reverse phase hplc  (Thermo Fisher)


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    Name:
    Human Reverse Primer HPLC
    Description:
    Use our tool to find and order pre designed primers for PCR and Sanger sequencing Free of SNPs and dimers target specific and suitable for universal PCR conditions these primers can be ordered unmodified M13 tailed HPLC purified or desalted All primers are checked by mass spectrometry
    Catalog Number:
    a15632
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    None
    Applications:
    Kits & Reagents for Sanger Sequencing|Sanger Sequencing|Sanger Sequencing Technology & Accessories|Sequencing
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    Oligos Primers Probes Nucleotides
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    Structured Review

    Thermo Fisher reverse phase hplc
    Biotin labeling of duplex ODN I·II with aziridine cofactor 5 and M.HhaI. ( a ) Anion-exchange <t>HPLC</t> analysis of the coupling reaction between the aziridine cofactor 5 and the duplex I·II by M.HhaI. (Trace 1) Analysis directly after mixing the components; (Trace 2) analysis after 3 h of incubation at 37 °C; (Trace 3) analysis after 3 h of incubation at 37 °C followed by the release of the modified duplex I 5 ·II from the <t>protein-DNA</t> complex by heat denaturation; (Trace 4) analysis of streptavidin binding to I 5 ·II ; ( b ) Enzymatic fragmentation of the product duplex I 5 ·II with DNase I, phosphodiesterase from Crotalus adamanteus , phosphodiesterase from calf spleen and alkaline phosphatase analyzed by reverse-phase HPLC. The modified nucleoside (dC 5 ) elutes after 2’-deoxycytidine (dC), C 5-methyl-2’-deoxycytidine (dC Me ), 2’-deoxyinosine (dI), 2’-deoxyguanosine (dG) and 2’-deoxythymidine (dT) (2’-deoxyadenosinewas converted to dI by contaminating adenosine deaminase activity during the fragmentation reaction).
    Use our tool to find and order pre designed primers for PCR and Sanger sequencing Free of SNPs and dimers target specific and suitable for universal PCR conditions these primers can be ordered unmodified M13 tailed HPLC purified or desalted All primers are checked by mass spectrometry
    https://www.bioz.com/result/reverse phase hplc/product/Thermo Fisher
    Average 98 stars, based on 180 article reviews
    Price from $9.99 to $1999.99
    reverse phase hplc - by Bioz Stars, 2020-11
    98/100 stars

    Images

    1) Product Images from "A 7-Deazaadenosylaziridine Cofactor for Sequence-Specific Labeling of DNA by the DNA Cytosine-C5 Methyltransferase M.HhaI"

    Article Title: A 7-Deazaadenosylaziridine Cofactor for Sequence-Specific Labeling of DNA by the DNA Cytosine-C5 Methyltransferase M.HhaI

    Journal: Molecules

    doi: 10.3390/molecules201119723

    Biotin labeling of duplex ODN I·II with aziridine cofactor 5 and M.HhaI. ( a ) Anion-exchange HPLC analysis of the coupling reaction between the aziridine cofactor 5 and the duplex I·II by M.HhaI. (Trace 1) Analysis directly after mixing the components; (Trace 2) analysis after 3 h of incubation at 37 °C; (Trace 3) analysis after 3 h of incubation at 37 °C followed by the release of the modified duplex I 5 ·II from the protein-DNA complex by heat denaturation; (Trace 4) analysis of streptavidin binding to I 5 ·II ; ( b ) Enzymatic fragmentation of the product duplex I 5 ·II with DNase I, phosphodiesterase from Crotalus adamanteus , phosphodiesterase from calf spleen and alkaline phosphatase analyzed by reverse-phase HPLC. The modified nucleoside (dC 5 ) elutes after 2’-deoxycytidine (dC), C 5-methyl-2’-deoxycytidine (dC Me ), 2’-deoxyinosine (dI), 2’-deoxyguanosine (dG) and 2’-deoxythymidine (dT) (2’-deoxyadenosinewas converted to dI by contaminating adenosine deaminase activity during the fragmentation reaction).
    Figure Legend Snippet: Biotin labeling of duplex ODN I·II with aziridine cofactor 5 and M.HhaI. ( a ) Anion-exchange HPLC analysis of the coupling reaction between the aziridine cofactor 5 and the duplex I·II by M.HhaI. (Trace 1) Analysis directly after mixing the components; (Trace 2) analysis after 3 h of incubation at 37 °C; (Trace 3) analysis after 3 h of incubation at 37 °C followed by the release of the modified duplex I 5 ·II from the protein-DNA complex by heat denaturation; (Trace 4) analysis of streptavidin binding to I 5 ·II ; ( b ) Enzymatic fragmentation of the product duplex I 5 ·II with DNase I, phosphodiesterase from Crotalus adamanteus , phosphodiesterase from calf spleen and alkaline phosphatase analyzed by reverse-phase HPLC. The modified nucleoside (dC 5 ) elutes after 2’-deoxycytidine (dC), C 5-methyl-2’-deoxycytidine (dC Me ), 2’-deoxyinosine (dI), 2’-deoxyguanosine (dG) and 2’-deoxythymidine (dT) (2’-deoxyadenosinewas converted to dI by contaminating adenosine deaminase activity during the fragmentation reaction).

    Techniques Used: Labeling, High Performance Liquid Chromatography, Incubation, Modification, Binding Assay, Activity Assay

    2) Product Images from "Numerical analysis of intracellular amino acid profiles of breast cancer cells with K-Ras or PI3K mutation in response to kinase inhibitors"

    Article Title: Numerical analysis of intracellular amino acid profiles of breast cancer cells with K-Ras or PI3K mutation in response to kinase inhibitors

    Journal: BMC Cancer

    doi: 10.1186/s12885-018-4972-7

    Overview of profiling free amino acids in cells. a Treatment of inhibitors to cells. The three types of isogenic MCF-10A cells including WT, K-Ras, and K-Ras/PI3K were treated for 24 or 48 h with three different kinase inhibitors: multiple kinase inhibitor (REGO), PI3K inhibitor (PI3K-i), and MEK inhibitor (MEK-i). Non-treated (NT) cells were used as controls. b Quantification of amino aicd levels. The IFAAs were extracted, chemically labeled, and separated to generate the amino acid profile in triplicate for 24 different conditions. The qunatified AA levels from each profile were represented into a 19-by-1 vector for the subsequent analysis. Note that unassigned peaks in an HPLC chromatogram were marked with *
    Figure Legend Snippet: Overview of profiling free amino acids in cells. a Treatment of inhibitors to cells. The three types of isogenic MCF-10A cells including WT, K-Ras, and K-Ras/PI3K were treated for 24 or 48 h with three different kinase inhibitors: multiple kinase inhibitor (REGO), PI3K inhibitor (PI3K-i), and MEK inhibitor (MEK-i). Non-treated (NT) cells were used as controls. b Quantification of amino aicd levels. The IFAAs were extracted, chemically labeled, and separated to generate the amino acid profile in triplicate for 24 different conditions. The qunatified AA levels from each profile were represented into a 19-by-1 vector for the subsequent analysis. Note that unassigned peaks in an HPLC chromatogram were marked with *

    Techniques Used: Labeling, Plasmid Preparation, High Performance Liquid Chromatography

    3) Product Images from "Numerical analysis of intracellular amino acid profiles of breast cancer cells with K-Ras or PI3K mutation in response to kinase inhibitors"

    Article Title: Numerical analysis of intracellular amino acid profiles of breast cancer cells with K-Ras or PI3K mutation in response to kinase inhibitors

    Journal: BMC Cancer

    doi: 10.1186/s12885-018-4972-7

    Overview of profiling free amino acids in cells. a Treatment of inhibitors to cells. The three types of isogenic MCF-10A cells including WT, K-Ras, and K-Ras/PI3K were treated for 24 or 48 h with three different kinase inhibitors: multiple kinase inhibitor (REGO), PI3K inhibitor (PI3K-i), and MEK inhibitor (MEK-i). Non-treated (NT) cells were used as controls. b Quantification of amino aicd levels. The IFAAs were extracted, chemically labeled, and separated to generate the amino acid profile in triplicate for 24 different conditions. The qunatified AA levels from each profile were represented into a 19-by-1 vector for the subsequent analysis. Note that unassigned peaks in an HPLC chromatogram were marked with *
    Figure Legend Snippet: Overview of profiling free amino acids in cells. a Treatment of inhibitors to cells. The three types of isogenic MCF-10A cells including WT, K-Ras, and K-Ras/PI3K were treated for 24 or 48 h with three different kinase inhibitors: multiple kinase inhibitor (REGO), PI3K inhibitor (PI3K-i), and MEK inhibitor (MEK-i). Non-treated (NT) cells were used as controls. b Quantification of amino aicd levels. The IFAAs were extracted, chemically labeled, and separated to generate the amino acid profile in triplicate for 24 different conditions. The qunatified AA levels from each profile were represented into a 19-by-1 vector for the subsequent analysis. Note that unassigned peaks in an HPLC chromatogram were marked with *

    Techniques Used: Labeling, Plasmid Preparation, High Performance Liquid Chromatography

    4) Product Images from "Identification of Naturally Processed Hepatitis C Virus-Derived Major Histocompatibility Complex Class I Ligands"

    Article Title: Identification of Naturally Processed Hepatitis C Virus-Derived Major Histocompatibility Complex Class I Ligands

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0029286

    Identification of naturally processed HLA-A2 ligands. Following large-scale expansion, cell pellets were lysed and MHC class I molecules with bound ligands were purified by immunoprecipitation. Subsequently, ligands were eluted and separated by high performance liquid chromatography (HPLC). Fractions of interest were sequenced by mass spectrometry.
    Figure Legend Snippet: Identification of naturally processed HLA-A2 ligands. Following large-scale expansion, cell pellets were lysed and MHC class I molecules with bound ligands were purified by immunoprecipitation. Subsequently, ligands were eluted and separated by high performance liquid chromatography (HPLC). Fractions of interest were sequenced by mass spectrometry.

    Techniques Used: Purification, Immunoprecipitation, High Performance Liquid Chromatography, Mass Spectrometry

    Related Articles

    High Performance Liquid Chromatography:

    Article Title: Exogenous Nitric Oxide Enhances Disease Resistance by Nitrosylation and Inhibition of S-Nitrosoglutathione Reductase in Peach Fruit
    Article Snippet: .. HPLC Fractionation Fractionation of the samples was carried out by high pH reverse-phase HPLC (EASY-nLC 1000, Thermo Fisher Scientific, Waltham, MA, United States) applying 300 Extend C18 column (5 μM particles, 4.6 mM ID, and 250 mM length; Agilent Technologies, Santa Clara, CA, United States). .. First, the peptides were dissociated into 80 fractions with a gradient of 2–60% acetonitrile (ACN) in 10 mM NH4 HCO3 (pH 10) over 80 min. Then, the peptides were combined into four fractions and vacuum dried.

    Article Title: Citrus aurantium and Rhodiola rosea in combination reduce visceral white adipose tissue and increase hypothalamic norepinephrine in a rat model of diet-induced obesity
    Article Snippet: .. Blood plasma samples and brain sections were analyzed by reverse-phase HPLC (Dionex Ultimate 3000, Thermofisher, Sunnyvale, CA, USA) with electrochemical detection (Coulochem III, Thermofisher, Sunnyvale, CA, USA). .. An acetonitrile-based phosphate buffer mobile phase (MD-TM; Dionex, Thermofisher, Sunnnyvale, CA, USA) was used for all experiments.

    Article Title: Reaction of Hydrogen Sulfide with Disulfide and Sulfenic Acid to Form the Strongly Nucleophilic Persulfide *Reaction of Hydrogen Sulfide with Disulfide and Sulfenic Acid to Form the Strongly Nucleophilic Persulfide * ♦
    Article Snippet: .. Reverse phase HPLC (Dionex, Thermo Fisher Scientific) was performed using a C18 column and a combination of water (solvent A) and acetonitrile (solvent B), both containing 0.5% formic acid. ..

    Article Title: Structural characterization by transmission electron microscopy and immunoreactivity of recombinant Hendra virus nucleocapsid protein expressed and purified from Escherichia coli.
    Article Snippet: .. Mass spectrometry Purified HeV N FL and HeV N CORE were desalted by reverse-phase HPLC (Dionex UltiMate 3000 HPLC) using a C18 Jupiter column (Phenomenex), and the proteins eluted directly onto a microTOF-QII electrospray ionization mass spectrometer (Bruker) using a gradient of acetonitrile containing 0.1% formic acid. ..

    Article Title: APEH Inhibition Affects Osteosarcoma Cell Viability via Downregulation of the Proteasome
    Article Snippet: .. The eluate recovered after each filtration (0.18 mL) was analyzed by reverse-phase HPLC (BioLC; Dionex, Thermo Scientific, USA) on a µBondapak C18 column (300 × 3.9 mm ID; Waters, Milford, MA, USA) eluted with a linear gradient of acetonitrile 0.1% TFA from 5% to 95% in 37 min at a flow rate of 1 mL/min. ..

    Article Title: Different Covalent Immobilizations Modulate Lipase Activities of Hypocrea pseudokoningii
    Article Snippet: .. At different times, the degree of hydrolysis was measured by reverse-phase HPLC (Spectra Physics SP 100 coupled with a Spectra Physics SP 8450UV detector) on a Kromasil C18 (25 cm × 0.4 cm) column supplied by Analysis Vinicos (Tomelloso, Spain) using the corresponding mobile phase and flow rates listed in below. ..

    Article Title: Structural characterization by transmission electron microscopy and immunoreactivity of recombinant Hendra virus nucleocapsid protein expressed and purified from Escherichia coli
    Article Snippet: .. 2.6 Mass spectrometry Purified HeV NFL and HeV NCORE were desalted by reverse-phase HPLC (Dionex UltiMate 3000 HPLC) using a C18 Jupiter column (Phenomenex), and the proteins eluted directly onto a microTOF-QII electrospray ionization mass spectrometer (Bruker) using a gradient of acetonitrile containing 0.1% formic acid. ..

    Article Title: A Novel, Stable, Aqueous Glucagon Formulation Using Ferulic Acid as an Excipient
    Article Snippet: .. Glucagon degradation was analyzed by reverse-phase HPLC (Surveyor System, Thermo Fisher Scientific) using the method outlined by the United States Pharmacopeial Convention. ..

    Flow Cytometry:

    Article Title: APEH Inhibition Affects Osteosarcoma Cell Viability via Downregulation of the Proteasome
    Article Snippet: .. The eluate recovered after each filtration (0.18 mL) was analyzed by reverse-phase HPLC (BioLC; Dionex, Thermo Scientific, USA) on a µBondapak C18 column (300 × 3.9 mm ID; Waters, Milford, MA, USA) eluted with a linear gradient of acetonitrile 0.1% TFA from 5% to 95% in 37 min at a flow rate of 1 mL/min. ..

    Article Title: Different Covalent Immobilizations Modulate Lipase Activities of Hypocrea pseudokoningii
    Article Snippet: .. At different times, the degree of hydrolysis was measured by reverse-phase HPLC (Spectra Physics SP 100 coupled with a Spectra Physics SP 8450UV detector) on a Kromasil C18 (25 cm × 0.4 cm) column supplied by Analysis Vinicos (Tomelloso, Spain) using the corresponding mobile phase and flow rates listed in below. ..

    Filtration:

    Article Title: APEH Inhibition Affects Osteosarcoma Cell Viability via Downregulation of the Proteasome
    Article Snippet: .. The eluate recovered after each filtration (0.18 mL) was analyzed by reverse-phase HPLC (BioLC; Dionex, Thermo Scientific, USA) on a µBondapak C18 column (300 × 3.9 mm ID; Waters, Milford, MA, USA) eluted with a linear gradient of acetonitrile 0.1% TFA from 5% to 95% in 37 min at a flow rate of 1 mL/min. ..

    Purification:

    Article Title: Structural characterization by transmission electron microscopy and immunoreactivity of recombinant Hendra virus nucleocapsid protein expressed and purified from Escherichia coli.
    Article Snippet: .. Mass spectrometry Purified HeV N FL and HeV N CORE were desalted by reverse-phase HPLC (Dionex UltiMate 3000 HPLC) using a C18 Jupiter column (Phenomenex), and the proteins eluted directly onto a microTOF-QII electrospray ionization mass spectrometer (Bruker) using a gradient of acetonitrile containing 0.1% formic acid. ..

    Article Title: Structural characterization by transmission electron microscopy and immunoreactivity of recombinant Hendra virus nucleocapsid protein expressed and purified from Escherichia coli
    Article Snippet: .. 2.6 Mass spectrometry Purified HeV NFL and HeV NCORE were desalted by reverse-phase HPLC (Dionex UltiMate 3000 HPLC) using a C18 Jupiter column (Phenomenex), and the proteins eluted directly onto a microTOF-QII electrospray ionization mass spectrometer (Bruker) using a gradient of acetonitrile containing 0.1% formic acid. ..

    Fractionation:

    Article Title: Exogenous Nitric Oxide Enhances Disease Resistance by Nitrosylation and Inhibition of S-Nitrosoglutathione Reductase in Peach Fruit
    Article Snippet: .. HPLC Fractionation Fractionation of the samples was carried out by high pH reverse-phase HPLC (EASY-nLC 1000, Thermo Fisher Scientific, Waltham, MA, United States) applying 300 Extend C18 column (5 μM particles, 4.6 mM ID, and 250 mM length; Agilent Technologies, Santa Clara, CA, United States). .. First, the peptides were dissociated into 80 fractions with a gradient of 2–60% acetonitrile (ACN) in 10 mM NH4 HCO3 (pH 10) over 80 min. Then, the peptides were combined into four fractions and vacuum dried.

    Mass Spectrometry:

    Article Title: Structural characterization by transmission electron microscopy and immunoreactivity of recombinant Hendra virus nucleocapsid protein expressed and purified from Escherichia coli.
    Article Snippet: .. Mass spectrometry Purified HeV N FL and HeV N CORE were desalted by reverse-phase HPLC (Dionex UltiMate 3000 HPLC) using a C18 Jupiter column (Phenomenex), and the proteins eluted directly onto a microTOF-QII electrospray ionization mass spectrometer (Bruker) using a gradient of acetonitrile containing 0.1% formic acid. ..

    Article Title: Structural characterization by transmission electron microscopy and immunoreactivity of recombinant Hendra virus nucleocapsid protein expressed and purified from Escherichia coli
    Article Snippet: .. 2.6 Mass spectrometry Purified HeV NFL and HeV NCORE were desalted by reverse-phase HPLC (Dionex UltiMate 3000 HPLC) using a C18 Jupiter column (Phenomenex), and the proteins eluted directly onto a microTOF-QII electrospray ionization mass spectrometer (Bruker) using a gradient of acetonitrile containing 0.1% formic acid. ..

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  • 99
    Thermo Fisher betasil c18 rp hplc column
    <t>HPLC-MS</t> analysis of cowpea extracts for destruxin (DTX) production. (A) Analysis of not colonized (free of fungus) plants (negative control); (B) plants endophytically colonized by Metarhizium robertsii ARSEF 2575; and (C) not-colonized plants spiked with DTX standards (positive control). The cowpea seeds, both fungus-inoculated and control (not colonized) were incubated on moist filter paper under optimal light (16L∶8D) and temperature (25°C) conditions for 12 days at which time the germlings had developed roots, stems, cotyledons and two true leaves. DTXs were extracted from entire plants using methanol 100% and <t>SPE-C18</t> cartridges.
    Betasil C18 Rp Hplc Column, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/betasil c18 rp hplc column/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    betasil c18 rp hplc column - by Bioz Stars, 2020-11
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    94
    Thermo Fisher rp hplc
    Identification and purification of disulphide-bonded peptides from a tryptic digest of Pf332 DBL domain. A. Comparison of the analytical <t>RP-HPLC</t> elution profiles for DTT-reduced and non-reduced tryptic digest of refolded Pf332 DBL domain. Reduced (upper trace) and non-reduced (lower trace) digests (20 μg) were fractionated on a Vydac <t>C18</t> (4.6 mm inner diameter × 250 mm) column under identical chromatographic conditions. The column was developed at a flow rate of 1 ml min −1 with a linear 120 min gradient from 0 to 70% buffer B, where buffer A was 0.05% (v/v) trifluoroacetic acid in Milli Q water, and buffer B was 0.05% (v/v) trifluoroacetic acid in acetonitrile. Asterisk-labelled peaks within the non-reduced chromatogram represent those altered by DTT reduction. B. Preparative scale RP-HPLC purification of peptides from the tryptic digest of Pf332 DBL domain. A total of 125 μg of digest was fractionated by elution from a Vydac C18 column (4.6 mm inner diameter × 250 mm) column using the elution conditions described in (A). Peptide fractions found to contain disulphide-bonded peptides by Edman degradation and mass spectrometry are indicated by peak numbers and correspond to those given in Table 1 (see Experimental procedures for further details).
    Rp Hplc, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 133 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rp hplc/product/Thermo Fisher
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    91
    Thermo Fisher high performance liquid chromatography hplc analysis reverse phase hplc
    <t>RP-HPLC</t> Analysis of Propolis from T. Sirindhornae (column: 250 mm X 4.6 mm, 5 μM,λ 250 nm). Standard compounds: caffeic acid (1), apigenin (2), p-coumaric acid (3), and pinocembrin (4) (A). Component analysis of <t>DMEP-A</t> (B), DMEP-B (C) and DMEP-C (D) are indicated by the black lines.
    High Performance Liquid Chromatography Hplc Analysis Reverse Phase Hplc, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/high performance liquid chromatography hplc analysis reverse phase hplc/product/Thermo Fisher
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    high performance liquid chromatography hplc analysis reverse phase hplc - by Bioz Stars, 2020-11
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    Image Search Results


    HPLC-MS analysis of cowpea extracts for destruxin (DTX) production. (A) Analysis of not colonized (free of fungus) plants (negative control); (B) plants endophytically colonized by Metarhizium robertsii ARSEF 2575; and (C) not-colonized plants spiked with DTX standards (positive control). The cowpea seeds, both fungus-inoculated and control (not colonized) were incubated on moist filter paper under optimal light (16L∶8D) and temperature (25°C) conditions for 12 days at which time the germlings had developed roots, stems, cotyledons and two true leaves. DTXs were extracted from entire plants using methanol 100% and SPE-C18 cartridges.

    Journal: PLoS ONE

    Article Title: Production of Destruxins from Metarhizium spp. Fungi in Artificial Medium and in Endophytically Colonized Cowpea Plants

    doi: 10.1371/journal.pone.0104946

    Figure Lengend Snippet: HPLC-MS analysis of cowpea extracts for destruxin (DTX) production. (A) Analysis of not colonized (free of fungus) plants (negative control); (B) plants endophytically colonized by Metarhizium robertsii ARSEF 2575; and (C) not-colonized plants spiked with DTX standards (positive control). The cowpea seeds, both fungus-inoculated and control (not colonized) were incubated on moist filter paper under optimal light (16L∶8D) and temperature (25°C) conditions for 12 days at which time the germlings had developed roots, stems, cotyledons and two true leaves. DTXs were extracted from entire plants using methanol 100% and SPE-C18 cartridges.

    Article Snippet: The LC-MS system consisted of a Betasil C18 RP HPLC column (100×2.1 mm, Thermo Fisher), coupled to a Surveyor MS Pump Plus, a Surveyor Auto Sampler Plus and a PDA UV–vis absorbance detector in-line with an LCQ Advantage Max mass spectrometer and electrospray (esi) ionization source (Thermo Electron Corp, San Jose, CA, USA).

    Techniques: High Performance Liquid Chromatography, Mass Spectrometry, Negative Control, Positive Control, Incubation

    Identification and purification of disulphide-bonded peptides from a tryptic digest of Pf332 DBL domain. A. Comparison of the analytical RP-HPLC elution profiles for DTT-reduced and non-reduced tryptic digest of refolded Pf332 DBL domain. Reduced (upper trace) and non-reduced (lower trace) digests (20 μg) were fractionated on a Vydac C18 (4.6 mm inner diameter × 250 mm) column under identical chromatographic conditions. The column was developed at a flow rate of 1 ml min −1 with a linear 120 min gradient from 0 to 70% buffer B, where buffer A was 0.05% (v/v) trifluoroacetic acid in Milli Q water, and buffer B was 0.05% (v/v) trifluoroacetic acid in acetonitrile. Asterisk-labelled peaks within the non-reduced chromatogram represent those altered by DTT reduction. B. Preparative scale RP-HPLC purification of peptides from the tryptic digest of Pf332 DBL domain. A total of 125 μg of digest was fractionated by elution from a Vydac C18 column (4.6 mm inner diameter × 250 mm) column using the elution conditions described in (A). Peptide fractions found to contain disulphide-bonded peptides by Edman degradation and mass spectrometry are indicated by peak numbers and correspond to those given in Table 1 (see Experimental procedures for further details).

    Journal: Molecular Microbiology

    Article Title: Analysis of structure and function of the giant protein Pf332 in Plasmodium falciparum

    doi: 10.1111/j.1365-2958.2008.06508.x

    Figure Lengend Snippet: Identification and purification of disulphide-bonded peptides from a tryptic digest of Pf332 DBL domain. A. Comparison of the analytical RP-HPLC elution profiles for DTT-reduced and non-reduced tryptic digest of refolded Pf332 DBL domain. Reduced (upper trace) and non-reduced (lower trace) digests (20 μg) were fractionated on a Vydac C18 (4.6 mm inner diameter × 250 mm) column under identical chromatographic conditions. The column was developed at a flow rate of 1 ml min −1 with a linear 120 min gradient from 0 to 70% buffer B, where buffer A was 0.05% (v/v) trifluoroacetic acid in Milli Q water, and buffer B was 0.05% (v/v) trifluoroacetic acid in acetonitrile. Asterisk-labelled peaks within the non-reduced chromatogram represent those altered by DTT reduction. B. Preparative scale RP-HPLC purification of peptides from the tryptic digest of Pf332 DBL domain. A total of 125 μg of digest was fractionated by elution from a Vydac C18 column (4.6 mm inner diameter × 250 mm) column using the elution conditions described in (A). Peptide fractions found to contain disulphide-bonded peptides by Edman degradation and mass spectrometry are indicated by peak numbers and correspond to those given in Table 1 (see Experimental procedures for further details).

    Article Snippet: The subdigest of tryptic fraction #35 was analysed after clean-up on C18 ZipTip (Millipore, Bedford, MA, USA) by RP-HPLC on a C18 column (3 μm, 0.15 mm inner diameter × 150 mm, Vydac) using a Surveyor-MS HPLC system (Thermo, San Jose, CA, USA).

    Techniques: Purification, High Performance Liquid Chromatography, Flow Cytometry, Mass Spectrometry

    RP-HPLC Analysis of Propolis from T. Sirindhornae (column: 250 mm X 4.6 mm, 5 μM,λ 250 nm). Standard compounds: caffeic acid (1), apigenin (2), p-coumaric acid (3), and pinocembrin (4) (A). Component analysis of DMEP-A (B), DMEP-B (C) and DMEP-C (D) are indicated by the black lines.

    Journal: Asian Pacific Journal of Cancer Prevention : APJCP

    Article Title: Cytotoxic Activity of Propolis Extracts from the Stingless Bee Trigona Sirindhornae Against Primary and Metastatic Head and Neck Cancer Cell Lines

    doi: 10.22034/APJCP.2017.18.4.1051

    Figure Lengend Snippet: RP-HPLC Analysis of Propolis from T. Sirindhornae (column: 250 mm X 4.6 mm, 5 μM,λ 250 nm). Standard compounds: caffeic acid (1), apigenin (2), p-coumaric acid (3), and pinocembrin (4) (A). Component analysis of DMEP-A (B), DMEP-B (C) and DMEP-C (D) are indicated by the black lines.

    Article Snippet: High performance liquid chromatography (HPLC) analysis Reverse-phase HPLC (RP-HPLC) analysis of the DMEP fractions was performed using an HPLC Spectra system equipped with a P4000 pump and a quaternary gradient pump system, a UV6000LP diode-array detector, and an AS3000 autosampler (Thermo Separation Products, Fremont, CA).

    Techniques: High Performance Liquid Chromatography