reverse transcription kit (Thermo Fisher)
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90
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Name:
RevertAid First Strand cDNA Synthesis Kit
Description:
Thermo Scientific RevertAid First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates The kit uses RevertAid Reverse Transcriptase RT a recombinant M MuLV RT which maintains activity at 42 50°C and is suitable for synthesis of cDNA up to 13 kb RiboLock RNase Inhibitor supplied with the kit effectively protects RNA templates from degradation The RevertAid First Strand cDNA Synthesis Kit is supplied with both oligo dT 18 and random hexamer primers The oligo dT 18 primer anneals selectively to the poly A tail of mRNA Random hexamer primers do not require the presence of the poly A tail therefore they can be used for transcription of the 5 end regions of mRNA or cDNA synthesis of RNA species lacking a poly A tail e g microRNAs Gene specific primers may also be used with the kit Highlights• Full length first strand cDNA up to 13 kb• Optimum reaction temperature 42°C• Complete kit all the components for the RT reaction are includedApplications• First strand cDNA synthesis for RT PCR and RT qPCR• Construction of full length cDNA libraries• Antisense RNA synthesis
Catalog Number:
k1621
Price:
None
Applications:
Cloning|cDNA Libraries & Library Construction
Category:
Kits and Assays
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Structured Review
Thermo Fisher
reverse transcription kit
Thermo Scientific RevertAid First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates The kit uses RevertAid Reverse Transcriptase RT a recombinant M MuLV RT which maintains activity at 42 50°C and is suitable for synthesis of cDNA up to 13 kb RiboLock RNase Inhibitor supplied with the kit effectively protects RNA templates from degradation The RevertAid First Strand cDNA Synthesis Kit is supplied with both oligo dT 18 and random hexamer primers The oligo dT 18 primer anneals selectively to the poly A tail of mRNA Random hexamer primers do not require the presence of the poly A tail therefore they can be used for transcription of the 5 end regions of mRNA or cDNA synthesis of RNA species lacking a poly A tail e g microRNAs Gene specific primers may also be used with the kit Highlights• Full length first strand cDNA up to 13 kb• Optimum reaction temperature 42°C• Complete kit all the components for the RT reaction are includedApplications• First strand cDNA synthesis for RT PCR and RT qPCR• Construction of full length cDNA libraries• Antisense RNA synthesis
https://www.bioz.com/result/reverse transcription kit/product/Thermo Fisher
Average 90 stars, based on 241 article reviews
Price from $9.99 to $1999.99
Thermo Scientific RevertAid First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates The kit uses RevertAid Reverse Transcriptase RT a recombinant M MuLV RT which maintains activity at 42 50°C and is suitable for synthesis of cDNA up to 13 kb RiboLock RNase Inhibitor supplied with the kit effectively protects RNA templates from degradation The RevertAid First Strand cDNA Synthesis Kit is supplied with both oligo dT 18 and random hexamer primers The oligo dT 18 primer anneals selectively to the poly A tail of mRNA Random hexamer primers do not require the presence of the poly A tail therefore they can be used for transcription of the 5 end regions of mRNA or cDNA synthesis of RNA species lacking a poly A tail e g microRNAs Gene specific primers may also be used with the kit Highlights• Full length first strand cDNA up to 13 kb• Optimum reaction temperature 42°C• Complete kit all the components for the RT reaction are includedApplications• First strand cDNA synthesis for RT PCR and RT qPCR• Construction of full length cDNA libraries• Antisense RNA synthesis
https://www.bioz.com/result/reverse transcription kit/product/Thermo Fisher
Average 90 stars, based on 241 article reviews
Price from $9.99 to $1999.99
reverse transcription kit - by Bioz Stars,
2020-08
90/100 stars
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Staining:Article Title: Evolutionarily novel genes are expressed in transgenic fish tumors and their orthologs are involved in development of progressive traits in humans Article Snippet: .. Ethidium bromide staining of RNA in agarose gels visualizes two predominant bands of small and large ribosomal RNA, a bands of low molecular mass RNA. cDNA synthesis was performed from equal amounts of RNA using Spectrophotometry:Article Title: Anti-Inflammatory Effect of IL-37-Producing T-Cell Population in DSS-Induced Chronic Inflammatory Bowel Disease in Mice Article Snippet: .. The Nanodrop spectrophotometer (Thermo Scientific, Waltham, MA, USA) was used to measure absorbance. cDNAs were synthesized by using the Synthesized:Article Title: Anti-Inflammatory Effect of IL-37-Producing T-Cell Population in DSS-Induced Chronic Inflammatory Bowel Disease in Mice Article Snippet: .. The Nanodrop spectrophotometer (Thermo Scientific, Waltham, MA, USA) was used to measure absorbance. cDNAs were synthesized by using the Article Title: Involvement of CD147 on multidrug resistance through the regulation of P-glycoprotein expression in K562/ADR leukemic cell line Article Snippet: .. Single-stranded cDNA was synthesized by reverse transcription from 1 μg of total RNA using the |
![Biogenesis of microRNA-548ac and genetic variants in the CD58 gene locus. ( A ) Diagram of the processing of an mRNA and an intronic miRNA from the same transcript (adapted from [ 49 ]). Both RNA splicing by the spliceosome and miRNA stem-loop cropping by the Drosha-DGCR8 complex occur cotranscriptionally. Drosha may cleave the miRNA-harboring intron before splicing commitment of the flanking exons. The resulting precursor miRNA is subsequently processed into a mature miRNA, which is loaded into the RNA-induced silencing complex (RISC). ( B ) Annotated secondary structure of hsa-mir-548ac. Highlighted in gray is the 22 nt long sequence of the mature miRNA isoform as assigned by Jima et al . (miRBase accession MIMAT0018938) [ 43 ]. The red circle marks the only common single-nucleotide polymorphism (SNP) within the stem-loop region. The G allele is overrepresented in MS patients. ( C ) Genetic variants in pairwise linkage disequilibrium (LD) with SNP rs1414273. This plot was generated using the LDproxy module of the web-based analysis tool LDlink [ 97 ]. Shown are r 2 LD values of proximal SNPs based on all subpopulations of the 1000 Genomes project (orange dots), recombination rate as estimated from HapMap data (gray line), and the position and exon-intron structure of nearby genes on chromosome 1 (chr1, GRCh37 assembly). The MS-associated SNP rs1335532 is in strong LD with SNP rs1414273 (blue) (correlated forward strand alleles: A = C, G = T). The entire block of LD spans about 50 kb but does not include the promoter region of CD58, which is encoded on the minus strand in the reference genome. ( D ) Worldwide distribution of SNP rs1335532 alleles. Global allele frequencies were visualized as two-color pie charts with the HGDP Selection Browser [ 98 ]. The disease susceptibility variant (A, blue) is the major allele in European populations and the minor allele in East Asian and Southern African populations. cM/Mb = centimorgan per megabase, HGDP = Human Genome Diversity Panel, MS = multiple sclerosis.](https://storage.googleapis.com/bioz_article_images/PMC6382214/pgen.1007961.g001.jpg)
