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Phenomenex reverse phase hplc column
Reverse Phase Hplc Column, supplied by Phenomenex, used in various techniques. Bioz Stars score: 91/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/reverse phase hplc column/product/Phenomenex
Average 91 stars, based on 28 article reviews
Price from $9.99 to $1999.99
reverse phase hplc column - by Bioz Stars, 2020-11
91/100 stars

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High Performance Liquid Chromatography:

Article Title: A fluorescence polarization binding assay to identify inhibitors of flavin-dependent monooxygenases
Article Snippet: .. After stirring at rt for 2 h, the solution was diluted with H2 O (1.9 mL) and acetonitrile (0.1 mL), injected onto a reverse-phase HPLC column (Phenomenex Luna C18 (2) column, 250 × 4.6 mm, 5 micron) and purified at a flow rate of 1.0 mL/min with linear gradient elution (5% to 65% acetonitrile in H2 O over 55 min, then 65% to 95% over 5 min) to afford chromophore 1 (1.2 mg, 69%). ..

Article Title: Direct Modification of the Proinflammatory Cytokine Macrophage Migration Inhibitory Factor by Dietary Isothiocyanates *
Article Snippet: .. One peak detected at 270 nm on a Jasco PU 980 HPLC system with a reverse phase HPLC column (Prodigy 5-μm C18 column, 250 × 4 mm, Phenomenex, San José, CA) eluted with 50% acetonitrile, 50% water containing 0.1% trifluoroacetic acid at a rate of 1 ml/min. .. 1 H NMR (500 MHz, chloroform-d1 ) δ 7.6 (3H, broad, NH3 ), 7.33 (1H, t, J = 7.5 Hz, ArH), 7.14 (2H, broad t, J = 8 Hz, ArH), 7.09 (1H, broad s, ArH), 3.72 (2H, t, J = 6.5 Hz, CH2 NCS), 3.30 (2H,m, CH2 NH3 ), 3.0 (4H, m, CH2 CH2 NCS + CH2 CH2 NH3 ) ppm.

Article Title: Topical electrophilic nitro-fatty acids potentiate cutaneous inflammation
Article Snippet: .. Mouse skin lipid extracts were resolved for quantitation purposes using a reverse phase HPLC column [2 × 20 mm C18 Mercury column (Phenomenex; Torrance, CA)] with a gradient solvent system consisting of solvents (A): H2 O containing 0.1% acetic acid and (B): acetonitrile containing 0.1% acetic acid, at a 0.75 ml/min flow rate. ..

Article Title: Expression and Biological Activity of the Cystine Knot Bioinsecticide PA1b (Pea Albumin 1 Subunit b)
Article Snippet: .. The powder was re-suspended in 60% EtOH (10 mg.mL−1 ) and injected onto a reverse-phase HPLC column (Jupiter C18 5 µm, 300 A, 250×4.6 mm, Phenomenex), in an Agilent 1200 apparatus with elution, as described in . ..

Article Title: Synthesis of [18F]Xenon Difluoride as a Radiolabeling Reagent from [18F]Fluoride Ion in a Micro-reactor and at Production Scale
Article Snippet: .. The reaction mixture immediately turned from blue to brown and was left standing at room temperature for 30–60 min. An aliquot of the reaction mixture (10 μL) was quenched with acetonitrile (300 μL) in a plastic vial (PP LV, 500 μL, Grace) and a sample (20 μL) was then analyzed on a reverse phase HPLC column (Phenomenex, C18, 5 μ, 250 × 4.6 mm) eluted at 1 mL/min with aq. ..

Article Title: Characterization and quantification of endogenous fatty acid nitroalkene metabolites in human urine [S]
Article Snippet: .. Lipid extracts were resolved for quantitation using a reverse phase HPLC column (2 × 20 mm C18 mercury column; Phenomenex) at a 0.75 ml/min flow rate. ..

Article Title: Source and role of intestinally derived lysophosphatidic acid in dyslipidemia and atherosclerosis
Article Snippet: .. The extracted samples were dried under a nitrogen stream (30°C–37°C) and dissolved in methanol (75 μl), eluent A (25 μl, 10 mM ammonium acetate, pH 8.5) was added, samples were vortexed, and an aliquot (20 μl) was injected onto a reverse phase HPLC column (Luna C5, 5 μm, 150 × 2 mm with a guard column; Phenomenex, Torrance, CA) equilibrated in 65% eluent A and 35% eluent B (acetonitrile-methanol, 98:2, v/v) and eluted (200 μl/min) with an increasing concentration of eluent B (min/%B; 0/35, 0.5/35, 11.5/80, 12/35, 16/35). .. The effluent from the column was directed to an electrospray ion source (Agilent Jet Stream) connected to a triple quadrupole mass spectrometer (Agilent 6460, Santa Clara, CA) operating in the negative ion multiple reaction monitoring mode in which the intensities of specific parent→fragment ion transitions were recorded [LPA 20:4 (LPA with arachidonic acid, at sn -1 and a hydroxyl group at sn -2), 457→153, retention time 7.6 min; LPA 17:0, 423→153, internal standard, retention time 8.6 min; LPA 16:0, 409→153, retention time 7.8 min; LPA 18:0, 437→153, retention time 9.4 min; LPA 18:1, 435→153, retention time 8.3 min; LPA 18:2, 433→153, retention time 7.5 min] under previously optimized conditions (fragmentor voltage, collision energy, and cell accelerator voltages of 140, 14, and 7, respectively; Q1 and Q3 used with FWHM settings of Widest and Unit, respectively).

Flow Cytometry:

Article Title: A fluorescence polarization binding assay to identify inhibitors of flavin-dependent monooxygenases
Article Snippet: .. After stirring at rt for 2 h, the solution was diluted with H2 O (1.9 mL) and acetonitrile (0.1 mL), injected onto a reverse-phase HPLC column (Phenomenex Luna C18 (2) column, 250 × 4.6 mm, 5 micron) and purified at a flow rate of 1.0 mL/min with linear gradient elution (5% to 65% acetonitrile in H2 O over 55 min, then 65% to 95% over 5 min) to afford chromophore 1 (1.2 mg, 69%). ..

Article Title: Topical electrophilic nitro-fatty acids potentiate cutaneous inflammation
Article Snippet: .. Mouse skin lipid extracts were resolved for quantitation purposes using a reverse phase HPLC column [2 × 20 mm C18 Mercury column (Phenomenex; Torrance, CA)] with a gradient solvent system consisting of solvents (A): H2 O containing 0.1% acetic acid and (B): acetonitrile containing 0.1% acetic acid, at a 0.75 ml/min flow rate. ..

Article Title: Characterization and quantification of endogenous fatty acid nitroalkene metabolites in human urine [S]
Article Snippet: .. Lipid extracts were resolved for quantitation using a reverse phase HPLC column (2 × 20 mm C18 mercury column; Phenomenex) at a 0.75 ml/min flow rate. ..

Multiple Displacement Amplification:

Article Title: Diabetes and increased lipid peroxidation are associated with systemic inflammation even in well-controlled patients
Article Snippet: .. The assessment of MDA in plasma was determined by HPLC (Shimadzu, Tokyo, Japan) with a reverse-phase HPLC column (C18; 4.6 × 150 mm) (Phenomenex, Torrance, CA, USA) and compared with MDA standard curves. .. The levels of total antioxidant capacity of plasma were evaluated with a commercially available colorimetric kit (Cayman Chemical Company®, Ann Arbor, MI, USA) following the manufacturer's instructions.

Purification:

Article Title: A fluorescence polarization binding assay to identify inhibitors of flavin-dependent monooxygenases
Article Snippet: .. After stirring at rt for 2 h, the solution was diluted with H2 O (1.9 mL) and acetonitrile (0.1 mL), injected onto a reverse-phase HPLC column (Phenomenex Luna C18 (2) column, 250 × 4.6 mm, 5 micron) and purified at a flow rate of 1.0 mL/min with linear gradient elution (5% to 65% acetonitrile in H2 O over 55 min, then 65% to 95% over 5 min) to afford chromophore 1 (1.2 mg, 69%). ..

Concentration Assay:

Article Title: Source and role of intestinally derived lysophosphatidic acid in dyslipidemia and atherosclerosis
Article Snippet: .. The extracted samples were dried under a nitrogen stream (30°C–37°C) and dissolved in methanol (75 μl), eluent A (25 μl, 10 mM ammonium acetate, pH 8.5) was added, samples were vortexed, and an aliquot (20 μl) was injected onto a reverse phase HPLC column (Luna C5, 5 μm, 150 × 2 mm with a guard column; Phenomenex, Torrance, CA) equilibrated in 65% eluent A and 35% eluent B (acetonitrile-methanol, 98:2, v/v) and eluted (200 μl/min) with an increasing concentration of eluent B (min/%B; 0/35, 0.5/35, 11.5/80, 12/35, 16/35). .. The effluent from the column was directed to an electrospray ion source (Agilent Jet Stream) connected to a triple quadrupole mass spectrometer (Agilent 6460, Santa Clara, CA) operating in the negative ion multiple reaction monitoring mode in which the intensities of specific parent→fragment ion transitions were recorded [LPA 20:4 (LPA with arachidonic acid, at sn -1 and a hydroxyl group at sn -2), 457→153, retention time 7.6 min; LPA 17:0, 423→153, internal standard, retention time 8.6 min; LPA 16:0, 409→153, retention time 7.8 min; LPA 18:0, 437→153, retention time 9.4 min; LPA 18:1, 435→153, retention time 8.3 min; LPA 18:2, 433→153, retention time 7.5 min] under previously optimized conditions (fragmentor voltage, collision energy, and cell accelerator voltages of 140, 14, and 7, respectively; Q1 and Q3 used with FWHM settings of Widest and Unit, respectively).

Quantitation Assay:

Article Title: Topical electrophilic nitro-fatty acids potentiate cutaneous inflammation
Article Snippet: .. Mouse skin lipid extracts were resolved for quantitation purposes using a reverse phase HPLC column [2 × 20 mm C18 Mercury column (Phenomenex; Torrance, CA)] with a gradient solvent system consisting of solvents (A): H2 O containing 0.1% acetic acid and (B): acetonitrile containing 0.1% acetic acid, at a 0.75 ml/min flow rate. ..

Article Title: Characterization and quantification of endogenous fatty acid nitroalkene metabolites in human urine [S]
Article Snippet: .. Lipid extracts were resolved for quantitation using a reverse phase HPLC column (2 × 20 mm C18 mercury column; Phenomenex) at a 0.75 ml/min flow rate. ..

Injection:

Article Title: A fluorescence polarization binding assay to identify inhibitors of flavin-dependent monooxygenases
Article Snippet: .. After stirring at rt for 2 h, the solution was diluted with H2 O (1.9 mL) and acetonitrile (0.1 mL), injected onto a reverse-phase HPLC column (Phenomenex Luna C18 (2) column, 250 × 4.6 mm, 5 micron) and purified at a flow rate of 1.0 mL/min with linear gradient elution (5% to 65% acetonitrile in H2 O over 55 min, then 65% to 95% over 5 min) to afford chromophore 1 (1.2 mg, 69%). ..

Article Title: Expression and Biological Activity of the Cystine Knot Bioinsecticide PA1b (Pea Albumin 1 Subunit b)
Article Snippet: .. The powder was re-suspended in 60% EtOH (10 mg.mL−1 ) and injected onto a reverse-phase HPLC column (Jupiter C18 5 µm, 300 A, 250×4.6 mm, Phenomenex), in an Agilent 1200 apparatus with elution, as described in . ..

Article Title: Source and role of intestinally derived lysophosphatidic acid in dyslipidemia and atherosclerosis
Article Snippet: .. The extracted samples were dried under a nitrogen stream (30°C–37°C) and dissolved in methanol (75 μl), eluent A (25 μl, 10 mM ammonium acetate, pH 8.5) was added, samples were vortexed, and an aliquot (20 μl) was injected onto a reverse phase HPLC column (Luna C5, 5 μm, 150 × 2 mm with a guard column; Phenomenex, Torrance, CA) equilibrated in 65% eluent A and 35% eluent B (acetonitrile-methanol, 98:2, v/v) and eluted (200 μl/min) with an increasing concentration of eluent B (min/%B; 0/35, 0.5/35, 11.5/80, 12/35, 16/35). .. The effluent from the column was directed to an electrospray ion source (Agilent Jet Stream) connected to a triple quadrupole mass spectrometer (Agilent 6460, Santa Clara, CA) operating in the negative ion multiple reaction monitoring mode in which the intensities of specific parent→fragment ion transitions were recorded [LPA 20:4 (LPA with arachidonic acid, at sn -1 and a hydroxyl group at sn -2), 457→153, retention time 7.6 min; LPA 17:0, 423→153, internal standard, retention time 8.6 min; LPA 16:0, 409→153, retention time 7.8 min; LPA 18:0, 437→153, retention time 9.4 min; LPA 18:1, 435→153, retention time 8.3 min; LPA 18:2, 433→153, retention time 7.5 min] under previously optimized conditions (fragmentor voltage, collision energy, and cell accelerator voltages of 140, 14, and 7, respectively; Q1 and Q3 used with FWHM settings of Widest and Unit, respectively).

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  • 88
    Phenomenex reverse phase c18 hplc column
    Demonstration of oxidized PS species in apoptotic membranes using negative ion LC/MS/MS. The presence of oxidized PAPS or PLPS species in lipid extracts of apoptotic cells was analyzed by <t>HPLC</t> with on-line negative ion electrospray ionization tandem mass spectrometry. Separation was done on a reverse phase <t>C18</t> column (2.1 × 250 mm, 5μm) using methanol/water as mobile phase at a flow rate of 0.2 ml/min with gradient and oxidized PLPS (a) and PAPS (b) molecular species identified by their characteristic retention time and daughter ions specific for each analyte, as described in Materials and methods.
    Reverse Phase C18 Hplc Column, supplied by Phenomenex, used in various techniques. Bioz Stars score: 88/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/reverse phase c18 hplc column/product/Phenomenex
    Average 88 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    reverse phase c18 hplc column - by Bioz Stars, 2020-11
    88/100 stars
      Buy from Supplier

    85
    Phenomenex reverse phase high performance liquid chromatography rp hplc column
    Isolation and identification of the neurokinin Thr 6 -BK extracted from the venom of the social wasp, P. occidentalis. ( a ) <t>RP-HPLC</t> profile of the low MW extract from the venom of P. occidentalis. Chromatography was carried out with a C 18 <t>ODS</t> column at a flow rate of 5.0 ml min −1 and eluted by a linear gradient from 2% ACN/H 2 O (v/v) (containing 0.07% TFA) for 20 min, followed by 2–60% for 40 min and 60% for 20 min. ( b ) Chromatographic profile of purification of the fraction indicated by arrow in A. RP-HPLC using a C 18 column at a flow rate of 1.0 ml min −1 and eluted by a linear gradient from 2% ACN/H 2 O (v/v) (containing 0.07% TFA) for 10 min, followed by 2–60% for 20 min and 60% for 10 min. ( c ) ESI mass spectrum of the Thr 6 -BK, presenting a major peak at m/z 1074.8.
    Reverse Phase High Performance Liquid Chromatography Rp Hplc Column, supplied by Phenomenex, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/reverse phase high performance liquid chromatography rp hplc column/product/Phenomenex
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    reverse phase high performance liquid chromatography rp hplc column - by Bioz Stars, 2020-11
    85/100 stars
      Buy from Supplier

    91
    Phenomenex c18 rp hplc column
    Distinct HRNR-derived CIDAMPs show similar ribosomal protein binding patterns. ( a ) SulfoLink®–column-bound proteins of an E . coli -extract were separated on a Jupiter® <t>C18</t> <t>RP-HPLC</t> column with a Prp-gradient. HPLC fractions containing UV-absorbing peaks (C1–D10) were divided into five aliquots and adjusted in parallel to five PAGE-gels and separated. ( b ) Silver-stained proteins. ( c ) HRNR-Far-Western blot for probing with biotinylated HR1-18 (HRNR 2556–2677 ) using Strep - Tactin ®, ( d ) HRNR-Far-Western blot for probing with biotinylated rSumo3-HRNR 2591–2684 using Strep - Tactin ®, ( e ) HRNR-Far-Western blot for probing with rHRNR 2591–2684 using anti-HRNR 2591–2684 antibodies, ( f ) HRNR-Far-Western blot for probing with rHRNR 1075–1172 using anti-HRNR 1075–1172 antibodies. Note similarities of the staining patterns, irrespective the CIDAMP AA-sequence or biotin-labeling and irrespective whether a Strep - Tactin ®- or antibody-detectable CIDAMP was used to probe and detect the target protein on the membrane. Note the presence of 70 kDa bands upon HRNR-Far-Western blot analyses in most of the investigated HPLC fractions with highest intensity for rHRNR 1075–1172 binding ( f ). The most intensive band, corresponding to a 37 kDa protein in fraction number C13, was identified as E . coli ribosomal protein L2.
    C18 Rp Hplc Column, supplied by Phenomenex, used in various techniques. Bioz Stars score: 91/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/c18 rp hplc column/product/Phenomenex
    Average 91 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    c18 rp hplc column - by Bioz Stars, 2020-11
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    88
    Phenomenex rp hplc columns
    Comparative chromatograms showing isocratic <t>RP-HPLC</t> separation of mixed standard, L-citrulline, and L-arginine from 2 different columns: (a) <t>Zorbax</t> Eclipse XDB-C 18 , 5 μ m, and (b) Gemini C 18 , 3 μ m; efficient separation and the best resolution were achieved by the Gemini C 18 column which showed that compounds are well separated. The peaks marked represent (1) L-arginine and (2) L-citrulline.
    Rp Hplc Columns, supplied by Phenomenex, used in various techniques. Bioz Stars score: 88/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 88 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    rp hplc columns - by Bioz Stars, 2020-11
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    Demonstration of oxidized PS species in apoptotic membranes using negative ion LC/MS/MS. The presence of oxidized PAPS or PLPS species in lipid extracts of apoptotic cells was analyzed by HPLC with on-line negative ion electrospray ionization tandem mass spectrometry. Separation was done on a reverse phase C18 column (2.1 × 250 mm, 5μm) using methanol/water as mobile phase at a flow rate of 0.2 ml/min with gradient and oxidized PLPS (a) and PAPS (b) molecular species identified by their characteristic retention time and daughter ions specific for each analyte, as described in Materials and methods.

    Journal: The Journal of Experimental Medicine

    Article Title: Oxidized phosphatidylserine-CD36 interactions play an essential role in macrophage-dependent phagocytosis of apoptotic cells

    doi: 10.1084/jem.20060370

    Figure Lengend Snippet: Demonstration of oxidized PS species in apoptotic membranes using negative ion LC/MS/MS. The presence of oxidized PAPS or PLPS species in lipid extracts of apoptotic cells was analyzed by HPLC with on-line negative ion electrospray ionization tandem mass spectrometry. Separation was done on a reverse phase C18 column (2.1 × 250 mm, 5μm) using methanol/water as mobile phase at a flow rate of 0.2 ml/min with gradient and oxidized PLPS (a) and PAPS (b) molecular species identified by their characteristic retention time and daughter ions specific for each analyte, as described in Materials and methods.

    Article Snippet: Each sample preparation (in 90% methanol) was injected onto a reverse phase C18 HPLC column (2 × 150 mm, 5 μm; ODS; Phenomenex) at a flow rate of 0.2 ml/min. oxPSes were resolved using a gradient from water containing 0.2% ammonium to methanol containing 0.2% ammonium.

    Techniques: Liquid Chromatography with Mass Spectroscopy, Mass Spectrometry, Papanicolaou Stain, High Performance Liquid Chromatography, Flow Cytometry

    Isolation and identification of the neurokinin Thr 6 -BK extracted from the venom of the social wasp, P. occidentalis. ( a ) RP-HPLC profile of the low MW extract from the venom of P. occidentalis. Chromatography was carried out with a C 18 ODS column at a flow rate of 5.0 ml min −1 and eluted by a linear gradient from 2% ACN/H 2 O (v/v) (containing 0.07% TFA) for 20 min, followed by 2–60% for 40 min and 60% for 20 min. ( b ) Chromatographic profile of purification of the fraction indicated by arrow in A. RP-HPLC using a C 18 column at a flow rate of 1.0 ml min −1 and eluted by a linear gradient from 2% ACN/H 2 O (v/v) (containing 0.07% TFA) for 10 min, followed by 2–60% for 20 min and 60% for 10 min. ( c ) ESI mass spectrum of the Thr 6 -BK, presenting a major peak at m/z 1074.8.

    Journal: British Journal of Pharmacology

    Article Title: Inhibition of acute nociceptive responses in rats after i.c.v. injection of Thr6-bradykinin, isolated from the venom of the social wasp, Polybia occidentalis

    doi: 10.1038/sj.bjp.0707275

    Figure Lengend Snippet: Isolation and identification of the neurokinin Thr 6 -BK extracted from the venom of the social wasp, P. occidentalis. ( a ) RP-HPLC profile of the low MW extract from the venom of P. occidentalis. Chromatography was carried out with a C 18 ODS column at a flow rate of 5.0 ml min −1 and eluted by a linear gradient from 2% ACN/H 2 O (v/v) (containing 0.07% TFA) for 20 min, followed by 2–60% for 40 min and 60% for 20 min. ( b ) Chromatographic profile of purification of the fraction indicated by arrow in A. RP-HPLC using a C 18 column at a flow rate of 1.0 ml min −1 and eluted by a linear gradient from 2% ACN/H 2 O (v/v) (containing 0.07% TFA) for 10 min, followed by 2–60% for 20 min and 60% for 10 min. ( c ) ESI mass spectrum of the Thr 6 -BK, presenting a major peak at m/z 1074.8.

    Article Snippet: The solution was chromatographed on a reverse-phase high-performance liquid chromatography (RP-HPLC) column (C18 ODS, Jupiter 15 μ m, 20 × 250 mm, Phenomenex, Torrence, CA, USA) at a flow rate of 5.0 ml/min and eluted using an isocratic gradient from 2% ACN/H2 O (v/v) (containing 0.07% TFA) for 20 min, followed by 2–60% for 40 min and 60% for 20 min.

    Techniques: Isolation, High Performance Liquid Chromatography, Chromatography, Flow Cytometry, Purification

    Distinct HRNR-derived CIDAMPs show similar ribosomal protein binding patterns. ( a ) SulfoLink®–column-bound proteins of an E . coli -extract were separated on a Jupiter® C18 RP-HPLC column with a Prp-gradient. HPLC fractions containing UV-absorbing peaks (C1–D10) were divided into five aliquots and adjusted in parallel to five PAGE-gels and separated. ( b ) Silver-stained proteins. ( c ) HRNR-Far-Western blot for probing with biotinylated HR1-18 (HRNR 2556–2677 ) using Strep - Tactin ®, ( d ) HRNR-Far-Western blot for probing with biotinylated rSumo3-HRNR 2591–2684 using Strep - Tactin ®, ( e ) HRNR-Far-Western blot for probing with rHRNR 2591–2684 using anti-HRNR 2591–2684 antibodies, ( f ) HRNR-Far-Western blot for probing with rHRNR 1075–1172 using anti-HRNR 1075–1172 antibodies. Note similarities of the staining patterns, irrespective the CIDAMP AA-sequence or biotin-labeling and irrespective whether a Strep - Tactin ®- or antibody-detectable CIDAMP was used to probe and detect the target protein on the membrane. Note the presence of 70 kDa bands upon HRNR-Far-Western blot analyses in most of the investigated HPLC fractions with highest intensity for rHRNR 1075–1172 binding ( f ). The most intensive band, corresponding to a 37 kDa protein in fraction number C13, was identified as E . coli ribosomal protein L2.

    Journal: Scientific Reports

    Article Title: Hornerin contains a Linked Series of Ribosome-Targeting Peptide Antibiotics

    doi: 10.1038/s41598-018-34467-8

    Figure Lengend Snippet: Distinct HRNR-derived CIDAMPs show similar ribosomal protein binding patterns. ( a ) SulfoLink®–column-bound proteins of an E . coli -extract were separated on a Jupiter® C18 RP-HPLC column with a Prp-gradient. HPLC fractions containing UV-absorbing peaks (C1–D10) were divided into five aliquots and adjusted in parallel to five PAGE-gels and separated. ( b ) Silver-stained proteins. ( c ) HRNR-Far-Western blot for probing with biotinylated HR1-18 (HRNR 2556–2677 ) using Strep - Tactin ®, ( d ) HRNR-Far-Western blot for probing with biotinylated rSumo3-HRNR 2591–2684 using Strep - Tactin ®, ( e ) HRNR-Far-Western blot for probing with rHRNR 2591–2684 using anti-HRNR 2591–2684 antibodies, ( f ) HRNR-Far-Western blot for probing with rHRNR 1075–1172 using anti-HRNR 1075–1172 antibodies. Note similarities of the staining patterns, irrespective the CIDAMP AA-sequence or biotin-labeling and irrespective whether a Strep - Tactin ®- or antibody-detectable CIDAMP was used to probe and detect the target protein on the membrane. Note the presence of 70 kDa bands upon HRNR-Far-Western blot analyses in most of the investigated HPLC fractions with highest intensity for rHRNR 1075–1172 binding ( f ). The most intensive band, corresponding to a 37 kDa protein in fraction number C13, was identified as E . coli ribosomal protein L2.

    Article Snippet: We either used a Jupiter® 300 Å, 250 × 12.6 mm, C18-RP-HPLC column (Phenomenex) or an Aeris® widepore XB C18, 250 × 12.6 mm, RP-HPLC column (Phenomenex) and either a gradient of acetonitrile (ACN) in aqueous 0.1% TFA, or a gradient of 2-propanol (Prp) in aqueous 0.1% TFA – as indicated for separation of ribosomal proteins.

    Techniques: Derivative Assay, Protein Binding, High Performance Liquid Chromatography, Polyacrylamide Gel Electrophoresis, Staining, Far Western Blot, Sequencing, Labeling, Binding Assay

    Comparative chromatograms showing isocratic RP-HPLC separation of mixed standard, L-citrulline, and L-arginine from 2 different columns: (a) Zorbax Eclipse XDB-C 18 , 5 μ m, and (b) Gemini C 18 , 3 μ m; efficient separation and the best resolution were achieved by the Gemini C 18 column which showed that compounds are well separated. The peaks marked represent (1) L-arginine and (2) L-citrulline.

    Journal: International Journal of Analytical Chemistry

    Article Title: Development of Isocratic RP-HPLC Method for Separation and Quantification of L-Citrulline and L-Arginine in Watermelons

    doi: 10.1155/2018/4798530

    Figure Lengend Snippet: Comparative chromatograms showing isocratic RP-HPLC separation of mixed standard, L-citrulline, and L-arginine from 2 different columns: (a) Zorbax Eclipse XDB-C 18 , 5 μ m, and (b) Gemini C 18 , 3 μ m; efficient separation and the best resolution were achieved by the Gemini C 18 column which showed that compounds are well separated. The peaks marked represent (1) L-arginine and (2) L-citrulline.

    Article Snippet: The RP-HPLC columns [i.e., Zorbax Eclipse XDB-C18 , 250 mm × 4.6 mm, 80 Å, 5 μ m (Phenomenex, Torrance, CA), and Gemini C18 , 250 × 4.6 mm, 110 Å, 3 μ m (Phenomenex, Torrance, CA)] were used.

    Techniques: High Performance Liquid Chromatography