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    TaKaRa reverse transcription quantitative pcr rt qpcr
    SpoVG represses the transcription of sasC . (A) The transcriptional levels of 17 genes encoding extracellular proteins in the WT and spoVG -deletion strains were determined by <t>RT-qPCR</t> (OD 600 of 2). (B) The transcriptional levels of sasC in the WT, spoVG -deletion, and spoVG -complemented strains were determined by RT-qPCR (OD 600 of 6). (C) The β-galactosidase activity of sasC in the WT and spoVG -deletion strains. Bacterial cells were collected at an OD 600 of 6, and the β-galactosidase activity was detected with the substrate ONPG. The spoVG -deletion strain carrying pOS rot was used as a positive control and PBS as a negative control. (D) EMSA of nonphosphorylated SpoVG (SpoVG) or the hyperphosphorylated SpoVG (SpoVG-P) with the biotin-labeled promoter p sasC . The promoter region of sasC was amplified by <t>PCR</t> and incubated with purified SpoVG (top) or SpoVG-P (bottom). The unlabeled probe was used as the specific competitor and the unlabeled partial fragment of the hu ORF region as the nonspecific competitor. (E) The transcriptional levels of ebhB in the WT, spoVG -deletion, and spoVG -complemented strains were determined by RT-qPCR (OD 600 of 6). Values represent results from three biological replicates ± SEM. Statistical values were determined by the use of the Student's t test and the F test to compare variances. *, P
    Reverse Transcription Quantitative Pcr Rt Qpcr, supplied by TaKaRa, used in various techniques. Bioz Stars score: 92/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/reverse transcription quantitative pcr rt qpcr/product/TaKaRa
    Average 92 stars, based on 26 article reviews
    Price from $9.99 to $1999.99
    reverse transcription quantitative pcr rt qpcr - by Bioz Stars, 2020-09
    92/100 stars

    Images

    1) Product Images from "SpoVG Modulates Cell Aggregation in Staphylococcus aureus by Regulating sasC Expression and Extracellular DNA Release"

    Article Title: SpoVG Modulates Cell Aggregation in Staphylococcus aureus by Regulating sasC Expression and Extracellular DNA Release

    Journal: Applied and Environmental Microbiology

    doi: 10.1128/AEM.00591-20

    SpoVG represses the transcription of sasC . (A) The transcriptional levels of 17 genes encoding extracellular proteins in the WT and spoVG -deletion strains were determined by RT-qPCR (OD 600 of 2). (B) The transcriptional levels of sasC in the WT, spoVG -deletion, and spoVG -complemented strains were determined by RT-qPCR (OD 600 of 6). (C) The β-galactosidase activity of sasC in the WT and spoVG -deletion strains. Bacterial cells were collected at an OD 600 of 6, and the β-galactosidase activity was detected with the substrate ONPG. The spoVG -deletion strain carrying pOS rot was used as a positive control and PBS as a negative control. (D) EMSA of nonphosphorylated SpoVG (SpoVG) or the hyperphosphorylated SpoVG (SpoVG-P) with the biotin-labeled promoter p sasC . The promoter region of sasC was amplified by PCR and incubated with purified SpoVG (top) or SpoVG-P (bottom). The unlabeled probe was used as the specific competitor and the unlabeled partial fragment of the hu ORF region as the nonspecific competitor. (E) The transcriptional levels of ebhB in the WT, spoVG -deletion, and spoVG -complemented strains were determined by RT-qPCR (OD 600 of 6). Values represent results from three biological replicates ± SEM. Statistical values were determined by the use of the Student's t test and the F test to compare variances. *, P
    Figure Legend Snippet: SpoVG represses the transcription of sasC . (A) The transcriptional levels of 17 genes encoding extracellular proteins in the WT and spoVG -deletion strains were determined by RT-qPCR (OD 600 of 2). (B) The transcriptional levels of sasC in the WT, spoVG -deletion, and spoVG -complemented strains were determined by RT-qPCR (OD 600 of 6). (C) The β-galactosidase activity of sasC in the WT and spoVG -deletion strains. Bacterial cells were collected at an OD 600 of 6, and the β-galactosidase activity was detected with the substrate ONPG. The spoVG -deletion strain carrying pOS rot was used as a positive control and PBS as a negative control. (D) EMSA of nonphosphorylated SpoVG (SpoVG) or the hyperphosphorylated SpoVG (SpoVG-P) with the biotin-labeled promoter p sasC . The promoter region of sasC was amplified by PCR and incubated with purified SpoVG (top) or SpoVG-P (bottom). The unlabeled probe was used as the specific competitor and the unlabeled partial fragment of the hu ORF region as the nonspecific competitor. (E) The transcriptional levels of ebhB in the WT, spoVG -deletion, and spoVG -complemented strains were determined by RT-qPCR (OD 600 of 6). Values represent results from three biological replicates ± SEM. Statistical values were determined by the use of the Student's t test and the F test to compare variances. *, P

    Techniques Used: Quantitative RT-PCR, Activity Assay, Positive Control, Negative Control, Labeling, Amplification, Polymerase Chain Reaction, Incubation, Purification

    2) Product Images from "The fine‐scale genetic structure and selection signals of Chinese indigenous pigs, et al. The fine‐scale genetic structure and selection signals of Chinese indigenous pigs"

    Article Title: The fine‐scale genetic structure and selection signals of Chinese indigenous pigs, et al. The fine‐scale genetic structure and selection signals of Chinese indigenous pigs

    Journal: Evolutionary Applications

    doi: 10.1111/eva.12887

    EDNRB transcripts and candidate causative mutation for the two‐end‐black coat color phenotype in pigs. (a) EDNRB transcripts in the skin of Bamaxiang and Jinhua pigs. RNA sequencing reads are aligned to the genomic sequence of EDNRB in the pig reference genome. The orange shaded region denotes alternative transcripts between Bamaxiang and Jinhua pigs. BMX_BL, Bamaxiang black skin; BMX_WH, Bamaxiang white skin; JH_BL, Jinhua black skin; JH_WH, Jinhua white skin (b) Whole‐length isoform transcripts in the skin of Bamaxiang (BMX), Jinhua (JH) and DLY pigs revealed by Iso‐seq. (c) RT‐PCR and RT‐qPCR of EDNRB transcripts in the skin of Bamaxiang (BMX) and Jinhua (JH) pigs. RT‐qPCR result is shown in the right panel and the y‐axis represents relative expression level (alternative splicing/reference). (d) Schematic diagram of EDNRB transcripts and their encoded protein. The DNA sequences (chromosome 11:50,076,938–50,076,994 bp) in the reference genome and Bamaxiang pigs are shown in the upper panel. The normal and alternative transcripts and their encoded proteins of EDNRB are shown in the middle and lower panels, respectively. The capital and lowercase represent exonic and intronic regions, respectively. The orange arrow indicates transcription direction (top). The red triangle or underline indicates the 11‐bp Indel that causes premature stop codon of the EDNRB alternative transcript in Bamaxiang pigs. (e) Predicted three‐dimensional protein structures of EDNRB encoded by the normal (left) and alternative (right) transcripts. (f) Allele frequencies of the 11‐bp deletion, the candidate causative mutation in the EDNRB gene (chromosome 11: 50,076,945–50,076,960 bp), in global pig breeds and wild boars. 1, three Chinese two‐end‐black breeds including Bamaxiang, Luchuan, and Pingxiang pigs
    Figure Legend Snippet: EDNRB transcripts and candidate causative mutation for the two‐end‐black coat color phenotype in pigs. (a) EDNRB transcripts in the skin of Bamaxiang and Jinhua pigs. RNA sequencing reads are aligned to the genomic sequence of EDNRB in the pig reference genome. The orange shaded region denotes alternative transcripts between Bamaxiang and Jinhua pigs. BMX_BL, Bamaxiang black skin; BMX_WH, Bamaxiang white skin; JH_BL, Jinhua black skin; JH_WH, Jinhua white skin (b) Whole‐length isoform transcripts in the skin of Bamaxiang (BMX), Jinhua (JH) and DLY pigs revealed by Iso‐seq. (c) RT‐PCR and RT‐qPCR of EDNRB transcripts in the skin of Bamaxiang (BMX) and Jinhua (JH) pigs. RT‐qPCR result is shown in the right panel and the y‐axis represents relative expression level (alternative splicing/reference). (d) Schematic diagram of EDNRB transcripts and their encoded protein. The DNA sequences (chromosome 11:50,076,938–50,076,994 bp) in the reference genome and Bamaxiang pigs are shown in the upper panel. The normal and alternative transcripts and their encoded proteins of EDNRB are shown in the middle and lower panels, respectively. The capital and lowercase represent exonic and intronic regions, respectively. The orange arrow indicates transcription direction (top). The red triangle or underline indicates the 11‐bp Indel that causes premature stop codon of the EDNRB alternative transcript in Bamaxiang pigs. (e) Predicted three‐dimensional protein structures of EDNRB encoded by the normal (left) and alternative (right) transcripts. (f) Allele frequencies of the 11‐bp deletion, the candidate causative mutation in the EDNRB gene (chromosome 11: 50,076,945–50,076,960 bp), in global pig breeds and wild boars. 1, three Chinese two‐end‐black breeds including Bamaxiang, Luchuan, and Pingxiang pigs

    Techniques Used: Mutagenesis, RNA Sequencing Assay, Sequencing, Reverse Transcription Polymerase Chain Reaction, Quantitative RT-PCR, Expressing

    3) Product Images from "Fibroblast-derived exosomal microRNA-369 potentiates migration and invasion of lung squamous cell carcinoma cells via NF1-mediated MAPK signaling pathway"

    Article Title: Fibroblast-derived exosomal microRNA-369 potentiates migration and invasion of lung squamous cell carcinoma cells via NF1-mediated MAPK signaling pathway

    Journal: International Journal of Molecular Medicine

    doi: 10.3892/ijmm.2020.4614

    CAFs-derived EVs upregulate miR-369 expression in LUSC cells. (A) Dysregulated miRNAs following EVs treatment predicted using miRCURY LNA™ Universal RT microRNA PCR Human panel. (B) The miR-369 expression in all tumor types. (C) miR-369 expression in H520 and SK-MES-1 cells following EVs treatment measured by RT-qPCR. (D) The miR-369 expression in patients with LUSC and normal individuals in The Cancer Genome Atlas data-sets. (E) The miR-369 expression in 52 LUSC tumor tissues and paracancerous tissues measured by RT-qPCR. (F) miR-369 expression in CAFs and its EVs treated with miR-369 inhibitor or inhibitor control. Data are expressed as mean ± SD. In panels C and F, one-way analysis of variance and Tukey's multiple comparisons test was used to determine statistical significance. * P
    Figure Legend Snippet: CAFs-derived EVs upregulate miR-369 expression in LUSC cells. (A) Dysregulated miRNAs following EVs treatment predicted using miRCURY LNA™ Universal RT microRNA PCR Human panel. (B) The miR-369 expression in all tumor types. (C) miR-369 expression in H520 and SK-MES-1 cells following EVs treatment measured by RT-qPCR. (D) The miR-369 expression in patients with LUSC and normal individuals in The Cancer Genome Atlas data-sets. (E) The miR-369 expression in 52 LUSC tumor tissues and paracancerous tissues measured by RT-qPCR. (F) miR-369 expression in CAFs and its EVs treated with miR-369 inhibitor or inhibitor control. Data are expressed as mean ± SD. In panels C and F, one-way analysis of variance and Tukey's multiple comparisons test was used to determine statistical significance. * P

    Techniques Used: Derivative Assay, Expressing, Polymerase Chain Reaction, Quantitative RT-PCR

    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: The relationship between liver-kidney impairment and viral load after nephropathogenic infectious bronchitis virus infection in embryonic chickens.
    Article Snippet: .. Determination of the Liver and Kidney Viral Loads with Reverse-Transcription Quantitative PCR (RT-qPCR) Before the reverse transcription reaction, all of the RNA samples were individually treated with genomic DNA elimination reagent according to the manufacturer's instructions to remove the genomic DNA (Takara, Dalian, China). .. For reverse transcription, 10 μL of total reaction mixture (1.0 μL of gDNA Eraser, 2.0 μL of 5× gDNA Eraser Buffer, 0.1 μg of total RNA, and 10 μL of RNase-free dH 2 O) was incubated at 42 • C for 2 min and stored at 4 • C. The cDNA was synthesized via a reverse transcription reaction that was performed in a reaction volume of 20 μL that contained the following: the reaction solution formed from the genomic DNA elimination reaction (10 μL), RT Primer Mix (4.0 μL), 5× PrimeScript Buffer 2 (4.0 μL), and PrimeScript RT Enzyme Mix 1 (1.0 μL), RNase-free dH 2 O (1.0 μL).

    Article Title: The Arabidopsis Hypoxia Inducible AtR8 Long Non-Coding RNA also Contributes to Plant Defense and Root Elongation Coordinating with WRKY Genes under Low Levels of Salicylic Acid
    Article Snippet: .. Reverse transcription quantitative PCR (RT-qPCR) was performed using a One-Step SYBR PrimeScript RT-PCR Plus Kit (Takara Bio) and the Eco Real-Time PCR System (Illumina) in 10 µL reactions containing 50 ng or 100 ng of total RNA. .. Reverse transcription was carried out at 42 °C for 5 min, followed by 40 cycles at 95 °C for 5 s and 60 °C for 30 s. A comparative quantitation was carried out via the 2(−∆Ct) method or 2(−∆∆Ct) method [ ].

    Article Title: SpoVG Modulates Cell Aggregation in Staphylococcus aureus by Regulating sasC Expression and Extracellular DNA Release
    Article Snippet: .. Reverse transcription-quantitative PCR (RT-qPCR) was performed with SYBR Premix Ex Taq (TaKaRa) using a StepOne real-time PCR system (Applied Biosystems) and LC96 real-time PCR system (Roche). ..

    Article Title: The fine‐scale genetic structure and selection signals of Chinese indigenous pigs, et al. The fine‐scale genetic structure and selection signals of Chinese indigenous pigs
    Article Snippet: .. To quantify the relative contents of EDNRB alternative transcripts in skin tissues of Bamaxiang, Jinhua, and DLY pigs, reverse transcription quantitative PCR (RT‐qPCR) was performed using SYBR Premix Ex Taq II kit (TaKaRa). ..

    Transfection:

    Article Title: miR-10a-5p inhibits osteogenic differentiation of bone marrow-derived mesenchymal stem cells
    Article Snippet: .. Reverse transcription-quantitative PCR (RT-qPCR) For RT-qPCR analysis, total RNA was extracted from transfected hBMSCs with RNA Iso-Plus reagent (Takara Bio, Inc.). .. RT-qPCR was performed using the primers listed in (Takara Bio, Inc.).

    Quantitative RT-PCR:

    Article Title: The relationship between liver-kidney impairment and viral load after nephropathogenic infectious bronchitis virus infection in embryonic chickens.
    Article Snippet: .. Determination of the Liver and Kidney Viral Loads with Reverse-Transcription Quantitative PCR (RT-qPCR) Before the reverse transcription reaction, all of the RNA samples were individually treated with genomic DNA elimination reagent according to the manufacturer's instructions to remove the genomic DNA (Takara, Dalian, China). .. For reverse transcription, 10 μL of total reaction mixture (1.0 μL of gDNA Eraser, 2.0 μL of 5× gDNA Eraser Buffer, 0.1 μg of total RNA, and 10 μL of RNase-free dH 2 O) was incubated at 42 • C for 2 min and stored at 4 • C. The cDNA was synthesized via a reverse transcription reaction that was performed in a reaction volume of 20 μL that contained the following: the reaction solution formed from the genomic DNA elimination reaction (10 μL), RT Primer Mix (4.0 μL), 5× PrimeScript Buffer 2 (4.0 μL), and PrimeScript RT Enzyme Mix 1 (1.0 μL), RNase-free dH 2 O (1.0 μL).

    Article Title: Fibroblast-derived exosomal microRNA-369 potentiates migration and invasion of lung squamous cell carcinoma cells via NF1-mediated MAPK signaling pathway
    Article Snippet: .. Reverse transcription-quantitative PCR (RT-qPCR) The total RNA was extracted from tissues and cells using RNAiso Plus (Takara Bio, Inc.) or TRIzol® LS Reagent (Takara Bio, Inc.). .. Subsequently, 10 mg RNA was subjected to a 30-min at 10 V/cm voltage using 2% agarose gel at room temperature to verify the reliability of RNA.

    Article Title: miR-10a-5p inhibits osteogenic differentiation of bone marrow-derived mesenchymal stem cells
    Article Snippet: .. Reverse transcription-quantitative PCR (RT-qPCR) For RT-qPCR analysis, total RNA was extracted from transfected hBMSCs with RNA Iso-Plus reagent (Takara Bio, Inc.). .. RT-qPCR was performed using the primers listed in (Takara Bio, Inc.).

    Article Title: The Arabidopsis Hypoxia Inducible AtR8 Long Non-Coding RNA also Contributes to Plant Defense and Root Elongation Coordinating with WRKY Genes under Low Levels of Salicylic Acid
    Article Snippet: .. Reverse transcription quantitative PCR (RT-qPCR) was performed using a One-Step SYBR PrimeScript RT-PCR Plus Kit (Takara Bio) and the Eco Real-Time PCR System (Illumina) in 10 µL reactions containing 50 ng or 100 ng of total RNA. .. Reverse transcription was carried out at 42 °C for 5 min, followed by 40 cycles at 95 °C for 5 s and 60 °C for 30 s. A comparative quantitation was carried out via the 2(−∆Ct) method or 2(−∆∆Ct) method [ ].

    Article Title: SpoVG Modulates Cell Aggregation in Staphylococcus aureus by Regulating sasC Expression and Extracellular DNA Release
    Article Snippet: .. Reverse transcription-quantitative PCR (RT-qPCR) was performed with SYBR Premix Ex Taq (TaKaRa) using a StepOne real-time PCR system (Applied Biosystems) and LC96 real-time PCR system (Roche). ..

    Article Title: The Cytoprotective Enzyme Heme Oxygenase-1 Suppresses Pseudorabies Virus Replication in vitro
    Article Snippet: .. Reverse Transcription-Quantitative PCR (RT-qPCR) Following treatment, cells were harvested and total RNA were extracted using RNAiso plus reagent (Takara Biotechnology Co., Ltd.), according to the manufacturer’s protocol. .. Total RNA (500 ng) was reverse transcribed into cDNA using the PrimeScript® RT reagent kit (Takara Biotechnology Co., Ltd.). qPCR was subsequently performed on a Light Cycler 480 SYBR Green I Master fluorescence quantitative PCR instrument, using FastStart Universal SYBR green master (Roche Diagnostics GmbH).

    Article Title: The fine‐scale genetic structure and selection signals of Chinese indigenous pigs, et al. The fine‐scale genetic structure and selection signals of Chinese indigenous pigs
    Article Snippet: .. To quantify the relative contents of EDNRB alternative transcripts in skin tissues of Bamaxiang, Jinhua, and DLY pigs, reverse transcription quantitative PCR (RT‐qPCR) was performed using SYBR Premix Ex Taq II kit (TaKaRa). ..

    SYBR Green Assay:

    Article Title: Expression of Notch family is altered in non-alcoholic fatty liver disease
    Article Snippet: .. Reverse transcription-quantitative PCR (RT-qPCR) was performed using a SYBR Green kit (Takara Biotechnology Co., Ltd.) in an ABI 7500 Real-Time PCR system (Applied Biosystems; Thermo Fisher Scientific, Inc.). .. The standard conditions for qPCR were: 50°C for 10 min and 95°C for 15 and 45 sec annealing/elongation at 58°C or 60°C.

    Polymerase Chain Reaction:

    Article Title: Fibroblast-derived exosomal microRNA-369 potentiates migration and invasion of lung squamous cell carcinoma cells via NF1-mediated MAPK signaling pathway
    Article Snippet: .. Reverse transcription-quantitative PCR (RT-qPCR) The total RNA was extracted from tissues and cells using RNAiso Plus (Takara Bio, Inc.) or TRIzol® LS Reagent (Takara Bio, Inc.). .. Subsequently, 10 mg RNA was subjected to a 30-min at 10 V/cm voltage using 2% agarose gel at room temperature to verify the reliability of RNA.

    Article Title: miR-10a-5p inhibits osteogenic differentiation of bone marrow-derived mesenchymal stem cells
    Article Snippet: .. Reverse transcription-quantitative PCR (RT-qPCR) For RT-qPCR analysis, total RNA was extracted from transfected hBMSCs with RNA Iso-Plus reagent (Takara Bio, Inc.). .. RT-qPCR was performed using the primers listed in (Takara Bio, Inc.).

    Article Title: SpoVG Modulates Cell Aggregation in Staphylococcus aureus by Regulating sasC Expression and Extracellular DNA Release
    Article Snippet: .. Reverse transcription-quantitative PCR (RT-qPCR) was performed with SYBR Premix Ex Taq (TaKaRa) using a StepOne real-time PCR system (Applied Biosystems) and LC96 real-time PCR system (Roche). ..

    Article Title: The Cytoprotective Enzyme Heme Oxygenase-1 Suppresses Pseudorabies Virus Replication in vitro
    Article Snippet: .. Reverse Transcription-Quantitative PCR (RT-qPCR) Following treatment, cells were harvested and total RNA were extracted using RNAiso plus reagent (Takara Biotechnology Co., Ltd.), according to the manufacturer’s protocol. .. Total RNA (500 ng) was reverse transcribed into cDNA using the PrimeScript® RT reagent kit (Takara Biotechnology Co., Ltd.). qPCR was subsequently performed on a Light Cycler 480 SYBR Green I Master fluorescence quantitative PCR instrument, using FastStart Universal SYBR green master (Roche Diagnostics GmbH).

    Article Title: Expression of Notch family is altered in non-alcoholic fatty liver disease
    Article Snippet: .. Reverse transcription-quantitative PCR (RT-qPCR) was performed using a SYBR Green kit (Takara Biotechnology Co., Ltd.) in an ABI 7500 Real-Time PCR system (Applied Biosystems; Thermo Fisher Scientific, Inc.). .. The standard conditions for qPCR were: 50°C for 10 min and 95°C for 15 and 45 sec annealing/elongation at 58°C or 60°C.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: The Arabidopsis Hypoxia Inducible AtR8 Long Non-Coding RNA also Contributes to Plant Defense and Root Elongation Coordinating with WRKY Genes under Low Levels of Salicylic Acid
    Article Snippet: .. Reverse transcription quantitative PCR (RT-qPCR) was performed using a One-Step SYBR PrimeScript RT-PCR Plus Kit (Takara Bio) and the Eco Real-Time PCR System (Illumina) in 10 µL reactions containing 50 ng or 100 ng of total RNA. .. Reverse transcription was carried out at 42 °C for 5 min, followed by 40 cycles at 95 °C for 5 s and 60 °C for 30 s. A comparative quantitation was carried out via the 2(−∆Ct) method or 2(−∆∆Ct) method [ ].

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  • 92
    TaKaRa reverse transcription quantitative pcr rt qpcr total rna
    <t>RT-PCR</t> analysis for GSK-3β mRNA and <t>RT-qPCR</t> for β-catenin mRNA. (A) Following 35 cycles, 5 µ l samples were separated electrophoretically through the agarose gel, and the expected product at 378 base pairs for GSK-3 β were stained with ethidium bromide. Lane 1, control ESC; lane 2, DKK1-treated ESCs; lane 3, teratoma tissue derived from control ESCs; lane 4, teratoma tissue derived from DKK1-treated ESCs. (B) Histogram of GSK-3β mRNA expression by RT-PCR. (C) Melt curve of RT-qPCR for β-catenin. (D) Histogram for β-catenin by RT-qPCR. * P
    Reverse Transcription Quantitative Pcr Rt Qpcr Total Rna, supplied by TaKaRa, used in various techniques. Bioz Stars score: 92/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/reverse transcription quantitative pcr rt qpcr total rna/product/TaKaRa
    Average 92 stars, based on 22 article reviews
    Price from $9.99 to $1999.99
    reverse transcription quantitative pcr rt qpcr total rna - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    91
    TaKaRa reverse transcription quantitative pcr rt qpcr real time quantitative pcr analysis
    Real-time quantitative <t>PCR</t> analysis and model of Chr11 in YZG-5. ( a ) Diagram of Chr11 with 21 related genes uniformly distributed on the short arm and <t>qPCR</t> results. The level of gene expression differed almost two-fold between Os11g01200-11g10130 and Os11g10120-11g20790. ( b ) Model pattern of the composition of Chr11 in variant YZG-5.
    Reverse Transcription Quantitative Pcr Rt Qpcr Real Time Quantitative Pcr Analysis, supplied by TaKaRa, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/reverse transcription quantitative pcr rt qpcr real time quantitative pcr analysis/product/TaKaRa
    Average 91 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    reverse transcription quantitative pcr rt qpcr real time quantitative pcr analysis - by Bioz Stars, 2020-09
    91/100 stars
      Buy from Supplier

    90
    TaKaRa reverse transcription quantitative pcr rt qpcr total mrna
    Expression of retinoblastoma (RB) in vitro and in vivo. (A) Detection of RB94 in A549 cells by western blot analysis. (B) Reverse transcription-quantitative polymerase chain reaction analysis of RB94 <t>mRNA</t> levels in vivo . Data are expressed as the means ± SD (n=3). ** P
    Reverse Transcription Quantitative Pcr Rt Qpcr Total Mrna, supplied by TaKaRa, used in various techniques. Bioz Stars score: 90/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/reverse transcription quantitative pcr rt qpcr total mrna/product/TaKaRa
    Average 90 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    reverse transcription quantitative pcr rt qpcr total mrna - by Bioz Stars, 2020-09
    90/100 stars
      Buy from Supplier

    Image Search Results


    RT-PCR analysis for GSK-3β mRNA and RT-qPCR for β-catenin mRNA. (A) Following 35 cycles, 5 µ l samples were separated electrophoretically through the agarose gel, and the expected product at 378 base pairs for GSK-3 β were stained with ethidium bromide. Lane 1, control ESC; lane 2, DKK1-treated ESCs; lane 3, teratoma tissue derived from control ESCs; lane 4, teratoma tissue derived from DKK1-treated ESCs. (B) Histogram of GSK-3β mRNA expression by RT-PCR. (C) Melt curve of RT-qPCR for β-catenin. (D) Histogram for β-catenin by RT-qPCR. * P

    Journal: Molecular Medicine Reports

    Article Title: Dickkopf Wnt signaling pathway inhibitor 1 regulates the differentiation of mouse embryonic stem cells in vitro and in vivo

    doi: 10.3892/mmr.2015.4586

    Figure Lengend Snippet: RT-PCR analysis for GSK-3β mRNA and RT-qPCR for β-catenin mRNA. (A) Following 35 cycles, 5 µ l samples were separated electrophoretically through the agarose gel, and the expected product at 378 base pairs for GSK-3 β were stained with ethidium bromide. Lane 1, control ESC; lane 2, DKK1-treated ESCs; lane 3, teratoma tissue derived from control ESCs; lane 4, teratoma tissue derived from DKK1-treated ESCs. (B) Histogram of GSK-3β mRNA expression by RT-PCR. (C) Melt curve of RT-qPCR for β-catenin. (D) Histogram for β-catenin by RT-qPCR. * P

    Article Snippet: Reverse transcription-quantitative PCR (RT-qPCR) Total RNA was extracted from cells using TRIzol reagent according to the manufacturer's instructions and cDNA was generated using a reverse transcription kit (Takara Bio, Inc.) according to the manufacturer's instructions.

    Techniques: Reverse Transcription Polymerase Chain Reaction, Quantitative RT-PCR, Agarose Gel Electrophoresis, Staining, Derivative Assay, Expressing

    Real-time quantitative PCR analysis and model of Chr11 in YZG-5. ( a ) Diagram of Chr11 with 21 related genes uniformly distributed on the short arm and qPCR results. The level of gene expression differed almost two-fold between Os11g01200-11g10130 and Os11g10120-11g20790. ( b ) Model pattern of the composition of Chr11 in variant YZG-5.

    Journal: Scientific Reports

    Article Title: Segmental Duplication of Chromosome 11 and its Implications for Cell Division and Genome-wide Expression in Rice

    doi: 10.1038/s41598-017-02796-9

    Figure Lengend Snippet: Real-time quantitative PCR analysis and model of Chr11 in YZG-5. ( a ) Diagram of Chr11 with 21 related genes uniformly distributed on the short arm and qPCR results. The level of gene expression differed almost two-fold between Os11g01200-11g10130 and Os11g10120-11g20790. ( b ) Model pattern of the composition of Chr11 in variant YZG-5.

    Article Snippet: Real-time quantitative PCR (qPCR) and reverse transcription quantitative PCR (RT-qPCR) Real-time quantitative PCR analysis was performed using the ABI ViiATM Real Time Quantitative PCR System with SYBR Premix Ex Taq (TaKaRa) and gene-specific primers (Table ).

    Techniques: Real-time Polymerase Chain Reaction, Expressing, Variant Assay

    Expressional regulation of enzyme genes and regulatory genes by TP05746 in T. pinophilus as demonstrated by RT-qPCR. (A) Amylase genes and their regulatory genes. Total RNA was extracted from fungal strains cultured in medium containing SCS as the carbon source for 12, 24, and 48 h after transfer from glucose. (B) Cellulase and xylanase genes. (C) Genes involved in conidiogenesis. Fungal cells were cultured on WA for 12, 24, and 48 h after transfer from glucose. Expression levels of the genes tested in the Δ TP05746 mutant were normalized against the parental strain Δ TpKu70 . ∗∗ p ≤ 0.01 and ∗ p ≤ 0.05 indicate differences between Δ TP05746 and Δ TpKu70 by Student’s t test. Each experiment contained three biological replicates. RT-qPCR, real-time quantitative reverse transcription PCR; WA, wheat bran plus Avicel.

    Journal: Frontiers in Microbiology

    Article Title: Identification of a Novel Transcription Factor TP05746 Involved in Regulating the Production of Plant-Biomass-Degrading Enzymes in Talaromyces pinophilus

    doi: 10.3389/fmicb.2019.02875

    Figure Lengend Snippet: Expressional regulation of enzyme genes and regulatory genes by TP05746 in T. pinophilus as demonstrated by RT-qPCR. (A) Amylase genes and their regulatory genes. Total RNA was extracted from fungal strains cultured in medium containing SCS as the carbon source for 12, 24, and 48 h after transfer from glucose. (B) Cellulase and xylanase genes. (C) Genes involved in conidiogenesis. Fungal cells were cultured on WA for 12, 24, and 48 h after transfer from glucose. Expression levels of the genes tested in the Δ TP05746 mutant were normalized against the parental strain Δ TpKu70 . ∗∗ p ≤ 0.01 and ∗ p ≤ 0.05 indicate differences between Δ TP05746 and Δ TpKu70 by Student’s t test. Each experiment contained three biological replicates. RT-qPCR, real-time quantitative reverse transcription PCR; WA, wheat bran plus Avicel.

    Article Snippet: Real-Time Quantitative Reverse Transcription-PCR (RT-qPCR) Analysis The PrimeScriptTM RT Reagent kit (TaKaRa Bio Inc.) was used to synthesize the first-strand cDNA from total RNA of mutant ΔTpKu70 as the template, according to the manufacturer’s instruction.

    Techniques: Quantitative RT-PCR, Cell Culture, Expressing, Mutagenesis, Polymerase Chain Reaction

    Expression of retinoblastoma (RB) in vitro and in vivo. (A) Detection of RB94 in A549 cells by western blot analysis. (B) Reverse transcription-quantitative polymerase chain reaction analysis of RB94 mRNA levels in vivo . Data are expressed as the means ± SD (n=3). ** P

    Journal: Molecular Medicine Reports

    Article Title: Role of adenovirus-mediated retinoblastoma 94 in the treatment of human non-small cell lung cancer

    doi: 10.3892/mmr.2015.3227

    Figure Lengend Snippet: Expression of retinoblastoma (RB) in vitro and in vivo. (A) Detection of RB94 in A549 cells by western blot analysis. (B) Reverse transcription-quantitative polymerase chain reaction analysis of RB94 mRNA levels in vivo . Data are expressed as the means ± SD (n=3). ** P

    Article Snippet: Reverse transcription-quantitative PCR (RT-qPCR) Total mRNA was extracted from tumors in each of the studied groups with an mRNA isolation kit (Takara, Dalian, China), and 3 μg total RNA was used for cDNA synthesis by SuperScript II reverse transcriptase (Invitrogen) according to the standard instructions. qPCR was performed in a final volume of 20 μl containing 1 μl each cDNA template, 2 μl each 10 nM primer (RB94 F: 5′-GAA TCT GCT TGT CCT CTT AAT CTT AAT CTT CC-3′ and R: 5′-GAA GAT GGT GAT GGG ATT TC-3′; cyclinB1 F: 5′-CAG TCA GAC CAA AAT ACC TAC TGG GT-3′ and R: 5′-ACA CAA ACC AGC TGC AGC ATC TTC TT-3′), and 10 μl SYBR-Green Master mix.

    Techniques: Expressing, In Vitro, In Vivo, Western Blot, Real-time Polymerase Chain Reaction