Middle East respiratory syndrome coronavirus
    Article Snippet: .. RNA was extracted from lungs of WT BALB/c, 129S6/SvEv and 129/STAT1−/− mice at the time points noted above and converted into randomly primed cDNA using Revertaid Reverse Transcriptase (Thermo Scientific) according to the manufacturer’s instructions. .. When cDNA samples were probed for MERS-E ( ) or MERS-ORF1B ( ) transcripts, which probe for only viral genomic RNA levels, there were significantly higher levels compared to mock infected controls.

    Labeling:

    Article Title: Introns provide a platform for intergenic regulatory feedback of RPL22 paralogs in yeast
    Article Snippet: Briefly, RNA was isolated using the MasterPure Yeast RNA Purification Kit (Epicentre Biotechnologies). .. Three to five micrograms of RNA were transcribed into cDNA using RevertAid reverse transcriptase (Thermo Fisher Scientific) with the YAC06 and YU14 [ ] primers labeled using γ32 P-ATP and the T4 polynucleotide kinase (Promega). cDNA molecules were then separated on 8% polyacrylamide gel with 7 M urea together with a ΦX174 HinfI DNA labeled marker. .. Radioactive signals were captured on Imaging Screen-K (BioRad) and detected using the Typhoon FLA imager (GE Healthcare Life Sciences).

    Marker:

    Article Title: Introns provide a platform for intergenic regulatory feedback of RPL22 paralogs in yeast
    Article Snippet: Briefly, RNA was isolated using the MasterPure Yeast RNA Purification Kit (Epicentre Biotechnologies). .. Three to five micrograms of RNA were transcribed into cDNA using RevertAid reverse transcriptase (Thermo Fisher Scientific) with the YAC06 and YU14 [ ] primers labeled using γ32 P-ATP and the T4 polynucleotide kinase (Promega). cDNA molecules were then separated on 8% polyacrylamide gel with 7 M urea together with a ΦX174 HinfI DNA labeled marker. .. Radioactive signals were captured on Imaging Screen-K (BioRad) and detected using the Typhoon FLA imager (GE Healthcare Life Sciences).

    Polymerase Chain Reaction:

    Article Title: Klebsiella pneumoniae yggG Gene Product: A Zinc-Dependent Metalloprotease
    Article Snippet: The purified protein was eluted as un-tagged YggG after overnight thrombin cleavage and quantified by using Bradford reagent (Bio-Rad). .. Reverse Transcription PCR of yggG Gene RevertAid H Minus first strand cDNA synthesis kit (Fermentas) was used to synthesize the cDNA from the extracted total RNA. .. One microgram of total RNA was mixed with 0.2 μg random hexamer primer and DEPC-treated water.

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  • 99
    Thermo Fisher revertaid h minus m mulv reverse transcriptase
    Revertaid H Minus M Mulv Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/revertaid h minus m mulv reverse transcriptase/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    revertaid h minus m mulv reverse transcriptase - by Bioz Stars, 2021-05
    99/100 stars
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