reverse transcriptase pcr qrt pcr kit (Thermo Fisher)
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Name:
SuperScript III First Strand Synthesis SuperMix for qRT PCR
Description:
SuperScript III First Strand Synthesis SuperMix for qRT PCR provides the high temperature capability of SuperScript III Reverse Transcriptase in an optimized SuperMix format for the synthesis of first strand cDNA for use in real time quantitative RT PCR qRT PCR The simple time saving reaction set up uses just two tubes a 2X Reaction Mix and an Enzyme Mix Enzyme mixSuperScript III Reverse Transcriptase included in the RT Enzyme Mix is a version of M MLV RT that has been engineered to reduce RNase H activity and provide increased thermal stability The enzyme can be used to synthesize cDNA at a temperature range of 42 60°C providing increased specificity higher yields of cDNA and more full length product than other reverse transcriptases Because SuperScript III RT is not significantly inhibited by ribosomal and transfer RNA it can be used to synthesize cDNA from total RNA RNaseOUT Recombinant Ribonuclease Inhibitor also included in the enzyme mix is an RNase inhibitor protein that safeguards against the degradation of target RNA due to ribonuclease contamination of the RNA preparation Reaction mixThe 2X RT Reaction Mix includes oligo dT 20 random hexamers MgCl2 and dNTPs in a buffer formulation that has been optimized for qRT PCR E coli RNase H is provided as a separate tube in the kit to remove the RNA template from the cDNA RNA hybrid molecule after first strand synthesis This has been shown to increase sensitivity in qRT PCR Using SuperScript III First Strand Synthesis SuperMixThis SuperMix formulation can be used to quantify fewer than 10 copies of a target gene in qRT PCR with a broad dynamic range that supports accurate quantification of high copy mRNA from up to 1 µg of total RNA Reagents are provided for 50 or 250 RT reactions of 20 µL each
Catalog Number:
11752050
Price:
None
Category:
Kits and Assays
Applications:
PCR & Real-Time PCR|Reverse Transcription
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Structured Review
Thermo Fisher
reverse transcriptase pcr qrt pcr kit
SuperScript III First Strand Synthesis SuperMix for qRT PCR provides the high temperature capability of SuperScript III Reverse Transcriptase in an optimized SuperMix format for the synthesis of first strand cDNA for use in real time quantitative RT PCR qRT PCR The simple time saving reaction set up uses just two tubes a 2X Reaction Mix and an Enzyme Mix Enzyme mixSuperScript III Reverse Transcriptase included in the RT Enzyme Mix is a version of M MLV RT that has been engineered to reduce RNase H activity and provide increased thermal stability The enzyme can be used to synthesize cDNA at a temperature range of 42 60°C providing increased specificity higher yields of cDNA and more full length product than other reverse transcriptases Because SuperScript III RT is not significantly inhibited by ribosomal and transfer RNA it can be used to synthesize cDNA from total RNA RNaseOUT Recombinant Ribonuclease Inhibitor also included in the enzyme mix is an RNase inhibitor protein that safeguards against the degradation of target RNA due to ribonuclease contamination of the RNA preparation Reaction mixThe 2X RT Reaction Mix includes oligo dT 20 random hexamers MgCl2 and dNTPs in a buffer formulation that has been optimized for qRT PCR E coli RNase H is provided as a separate tube in the kit to remove the RNA template from the cDNA RNA hybrid molecule after first strand synthesis This has been shown to increase sensitivity in qRT PCR Using SuperScript III First Strand Synthesis SuperMixThis SuperMix formulation can be used to quantify fewer than 10 copies of a target gene in qRT PCR with a broad dynamic range that supports accurate quantification of high copy mRNA from up to 1 µg of total RNA Reagents are provided for 50 or 250 RT reactions of 20 µL each
https://www.bioz.com/result/reverse transcriptase pcr qrt pcr kit/product/Thermo Fisher
Average 99 stars, based on 1 article reviews
Price from $9.99 to $1999.99
SuperScript III First Strand Synthesis SuperMix for qRT PCR provides the high temperature capability of SuperScript III Reverse Transcriptase in an optimized SuperMix format for the synthesis of first strand cDNA for use in real time quantitative RT PCR qRT PCR The simple time saving reaction set up uses just two tubes a 2X Reaction Mix and an Enzyme Mix Enzyme mixSuperScript III Reverse Transcriptase included in the RT Enzyme Mix is a version of M MLV RT that has been engineered to reduce RNase H activity and provide increased thermal stability The enzyme can be used to synthesize cDNA at a temperature range of 42 60°C providing increased specificity higher yields of cDNA and more full length product than other reverse transcriptases Because SuperScript III RT is not significantly inhibited by ribosomal and transfer RNA it can be used to synthesize cDNA from total RNA RNaseOUT Recombinant Ribonuclease Inhibitor also included in the enzyme mix is an RNase inhibitor protein that safeguards against the degradation of target RNA due to ribonuclease contamination of the RNA preparation Reaction mixThe 2X RT Reaction Mix includes oligo dT 20 random hexamers MgCl2 and dNTPs in a buffer formulation that has been optimized for qRT PCR E coli RNase H is provided as a separate tube in the kit to remove the RNA template from the cDNA RNA hybrid molecule after first strand synthesis This has been shown to increase sensitivity in qRT PCR Using SuperScript III First Strand Synthesis SuperMixThis SuperMix formulation can be used to quantify fewer than 10 copies of a target gene in qRT PCR with a broad dynamic range that supports accurate quantification of high copy mRNA from up to 1 µg of total RNA Reagents are provided for 50 or 250 RT reactions of 20 µL each
https://www.bioz.com/result/reverse transcriptase pcr qrt pcr kit/product/Thermo Fisher
Average 99 stars, based on 1 article reviews
Price from $9.99 to $1999.99
reverse transcriptase pcr qrt pcr kit - by Bioz Stars,
2021-04
99/100 stars
Images
1) Product Images from "Increased Bacterial Load and Expression of Antimicrobial Peptides in Skin of Barrier-Deficient Mice with Reduced Cancer Susceptibility"
Article Title: Increased Bacterial Load and Expression of Antimicrobial Peptides in Skin of Barrier-Deficient Mice with Reduced Cancer Susceptibility
Journal: The Journal of Investigative Dermatology
doi: 10.1038/JID.2015.383
Figure Legend Snippet: Microbiota independent inflammatory and stress response phenotypes of EPI-/- skin. ( a ) qPCR of 16s RNA gene in WT, EPI-/-, antibiotic-treated EPI-/-, and flora-deficient EPI-/- ear skin samples. ( b ) Number of CD4 + CD3 + cells per mm 2 dermis. ( c ) Number of γδTCR + cells per mm epidermis. ( d ) Number of Vγ3 + cells per mm epidermis. ( e ) qRT-PCR of Rae-1 in WT, EPI-/-, antibiotic-treated EPI-/-, and flora-deficient EPI-/- epidermis. ( f ) Quantification of MPO in WT, EPI-/-, and flora-deficient skin lysates. Data are means ± SEM from at least four ( a ) or three ( b–f ) mice per group. * P
Techniques Used: Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Mouse Assay
Figure Legend Snippet: AMP expression and protease activity in EPI-/- epidermis after microbiota modulation. ( a–k ) qRT-PCR of Camp ( a ) , Defb1 ( b ), Defb2 ( c ), Defb3 ( d ), Defb6 ( e ), S100A9 ( f ), Pla2g2a ( g ), Reg3b ( h ), Reg3g ( i ), and Serpina1b ( j ) in WT, EPI-/-, antibiotic-treated EPI-/-, and flora-deficient EPI-/- epidermis. Data are means ± SEM from at least four mice per group. * P
Techniques Used: Expressing, Activity Assay, Quantitative RT-PCR, Mouse Assay
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