restriction enzymes ncoi  (New England Biolabs)


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    Structured Review

    New England Biolabs restriction enzymes ncoi
    The Double Digestion Patterns of the pNZ8148-HPV16-optiE7 Shuttle Plasmid via <t>NcoI</t> and <t>SacI</t> Restriction Endonuclease
    Restriction Enzymes Ncoi, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/restriction enzymes ncoi/product/New England Biolabs
    Average 96 stars, based on 40 article reviews
    Price from $9.99 to $1999.99
    restriction enzymes ncoi - by Bioz Stars, 2022-11
    96/100 stars

    Images

    1) Product Images from "Codon Usage Optimization and Construction of Plasmid Encoding Iranian Human Papillomavirus Type 16 E7 Oncogene for Lactococcus Lactis Subsp. Cremoris MG1363"

    Article Title: Codon Usage Optimization and Construction of Plasmid Encoding Iranian Human Papillomavirus Type 16 E7 Oncogene for Lactococcus Lactis Subsp. Cremoris MG1363

    Journal: Asian Pacific Journal of Cancer Prevention : APJCP

    doi: 10.22034/APJCP.2017.18.3.783

    The Double Digestion Patterns of the pNZ8148-HPV16-optiE7 Shuttle Plasmid via NcoI and SacI Restriction Endonuclease
    Figure Legend Snippet: The Double Digestion Patterns of the pNZ8148-HPV16-optiE7 Shuttle Plasmid via NcoI and SacI Restriction Endonuclease

    Techniques Used: Plasmid Preparation

    2) Product Images from "Protein switches identified from diverse insertion libraries created using S1 nuclease digestion of supercoiled-form plasmid DNA"

    Article Title: Protein switches identified from diverse insertion libraries created using S1 nuclease digestion of supercoiled-form plasmid DNA

    Journal: Biotechnology and bioengineering

    doi: 10.1002/bit.23224

    S1 nuclease converts supercoiled plasmid DNA to nicked circular and linear forms through digestion at a variety of locations in the plasmid. (A) Two μg of supercoiled pRH04.152-rbsB was incubated at 22°C (top) or 37°C (bottom) with different amounts of S1 nuclease for different lengths of time and then analyzed by agarose gel electrophoresis. The sixth and seventh lanes contain undigested supercoiled control and SpeI-digested linear control, respectively. The band that runs slower than the linear band was presumed to correspond to nicked circular plasmid. In the thirteenth lane, after the DNA was digested with 10 units for 24 hours an additional 10 units of S1 nuclease was added and the DNA was incubated for an additional hour. The first and last lanes contain the λDNA/HindIII molecular weight standards. (B) Plasmids with or without the f1 origin (pDIMC8-pfMBP and pRH04-pfMBP, respectively) were incubated with or without S1 nuclease, followed by incubation with or without NcoI, EagI, and SacII and then analyzed by agarose gel electrophoresis. (C) Plasmids with or without the f1 origin (pDIMC8-rbsb and pRH04-rbsb, respectively) were incubated with S1 nuclease or DNaseI, followed by incubation with XmnI and analyzed by agarose gel electrophoresis.
    Figure Legend Snippet: S1 nuclease converts supercoiled plasmid DNA to nicked circular and linear forms through digestion at a variety of locations in the plasmid. (A) Two μg of supercoiled pRH04.152-rbsB was incubated at 22°C (top) or 37°C (bottom) with different amounts of S1 nuclease for different lengths of time and then analyzed by agarose gel electrophoresis. The sixth and seventh lanes contain undigested supercoiled control and SpeI-digested linear control, respectively. The band that runs slower than the linear band was presumed to correspond to nicked circular plasmid. In the thirteenth lane, after the DNA was digested with 10 units for 24 hours an additional 10 units of S1 nuclease was added and the DNA was incubated for an additional hour. The first and last lanes contain the λDNA/HindIII molecular weight standards. (B) Plasmids with or without the f1 origin (pDIMC8-pfMBP and pRH04-pfMBP, respectively) were incubated with or without S1 nuclease, followed by incubation with or without NcoI, EagI, and SacII and then analyzed by agarose gel electrophoresis. (C) Plasmids with or without the f1 origin (pDIMC8-rbsb and pRH04-rbsb, respectively) were incubated with S1 nuclease or DNaseI, followed by incubation with XmnI and analyzed by agarose gel electrophoresis.

    Techniques Used: Plasmid Preparation, Incubation, Agarose Gel Electrophoresis, Molecular Weight

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    New England Biolabs restriction enzyme digestion
    One percent agarose gel. ( A ) Electrophoresis of the PCR product of the Zika virus recombinant protein E (E-ZIKVre) gene in the commercial vector pUCIDT + E-ZIKVre. Lane 1—Molecular marker 1kb DNA Ladder™ (Life Technologies); line 2–4—PCR product E-ZIKVb; lane 5—negative control. ( B ) Electrophoresis of the enzymatic cleavage digestion products of <t>the</t> <t>pET28a</t> + E-ZIKVre construct with <t>restriction</t> <t>endonucleases</t> <t>NcoI</t> and HindIII. Lane 1—Marker 1kb DNA Ladder™ (Life Technologies); lane 2—digested clone 1; lane 3—undigested clone 1; lane 4—digested clone 2; lane 5—undigested clone 2; lane 6—digested clone 3; lane 7—undigested clone 3; lane 8—digested clone 4; lane 9—undigested clone 4; lane 10—digested clone 5; lane 11—undigested clone 5; lane 12—digested clone 6; and lane 13—undigested clone 6.
    Restriction Enzyme Digestion, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/restriction enzyme digestion/product/New England Biolabs
    Average 96 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    restriction enzyme digestion - by Bioz Stars, 2022-11
    96/100 stars
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    One percent agarose gel. ( A ) Electrophoresis of the PCR product of the Zika virus recombinant protein E (E-ZIKVre) gene in the commercial vector pUCIDT + E-ZIKVre. Lane 1—Molecular marker 1kb DNA Ladder™ (Life Technologies); line 2–4—PCR product E-ZIKVb; lane 5—negative control. ( B ) Electrophoresis of the enzymatic cleavage digestion products of the pET28a + E-ZIKVre construct with restriction endonucleases NcoI and HindIII. Lane 1—Marker 1kb DNA Ladder™ (Life Technologies); lane 2—digested clone 1; lane 3—undigested clone 1; lane 4—digested clone 2; lane 5—undigested clone 2; lane 6—digested clone 3; lane 7—undigested clone 3; lane 8—digested clone 4; lane 9—undigested clone 4; lane 10—digested clone 5; lane 11—undigested clone 5; lane 12—digested clone 6; and lane 13—undigested clone 6.

    Journal: Viruses

    Article Title: Selection and Characterization of Single-Stranded DNA Aptamers of Diagnostic Potential against the Whole Zika Virus

    doi: 10.3390/v14091867

    Figure Lengend Snippet: One percent agarose gel. ( A ) Electrophoresis of the PCR product of the Zika virus recombinant protein E (E-ZIKVre) gene in the commercial vector pUCIDT + E-ZIKVre. Lane 1—Molecular marker 1kb DNA Ladder™ (Life Technologies); line 2–4—PCR product E-ZIKVb; lane 5—negative control. ( B ) Electrophoresis of the enzymatic cleavage digestion products of the pET28a + E-ZIKVre construct with restriction endonucleases NcoI and HindIII. Lane 1—Marker 1kb DNA Ladder™ (Life Technologies); lane 2—digested clone 1; lane 3—undigested clone 1; lane 4—digested clone 2; lane 5—undigested clone 2; lane 6—digested clone 3; lane 7—undigested clone 3; lane 8—digested clone 4; lane 9—undigested clone 4; lane 10—digested clone 5; lane 11—undigested clone 5; lane 12—digested clone 6; and lane 13—undigested clone 6.

    Article Snippet: The E-ZIKVre gene contained in the pUCIDT commercial vector was amplified by PCR with specific oligonucleotides: E-ZIKVre FW (5′-CAT GCC ATG GGC ATT AGG TGC ATA GGC GTT AGC-3′) and E-ZIKVre RV (5′-CCC AAG CTT CTA ATG GTG GTG ATG GTG ATG C-3′) and then cloned into the pET28a expression vector (Novagen, Madison, WI, USA) using restriction endonucleases NcoI (New England Biolabs, Ipswich, MA, USA) and HindIII (New England Biolabs, Ipswich, MA, USA) and the enzyme T4 DNA ligase (Life Technologies, Carlsbad, CA, USA), following the manufacturer’s recommendations.

    Techniques: Agarose Gel Electrophoresis, Electrophoresis, Polymerase Chain Reaction, Recombinant, Plasmid Preparation, Marker, Negative Control, Construct