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    TaKaRa reverse transcription quantitative pcr rt qpcr analysis
    Expression profiles of members of the CPF/CPFL gene families as determined by <t>RT-PCR</t> and <t>RT-qPCR.</t> ( A ) Expression of CPF/CPFL gene-annotated nymph cuticle protein genes in different tissues on day 6 of 5 th instar nymphs as detected by RT-PCR (28 cycles). Different tissues are listed: Integument, head, leg, pronotum, goad, gut, Malpighian tubules, fat body, wing pad; ( B ) Expression of CPF/CPFL gene-annotated adult cuticle protein genes in different tissues on day 2 of adults, as detected by RT-PCR (28 cycles). Different tissues are listed: Integument, leg, muscle, tentacle, goad, gut, Malpighian tubules, fat body, wing. β-actin was used as the reference control (24 cycles). The image were obtained by using the gel imaging analysis system (Bio-Rad, USA). The full-length gels are presented in Supplementary Fig. 6 . ( C ) Expression of CPF/CPFL genes in the pronotum after molt from 4 th instar nymphs to 5 th instar nymphs at 0 h, 36 h, 72 h and 96 h, as detected by RT-qPCR. β-actin was used as the reference control. Data are reported as means ± SE of three independent biological replications.
    Reverse Transcription Quantitative Pcr Rt Qpcr Analysis, supplied by TaKaRa, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/reverse transcription quantitative pcr rt qpcr analysis/product/TaKaRa
    Average 91 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    reverse transcription quantitative pcr rt qpcr analysis - by Bioz Stars, 2020-07
    91/100 stars

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    1) Product Images from "Identification and expression of cuticular protein genes based on Locusta migratoria transcriptome"

    Article Title: Identification and expression of cuticular protein genes based on Locusta migratoria transcriptome

    Journal: Scientific Reports

    doi: 10.1038/srep45462

    Expression profiles of members of the CPF/CPFL gene families as determined by RT-PCR and RT-qPCR. ( A ) Expression of CPF/CPFL gene-annotated nymph cuticle protein genes in different tissues on day 6 of 5 th instar nymphs as detected by RT-PCR (28 cycles). Different tissues are listed: Integument, head, leg, pronotum, goad, gut, Malpighian tubules, fat body, wing pad; ( B ) Expression of CPF/CPFL gene-annotated adult cuticle protein genes in different tissues on day 2 of adults, as detected by RT-PCR (28 cycles). Different tissues are listed: Integument, leg, muscle, tentacle, goad, gut, Malpighian tubules, fat body, wing. β-actin was used as the reference control (24 cycles). The image were obtained by using the gel imaging analysis system (Bio-Rad, USA). The full-length gels are presented in Supplementary Fig. 6 . ( C ) Expression of CPF/CPFL genes in the pronotum after molt from 4 th instar nymphs to 5 th instar nymphs at 0 h, 36 h, 72 h and 96 h, as detected by RT-qPCR. β-actin was used as the reference control. Data are reported as means ± SE of three independent biological replications.
    Figure Legend Snippet: Expression profiles of members of the CPF/CPFL gene families as determined by RT-PCR and RT-qPCR. ( A ) Expression of CPF/CPFL gene-annotated nymph cuticle protein genes in different tissues on day 6 of 5 th instar nymphs as detected by RT-PCR (28 cycles). Different tissues are listed: Integument, head, leg, pronotum, goad, gut, Malpighian tubules, fat body, wing pad; ( B ) Expression of CPF/CPFL gene-annotated adult cuticle protein genes in different tissues on day 2 of adults, as detected by RT-PCR (28 cycles). Different tissues are listed: Integument, leg, muscle, tentacle, goad, gut, Malpighian tubules, fat body, wing. β-actin was used as the reference control (24 cycles). The image were obtained by using the gel imaging analysis system (Bio-Rad, USA). The full-length gels are presented in Supplementary Fig. 6 . ( C ) Expression of CPF/CPFL genes in the pronotum after molt from 4 th instar nymphs to 5 th instar nymphs at 0 h, 36 h, 72 h and 96 h, as detected by RT-qPCR. β-actin was used as the reference control. Data are reported as means ± SE of three independent biological replications.

    Techniques Used: Expressing, Reverse Transcription Polymerase Chain Reaction, Quantitative RT-PCR, Imaging

    Expression profiles of the members of the CPR gene families (RR1, RR2 and RR3) as determined by RT-PCR and RT-qPCR. ( A ) Expression of RR-1 gene-annotated endocuticle protein genes in different tissues on day 6 of 5 th instar nymphs, as detected by RT-PCR (28 cycles). Different tissues: Integument, head, leg, pronotum, goad, gut, Malpighian tubules, fat body, wing pad; ( B ) Expression of RR-1 genes in the pronotum after molting from 4 th instar nymphs to 5 th instar nymphs at 0 h, 36 h, 72 h and 96 h, as detected by RT-qPCR. Heat map showing the relative expression level during different stages of RR-1 genes. The colors in the map display the relative values of all tiles within the 4 given developmental stages. Blue indicates the lowest expression, green indicates intermediate expression, and red indicates the highest expression. The color scale bar is shown on the right of the figure. ( C ) Expression of RR-2 gene-annotated adult cuticle protein genes in different tissues on day 2 of adults as detected by RT-PCR (28 cycles). Different tissues: Integument, leg, muscle, tentacle, goad, gut, Malpighian tubules, fat body, wing. ( D ) Expression of RR-2 genes in whole body, wing pad or wing from embryo, 1, 2 nd , 3 rd , 4 th , 5 th instar nymphs to the adults as detected by RT-qPCR. ( E ) Expression of RR-2 genes in the wing of 5 th instar nymphs at different days as detected by RT-qPCR. N4D1, N4D3, N4D5: Day 1, Day 3, Day 5 of 5 th instar nymphs. ( F ) Expression of RR-3 genes in different tissues on day 6 of 5 th instar nymphs as detected by RT-PCR (28 cycles). Different tissues: Integument, head, leg, goad, gut, Malpighian tubules, fat body, wing pad, pronotum; ( G ) Expression of RR-3 genes in the pronotum after molting from 4 th instar nymphs to 5 th instar nymphs at 0 h, 36 h, 72 h and 96 h, as detected by RT-qPCR. β-actin was used as the reference control for RT-PCR (24 cycles) and RT-qPCR, respectively. All data are reported as means ± SE of three independent biological replications. The electrophoresis image were obtained by using the gel imaging analysis system (Bio-Rad, USA). The full-length gels are presented in Supplementary Figs 1–4 , Figs 2–4 and Figs 3–4 , respectively.
    Figure Legend Snippet: Expression profiles of the members of the CPR gene families (RR1, RR2 and RR3) as determined by RT-PCR and RT-qPCR. ( A ) Expression of RR-1 gene-annotated endocuticle protein genes in different tissues on day 6 of 5 th instar nymphs, as detected by RT-PCR (28 cycles). Different tissues: Integument, head, leg, pronotum, goad, gut, Malpighian tubules, fat body, wing pad; ( B ) Expression of RR-1 genes in the pronotum after molting from 4 th instar nymphs to 5 th instar nymphs at 0 h, 36 h, 72 h and 96 h, as detected by RT-qPCR. Heat map showing the relative expression level during different stages of RR-1 genes. The colors in the map display the relative values of all tiles within the 4 given developmental stages. Blue indicates the lowest expression, green indicates intermediate expression, and red indicates the highest expression. The color scale bar is shown on the right of the figure. ( C ) Expression of RR-2 gene-annotated adult cuticle protein genes in different tissues on day 2 of adults as detected by RT-PCR (28 cycles). Different tissues: Integument, leg, muscle, tentacle, goad, gut, Malpighian tubules, fat body, wing. ( D ) Expression of RR-2 genes in whole body, wing pad or wing from embryo, 1, 2 nd , 3 rd , 4 th , 5 th instar nymphs to the adults as detected by RT-qPCR. ( E ) Expression of RR-2 genes in the wing of 5 th instar nymphs at different days as detected by RT-qPCR. N4D1, N4D3, N4D5: Day 1, Day 3, Day 5 of 5 th instar nymphs. ( F ) Expression of RR-3 genes in different tissues on day 6 of 5 th instar nymphs as detected by RT-PCR (28 cycles). Different tissues: Integument, head, leg, goad, gut, Malpighian tubules, fat body, wing pad, pronotum; ( G ) Expression of RR-3 genes in the pronotum after molting from 4 th instar nymphs to 5 th instar nymphs at 0 h, 36 h, 72 h and 96 h, as detected by RT-qPCR. β-actin was used as the reference control for RT-PCR (24 cycles) and RT-qPCR, respectively. All data are reported as means ± SE of three independent biological replications. The electrophoresis image were obtained by using the gel imaging analysis system (Bio-Rad, USA). The full-length gels are presented in Supplementary Figs 1–4 , Figs 2–4 and Figs 3–4 , respectively.

    Techniques Used: Expressing, Reverse Transcription Polymerase Chain Reaction, Quantitative RT-PCR, Electrophoresis, Imaging

    Neighbor-joining tree and Expression profiles of members of the Tweedle gene families. ( A ) A phylogenetic tree was constructed with the neighbor-joining method of MEGA 5 using the pairwise deletion of indels. Bootstrap support is based on 1000 resembled data sets. GenBank accession numbers are listed in Table S1 . ( B ) Expression of Tweedle genes in different tissues on day 6 of 5 th instar nymphs as detected by RT-PCR (28 cycles). β-actin was used as the reference control (24 cycles). Different tissues are listed: Integument, head, leg, goad, gut, Malpighian tubules, fat body, wing pad, pronotum; The image were obtained by using the gel imaging analysis system (Bio-Rad, USA). The full-length gels are presented in Supplementary Fig. 5 . ( C ) Expression of Tweedle genes in the pronotum of 5 th instar nymphs at different days as detected by RT-qPCR. N5D1, N5D3, N5D5, N5D7: Day 1, Day 3, Day 5, day 7 of 5 th instar nymphs. β-actin was used as the reference control. Data are reported as means ± SE of three independent biological replications.
    Figure Legend Snippet: Neighbor-joining tree and Expression profiles of members of the Tweedle gene families. ( A ) A phylogenetic tree was constructed with the neighbor-joining method of MEGA 5 using the pairwise deletion of indels. Bootstrap support is based on 1000 resembled data sets. GenBank accession numbers are listed in Table S1 . ( B ) Expression of Tweedle genes in different tissues on day 6 of 5 th instar nymphs as detected by RT-PCR (28 cycles). β-actin was used as the reference control (24 cycles). Different tissues are listed: Integument, head, leg, goad, gut, Malpighian tubules, fat body, wing pad, pronotum; The image were obtained by using the gel imaging analysis system (Bio-Rad, USA). The full-length gels are presented in Supplementary Fig. 5 . ( C ) Expression of Tweedle genes in the pronotum of 5 th instar nymphs at different days as detected by RT-qPCR. N5D1, N5D3, N5D5, N5D7: Day 1, Day 3, Day 5, day 7 of 5 th instar nymphs. β-actin was used as the reference control. Data are reported as means ± SE of three independent biological replications.

    Techniques Used: Expressing, Construct, Reverse Transcription Polymerase Chain Reaction, Imaging, Quantitative RT-PCR

    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: Identification and expression of cuticular protein genes based on Locusta migratoria transcriptome
    Article Snippet: .. For reverse-transcription quantitative PCR (RT-qPCR) analysis and SYBR Green kits were used according to the manufacturer’s instructions (TaKaRa, Japan) with specific primers for each gene designed and listed in . .. The total volume of RT-qPCR reactions was 20 μl, containing 10 μl of 2 × SYBR® Premix EX Taq™ (TaKaRa, Japan), 0.4 μl of 50 × ROX Reference Dye (TaKaRa, Japan) and 0.4 μl of specific primers (10 μM), with the following conditions: denaturation at 95 °C for 1 min, followed by 40 cycles at 95 °C for 15 s, 60 °C for 31 s with an ABI 7300 real-time PCR machine (Applied Biosystems, USA) using FastStart Universal SYBR Green Master.

    Article Title: Effect of astragalosides on long non-coding RNA expression profiles in rats with adjuvant-induced arthritis
    Article Snippet: .. Reverse transcription-quantitative PCR (RT-qPCR) analysis The total RNA was extracted using TRIzol reagent and then cDNA was synthesized with PrimeScript™ RT reagent kit (Takara Bio, Inc.) at 65°C for 5 min and 42°C for 60 min. RT-qPCR analysis was performed using a LightCycler 480 II (Roche, Germany) with TB Green™ Advantage® qPCR Premix (Takara Bio, Inc.). .. The thermocycling conditions were as follows: Pre-denaturation at 95°C for 5 min, 40 cycles of 95°C for 15 sec and 60°C for 60 sec.

    SYBR Green Assay:

    Article Title: Identification and expression of cuticular protein genes based on Locusta migratoria transcriptome
    Article Snippet: .. For reverse-transcription quantitative PCR (RT-qPCR) analysis and SYBR Green kits were used according to the manufacturer’s instructions (TaKaRa, Japan) with specific primers for each gene designed and listed in . .. The total volume of RT-qPCR reactions was 20 μl, containing 10 μl of 2 × SYBR® Premix EX Taq™ (TaKaRa, Japan), 0.4 μl of 50 × ROX Reference Dye (TaKaRa, Japan) and 0.4 μl of specific primers (10 μM), with the following conditions: denaturation at 95 °C for 1 min, followed by 40 cycles at 95 °C for 15 s, 60 °C for 31 s with an ABI 7300 real-time PCR machine (Applied Biosystems, USA) using FastStart Universal SYBR Green Master.

    Polymerase Chain Reaction:

    Article Title: Effect of astragalosides on long non-coding RNA expression profiles in rats with adjuvant-induced arthritis
    Article Snippet: .. Reverse transcription-quantitative PCR (RT-qPCR) analysis The total RNA was extracted using TRIzol reagent and then cDNA was synthesized with PrimeScript™ RT reagent kit (Takara Bio, Inc.) at 65°C for 5 min and 42°C for 60 min. RT-qPCR analysis was performed using a LightCycler 480 II (Roche, Germany) with TB Green™ Advantage® qPCR Premix (Takara Bio, Inc.). .. The thermocycling conditions were as follows: Pre-denaturation at 95°C for 5 min, 40 cycles of 95°C for 15 sec and 60°C for 60 sec.

    Quantitative RT-PCR:

    Article Title: Identification and expression of cuticular protein genes based on Locusta migratoria transcriptome
    Article Snippet: .. For reverse-transcription quantitative PCR (RT-qPCR) analysis and SYBR Green kits were used according to the manufacturer’s instructions (TaKaRa, Japan) with specific primers for each gene designed and listed in . .. The total volume of RT-qPCR reactions was 20 μl, containing 10 μl of 2 × SYBR® Premix EX Taq™ (TaKaRa, Japan), 0.4 μl of 50 × ROX Reference Dye (TaKaRa, Japan) and 0.4 μl of specific primers (10 μM), with the following conditions: denaturation at 95 °C for 1 min, followed by 40 cycles at 95 °C for 15 s, 60 °C for 31 s with an ABI 7300 real-time PCR machine (Applied Biosystems, USA) using FastStart Universal SYBR Green Master.

    Article Title: Effect of astragalosides on long non-coding RNA expression profiles in rats with adjuvant-induced arthritis
    Article Snippet: .. Reverse transcription-quantitative PCR (RT-qPCR) analysis The total RNA was extracted using TRIzol reagent and then cDNA was synthesized with PrimeScript™ RT reagent kit (Takara Bio, Inc.) at 65°C for 5 min and 42°C for 60 min. RT-qPCR analysis was performed using a LightCycler 480 II (Roche, Germany) with TB Green™ Advantage® qPCR Premix (Takara Bio, Inc.). .. The thermocycling conditions were as follows: Pre-denaturation at 95°C for 5 min, 40 cycles of 95°C for 15 sec and 60°C for 60 sec.

    Synthesized:

    Article Title: Effect of astragalosides on long non-coding RNA expression profiles in rats with adjuvant-induced arthritis
    Article Snippet: .. Reverse transcription-quantitative PCR (RT-qPCR) analysis The total RNA was extracted using TRIzol reagent and then cDNA was synthesized with PrimeScript™ RT reagent kit (Takara Bio, Inc.) at 65°C for 5 min and 42°C for 60 min. RT-qPCR analysis was performed using a LightCycler 480 II (Roche, Germany) with TB Green™ Advantage® qPCR Premix (Takara Bio, Inc.). .. The thermocycling conditions were as follows: Pre-denaturation at 95°C for 5 min, 40 cycles of 95°C for 15 sec and 60°C for 60 sec.

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    TaKaRa real time quantitative reverse transcription pcr rt qpcr analysis
    Expressional regulation of enzyme genes and regulatory genes by TP05746 in T. pinophilus as demonstrated by <t>RT-qPCR.</t> (A) Amylase genes and their regulatory genes. Total RNA was extracted from fungal strains cultured in medium containing SCS as the carbon source for 12, 24, and 48 h after transfer from glucose. (B) Cellulase and xylanase genes. (C) Genes involved in conidiogenesis. Fungal cells were cultured on WA for 12, 24, and 48 h after transfer from glucose. Expression levels of the genes tested in the Δ TP05746 mutant were normalized against the parental strain Δ TpKu70 . ∗∗ p ≤ 0.01 and ∗ p ≤ 0.05 indicate differences between Δ TP05746 and Δ TpKu70 by Student’s t test. Each experiment contained three biological replicates. RT-qPCR, real-time quantitative reverse transcription <t>PCR;</t> WA, wheat bran plus Avicel.
    Real Time Quantitative Reverse Transcription Pcr Rt Qpcr Analysis, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/real time quantitative reverse transcription pcr rt qpcr analysis/product/TaKaRa
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    real time quantitative reverse transcription pcr rt qpcr analysis - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

    93
    TaKaRa reverse transcription quantitative pcr rt qpcr analysis total rna
    TUDCA alleviates ER stress in SW-13 cells. After 48 h of TUDCA intervention, total <t>RNA</t> was extracted and used to detect the mRNA expression of ER stress-related factors using (A) reverse transcription-quantitative <t>PCR</t> and (B) western blot analysis. (C) Quantification of the western blot analysis. *P
    Reverse Transcription Quantitative Pcr Rt Qpcr Analysis Total Rna, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/reverse transcription quantitative pcr rt qpcr analysis total rna/product/TaKaRa
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    reverse transcription quantitative pcr rt qpcr analysis total rna - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

    Image Search Results


    Expressional regulation of enzyme genes and regulatory genes by TP05746 in T. pinophilus as demonstrated by RT-qPCR. (A) Amylase genes and their regulatory genes. Total RNA was extracted from fungal strains cultured in medium containing SCS as the carbon source for 12, 24, and 48 h after transfer from glucose. (B) Cellulase and xylanase genes. (C) Genes involved in conidiogenesis. Fungal cells were cultured on WA for 12, 24, and 48 h after transfer from glucose. Expression levels of the genes tested in the Δ TP05746 mutant were normalized against the parental strain Δ TpKu70 . ∗∗ p ≤ 0.01 and ∗ p ≤ 0.05 indicate differences between Δ TP05746 and Δ TpKu70 by Student’s t test. Each experiment contained three biological replicates. RT-qPCR, real-time quantitative reverse transcription PCR; WA, wheat bran plus Avicel.

    Journal: Frontiers in Microbiology

    Article Title: Identification of a Novel Transcription Factor TP05746 Involved in Regulating the Production of Plant-Biomass-Degrading Enzymes in Talaromyces pinophilus

    doi: 10.3389/fmicb.2019.02875

    Figure Lengend Snippet: Expressional regulation of enzyme genes and regulatory genes by TP05746 in T. pinophilus as demonstrated by RT-qPCR. (A) Amylase genes and their regulatory genes. Total RNA was extracted from fungal strains cultured in medium containing SCS as the carbon source for 12, 24, and 48 h after transfer from glucose. (B) Cellulase and xylanase genes. (C) Genes involved in conidiogenesis. Fungal cells were cultured on WA for 12, 24, and 48 h after transfer from glucose. Expression levels of the genes tested in the Δ TP05746 mutant were normalized against the parental strain Δ TpKu70 . ∗∗ p ≤ 0.01 and ∗ p ≤ 0.05 indicate differences between Δ TP05746 and Δ TpKu70 by Student’s t test. Each experiment contained three biological replicates. RT-qPCR, real-time quantitative reverse transcription PCR; WA, wheat bran plus Avicel.

    Article Snippet: Real-Time Quantitative Reverse Transcription-PCR (RT-qPCR) Analysis The PrimeScriptTM RT Reagent kit (TaKaRa Bio Inc.) was used to synthesize the first-strand cDNA from total RNA of mutant ΔTpKu70 as the template, according to the manufacturer’s instruction.

    Techniques: Quantitative RT-PCR, Cell Culture, Expressing, Mutagenesis, Polymerase Chain Reaction

    Real-time quantitative PCR analysis and model of Chr11 in YZG-5. ( a ) Diagram of Chr11 with 21 related genes uniformly distributed on the short arm and qPCR results. The level of gene expression differed almost two-fold between Os11g01200-11g10130 and Os11g10120-11g20790. ( b ) Model pattern of the composition of Chr11 in variant YZG-5.

    Journal: Scientific Reports

    Article Title: Segmental Duplication of Chromosome 11 and its Implications for Cell Division and Genome-wide Expression in Rice

    doi: 10.1038/s41598-017-02796-9

    Figure Lengend Snippet: Real-time quantitative PCR analysis and model of Chr11 in YZG-5. ( a ) Diagram of Chr11 with 21 related genes uniformly distributed on the short arm and qPCR results. The level of gene expression differed almost two-fold between Os11g01200-11g10130 and Os11g10120-11g20790. ( b ) Model pattern of the composition of Chr11 in variant YZG-5.

    Article Snippet: Real-time quantitative PCR (qPCR) and reverse transcription quantitative PCR (RT-qPCR) Real-time quantitative PCR analysis was performed using the ABI ViiATM Real Time Quantitative PCR System with SYBR Premix Ex Taq (TaKaRa) and gene-specific primers (Table ).

    Techniques: Real-time Polymerase Chain Reaction, Expressing, Variant Assay

    TUDCA alleviates ER stress in SW-13 cells. After 48 h of TUDCA intervention, total RNA was extracted and used to detect the mRNA expression of ER stress-related factors using (A) reverse transcription-quantitative PCR and (B) western blot analysis. (C) Quantification of the western blot analysis. *P

    Journal: Oncology Letters

    Article Title: Tauroursodeoxycholic acid mediates endoplasmic reticulum stress and autophagy in adrenocortical carcinoma cells

    doi: 10.3892/ol.2019.11057

    Figure Lengend Snippet: TUDCA alleviates ER stress in SW-13 cells. After 48 h of TUDCA intervention, total RNA was extracted and used to detect the mRNA expression of ER stress-related factors using (A) reverse transcription-quantitative PCR and (B) western blot analysis. (C) Quantification of the western blot analysis. *P

    Article Snippet: Reverse transcription-quantitative PCR (RT-qPCR) analysis Total RNA was extracted from SW-13 cells cultured in different concentrations of TUDCA (0, 100, 200, 300, or 400 µM for 48 h) using RNAiso Plus reagent (Takara Biotechnology Co., Ltd.).

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Western Blot

    LncRNA CASC7 acts as a competing endogenous RNA for miR-92a in NSCLC cells. (A) Predicted miR-92a-binding sites on CASC7. (B) The miR-92a expression levels in 80 paired NSCLC and adjacent tissues were determined by RT-qPCR. P

    Journal: International Journal of Oncology

    Article Title: The long non-coding RNA CASC7 inhibits growth and invasion of non-small cell lung cancer cells through phosphatase and tensin homolog upregulation via sequestration of miR-92a

    doi: 10.3892/ijo.2020.5076

    Figure Lengend Snippet: LncRNA CASC7 acts as a competing endogenous RNA for miR-92a in NSCLC cells. (A) Predicted miR-92a-binding sites on CASC7. (B) The miR-92a expression levels in 80 paired NSCLC and adjacent tissues were determined by RT-qPCR. P

    Article Snippet: Reverse transcription-quantitative PCR (RT-qPCR) analysis Total RNA was isolated from NSCLC tissues and cell lines using TRIzol reagent (TaKaRa Bio, Inc.).

    Techniques: Binding Assay, Expressing, Quantitative RT-PCR