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tiangen biotech co reverse transcription quantitative pcr rt qpcr
Schematic diagram of plasmids for expressing the var aslncRNA and 5′ end identification of PF3D7_0617400 aslncRNA. (A) Schematic diagram of pT7SE-as0617400 and pT7-as0617400. The translation initial site of ATG of PF3D7_0617400 is marked as +1 and the stop codon is marked with +8375, the ∼2.5 kb var fragment (from +6412 to +3952) was amplified and inserted into pT7SE and pT7, respectively. The wavy line is the natural var aslncRNA. T7 pro, T7 promoter; T7 ter, T7 terminator. (B) 5′ end identification of PF3D7_0617400 aslncRNA. <t>PCR</t> products of 5′ end RACE obtained according to the standard manuals. M: the DNA marker λ-EcoT14 I digest (TAKARA), lane1: PCR products of 5′ RACE. (C) The PF3D7_0617400 aslncRNA transcriptional start sites. The translation start site of PF3D7_0617400 is marked as +1 on the schematic diagram. Capital letters are the coding sequence of the var exonI and the lower letters are intron sequence. The transcriptional start sites of the aslncRNAs are marked with underlined numbers and arrows. The underlined letters locations are displayed relative to translation start site, and the arrows indicate the aslncRNA transcription directions. (D) <t>RT-qPCR</t> quantification of the NLS-T7RNP transcription in C8/pT7- as0617400 ( 1 ) and C8/pT7SE-as0617400 ( 2 ). Relative transcripts numbers are normalized to serine-tRNA ligase gene ( PF3D7_0717700 ). ∗ P
Reverse Transcription Quantitative Pcr Rt Qpcr, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Plasmodium falciparumvar Gene Is Activated by Its Antisense Long Noncoding RNA"

Article Title: Plasmodium falciparumvar Gene Is Activated by Its Antisense Long Noncoding RNA

Journal: Frontiers in Microbiology

doi: 10.3389/fmicb.2018.03117

Schematic diagram of plasmids for expressing the var aslncRNA and 5′ end identification of PF3D7_0617400 aslncRNA. (A) Schematic diagram of pT7SE-as0617400 and pT7-as0617400. The translation initial site of ATG of PF3D7_0617400 is marked as +1 and the stop codon is marked with +8375, the ∼2.5 kb var fragment (from +6412 to +3952) was amplified and inserted into pT7SE and pT7, respectively. The wavy line is the natural var aslncRNA. T7 pro, T7 promoter; T7 ter, T7 terminator. (B) 5′ end identification of PF3D7_0617400 aslncRNA. PCR products of 5′ end RACE obtained according to the standard manuals. M: the DNA marker λ-EcoT14 I digest (TAKARA), lane1: PCR products of 5′ RACE. (C) The PF3D7_0617400 aslncRNA transcriptional start sites. The translation start site of PF3D7_0617400 is marked as +1 on the schematic diagram. Capital letters are the coding sequence of the var exonI and the lower letters are intron sequence. The transcriptional start sites of the aslncRNAs are marked with underlined numbers and arrows. The underlined letters locations are displayed relative to translation start site, and the arrows indicate the aslncRNA transcription directions. (D) RT-qPCR quantification of the NLS-T7RNP transcription in C8/pT7- as0617400 ( 1 ) and C8/pT7SE-as0617400 ( 2 ). Relative transcripts numbers are normalized to serine-tRNA ligase gene ( PF3D7_0717700 ). ∗ P
Figure Legend Snippet: Schematic diagram of plasmids for expressing the var aslncRNA and 5′ end identification of PF3D7_0617400 aslncRNA. (A) Schematic diagram of pT7SE-as0617400 and pT7-as0617400. The translation initial site of ATG of PF3D7_0617400 is marked as +1 and the stop codon is marked with +8375, the ∼2.5 kb var fragment (from +6412 to +3952) was amplified and inserted into pT7SE and pT7, respectively. The wavy line is the natural var aslncRNA. T7 pro, T7 promoter; T7 ter, T7 terminator. (B) 5′ end identification of PF3D7_0617400 aslncRNA. PCR products of 5′ end RACE obtained according to the standard manuals. M: the DNA marker λ-EcoT14 I digest (TAKARA), lane1: PCR products of 5′ RACE. (C) The PF3D7_0617400 aslncRNA transcriptional start sites. The translation start site of PF3D7_0617400 is marked as +1 on the schematic diagram. Capital letters are the coding sequence of the var exonI and the lower letters are intron sequence. The transcriptional start sites of the aslncRNAs are marked with underlined numbers and arrows. The underlined letters locations are displayed relative to translation start site, and the arrows indicate the aslncRNA transcription directions. (D) RT-qPCR quantification of the NLS-T7RNP transcription in C8/pT7- as0617400 ( 1 ) and C8/pT7SE-as0617400 ( 2 ). Relative transcripts numbers are normalized to serine-tRNA ligase gene ( PF3D7_0717700 ). ∗ P

Techniques Used: Expressing, Amplification, Polymerase Chain Reaction, Marker, Sequencing, Quantitative RT-PCR

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Polymerase Chain Reaction:

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Article Snippet: .. Reverse transcription-quantitative PCR (RT-qPCR) was conducted with SYBR green (SuperReal-Premix Plus; Tiangen, China). .. All of these reactions were run on an ABI7500 real-time PCR system (Applied Biosystems, USA), and the agents for these assays were used in accordance with the manufacturer’s instructions.

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SYBR Green Assay:

Article Title: Two Verticillium dahliae MAPKKKs, VdSsk2 and VdSte11, Have Distinct Roles in Pathogenicity, Microsclerotial Formation, and Stress Adaptation
Article Snippet: .. Reverse transcription-quantitative PCR (RT-qPCR) was conducted with SYBR green (SuperReal-Premix Plus; Tiangen, China). .. All of these reactions were run on an ABI7500 real-time PCR system (Applied Biosystems, USA), and the agents for these assays were used in accordance with the manufacturer’s instructions.

Real-time Polymerase Chain Reaction:

Article Title: microRNA-548b suppresses aggressive phenotypes of hepatocellular carcinoma by directly targeting high-mobility group box 1 mRNA
Article Snippet: .. RNA isolation and reverse-transcription quantitative PCR (RT-qPCR) The miRcute Extraction and Separation of miRNAs Kit (DP501; Tiangen, Beijing, China) was used to extract miRNAs from cells and homogenized tissues. .. For evaluation of the miR-548b expression, cDNA synthesis was carried out with the miScript Reverse Transcription Kit (Qiagen GmbH, Hilden, Germany).

Article Title: Plasmodium falciparumvar Gene Is Activated by Its Antisense Long Noncoding RNA
Article Snippet: .. Reverse Transcription Quantitative PCR (RT-qPCR) One microgram of the extracted total RNA of P. falciparum was reverse transcribed using FastQuant RT Kit (Tiangen) according to its standard manuals. .. The RT-qPCR was performed as described , and serine-tRNA ligase (PF3D7_0717700 ) was used as an internal control.

Article Title: PTEN/AKT/mTOR signaling mediates anticancer effects of epigallocatechin-3-gallate in ovarian cancer
Article Snippet: .. Reverse transcription-quantitative PCR (RT-qPCR) Following EGCG treatment for 48 h at 37°C, total RNA was extracted from SKOV3 cells using TRIzol® reagent (Tiangen Biotech Co., Ltd.) and reverse-transcribed into cDNA using a GoScript™ Reverse Transcription Mix (Promega, Biotech Co., Ltd, Beijing) at 42°C for 20 min and 90°C for 5 min. QPCR analysis was performed using UltraSYBR Mixture (CW Bio) on the ABI 7500 Fast Real-Time PCR Detection system (Thermo Fisher Scientific, Inc.). ..

Quantitative RT-PCR:

Article Title: Two Verticillium dahliae MAPKKKs, VdSsk2 and VdSte11, Have Distinct Roles in Pathogenicity, Microsclerotial Formation, and Stress Adaptation
Article Snippet: .. Reverse transcription-quantitative PCR (RT-qPCR) was conducted with SYBR green (SuperReal-Premix Plus; Tiangen, China). .. All of these reactions were run on an ABI7500 real-time PCR system (Applied Biosystems, USA), and the agents for these assays were used in accordance with the manufacturer’s instructions.

Article Title: microRNA-548b suppresses aggressive phenotypes of hepatocellular carcinoma by directly targeting high-mobility group box 1 mRNA
Article Snippet: .. RNA isolation and reverse-transcription quantitative PCR (RT-qPCR) The miRcute Extraction and Separation of miRNAs Kit (DP501; Tiangen, Beijing, China) was used to extract miRNAs from cells and homogenized tissues. .. For evaluation of the miR-548b expression, cDNA synthesis was carried out with the miScript Reverse Transcription Kit (Qiagen GmbH, Hilden, Germany).

Article Title: Plasmodium falciparumvar Gene Is Activated by Its Antisense Long Noncoding RNA
Article Snippet: .. Reverse Transcription Quantitative PCR (RT-qPCR) One microgram of the extracted total RNA of P. falciparum was reverse transcribed using FastQuant RT Kit (Tiangen) according to its standard manuals. .. The RT-qPCR was performed as described , and serine-tRNA ligase (PF3D7_0717700 ) was used as an internal control.

Article Title: PTEN/AKT/mTOR signaling mediates anticancer effects of epigallocatechin-3-gallate in ovarian cancer
Article Snippet: .. Reverse transcription-quantitative PCR (RT-qPCR) Following EGCG treatment for 48 h at 37°C, total RNA was extracted from SKOV3 cells using TRIzol® reagent (Tiangen Biotech Co., Ltd.) and reverse-transcribed into cDNA using a GoScript™ Reverse Transcription Mix (Promega, Biotech Co., Ltd, Beijing) at 42°C for 20 min and 90°C for 5 min. QPCR analysis was performed using UltraSYBR Mixture (CW Bio) on the ABI 7500 Fast Real-Time PCR Detection system (Thermo Fisher Scientific, Inc.). ..

Article Title: A novel miRNA negatively regulates resistance to Glomerella leaf spot by suppressing expression of an NBS gene in apple
Article Snippet: .. Reverse transcription-quantitative PCR (RT-qPCR) was performed using miRcute plus miRNA Premix and SuperReal PreMix Plus (Tiangen Biotech Co., Ltd., China). .. Thermocycling involved a program of 40 cycles of 95 °C for 10 s and 60 °C for 30 s with a StepOnePlus Real-Time PCR System (Thermo Fisher Scientific, USA).

Isolation:

Article Title: microRNA-548b suppresses aggressive phenotypes of hepatocellular carcinoma by directly targeting high-mobility group box 1 mRNA
Article Snippet: .. RNA isolation and reverse-transcription quantitative PCR (RT-qPCR) The miRcute Extraction and Separation of miRNAs Kit (DP501; Tiangen, Beijing, China) was used to extract miRNAs from cells and homogenized tissues. .. For evaluation of the miR-548b expression, cDNA synthesis was carried out with the miScript Reverse Transcription Kit (Qiagen GmbH, Hilden, Germany).

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    tiangen biotech co reverse transcription quantitative pcr rt qpcr
    Schematic diagram of plasmids for expressing the var aslncRNA and 5′ end identification of PF3D7_0617400 aslncRNA. (A) Schematic diagram of pT7SE-as0617400 and pT7-as0617400. The translation initial site of ATG of PF3D7_0617400 is marked as +1 and the stop codon is marked with +8375, the ∼2.5 kb var fragment (from +6412 to +3952) was amplified and inserted into pT7SE and pT7, respectively. The wavy line is the natural var aslncRNA. T7 pro, T7 promoter; T7 ter, T7 terminator. (B) 5′ end identification of PF3D7_0617400 aslncRNA. <t>PCR</t> products of 5′ end RACE obtained according to the standard manuals. M: the DNA marker λ-EcoT14 I digest (TAKARA), lane1: PCR products of 5′ RACE. (C) The PF3D7_0617400 aslncRNA transcriptional start sites. The translation start site of PF3D7_0617400 is marked as +1 on the schematic diagram. Capital letters are the coding sequence of the var exonI and the lower letters are intron sequence. The transcriptional start sites of the aslncRNAs are marked with underlined numbers and arrows. The underlined letters locations are displayed relative to translation start site, and the arrows indicate the aslncRNA transcription directions. (D) <t>RT-qPCR</t> quantification of the NLS-T7RNP transcription in C8/pT7- as0617400 ( 1 ) and C8/pT7SE-as0617400 ( 2 ). Relative transcripts numbers are normalized to serine-tRNA ligase gene ( PF3D7_0717700 ). ∗ P
    Reverse Transcription Quantitative Pcr Rt Qpcr, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/reverse transcription quantitative pcr rt qpcr/product/tiangen biotech co
    Average 92 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    reverse transcription quantitative pcr rt qpcr - by Bioz Stars, 2020-07
    92/100 stars
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    Schematic diagram of plasmids for expressing the var aslncRNA and 5′ end identification of PF3D7_0617400 aslncRNA. (A) Schematic diagram of pT7SE-as0617400 and pT7-as0617400. The translation initial site of ATG of PF3D7_0617400 is marked as +1 and the stop codon is marked with +8375, the ∼2.5 kb var fragment (from +6412 to +3952) was amplified and inserted into pT7SE and pT7, respectively. The wavy line is the natural var aslncRNA. T7 pro, T7 promoter; T7 ter, T7 terminator. (B) 5′ end identification of PF3D7_0617400 aslncRNA. PCR products of 5′ end RACE obtained according to the standard manuals. M: the DNA marker λ-EcoT14 I digest (TAKARA), lane1: PCR products of 5′ RACE. (C) The PF3D7_0617400 aslncRNA transcriptional start sites. The translation start site of PF3D7_0617400 is marked as +1 on the schematic diagram. Capital letters are the coding sequence of the var exonI and the lower letters are intron sequence. The transcriptional start sites of the aslncRNAs are marked with underlined numbers and arrows. The underlined letters locations are displayed relative to translation start site, and the arrows indicate the aslncRNA transcription directions. (D) RT-qPCR quantification of the NLS-T7RNP transcription in C8/pT7- as0617400 ( 1 ) and C8/pT7SE-as0617400 ( 2 ). Relative transcripts numbers are normalized to serine-tRNA ligase gene ( PF3D7_0717700 ). ∗ P

    Journal: Frontiers in Microbiology

    Article Title: Plasmodium falciparumvar Gene Is Activated by Its Antisense Long Noncoding RNA

    doi: 10.3389/fmicb.2018.03117

    Figure Lengend Snippet: Schematic diagram of plasmids for expressing the var aslncRNA and 5′ end identification of PF3D7_0617400 aslncRNA. (A) Schematic diagram of pT7SE-as0617400 and pT7-as0617400. The translation initial site of ATG of PF3D7_0617400 is marked as +1 and the stop codon is marked with +8375, the ∼2.5 kb var fragment (from +6412 to +3952) was amplified and inserted into pT7SE and pT7, respectively. The wavy line is the natural var aslncRNA. T7 pro, T7 promoter; T7 ter, T7 terminator. (B) 5′ end identification of PF3D7_0617400 aslncRNA. PCR products of 5′ end RACE obtained according to the standard manuals. M: the DNA marker λ-EcoT14 I digest (TAKARA), lane1: PCR products of 5′ RACE. (C) The PF3D7_0617400 aslncRNA transcriptional start sites. The translation start site of PF3D7_0617400 is marked as +1 on the schematic diagram. Capital letters are the coding sequence of the var exonI and the lower letters are intron sequence. The transcriptional start sites of the aslncRNAs are marked with underlined numbers and arrows. The underlined letters locations are displayed relative to translation start site, and the arrows indicate the aslncRNA transcription directions. (D) RT-qPCR quantification of the NLS-T7RNP transcription in C8/pT7- as0617400 ( 1 ) and C8/pT7SE-as0617400 ( 2 ). Relative transcripts numbers are normalized to serine-tRNA ligase gene ( PF3D7_0717700 ). ∗ P

    Article Snippet: Reverse Transcription Quantitative PCR (RT-qPCR) One microgram of the extracted total RNA of P. falciparum was reverse transcribed using FastQuant RT Kit (Tiangen) according to its standard manuals.

    Techniques: Expressing, Amplification, Polymerase Chain Reaction, Marker, Sequencing, Quantitative RT-PCR