reverse transcription quantitative pcr rt qpcr  (Stratagene)

 
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    Stratagene reverse transcription quantitative pcr rt qpcr
    Reverse Transcription Quantitative Pcr Rt Qpcr, supplied by Stratagene, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/reverse transcription quantitative pcr rt qpcr/product/Stratagene
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    reverse transcription quantitative pcr rt qpcr - by Bioz Stars, 2020-07
    92/100 stars

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    SYBR Green Assay:

    Article Title: White-nose syndrome is associated with increased replication of a naturally persisting coronaviruses in bats
    Article Snippet: .. Reverse-Transcription Quantitative PCR (RT-qPCR) The Stratagene MX3005P qPCR System was used in conjunction with QuantiFast SYBR Green PCR Kit (Qiagen 204056). ..

    Electrophoresis:

    Article Title: Tumour Microenvironments Induce Expression of Urokinase Plasminogen Activator Receptor (uPAR) and Concomitant Activation of Gelatinolytic Enzymes
    Article Snippet: .. Quantity and purity of the extracted RNA was determined using the NanoDrop spectrophotometer (Thermo Scientific, Wilmington, DE, USA), and RNA integrity was assessed using the Experion automated electrophoresis system (Bio-Rad Laboratories, Hercules, USA). mRNA expression levels were analysed using reverse transcription quantitative PCR (RT-qPCR) on a Stratagene Mx3000P instrument (Stratagene, La Jolla, USA). cDNA was synthesized from 1 µg total RNA using the QuantiTect Reverse Transcription Kit (Qiagen, Hilden, Germany). .. Target cDNA, corresponding to 10 ng RNA, was amplified through 40 cycles in a 25 µl qPCR mix (RT2 SYBR Green/ROX, SA Biosciences, USA) containing 1 µl Qiagen primer mix (uPAR: QT00102984, uPA: QT00103159, Plasminogen: QT01053332, β-actin: QT00095242, and TRFC: QT00122745).

    Synthesized:

    Article Title: Tumour Microenvironments Induce Expression of Urokinase Plasminogen Activator Receptor (uPAR) and Concomitant Activation of Gelatinolytic Enzymes
    Article Snippet: .. Quantity and purity of the extracted RNA was determined using the NanoDrop spectrophotometer (Thermo Scientific, Wilmington, DE, USA), and RNA integrity was assessed using the Experion automated electrophoresis system (Bio-Rad Laboratories, Hercules, USA). mRNA expression levels were analysed using reverse transcription quantitative PCR (RT-qPCR) on a Stratagene Mx3000P instrument (Stratagene, La Jolla, USA). cDNA was synthesized from 1 µg total RNA using the QuantiTect Reverse Transcription Kit (Qiagen, Hilden, Germany). .. Target cDNA, corresponding to 10 ng RNA, was amplified through 40 cycles in a 25 µl qPCR mix (RT2 SYBR Green/ROX, SA Biosciences, USA) containing 1 µl Qiagen primer mix (uPAR: QT00102984, uPA: QT00103159, Plasminogen: QT01053332, β-actin: QT00095242, and TRFC: QT00122745).

    Spectrophotometry:

    Article Title: Tumour Microenvironments Induce Expression of Urokinase Plasminogen Activator Receptor (uPAR) and Concomitant Activation of Gelatinolytic Enzymes
    Article Snippet: .. Quantity and purity of the extracted RNA was determined using the NanoDrop spectrophotometer (Thermo Scientific, Wilmington, DE, USA), and RNA integrity was assessed using the Experion automated electrophoresis system (Bio-Rad Laboratories, Hercules, USA). mRNA expression levels were analysed using reverse transcription quantitative PCR (RT-qPCR) on a Stratagene Mx3000P instrument (Stratagene, La Jolla, USA). cDNA was synthesized from 1 µg total RNA using the QuantiTect Reverse Transcription Kit (Qiagen, Hilden, Germany). .. Target cDNA, corresponding to 10 ng RNA, was amplified through 40 cycles in a 25 µl qPCR mix (RT2 SYBR Green/ROX, SA Biosciences, USA) containing 1 µl Qiagen primer mix (uPAR: QT00102984, uPA: QT00103159, Plasminogen: QT01053332, β-actin: QT00095242, and TRFC: QT00122745).

    Real-time Polymerase Chain Reaction:

    Article Title: Tumour Microenvironments Induce Expression of Urokinase Plasminogen Activator Receptor (uPAR) and Concomitant Activation of Gelatinolytic Enzymes
    Article Snippet: .. Quantity and purity of the extracted RNA was determined using the NanoDrop spectrophotometer (Thermo Scientific, Wilmington, DE, USA), and RNA integrity was assessed using the Experion automated electrophoresis system (Bio-Rad Laboratories, Hercules, USA). mRNA expression levels were analysed using reverse transcription quantitative PCR (RT-qPCR) on a Stratagene Mx3000P instrument (Stratagene, La Jolla, USA). cDNA was synthesized from 1 µg total RNA using the QuantiTect Reverse Transcription Kit (Qiagen, Hilden, Germany). .. Target cDNA, corresponding to 10 ng RNA, was amplified through 40 cycles in a 25 µl qPCR mix (RT2 SYBR Green/ROX, SA Biosciences, USA) containing 1 µl Qiagen primer mix (uPAR: QT00102984, uPA: QT00103159, Plasminogen: QT01053332, β-actin: QT00095242, and TRFC: QT00122745).

    Article Title: White-nose syndrome is associated with increased replication of a naturally persisting coronaviruses in bats
    Article Snippet: .. Reverse-Transcription Quantitative PCR (RT-qPCR) The Stratagene MX3005P qPCR System was used in conjunction with QuantiFast SYBR Green PCR Kit (Qiagen 204056). ..

    Quantitative RT-PCR:

    Article Title: Tumour Microenvironments Induce Expression of Urokinase Plasminogen Activator Receptor (uPAR) and Concomitant Activation of Gelatinolytic Enzymes
    Article Snippet: .. Quantity and purity of the extracted RNA was determined using the NanoDrop spectrophotometer (Thermo Scientific, Wilmington, DE, USA), and RNA integrity was assessed using the Experion automated electrophoresis system (Bio-Rad Laboratories, Hercules, USA). mRNA expression levels were analysed using reverse transcription quantitative PCR (RT-qPCR) on a Stratagene Mx3000P instrument (Stratagene, La Jolla, USA). cDNA was synthesized from 1 µg total RNA using the QuantiTect Reverse Transcription Kit (Qiagen, Hilden, Germany). .. Target cDNA, corresponding to 10 ng RNA, was amplified through 40 cycles in a 25 µl qPCR mix (RT2 SYBR Green/ROX, SA Biosciences, USA) containing 1 µl Qiagen primer mix (uPAR: QT00102984, uPA: QT00103159, Plasminogen: QT01053332, β-actin: QT00095242, and TRFC: QT00122745).

    Article Title: White-nose syndrome is associated with increased replication of a naturally persisting coronaviruses in bats
    Article Snippet: .. Reverse-Transcription Quantitative PCR (RT-qPCR) The Stratagene MX3005P qPCR System was used in conjunction with QuantiFast SYBR Green PCR Kit (Qiagen 204056). ..

    Expressing:

    Article Title: Tumour Microenvironments Induce Expression of Urokinase Plasminogen Activator Receptor (uPAR) and Concomitant Activation of Gelatinolytic Enzymes
    Article Snippet: .. Quantity and purity of the extracted RNA was determined using the NanoDrop spectrophotometer (Thermo Scientific, Wilmington, DE, USA), and RNA integrity was assessed using the Experion automated electrophoresis system (Bio-Rad Laboratories, Hercules, USA). mRNA expression levels were analysed using reverse transcription quantitative PCR (RT-qPCR) on a Stratagene Mx3000P instrument (Stratagene, La Jolla, USA). cDNA was synthesized from 1 µg total RNA using the QuantiTect Reverse Transcription Kit (Qiagen, Hilden, Germany). .. Target cDNA, corresponding to 10 ng RNA, was amplified through 40 cycles in a 25 µl qPCR mix (RT2 SYBR Green/ROX, SA Biosciences, USA) containing 1 µl Qiagen primer mix (uPAR: QT00102984, uPA: QT00103159, Plasminogen: QT01053332, β-actin: QT00095242, and TRFC: QT00122745).

    Polymerase Chain Reaction:

    Article Title: White-nose syndrome is associated with increased replication of a naturally persisting coronaviruses in bats
    Article Snippet: .. Reverse-Transcription Quantitative PCR (RT-qPCR) The Stratagene MX3005P qPCR System was used in conjunction with QuantiFast SYBR Green PCR Kit (Qiagen 204056). ..

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    Stratagene two step quantitative reverse transcription pcr rt qpcr
    LUAT-associated promoters are prone to pervasive transcription. A) Average profiles of Total and PolyA RNA-seq signals in DP thymocytes, for the three set of similarly expressed genes. Signals coming from plus and minus strands are indicated by solid and dashed lines, respectively. B) Splicing index calculated for the 5′ and middle exons for the three set of similarly expressed genes in DP thymocytes. C) Boxplots showing the density of Total RNA-seq reads per bp in the same orientation as the matched coding genes and within the first intron of the three group of genes in DP thymocytes. Statistical significance was assessed by the Mann–Whitney U test. D) Intron/exon ratio of individual genes for the three gene sets in DP thymocytes assessed by reverse transcription quantitative <t>PCR.</t> Relative transcript levels at the first intron and the last exon of each gene was estimated based on a standard dilution of genomic DNA. Statistical significance were assessed using Wilcoxon rank sum tests. E) Schematic representation of RNA processing at the three different classes of gene loci. Exons are shown by rectangles (or stripped rectangles in the case of LUATs). Solid and dotted lines represent immature (unspliced) and processed (spliced) transcripts, respectively. Our results suggest that LUAT-associated genes display an increased rate of immature transcripts.
    Two Step Quantitative Reverse Transcription Pcr Rt Qpcr, supplied by Stratagene, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/two step quantitative reverse transcription pcr rt qpcr/product/Stratagene
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    two step quantitative reverse transcription pcr rt qpcr - by Bioz Stars, 2020-07
    85/100 stars
      Buy from Supplier

    90
    Stratagene quantitative reverse transcription pcr rt qpcr rt qpcr
    LUAT-associated promoters are prone to pervasive transcription. A) Average profiles of Total and PolyA RNA-seq signals in DP thymocytes, for the three set of similarly expressed genes. Signals coming from plus and minus strands are indicated by solid and dashed lines, respectively. B) Splicing index calculated for the 5′ and middle exons for the three set of similarly expressed genes in DP thymocytes. C) Boxplots showing the density of Total RNA-seq reads per bp in the same orientation as the matched coding genes and within the first intron of the three group of genes in DP thymocytes. Statistical significance was assessed by the Mann–Whitney U test. D) Intron/exon ratio of individual genes for the three gene sets in DP thymocytes assessed by reverse transcription quantitative <t>PCR.</t> Relative transcript levels at the first intron and the last exon of each gene was estimated based on a standard dilution of genomic DNA. Statistical significance were assessed using Wilcoxon rank sum tests. E) Schematic representation of RNA processing at the three different classes of gene loci. Exons are shown by rectangles (or stripped rectangles in the case of LUATs). Solid and dotted lines represent immature (unspliced) and processed (spliced) transcripts, respectively. Our results suggest that LUAT-associated genes display an increased rate of immature transcripts.
    Quantitative Reverse Transcription Pcr Rt Qpcr Rt Qpcr, supplied by Stratagene, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quantitative reverse transcription pcr rt qpcr rt qpcr/product/Stratagene
    Average 90 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    quantitative reverse transcription pcr rt qpcr rt qpcr - by Bioz Stars, 2020-07
    90/100 stars
      Buy from Supplier

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    LUAT-associated promoters are prone to pervasive transcription. A) Average profiles of Total and PolyA RNA-seq signals in DP thymocytes, for the three set of similarly expressed genes. Signals coming from plus and minus strands are indicated by solid and dashed lines, respectively. B) Splicing index calculated for the 5′ and middle exons for the three set of similarly expressed genes in DP thymocytes. C) Boxplots showing the density of Total RNA-seq reads per bp in the same orientation as the matched coding genes and within the first intron of the three group of genes in DP thymocytes. Statistical significance was assessed by the Mann–Whitney U test. D) Intron/exon ratio of individual genes for the three gene sets in DP thymocytes assessed by reverse transcription quantitative PCR. Relative transcript levels at the first intron and the last exon of each gene was estimated based on a standard dilution of genomic DNA. Statistical significance were assessed using Wilcoxon rank sum tests. E) Schematic representation of RNA processing at the three different classes of gene loci. Exons are shown by rectangles (or stripped rectangles in the case of LUATs). Solid and dotted lines represent immature (unspliced) and processed (spliced) transcripts, respectively. Our results suggest that LUAT-associated genes display an increased rate of immature transcripts.

    Journal: BMC Genomics

    Article Title: Divergent transcription is associated with promoters of transcriptional regulators

    doi: 10.1186/1471-2164-14-914

    Figure Lengend Snippet: LUAT-associated promoters are prone to pervasive transcription. A) Average profiles of Total and PolyA RNA-seq signals in DP thymocytes, for the three set of similarly expressed genes. Signals coming from plus and minus strands are indicated by solid and dashed lines, respectively. B) Splicing index calculated for the 5′ and middle exons for the three set of similarly expressed genes in DP thymocytes. C) Boxplots showing the density of Total RNA-seq reads per bp in the same orientation as the matched coding genes and within the first intron of the three group of genes in DP thymocytes. Statistical significance was assessed by the Mann–Whitney U test. D) Intron/exon ratio of individual genes for the three gene sets in DP thymocytes assessed by reverse transcription quantitative PCR. Relative transcript levels at the first intron and the last exon of each gene was estimated based on a standard dilution of genomic DNA. Statistical significance were assessed using Wilcoxon rank sum tests. E) Schematic representation of RNA processing at the three different classes of gene loci. Exons are shown by rectangles (or stripped rectangles in the case of LUATs). Solid and dotted lines represent immature (unspliced) and processed (spliced) transcripts, respectively. Our results suggest that LUAT-associated genes display an increased rate of immature transcripts.

    Article Snippet: Two-step quantitative reverse transcription PCR (RT-qPCR) was performed using the Stratagene Mx3000P Sequence Detection System.

    Techniques: RNA Sequencing Assay, MANN-WHITNEY, Real-time Polymerase Chain Reaction