restriction enzymes bamhi  (New England Biolabs)


Bioz Verified Symbol New England Biolabs is a verified supplier
Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Name:
    BamHI
    Description:
    BamHI 50 000 units
    Catalog Number:
    r0136l
    Price:
    249
    Size:
    50 000 units
    Category:
    Restriction Enzymes
    Buy from Supplier


    Structured Review

    New England Biolabs restriction enzymes bamhi
    BamHI
    BamHI 50 000 units
    https://www.bioz.com/result/restriction enzymes bamhi/product/New England Biolabs
    Average 99 stars, based on 18 article reviews
    Price from $9.99 to $1999.99
    restriction enzymes bamhi - by Bioz Stars, 2020-09
    99/100 stars

    Images

    1) Product Images from "Assembly of evolved ligninolytic genes in Saccharomyces cerevisiae"

    Article Title: Assembly of evolved ligninolytic genes in Saccharomyces cerevisiae

    Journal: Bioengineered

    doi: 10.4161/bioe.29167

    Figure 4. In vivo assembly of synthetic genes by IVOE. Each overlapping region allowed crossover events to occur between fragments giving rise to an autonomously repaired vector containing single and double expression cassettes in the correct orientation. ( A ) and ( B ) Single expression cassettes are constructed in pESC by engineering specific primers containing overhangs to foster in vivo cloning with the linearized plasmid in yeast (pJRoC30 was used as template for Vp or Lac amplifications). ( C ) and ( D ) The pESC constructs obtained in ( A ) and ( B ) were used as scaffolds to assemble Lac and Vp genes under the control of different promoter/terminator pairs. Primers used: (1)-MCS1- Vp / Lac -α-BamHI, (2)-MCS2- Vp -ter-NheI, (3)-MCS2- Lac -ter-NheI, (4)-MCS1- Vp / Lac -α-SpeI, (5)-MCS1- Lac -ter-SacI and (6)-MCS1- Vp -ter-SacI. Black arrows indicate the direction of the transcription process.
    Figure Legend Snippet: Figure 4. In vivo assembly of synthetic genes by IVOE. Each overlapping region allowed crossover events to occur between fragments giving rise to an autonomously repaired vector containing single and double expression cassettes in the correct orientation. ( A ) and ( B ) Single expression cassettes are constructed in pESC by engineering specific primers containing overhangs to foster in vivo cloning with the linearized plasmid in yeast (pJRoC30 was used as template for Vp or Lac amplifications). ( C ) and ( D ) The pESC constructs obtained in ( A ) and ( B ) were used as scaffolds to assemble Lac and Vp genes under the control of different promoter/terminator pairs. Primers used: (1)-MCS1- Vp / Lac -α-BamHI, (2)-MCS2- Vp -ter-NheI, (3)-MCS2- Lac -ter-NheI, (4)-MCS1- Vp / Lac -α-SpeI, (5)-MCS1- Lac -ter-SacI and (6)-MCS1- Vp -ter-SacI. Black arrows indicate the direction of the transcription process.

    Techniques Used: In Vivo, Plasmid Preparation, Expressing, Construct, Clone Assay

    Related Articles

    Polymerase Chain Reaction:

    Article Title: Chlamydomonas reinhardtii hydin is a central pair protein required for flagellar motility
    Article Snippet: .. The PCR product was digested with HindIII and BamHI and ligated into pMAL-cR1 v. 2 digested with the same enzymes (New England Biolabs, Inc.). .. The construct was transformed into E. coli XL1 blue, and, for expression of the maltose-binding∷hydin fusion protein, into BL21.

    Clone Assay:

    Article Title: The development and application of new crystallization method for tobacco mosaic virus coat protein
    Article Snippet: .. Both plasmid PGEX-6P-1 (Novagen) and CP were digested with BamH I (NEB, 10 units/μL)/Xho I (NEB, 10 units/μL) and cloned into the same sites in PGEX-6P-1 (PGEX-6P-1-WT-GST-TMV-CP32 ). ..

    other:

    Article Title: Probing hyper-negatively supercoiled mini-circles with nucleases and DNA binding proteins
    Article Snippet: Materials Escherichia coli topoisomerase I (Ec TopoI), T4 polynucleotide kinase (PNK), calf intestinal phosphatase, T4 DNA ligase, DNAse I, BamHI, BglII and HindIII were from New England Biolabs.

    Article Title: Probing hyper-negatively supercoiled mini-circles with nucleases and DNA binding proteins
    Article Snippet: Escherichia coli topoisomerase I ( Ec TopoI), T4 polynucleotide kinase (PNK), calf intestinal phosphatase, T4 DNA ligase, DNAse I, BamHI, BglII and HindIII were from New England Biolabs.

    Plasmid Preparation:

    Article Title: The development and application of new crystallization method for tobacco mosaic virus coat protein
    Article Snippet: .. Both plasmid PGEX-6P-1 (Novagen) and CP were digested with BamH I (NEB, 10 units/μL)/Xho I (NEB, 10 units/μL) and cloned into the same sites in PGEX-6P-1 (PGEX-6P-1-WT-GST-TMV-CP32 ). ..

    Article Title: Recognition of DNA Termini by the C-Terminal Region of the Ku80 and the DNA-Dependent Protein Kinase Catalytic Subunit
    Article Snippet: .. Kinase assays using plasmid DNA substrates were performed with pcDNA3.1 digested with either XhoI, BamHI, EcoRV, and KpnI or pCAG-GFP digested with XbaI or EcoRI (New England Biolabs). .. The specific sequences recognized by the restriction enzymes and DNA termini generated are shown in .

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    New England Biolabs restriction enzymes bamhi
    Figure 4. In vivo assembly of synthetic genes by IVOE. Each overlapping region allowed crossover events to occur between fragments giving rise to an autonomously repaired vector containing single and double expression cassettes in the correct orientation. ( A ) and ( B ) Single expression cassettes are constructed in pESC by engineering specific primers containing overhangs to foster in vivo cloning with the linearized plasmid in yeast (pJRoC30 was used as template for Vp or Lac amplifications). ( C ) and ( D ) The pESC constructs obtained in ( A ) and ( B ) were used as scaffolds to assemble Lac and Vp genes under the control of different promoter/terminator pairs. Primers used: (1)-MCS1- Vp / Lac <t>-α-BamHI,</t> (2)-MCS2- Vp <t>-ter-NheI,</t> (3)-MCS2- Lac -ter-NheI, (4)-MCS1- Vp / Lac -α-SpeI, (5)-MCS1- Lac -ter-SacI and (6)-MCS1- Vp -ter-SacI. Black arrows indicate the direction of the transcription process.
    Restriction Enzymes Bamhi, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/restriction enzymes bamhi/product/New England Biolabs
    Average 99 stars, based on 18 article reviews
    Price from $9.99 to $1999.99
    restriction enzymes bamhi - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    89
    New England Biolabs bamh1 restriction enzymes
    ]. The cDNA was cloned in PLVX-IRES-ZsGreen-1 vector using flanking primers. Left panel indicates the vector map and right panel represents release of anti-HER2Ab cDNA followed by digestion of PLVX vector with EcoR1 and <t>BamH1</t> restriction endonucleases. The lower panel shows PLVX vector control NSCs and NSCs endoding anti-HER2Ab, sorted based on GFP fluorescence. (B) Temporal secretion of anti-HER2Ab by HER2Ab-NSCs in cell supernatants using ELISA. Note a high production of anti-HER2Ab (~1μg) in 48 hrs. The experiments were repeated three times in triplicates and before in vivo injections of NSCs . (C) Determination of stable assembly of anti-HER2Ab secreted by NSC. First two panel shows SDS-PAGE seperation of trastuzumab (T) and anti-HER2Ab released by NSCs (N) under non-reducing and reducing condition respectively. Next two panels demonstrates western blotting of T and N using anti-Human-HRPO. (D) Quantitative RT-PCR of Nestin, Oct4 and βIII tubulin demonstrating preservation of stemness of HER2Ab-NSCs. The experiments were repeated three times in triplicates. * indicates p
    Bamh1 Restriction Enzymes, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 89/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bamh1 restriction enzymes/product/New England Biolabs
    Average 89 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    bamh1 restriction enzymes - by Bioz Stars, 2020-09
    89/100 stars
      Buy from Supplier

    Image Search Results


    Figure 4. In vivo assembly of synthetic genes by IVOE. Each overlapping region allowed crossover events to occur between fragments giving rise to an autonomously repaired vector containing single and double expression cassettes in the correct orientation. ( A ) and ( B ) Single expression cassettes are constructed in pESC by engineering specific primers containing overhangs to foster in vivo cloning with the linearized plasmid in yeast (pJRoC30 was used as template for Vp or Lac amplifications). ( C ) and ( D ) The pESC constructs obtained in ( A ) and ( B ) were used as scaffolds to assemble Lac and Vp genes under the control of different promoter/terminator pairs. Primers used: (1)-MCS1- Vp / Lac -α-BamHI, (2)-MCS2- Vp -ter-NheI, (3)-MCS2- Lac -ter-NheI, (4)-MCS1- Vp / Lac -α-SpeI, (5)-MCS1- Lac -ter-SacI and (6)-MCS1- Vp -ter-SacI. Black arrows indicate the direction of the transcription process.

    Journal: Bioengineered

    Article Title: Assembly of evolved ligninolytic genes in Saccharomyces cerevisiae

    doi: 10.4161/bioe.29167

    Figure Lengend Snippet: Figure 4. In vivo assembly of synthetic genes by IVOE. Each overlapping region allowed crossover events to occur between fragments giving rise to an autonomously repaired vector containing single and double expression cassettes in the correct orientation. ( A ) and ( B ) Single expression cassettes are constructed in pESC by engineering specific primers containing overhangs to foster in vivo cloning with the linearized plasmid in yeast (pJRoC30 was used as template for Vp or Lac amplifications). ( C ) and ( D ) The pESC constructs obtained in ( A ) and ( B ) were used as scaffolds to assemble Lac and Vp genes under the control of different promoter/terminator pairs. Primers used: (1)-MCS1- Vp / Lac -α-BamHI, (2)-MCS2- Vp -ter-NheI, (3)-MCS2- Lac -ter-NheI, (4)-MCS1- Vp / Lac -α-SpeI, (5)-MCS1- Lac -ter-SacI and (6)-MCS1- Vp -ter-SacI. Black arrows indicate the direction of the transcription process.

    Article Snippet: The ura3 -deficient S. cerevisiae strain BJ5465 ( α ura3–52 trp1 leu2Δ1 his3Δ200 pep4::HIS2 prb1Δ1.6R can1 GAL1 ) was obtained from LGCPromochem, the NucleoSpin Plasmid kit was purchased from Macherey-Nagel, and the restriction enzymes BamHI, NheI, SpeI, SacI, and NotI from New England Biolabs.

    Techniques: In Vivo, Plasmid Preparation, Expressing, Construct, Clone Assay

    ]. The cDNA was cloned in PLVX-IRES-ZsGreen-1 vector using flanking primers. Left panel indicates the vector map and right panel represents release of anti-HER2Ab cDNA followed by digestion of PLVX vector with EcoR1 and BamH1 restriction endonucleases. The lower panel shows PLVX vector control NSCs and NSCs endoding anti-HER2Ab, sorted based on GFP fluorescence. (B) Temporal secretion of anti-HER2Ab by HER2Ab-NSCs in cell supernatants using ELISA. Note a high production of anti-HER2Ab (~1μg) in 48 hrs. The experiments were repeated three times in triplicates and before in vivo injections of NSCs . (C) Determination of stable assembly of anti-HER2Ab secreted by NSC. First two panel shows SDS-PAGE seperation of trastuzumab (T) and anti-HER2Ab released by NSCs (N) under non-reducing and reducing condition respectively. Next two panels demonstrates western blotting of T and N using anti-Human-HRPO. (D) Quantitative RT-PCR of Nestin, Oct4 and βIII tubulin demonstrating preservation of stemness of HER2Ab-NSCs. The experiments were repeated three times in triplicates. * indicates p

    Journal: Stem cells (Dayton, Ohio)

    Article Title: Neural stem cells secreting anti-HER2 antibody improve survival in a preclinical model of HER2 overexpressing breast cancer brain metastases

    doi: 10.1002/stem.2109

    Figure Lengend Snippet: ]. The cDNA was cloned in PLVX-IRES-ZsGreen-1 vector using flanking primers. Left panel indicates the vector map and right panel represents release of anti-HER2Ab cDNA followed by digestion of PLVX vector with EcoR1 and BamH1 restriction endonucleases. The lower panel shows PLVX vector control NSCs and NSCs endoding anti-HER2Ab, sorted based on GFP fluorescence. (B) Temporal secretion of anti-HER2Ab by HER2Ab-NSCs in cell supernatants using ELISA. Note a high production of anti-HER2Ab (~1μg) in 48 hrs. The experiments were repeated three times in triplicates and before in vivo injections of NSCs . (C) Determination of stable assembly of anti-HER2Ab secreted by NSC. First two panel shows SDS-PAGE seperation of trastuzumab (T) and anti-HER2Ab released by NSCs (N) under non-reducing and reducing condition respectively. Next two panels demonstrates western blotting of T and N using anti-Human-HRPO. (D) Quantitative RT-PCR of Nestin, Oct4 and βIII tubulin demonstrating preservation of stemness of HER2Ab-NSCs. The experiments were repeated three times in triplicates. * indicates p

    Article Snippet: Following amplification, the PLVX-IRES-ZsGreen1 plasmid (Clonetech, Mountain View, CA) and the PCR product was digested with EcoR1 and BamH1 restriction enzymes (NEB, Ipswich, MA).

    Techniques: Clone Assay, Plasmid Preparation, Fluorescence, Enzyme-linked Immunosorbent Assay, In Vivo, SDS Page, Western Blot, Quantitative RT-PCR, Preserving