reverse transcription kit (Thermo Fisher)

Name:
Arcturus Paradise PLUS Whole Transcript Reverse Transcription Kit WT RT
Description:
The ARCTURUS Paradise PLUS Whole Transcript RT WT RT Reagent System provides an efficient and reliable solution for purifying RNA from archived Formalin Fixed Paraffin Embedded FFPE tissue samples followed by reverse transcription of whole transcript for use in real time PCR FFPE samples introduce unique challenges for gene expression profiling and gene expression quantitation including chemical modification and fragmentation of RNA molecules Storage of FFPE samples adds to these challenges through increased RNA degradation over time The Paradise PLUS WT RT Reagent System was specifically developed and specially optimized to provide high quality RNA and robust reverse transcription RT from highly fragmented RNA obtained from archived FFPE samples over 20 years old Figure 2 Key product features • Simple workflow minimal sample handling reduces chance of contamination Figure 3 • Efficient RNA extraction and isolation saves time and minimizes sample loss• Optimized RT reactions generate cDNA representing the entire length of the transcript• Accurate transcript analysis results represent high medium and low abundance expressing genesOptimized RNA Extraction and Isolation from Archived FFPE TissuesFigure 1 shows a comparison between total RNA yields using the Paradise PLUS WT RT Reagent System and a similar commercially available protocol The average yield of the three replicate isolations illustrates the abundance of total RNA made available using the Paradise PLUS WT RT Reagent System The Paradise PLUS WT RT Reagent System produces abundant total RNA for reverse transcription and subsequent qPCR analysis up to three times more total RNA than obtained when a similar commercially available product was used for the same sample Superior Amplification Possible Over Wide Range of TargetsThe sensitivity of the Paradise PLUS WT RT Reagent System was compared to similar reagent products available on the market that generate cDNA for use in real time PCR reactions As Figure 4 illustrates improved Ct values were seen for all high medium and low expressing genes when the Paradise PLUS product was used Exon spanning primers at varying distances from the 3 end of the transcript 500 bp to 5 kb were used to show increased sensitivity and improved transcription success compared to other commercially available products The Paradise PLUS WT RT Reagent System successfully amplified all genes suggesting the reagents provided in the kit are optimized to work together saving you time and effort while producing high quality results across the entire transcript Integrated Systems for MicrogenomicsThe Paradise PLUS Reagent System is validated as part of Arcturus complete Systems for Microgenomics an integrated solution for utilizing small quantities of RNA for gene expression analysis and features the ARCTURUSXT Laser Capture Microdissection LCM Instrument to procure pure cell populations ARCTURUS Systems for Microgenomics permit accurate and sensitive microarray assays that reveal molecular otherwise obscured in whole tissue samples or cell mixtures For Research Use Only Not for use in diagnostics procedures
Catalog Number:
kit0305
Price:
None
Applications:
Laser Capture Microdissection|PCR & Real-Time PCR|Reverse Transcription|Gene Expression Analysis & Genotyping|LCM Kits & Reagents
Category:
Kits and Assays
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Structured Review
The ARCTURUS Paradise PLUS Whole Transcript RT WT RT Reagent System provides an efficient and reliable solution for purifying RNA from archived Formalin Fixed Paraffin Embedded FFPE tissue samples followed by reverse transcription of whole transcript for use in real time PCR FFPE samples introduce unique challenges for gene expression profiling and gene expression quantitation including chemical modification and fragmentation of RNA molecules Storage of FFPE samples adds to these challenges through increased RNA degradation over time The Paradise PLUS WT RT Reagent System was specifically developed and specially optimized to provide high quality RNA and robust reverse transcription RT from highly fragmented RNA obtained from archived FFPE samples over 20 years old Figure 2 Key product features • Simple workflow minimal sample handling reduces chance of contamination Figure 3 • Efficient RNA extraction and isolation saves time and minimizes sample loss• Optimized RT reactions generate cDNA representing the entire length of the transcript• Accurate transcript analysis results represent high medium and low abundance expressing genesOptimized RNA Extraction and Isolation from Archived FFPE TissuesFigure 1 shows a comparison between total RNA yields using the Paradise PLUS WT RT Reagent System and a similar commercially available protocol The average yield of the three replicate isolations illustrates the abundance of total RNA made available using the Paradise PLUS WT RT Reagent System The Paradise PLUS WT RT Reagent System produces abundant total RNA for reverse transcription and subsequent qPCR analysis up to three times more total RNA than obtained when a similar commercially available product was used for the same sample Superior Amplification Possible Over Wide Range of TargetsThe sensitivity of the Paradise PLUS WT RT Reagent System was compared to similar reagent products available on the market that generate cDNA for use in real time PCR reactions As Figure 4 illustrates improved Ct values were seen for all high medium and low expressing genes when the Paradise PLUS product was used Exon spanning primers at varying distances from the 3 end of the transcript 500 bp to 5 kb were used to show increased sensitivity and improved transcription success compared to other commercially available products The Paradise PLUS WT RT Reagent System successfully amplified all genes suggesting the reagents provided in the kit are optimized to work together saving you time and effort while producing high quality results across the entire transcript Integrated Systems for MicrogenomicsThe Paradise PLUS Reagent System is validated as part of Arcturus complete Systems for Microgenomics an integrated solution for utilizing small quantities of RNA for gene expression analysis and features the ARCTURUSXT Laser Capture Microdissection LCM Instrument to procure pure cell populations ARCTURUS Systems for Microgenomics permit accurate and sensitive microarray assays that reveal molecular otherwise obscured in whole tissue samples or cell mixtures For Research Use Only Not for use in diagnostics procedures
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SYBR Green Assay:Article Title: MicroRNA-93 contributes to the suppression of lung inflammatory responses in LPS-induced acute lung injury in mice via the TLR4/MyD88/NF-κB signaling pathway Article Snippet: .. The reverse transcription of miR-93 was performed using the miScript II RT kit and the reverse Article Title: Characterization of the Entire Cystatin Gene Family in Barley and Their Target Cathepsin L-Like Cysteine-Proteases, Partners in the Hordein Mobilization during Seed Germination 1Characterization of the Entire Cystatin Gene Family in Barley and Their Target Cathepsin L-Like Cysteine-Proteases, Partners in the Hordein Mobilization during Seed Germination 1 [W] Article Snippet: .. Total RNA was extracted from frozen samples by the phenol/chloroform method, followed by precipitation with 3 m LiCl ( ) and digestion with DNase. cDNAs were synthesized from 1 μ g of RNA using the Synthesized:Article Title: Effect of intravenous immunoglobulin on the function of Treg cells derived from immunosuppressed mice with Pseudomonas aeruginosa pneumonia Article Snippet: .. Total cDNA was produced by reverse transcription via a Thermo Fisher Article Title: Characterization of the Entire Cystatin Gene Family in Barley and Their Target Cathepsin L-Like Cysteine-Proteases, Partners in the Hordein Mobilization during Seed Germination 1Characterization of the Entire Cystatin Gene Family in Barley and Their Target Cathepsin L-Like Cysteine-Proteases, Partners in the Hordein Mobilization during Seed Germination 1 [W] Article Snippet: .. Total RNA was extracted from frozen samples by the phenol/chloroform method, followed by precipitation with 3 m LiCl ( ) and digestion with DNase. cDNAs were synthesized from 1 μ g of RNA using the Isolation:Article Title: Treatment with adipose tissue-derived mesenchymal stem cells exerts anti-diabetic effects, improves long-term complications, and attenuates inflammation in type 2 diabetic rats Article Snippet: .. Quantitative real-time PCR Total RNA was isolated from rat adipose tissue, liver tissue, renal tissue, and lung tissue using Trizol reagent (Life technologies, Frederick, USA) and was reverse-transcribed with a Quantitative RT-PCR:Article Title: Effect of intravenous immunoglobulin on the function of Treg cells derived from immunosuppressed mice with Pseudomonas aeruginosa pneumonia Article Snippet: .. Total cDNA was produced by reverse transcription via a Thermo Fisher Article Title: Characterization of the Entire Cystatin Gene Family in Barley and Their Target Cathepsin L-Like Cysteine-Proteases, Partners in the Hordein Mobilization during Seed Germination 1Characterization of the Entire Cystatin Gene Family in Barley and Their Target Cathepsin L-Like Cysteine-Proteases, Partners in the Hordein Mobilization during Seed Germination 1 [W] Article Snippet: .. Total RNA was extracted from frozen samples by the phenol/chloroform method, followed by precipitation with 3 m LiCl ( ) and digestion with DNase. cDNAs were synthesized from 1 μ g of RNA using the Produced:Article Title: Effect of intravenous immunoglobulin on the function of Treg cells derived from immunosuppressed mice with Pseudomonas aeruginosa pneumonia Article Snippet: .. Total cDNA was produced by reverse transcription via a Thermo Fisher Concentration Assay:Article Title: Tryptase and Protease-Activated Receptor 2 Expression Levels in Irritable Bowel Syndrome Article Snippet: .. Total RNA was extracted, and reversed by using Polymerase Chain Reaction:Article Title: Effect of intravenous immunoglobulin on the function of Treg cells derived from immunosuppressed mice with Pseudomonas aeruginosa pneumonia Article Snippet: .. Total cDNA was produced by reverse transcription via a Thermo Fisher Expressing:Article Title: MicroRNA-93 contributes to the suppression of lung inflammatory responses in LPS-induced acute lung injury in mice via the TLR4/MyD88/NF-κB signaling pathway Article Snippet: .. The reverse transcription of miR-93 was performed using the miScript II RT kit and the reverse Article Title: Rho/ROCK-dependent inhibition of 3T3-L1 adipogenesis by G-protein-deamidating dermonecrotic toxins: differential regulation of Notch1, Pref1/Dlk1, and ?-catenin signaling Article Snippet: .. The levels of mRNA expression were determined by RT-PCR using Reverse Transcription Polymerase Chain Reaction:Article Title: Rho/ROCK-dependent inhibition of 3T3-L1 adipogenesis by G-protein-deamidating dermonecrotic toxins: differential regulation of Notch1, Pref1/Dlk1, and ?-catenin signaling Article Snippet: .. The levels of mRNA expression were determined by RT-PCR using Real-time Polymerase Chain Reaction:Article Title: Treatment with adipose tissue-derived mesenchymal stem cells exerts anti-diabetic effects, improves long-term complications, and attenuates inflammation in type 2 diabetic rats Article Snippet: .. Quantitative real-time PCR Total RNA was isolated from rat adipose tissue, liver tissue, renal tissue, and lung tissue using Trizol reagent (Life technologies, Frederick, USA) and was reverse-transcribed with a Article Title: Characterization of the Entire Cystatin Gene Family in Barley and Their Target Cathepsin L-Like Cysteine-Proteases, Partners in the Hordein Mobilization during Seed Germination 1Characterization of the Entire Cystatin Gene Family in Barley and Their Target Cathepsin L-Like Cysteine-Proteases, Partners in the Hordein Mobilization during Seed Germination 1 [W] Article Snippet: .. Total RNA was extracted from frozen samples by the phenol/chloroform method, followed by precipitation with 3 m LiCl ( ) and digestion with DNase. cDNAs were synthesized from 1 μ g of RNA using the |