reverse transcription kit superscriptii  (Thermo Fisher)


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    Structured Review

    Thermo Fisher reverse transcription kit superscriptii
    Reverse Transcription Kit Superscriptii, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/reverse transcription kit superscriptii/product/Thermo Fisher
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    reverse transcription kit superscriptii - by Bioz Stars, 2020-04
    95/100 stars

    Related Products / Commonly Used Together

    rna extraction
    trizol
    memantine
    lps
    qpcr
    cdna
    sybr green

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    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: The effect of memantine, an antagonist of the NMDA glutamate receptor, in in vitro and in vivo infections by Trypanosoma cruzi
    Article Snippet: .. Evaluation of the gene expression of inducible NO synthase in RAW 264.7 macrophages To evaluate the gene expression of inducible nitric oxide synthase, 2 x 106 cells per well were cultured for 18 h in 6-well plates in RPMI medium (10% FCS) in the presence of different concentrations of memantine (ranging from 1 to 100 μM) or none (control) and were stimulated by 10 μg/ml LPS for 18 h. After the incubation time, the supernatant was discarded, and the adhered cells were homogenized with Trizol for RNA extraction (Thermo Fisher Scientific). cDNA was synthetized using the Reverse Transcription Kit SuperScriptII (Thermo Fisher Scientific). qPCR was performed with SYBR Green (Fermentas) for detecting the gene expression levels of iNOS. .. All reactions were run in triplicate on an Eppendorf RealPlex Real Time PCR System (Eppendorf) with the standard thermal cycling conditions.

    RNA Extraction:

    Article Title: The effect of memantine, an antagonist of the NMDA glutamate receptor, in in vitro and in vivo infections by Trypanosoma cruzi
    Article Snippet: .. Evaluation of the gene expression of inducible NO synthase in RAW 264.7 macrophages To evaluate the gene expression of inducible nitric oxide synthase, 2 x 106 cells per well were cultured for 18 h in 6-well plates in RPMI medium (10% FCS) in the presence of different concentrations of memantine (ranging from 1 to 100 μM) or none (control) and were stimulated by 10 μg/ml LPS for 18 h. After the incubation time, the supernatant was discarded, and the adhered cells were homogenized with Trizol for RNA extraction (Thermo Fisher Scientific). cDNA was synthetized using the Reverse Transcription Kit SuperScriptII (Thermo Fisher Scientific). qPCR was performed with SYBR Green (Fermentas) for detecting the gene expression levels of iNOS. .. All reactions were run in triplicate on an Eppendorf RealPlex Real Time PCR System (Eppendorf) with the standard thermal cycling conditions.

    Cell Culture:

    Article Title: The effect of memantine, an antagonist of the NMDA glutamate receptor, in in vitro and in vivo infections by Trypanosoma cruzi
    Article Snippet: .. Evaluation of the gene expression of inducible NO synthase in RAW 264.7 macrophages To evaluate the gene expression of inducible nitric oxide synthase, 2 x 106 cells per well were cultured for 18 h in 6-well plates in RPMI medium (10% FCS) in the presence of different concentrations of memantine (ranging from 1 to 100 μM) or none (control) and were stimulated by 10 μg/ml LPS for 18 h. After the incubation time, the supernatant was discarded, and the adhered cells were homogenized with Trizol for RNA extraction (Thermo Fisher Scientific). cDNA was synthetized using the Reverse Transcription Kit SuperScriptII (Thermo Fisher Scientific). qPCR was performed with SYBR Green (Fermentas) for detecting the gene expression levels of iNOS. .. All reactions were run in triplicate on an Eppendorf RealPlex Real Time PCR System (Eppendorf) with the standard thermal cycling conditions.

    SYBR Green Assay:

    Article Title: The effect of memantine, an antagonist of the NMDA glutamate receptor, in in vitro and in vivo infections by Trypanosoma cruzi
    Article Snippet: .. Evaluation of the gene expression of inducible NO synthase in RAW 264.7 macrophages To evaluate the gene expression of inducible nitric oxide synthase, 2 x 106 cells per well were cultured for 18 h in 6-well plates in RPMI medium (10% FCS) in the presence of different concentrations of memantine (ranging from 1 to 100 μM) or none (control) and were stimulated by 10 μg/ml LPS for 18 h. After the incubation time, the supernatant was discarded, and the adhered cells were homogenized with Trizol for RNA extraction (Thermo Fisher Scientific). cDNA was synthetized using the Reverse Transcription Kit SuperScriptII (Thermo Fisher Scientific). qPCR was performed with SYBR Green (Fermentas) for detecting the gene expression levels of iNOS. .. All reactions were run in triplicate on an Eppendorf RealPlex Real Time PCR System (Eppendorf) with the standard thermal cycling conditions.

    Incubation:

    Article Title: The effect of memantine, an antagonist of the NMDA glutamate receptor, in in vitro and in vivo infections by Trypanosoma cruzi
    Article Snippet: .. Evaluation of the gene expression of inducible NO synthase in RAW 264.7 macrophages To evaluate the gene expression of inducible nitric oxide synthase, 2 x 106 cells per well were cultured for 18 h in 6-well plates in RPMI medium (10% FCS) in the presence of different concentrations of memantine (ranging from 1 to 100 μM) or none (control) and were stimulated by 10 μg/ml LPS for 18 h. After the incubation time, the supernatant was discarded, and the adhered cells were homogenized with Trizol for RNA extraction (Thermo Fisher Scientific). cDNA was synthetized using the Reverse Transcription Kit SuperScriptII (Thermo Fisher Scientific). qPCR was performed with SYBR Green (Fermentas) for detecting the gene expression levels of iNOS. .. All reactions were run in triplicate on an Eppendorf RealPlex Real Time PCR System (Eppendorf) with the standard thermal cycling conditions.

    Expressing:

    Article Title: The effect of memantine, an antagonist of the NMDA glutamate receptor, in in vitro and in vivo infections by Trypanosoma cruzi
    Article Snippet: .. Evaluation of the gene expression of inducible NO synthase in RAW 264.7 macrophages To evaluate the gene expression of inducible nitric oxide synthase, 2 x 106 cells per well were cultured for 18 h in 6-well plates in RPMI medium (10% FCS) in the presence of different concentrations of memantine (ranging from 1 to 100 μM) or none (control) and were stimulated by 10 μg/ml LPS for 18 h. After the incubation time, the supernatant was discarded, and the adhered cells were homogenized with Trizol for RNA extraction (Thermo Fisher Scientific). cDNA was synthetized using the Reverse Transcription Kit SuperScriptII (Thermo Fisher Scientific). qPCR was performed with SYBR Green (Fermentas) for detecting the gene expression levels of iNOS. .. All reactions were run in triplicate on an Eppendorf RealPlex Real Time PCR System (Eppendorf) with the standard thermal cycling conditions.

    Polymerase Chain Reaction:

    Article Title: The effect of memantine, an antagonist of the NMDA glutamate receptor, in in vitro and in vivo infections by Trypanosoma cruzi
    Article Snippet: Evaluation of the gene expression of inducible NO synthase in RAW 264.7 macrophages To evaluate the gene expression of inducible nitric oxide synthase, 2 x 106 cells per well were cultured for 18 h in 6-well plates in RPMI medium (10% FCS) in the presence of different concentrations of memantine (ranging from 1 to 100 μM) or none (control) and were stimulated by 10 μg/ml LPS for 18 h. After the incubation time, the supernatant was discarded, and the adhered cells were homogenized with Trizol for RNA extraction (Thermo Fisher Scientific). cDNA was synthetized using the Reverse Transcription Kit SuperScriptII (Thermo Fisher Scientific). qPCR was performed with SYBR Green (Fermentas) for detecting the gene expression levels of iNOS. .. The threshold cycle (2-ΔΔCt ) method of comparative PCR was used for the data analysis.

    Activated Clotting Time Assay:

    Article Title: The effect of memantine, an antagonist of the NMDA glutamate receptor, in in vitro and in vivo infections by Trypanosoma cruzi
    Article Snippet: Evaluation of the gene expression of inducible NO synthase in RAW 264.7 macrophages To evaluate the gene expression of inducible nitric oxide synthase, 2 x 106 cells per well were cultured for 18 h in 6-well plates in RPMI medium (10% FCS) in the presence of different concentrations of memantine (ranging from 1 to 100 μM) or none (control) and were stimulated by 10 μg/ml LPS for 18 h. After the incubation time, the supernatant was discarded, and the adhered cells were homogenized with Trizol for RNA extraction (Thermo Fisher Scientific). cDNA was synthetized using the Reverse Transcription Kit SuperScriptII (Thermo Fisher Scientific). qPCR was performed with SYBR Green (Fermentas) for detecting the gene expression levels of iNOS. .. The runs were normalized with the ACT-β gene.

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  • 99
    Thermo Fisher vilo superscript iii cdna reverse transcription rt kit
    Expression of Glp1r and Nos1 mRNAs in GnRH neurons harvested by patch electrodes for single-cell qPCR. Real-time qPCR amplification of Gnrh, Gapdh, Glp1r and Nos1 <t>cDNA</t> from GnRH neurons. (A) Expression of Glp1r transcript was detected in two of <t>three</t> pools of GnRH neuronal cytoplasmic samples. The abundance of the Glp1r was low, indicated by its relatively high Ct values (32.5 and 33.1) as compared to the housekeeping gene Gapdh (22.0–22.5). (B) Expression of the Nos1 gene in individually harvested Gfap-negative GnRH neuronal samples. The logarithmic scale of Rn and number of PCR cycles are indicated on the Y and X axes, respectively. The onset of the exponential phase (Ct) is indicated by horizontal lines for each target gene. Column charts are in the inserts to show quantitative results of the qPCR experiments.
    Vilo Superscript Iii Cdna Reverse Transcription Rt Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vilo superscript iii cdna reverse transcription rt kit/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    vilo superscript iii cdna reverse transcription rt kit - by Bioz Stars, 2020-04
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    99
    Thermo Fisher superscript iii reverse transcription kit
    Perturbation of Sm protein homeostasis leads to aggregation in the absence of pICln. (A) Overexpression of SmD3-FLAG in control and pICln-deficient cells analyzed by confocal microscopy by indirect immunofluorescence using antibodies against SMN (green), FLAG (exogenous D3-FLAG; red), or DAPI (blue) upon water (ddH 2 O) or chloroquine treatment. White arrowheads indicate regions shown in insets at the top left corner of each image. (B) Confocal microscopy of indirect immunofluorescence of m 3 G/m 7 G capped RNA (H20 antibody; green), endogenous SmD3 (red), and DAPI (blue) during control or pICln knockdown, upon chloroquine or water treatment. White arrowheads indicate predominant colocalization/staining pattern observed in splicing speckles or Cajal bodies (CBs; control) or disperse higher-order structures (pICln knockdown). (C) Overexpression of SmD1-FLAG in control and pICln-deficient cells analyzed by confocal microscopy. Indirect immunofluorescence using antibodies targeting SMN (green), FLAG (exogenous D1-FLAG; red), and DAPI (blue) upon water (ddH 2 O) or chloroquine treatment. White arrowheads indicate regions shown in insets at the top left corner of each image. (D) Indirect immunofluorescence of endogenous SMN (green) and SmD3 (red) upon treatment of control (i and ii) and pICln-deficient <t>(iii</t> and iv) cells with DMSO solvent or the lysosome inhibitor bafilomycin A. <t>DNA</t> is visualized using DAPI. White arrowheads indicate regions shown in insets at the top left corner of each image. Bars: (A–D, main) 10 µm; (A, C, and D, insets) 1 µm.
    Superscript Iii Reverse Transcription Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 134 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superscript iii reverse transcription kit/product/Thermo Fisher
    Average 99 stars, based on 134 article reviews
    Price from $9.99 to $1999.99
    superscript iii reverse transcription kit - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    93
    Thermo Fisher superscript reverse transcription kit
    Perturbation of Sm protein homeostasis leads to aggregation in the absence of pICln. (A) Overexpression of SmD3-FLAG in control and pICln-deficient cells analyzed by confocal microscopy by indirect immunofluorescence using antibodies against SMN (green), FLAG (exogenous D3-FLAG; red), or DAPI (blue) upon water (ddH 2 O) or chloroquine treatment. White arrowheads indicate regions shown in insets at the top left corner of each image. (B) Confocal microscopy of indirect immunofluorescence of m 3 G/m 7 G capped RNA (H20 antibody; green), endogenous SmD3 (red), and DAPI (blue) during control or pICln knockdown, upon chloroquine or water treatment. White arrowheads indicate predominant colocalization/staining pattern observed in splicing speckles or Cajal bodies (CBs; control) or disperse higher-order structures (pICln knockdown). (C) Overexpression of SmD1-FLAG in control and pICln-deficient cells analyzed by confocal microscopy. Indirect immunofluorescence using antibodies targeting SMN (green), FLAG (exogenous D1-FLAG; red), and DAPI (blue) upon water (ddH 2 O) or chloroquine treatment. White arrowheads indicate regions shown in insets at the top left corner of each image. (D) Indirect immunofluorescence of endogenous SMN (green) and SmD3 (red) upon treatment of control (i and ii) and pICln-deficient <t>(iii</t> and iv) cells with DMSO solvent or the lysosome inhibitor bafilomycin A. <t>DNA</t> is visualized using DAPI. White arrowheads indicate regions shown in insets at the top left corner of each image. Bars: (A–D, main) 10 µm; (A, C, and D, insets) 1 µm.
    Superscript Reverse Transcription Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superscript reverse transcription kit/product/Thermo Fisher
    Average 93 stars, based on 18 article reviews
    Price from $9.99 to $1999.99
    superscript reverse transcription kit - by Bioz Stars, 2020-04
    93/100 stars
      Buy from Supplier

    Image Search Results


    Expression of Glp1r and Nos1 mRNAs in GnRH neurons harvested by patch electrodes for single-cell qPCR. Real-time qPCR amplification of Gnrh, Gapdh, Glp1r and Nos1 cDNA from GnRH neurons. (A) Expression of Glp1r transcript was detected in two of three pools of GnRH neuronal cytoplasmic samples. The abundance of the Glp1r was low, indicated by its relatively high Ct values (32.5 and 33.1) as compared to the housekeeping gene Gapdh (22.0–22.5). (B) Expression of the Nos1 gene in individually harvested Gfap-negative GnRH neuronal samples. The logarithmic scale of Rn and number of PCR cycles are indicated on the Y and X axes, respectively. The onset of the exponential phase (Ct) is indicated by horizontal lines for each target gene. Column charts are in the inserts to show quantitative results of the qPCR experiments.

    Journal: Frontiers in Cellular Neuroscience

    Article Title: Glucagon-Like Peptide-1 Excites Firing and Increases GABAergic Miniature Postsynaptic Currents (mPSCs) in Gonadotropin-Releasing Hormone (GnRH) Neurons of the Male Mice via Activation of Nitric Oxide (NO) and Suppression of Endocannabinoid Signaling Pathways

    doi: 10.3389/fncel.2016.00214

    Figure Lengend Snippet: Expression of Glp1r and Nos1 mRNAs in GnRH neurons harvested by patch electrodes for single-cell qPCR. Real-time qPCR amplification of Gnrh, Gapdh, Glp1r and Nos1 cDNA from GnRH neurons. (A) Expression of Glp1r transcript was detected in two of three pools of GnRH neuronal cytoplasmic samples. The abundance of the Glp1r was low, indicated by its relatively high Ct values (32.5 and 33.1) as compared to the housekeeping gene Gapdh (22.0–22.5). (B) Expression of the Nos1 gene in individually harvested Gfap-negative GnRH neuronal samples. The logarithmic scale of Rn and number of PCR cycles are indicated on the Y and X axes, respectively. The onset of the exponential phase (Ct) is indicated by horizontal lines for each target gene. Column charts are in the inserts to show quantitative results of the qPCR experiments.

    Article Snippet: The collected cytoplasm was reverse transcribed directly in 20 μl reactions using the ViLO SuperScript III cDNA reverse transcription (RT) kit (Thermo Fisher Scientific, Waltham, MA, USA).

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Amplification, Polymerase Chain Reaction

    Perturbation of Sm protein homeostasis leads to aggregation in the absence of pICln. (A) Overexpression of SmD3-FLAG in control and pICln-deficient cells analyzed by confocal microscopy by indirect immunofluorescence using antibodies against SMN (green), FLAG (exogenous D3-FLAG; red), or DAPI (blue) upon water (ddH 2 O) or chloroquine treatment. White arrowheads indicate regions shown in insets at the top left corner of each image. (B) Confocal microscopy of indirect immunofluorescence of m 3 G/m 7 G capped RNA (H20 antibody; green), endogenous SmD3 (red), and DAPI (blue) during control or pICln knockdown, upon chloroquine or water treatment. White arrowheads indicate predominant colocalization/staining pattern observed in splicing speckles or Cajal bodies (CBs; control) or disperse higher-order structures (pICln knockdown). (C) Overexpression of SmD1-FLAG in control and pICln-deficient cells analyzed by confocal microscopy. Indirect immunofluorescence using antibodies targeting SMN (green), FLAG (exogenous D1-FLAG; red), and DAPI (blue) upon water (ddH 2 O) or chloroquine treatment. White arrowheads indicate regions shown in insets at the top left corner of each image. (D) Indirect immunofluorescence of endogenous SMN (green) and SmD3 (red) upon treatment of control (i and ii) and pICln-deficient (iii and iv) cells with DMSO solvent or the lysosome inhibitor bafilomycin A. DNA is visualized using DAPI. White arrowheads indicate regions shown in insets at the top left corner of each image. Bars: (A–D, main) 10 µm; (A, C, and D, insets) 1 µm.

    Journal: The Journal of Cell Biology

    Article Title: Impaired spliceosomal UsnRNP assembly leads to Sm mRNA down-regulation and Sm protein degradation

    doi: 10.1083/jcb.201611108

    Figure Lengend Snippet: Perturbation of Sm protein homeostasis leads to aggregation in the absence of pICln. (A) Overexpression of SmD3-FLAG in control and pICln-deficient cells analyzed by confocal microscopy by indirect immunofluorescence using antibodies against SMN (green), FLAG (exogenous D3-FLAG; red), or DAPI (blue) upon water (ddH 2 O) or chloroquine treatment. White arrowheads indicate regions shown in insets at the top left corner of each image. (B) Confocal microscopy of indirect immunofluorescence of m 3 G/m 7 G capped RNA (H20 antibody; green), endogenous SmD3 (red), and DAPI (blue) during control or pICln knockdown, upon chloroquine or water treatment. White arrowheads indicate predominant colocalization/staining pattern observed in splicing speckles or Cajal bodies (CBs; control) or disperse higher-order structures (pICln knockdown). (C) Overexpression of SmD1-FLAG in control and pICln-deficient cells analyzed by confocal microscopy. Indirect immunofluorescence using antibodies targeting SMN (green), FLAG (exogenous D1-FLAG; red), and DAPI (blue) upon water (ddH 2 O) or chloroquine treatment. White arrowheads indicate regions shown in insets at the top left corner of each image. (D) Indirect immunofluorescence of endogenous SMN (green) and SmD3 (red) upon treatment of control (i and ii) and pICln-deficient (iii and iv) cells with DMSO solvent or the lysosome inhibitor bafilomycin A. DNA is visualized using DAPI. White arrowheads indicate regions shown in insets at the top left corner of each image. Bars: (A–D, main) 10 µm; (A, C, and D, insets) 1 µm.

    Article Snippet: The precipitated RNA was treated with DNase using Turbo DNA-free kit (AM1907; Thermo Fisher Scientific) and reverse transcribed using SuperScript III reverse transcription kit (18080-093; Thermo Fisher Scientific) using random hexamer primers.

    Techniques: Over Expression, Confocal Microscopy, Immunofluorescence, Staining