reverse phase hplc  (Shimadzu Corporation)

 
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    Name:
    iPad Control Enhances Productivity in HPLC System Operations
    Description:
    iPad Control
    Catalog Number:
    IPAD-CONTROL-ENHANCES-PRODUCTIVITY-IN-HPLC-SYSTEM-OPERATIONS
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    None
    Category:
    Liquid Chromatography iPad Control
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    Structured Review

    Shimadzu Corporation reverse phase hplc
    iPad Control Enhances Productivity in HPLC System Operations
    iPad Control
    https://www.bioz.com/result/reverse phase hplc/product/Shimadzu Corporation
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    reverse phase hplc - by Bioz Stars, 2021-05
    86/100 stars

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    1) Product Images from "Black ginger extract increases physical fitness performance and muscular endurance by improving inflammation and energy metabolism"

    Article Title: Black ginger extract increases physical fitness performance and muscular endurance by improving inflammation and energy metabolism

    Journal: Heliyon

    doi: 10.1016/j.heliyon.2016.e00115

    HPLC chromatogram of KPE for determination of PMFs. PMFs in KPE were separated on a Develosil RPAQUEOUS-AR-5 column. The chromatographic mobile phase consisted of A (acetonitrile: water: acetic acid = 35: 62.5: 2.5, v/v) and B (acetonitrile: acetic acid = 97.5: 2.5, v/v). The gradient program was set as 0–20 min (solvent A: 99–1%). Use of 263 nm as a selective wavelength allowed identification of the 8 known PMFs. Peak 1 , 5-hydroxy-3,7-dimethoxyflavone; 2 , 5-hydroxy-7-methoxyflavone; 3 , 5-hydroxy-3,7,4'-trimethoxyflavone; 4 , 5-hydroxy-3,7,3',4'-tetramethoxyflavone; 5 , 3,5,7,3',4'-pentamethoxyflavone; 6 , 5,7,4'-trimethoxyflavone; 7 , 3,5,7,4'-tetramethoxyflavone; 8 , 5,7-dimethoxyflavone.
    Figure Legend Snippet: HPLC chromatogram of KPE for determination of PMFs. PMFs in KPE were separated on a Develosil RPAQUEOUS-AR-5 column. The chromatographic mobile phase consisted of A (acetonitrile: water: acetic acid = 35: 62.5: 2.5, v/v) and B (acetonitrile: acetic acid = 97.5: 2.5, v/v). The gradient program was set as 0–20 min (solvent A: 99–1%). Use of 263 nm as a selective wavelength allowed identification of the 8 known PMFs. Peak 1 , 5-hydroxy-3,7-dimethoxyflavone; 2 , 5-hydroxy-7-methoxyflavone; 3 , 5-hydroxy-3,7,4'-trimethoxyflavone; 4 , 5-hydroxy-3,7,3',4'-tetramethoxyflavone; 5 , 3,5,7,3',4'-pentamethoxyflavone; 6 , 5,7,4'-trimethoxyflavone; 7 , 3,5,7,4'-tetramethoxyflavone; 8 , 5,7-dimethoxyflavone.

    Techniques Used: High Performance Liquid Chromatography

    Related Articles

    Chromatography:

    Article Title: Bacterial Acyl-CoA Mutase Specifically Catalyzes Coenzyme B12-dependent Isomerization of 2-Hydroxyisobutyryl-CoA and (S)-3-Hydroxybutyryl-CoA *
    Article Snippet: .. Concentrations of acyl-CoA ester substrates and products were quantified by ion-pair chromatography using an HPLC system (Shimadzu) with a Nucleosil 100–5 C18 column (250 mm × 3 mm, 5 μm; Macherey-Nagel) and a mobile phase of 14.5 vol% acetonitrile, 10 m m tetrabutylammonium hydrogen sulfate and 100 m m sodium phosphate at pH 4.5 for the separation of 2-hydroxyisobutyryl-, 3-hydroxybutyryl-, methylmalonyl-, and succinyl-CoA, with retention times of about 35, 24, 30, and 40 min, respectively. ..

    High Performance Liquid Chromatography:

    Article Title: Bacterial Acyl-CoA Mutase Specifically Catalyzes Coenzyme B12-dependent Isomerization of 2-Hydroxyisobutyryl-CoA and (S)-3-Hydroxybutyryl-CoA *
    Article Snippet: .. Concentrations of acyl-CoA ester substrates and products were quantified by ion-pair chromatography using an HPLC system (Shimadzu) with a Nucleosil 100–5 C18 column (250 mm × 3 mm, 5 μm; Macherey-Nagel) and a mobile phase of 14.5 vol% acetonitrile, 10 m m tetrabutylammonium hydrogen sulfate and 100 m m sodium phosphate at pH 4.5 for the separation of 2-hydroxyisobutyryl-, 3-hydroxybutyryl-, methylmalonyl-, and succinyl-CoA, with retention times of about 35, 24, 30, and 40 min, respectively. ..

    Article Title: Membrane-Associated Glucose-Methanol-Choline Oxidoreductase Family Enzymes PhcC and PhcD Are Essential for Enantioselective Catabolism of Dehydrodiconiferyl Alcohol
    Article Snippet: .. To determine the optical rotation of the racemic DCA-C preparation, the sample was separated by an HPLC system (Shimadzu) using a Chiralcel OD-RH column. ..

    Article Title: Controlled-release and preserved bioactivity of proteins from (self-assembled) core-shell double-walled microspheres
    Article Snippet: The mixed solution was incubated at 37°C for 5 minutes, then immediately cooled to 0°C. β-galactosidase activity was determined from absorbance readings for the reaction product of ONPG at 420 nm. .. Size-exclusion chromatography (SEC) SEC assay was carried out using an HPLC system ( Shimadzu, Kyoto, Japan) equipped with a TSK G2000SWXL size-exclusion column. ..

    Article Title: Far Infrared Irradiation Enhances Nutraceutical Compounds and Antioxidant Properties in Angelica gigas Nakai Powder
    Article Snippet: .. HPLC Analysis of AGN Extract with or without FIR Treatments An HPLC system (CBM-20A, Shimadzu Co, Ltd., Kyoto, Japan) with two gradient pump systems (LC-20AT, Shimadzu), a C18 column (Kinetex, 100 × 4.6 mm, 2.6 micron, Phenomenex), an auto-sample injector (SIL-20A, Shimadzu), a UV detector (SPD-10A, Shimadzu) and a column oven (35 °C, CTO-20A, Shimadzu) were used to analyze D and DA. ..

    Article Title: Inhibitory and inductive effects of 4- or 5-methyl-2-mercaptobenzimidazole, thyrotoxic and hepatotoxic rubber antioxidants, on several forms of cytochrome P450 in primary cultured rat and human hepatocytes
    Article Snippet: The supernatant of the incubation medium was extracted with ethyl acetate containing 1 μM 11α-hydroxyprogesterone (Sigma-Aldrich), an internal standard, dried and re-dissolved in 50 % methanol. .. The amount of 6β-hydroxytestosterone in the methanol samples was determined with an HPLC system (LC-10A and SPD-M10Avp, Shimadzu Corp., Kyoto, Japan) with an analysis column (Chemcosorb 5-ODS-H, ∅ 6.0 mm × 150 mm (6A), Chemco Scientific Co. Ltd., Osaka, Japan) and a guard column (Chemcosorb 5-OD-UH, ∅ 4.6 mm × 30 mm (W), Chemco Scientific). .. The analysis conditions were: flow rate, 1 mL/min; injection volume, 100 μL; column temperature, room temperature; mobile phase A, 10 % methanol; mobile phase B, 90 % methanol; gradient, 50 % A for 20 min, 20 % A for 5 min, 5% A for 9 min, and 50 % for 9 min; detection, 240 nm.

    Article Title: Study on health hazards through medicines purchased on the Internet: a cross-sectional investigation of the quality of anti-obesity medicines containing crude drugs as active ingredients
    Article Snippet: .. We carried out quantitative determination of benfluorex, lovastatin, rimonabant, sibutramine hydrochloride, and orlistat with the calibration curves constructed for a HPLC system equipped with a photodiode array (PDA) detector (LC-20 AD; Shimadzu, Kyoto, Japan) according to the method for chemical analyses described in our previous study [ ]. .. The analytical column was a Mightysil RP-18GP (4.6 × 150 mm, 5 μm; Kanto Chemical Co., Inc., Tokyo, Japan).

    Article Title: Integrated chemometric fingerprints of antioxidant activities and HPLC–DAD–CL for assessing the quality of the processed roots of Polygonum multiflorum Thunb. (Heshouwu)
    Article Snippet: Reagent solutions for the determination of peroxynitrite scavenging activity were as follows: a 9 × 10−6 M luminol solution (Solution I) was prepared with 0.1 M carbonate buffer solution (pH 9, NaHCO3 : Na2 CO3 = 9:1) and a 4.97 × 10−4 M peroxynitrite solution (Solution II) was prepared with a 0.1 M NaOH solution on an ice bath. .. HPLC–DAD–CL analysis condition The HPLC system used was a Shimadzu LC-2010C HT system consisting of a quaternary pump, an autosampler (Shimadzu Corporation, Japan), a thermostated column compartment (Shimadzu Corporation, Japan), and a DAD (SPD-M20A, Shimadzu Corporation, Japan). .. HPLC separation was achieved with a Venusil MP C18 column (250 × 4.6 mm, 5 μm, Agela Technologies, China) with a flow rate of 1.0 mL/min.

    Article Title: Diphtheria‐toxin based anti‐human CCR4 immunotoxin for targeting human CCR4+ cells in vivo), Diphtheria-toxin based anti-human CCR4 immunotoxin for targeting human CCR4+ cells in vivo
    Article Snippet: All PCR primers that were used are listed in . .. Anti‐human CCR4 immunotoxins were analyzed with Shimadzu HPLC system using Superdex 200 size‐exclusion column, 10/300 GL (GE healthcare, Cat#: 17‐5175‐01). ..

    Size-exclusion Chromatography:

    Article Title: Controlled-release and preserved bioactivity of proteins from (self-assembled) core-shell double-walled microspheres
    Article Snippet: The mixed solution was incubated at 37°C for 5 minutes, then immediately cooled to 0°C. β-galactosidase activity was determined from absorbance readings for the reaction product of ONPG at 420 nm. .. Size-exclusion chromatography (SEC) SEC assay was carried out using an HPLC system ( Shimadzu, Kyoto, Japan) equipped with a TSK G2000SWXL size-exclusion column. ..

    Construct:

    Article Title: Study on health hazards through medicines purchased on the Internet: a cross-sectional investigation of the quality of anti-obesity medicines containing crude drugs as active ingredients
    Article Snippet: .. We carried out quantitative determination of benfluorex, lovastatin, rimonabant, sibutramine hydrochloride, and orlistat with the calibration curves constructed for a HPLC system equipped with a photodiode array (PDA) detector (LC-20 AD; Shimadzu, Kyoto, Japan) according to the method for chemical analyses described in our previous study [ ]. .. The analytical column was a Mightysil RP-18GP (4.6 × 150 mm, 5 μm; Kanto Chemical Co., Inc., Tokyo, Japan).

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    Shimadzu Corporation preparative reverse phase hplc
    Time-course of the incubation of oxidized <t>AMC</t> in human serum. Notes: Upper panels of ( A – F ) show the <t>HPLC</t> analysis assessed at 0, 0.5, 2, 3, 6, and 24 hours of incubation, respectively; lower panels of ( A – F ) are respective mass spectra at the corresponding retention time. Abbreviations: AMC, antimicrobial cyclic peptide; HPLC, high-performance liquid chromatography; TIC, total ion current.
    Preparative Reverse Phase Hplc, supplied by Shimadzu Corporation, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 1 article reviews
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    99
    Shimadzu Corporation rp hplc
    <t>RP-HPLC</t> profile of <t>F100</t> fraction. Detection was done at wavelength 220 nm.
    Rp Hplc, supplied by Shimadzu Corporation, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Shimadzu Corporation reverse phase high performance liquid chromatography
    Major steps for synthesizing peptide nucleic acids (PNAs). PNAs are synthesized on a solid support following <t>solid-phase</t> synthesis (Step 1) protocols. After the completion of PNA synthesis, it is cleaved from the solid support and purified via <t>reverse</t> phase <t>high</t> <t>performance</t> <t>liquid</t> <t>chromatography</t> (RP-HPLC) (Step 2) and the mass of the purified PNA (Step 3) is determined via Matrix Assisted Laser Desorption/ Ionization Time of Flight (MALDI-TOF) spectroscopy.
    Reverse Phase High Performance Liquid Chromatography, supplied by Shimadzu Corporation, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Shimadzu Corporation rp hplc analysis
    Characterization of <t>REPS:</t> ( A ) <t>RP-HPLC</t> chromatogram of REPS solution (10 mg/mL) using C 18 column at 0.8 mL/min flow rate and the following gradient: 0–5 min, 0%; 5–50 min, 60%; 50–55 min, 60%; 55–60 min, 90%; and 60–65 min, 90% of solvent B (80% v / v CH 3 CN and 0.1% v / v TFA). Inset: UV-vis absorption spectrum of REPS solution. ( B ) FT-IR spectrum of REPS showing signals within 4000 to 250 cm −1 ; the measurements were consistent among three replicates.
    Rp Hplc Analysis, supplied by Shimadzu Corporation, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Time-course of the incubation of oxidized AMC in human serum. Notes: Upper panels of ( A – F ) show the HPLC analysis assessed at 0, 0.5, 2, 3, 6, and 24 hours of incubation, respectively; lower panels of ( A – F ) are respective mass spectra at the corresponding retention time. Abbreviations: AMC, antimicrobial cyclic peptide; HPLC, high-performance liquid chromatography; TIC, total ion current.

    Journal: International Journal of Nanomedicine

    Article Title: Design and activity of a cyclic mini-β-defensin analog: a novel antimicrobial tool

    doi: 10.2147/IJN.S89610

    Figure Lengend Snippet: Time-course of the incubation of oxidized AMC in human serum. Notes: Upper panels of ( A – F ) show the HPLC analysis assessed at 0, 0.5, 2, 3, 6, and 24 hours of incubation, respectively; lower panels of ( A – F ) are respective mass spectra at the corresponding retention time. Abbreviations: AMC, antimicrobial cyclic peptide; HPLC, high-performance liquid chromatography; TIC, total ion current.

    Article Snippet: The oxidized AMC analog was purified by preparative reverse-phase HPLC (Shimadzu SPD-10A VP UV-Vis detector) using a gradient of acetonitrile (0.1% TFA) in water from 5% to 70% for 20 minutes and a Jupiter column (10 µm, Proteo 90 Å, 250×21 mm).

    Techniques: Incubation, High Performance Liquid Chromatography

    RP-HPLC profile of F100 fraction. Detection was done at wavelength 220 nm.

    Journal: BioMed Research International

    Article Title: Characterisation of Potential Antidiabetic-Related Proteins from Pleurotus pulmonarius (Fr.) Quél. (Grey Oyster Mushroom) by MALDI-TOF/TOF Mass Spectrometry

    doi: 10.1155/2014/131607

    Figure Lengend Snippet: RP-HPLC profile of F100 fraction. Detection was done at wavelength 220 nm.

    Article Snippet: In view of this data, all fractions of F100 eluted from the RP-HPLC (peak 1–6) were combined and evaluated for α-amylase activity.

    Techniques: High Performance Liquid Chromatography

    Major steps for synthesizing peptide nucleic acids (PNAs). PNAs are synthesized on a solid support following solid-phase synthesis (Step 1) protocols. After the completion of PNA synthesis, it is cleaved from the solid support and purified via reverse phase high performance liquid chromatography (RP-HPLC) (Step 2) and the mass of the purified PNA (Step 3) is determined via Matrix Assisted Laser Desorption/ Ionization Time of Flight (MALDI-TOF) spectroscopy.

    Journal: MethodsX

    Article Title: Formulation of PLGA nanoparticles containing short cationic peptide nucleic acids

    doi: 10.1016/j.mex.2020.101115

    Figure Lengend Snippet: Major steps for synthesizing peptide nucleic acids (PNAs). PNAs are synthesized on a solid support following solid-phase synthesis (Step 1) protocols. After the completion of PNA synthesis, it is cleaved from the solid support and purified via reverse phase high performance liquid chromatography (RP-HPLC) (Step 2) and the mass of the purified PNA (Step 3) is determined via Matrix Assisted Laser Desorption/ Ionization Time of Flight (MALDI-TOF) spectroscopy.

    Article Snippet: Combine the dissolved crude PNAs in 1.5 mL tube and centrifuge the tube at 10,000 rpm for 5 min. Start the purification of crude PNA using Reverse Phase High Performance Liquid Chromatography (RP-HPLC, Shimadzu) with automatic fraction collector and XBridge® BEH C18 OBDTM Prep Column.

    Techniques: Synthesized, Purification, High Performance Liquid Chromatography, Spectroscopy

    Characterization of REPS: ( A ) RP-HPLC chromatogram of REPS solution (10 mg/mL) using C 18 column at 0.8 mL/min flow rate and the following gradient: 0–5 min, 0%; 5–50 min, 60%; 50–55 min, 60%; 55–60 min, 90%; and 60–65 min, 90% of solvent B (80% v / v CH 3 CN and 0.1% v / v TFA). Inset: UV-vis absorption spectrum of REPS solution. ( B ) FT-IR spectrum of REPS showing signals within 4000 to 250 cm −1 ; the measurements were consistent among three replicates.

    Journal: Marine Drugs

    Article Title: Trichormus variabilis (Cyanobacteria) Biomass: From the Nutraceutical Products to Novel EPS-Cell/Protein Carrier Systems

    doi: 10.3390/md16090298

    Figure Lengend Snippet: Characterization of REPS: ( A ) RP-HPLC chromatogram of REPS solution (10 mg/mL) using C 18 column at 0.8 mL/min flow rate and the following gradient: 0–5 min, 0%; 5–50 min, 60%; 50–55 min, 60%; 55–60 min, 90%; and 60–65 min, 90% of solvent B (80% v / v CH 3 CN and 0.1% v / v TFA). Inset: UV-vis absorption spectrum of REPS solution. ( B ) FT-IR spectrum of REPS showing signals within 4000 to 250 cm −1 ; the measurements were consistent among three replicates.

    Article Snippet: The RP-HPLC analysis of the REPS solutions was performed using LC-10AVP equipment (Shimadzu, Milan, Italy), 0.1% v /v trifluoroacetic acid as solvent A and 80% v /v acetonitrile and 0.1% v /v trifluoroacetic acid as solvent B.

    Techniques: High Performance Liquid Chromatography, Flow Cytometry