reverse transcription quantitative pcr rt qpcr  (Toyobo)

 
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    Toyobo reverse transcription quantitative pcr rt qpcr
    Results of <t>RT-qPCR</t> for protein coding genes. Gene specific primers for RT-qPCR were mentioned in Table S2 . The detailes of <t>PCR</t> condition for each target gene were descrived in Supporting Information S1 . (A)–(D): Bar graphs showing gene expression relative to gapdh (means; bars = S.D). MH: adult male head (n = 3), MA: adult male abdomen (n = 3), FH: adult female head (n = 3), FA; adult female abdomen (n = 3), cleavage: cleavage-stage embryo (1–8 h post-oviposition, n = 9), morula: primary morula-stage embryo (12–24 h post-oviposition, n = 12). No differences were determined to be statistically significant for any comparison of pairs at p = 0.05 (two-sample t-test or Welch's test).
    Reverse Transcription Quantitative Pcr Rt Qpcr, supplied by Toyobo, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/reverse transcription quantitative pcr rt qpcr/product/Toyobo
    Average 92 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    reverse transcription quantitative pcr rt qpcr - by Bioz Stars, 2020-07
    92/100 stars

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    1) Product Images from "Gene Expression of Protein-Coding and Non-Coding RNAs Related to Polyembryogenesis in the Parasitic Wasp, Copidosoma floridanum"

    Article Title: Gene Expression of Protein-Coding and Non-Coding RNAs Related to Polyembryogenesis in the Parasitic Wasp, Copidosoma floridanum

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0114372

    Results of RT-qPCR for protein coding genes. Gene specific primers for RT-qPCR were mentioned in Table S2 . The detailes of PCR condition for each target gene were descrived in Supporting Information S1 . (A)–(D): Bar graphs showing gene expression relative to gapdh (means; bars = S.D). MH: adult male head (n = 3), MA: adult male abdomen (n = 3), FH: adult female head (n = 3), FA; adult female abdomen (n = 3), cleavage: cleavage-stage embryo (1–8 h post-oviposition, n = 9), morula: primary morula-stage embryo (12–24 h post-oviposition, n = 12). No differences were determined to be statistically significant for any comparison of pairs at p = 0.05 (two-sample t-test or Welch's test).
    Figure Legend Snippet: Results of RT-qPCR for protein coding genes. Gene specific primers for RT-qPCR were mentioned in Table S2 . The detailes of PCR condition for each target gene were descrived in Supporting Information S1 . (A)–(D): Bar graphs showing gene expression relative to gapdh (means; bars = S.D). MH: adult male head (n = 3), MA: adult male abdomen (n = 3), FH: adult female head (n = 3), FA; adult female abdomen (n = 3), cleavage: cleavage-stage embryo (1–8 h post-oviposition, n = 9), morula: primary morula-stage embryo (12–24 h post-oviposition, n = 12). No differences were determined to be statistically significant for any comparison of pairs at p = 0.05 (two-sample t-test or Welch's test).

    Techniques Used: Quantitative RT-PCR, Polymerase Chain Reaction, Expressing

    Results of RT-qPCR for CflncRNAs. Gene specific primers for RT-qPCR were mentioned in Table S2 . The detailes of PCR condition for each target gene were descrived in Supporting Information S1 . (A)–(C): Bar graphs showing the gene expression relative to gapdh (means, bars = S.D). MH: adult male head (n = 3), MA: adult male abdomen (n = 3), FH: adult female head (n = 3), FA; adult female abdomen (n = 3), cleavage: cleavage-stage embryo (1–8 h post-oviposition, n = 9), morula: primary morula-stage embryo (12–24 h post-oviposition, n = 12). For A and B, *indicates a statistically significant difference, p
    Figure Legend Snippet: Results of RT-qPCR for CflncRNAs. Gene specific primers for RT-qPCR were mentioned in Table S2 . The detailes of PCR condition for each target gene were descrived in Supporting Information S1 . (A)–(C): Bar graphs showing the gene expression relative to gapdh (means, bars = S.D). MH: adult male head (n = 3), MA: adult male abdomen (n = 3), FH: adult female head (n = 3), FA; adult female abdomen (n = 3), cleavage: cleavage-stage embryo (1–8 h post-oviposition, n = 9), morula: primary morula-stage embryo (12–24 h post-oviposition, n = 12). For A and B, *indicates a statistically significant difference, p

    Techniques Used: Quantitative RT-PCR, Polymerase Chain Reaction, Expressing

    Related Articles

    Polymerase Chain Reaction:

    Article Title: RNA-Seq Analysis of the Response of the Halophyte, Mesembryanthemum crystallinum (Ice Plant) to High Salinity
    Article Snippet: .. Reverse transcription—quantitative PCR (RT-qPCR) was performed using THUNDERBIRD SYBR qPCR Mix (TOYOBO) on an ABI 7500 Real-Time PCR (Applied Biosystems). .. RT-qPCR reactions were performed in a total volume of 25 μl; with 1 μl of first-strand cDNAs and 1 μl of each primer.

    Real-time Polymerase Chain Reaction:

    Article Title: RNA-Seq Analysis of the Response of the Halophyte, Mesembryanthemum crystallinum (Ice Plant) to High Salinity
    Article Snippet: .. Reverse transcription—quantitative PCR (RT-qPCR) was performed using THUNDERBIRD SYBR qPCR Mix (TOYOBO) on an ABI 7500 Real-Time PCR (Applied Biosystems). .. RT-qPCR reactions were performed in a total volume of 25 μl; with 1 μl of first-strand cDNAs and 1 μl of each primer.

    Article Title: Gene Expression of Protein-Coding and Non-Coding RNAs Related to Polyembryogenesis in the Parasitic Wasp, Copidosoma floridanum
    Article Snippet: .. Reverse transcription quantitative PCR (RT-qPCR) was performed using the same cDNA samples as templates with THUNDERBIRD SYBR qPCR Mix (TOYOBO) and gene-specific primers ( ). .. All runs were carried out using MiniOpticon (BIO-RAD) system and the data were analyzed using CFX Manager 3.1 (BIO-RAD) software.

    Quantitative RT-PCR:

    Article Title: RNA-Seq Analysis of the Response of the Halophyte, Mesembryanthemum crystallinum (Ice Plant) to High Salinity
    Article Snippet: .. Reverse transcription—quantitative PCR (RT-qPCR) was performed using THUNDERBIRD SYBR qPCR Mix (TOYOBO) on an ABI 7500 Real-Time PCR (Applied Biosystems). .. RT-qPCR reactions were performed in a total volume of 25 μl; with 1 μl of first-strand cDNAs and 1 μl of each primer.

    Article Title: Gene Expression of Protein-Coding and Non-Coding RNAs Related to Polyembryogenesis in the Parasitic Wasp, Copidosoma floridanum
    Article Snippet: .. Reverse transcription quantitative PCR (RT-qPCR) was performed using the same cDNA samples as templates with THUNDERBIRD SYBR qPCR Mix (TOYOBO) and gene-specific primers ( ). .. All runs were carried out using MiniOpticon (BIO-RAD) system and the data were analyzed using CFX Manager 3.1 (BIO-RAD) software.

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    Toyobo reverse transcription quantitative pcr rt qpcr
    Results of <t>RT-qPCR</t> for protein coding genes. Gene specific primers for RT-qPCR were mentioned in Table S2 . The detailes of <t>PCR</t> condition for each target gene were descrived in Supporting Information S1 . (A)–(D): Bar graphs showing gene expression relative to gapdh (means; bars = S.D). MH: adult male head (n = 3), MA: adult male abdomen (n = 3), FH: adult female head (n = 3), FA; adult female abdomen (n = 3), cleavage: cleavage-stage embryo (1–8 h post-oviposition, n = 9), morula: primary morula-stage embryo (12–24 h post-oviposition, n = 12). No differences were determined to be statistically significant for any comparison of pairs at p = 0.05 (two-sample t-test or Welch's test).
    Reverse Transcription Quantitative Pcr Rt Qpcr, supplied by Toyobo, used in various techniques. Bioz Stars score: 92/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/reverse transcription quantitative pcr rt qpcr/product/Toyobo
    Average 92 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    reverse transcription quantitative pcr rt qpcr - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

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    Results of RT-qPCR for protein coding genes. Gene specific primers for RT-qPCR were mentioned in Table S2 . The detailes of PCR condition for each target gene were descrived in Supporting Information S1 . (A)–(D): Bar graphs showing gene expression relative to gapdh (means; bars = S.D). MH: adult male head (n = 3), MA: adult male abdomen (n = 3), FH: adult female head (n = 3), FA; adult female abdomen (n = 3), cleavage: cleavage-stage embryo (1–8 h post-oviposition, n = 9), morula: primary morula-stage embryo (12–24 h post-oviposition, n = 12). No differences were determined to be statistically significant for any comparison of pairs at p = 0.05 (two-sample t-test or Welch's test).

    Journal: PLoS ONE

    Article Title: Gene Expression of Protein-Coding and Non-Coding RNAs Related to Polyembryogenesis in the Parasitic Wasp, Copidosoma floridanum

    doi: 10.1371/journal.pone.0114372

    Figure Lengend Snippet: Results of RT-qPCR for protein coding genes. Gene specific primers for RT-qPCR were mentioned in Table S2 . The detailes of PCR condition for each target gene were descrived in Supporting Information S1 . (A)–(D): Bar graphs showing gene expression relative to gapdh (means; bars = S.D). MH: adult male head (n = 3), MA: adult male abdomen (n = 3), FH: adult female head (n = 3), FA; adult female abdomen (n = 3), cleavage: cleavage-stage embryo (1–8 h post-oviposition, n = 9), morula: primary morula-stage embryo (12–24 h post-oviposition, n = 12). No differences were determined to be statistically significant for any comparison of pairs at p = 0.05 (two-sample t-test or Welch's test).

    Article Snippet: Reverse transcription quantitative PCR (RT-qPCR) was performed using the same cDNA samples as templates with THUNDERBIRD SYBR qPCR Mix (TOYOBO) and gene-specific primers ( ).

    Techniques: Quantitative RT-PCR, Polymerase Chain Reaction, Expressing

    Results of RT-qPCR for CflncRNAs. Gene specific primers for RT-qPCR were mentioned in Table S2 . The detailes of PCR condition for each target gene were descrived in Supporting Information S1 . (A)–(C): Bar graphs showing the gene expression relative to gapdh (means, bars = S.D). MH: adult male head (n = 3), MA: adult male abdomen (n = 3), FH: adult female head (n = 3), FA; adult female abdomen (n = 3), cleavage: cleavage-stage embryo (1–8 h post-oviposition, n = 9), morula: primary morula-stage embryo (12–24 h post-oviposition, n = 12). For A and B, *indicates a statistically significant difference, p

    Journal: PLoS ONE

    Article Title: Gene Expression of Protein-Coding and Non-Coding RNAs Related to Polyembryogenesis in the Parasitic Wasp, Copidosoma floridanum

    doi: 10.1371/journal.pone.0114372

    Figure Lengend Snippet: Results of RT-qPCR for CflncRNAs. Gene specific primers for RT-qPCR were mentioned in Table S2 . The detailes of PCR condition for each target gene were descrived in Supporting Information S1 . (A)–(C): Bar graphs showing the gene expression relative to gapdh (means, bars = S.D). MH: adult male head (n = 3), MA: adult male abdomen (n = 3), FH: adult female head (n = 3), FA; adult female abdomen (n = 3), cleavage: cleavage-stage embryo (1–8 h post-oviposition, n = 9), morula: primary morula-stage embryo (12–24 h post-oviposition, n = 12). For A and B, *indicates a statistically significant difference, p

    Article Snippet: Reverse transcription quantitative PCR (RT-qPCR) was performed using the same cDNA samples as templates with THUNDERBIRD SYBR qPCR Mix (TOYOBO) and gene-specific primers ( ).

    Techniques: Quantitative RT-PCR, Polymerase Chain Reaction, Expressing

    PDEF is directly regulated by AR . a AR and PDEF mRNA and protein levels were determined by performing RT-qPCR (top left panels) and western blotting (top right panels), respectively, of MDA-MB-453 and SKBR-3 cells treated with 1 nM DHT for 48 h (+DHT) or without DHT (control). AR and PDEF mRNA and protein levels were determined by performing RT-qPCR (bottom left panels) or western blotting (bottom right panels), respectively, of MDA-MB-453 and SKBR-3 cells infected with a non-specific (NS) shRNA- or AR-shRNA-expressing lentiviral vector (KD: knockdown). Data are presented as mean with SD. b AR and PDEF mRNA levels were determined by performing RT-qPCR of MDA-MB-453 (above) and SKBR-3 (under) cells treated with increasing DHT doses for 48 h. The mRNA levels are presented as mean with SD and have been normalised using those of the housekeeping gene GAPDH . c AR and PDEF protein levels were determined by performing immunofluorescence staining of MDA-MB-453 and SKBR-3 cells treated with 1 nM DHT for 0 h (control) or 48 h (+DHT). AR and PDEF protein levels were determined by performing immunofluorescence staining of MDA-MB-453 and SKBR-3 cells infected with an NS shRNA- or AR-shRNA-expressing lentiviral vector (KD: knockdown). d Co-IP assay was performed with an anti-AR antibody in MDA-MB-453 cells treated with 1 nM DHT for 48 h and control vector-infected cells. The interaction between precipitated PDEF and AR was detected using the anti-AR antibody. e Top panel: Schematic diagram of the AR-binding regions within the PDEF locus. Enh: enhancer. Lower left panel: Results of the direct AR ChIP assay followed by RT-qPCR of MDA-MB-453 cells treated with vehicle (white bars) or 1 nM DHT (48 h, black bars); data are presented as mean ± SD. Lower right panel: Semi-quantitative PCR of a negative control (IgG) sample

    Journal: Molecular Cancer

    Article Title: AR–PDEF pathway promotes tumour proliferation and upregulates MYC-mediated gene transcription by promoting MAD1 degradation in ER-negative breast cancer

    doi: 10.1186/s12943-018-0883-0

    Figure Lengend Snippet: PDEF is directly regulated by AR . a AR and PDEF mRNA and protein levels were determined by performing RT-qPCR (top left panels) and western blotting (top right panels), respectively, of MDA-MB-453 and SKBR-3 cells treated with 1 nM DHT for 48 h (+DHT) or without DHT (control). AR and PDEF mRNA and protein levels were determined by performing RT-qPCR (bottom left panels) or western blotting (bottom right panels), respectively, of MDA-MB-453 and SKBR-3 cells infected with a non-specific (NS) shRNA- or AR-shRNA-expressing lentiviral vector (KD: knockdown). Data are presented as mean with SD. b AR and PDEF mRNA levels were determined by performing RT-qPCR of MDA-MB-453 (above) and SKBR-3 (under) cells treated with increasing DHT doses for 48 h. The mRNA levels are presented as mean with SD and have been normalised using those of the housekeeping gene GAPDH . c AR and PDEF protein levels were determined by performing immunofluorescence staining of MDA-MB-453 and SKBR-3 cells treated with 1 nM DHT for 0 h (control) or 48 h (+DHT). AR and PDEF protein levels were determined by performing immunofluorescence staining of MDA-MB-453 and SKBR-3 cells infected with an NS shRNA- or AR-shRNA-expressing lentiviral vector (KD: knockdown). d Co-IP assay was performed with an anti-AR antibody in MDA-MB-453 cells treated with 1 nM DHT for 48 h and control vector-infected cells. The interaction between precipitated PDEF and AR was detected using the anti-AR antibody. e Top panel: Schematic diagram of the AR-binding regions within the PDEF locus. Enh: enhancer. Lower left panel: Results of the direct AR ChIP assay followed by RT-qPCR of MDA-MB-453 cells treated with vehicle (white bars) or 1 nM DHT (48 h, black bars); data are presented as mean ± SD. Lower right panel: Semi-quantitative PCR of a negative control (IgG) sample

    Article Snippet: Quantitative reverse transcription-PCR (RT-qPCR) was performed using a standard protocol given in SYBR Green PCR kit (Toyobo, Osaka, Japan) and by using iQ5 quantitative PCR system (Bio-Rad, USA).

    Techniques: Quantitative RT-PCR, Western Blot, Multiple Displacement Amplification, Infection, shRNA, Expressing, Plasmid Preparation, Immunofluorescence, Staining, Co-Immunoprecipitation Assay, Binding Assay, Chromatin Immunoprecipitation, Real-time Polymerase Chain Reaction, Negative Control