reverse transcription quantitative pcr rt qpcr  (Bio-Rad)

 
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    Bio-Rad reverse transcription quantitative pcr rt qpcr
    Expression of OY-TES-1 mRNA in glioma tissue. (A) Representative 12 <t>RT-PCR</t> results of OY-TES-1 expression in glioma (lanes 1–12), positive control testis samples (lane P) and negative controls with no cDNA (lane N). p53 was amplified as the internal control. (B) <t>RT-qPCR</t> analysis of OY-TES-1 mRNA. OY-TES-1 mRNA was elevated in glioma tissues compared with normal adult tissues, with the exception of testis tissue. The levels of OY-TES-1 mRNA in glioma tissues were significantly higher than in normal brain tissues (P=0.0015). (C) Comparison of OY-TES-1 mRNA between WHO grade I–II and III–IV glioma tissues, as examined by RT-qPCR. The level of OY-TES-1 mRNA was significantly higher in high-grade compared with low-grade glioma samples (*P=0.0002 vs. WHO I–II). Results are presented as the mean ± standard deviation. RT-PCR, reverse transcription-polymerase chain reaction; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; p53, tumor protein 53; WHO, World Health Organization; HPRT, hypoxanthine phosphoribosyl transferase.
    Reverse Transcription Quantitative Pcr Rt Qpcr, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 91/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/reverse transcription quantitative pcr rt qpcr/product/Bio-Rad
    Average 91 stars, based on 22 article reviews
    Price from $9.99 to $1999.99
    reverse transcription quantitative pcr rt qpcr - by Bioz Stars, 2020-07
    91/100 stars

    Images

    1) Product Images from "Serum immunoreactivity of cancer/testis antigen OY-TES-1 and its tissues expression in glioma"

    Article Title: Serum immunoreactivity of cancer/testis antigen OY-TES-1 and its tissues expression in glioma

    Journal: Oncology Letters

    doi: 10.3892/ol.2017.5799

    Expression of OY-TES-1 mRNA in glioma tissue. (A) Representative 12 RT-PCR results of OY-TES-1 expression in glioma (lanes 1–12), positive control testis samples (lane P) and negative controls with no cDNA (lane N). p53 was amplified as the internal control. (B) RT-qPCR analysis of OY-TES-1 mRNA. OY-TES-1 mRNA was elevated in glioma tissues compared with normal adult tissues, with the exception of testis tissue. The levels of OY-TES-1 mRNA in glioma tissues were significantly higher than in normal brain tissues (P=0.0015). (C) Comparison of OY-TES-1 mRNA between WHO grade I–II and III–IV glioma tissues, as examined by RT-qPCR. The level of OY-TES-1 mRNA was significantly higher in high-grade compared with low-grade glioma samples (*P=0.0002 vs. WHO I–II). Results are presented as the mean ± standard deviation. RT-PCR, reverse transcription-polymerase chain reaction; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; p53, tumor protein 53; WHO, World Health Organization; HPRT, hypoxanthine phosphoribosyl transferase.
    Figure Legend Snippet: Expression of OY-TES-1 mRNA in glioma tissue. (A) Representative 12 RT-PCR results of OY-TES-1 expression in glioma (lanes 1–12), positive control testis samples (lane P) and negative controls with no cDNA (lane N). p53 was amplified as the internal control. (B) RT-qPCR analysis of OY-TES-1 mRNA. OY-TES-1 mRNA was elevated in glioma tissues compared with normal adult tissues, with the exception of testis tissue. The levels of OY-TES-1 mRNA in glioma tissues were significantly higher than in normal brain tissues (P=0.0015). (C) Comparison of OY-TES-1 mRNA between WHO grade I–II and III–IV glioma tissues, as examined by RT-qPCR. The level of OY-TES-1 mRNA was significantly higher in high-grade compared with low-grade glioma samples (*P=0.0002 vs. WHO I–II). Results are presented as the mean ± standard deviation. RT-PCR, reverse transcription-polymerase chain reaction; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; p53, tumor protein 53; WHO, World Health Organization; HPRT, hypoxanthine phosphoribosyl transferase.

    Techniques Used: Expressing, Reverse Transcription Polymerase Chain Reaction, Positive Control, Amplification, Quantitative RT-PCR, Standard Deviation, Real-time Polymerase Chain Reaction

    2) Product Images from "Two-Step Regulation of a Meristematic Cell Population Acting in Shoot Branching in Arabidopsis"

    Article Title: Two-Step Regulation of a Meristematic Cell Population Acting in Shoot Branching in Arabidopsis

    Journal: PLoS Genetics

    doi: 10.1371/journal.pgen.1006168

    Epigenetic modification of the STM locus. (A) RT-PCR with primers amplifying the REV , STM , or ACT2 coding regions on cDNAs generated from mRNA isolated from Col-0 wild-type inflorescence, stem, mature leaf (without the leaf axil region), root, and petal, respectively. ACT2 was used as a loading control. (B and C) Results of ChIP-qPCR performed on IP with antibodies against H3K27me3 (B) and H3K4me2/3 (C) on chromatin samples extracted from Col-0 wild-type inflorescences and mature leaves. The a-e regions (indicated as in Fig 5B ) were assayed. Error bars indicate SD. More controls are shown in (D). (D) ChIP enrichment test by PCR with an anti-H3K27me3 antibody and an anti-H3K4me2/3 antibody using Col-0 wild-type inflorescences and mature leaves, together with total DNA input (input) and no-antibody (mock) controls. An ACT2 promoter region was used as a negative control. (E) Up-regulation of STM expression in mutants affecting PRC1 and PRC2 requires REV . RT-qPCR analysis of STM in whole seedlings of Col-0 wild type and mutants affecting PRC1 and PRC2 w/ or w/o rev-6 . The vertical axis indicates relative mRNA amount compared with the amount in wild-type plants. Error bars indicate SD.
    Figure Legend Snippet: Epigenetic modification of the STM locus. (A) RT-PCR with primers amplifying the REV , STM , or ACT2 coding regions on cDNAs generated from mRNA isolated from Col-0 wild-type inflorescence, stem, mature leaf (without the leaf axil region), root, and petal, respectively. ACT2 was used as a loading control. (B and C) Results of ChIP-qPCR performed on IP with antibodies against H3K27me3 (B) and H3K4me2/3 (C) on chromatin samples extracted from Col-0 wild-type inflorescences and mature leaves. The a-e regions (indicated as in Fig 5B ) were assayed. Error bars indicate SD. More controls are shown in (D). (D) ChIP enrichment test by PCR with an anti-H3K27me3 antibody and an anti-H3K4me2/3 antibody using Col-0 wild-type inflorescences and mature leaves, together with total DNA input (input) and no-antibody (mock) controls. An ACT2 promoter region was used as a negative control. (E) Up-regulation of STM expression in mutants affecting PRC1 and PRC2 requires REV . RT-qPCR analysis of STM in whole seedlings of Col-0 wild type and mutants affecting PRC1 and PRC2 w/ or w/o rev-6 . The vertical axis indicates relative mRNA amount compared with the amount in wild-type plants. Error bars indicate SD.

    Techniques Used: Modification, Reverse Transcription Polymerase Chain Reaction, Generated, Isolation, Chromatin Immunoprecipitation, Real-time Polymerase Chain Reaction, Polymerase Chain Reaction, Negative Control, Expressing, Quantitative RT-PCR

    Direct up-regulation of STM expression by REV. (A) RT-qPCR analysis of STM expression in pREV :: REV-GR-HA rev-6 vegetative shoot apex tissues (with leaves removed) before and after simultaneous Dex and CHX treatment. The vertical axis indicates relative mRNA amount compared with the amount before treatment. Error bars indicate SD. (B) Schematic diagram of the STM genomic region. Vertical red lines indicate the sites containing the consensus REV binding sequence (ATGAT box). ATG denotes the translation start site. The underlying lines represent the DNA fragments amplified in ChIP assays, or used for plant protoplast assays. (C and D) ChIP enrichment test by PCR shows binding of REV-GR-HA to the ATGAT box-containing regions, especially the ones near the start site, in vegetative shoot apex tissues enriched with leaf axil (C) but not mature leaves ( > P 10 ) without the leaf axil region from 30-d old plants (D) of pREV :: REV-GR-HA rev-6 plants. A paired design was used, in which each measurement was paired with a corresponding control without antibody. Error bars indicate SD. More controls are shown in S4I Fig . (E) Transcriptional activity assays in Arabidopsis protoplasts. A p35S :: GFP empty vector was the negative control, and a p35S :: GUS line was the internal control. Relative LUC reporter gene expression is shown in the lower panel. The p1-p5 regions (indicated as in B) were assayed. Data are mean ± SD. Error bars are derived from three independent biological experiments, each run in triplicate.
    Figure Legend Snippet: Direct up-regulation of STM expression by REV. (A) RT-qPCR analysis of STM expression in pREV :: REV-GR-HA rev-6 vegetative shoot apex tissues (with leaves removed) before and after simultaneous Dex and CHX treatment. The vertical axis indicates relative mRNA amount compared with the amount before treatment. Error bars indicate SD. (B) Schematic diagram of the STM genomic region. Vertical red lines indicate the sites containing the consensus REV binding sequence (ATGAT box). ATG denotes the translation start site. The underlying lines represent the DNA fragments amplified in ChIP assays, or used for plant protoplast assays. (C and D) ChIP enrichment test by PCR shows binding of REV-GR-HA to the ATGAT box-containing regions, especially the ones near the start site, in vegetative shoot apex tissues enriched with leaf axil (C) but not mature leaves ( > P 10 ) without the leaf axil region from 30-d old plants (D) of pREV :: REV-GR-HA rev-6 plants. A paired design was used, in which each measurement was paired with a corresponding control without antibody. Error bars indicate SD. More controls are shown in S4I Fig . (E) Transcriptional activity assays in Arabidopsis protoplasts. A p35S :: GFP empty vector was the negative control, and a p35S :: GUS line was the internal control. Relative LUC reporter gene expression is shown in the lower panel. The p1-p5 regions (indicated as in B) were assayed. Data are mean ± SD. Error bars are derived from three independent biological experiments, each run in triplicate.

    Techniques Used: Expressing, Quantitative RT-PCR, Binding Assay, Sequencing, Amplification, Chromatin Immunoprecipitation, Polymerase Chain Reaction, Activity Assay, Plasmid Preparation, Negative Control, Derivative Assay

    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: Serum immunoreactivity of cancer/testis antigen OY-TES-1 and its tissues expression in glioma
    Article Snippet: .. Reverse transcription quantitative PCR (RT-qPCR) The presence of OY-TES-1 mRNA was quantitatively detected using the iCycler iQ™ Multi-Color Real-Time PCR Detection system (Bio-Rad Laboratories, Inc.) with the following primer sequences: Sense, 5′-GCGACACCTCCCACAAGAC-3′ and antisense, 5′-GCCCACCGTACAAATCCAG-3′. ..

    Article Title: Persistence of subclinical deformed wing virus infections in honeybees following Varroa mite removal and a bee population turnover
    Article Snippet: .. RT-qPCR The amount of deformed wing virus (DWV) RNA and RP49 mRNA (an internal reference gene for normalizing between-sample differences in RNA quantity and quality) in the adult and pupal bee samples was determined using reverse transcription quantitative PCR (RT-qPCR), using the iScript One Step RT-PCR kit (Bio-Rad) with SYBR Green as the detection chemistry and the Bio-Rad CFX connect thermocycler. .. Before RT-qPCR the RNA samples were diluted to a uniform concentration of 20 ng/μL to avoid concentration-dependent effects on RT-qPCR efficiency [ ].

    Article Title: Genome-Wide Identification and Characterization of NODULE-INCEPTION-Like Protein (NLP) Family Genes in Brassica napus
    Article Snippet: .. Reverse transcription quantitative PCR (RT-qPCR) was performed on a Bio-Rad CFX96 Real Time System (USA) according to a previously described method [ ]. ..

    Article Title: Two-Step Regulation of a Meristematic Cell Population Acting in Shoot Branching in Arabidopsis
    Article Snippet: .. Reverse transcription quantitative PCR (RT-qPCR) was performed on a Bio-Rad CFX96 real-time PCR detection system with the KAPA SYBR FAST qPCR kit (KAPA Biosystems). .. Relative expression by RT-qPCR was normalized to TUB6 (At5g12250 ).

    Article Title: Dark/Light Treatments Followed by γ-Irradiation Increase the Frequency of Leaf-Color Mutants in Cymbidium
    Article Snippet: .. Reverse transcription quantitative PCR (RT-qPCR) was conducted with iQ SYBR Green Supermix (Bio-Rad, Hercules, CA, USA) using the CFX96 Touch Real-Time PCR Detection System (Bio-Rad, Hercules, CA, USA). ..

    Article Title: A Protease-Independent Function for SPPL3 in NFAT Activation
    Article Snippet: .. Reverse transcription-quantitative PCR (RT-qPCR) was performed on a CFX Connect system (Bio-Rad) using Maxima Probe/ROX qPCR master mix (Fermentas) and TaqMan gene expression assay sets (Applied Biosystems) for human glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (catalog no. 4352934), SPPL3 (catalog no. 00293370 and 01000610), IL-2 (catalog no. 00174114), TNF-α (catalog no. 01113624), gamma interferon (IFN-γ) (catalog no. 00989291), granulocyte-macrophage colony-stimulating factor (GM-CSF) (catalog no. 00929873), and FasL (catalog no. 00181225), and results were analyzed using the standard-curve method. .. HEK293T cells plated at 2 × 105 /dish in 35-mm glass-bottom petri dishes with 14-mm microwells (catalog no. P35G-0-14-C; MatTek) were TransIT-LT1 transfected 24 h later with 1 μg total DNA, including 500 ng D1ER and 200 ng mCherry-myc-SPPL3.

    Quantitative RT-PCR:

    Article Title: Serum immunoreactivity of cancer/testis antigen OY-TES-1 and its tissues expression in glioma
    Article Snippet: .. Reverse transcription quantitative PCR (RT-qPCR) The presence of OY-TES-1 mRNA was quantitatively detected using the iCycler iQ™ Multi-Color Real-Time PCR Detection system (Bio-Rad Laboratories, Inc.) with the following primer sequences: Sense, 5′-GCGACACCTCCCACAAGAC-3′ and antisense, 5′-GCCCACCGTACAAATCCAG-3′. ..

    Article Title: Persistence of subclinical deformed wing virus infections in honeybees following Varroa mite removal and a bee population turnover
    Article Snippet: .. RT-qPCR The amount of deformed wing virus (DWV) RNA and RP49 mRNA (an internal reference gene for normalizing between-sample differences in RNA quantity and quality) in the adult and pupal bee samples was determined using reverse transcription quantitative PCR (RT-qPCR), using the iScript One Step RT-PCR kit (Bio-Rad) with SYBR Green as the detection chemistry and the Bio-Rad CFX connect thermocycler. .. Before RT-qPCR the RNA samples were diluted to a uniform concentration of 20 ng/μL to avoid concentration-dependent effects on RT-qPCR efficiency [ ].

    Article Title: Genome-Wide Identification and Characterization of NODULE-INCEPTION-Like Protein (NLP) Family Genes in Brassica napus
    Article Snippet: .. Reverse transcription quantitative PCR (RT-qPCR) was performed on a Bio-Rad CFX96 Real Time System (USA) according to a previously described method [ ]. ..

    Article Title: Role of RNA Secondary Structure and Processing in Stability of the nifH1 Transcript in the Cyanobacterium Anabaena variabilis
    Article Snippet: .. Reverse transcription-quantitative PCR (RT-qPCR) was used to determine the quantities and decay rates of transcripts. cDNA was made from 40 ng of RNA using Bio-Rad iScript Reverse Transcription Super Mix (catalog no. 170-8841) in a 10-μl reaction mixture. .. The cDNA was diluted 1:20 to 0.2 ng μl−1 for qPCR. qPCRs were done using Bio-Rad SsoAdvanced SYBR Green SuperMix (catalog no. 1725261) in a 10-μl reaction volume and contained 0.8 ng cDNA, 5 pmol of gene-specific primers, and 1× reaction supermix.

    Article Title: Two-Step Regulation of a Meristematic Cell Population Acting in Shoot Branching in Arabidopsis
    Article Snippet: .. Reverse transcription quantitative PCR (RT-qPCR) was performed on a Bio-Rad CFX96 real-time PCR detection system with the KAPA SYBR FAST qPCR kit (KAPA Biosystems). .. Relative expression by RT-qPCR was normalized to TUB6 (At5g12250 ).

    Article Title: Infectious Bronchitis Coronavirus Inhibits STAT1 Signaling and Requires Accessory Proteins for Resistance to Type I Interferon Activity
    Article Snippet: .. Reverse transcription-quantitative PCR (RT-qPCR) was performed on 5 μl of RNA using the SYBR green one-step kit (Bio-Rad) in a Bio-Rad CFX96 PCR apparatus. .. Primers against the nucleocapsid gene of IBV, based on the sequence with GenBank accession number , were as previously published ( ).

    Article Title: Dark/Light Treatments Followed by γ-Irradiation Increase the Frequency of Leaf-Color Mutants in Cymbidium
    Article Snippet: .. Reverse transcription quantitative PCR (RT-qPCR) was conducted with iQ SYBR Green Supermix (Bio-Rad, Hercules, CA, USA) using the CFX96 Touch Real-Time PCR Detection System (Bio-Rad, Hercules, CA, USA). ..

    Article Title: A Protease-Independent Function for SPPL3 in NFAT Activation
    Article Snippet: .. Reverse transcription-quantitative PCR (RT-qPCR) was performed on a CFX Connect system (Bio-Rad) using Maxima Probe/ROX qPCR master mix (Fermentas) and TaqMan gene expression assay sets (Applied Biosystems) for human glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (catalog no. 4352934), SPPL3 (catalog no. 00293370 and 01000610), IL-2 (catalog no. 00174114), TNF-α (catalog no. 01113624), gamma interferon (IFN-γ) (catalog no. 00989291), granulocyte-macrophage colony-stimulating factor (GM-CSF) (catalog no. 00929873), and FasL (catalog no. 00181225), and results were analyzed using the standard-curve method. .. HEK293T cells plated at 2 × 105 /dish in 35-mm glass-bottom petri dishes with 14-mm microwells (catalog no. P35G-0-14-C; MatTek) were TransIT-LT1 transfected 24 h later with 1 μg total DNA, including 500 ng D1ER and 200 ng mCherry-myc-SPPL3.

    SYBR Green Assay:

    Article Title: Persistence of subclinical deformed wing virus infections in honeybees following Varroa mite removal and a bee population turnover
    Article Snippet: .. RT-qPCR The amount of deformed wing virus (DWV) RNA and RP49 mRNA (an internal reference gene for normalizing between-sample differences in RNA quantity and quality) in the adult and pupal bee samples was determined using reverse transcription quantitative PCR (RT-qPCR), using the iScript One Step RT-PCR kit (Bio-Rad) with SYBR Green as the detection chemistry and the Bio-Rad CFX connect thermocycler. .. Before RT-qPCR the RNA samples were diluted to a uniform concentration of 20 ng/μL to avoid concentration-dependent effects on RT-qPCR efficiency [ ].

    Article Title: Infectious Bronchitis Coronavirus Inhibits STAT1 Signaling and Requires Accessory Proteins for Resistance to Type I Interferon Activity
    Article Snippet: .. Reverse transcription-quantitative PCR (RT-qPCR) was performed on 5 μl of RNA using the SYBR green one-step kit (Bio-Rad) in a Bio-Rad CFX96 PCR apparatus. .. Primers against the nucleocapsid gene of IBV, based on the sequence with GenBank accession number , were as previously published ( ).

    Article Title: Dark/Light Treatments Followed by γ-Irradiation Increase the Frequency of Leaf-Color Mutants in Cymbidium
    Article Snippet: .. Reverse transcription quantitative PCR (RT-qPCR) was conducted with iQ SYBR Green Supermix (Bio-Rad, Hercules, CA, USA) using the CFX96 Touch Real-Time PCR Detection System (Bio-Rad, Hercules, CA, USA). ..

    Polymerase Chain Reaction:

    Article Title: Role of RNA Secondary Structure and Processing in Stability of the nifH1 Transcript in the Cyanobacterium Anabaena variabilis
    Article Snippet: .. Reverse transcription-quantitative PCR (RT-qPCR) was used to determine the quantities and decay rates of transcripts. cDNA was made from 40 ng of RNA using Bio-Rad iScript Reverse Transcription Super Mix (catalog no. 170-8841) in a 10-μl reaction mixture. .. The cDNA was diluted 1:20 to 0.2 ng μl−1 for qPCR. qPCRs were done using Bio-Rad SsoAdvanced SYBR Green SuperMix (catalog no. 1725261) in a 10-μl reaction volume and contained 0.8 ng cDNA, 5 pmol of gene-specific primers, and 1× reaction supermix.

    Article Title: Infectious Bronchitis Coronavirus Inhibits STAT1 Signaling and Requires Accessory Proteins for Resistance to Type I Interferon Activity
    Article Snippet: .. Reverse transcription-quantitative PCR (RT-qPCR) was performed on 5 μl of RNA using the SYBR green one-step kit (Bio-Rad) in a Bio-Rad CFX96 PCR apparatus. .. Primers against the nucleocapsid gene of IBV, based on the sequence with GenBank accession number , were as previously published ( ).

    Article Title: A Protease-Independent Function for SPPL3 in NFAT Activation
    Article Snippet: .. Reverse transcription-quantitative PCR (RT-qPCR) was performed on a CFX Connect system (Bio-Rad) using Maxima Probe/ROX qPCR master mix (Fermentas) and TaqMan gene expression assay sets (Applied Biosystems) for human glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (catalog no. 4352934), SPPL3 (catalog no. 00293370 and 01000610), IL-2 (catalog no. 00174114), TNF-α (catalog no. 01113624), gamma interferon (IFN-γ) (catalog no. 00989291), granulocyte-macrophage colony-stimulating factor (GM-CSF) (catalog no. 00929873), and FasL (catalog no. 00181225), and results were analyzed using the standard-curve method. .. HEK293T cells plated at 2 × 105 /dish in 35-mm glass-bottom petri dishes with 14-mm microwells (catalog no. P35G-0-14-C; MatTek) were TransIT-LT1 transfected 24 h later with 1 μg total DNA, including 500 ng D1ER and 200 ng mCherry-myc-SPPL3.

    Expressing:

    Article Title: A Protease-Independent Function for SPPL3 in NFAT Activation
    Article Snippet: .. Reverse transcription-quantitative PCR (RT-qPCR) was performed on a CFX Connect system (Bio-Rad) using Maxima Probe/ROX qPCR master mix (Fermentas) and TaqMan gene expression assay sets (Applied Biosystems) for human glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (catalog no. 4352934), SPPL3 (catalog no. 00293370 and 01000610), IL-2 (catalog no. 00174114), TNF-α (catalog no. 01113624), gamma interferon (IFN-γ) (catalog no. 00989291), granulocyte-macrophage colony-stimulating factor (GM-CSF) (catalog no. 00929873), and FasL (catalog no. 00181225), and results were analyzed using the standard-curve method. .. HEK293T cells plated at 2 × 105 /dish in 35-mm glass-bottom petri dishes with 14-mm microwells (catalog no. P35G-0-14-C; MatTek) were TransIT-LT1 transfected 24 h later with 1 μg total DNA, including 500 ng D1ER and 200 ng mCherry-myc-SPPL3.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Persistence of subclinical deformed wing virus infections in honeybees following Varroa mite removal and a bee population turnover
    Article Snippet: .. RT-qPCR The amount of deformed wing virus (DWV) RNA and RP49 mRNA (an internal reference gene for normalizing between-sample differences in RNA quantity and quality) in the adult and pupal bee samples was determined using reverse transcription quantitative PCR (RT-qPCR), using the iScript One Step RT-PCR kit (Bio-Rad) with SYBR Green as the detection chemistry and the Bio-Rad CFX connect thermocycler. .. Before RT-qPCR the RNA samples were diluted to a uniform concentration of 20 ng/μL to avoid concentration-dependent effects on RT-qPCR efficiency [ ].

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    Bio-Rad reverse transcription quantitative pcr rt qpcr
    Expression of OY-TES-1 mRNA in glioma tissue. (A) Representative 12 <t>RT-PCR</t> results of OY-TES-1 expression in glioma (lanes 1–12), positive control testis samples (lane P) and negative controls with no cDNA (lane N). p53 was amplified as the internal control. (B) <t>RT-qPCR</t> analysis of OY-TES-1 mRNA. OY-TES-1 mRNA was elevated in glioma tissues compared with normal adult tissues, with the exception of testis tissue. The levels of OY-TES-1 mRNA in glioma tissues were significantly higher than in normal brain tissues (P=0.0015). (C) Comparison of OY-TES-1 mRNA between WHO grade I–II and III–IV glioma tissues, as examined by RT-qPCR. The level of OY-TES-1 mRNA was significantly higher in high-grade compared with low-grade glioma samples (*P=0.0002 vs. WHO I–II). Results are presented as the mean ± standard deviation. RT-PCR, reverse transcription-polymerase chain reaction; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; p53, tumor protein 53; WHO, World Health Organization; HPRT, hypoxanthine phosphoribosyl transferase.
    Reverse Transcription Quantitative Pcr Rt Qpcr, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 91/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/reverse transcription quantitative pcr rt qpcr/product/Bio-Rad
    Average 91 stars, based on 29 article reviews
    Price from $9.99 to $1999.99
    reverse transcription quantitative pcr rt qpcr - by Bioz Stars, 2020-07
    91/100 stars
      Buy from Supplier

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    Expression of OY-TES-1 mRNA in glioma tissue. (A) Representative 12 RT-PCR results of OY-TES-1 expression in glioma (lanes 1–12), positive control testis samples (lane P) and negative controls with no cDNA (lane N). p53 was amplified as the internal control. (B) RT-qPCR analysis of OY-TES-1 mRNA. OY-TES-1 mRNA was elevated in glioma tissues compared with normal adult tissues, with the exception of testis tissue. The levels of OY-TES-1 mRNA in glioma tissues were significantly higher than in normal brain tissues (P=0.0015). (C) Comparison of OY-TES-1 mRNA between WHO grade I–II and III–IV glioma tissues, as examined by RT-qPCR. The level of OY-TES-1 mRNA was significantly higher in high-grade compared with low-grade glioma samples (*P=0.0002 vs. WHO I–II). Results are presented as the mean ± standard deviation. RT-PCR, reverse transcription-polymerase chain reaction; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; p53, tumor protein 53; WHO, World Health Organization; HPRT, hypoxanthine phosphoribosyl transferase.

    Journal: Oncology Letters

    Article Title: Serum immunoreactivity of cancer/testis antigen OY-TES-1 and its tissues expression in glioma

    doi: 10.3892/ol.2017.5799

    Figure Lengend Snippet: Expression of OY-TES-1 mRNA in glioma tissue. (A) Representative 12 RT-PCR results of OY-TES-1 expression in glioma (lanes 1–12), positive control testis samples (lane P) and negative controls with no cDNA (lane N). p53 was amplified as the internal control. (B) RT-qPCR analysis of OY-TES-1 mRNA. OY-TES-1 mRNA was elevated in glioma tissues compared with normal adult tissues, with the exception of testis tissue. The levels of OY-TES-1 mRNA in glioma tissues were significantly higher than in normal brain tissues (P=0.0015). (C) Comparison of OY-TES-1 mRNA between WHO grade I–II and III–IV glioma tissues, as examined by RT-qPCR. The level of OY-TES-1 mRNA was significantly higher in high-grade compared with low-grade glioma samples (*P=0.0002 vs. WHO I–II). Results are presented as the mean ± standard deviation. RT-PCR, reverse transcription-polymerase chain reaction; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; p53, tumor protein 53; WHO, World Health Organization; HPRT, hypoxanthine phosphoribosyl transferase.

    Article Snippet: Reverse transcription quantitative PCR (RT-qPCR) The presence of OY-TES-1 mRNA was quantitatively detected using the iCycler iQ™ Multi-Color Real-Time PCR Detection system (Bio-Rad Laboratories, Inc.) with the following primer sequences: Sense, 5′-GCGACACCTCCCACAAGAC-3′ and antisense, 5′-GCCCACCGTACAAATCCAG-3′.

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Positive Control, Amplification, Quantitative RT-PCR, Standard Deviation, Real-time Polymerase Chain Reaction

    Epigenetic modification of the STM locus. (A) RT-PCR with primers amplifying the REV , STM , or ACT2 coding regions on cDNAs generated from mRNA isolated from Col-0 wild-type inflorescence, stem, mature leaf (without the leaf axil region), root, and petal, respectively. ACT2 was used as a loading control. (B and C) Results of ChIP-qPCR performed on IP with antibodies against H3K27me3 (B) and H3K4me2/3 (C) on chromatin samples extracted from Col-0 wild-type inflorescences and mature leaves. The a-e regions (indicated as in Fig 5B ) were assayed. Error bars indicate SD. More controls are shown in (D). (D) ChIP enrichment test by PCR with an anti-H3K27me3 antibody and an anti-H3K4me2/3 antibody using Col-0 wild-type inflorescences and mature leaves, together with total DNA input (input) and no-antibody (mock) controls. An ACT2 promoter region was used as a negative control. (E) Up-regulation of STM expression in mutants affecting PRC1 and PRC2 requires REV . RT-qPCR analysis of STM in whole seedlings of Col-0 wild type and mutants affecting PRC1 and PRC2 w/ or w/o rev-6 . The vertical axis indicates relative mRNA amount compared with the amount in wild-type plants. Error bars indicate SD.

    Journal: PLoS Genetics

    Article Title: Two-Step Regulation of a Meristematic Cell Population Acting in Shoot Branching in Arabidopsis

    doi: 10.1371/journal.pgen.1006168

    Figure Lengend Snippet: Epigenetic modification of the STM locus. (A) RT-PCR with primers amplifying the REV , STM , or ACT2 coding regions on cDNAs generated from mRNA isolated from Col-0 wild-type inflorescence, stem, mature leaf (without the leaf axil region), root, and petal, respectively. ACT2 was used as a loading control. (B and C) Results of ChIP-qPCR performed on IP with antibodies against H3K27me3 (B) and H3K4me2/3 (C) on chromatin samples extracted from Col-0 wild-type inflorescences and mature leaves. The a-e regions (indicated as in Fig 5B ) were assayed. Error bars indicate SD. More controls are shown in (D). (D) ChIP enrichment test by PCR with an anti-H3K27me3 antibody and an anti-H3K4me2/3 antibody using Col-0 wild-type inflorescences and mature leaves, together with total DNA input (input) and no-antibody (mock) controls. An ACT2 promoter region was used as a negative control. (E) Up-regulation of STM expression in mutants affecting PRC1 and PRC2 requires REV . RT-qPCR analysis of STM in whole seedlings of Col-0 wild type and mutants affecting PRC1 and PRC2 w/ or w/o rev-6 . The vertical axis indicates relative mRNA amount compared with the amount in wild-type plants. Error bars indicate SD.

    Article Snippet: Reverse transcription quantitative PCR (RT-qPCR) was performed on a Bio-Rad CFX96 real-time PCR detection system with the KAPA SYBR FAST qPCR kit (KAPA Biosystems).

    Techniques: Modification, Reverse Transcription Polymerase Chain Reaction, Generated, Isolation, Chromatin Immunoprecipitation, Real-time Polymerase Chain Reaction, Polymerase Chain Reaction, Negative Control, Expressing, Quantitative RT-PCR

    Direct up-regulation of STM expression by REV. (A) RT-qPCR analysis of STM expression in pREV :: REV-GR-HA rev-6 vegetative shoot apex tissues (with leaves removed) before and after simultaneous Dex and CHX treatment. The vertical axis indicates relative mRNA amount compared with the amount before treatment. Error bars indicate SD. (B) Schematic diagram of the STM genomic region. Vertical red lines indicate the sites containing the consensus REV binding sequence (ATGAT box). ATG denotes the translation start site. The underlying lines represent the DNA fragments amplified in ChIP assays, or used for plant protoplast assays. (C and D) ChIP enrichment test by PCR shows binding of REV-GR-HA to the ATGAT box-containing regions, especially the ones near the start site, in vegetative shoot apex tissues enriched with leaf axil (C) but not mature leaves ( > P 10 ) without the leaf axil region from 30-d old plants (D) of pREV :: REV-GR-HA rev-6 plants. A paired design was used, in which each measurement was paired with a corresponding control without antibody. Error bars indicate SD. More controls are shown in S4I Fig . (E) Transcriptional activity assays in Arabidopsis protoplasts. A p35S :: GFP empty vector was the negative control, and a p35S :: GUS line was the internal control. Relative LUC reporter gene expression is shown in the lower panel. The p1-p5 regions (indicated as in B) were assayed. Data are mean ± SD. Error bars are derived from three independent biological experiments, each run in triplicate.

    Journal: PLoS Genetics

    Article Title: Two-Step Regulation of a Meristematic Cell Population Acting in Shoot Branching in Arabidopsis

    doi: 10.1371/journal.pgen.1006168

    Figure Lengend Snippet: Direct up-regulation of STM expression by REV. (A) RT-qPCR analysis of STM expression in pREV :: REV-GR-HA rev-6 vegetative shoot apex tissues (with leaves removed) before and after simultaneous Dex and CHX treatment. The vertical axis indicates relative mRNA amount compared with the amount before treatment. Error bars indicate SD. (B) Schematic diagram of the STM genomic region. Vertical red lines indicate the sites containing the consensus REV binding sequence (ATGAT box). ATG denotes the translation start site. The underlying lines represent the DNA fragments amplified in ChIP assays, or used for plant protoplast assays. (C and D) ChIP enrichment test by PCR shows binding of REV-GR-HA to the ATGAT box-containing regions, especially the ones near the start site, in vegetative shoot apex tissues enriched with leaf axil (C) but not mature leaves ( > P 10 ) without the leaf axil region from 30-d old plants (D) of pREV :: REV-GR-HA rev-6 plants. A paired design was used, in which each measurement was paired with a corresponding control without antibody. Error bars indicate SD. More controls are shown in S4I Fig . (E) Transcriptional activity assays in Arabidopsis protoplasts. A p35S :: GFP empty vector was the negative control, and a p35S :: GUS line was the internal control. Relative LUC reporter gene expression is shown in the lower panel. The p1-p5 regions (indicated as in B) were assayed. Data are mean ± SD. Error bars are derived from three independent biological experiments, each run in triplicate.

    Article Snippet: Reverse transcription quantitative PCR (RT-qPCR) was performed on a Bio-Rad CFX96 real-time PCR detection system with the KAPA SYBR FAST qPCR kit (KAPA Biosystems).

    Techniques: Expressing, Quantitative RT-PCR, Binding Assay, Sequencing, Amplification, Chromatin Immunoprecipitation, Polymerase Chain Reaction, Activity Assay, Plasmid Preparation, Negative Control, Derivative Assay