reverse transcription kit (Thermo Fisher)
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Name:
Arcturus Paradise PLUS Whole Transcript Reverse Transcription Kit WT RT
Description:
The ARCTURUS Paradise PLUS Whole Transcript RT WT RT Reagent System provides an efficient and reliable solution for purifying RNA from archived Formalin Fixed Paraffin Embedded FFPE tissue samples followed by reverse transcription of whole transcript for use in real time PCR FFPE samples introduce unique challenges for gene expression profiling and gene expression quantitation including chemical modification and fragmentation of RNA molecules Storage of FFPE samples adds to these challenges through increased RNA degradation over time The Paradise PLUS WT RT Reagent System was specifically developed and specially optimized to provide high quality RNA and robust reverse transcription RT from highly fragmented RNA obtained from archived FFPE samples over 20 years old Figure 2 Key product features • Simple workflow minimal sample handling reduces chance of contamination Figure 3 • Efficient RNA extraction and isolation saves time and minimizes sample loss• Optimized RT reactions generate cDNA representing the entire length of the transcript• Accurate transcript analysis results represent high medium and low abundance expressing genesOptimized RNA Extraction and Isolation from Archived FFPE TissuesFigure 1 shows a comparison between total RNA yields using the Paradise PLUS WT RT Reagent System and a similar commercially available protocol The average yield of the three replicate isolations illustrates the abundance of total RNA made available using the Paradise PLUS WT RT Reagent System The Paradise PLUS WT RT Reagent System produces abundant total RNA for reverse transcription and subsequent qPCR analysis up to three times more total RNA than obtained when a similar commercially available product was used for the same sample Superior Amplification Possible Over Wide Range of TargetsThe sensitivity of the Paradise PLUS WT RT Reagent System was compared to similar reagent products available on the market that generate cDNA for use in real time PCR reactions As Figure 4 illustrates improved Ct values were seen for all high medium and low expressing genes when the Paradise PLUS product was used Exon spanning primers at varying distances from the 3 end of the transcript 500 bp to 5 kb were used to show increased sensitivity and improved transcription success compared to other commercially available products The Paradise PLUS WT RT Reagent System successfully amplified all genes suggesting the reagents provided in the kit are optimized to work together saving you time and effort while producing high quality results across the entire transcript Integrated Systems for MicrogenomicsThe Paradise PLUS Reagent System is validated as part of Arcturus complete Systems for Microgenomics an integrated solution for utilizing small quantities of RNA for gene expression analysis and features the ARCTURUSXT Laser Capture Microdissection LCM Instrument to procure pure cell populations ARCTURUS Systems for Microgenomics permit accurate and sensitive microarray assays that reveal molecular otherwise obscured in whole tissue samples or cell mixtures For Research Use Only Not for use in diagnostics procedures
Catalog Number:
kit0305
Price:
None
Applications:
Laser Capture Microdissection|PCR & Real-Time PCR|Reverse Transcription|Gene Expression Analysis & Genotyping|LCM Kits & Reagents
Category:
Kits and Assays
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Structured Review
Thermo Fisher
reverse transcription kit
The ARCTURUS Paradise PLUS Whole Transcript RT WT RT Reagent System provides an efficient and reliable solution for purifying RNA from archived Formalin Fixed Paraffin Embedded FFPE tissue samples followed by reverse transcription of whole transcript for use in real time PCR FFPE samples introduce unique challenges for gene expression profiling and gene expression quantitation including chemical modification and fragmentation of RNA molecules Storage of FFPE samples adds to these challenges through increased RNA degradation over time The Paradise PLUS WT RT Reagent System was specifically developed and specially optimized to provide high quality RNA and robust reverse transcription RT from highly fragmented RNA obtained from archived FFPE samples over 20 years old Figure 2 Key product features • Simple workflow minimal sample handling reduces chance of contamination Figure 3 • Efficient RNA extraction and isolation saves time and minimizes sample loss• Optimized RT reactions generate cDNA representing the entire length of the transcript• Accurate transcript analysis results represent high medium and low abundance expressing genesOptimized RNA Extraction and Isolation from Archived FFPE TissuesFigure 1 shows a comparison between total RNA yields using the Paradise PLUS WT RT Reagent System and a similar commercially available protocol The average yield of the three replicate isolations illustrates the abundance of total RNA made available using the Paradise PLUS WT RT Reagent System The Paradise PLUS WT RT Reagent System produces abundant total RNA for reverse transcription and subsequent qPCR analysis up to three times more total RNA than obtained when a similar commercially available product was used for the same sample Superior Amplification Possible Over Wide Range of TargetsThe sensitivity of the Paradise PLUS WT RT Reagent System was compared to similar reagent products available on the market that generate cDNA for use in real time PCR reactions As Figure 4 illustrates improved Ct values were seen for all high medium and low expressing genes when the Paradise PLUS product was used Exon spanning primers at varying distances from the 3 end of the transcript 500 bp to 5 kb were used to show increased sensitivity and improved transcription success compared to other commercially available products The Paradise PLUS WT RT Reagent System successfully amplified all genes suggesting the reagents provided in the kit are optimized to work together saving you time and effort while producing high quality results across the entire transcript Integrated Systems for MicrogenomicsThe Paradise PLUS Reagent System is validated as part of Arcturus complete Systems for Microgenomics an integrated solution for utilizing small quantities of RNA for gene expression analysis and features the ARCTURUSXT Laser Capture Microdissection LCM Instrument to procure pure cell populations ARCTURUS Systems for Microgenomics permit accurate and sensitive microarray assays that reveal molecular otherwise obscured in whole tissue samples or cell mixtures For Research Use Only Not for use in diagnostics procedures
https://www.bioz.com/result/reverse transcription kit/product/Thermo Fisher
Average 99 stars, based on 241 article reviews
Price from $9.99 to $1999.99
The ARCTURUS Paradise PLUS Whole Transcript RT WT RT Reagent System provides an efficient and reliable solution for purifying RNA from archived Formalin Fixed Paraffin Embedded FFPE tissue samples followed by reverse transcription of whole transcript for use in real time PCR FFPE samples introduce unique challenges for gene expression profiling and gene expression quantitation including chemical modification and fragmentation of RNA molecules Storage of FFPE samples adds to these challenges through increased RNA degradation over time The Paradise PLUS WT RT Reagent System was specifically developed and specially optimized to provide high quality RNA and robust reverse transcription RT from highly fragmented RNA obtained from archived FFPE samples over 20 years old Figure 2 Key product features • Simple workflow minimal sample handling reduces chance of contamination Figure 3 • Efficient RNA extraction and isolation saves time and minimizes sample loss• Optimized RT reactions generate cDNA representing the entire length of the transcript• Accurate transcript analysis results represent high medium and low abundance expressing genesOptimized RNA Extraction and Isolation from Archived FFPE TissuesFigure 1 shows a comparison between total RNA yields using the Paradise PLUS WT RT Reagent System and a similar commercially available protocol The average yield of the three replicate isolations illustrates the abundance of total RNA made available using the Paradise PLUS WT RT Reagent System The Paradise PLUS WT RT Reagent System produces abundant total RNA for reverse transcription and subsequent qPCR analysis up to three times more total RNA than obtained when a similar commercially available product was used for the same sample Superior Amplification Possible Over Wide Range of TargetsThe sensitivity of the Paradise PLUS WT RT Reagent System was compared to similar reagent products available on the market that generate cDNA for use in real time PCR reactions As Figure 4 illustrates improved Ct values were seen for all high medium and low expressing genes when the Paradise PLUS product was used Exon spanning primers at varying distances from the 3 end of the transcript 500 bp to 5 kb were used to show increased sensitivity and improved transcription success compared to other commercially available products The Paradise PLUS WT RT Reagent System successfully amplified all genes suggesting the reagents provided in the kit are optimized to work together saving you time and effort while producing high quality results across the entire transcript Integrated Systems for MicrogenomicsThe Paradise PLUS Reagent System is validated as part of Arcturus complete Systems for Microgenomics an integrated solution for utilizing small quantities of RNA for gene expression analysis and features the ARCTURUSXT Laser Capture Microdissection LCM Instrument to procure pure cell populations ARCTURUS Systems for Microgenomics permit accurate and sensitive microarray assays that reveal molecular otherwise obscured in whole tissue samples or cell mixtures For Research Use Only Not for use in diagnostics procedures
https://www.bioz.com/result/reverse transcription kit/product/Thermo Fisher
Average 99 stars, based on 241 article reviews
Price from $9.99 to $1999.99
reverse transcription kit - by Bioz Stars,
2021-01
99/100 stars
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SYBR Green Assay:Article Title: MicroRNA-93 contributes to the suppression of lung inflammatory responses in LPS-induced acute lung injury in mice via the TLR4/MyD88/NF-κB signaling pathway Article Snippet: .. The reverse transcription of miR-93 was performed using the miScript II RT kit and the reverse Article Title: Characterization of the Entire Cystatin Gene Family in Barley and Their Target Cathepsin L-Like Cysteine-Proteases, Partners in the Hordein Mobilization during Seed Germination 1Characterization of the Entire Cystatin Gene Family in Barley and Their Target Cathepsin L-Like Cysteine-Proteases, Partners in the Hordein Mobilization during Seed Germination 1 [W] Article Snippet: .. Total RNA was extracted from frozen samples by the phenol/chloroform method, followed by precipitation with 3 m LiCl ( ) and digestion with DNase. cDNAs were synthesized from 1 μ g of RNA using the Synthesized:Article Title: Effect of intravenous immunoglobulin on the function of Treg cells derived from immunosuppressed mice with Pseudomonas aeruginosa pneumonia Article Snippet: .. Total cDNA was produced by reverse transcription via a Thermo Fisher Article Title: Characterization of the Entire Cystatin Gene Family in Barley and Their Target Cathepsin L-Like Cysteine-Proteases, Partners in the Hordein Mobilization during Seed Germination 1Characterization of the Entire Cystatin Gene Family in Barley and Their Target Cathepsin L-Like Cysteine-Proteases, Partners in the Hordein Mobilization during Seed Germination 1 [W] Article Snippet: .. Total RNA was extracted from frozen samples by the phenol/chloroform method, followed by precipitation with 3 m LiCl ( ) and digestion with DNase. cDNAs were synthesized from 1 μ g of RNA using the Isolation:Article Title: Treatment with adipose tissue-derived mesenchymal stem cells exerts anti-diabetic effects, improves long-term complications, and attenuates inflammation in type 2 diabetic rats Article Snippet: .. Quantitative real-time PCR Total RNA was isolated from rat adipose tissue, liver tissue, renal tissue, and lung tissue using Trizol reagent (Life technologies, Frederick, USA) and was reverse-transcribed with a Quantitative RT-PCR:Article Title: Effect of intravenous immunoglobulin on the function of Treg cells derived from immunosuppressed mice with Pseudomonas aeruginosa pneumonia Article Snippet: .. Total cDNA was produced by reverse transcription via a Thermo Fisher Article Title: Characterization of the Entire Cystatin Gene Family in Barley and Their Target Cathepsin L-Like Cysteine-Proteases, Partners in the Hordein Mobilization during Seed Germination 1Characterization of the Entire Cystatin Gene Family in Barley and Their Target Cathepsin L-Like Cysteine-Proteases, Partners in the Hordein Mobilization during Seed Germination 1 [W] Article Snippet: .. Total RNA was extracted from frozen samples by the phenol/chloroform method, followed by precipitation with 3 m LiCl ( ) and digestion with DNase. cDNAs were synthesized from 1 μ g of RNA using the Produced:Article Title: Effect of intravenous immunoglobulin on the function of Treg cells derived from immunosuppressed mice with Pseudomonas aeruginosa pneumonia Article Snippet: .. Total cDNA was produced by reverse transcription via a Thermo Fisher Concentration Assay:Article Title: Tryptase and Protease-Activated Receptor 2 Expression Levels in Irritable Bowel Syndrome Article Snippet: .. Total RNA was extracted, and reversed by using Polymerase Chain Reaction:Article Title: Effect of intravenous immunoglobulin on the function of Treg cells derived from immunosuppressed mice with Pseudomonas aeruginosa pneumonia Article Snippet: .. Total cDNA was produced by reverse transcription via a Thermo Fisher Expressing:Article Title: MicroRNA-93 contributes to the suppression of lung inflammatory responses in LPS-induced acute lung injury in mice via the TLR4/MyD88/NF-κB signaling pathway Article Snippet: .. The reverse transcription of miR-93 was performed using the miScript II RT kit and the reverse Article Title: Rho/ROCK-dependent inhibition of 3T3-L1 adipogenesis by G-protein-deamidating dermonecrotic toxins: differential regulation of Notch1, Pref1/Dlk1, and ?-catenin signaling Article Snippet: .. The levels of mRNA expression were determined by RT-PCR using Reverse Transcription Polymerase Chain Reaction:Article Title: Rho/ROCK-dependent inhibition of 3T3-L1 adipogenesis by G-protein-deamidating dermonecrotic toxins: differential regulation of Notch1, Pref1/Dlk1, and ?-catenin signaling Article Snippet: .. The levels of mRNA expression were determined by RT-PCR using Real-time Polymerase Chain Reaction:Article Title: Treatment with adipose tissue-derived mesenchymal stem cells exerts anti-diabetic effects, improves long-term complications, and attenuates inflammation in type 2 diabetic rats Article Snippet: .. Quantitative real-time PCR Total RNA was isolated from rat adipose tissue, liver tissue, renal tissue, and lung tissue using Trizol reagent (Life technologies, Frederick, USA) and was reverse-transcribed with a Article Title: Characterization of the Entire Cystatin Gene Family in Barley and Their Target Cathepsin L-Like Cysteine-Proteases, Partners in the Hordein Mobilization during Seed Germination 1Characterization of the Entire Cystatin Gene Family in Barley and Their Target Cathepsin L-Like Cysteine-Proteases, Partners in the Hordein Mobilization during Seed Germination 1 [W] Article Snippet: .. Total RNA was extracted from frozen samples by the phenol/chloroform method, followed by precipitation with 3 m LiCl ( ) and digestion with DNase. cDNAs were synthesized from 1 μ g of RNA using the |
![Biogenesis of microRNA-548ac and genetic variants in the CD58 gene locus. ( A ) Diagram of the processing of an mRNA and an intronic miRNA from the same transcript (adapted from [ 49 ]). Both RNA splicing by the spliceosome and miRNA stem-loop cropping by the Drosha-DGCR8 complex occur cotranscriptionally. Drosha may cleave the miRNA-harboring intron before splicing commitment of the flanking exons. The resulting precursor miRNA is subsequently processed into a mature miRNA, which is loaded into the RNA-induced silencing complex (RISC). ( B ) Annotated secondary structure of hsa-mir-548ac. Highlighted in gray is the 22 nt long sequence of the mature miRNA isoform as assigned by Jima et al . (miRBase accession MIMAT0018938) [ 43 ]. The red circle marks the only common single-nucleotide polymorphism (SNP) within the stem-loop region. The G allele is overrepresented in MS patients. ( C ) Genetic variants in pairwise linkage disequilibrium (LD) with SNP rs1414273. This plot was generated using the LDproxy module of the web-based analysis tool LDlink [ 97 ]. Shown are r 2 LD values of proximal SNPs based on all subpopulations of the 1000 Genomes project (orange dots), recombination rate as estimated from HapMap data (gray line), and the position and exon-intron structure of nearby genes on chromosome 1 (chr1, GRCh37 assembly). The MS-associated SNP rs1335532 is in strong LD with SNP rs1414273 (blue) (correlated forward strand alleles: A = C, G = T). The entire block of LD spans about 50 kb but does not include the promoter region of CD58, which is encoded on the minus strand in the reference genome. ( D ) Worldwide distribution of SNP rs1335532 alleles. Global allele frequencies were visualized as two-color pie charts with the HGDP Selection Browser [ 98 ]. The disease susceptibility variant (A, blue) is the major allele in European populations and the minor allele in East Asian and Southern African populations. cM/Mb = centimorgan per megabase, HGDP = Human Genome Diversity Panel, MS = multiple sclerosis.](https://storage.googleapis.com/bioz_article_images/PMC6382214/pgen.1007961.g001.jpg)
